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Population Structure Analysis Of Four Basal Higher-Attines Using Bioinformatic Approaches, Gabriel Mcdanield

Biology Theses

Metapopulations within the family Formicidae are unique among other animals due to the large colonies they build, and the mating strategies required to fertilize a specialized reproductive caste that will often produce young for the life of the colony. One group of ants, the fungus-gardeners (tribe Attini). The population structure of North American non-leafcutting, fungus-gardening ants has been understudied, especially in the southwest of the United States. Additionally, not much is known about their dispersal biology, so the dynamics of dispersal of these species and how they affect population structure is likewise not well known. To shed light on the …

Dog Snapper (Lutjanus Jocu) Neurocranium, Camila Avila Escobar, David W. Kerstetter

All Scans: Kerstetter Fisheries and Avian Ecology 3D Scan Series

No abstract provided.

Effect Of Freezing And Freeze-Thaw Cycles On Equine Serum Insulin Concentration: A Pilot Study, Molly Wallace, Shea Porr, Lydia O'Sullivan

Honors College Theses

Insulin dysregulation is a key component of Equine Metabolic Syndrome (EMS), and is often a predisposing factor to conditions such as laminitis and hepatic lipidosis. Diagnosis of EMS is most often reached through a combination of glucose and insulin measurements in equine serum. Achieving an accurate result of insulin concentration is vital for diagnosis, making proper sample handling essential. The aim of this project was to evaluate the effect of repetitive freeze-thaw cycles on equine serum insulin concentrations. Serum was collected from 10 university-owned horses and split into four treatment groups: Unfrozen, Once Frozen, Twice Frozen, and Thrice Frozen. Unfrozen …

Oligomeric Cystatin C Supports The Immunosuppressive Activity Of Myeloid Cells Through Interaction With Inhibitory Receptors, Chengcheng Zhang, Yubo He, Xiaoye Liu, Jingjing Xie, Meng Fang, Xing Yang, Ryan Huang, Qi Lou, Bufan Li, Ankit Gupta, Cheryl Lewis, Marc I Diamond, Ningyan Zhang, Zhiqiang An, Cheng Cheng Zhang

Faculty, Staff and Student Publications

Amyloid proteins are linked to various diseases; however, their functional roles in immunity and cancer remain unclear. Here, we establish a direct link between oligomeric cystatin C-a cysteine cathepsin inhibitor and a well-characterized amyloidogenic protein-within the tumor microenvironment and the immune inhibitory receptors LILRB2 and LILRB5 on myeloid cells. We demonstrated that human LILRB2 and LILRB5, along with their murine counterpart PIRB, serve as functional receptors for cystatin C oligomers. Engagement of these inhibitory receptors by oligomeric cystatin C enhances the immunosuppressive activity of myeloid cells, leading to T-cell suppression and tumor progression. Deletion of the CST3 gene, which encodes …

Black Grouper (Mycteroperca Bonaci) Neurocranium, Layla Lacelle, David W. Kerstetter

All Scans: Kerstetter Fisheries and Avian Ecology 3D Scan Series

No abstract provided.

Rabies Virus-Vectored Lyme Disease Vaccine Provides Long-Term Protection Against Tick-Transmitted Borrelia Burgdorferi, Shantel Rios, Bibek Bhattachan, Christoph Wirblich, Anisha Chandwani, Kruthi Vavilikolanu, Jacob F. Myers, Chrysoula Kitsou, Utpal Pal, Matthias J. Schnell

Department of Microbiology and Immunology Faculty Papers

Lyme disease (LD) cases have doubled globally. LD is a tick-borne illness caused by the Borrelia burgdorferi sensu lato (Bb). If untreated, Bb can disseminate to distal organs, causing carditis, arthritis, and meningitis. Currently, no FDA-approved human LD vaccine exists on the market. This study used two approaches to incorporate OspA into the rabies virus (RABV) vaccine vector. We used the RABV-glycoprotein tail (RVG tail) and the Hendra virus (HeV) glycoprotein tail (HVG tail) to incorporate OspA, creating BNSP333-OspA-RVG and BNSP333-OspA-HVG, respectively. Both vaccines produced type-1 biased anti-OspA antibodies, but only BNSP333-OspA-HVG induced neutralizing antibodies and protected against Bb infection. …

Developing A General Ai Model For Integrating Diverse Genomic Modalities And Comprehensive Genomic Knowledge, Zhenhao Zhang, Xinyu Bao, Linghua Jiang, Xin Luo, Zheyu Zhang, Jing Yin, Meiqi Zhao, Yichun Wang, Annelise Comai, Joerg Waldhaus, Anders S Hansen, Wenbo Li, Jie Liu

Faculty, Staff and Student Publications

Advances in next-generation sequencing technologies have vastly expanded the availability of diverse genomic, epigenomic, and transcriptomic data, presenting the opportunity to develop a general AI model that integrates comprehensive genomic knowledge into a unified model. Unlike previous predictive models, which are typically specialized to certain tasks, our general AI model unifies a wide range of genomic modalities, such as nascent RNA and ultra-high-resolution chromatin organization, within a multi-task architecture. Using ATAC-seq and DNA sequences as inputs, we incorporated diverse genomic modalities as output, and the model exhibits strong generalizability across different cell types and tissues in all tasks we trained. …

The Unfolded Protein Response-Novel Mechanisms, Challenges, And Key Considerations For Therapeutic Intervention, P M Quan Mai, Tam-Anh Truong, Sai Kumar Samala, Bhoomika Muruvekere Lakshmisha, Prapannajeet Biswal, Khadijeh Koushki, Prudhvi Chand Mallepaddi, Geraldine Vijay, Sunil Krishnan

Faculty, Staff and Student Publications

Background: The unfolded protein response (UPR) is an evolutionarily conserved, synchronized, and orchestrated process triggered by eukaryotic cells in response to endoplasmic reticulum (ER) stress. UPR restores the ER's capacity to handle large protein loads within it, and still fold and process these proteins accurately. Many recent studies have documented the non-canonical roles of the UPR, outside of protein quality control, in the context of lipid metabolism and the immune system in cancer. Cancer cells have been known to hijack the UPR to promote survival and evade immune surveillance. However, the underlying mechanisms remain poorly understood.

Objectives: Here, we critically …

Comparing Machine Learning, Deep Learning, And Reinforcement Learning Performance In Culex Pipiens Predictive Modeling, Wei Yin, Sanad H. Ragab, Michael G. Tyshenko, Teresa Patricia Feria-Arroyo, Tamer Oraby

School of Mathematical & Statistical Sciences Faculty Publications

Several machine learning (ML) and deep learning (DL) methods have been used to predict the presence of species in classification problems. Another set of methods, called reinforcement learning (RL), has been used in training agents to perform various tasks, but not in predicting species distribution. Culex pipiens (Diptera: Culicidae), commonly known as the common house mosquito, is a globally distributed species prevalent in temperate and subtropical regions. They serve as a primary vector for West Nile Virus (WNV), a mosquito-borne pathogen that affects humans and other animals. The study objective is to compare the performance of logistic regression, random forest …

Effect Of Chronic Heat Stress On Duodenal Epithelial Barrier Integrity In Low- And High-Water-Efficient Broiler Chickens, Lulu Liu, Elizabeth S. Greene, Brooklee Roach, Sara Orlowski, Sami Dridi

Poultry Science Faculty Publications and Presentations

Heat stress (HS) has long posed a significant challenge to the poultry industry due to its adverse effects, such as depressed feed intake, decreased growth performance, and increased water consumption. Water efficiency (WE, conversion of water intake into body weight gain), although often neglected, is a key economic and production trait that is significantly affected by HS. Recently, we selected two broiler lines for high WE (HWE) and low WE (LWE) and showed a differential hypothalamic expression of genes involved in water homeostasis regulation. As the gut also plays a significant role in water absorption, the present study aimed to …

Bluefish (Pomatomus Saltatrix) Neurocranium, Courtney R. Schober, Alyssa Olmeda, David W. Kerstetter

All Scans: Kerstetter Fisheries and Avian Ecology 3D Scan Series

No abstract provided.

Morphometric And Meristic Analysis Of Labeo Coubie In Esa-Odo Water Reservoir, Esa-Odo, Osun State Nigeria, Olugbemi V. Ekundare, Funmilayo B. Oyekanmi, Mahmud Sanni, Azeez Aransi, Dorcas O. Owolabi

Tanzania Journal of Science

One of the common species of family Cyprinidae found in Nigeria and West Africa is Labeo coubie. They are of commercial importance in West Africa. This study embarked on the assessment of the length- weight relationship, condition factor, morphometrics and meristic characteristics of L. coubie from Esa-Odo water reservoir, Esa-Odo, Osun State Nigeria. A total of 135 (65 males and 70 females) individual L. coubie collected from fishermen were subjected to morphometric and meristic analysis. Results showed 4.90, 5.46 and 5.24 as the b value for male, female and combined sexes respectively, describing positive allometric growth for all the fish …

Cv: Philip Blankenship (Behavioral Neuroscience), Philip Blankenship

Psychology Department Faculty Curricula Vitae

No abstract provided.

Heritage-Based City Branding Case Study Of Fayoum City, Menna Mounir, Sameir Hammad

Mansoura Engineering Journal

Uniqueness and distinction have become fundamental requirements in promoting and branding cities. Influencing people's perceptions is best achieved by reinforcing the city's identity. This identity is the main component that can transform any ‘space’ into a ‘place’. Promoting a city should extend to highlighting the Heritage, landscape, religious sites, pathways, cuisine, inhabitants, activities, and personal experiences. These elements collectively express the city spirit and enrich visitors’ experience.

This study aims to enhance the image of heritage-based cities by exploring the potential for city branding and leveraging heritage to boost the national economy.

Fayoum was selected as a case study for …

Epigenetic Landscape In Lysosomal Storage Disorders: Mechanisms And Modulation, Andrés Felipe Leal, Harry Pachajoa, Shunji Tomatsu

Department of Pediatrics Faculty Papers

Lysosomal storage disorders (LSDs) are rare substrate-accumulating diseases primarily characterized by mutations in genes encoding proteins involved in lysosomal function, most of which have enzymatic activity. Resulting lysosomal dysfunction leads to the overaccumulation of non- or partially degraded substrates. While it is true that enzyme deficiency is the primary cause of LSDs, the epigenetic alterations in DNA methylation, miRNA expression, and histone modifications appear to be critical mechanisms involved in the pathogenesis of LSDs. As epigenetic marks are, in most cases, reversible, their study becomes vital to developing strategies aimed at reversing epigenome alterations. Although classical therapeutic alternatives aim to …

Reprogramming Tumor Microenvironment Via Systemic Delivery Of Tlr3 Agonist And Manganese Nanoparticle, Young Seok Cho, Xingwu Zhou, Xiaoqi Sun, Ziye Wan, Julia Crowther, Mariko Takahashi, Swetha Kodamasimham, Qi Wu, May Thazin Phoo, Youngseo Na, Kai Han, Zaiye Li, Anna Schwendeman, Steven P Schwendeman, Yu Leo Lei, James J Moon

Faculty, Staff and Student Publications

Toll-like receptor (TLR) agonists, as potent immunostimulatory adjuvants, play a critical role in linking the innate and adaptive immune responses. However, their antitumor effects as cancer immunotherapeutic agents have been limited. Here, we report our finding that manganese ion (Mn2+) potentiates various TLR agonists, leading to robust activation of the TLR pathway and the stimulator of interferon genes (STING) pathway among innate immune cells. In particular, we have observed robust antitumor efficacy after intratumoral administration of a TLR3 agonist and Mn2+. To achieve systemic codelivery of TLR3 agonist and Mn2+, we have developed a low-molecular-weight poly(inosinic:cytidylic acid)-Mn2+ coordination lipid nanoparticle …

Preclinical Efficacy Of Tasquinimod-Based Combinations In Advanced Myeloproliferative Neoplasms In Blastic Phase, Warren Fiskus, Lucia Masarova, Christopher P Mill, Christine E Birdwell, Kaberi Das, Hanxi Hou, John A Davis, Antrix Jain, Anna Malovannaya, Taghi Manshouri, Andrew Dunbar, Surbhi Sharma, Tapan M Kadia, Courtney D Dinardo, Prithviraj Bose, Naveen Pemmaraju, Sanam Loghavi, Xiaoping Su, Raajit K Rampal, Marie Törngren, Kapil N Bhalla

Faculty, Staff and Students Publications

The alarmins, S100A8 (A8) and S100A9 (A9), are low molecular weight proteins belonging to the S100 protein family. A8 and A9 are secreted into the extracellular space and plasma, in which they interact with Toll-like receptor 4, receptor for advanced glycation end products, and CD33. In these studies, we determined the preclinical efficacy of tasquinimod (TQ) against advanced myeloproliferative neoplasm (MPN) cell lines and patient-derived (PD) CD34+ blastic phase (BP; >5% blasts in the peripheral blood) MPN cells. TQ induced loss of viability in cell lines and PD MPN-BP cells, but not in normal CD34+ progenitor cells. In TQ-treated PD …

Bonita - Euthynnus Alletteratus Neurocranium, Daniella M. Acosta Reyes, Matthew Mcmanus, Morgan Michalek, David W. Kerstetter

All Scans: Kerstetter Fisheries and Avian Ecology 3D Scan Series

No abstract provided.

Correcting Class Imbalance Through Synthetic Training Data And 3d Modeling For Carabid Pitfall Trap Sampling, Blair Mirka

Geography ETDs

Crowdsourced biodiversity data provide an accessible foundation for large-scale ecological monitoring, but class imbalance limits automated species identification, particularly for rare taxa. This research explores the use of synthetic training data generated from 3D models of carabid beetle museum specimens to improve detection and classification performance for underrepresented species in crowdsourced datasets. High-resolution 3D models were created to simulate variation in lighting, orientation, and background. These synthetic images were incorporated into convolutional neural network training datasets at varying synthetic-to-real ratios to assess their impact on classification accuracy. Models were evaluated using controlled pitfall-trap imagery to examine the influence of scene …

Nanofiber-Enabled Rapid And Non-Destructive Sensors For Meat Quality And Shelf-Life Monitoring: A Review, Karna Ramachandraiah, Elizabeth M. Martin, Alya Limayem

Food Science Faculty Publications and Presentations

The meat industry faces significant economic losses and environmental impacts due to spoilage and waste, much of which results from inadequate, delayed, or inefficient quality assessment. Traditional methods used for assessing meat quality are often time-consuming, labor-intensive, and lack the ability to provide real-time information, making them insufficient for modern supply chains that demand safety, freshness, and minimal waste. Recent advances in nanotechnology position nanofibers (NFs) as promising materials for addressing these challenges through smart sensing and active packaging. NFs, characterized by their high surface-to-volume ratio, tunable porosity, and small diameter, enable superior encapsulation and immobilization of sensing agents. These …

Genome-Wide Crispr Screens Identify Critical Targets To Enhance Car-Nk Cell Antitumor Potency, Alexander Biederstädt, Rafet Basar, Jeong-Min Park, Nadima Uprety, Rejeena Shrestha, Francia Reyes Silva, Merve Dede, John Watts, Sunil Acharya, Donghai Xiong, Bin Liu, May Daher, Hind Rafei, Pinaki Banerjee, Ping Li, Sanjida Islam, Huihui Fan, Mayra Shanley, Jingling Jin, Bijender Kumar, Vernikka Woods, Paul Lin, Silvia Tiberti, Ana Karen Nunez Cortes, Xin Ru Jiang, Inci Biederstädt, Patrick Zhang, Ye Li, Seema Rawal, Enli Liu, Luis Muniz-Feliciano, Gary M Deyter, Elizabeth J Shpall, Natalie Wall Fowlkes, Ken Chen, Katayoun Rezvani

Faculty, Staff and Student Publications

Adoptive cell therapy using engineered natural killer (NK) cells is a promising approach for cancer treatment, with targeted gene editing offering the potential to further enhance their therapeutic efficacy. However, the spectrum of actionable genetic targets to overcome tumor and microenvironment-mediated immunosuppression remains largely unexplored. We performed multiple genome-wide CRISPR screens in primary human NK cells and identified critical checkpoints regulating resistance to immunosuppressive pressures. Ablation of MED12, ARIH2, and CCNC significantly improved NK cell antitumor activity against multiple treatment-refractory human cancers in vitro and in vivo. CRISPR editing augmented both innate and CAR-mediated NK cell function, associated with enhanced …

Development Of A Targeted Bioprotac Degrader Selective For Misfolded Sod1, Christen G Chisholm, Rachael Bartlett, Mikayla L Brown, Emma-Jayne Proctor, Natalie E Farrawell, Jody Gorman, Fabien Delerue, Lars M Ittner, Kara L Vine-Perrow, Heath Ecroyd, Neil R Cashman, Darren N Saunders, Luke Mcalary, Jeremy S Lum, Justin J Yerbury

Faculty, Staff and Student Publications

The accumulation of misfolded proteins underlies a broad range of neurodegenerative diseases, including amyotrophic lateral sclerosis (ALS). Due to their dynamic nature, these misfolded proteins have proven challenging to target therapeutically. Here, we specifically target misfolded disease variants of the ALS-associated protein superoxide dismutase 1 (SOD1), using a biological proteolysis targeting chimera (BioPROTAC) composed of a SOD1-specific intrabody and an E3 ubiquitin ligase. Screening of intrabodies and E3 ligases for optimal BioPROTAC construction reveals a candidate capable of degrading multiple disease variants of SOD1, preventing their aggregation in cells. Using CRISPR/Cas9 technology to develop a BioPROTAC transgenic mouse line, we …

Tackling The Burden Of Envenomation In Africa: Advances, Challenges, And Strategic Priorities For Enhanced Diagnosis And Treatment, Khawla Ammouch, Nihal Mesmoudi, Noureddine Hammani, Jacob Galan, Abdellah Moustaghfir, Reto Stöcklin, Naoual Oukkache

Human Genetics Publications

Venomous animal envenomation remains a significant but neglected public health problem worldwide, with an estimated 5.4 million cases and up to 138,000 deaths annually. The burden is particularly acute in tropical and subtropical regions, especially sub-Saharan Africa, where rural and vulnerable populations face limited access to effective antivenoms and healthcare. Despite this, comprehensive data on envenomation incidence, clinical management, and outcomes remain scarce, hampering efforts to develop effective prevention and treatment strategies. This review synthesizes current knowledge on venomous animal diversity, epidemiology, and clinical manifestations globally, with a focus on Africa’s unique challenges. We examine critical gaps in antivenom production, …

The Effect Of High-Intensity Interval Training On Visceral Adipose Tissue In Type 2 Diabetes: A Systematic Review, Ivan D. Delgado, Julio C. Delgado, Ryan D. Burns

International Journal of Exercise Science

International Journal of Exercise Science 18(5): 1321-1333, 2025. https://doi.org/10.70252/YEUF2363 Visceral adipose tissue (VAT) is a significant contributor to insulin resistance in type 2 diabetes (T2D). The effectiveness of time-efficient high-intensity interval training (HIIT) in reducing VAT has not been reviewed. A systematic search conducted on PubMed, Embase, and SPORTDiscus up to April 2025 for randomized controlled trials that compared HIIT with non-exercise control and quantified VAT using imaging identified five trials (n = 138) that met inclusion criteria. Risk of bias was assessed using a nine-item modified PEDro scale. Four trials reported significant reductions in VAT after 8-12 weeks of …

Mentoring First-Year Stem Students Through Collaborative Research In The Haynes Scholars Program, Alex Capaldi, Laura Tipton

Annual Symposium on Biomathematics and Ecology Education and Research

No abstract provided.

Representing Ewe-Lamb Contact Dynamics In An Agent-Based Model Of Enzootic Abortion Of Ewes, Kelly R. Buch, Cara J. Sulyok

Annual Symposium on Biomathematics and Ecology Education and Research

No abstract provided.

Commercial Laying Hen Farms' General Characteristics, Environmental Management, And Biosecurity Practices In The Sub-Arid Area Of Algeria, Yacine Kasmi, Nouzha Heleili, Zohra Ahmed Gaid, Brahim Hamad, Leyla Hadef, Manel Merradi

The Thai Journal of Veterinary Medicine

The success of commercial laying hen farms depends on the possibility of managing environmental conditions properly and performing strict biosecurity measures, which would increase productivity, enhance the performance of the flock, and save money, especially in regions characterized by challenging climatic conditions, such as the sub-arid and arid areas. There is, however, very little literature exploring these issues in the Algerian context. The objective of the study was to assess environmental management and biosecurity practices, and the relationship between these and farm size in the Algerian sub-arid area (northeast Algeria). The face-to-face interviews were conducted between January 2024 and April …

Native Bee Assemblages In Prescribed Fire-Managed Prairies: A Case Study From Arkansas, United States, Coleman Z. Little, Neelendra K. Joshi

Entomology and Plant Pathology Faculty Publications and Presentations

Native bee communities in Arkansas remain poorly documented, particularly within fire-managed prairie ecosystems that provide critical habitat for pollinators. This study surveyed bee assemblages at two native prairie remnants in the Arkansas River Valley, one large (Cherokee Prairie Natural Area, CPNA) and one small urban fragment (Jewel Moore Nature Reserve, JMNR), both managed using prescribed fire. Using pan trapping, we recorded 599 individuals representing 96 species across 25 genera, including 49% singletons. Despite differences in size and landscape context, both prairies supported similarly rich bee communities per sample day, with JMNR and CPNA averaging 16.1 and 13.75 species, respectively. However, …

A Compartmental Model For Annual Bat Migration, Elena D. Wheatley, Rhianna Yates, Leah Glasser, Christopher Hay-Jahans

Annual Symposium on Biomathematics and Ecology Education and Research

No abstract provided.

Analyzing Urban Space-Use Of White-Tailed Deer (Odocoileus Virginianus), Szandra A. Péter, Zehidul Hussain, Solena Kronstedt, Amira Roess, Jennifer Mullinax, Travis Gallo, Taylor Anderson

Annual Symposium on Biomathematics and Ecology Education and Research

No abstract provided.