Digital Commons Network^™

Microwave-Assisted Enzymatic Hydrolysis Of Tibetan Sheep Blood Protein And Analysis Of Product Characteristics, Yu Xilu, Gu Xuedong, Chen Yujin, Zhang Hongxin, Ciren Danzhu

Food and Machinery

[Objective] This study aims to utilize fresh Tibetan sheep blood as raw material, investigate the enhancing effect of microwave-assisted technology on enzymatic hydrolysis efficiency, and achieve high-value utilization of Tibetan sheep blood. [Methods] A microwave-assisted trypsin hydrolysis method is employed to enzymatically hydrolyze the proteins. The optimal hydrolysis conditions are established through single-factor experiments and response surface methodology. The resulting hydrolysates are characterized using scanning electron microscopy (SEM) and a fully automated amino acid analyzer. [Results] The optimal hydrolysis conditions are determined as follows: microwave temperature of 40 ℃, microwave time of 50 min, enzymatic hydrolysis time of 240 min, …

Rapid Detection Of Viable Salmonella Based On Specific Phage Infection Combined With Fluorescent Rpa, Liu Daofeng, Zhai Yujie, Tan Yucheng, Shan Shan, Lu Jun, Zhong Ziqing

Food and Machinery

[Objective] To establish a rapid detection method for viable Salmonella based on specific phage infection and recombinase polymerase amplification (RPA) in response to the key challenge that traditional molecular biological detection methods have difficulty in conveniently distinguishing between viable and non-viable foodborne pathogens. [Methods] Taking advantage of the characteristic that O-1 phage only proliferates in viable bacteria, the phage is added to the enriched sample. After further incubation, total DNA is extracted. Fluorescent RPA is used to determine whether the phage has significantly proliferated, so as to judge the presence of viable Salmonella in the original sample. [Results] After optimization, …

Establishment Of An Indirect Competitive Elisa For Sulfadimethoxine, Han Lu, Huang Mingyue, Yu Xiang, Li Sijia, Cao Jinbo, Wang Yao, Kang Huaibin

Food and Machinery

[Objective] To establish an indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) for the detection of sulfadimethoxine (SDM) in animal-derived foods. [Methods] An artificial antigen is synthesized by conjugating SDM with carrier proteins by the diazotization method. A highly sensitive antibody against SDM is prepared by immunizing animals. Based on this, an ic-ELISA is established and the detection system is optimized. Its detection performance is characterized, and spiked recovery tests are carried out. [Results] When the concentration of the coating antigen is 0.500 μg/mL, the dilution ratio of the horseradish peroxidase-labeled goat anti-rabbit secondary antibody is 1:5000, the reaction time of the …

Effect Of Lotus Leaf Powder Addition On Whole Wheat Dough Characteristics And Whole Wheat Bread Quality, Chen Rui, Gong Lingxiao

Food and Machinery

[Objective] To determine the optimum addition of lotus leaf powder in whole wheat flour, thus improving the nutritional value of whole wheat bread while maintaining high quality. [Methods] Rapid viscosity analyzer, rheometer, and texture analyzer are employed to explore the pasting properties of the composite flour, the rheological characteristics of the dough, and the texture properties of the bread. Additionally, the effect of lotus leaf powder on the quality of whole wheat bread is evaluated using a colorimeter, sensory evaluation, and in vitro digestion evaluation. [Results] The analysis reveals that as the lotus leaf powder addition is increased, a significant …

Effects Of Various Extraction Methods On The Performance Of Corn Fiber Gum-Pea Protein Composite Gels, Wu Chenglong, Jiang Zhili, Huang Lilin, Tang Leiming, Sun Xiaohong

Food and Machinery

[Objective] This study aims to investigate the effects of three extraction methods - alkaline extraction, enzymatic extraction, and acid extraction - on the properties of corn fiber gum (CFG)-pea protein composite gels. [Methods] CFG was extracted from corn bran using the three methods. The gel strength, water-holding capacity, rheological properties, and microstructure of the CFG-pea protein composite gels are evaluated. [Results] The CFG extracted by enzymatic extraction increases the gel strength of pea protein gels from (27.20±2.45) g to (65.80±2.67) g, and the water-holding capacity from (40.60±3.40)% to (75.80±5.10)%. Additionally, it forms a more compact and ordered network structure with …

Design And Application Of Low-Temperature Heat Pump Belt Drying Technology In Soy Sauce Residue Drying Process, Liu Shumo, Wang Liangliang, Qi Yan, Luo Zhiqiang, Cao Lei

Food and Machinery

[Objective] To address key issues in conventional soy sauce residue drying processes, including high energy consumption, severe equipment corrosion, and the susceptibility of heat-sensitive nutrients to degradation, a novel integrated drying system was designed and developed to achieve high efficiency, energy savings, and reliable product quality. [Methods] The system integrates key technologies such as spiral air-duct drying and two-stage dust removal. Based on the reverse Carnot cycle principle of a heat pump, a low-temperature drying environment of 40~75 ℃ was established, with the aim of reducing energy consumption while maximizing the retention of the material’s original quality. [Results] The system …

Research Advances In Rice Bran Oil Bioactive Components And Antioxidant Technologies, Tu Yue, Guo Xiongchang, Zhang Wenhan, Cai Lichuang, Liu Jianping

Food and Machinery

Rice bran oil is a unique natural edible oil with healthy benefits and a reasonable fatty acid composition. Containing natural antioxidant components including phytosterols, oryzanol, tocotrienols, and squalene, rice bran oil is characterized by high nutritional value, diverse physiological functions, and excellent frying performance, thus demonstrating broad application potential. This study reviews the research advances in the bioactive components, physiological functions, factors influencing oxidative stability, and antioxidant technologies of rice bran oil, while providing prospects for future development.

Research Progress On Effect Of Salt On Pickling Vegetable Quality, Qi Kai, Li Ziyan, Cheng Wenbo, Fan Mingcong, Zuo Daoxi, Li Yan, Wang Li, Zhang Wenbin

Food and Machinery

Vegetable pickling is a time-honored food preservation and processing method, in which salt (primarily sodium chloride) plays an irreplaceable and core role. This paper systematically reviews the multidimensional effects of curing salt on vegetable quality. It comprehensively covers preservation mechanisms, textural transformations, the formation and conversion of flavor compounds, color stability, and nutritional value changes. Specifically, this article analyzes the decisive role of salt concentration gradients in final product characteristics. Furthermore, it examines the challenges posed by the low salinity, including microbial safety risks, textural deterioration, and flavor imbalances, while proposing corresponding strategies.

Hippocampal “Timing Interval Cells” Exhibit Scalable Dynamics For Explicit Timing, Kori-Anne Citrin

Electronic Theses and Dissertations

The ability to organize experiences in time is fundamental to episodic memory and enables animals to predict and guide future behavior. Hippocampal time cells are thought to provide a neural representation for the temporal component of memory. However, time cells have been observed in working memory tasks where animals were not required to keep track of elapsed time. Therefore, it remains unclear what are the hippocampal patterns of activity during explicit timing. Here, utilizing behavioral perturbations and calcium imaging in dorsal CA1, we identified a previously unknown pyramidal neuron subpopulation that flexibly scales its activity during explicit timing. As head-fixed …

Plant Diversity Modifies Multi-Trophic Interactions In Croplands, Grasslands And Forests, Nian Feng Wan, Muyao Li, Siyuan Shen, Jun Xiang Zhou, Yu Tong Zhu, Ben A. Woodcock, Yue Qing Hu, Shinichi Nakagawa, David Tilman, Andy Hector, Michel Loreau, Peter B. Reich, Matteo Dainese, Forest Isbell, Bernhard Schmid, Kris A.G. Wyckhuys, Geoff M. Gurr, Linsheng Wan, Nico Eisenhauer, Yann Hautier, Anu Eskelinen, Richard D. Bardgett, Paul Kardol, Lauchlan H. Fraser, Qi Su, Shiyun Qiu, Yavanna Aartsma, Ramon Albajes, Matthias Albrecht

All Peer-Reviewed Publications

Multitrophic interactions can strongly influence the structure and functioning of ecosystems, but how plant diversity influences the direction and predictability of multitrophic interactions across agricultural and natural ecosystems remains unclear. Using 149 field studies across five continents, we found that, on average, increasing plant diversity tended to exert differential top-down and bottom-up effects in croplands versus grasslands and forests. Organic and nonorganic croplands exhibited 846 and 148% higher invertebrate natural enemy-to-herbivore abundance ratios under increased plant diversity, consistent with top-down control patterns where predator gains cause herbivore declines, enhancing crop outcomes. In grasslands and forests, increasing plant diversity was associated …

Pharmacological Potential Of Pomelo (Citrus Maxima) Peel: A Bibliometric Review Of Global Research Trends And Future Directions, Indah Zunita Sari, Alma Nur Azizah, Fitriana Nur Hanifah, David Fernando, Agung Endro Nugroho, Abdul Rohman

Journal of Food and Drug Analysis

Pomelo (Citrus maxima) is a widely cultivated tropical fruit with global production reaching approximately 8.55 million tons in 2023. This large-scale production generates a substantial amount of peel waste despite the fact that pomelo peel contains secondary metabolites with promising pharmacological potential. This study aimed to evaluate global research trends related to the pharmacological potential of pomelo peel using a bibliometric and review-based approach, as well as to identify future research directions. Data were collected from the Scopus database and filtered for English-language scientific articles, reviews, and conference proceedings. The analysis was performed using the Biblioshiny and VOSviewer …

Plant By-Products As A Valuble Source Of Natural Antiglycemic Compounds: Economic Aspects And Alternative Raw Material Sources, Tatjana Đorđević, Jelena Vujetić, Isidora Dickov, Diandra Pintać Šarac

Journal of Food and Drug Analysis

Plant by-products, often considered as waste in various agricultural and food processing industries, have emerged as a valuable source of natural antidiabetic or antihyperglycemic compounds. These compounds, derived from plant materials that would otherwise be discarded, have the potential to mitigate the global burden of diabetes and offer sustainable solutions for glycemic control. Prominent groups of natural antidiabetic compounds found in plant by-products are polyphenols, as well as bioactive proteins and peptides. In addition, agro-industrial residues such as wheat bran, oat husks, and fruit peels are excellent sources of carbohydrates that can be incorporated into functional foods to help manage …

Emerging Antifungal Strategies Against Candida Albicans Using Metal–Organic Frameworks And Novel Therapeutics: Opportunities And Challenges, Amanpreet Singh, Sandeep Kumar, Sehrabpreet Singh, Jeewanjot Singh

Journal of Food and Drug Analysis

Candida albicans is an opportunistic fungal pathogen responsible for infections ranging from superficial mucosal disease to life-threatening systemic candidiasis, particularly in immunocompromised hosts. Its pathogenicity is enhanced by morphological plasticity, biofilm formation, and immune evasion, contributing to antifungal resistance. Limitations of existing antifungals have driven interest in emerging therapeutics. This review highlights strategies including metal–organic frameworks (MOFs), host-directed therapies (HDTs), CRISPR-Cas9 gene editing, and phytochemicals. MOFs offer high drug-loading capacity, controlled release, and enhanced penetration into biofilms, showing promise against drug-resistant strains. Selected phytochemicals demonstrate inhibitory effects on virulence traits such as hyphal formation and enzyme secretion. CRISPR-Cas9 …

Systemic Infection Of Beauveria Bassiana In A Stranded Endangered Green Turtle Chelonia Mydas: A Case Report, Ming-An Tsai, I-Chun Chen, Tsung-Hsien Li

The Thai Journal of Veterinary Medicine

The green turtle (Chelonia mydas) is the most frequently encountered marine turtle species in Taiwan. This study describes the postmortem findings of a juvenile green turtle with injuries to the right foreflipper that was rescued off the Taiwan coast and died after 3 months in rehabilitation. Necropsy revealed large, yellow, multifocal caseous lesions involving the right foreflipper, spleen, liver, kidneys, and lungs. Fungal infection of Beauveria bassiana was confirmed by culture, morphological and molecular characterization, phylogenetic analysis, and histopathological examination. This report provides evidence that B. bassiana is associated with a systemic fungal infection which can be fatal …

Cytotoxicity Of Bioactive Peptides From Cowpea (Vigna Unguiculata L. Walp) Against Breast Cancer Cells (Mcf-7), Dian R. Ningsih, Zusfahair Zusfahair, Ely Setiawan, Purwati Purwati, Kasta Gurning, Fitri A. Puspita Sari, Elya Widiawati

Karbala International Journal of Modern Science

Cancer is characterized by dysregulated metabolic signaling pathways, leading to uncontrolled cellular proliferation. Current cancer treatments, which involve surgery, radiotherapy, and chemotherapy, still cause adverse systemic toxicities. Accordingly, one possible effort that can be undertaken is to explore safe anticancer substances from protein sources such as peptides. Bioactive peptides can be isolated by hydrolyzing cowpea (Vigna unguiculata L. Walp) protein with trypsin. The objectives of this study were to isolate and fractionate bioactive peptides from cowpea, to determine the anticancer activity of peptide fractions, to identify active peptide fractions as anticancer agents using Liquid Chromatography High Resolution Mass Spectrometry …

In Vivo Expression Profiling Of Listeria Monocytogenes Internalins (Inlf, Inlp, Inlj) And Their Corresponding Host Receptors (Vim, Afdn, Muc2) Using A Rabbit Ligated Intestinal Loop Model, Nawar Al-Janabi, Nadhim Mushtaq Hashim Al-Bderee, Zahraa M. Madania, Mohanad Jawad Kadhim

Karbala International Journal of Modern Science

Listeria monocytogenes is a Gram-positive pathogen that causes severe infections, particularly in immunocompromised individuals. Internalin genes are important virulence factors that facilitate the bacterium's invasion of host cells. This research aimed to quantify in vivo expression of selected internalin genes (inlF, inlP, and inlJ) and their corresponding host receptors (VIM, AFDN, and MUC2) during L. monocytogenes infection. To achieve this aim, a rabbit ligated intestinal loop model was constructed comprising two intestinal loops. The first loop was injected with 1 mL of L. monocytogenes (1 × 10⁷ CFU/mL), while the second loop was injected with 1 mL of PBS as …

Exogenous Ribosome Incorporation Induces Lineage Transdifferentiation In Intestinal And Liver Cancer Cells, Haoxuan Gu, Nadia Wahyuningsih, Keisuke Yamashita, Shota Inoue, Fuchao Tan, Mani Sharaki, Muhaimin Rifa’I, Kunimasa Ohta

Karbala International Journal of Modern Science

It has previously been shown that exogenous bacterial ribosomes added to somatic cells and various cancer cell lines generate ribosome-induced cell clusters (RICs) capable of transdifferentiating into multiple cellular lineages. However, the scope of ribosome-induced transdifferentiation in cancer cells remains poorly understood. This study aimed to analyze the effects of exogenous ribosome incorporation on human cancer cell lines, specifically the intestinal epithelial cell line Caco-2 and the hepatocellular carcinoma cell line HepG2. Caco-2 and HepG2 cells were cultured with purified bacterial ribosomes in human ES/iPS cell medium after trypsinization, resulting in the formation of ribosome-induced Caco-2 (RICs-Ca) and HepG2 (RICs-He) …

Tapping Into The Ocean’S Hidden Energy: Feasibility Of A 5 Mw Otec Installation In North Bali, Widodo Setiyo Pranowo, Yani Permanawati, Gisela Malya Asoka Anindita, Agung Kurniawan, Albertus Sulaiman, Johar Setiyadi, Safri Burhanuddin, Ivonne Milichristi Radjawane, Hansan Park, Endro Sigit Kurniawan

Karbala International Journal of Modern Science

Indonesia is an archipelagic country surrounded by water and faces energy challenges due to the low use of renewable energy. Among the potential renewable options, marine energy is a particularly suitable resource given the country’s geographical nature. Based on this discussion, seawater temperature can be used as an alternative ocean thermal en-ergy known as ocean thermal energy conversion (OTEC) by using the difference in sea surface and deep-sea water temperatures. Therefore, this study aimed to examine OTEC installations in North Bali waters using closed-cycle OTEC calculations for a 5 MW system. Following this objective, we examined the water conditions by …

Drifting Neurons, Stable Geometry: Population Representations Of Instrumental Behavior In The Dorsomedial Striatum, Arati Sharma

Dartmouth College Ph.D Dissertations

The brain reliably supports learned behaviors over long periods of time despite continual biological changes that alter the activity of individual neurons, and the connections between them. For learned behaviors to persist, the information stored in neural populations must remain accessible even when the activity of individual neurons changes. Understanding how the brain achieves this is a fundamental challenge in neuroscience. The dorsomedial striatum (DMS) is critical for instrumental behavior, encoding information about actions, outcomes, and their relationships. Although extensive work has characterized the role of the DMS during learning and performance, less is known about how task-related information is …

The Effects Of Anti-Gdnf On The Sensory And Autonomic Nervous System In The Heart, Tryphosa Konadu

Honors Theses

Heart disease is the number one leading cause of death; about 1 in every five deaths is due to heart disease. “Heart disease” is a catch-all phrase that encompasses a variety of conditions that affect the heart’s structure and function. Many of these symptoms can be attributed to dysfunction within the parasympathetic, sympathetic, and sensory nervous systems. Neurotrophic factors like GDNF (Glial cell line-derived neurotrophic factor), BDNF (Brain-derived neurotrophic factor), and NGF (Nerve growth factor) promotes neuronal survival, and regeneration of the nerve fibers of the heart, especially sensory and autonomic nerve fibers. Studies have suggested that increased expression of …

Lameness In Dairy Cattle: A Practical Overview, Drew Swartz, Kalen Taylor, Jacob Hadfield, Justin Wyatt Clawson

All Current Publications

This fact sheet provides an overview of lameness in dairy cattle, including what it is, why it matters, and how to recognize and manage it. Information is geared toward producers, consultants, and educators.

Development Of Novel Plasminogen Activators From Fragments Of Streptokinase Enzyme, Vijay G Mr

Theses and Dissertations

Thrombotic disorders such as myocardial infarction, ischemic stroke, pulmonary embolism and deep vein thrombosis remain major causes of mortality and disability worldwide, creating a continuing need for effective, safe and affordable thrombolytic therapy. Streptokinase is one of the most widely used plasminogen activators because of its activity, low cost and broad availability. However, its wider clinical utility is limited by high immunogenicity, allergic reactions, neutralizing antibody formation and poor suitability for repeat administration. This thesis aimed to develop novel streptokinase-derived plasminogen activators by identifying smaller functional fragments that retain thrombolytic activity while potentially reducing immunogenicity.

A literature-guided fragment-based strategy was …

Multi-Sensor Tracking Reveals The Extent Of Nocturnal Migration In Landbirds, Paul Dufour, Raphaël Nussbaumer, Paul Doniol-Valcroze, Yann Rime, Peter Adamík, Susanne Åkesson, Raphaël Arlettaz, Arnaud G. Barras, Nyambayar Batbayar, Andrea Contina

School of Integrative Biological & Chemical Sciences Faculty Publications

Most bird species are diurnal but drastically change their diel cycle of activity to migrate at night. Nocturnal migration has been documented using different methods (e.g., experiments, radar and radio tracking, and acoustic monitoring), but accurately quantifying the proportion of nocturnal versus diurnal flight at the individual and species levels, and understanding how this behavior evolved across the avian tree, has remained methodologically challenging.1,2,3 Such uncertainty is not only of theoretical importance but also limits our ability to mitigate conservation threats, particularly from light pollution and building collisions.4,5,6 With multi-sensor …

Summer Research Internships In Agricultural Biotechnology For Off-Campus Undergraduate Students, Aaron J. Thomas

Funded Research Records

No abstract provided.

Reel Into Fishing!: Providing A Recreational Fishing Education Program For Youth, Amelia Valente, Douglas R. Zemeckis, Lisa Morse, Gene Nigro

Journal of Extension

New Jersey is known as the Garden State, but with over 130 miles of Atlantic coastline, it also has very productive marine ecosystems that offer numerous opportunities for recreational marine fishing. The International Game Fish Association, a nonprofit organization committed to the conservation of game fish and the promotion of responsible, ethical angling practices, collaborated with Rutgers Cooperative Extension to host a youth educational program entitled Reel Into Fishing! The objective is to educate participants through hands-on engagement on ethical fishing practices and marine science to help develop the next generation of responsible anglers.

Three Common Hoof Problems In Dairy Cows: What To Look For And How To Prevent Them, Drew Swartz, Kalen Taylor, Jacob Hadfield, Justin Wyatt Clawson

All Current Publications

This fact sheet covers the three most common hoof lesions in dairy cows and explains why they matter and how to prevent them on your farm.

Digital Dermatitis, Sole Ulcers, And White Line Disease In Dairy Cattle: Pathogens, Diagnosis, And Treatment, Drew Swartz, Kalen Taylor, Jacob Hadfield, Justin Wyatt Clawson

All Current Publications

This fact sheet focuses on three common hoof lesions in dairy cattle: digital dermatitis, sole ulcers, and white line disease, and describes how differences in pathogenesis and risk factors influence diagnosis, prevention, and treatment.

Ai Is Not The Kind Of Thing That Can Ever Be Sentient, Lori Marino

Animal Sentience

Sentience evolved to allow organisms to move through their environment avoiding harms and achieving benefits. Sentience did not evolve from computation. It evolved from valenced feelings. AI (even robots) are not embodied and are not the kind of thing that needs to be sentient. There is no way to get from computations to feelings. They are qualitatively different things. We should avoid being lulled into thinking that an inanimate object designed to engage our propensity for anthropomorphism, like AI, is actually a mind.

Look Out For Our Kind, Christof Koch

Animal Sentience

Strongly supportive overall of Birch’s broad tent Sentience Project, I offer an alternative dyadic, single-class, two-stage process based on estimating the probability for sentience, and the associated confidence, for any individual or taxonomic group. I criticize Birch’s narrow computational framework when addressing the question of algorithmic sentience; there are alternative approaches.

Bull’S-Eye On Borrelia: A Commensal And Consequential Pathogen, Alan L. Gillen

Faculty Publications and Presentations

If Lyme disease causes such urgent and chronic disease issues, where do Borrelia bacteria and ticks fit into a very good creation (Genesis 1)? How did bacteria and ticks change to rely on different hosts and blood for survival? And how are these adaptations informing today’s research in bacteriology, immunology, entomology, and disease ecology? The purposeful placement of Borrelia (Fig. 1) is most likely in the gut of wild mice (Fig. 2), not domesticated mice nor man. The bacteria are communalistic in wild mice, where they may be probiotic for mutual good (Fig. 2)—the Creator made it for good at …