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Influence Of Predation Risk On Elk Feeding Behavior In A Multi-Predator Environment, Rosemarie C. Pugh

All Graduate Theses and Dissertations, Fall 2023 to Present

Sometimes predators don’t just affect prey by killing them, they can also change how prey behave. For example, animals may spend more time watching for danger and less time eating, which could affect their health and survival. In Yellowstone National Park, past research has suggested that elk may reduce feeding because they are trying to avoid predators like wolves and cougars. However, most previous studies have only looked at elk behavior during the daytime and have often looked at responses from single predators rather than multiple.

In this study, I used high-resolution tracking collars equipped with motion sensors to directly …

Effects Of Mineral Supplementation On Ovine Performance, Forage Selectivity, And Rangeland Utilization In Southern Utah, Ryan A. Spurling

All Graduate Theses and Dissertations, Fall 2023 to Present

Sheep producers in the Intermountain West region of the US rely on productive rangeland ecosystems that vary widely in forage composition and nutrient availability. As such, supplementation is often necessary to offset dietary deficiencies. Soils and forages grown on rangelands are known to be highly variable in mineral content. Even so, a third to half of the producers in the Intermountain West choose not to provide a complete mineral supplement. Rangeland environments are often characterized by rugged geographical features that make optimal supplement placement difficult. New advances in technology have allowed us to measure individual animal intake on rangelands via …

Food-Form Delivery Of Spore-Forming Probiotics As An Alternative To Gavage In Animal Models, Aubreyona Faith Migliori Skalka

All Graduate Theses and Dissertations, Fall 2023 to Present

Western dietary patterns are characterized by nutrient-poor, calorie-rich diets and have been linked to poor health outcomes and gut inflammation phenotypes. Probiotics are often taken to support gut health, but many beneficial bacteria do not survive transport through the digestive system to produce any effect. Spore-forming bacteria, such as Bacillus coagulans, are more resilient due to their hardy outer coat which aids in survival through many obstacles encountered in the gastrointestinal tract, including acidic stomach conditions. In research studies, probiotics are usually given to mice through a procedure called oral gavage, where a tube is used to deliver the …

Production Of Hydrolyzed Lactose Syrup From The Acid Whey, Aubrey Jean Arbon

All Graduate Theses and Dissertations, Fall 2023 to Present

Acid whey has gained significant attention within the dairy industry due to its complex matrix and the processing challenges. This by-product contains lactose that can be isolated, hydrolyzed, and further processed to produce hydrolyzed syrup. To optimize lactose utilization, it is essential to fine-tune the kinetics of the β-galactosidase enzymes used for lactose hydrolysis. To analyze sugars in acid whey, and LC-MS-based chromatographic method was developed. Once the method was developed, the kinetics of β-galactosidase were optimized with respect to enzyme concentration, pH, and temperature. The goal was to achieve the highest possible lactose hydrolysis. The hydrolyzed acid whey was …

Mechanisms Of Life History Trade-Offs In The Evolution Of Eusociality, Frances Katherine Hunter

All Graduate Theses and Dissertations, Fall 2023 to Present

When faced with stressors such as pathogen infection, organisms must invest energy in defense or risk death. Because resources are limited, mounting an immune response often comes at the expense of other costly activities such as reproduction. The costs of immunity are shaped by factors including behavior, nutrition, age, and evolutionary history.

Social insects (e.g., ants, bees, and wasps) offer a powerful system for studying these trade-offs because queens in these colonies appear to escape them—living long lives while reproducing prolifically. Bees, which include species that range from those which nest solitarily to those with complex social systems and many …

Effect Of Agroecological Feeding Systems Of Dairy Cattle On The Nutritional Composition Of Milk, Sree Bhavya Immadi

All Graduate Theses and Dissertations, Fall 2023 to Present

How food is produced can influence both its nutritional quality and its impact on the environment. In recent years, regenerative (agroecological) farming practices have gained attention for their potential to improve soil health, biodiversity, and the nutritional value of foods. In dairy production, these practices often involve growing more diverse forage crops, reducing soil disturbance, and relying less on monoculture-grown feed rations. However, there is still limited real-world evidence showing how these practices affect the nutritional composition of milk produced on commercial dairy farms.

This dissertation explores how regenerative feeding and feed production practices influence the nutritional quality of milk, …

Effects Of Serum And Commercial Serum-Free Media On Development Of In Vitro Produced (Ivp) Embryos In Cattle, Kyla Miller

All Graduate Theses and Dissertations, Fall 2023 to Present

Serum-containing media has been shown to increase incidence of large offspring syndrome and to alter gene expression, protein synthesis, and cell signaling. These factors together can result in increased pregnancy loss when combined with assisted reproduction technologies. However, there are very few direct comparisons of commercial serum-free media (IVF Bioscience™) and serum-containing media on both IVF and cloned embryo development in cattle. The aim of this study was to determine the role of serum in oocyte maturation and subsequent embryo development. Three culture systems, including in vitro maturation (IVM) and in vitro culture (IVC), were used in this study. Both …

Exploring The Correlation Between Malnutrition And Food And Nutrition Insecurity In Inflammatory Bowel Disease, Heather Burr

All Graduate Theses and Dissertations, Fall 2023 to Present

Inflammatory Bowel Disease (IBD) may increase the risk of malnutrition because of nausea, vomiting, diarrhea and restrictive eating. In addition, patients with IBD may experience food and nutrition insecurity. Therefore, we conducted an online survey to assess risk of malnutrition and food and nutrition insecurity among adult patients with IBD in outpatient and community settings. Patients were recruited between July and December 2025, aiming to reach adults in the Rocky Mountain Region diagnosed with IBD. Data collected included demographics, health-related factors, risk of nutrition insecurity, risk of food insecurity, and barriers and facilitators to dietary management of IBD.

Fifty-seven patients …

Mitogenomic And Physiological Comparison Between Wild And Domesticated Congeners, Nicholas Enriquez

Open Access Theses & Dissertations

Domestication is a process in which wild populations are morphologically and/or behaviorally manipulated through selective breeding to become optimized for living in captivity. Consequently, many traits become quickly and significantly diverged from their ancestral, wild lineages, a process known as part of domestication syndrome. Captive breeding of mallards (Anas platyrhynchos) was one of the last major successful domestication events resulting in dozens of recognized breeds today. Many breeds were optimized for either ornamental or agricultural purposes (i.e., meat and/or egg production), but the game-farm mallard was specifically bred for sport hunting. In North America, the use of game-farm mallards to …

Development Of A High-Throughput Framework For Studying Choice-Movement Dynamics In Freely Moving Rats Across Motivational And Pharmacological States, Atanu Giri

Open Access Theses & Dissertations

This thesis develops and applies high-throughput behavioral assays and analysis pipelines to study how external contingencies and internal state shape rodent decision-making. First, it introduces RECORD (Reward-Cost in Rodent Decision-making), a modular platform that combines 3D-printed arenas, microcontroller-based control, and closed-loop trial structure to deliver graded rewards and costs in a foraging-like environment. RECORD supports scalable data collection across multiple arenas and a software stack for parsing, databasing, and extracting spatiotemporal behavioral features and psychometric choice functions. Using this framework, the thesis quantifies how animals integrate sucrose reward magnitude with aversive light cost, revealing structured individual differences and sex-dependent movement …

Metabolomics In The Dryland Critical Zone: The Role Of Soil Metabolites In Phosphorus Acquisition, Kalpana Kukreja

Open Access Theses & Dissertations

Earth's Critical Zone (CZ) plays a key role in sustaining life through carbon, water, and nutrient cycling from the top of the canopy to the groundwater. A key feature of critical zone research is the integration of multiple interdisciplinary techniques. An emerging set of techniques that can add to this approach is the field of metabolomics, the quantification and study of comprehensive metabolite profiles. In this dissertation, I apply metabolomics to address knowledge gaps in dryland critical zone ecosystems, specifically how soil metabolite dynamics influence biogeochemical processes, and with a particular emphasis on phosphorus (P) cycling in the Chihuahuan Desert. …

Mapping The Lateral Hypothalamic Area: Chemoarchitecture, Connectivity, And Activation In Motivated Learning And Reward, Vanessa Inez Navarro

Open Access Theses & Dissertations

The lateral hypothalamic area (LHA) is a large and complex hypothalamic region with diverse functions across four main rostrocaudal divisions. Despite its size and diversity, disagreements over how to define LHA subdivisions (e.g., Swanson atlas lists 17 subdivisions) make it challenging to fully characterize its structure and function. Across these subregions, neuronal connectivity varies, indicating they serve different behavioral functions. Consistent with this, particular LHA neuronal pathways have been linked to reward-related responses, emphasizing the region's roles in motivation, feeding, and cognition. Current approaches to classifying the LHA have primarily relied on neurochemical markers or gene-directed manipulations, often without integrating …

Salience Network Circuit Dynamics In Distress Tolerance And Drug-Seeking Vulnerability, Marina Alicia Smoak

Open Access Theses & Dissertations

The psychological distress experienced during drug withdrawal can lead individuals with substance use disorder to abandon their goal of abstinence. Numerous human studies demonstrate that an inability to persist in challenging, goal-directed behavior while experiencing psychological distress is associated with multiple stages of substance use, including relapse. This behavioral construct is referred to as low distress tolerance (DT). Rodent models of DT similarly show that low DT predicts elevated cocaine relapse, particularly when DT is assessed following extended abstinence. This abstinence period corresponds with the incubation of craving, a phenomenon characterized by progressively increased cocaine-seeking over time. Importantly, relapse vulnerability …

Fluoxetine Exposure During Adolescence Alters Prefrontal Cortex Signaling And Nociceptive-Related Behavior In Adulthood, Anapaula Themann

Open Access Theses & Dissertations

The long-term neurobiological consequences of adolescent antidepressant exposure remain poorly understood, despite the substantial increase in selective serotonin reuptake inhibitor (SSRI) prescription rates in youth. Therefore, the goal of this dissertation was to determine whether adolescent exposure to the SSRI fluoxetine (FLX) produces lasting molecular and behavioral adaptations, using rodents as a model system. To do this, in Chapter 2, I assessed whether adolescent FLX exposure (postnatal day [PD] 35-49) alters signaling molecules associated with survival and synaptic plasticity (extracellular signa- regulated kinase [ERK]) within the adult prefrontal cortex (PFC) - a brain region that plays a central role in …

High-Resolution Mapping Of Brainstem Feeding Circuits: A Framework For Targeted Neuroscience Tools, Geronimo Tapia

Open Access Theses & Dissertations

The neural control of energy balance is mediated in part by distributed brainstem circuits that integrate metabolic, visceral, and hormonal signals to coordinate appropriate motivated behaviors and autonomic responses. Despite extensive study, the precise neuroanatomical architecture regulating metabolism remains incompletely defined within standardized atlas frameworks. This work addresses that gap through complementary studies examining an acute glycemic challenge methodology known to stimulate feeding, the acute delivery of an anorexigenic-associated neurohormone, and the chemoarchitecture of a major feeding-related region, the parabrachial nucleus. First, utilizing 2-deoxy-D-glucose (2-DG) induced glucoprivation, we map the rapid response of dorsal medullary neuronal populations with high spatial …

Soil Microbial Responses To Artifical Light At Night, Randall L. Walker

Open Access Theses & Dissertations

Global change, driven by both natural processes and human activities, has significantly transformed ecosystems. Urbanization and artificial light at night (ALAN) are key contributors, disrupting natural light cycles and influencing biological rhythms, biodiversity, and ecosystem functions. Thus far, there remains a gap in knowledge of how soil ecosystems are impacted by ALAN. This study uses a laboratory incubation to investigate the effects of ALAN on soil microbial communities and functions across urban and natural ecosystems. Briefly, soil was collected from eight sites around El Paso, Texas and neighboring New Mexico representing urban and rural light exposure. Soil was then subjected …

Characterization Of The Antiviral Activity Of Mouse Schlafen Proteins, Wendy Paola Alvarez Leal

Open Access Theses & Dissertations

The goal of this work was to characterize the anti-viral role of mouse Schlafen8 and Schlafen9. Expression of these proteins is induced by type I interferon in response to viral infections. Importantly, their human functional orthologs exhibit anti-viral functions, and the structural element required for these activities are conserved in Schlafen8 and Schlafen9. Therefore, it is expected that these proteins will also impair viral replication. An important limitation in this field is that the anti-viral function of human SLFNs has been evidenced only in in vitro cellular models. No informative mutations have been described in human populations so far. Thus, …

Exploring A 'Fishy' Solution: Uncovering Human Norovirus Using A Zebrafish Model, Sahaana Chandran

Graduate Theses and Dissertations

Human norovirus (HuNoV) is the leading cause of acute gastroenteritis worldwide; however, the lack of robust laboratory culture systems and reliable animal models has limited progress in understanding viral replication and developing effective intervention strategies. While alternative models such as the zebrafish system have been explored, gaps remain in evaluating their ability to support replication across diverse HuNoV strains and in identifying factors that influence infectivity. This dissertation provides a comprehensive investigation into the use of the zebrafish model for studying viral replication, host interactions, and potential inhibition strategies. Initial studies focused on optimizing viral delivery methods using Tulane virus …

Bats And Bacteria: Infection Dynamics At The Human-Wildlife Interface, Isabella Katherine Deanglis

Graduate Theses and Dissertations

As the second most diverse mammalian order, bats host an array of pathogens, some of which are known to infect humans. Synanthropic bats share spaces with humans, resulting in bat-borne pathogen exposure. While most bat-borne disease research has focused on viral pathogens, bats host numerous bacterial pathogens with zoonotic potential. By integrating a global meta-analysis with field studies in Sub-Saharan Africa, I characterize the structural attributes that facilitate shared interfaces, the bacterial pathogens present in synanthropic bats of rural Kenya, and the physiological and environmental dynamics influencing odds of infection in these synanthropic bats. Finally, I evaluate the clinical relevance …

Beyond The Brain: Environmental Forces Driving Α-Synuclein Misfolding And Toxic Aggregation In Parkinson's Disease, Emily Mccarthy

Celebrating Scholarship and Creativity Day (2018-)

Parkinson's disease (PD) is an advanced neurodegenerative disorder characterized by the degeneration of dopaminergic neurons and the proliferation of protein aggregates within the brain. This capstone thesis investigates the unique biochemical environment of the gastrointestinal tract and whether it contributes to the formation of distinctive α-Synuclein (αSyn) aggregates affecting PD. Key promoters of αSyn aggregation include genetic mutations, oxidative stress, post-translational modifications, interactions with metal ions, and dopamine (DA) oxidation. These factors result in diverse structures such as oligomers, protofibrils, and fibrils, which result in unique functional outcomes, with oligomeric conformations leading to the highest level of neuronal toxicity. Evidence …

The Effect Of Liquid Foliar Fertilizer And Pruning Combinations On The Growth Of Productive Phase Arabica Coffee Plants (Coffea Arabica L.), Syifa Djuita Putri, Alvito Krishna Balapradhana, Mira Ariyanti, Intan Ratna Dewi Anjarsari, Yudithia Maxiselly

Jurnal Kultivasi

In recent years, productivity decline in arabica coffee has become a real issue in Indonesia. This decay has been brought about by various external and internal factors that are beyond human control. Various factors, including suboptimal cultivation practices, take part in this process. The objective of the present study was to evaluate the effect of pruning combined with foliar fertilizer application (coconut water and sugarcane molasses) and 6-benzylaminopurine (BAP) on the vegetative growth of arabica coffee plants during the production phase. The research was conducted at Ciparanje Experimental Field, Universitas Padjadjaran, using a randomized complete block design with seven treatments …

Creating A Unique Hemoglobin-Based Oxygen Carrier, Clayton Fuller

Celebrating Scholarship and Creativity Day (2018-)

The purpose of this thesis is to explain the current state of alternative oxygen carriers and propose a new hemoglobin-based oxygen carrier (HBOC) as a blood substitute/ blood therapeutic. Blood donation has drawbacks: risk of spread of infection, short storage time, and blood typing, and the current shortage. There are several possible modifications for hemoglobin to create a carrier (HBOC) which address specific issues related to allowing free hemoglobin to exist extra cellularly. One issue to address is vasoconstriction caused by nitric oxide scavenging, which has been solved by increasing the molecular weight of the HBOC via (co)polymerization, or by …

Sex Or Survival: Does Wounding Elicit A Somatic Reproductive Tradeoff In Narnia Femorata Males?, Lillian E. Burbulis

Departmental Honors & Graduate Capstone Projects

Animals must partition resources into different life history traits resulting in tradeoffs because resources are finite. A tradeoff is the allocation of resources to one trait over another. Theory states that energy is allocated in a hierarchical fashion. For example, somatic maintenance should be a higher priority than reproduction, resulting in more resources being allocated to somatic maintenance compared to reproductive traits. The metabolic investment hypothesis states that energy must follow a hierarchical order prioritizing somatic maintenance over reproduction. Another hypothesis for energy allocation is the terminal investment hypothesis. This hypothesis states that energy will be allocated to reproduction over …

Ai-Driven Multispectral Drone Monitoring For Afforestation In Arid Environments, Hesham Morgan, Ali Elgendy, Brandon Tran, Tamer Ismail, Mohamed M. Moursy, Yehya Kh. Shehadeh, Ahmed Elgharib, Ahmed Abdullah Al-Dughairi, Ali El Mubarak, Khaled Allam Harhash, Hesham El-Askary

Mathematics, Physics, and Computer Science Faculty Articles and Research

Monitoring large-scale afforestation projects in arid and semi-arid environments requires accurate, high-resolution, and repeatable methods to assess tree survival and growth. In this study, we integrated unmanned aerial vehicle (UAV) multispectral imaging with an advanced object detection framework to evaluate vegetation establishment in the Shuayb Al-Budai afforestation site, part of the Imam Turki bin Abdullah Royal Natural Reserve, Kingdom of Saudi Arabia (KSA). Multispectral datasets were acquired using a MicaSense Altum-PT sensor and processed through a masked Region-based Convolutional Neural Network (RCNN) with two backbone architectures: ResNet-101 and VGG19-BN. The Mask R-CNN–ResNet-101 model achieved superior performance, with an overall accuracy …

A Two-Phase Hippocampal Code Linking Time And Distance Estimation To Action, Raphael Heldman

Electronic Theses and Dissertations 2020 - Present

A critical component of spatial navigation is the ability to estimate elapsed time or distance traveled. How the brain generates such estimates and links them to behavior remains unclear. In this thesis, we identify a previously unrecognized population coding strategy for time or distance in hippocampal CA1, a region critical for navigation and memory. Using novel behavioral paradigms in head-fixed mice combined with electrophysiological recordings, we show that this code is carried by two functionally distinct subpopulations of pyramidal neurons, termed PyrUp and PyrDown, whose firing rates evolve in opposite directions during navigation. Both populations exhibit a common two-phase dynamic: …

Assessment Of Morphological Differences And Genetic Variation In Head Shape Of Florida Pompano Trachinotus Carolinus, Neriah Nicholas

Electronic Theses and Dissertations 2020 - Present

Genomics research can inform selective breeding programs to improve broodstock and seedstock quality for the U.S. warmwater marine finfish aquaculture industry. Florida pompano Trachinotus carolinus serves as a model species for the industry due to its commercial value and ability to spawn in captivity. This study examined a growth abnormality observed in commercially produced juveniles in partnership with Aquaco Farms LLC. Twenty fish were collected, culled, and euthanized by Aquaco staff, then classified into two head types: “normal” and “concaved”. Eleven morphological measurements were recorded before and after freezing to assess head variation and storage effects. DNA from pectoral fin …

Elucidating The Role Of The Gastrointestinal Microbiome And Diet In Dopamine Release In Relation To Attention-Deficit/Hyperactivity Disorder., Meaghan Mcdonald

Celebrating Scholarship and Creativity Day (2018-)

Currently affecting 340-580 million people worldwide, ADHD is a neurodevelopmental disorder that affects how the brain controls attention, behaviour and impulses often beginning in childhood and continuing into adulthood. ADHD is known to be associated with monoamine neurotransmitters including dopamine. This work aims to uncover the ways in which metabolism of monoamine precursors in the gastrointestinal microbiome influences dopamine release in the brain. Previous studies have indicated that children with ADHD display distinct gut bacteria profiles with unique microbiota species that result in the disruption of monoamine precursor pathways of phenylalanine and tyrosine metabolism. Emphasis on our western diet and …

Time And Dose Dependent Effects Of Adipose-Derived Stromal Vascular Fraction Exosomes On Human Dental Pulp Stem Cell Proliferation, Sylva Dinie Alinda, Anggraini Margono, Indah Julianto, Dini Asrianti Bagio

Journal of Dentistry Indonesia

Background: Cell proliferation is essential for tissue repair and immunomodulation in regenerative dentistry. Adipose-derived stromal vascular fraction (AD-SVF) is a promising autologous source for tissue engineering. Its exosomes (AD-SVF Exo), as cell-free products stable under ischemic conditions, represent an ethical and attractive modality for dental tissue regeneration and broader regenerative applications. Prior study indicates that AD-SVF Exo can enhance human dental pulp stem cell (hDPSC) migration and proliferation, particularly at low concentrations, but their detailed time- and dose-dependent proliferative effects remain unclear. This study aimed to investigate the time- and dose-dependent effects of low-concentration AD-SVF Exo on hDPSC proliferation …

Comparative Analysis Of The Quality Of Life Of Schizophrenic Patients On Antipsychotic Treatment Between Aripiprazole Monotherapy And Combination Of Risperidone-Clozapine At Mental Hospital In West Java, Taufiqurahman Taufiqurahman, Sani Asmi Ramdani Lestari, Ardilla Kemala Dewi

Pharmacology and Clinical Pharmacy Research

Quality of life (QoL) is a critical outcome measure in schizophrenia management, yet antipsychotic side effects may negatively impact patients' well-being. Objective: This study aimed to compare the QoL of schizophrenia patients receiving aripiprazole monotherapy versus a risperidone-clozapine combination at West Java Mental Hospital. An analytical observational study with a cross-sectional design was conducted at the outpatient clinic of West Java Mental Hospital from March to May 2025. The Indonesian version of the WHOQOL-BREF questionnaire was used to assess QoL. A total of 58 outpatients with schizophrenia who met the inclusion criteria were enrolled, comprising 28 patients in the aripiprazole …

Comparison Of Chop And R-Chop For Treatment Non-Hodgkin Lymphoma, Yogyakarta, Indonesia: A Cost-Effectiveness Analysis, Woro Supadmi, Rizky Gustinanda, Lalu M. Irham, Riat El Khair

Pharmacology and Clinical Pharmacy Research

CHOP and R-CHOP are commonly used chemotherapy regimens for Non-Hodgkin Lymphoma (NHL). While R-CHOP has demonstrated clinical benefits, its higher cost raises concerns regarding economic efficiency, particularly in middle-income countries such as Indonesia. This study aimed to compare direct medical costs and clinical outcomes of CHOP and R-CHOP regimens among NHL patients treated at a national referral hospital in yogykarta. A retrospective cohort study was conducted using medical record data of NHL patients who received CHOP or R-CHOP chemotherapy at Dr. Sardjito General Hospital Yogyakarta, between 2020 and 2023, with follow-up extended until April 30, 2025. Clinical outcomes included overall …