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Ultrastructure Of Sarcocystis Sporocysts From Passerine Birds And Opossums: Comments On Classification Of The Genus Isospora, Edith D. Box, Alan A. Marchiondo, Donald W. Duszynski, C. Patrick Davis

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Sporocysts were obtained from the feces of opossums (Didelphis virginiana) which had been fed muscles of passerine birds (Molothrus ater and Cassidix mexicanus) infected with Sarcocystis. Sporocysts were examined by phase microscopy and scanning and transmission electron microscopy. Ridges on the surface of the sporocysts outlined four plates. Thin sections of the sporocyst wall showed thickened regions and gaps interpreted as cross sections of the ridges. The sporocyst wall has four major layers with a thick, granular inner layer which resembles the inner layer of sporocysts of related species. Excystation structures are discussed as a …

Parasite Population Regulation: Lethal And Sublethal Effects Of Leptorhynchoides Thecatus (Acanthocephala: Rhadinorhynchidae) On Hyalella Azteca (Amphipoda), Richard L. Uznanski, Brent B. Nickol

Harold W. Manter Laboratory of Parasitology: Faculty Publications

The hypothesis that Leptorhynchoides thecatus populations are regulated by mortality of heavily infected intermediate hosts, Hyalella azteca, was examined experimentally. Mortality related to the infection process could not be demonstrated because no survivorship difference occurred between amphipod groups exposed to L. thecatus and unexposed control groups after 24 hours Likewise, amphipod mortality could not be associated with infection intensity during this period. Amphipod mortality, growth, and infection intensity were monitored for 25 days after a 24-hour exposure period. Cystacanths were infective to fish after 25 days. Survivorship of exposed amphipods was at least as great as that of unexposed …

Isospora Californica (Protozoa: Eimeriidae) In Peromyscus Maniculatus (Cricetidae) From White Sands National Monument, New Mexico, Lillian F. Mayberry, John R. Bristol, Donald W. Duszynski

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Isospora californica (Protozoa: Eimeriidae) in Peromyscus maniculatus (Cricetidae) from White Sands National Monument, New Mexico

This report constitutes a new geographic record for Isospora californica and adds a micrograph of the coccidium, which the original description did not include. The latter is important because to correctly identify coccidia, a photograph as well as a drawing is at times necessary.

An Examination Of The Red Blood Cell Alloantigentic System In The Chinese Hamster, Kathryn Elliget Blashfield

Masters Theses

This research was done to determine whether any ABH-like blood group system was present in Chinese hamsters (Cricetulus griseus). The population of animals included 35 hamsters, 20 "normal" or outbred (10 males; 10 females) and 15 diabetic from The Upjohn Company's colony of inbred diabetic animals (8 males; 7 females; BE and BA lines). Animal sera and erythrocytes were compared by: hyperimmunization of rabbits (10 New Zealand White) with hamster erythrocytes; screening hamster erythrocytes and plasmas with human blood diagnostic typing antisera (Ortho Diagnostics), anti-H lectin, anti-A₁ lectin and human red cells of various types; absorption and elution of …

Immune Responses Of Rats To Antigens Of Moloney Leukemia Virus, Frances B. Soderberg, Susan G. Tai, Joe M. Jones

Journal of the Arkansas Academy of Science

No abstract provided.

Hemogregarines In The Red-Eared Slider, Chrysemys Scripta Elegans (Wied) From Arkansas, Chris T. Mcallister, Anthony W. King

Journal of the Arkansas Academy of Science

No abstract provided.

Arapaho Prairie, Arthur County, Nebraska: Approximate Ap Grid For Gis, Kathleen H. Keeler, A. T. Harrison, L. S. Vescio

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Grid map of field sites at Arapaho Prairie in Arthur County, Nebraska. Scale 1 5/16" = 1/4 mile. Shows permanently marked vegetation quadrats, blowouts and ravine washouts, roads, and 100' contour intervals. Part of the map was destroyed by mice. What remains of the map as of 2013 is shown.

Three Proteocephalid Cestodes From Colombian Siluriform Fishes, Including Nomimoscolex Alovarius Sp.N. (Monticelliidae: Z Ygobothriinae), Daniel R. Brooks, Thomas L. Deardorff

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Three species of proteocephalid cestodes were collected from Colombian siluriform fishes. Goezeella siluri Fuhrmann, 1916 is reported from Ageneiosus caucanus for the first time, and Colombia is a new locality. Goezeella siluri has a metascolex, biloculate suckers, and cortical gonads. Proteocephalideans possessing uniloculate suckers and cortical gonads represent two groupings. Those species possessing metascolices represent the genus Spatulifer Woodland, 1934, containing the species surubim, piramutab, piracatinga , rugosa , and rugata. Those lacking metascolices belong in Monticellia LaRue, 1911, comprising coryphicephala, lenha, megacephala, and spinulifera. New combinations include Spatulifer piramutab for Goezeella piramutab, S. piracatinga for Monticellia piracatinga, …

Cestodes In Four Species Of Euryhaline Stingrays From Colombia, Daniel R. Brooks, Monte A. Mayers

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Seven species of cestodes are reported from coastal stingrays in the vicinity of Cartagena, Colombia, Acanthobothrium colombianum sp,n. from Aetobatis narinari resembles A . paulum and A. mathiasi but differs by having relatively shorter, more squared proglottids at the end of the strobila, a pre-equatorial genital pore, relatively less elongate ovarian lobes in terminal proglottids, and less prominent apical suckers and pads. Acanthobothrium urotrygoni sp.n. from Urotrygon venezuelae most closely resembles A . olseni in bothridial hook length but differs in cirrus sac size, resembles A. southwelli in cirrus sac size but differs in bothridial hook length, and differs from …

Occurrence Of Quadrigyrus Torquatus Van Cleave, 1920 (Acanthocephala) In North-Central Colombia, South America, Richard L. Buckner, Daniel R. Brooks

Harold W. Manter Laboratory of Parasitology: Faculty Publications

During June and July of 1975 and 1976, fish of the Magdalena River basin in Colombia, South America, were examined for acanthocephalans, A total of 16 species of fish were collected from the departments of Caldas and Bolivar. Worms were fixed in AFA and stained in Mayer's carmalum,

New Host And Locality For Kathlania Leptura (Rudolphi) (Nematoda: Oxyurata: Kathlanidae), Daniel R. Brooks, Jack Frazier

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Marine testudines are known to host two species of kathlanid nematodes, Kathlania leptura (Rudolphi, 1819) Travassos, 1918 and Tonaudia tonaudia (Lane, 1914) Travassos, 1918, Each species has been reported infrequently but exhibits a wide geographic distribution. Both K. leptura and T. tonaudia have been reported from Ceylon and from the Mediterranean Sea near Egypt, and K. leptura has been reported also from the coast of Brazil (Skrjabin, Shikhobalova, and Lagodovskaya, 1964, in Skrjabin (ed,) Essentials of Nematodology, vol. 13, part 3, Academy of Sciences of the USSR, English translation, 1976, TT75-50011, U.S. Department of Commerce, NTIS, Springfield, Virginia 22151), This …

Taxonomy And Biology Of North American Species Of Goezia (Nematoda: Anisakidae) From Fishes, Including Three New Species, Thomas L. Deardorff, Robin M. Overstreet

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Three new species of Goezia from fishes in North America are described and supplemental data for G. minuta and several unidentified adults and larvae are presented. Males, especially their caudal papillae, are necessary to identify most species. For the new species, G. pelagia sp. n. from Rachycentron canadum and Chaetodipterus faber in the northern Gulf of Mexico possesses 12- 19 preanal, two para-anal, and four postanal pairs of papillae; G. kliksi sp. n. from Pogonias cromis in Lake Borgne, Louisiana, has 10-16 preanal, two para-anal, and five postanal pairs of papillae, and G. sinamora sp. n. from Tilapia aurea, …

Redescription Of Diandrya Composita Darrah, 1930 (Cestoda: Anoplocephalidae) From Nearctic Marmots (Rodentia: Sciuridae) And The Relationships Of The Genus Diandrya Emend., Robert L. Rausch

Harold W. Manter Laboratory of Parasitology: Faculty Publications

According to the original description of Diandrya composita Darrah, 1930 (the type and only species of the Nearctic genus Diandrya Darrah, 1930) , this cestode would possess a combination of organs, the "interproglottidal glands" and "pedunculated prostate glands," otherwise unknown in any member of the Anoplocephalidae. Neither of these organs is present in D. composita . This species is redescribed and the generic diagnosis is emended accordingly. Diandrya composita has its closest affinities with cestodes of the genus Andrya Railliet, 1893, from which it differs significantly only in reduplication of the reproductive organs.

Two New Species Of Cestode (Trypanorhyncha, Eutetrarhynchidae) From The Yellow-Spotted Stingray, Urolophus Jamaicensis, Kinga J. Kovacs, Gerald D. Schmidt

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Eutetrarhynchus thalassius sp.n. and Eutetrarhynchus caribbensis sp.n. were collected from the spiral valves of three yellow-spotted stingrays, Urolophus jamaicensis, in Discovery Bay, Jamaica. Both species most closely resemble Eutetrarhynchus geraschmidti Dollfus, 1974. Eutetrarhynchus thalassius differs from E. geraschmidti in the arrangement, sizes, and shapes of tentacular armature, Eutetrarhynchus caribbensis differs from E. geraschmidti and E. thalassius on the basis of metabasal armature. The tentacular armature of E. geraschmidti is redescribed,

Cestode Parasites Of Some Venezuelan Stingrays, Monte A. Mayes, Daniel R. Brooks

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Forty-seven stingrays representing 8 species collected in Venezuelan coastal waters were examined for cestode parasites. Specimens of 18 tetraphyllidean, lecanicephalidean, and trypanorhynchan cestode species were collected, 3 described as new. Rhinebothrium margaritense sp. n. from Dasyatis guttata and D. americana most closely resembles R. spinicephalum and R. tetralobatum but differs by having 3-6 rather than only 2 testes per proglottid and a different number of bothridial loculi. Rhodobothrium paucitesticulare sp. n. from Rhinoptera bonasus possesses fewer than half as many testes per proglottid as any other known member of the genus. Dioecotaenia campbelli sp. n. differs from D. cancellata by …

Epicharinema Keralense N. Gen, N. Sp., And Comments On Atylenchinae And Ecphyadophorinae (Nemata : Tylenchidae), Dewey J. Raski, Armand R. Maggenti, Puthenkandathil K. Koshy, Varghese K. Sosamma

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Epicharinema keralense n. gen., n. sp. is described from nematodes collected from soil about the roots of coconut palm in Kerala, India. This genus is related to Ecphyadophora and Ecphyadophoroides but differs from both by virtue of its greater length; larger stylet, spicules and gubernaculum; and its well-developed metacorpus with a prominent valve. E. keralense n. gen., n. sp. also has an elongate, sinuous cleft connected to the amphid which, with the valve in its metacorpus, indicates these belong to the family Tylenchidae. Relationship with Atylenchus and Eutylenchus is also considered and comments on the subfamilies Atylenchinae and Ecphyadophorinae are …

Diatoms In The Gills Of The Commercial White Shrimp, Robin M. Overstreet, Susan Safford

Harold W. Manter Laboratory of Parasitology: Faculty Publications

A white shrimp from Galveston, Texas, is the first reported case of a crustacean internally infected by a diatom. Even though more than one species occurred in debris on and between gill filaments, only individuals of Amphora sp. occurred within gills. To determine if a related diatom would easily reproduce within the shrimp and cause. a host response similar to that observed, we injected cultured specimens of A. coffaeformis into white shrimp. Under the experimental conditions, individuals of that species did not divide, but they elicited an extensive melanistic host-response.

Cephalic Papillae Of Giant Kidney Nematode Dioctophyma Renale (Goeze, 1782) And Comparison With Eustrongylides Spp., J. R. Lichtenfels, P. A. Madden

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Cephalic papillae of third- and fifth-stage Dioctophyma renale and fourth- and fifth-stage Eustrongylides spp. were found to be of three kinds in addition to the amphids. In all the stages of both genera studied, six papillae were in an internal circle, six in an external circle, and eight to 10 in two lateral fields of four or five each between the internal and external circles. Amphids were closely associated with the externolateral papillae. Another porelike papilla was found between the ventroventral papillae in all but fifth-stage D. renale. In third-stage D. renale, lateral rows of somatic papillae were …

Epicharinema Keralense N. Gen, N. Sp., And Comments On Atylenchinae And Ecphyadophorinae (Nemata: Tylenchidae), Dewey J. Raski, Armand R. Maggenti, P. K. Koshy, V. K. Sosamma

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Epicharinema keralense n. gen., n. sp. is described from nematodes collected from soil about the roots of coconut palm in Kerala, India. This genus is related to Ecphyadophora and Ecphyadophoroides but differs from both by virtue of its greater length ; larger stylet, spicules and gubernaculum; and its well-developed metacorpus with a prominent valve. E. keralense n. gen., n. sp. also has an elongate, sinuous cleft connected to the amphid which, with the valve in its metacorpus, indicates these belong to the family Tylenchidae. Relationship with Atylenchus and Eutylenchus is also considered and comments on the subfamilies Atylenchinae and Ecphyadophorinae …

Dirofilaria Immitis In The Wild Canids Of Illinois, Thomas J. Kick

Masters Theses

The heartworm, Dirofilaria immitis, is widespread in dogs in North America and has been reported in wild canids in many states. This is a report of this parasite in hearts removed from coyote, red fox and gray fox carcasses collected in Illinois. A total of 415 coyotes had a significantly higher infection rate (23.6%) than 225 red foxes (3.6%) and 99 gray foxes (3.0%). Infection rates in red foxes and coyotes were significantly higher in or near the Wabash Border Natural Division along the eastern side of the state, suggesting a relationship between infection rates and this region. The …

A New And Unusual Eimerian (Protozoa: Eimeriidae) From The Liver Of The Gulf Killifish, Fundulus Grandis, Donald W. Duszynski, Mobashir A. Solangi, Robin M. Overstreet

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Oocysts and sporocysts of Eimeria funduli sp. n. are described from the Gulf killifish, Fundulus grandis, on the basis of light microscopy, transmission and scanning electron microscopy, and location in the liver of infected hosts. The spherical sporulated oocysts of E. funduli isolated from liver tissue measure 20-31(25) μm across with ovoid sporocysts 9-11 × 5-7 (10 × 6) μm. A micropyle, polar granule, and oocyst residuum are absent, but sporocysts have Stieda and substieda bodies, a few residual granules, and 10-25 (15) unique projecting structures with expanded distal portions that we term “sporopodia.” Sporopodia 1-3 (2) μm high …

Agar Plate Culture Of Selected Trypanosomatidae, Amy Doran Keppel

School of Biological Sciences: Dissertations, Theses, and Student Research

Summary

The study of trypanosomatid biology would benefit greatly from the acquisition of a tool for culturing such as those used in microbiology. Agar plate culture has been described several times, but until the present its potential for use in this area has been underestimated. The previous work has demonstrated that this culture method may be used to culture a wide variety of species, that these species give rise to identifiable colony phenotypes, and that these are stable regardless of the life cycle stage used to initiate the culture. It was also shown that colonies could be initiated from the …

Detection Of Unique Tumor-Associated Proteins In The Urine Of Patients With Transitional Cell Carcinoma By High Resolution Two-Dimensional Polyacrylamide Gel Electrophoresis, Robert David Lehman

Biological Sciences Theses & Dissertations

A two-dimensional polyacrylamide gel electrophoretic technique was developed for the detection of unique tumor-associated proteins in urine of patients with transitional cell carcinoma (TCC) of the urinary bladder. This technique yielded high resolution of polypeptides in the urine of cancer patients. Urine was chosen as a source of tumor-associated components because the location of the tum or should release already solublized tum or substances in to the urine.

Urinary proteins were concentrated and then analyzed by the two - dimensional electrophoretic technique using isoelectric focusing in the first dimension and sodium dodecylsulfate - polyacrylamide gel electrophoresis in the second dimension. …

Concomitant Infections With The Parasites Trypanosoma Musculi Kendall, 1906 And Trichinella Spiralis (Owen, 1835) Raillet, 1896 In The Laboratory Mouse, Musmusculus, Joan Chew Liang Tan

Loma Linda University Electronic Theses, Dissertations & Projects

Four groups of female Swiss Webster mice were injected intraperitoneally with similar volumes of normal saline, sonicated Trypanosoma musculi and saline, sonicated trypanosomes and Freund's Complete Adjuvant (FCA) and FCA. When the mice became immune and thus responded negatively to a T. musculi challenge, sera were collected and administered to four other groups of mice to establish passive immunity in these animals.

An established dose of innoculum of Trichinella spiralis larvae (8.1 larvae/gram of body weight) was given to all the mice by gastric gavage. These mice were killed 26 days later and the musculature digested with artificial gastric juice …

The Effects Of Temperature And Ph On The Differentiation Of Trypanosoma Lewisi (Kent, 1880) Laveran & Mesnil, 1901, In A Defined Medium, Carolann R. Rosario

Loma Linda University Electronic Theses, Dissertations & Projects

Trypanosoma lewisi is an arthropod-transmitted protozoan parasite found in the bloodstream of rats. In its life cycle it undergoes cellular differentiation. It is primarily found as the trypomastigote form in the rat and as the epimastigote form in the arthropod, the rat flea. Various studies have been done on hemoflagellates to determine the factors responsible for stimulating the differentiation from one form to the other. Included in these factors are pH, temperature, and protein fractions from fetal calf serum. Recent efforts have also been aimed at developing simple or defined media for physiological or biochemical studies on hemoflagellates. The purpose …

Alveolar Hydatid Disease In Minnesota: First Human Case Acquired In The Contiguous United States, William G. Gamble, Martin Segal, Peter M. Schantz, Robert L. Rausch

Harold W. Manter Laboratory of Parasitology: Faculty Publications

A 56-year-old woman from southwestern Minnesota underwent an extended left hepatic lobectomy to remove a large multinodular mass with a necrotic central cavity. The clinical, serological, and pathological findings led to the diagnosis of alveolar hydatid disease, and specific identification of Echinococcus multilocularis was achieved by growing mature larvas in voles inoculated intraperitoneally with tissue from the hepatic lesions. The patient probably acquired her infection some years previously from pet cats or dogs that had become infected by ingesting infected rodents. In North America E. multilocularis is enzootic in the northern tundra zone of Alaska and Canada. Since 1964 the …

Nueva Especie Del Género Acanthobothrium Van Benedem 1849; Cestode: Tetraphyllidea De Myliobatis Peruvianus Garman 1913, Ruperto Severino L., Luz Sarmiento B.

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Se describe Acanthobothrium gonzalesmugaburoi n.sp. (Cestoda: Tetraphyllidea: Onchobotriidae) hallado en la válvula espiral de 10 ejemplares de "raya aguila" Myliobatis peruvianus Garman, 1913, procedentes del litoral peruano. Difiere de las demás especies del género, por el número y distribución de los testículos y por la longitud total del gancho. Huésped y localidad se señalan por primera vez.

Ryegrass Toxicity Organism Found On Other Grasses, D L. Chatel, J. L. Wise, A. G. Marfleet

Journal of the Department of Agriculture, Western Australia, Series 4

Yellow slime disease caused by Corynebacterium sp. has recently been found in three grass species.

The diseased grasses were found in the field growing with each other and with affected toxic annual ryegrass.

Notes On Seasonal Variation And Infectivity Of Haemogamasus Reidi, A Vector Of Hepatozoon Griseisciuri, On Eastern Gray Squirrels, Sciurus Carolinensis, Mohammed Bashiru

Masters Theses

Five hundred and twenty-two squirrels were trapped and retrapped from a population in Montgomery County, Maryland during 1974-1975. One hundred and forty-one squirrels were found to harbor an average of 10.6 mites (Range 175-1). While male squirrels outnumbered females (79 vs 62) females harbored more mites than males (829 vs 666). During the spring the average number of mites per captured squirrel increased to an average of 18.1 per squirrel. The average declined to 6.8, 4.8 and 3.3 per squirrel in summer, fall and winter respectively. Throughout the collection period adult female mites were by far more numerous on hosts …

Flystrike In Sheep : Secondary And Tertiary Flies Striking Sheep In Western Australia, N Monzu

Journal of the Department of Agriculture, Western Australia, Series 4

Unlike primary species of blowflies, the secondary and tertiary species of flies cannot initiate a strike, but their activity in the presence of the primary blowflies becomes significant in the death of struck sheep. This is particularily so when the hairy maggot blowfly is involved. Future management of sheep flocks to minimise deaths may require recognition of these secondary and tirtiary flies.

This article presents some basic facts about these flies.