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Review Of Phylogeny, Ecology, And Behavior By Daniel R. Brooks And Deborah A. Mclennan, University Of Chicago Press, 1991, John J. Janovy Jr. Apr 1992

Review Of Phylogeny, Ecology, And Behavior By Daniel R. Brooks And Deborah A. Mclennan, University Of Chicago Press, 1991, John J. Janovy Jr.

John J. Janovy, Jr. Publications

Review of Phylogeny, Ecology, and Behavior by Daniel R. Brooks and Deborah A. McLennan, University of Chicago Press, 1991.


Parasitology As An Interpretive Tool In Archaeology, Karl J. Reinhard Jan 1992

Parasitology As An Interpretive Tool In Archaeology, Karl J. Reinhard

Karl Reinhard Publications

Parasitological studies of archaeological sites can be used to interpret past behavior and living conditions. During the 1980s problem-oriented research into prehistoric- and historical-period parasitism developed and resulted in thefield of archaeoparasitology. Archaeoparasitology attempts to integrate parasite data into archaeological theory and interpretation. Within the last decade, four major archaeoparasitological laboratories emerged. They developed interpretive frameworks that apply parasitological data to a remarkable variety of prehistoric behaviors. Parasite remains can be used to reconstruct aspects of diet, health, and other behaviors such as transhumance and trade. Finally. analysis of the distribution of parasite remains can be used to interpret aspects …


Understanding The Importance Of Eperythrozoon Ovis Infection In Sheep, Marc Kabay Jan 1992

Understanding The Importance Of Eperythrozoon Ovis Infection In Sheep, Marc Kabay

Journal of the Department of Agriculture, Western Australia, Series 4

Assigning priorities for research on sheep diseases relies in part on understanding the prevalence and economic impact of each disease.

Eperythrozoon ovis infection in sheep, for example is a common, but not costly, disease.


The Sheep Lice Detection Test, Peter Morcombe Jan 1992

The Sheep Lice Detection Test, Peter Morcombe

Journal of the Department of Agriculture, Western Australia, Series 4

Sheep farmers would agree it is difficult to find sheep lice when carrying out an inspection.

The lice detection test makes this task easier and helps farmers decide how to control lice. The test also helps community liaison groups with eradication of lice infestations in specific areas, and provides data for epidemiologists to monitor changes in prevalence of lice and efficacy of treatments. Tests are much cheaper than flock inspection on the farm.

The present lice detection test was developed by the Australian Wool Testing Authority (A WTA) and introduced as an integral part of the Western Australian Sheep Lice …


On The Trail Of The Native Budworm, Kevin Walden Jan 1992

On The Trail Of The Native Budworm, Kevin Walden

Journal of the Department of Agriculture, Western Australia, Series 4

Native budworm, the worst pest of lupins, is a bit of a uiill-o-the-unsp. Some years the native budworm moths go virtually unnoticed. In others, they appear in plague proportions without warning - in these years, the cost to growers in lost yield runs into millions of dollars.

If an early warning system can be developed to predict the extent of moth flights into lupin crops, and this seems possible, major crop losses could be averted.

The Department of Agriculture has started research to develop a system of predicting the size of spring flights of native budworm moth. We need to …


Morphology Of The Synlophe And Genital Cone Of Parostertagia Heterospiculum (Trichostrongylidae) With Comments On The Subfamilial Placement Of The Genus, Eric P. Hoberg, J. Ralph Lichtenfels Jan 1992

Morphology Of The Synlophe And Genital Cone Of Parostertagia Heterospiculum (Trichostrongylidae) With Comments On The Subfamilial Placement Of The Genus, Eric P. Hoberg, J. Ralph Lichtenfels

Harold W. Manter Laboratory of Parasitology: Faculty Publications

The genus Parostertagia is referred to the Cooperiinae with reference to a suite of shared characters of the synlophe and genital cone postulated as derived homologies for the subfamily. The genus has traditionally been relegated to the Graphidiinae or Ostertagiinae, but unique structural attributes of the synlophe and genital cone indicate close affinities with the Cooperiinae. The synlophe of Parostertagia heterospiculum is characterized by a relatively low number of ridges in the cervical zone (16-20 at the excretory pore), a frontal axis of orientation, sequential increase in ridges posteriorly, lateral addition of ridges, minuscule lateral-most ridges and hypertrophy of specific …


Historical Ecology As A Research Program, Daniel R. Brooks, Deborah A. Mclennan Jan 1992

Historical Ecology As A Research Program, Daniel R. Brooks, Deborah A. Mclennan

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Modern evolutionary biology is the descendant of two theories proposed by Darwin. First, all organisms are connected by common genealogy, and second, the form and function of organisms is closely tied to the environments in which they live. Of these two theories, the role of the first (phylogeny) in evolutionary explanations has been diminishing in some fields, most notably in ecology and ethology. However, the last ten years have witnessed the beginning of a reversal in this trend. With increasing frequency, ecologists (Wanntorp et aI., 1990; Maurer and Brooks, submitted), ethologists (Dobson, 1985; Huey and Bennett, 1987; Mclennan et aI., …


Coprolite Analysis: A Biological Perspective On Archaeology, Karl J. Reinhard, Baughn M. Bryant Jr Jan 1992

Coprolite Analysis: A Biological Perspective On Archaeology, Karl J. Reinhard, Baughn M. Bryant Jr

Karl Reinhard Publications

The most remarkable dietary remains recoverable from archaeological contexts are coprolites. Coprolites are desiccated or mineralized feces that are preserved in sheltered and open sites in arid regions, primarily in the New World. These dietary remains are remarkable from several perspectives. They typically contain a variety of macroscopic and microscopic remains that form interrelated data sets for the reconstruction of diets. Because contexts containing coprolites are typified by excellent preservation, the remains coprolites contain tend to be in better states of preservation than dietary remains recovered from nonfecal deposits. Coprolites also contain the well-preserved remains of intestinal parasites and pathogens …


Vertebral Pathology In Prehistoric And Historic Skeletons From Northeastern Nebraska, Karin L. Sandness, Karl J. Reinhard Jan 1992

Vertebral Pathology In Prehistoric And Historic Skeletons From Northeastern Nebraska, Karin L. Sandness, Karl J. Reinhard

Karl Reinhard Publications

Vertebral pathology has long been a useful criterion for anthropologists in the assessment of activity patterns, stress, and general health of extinct peoples. This method of analysis, however, has never been applied to the peoples of the Nebraska Great Plains. This study is the first to concentrate on the indigenous Native Americans of this region, examining the spinal pathology present in the prehistoric and historic skeletal remains. Pathology present in the form of spondylolysis, Schmorl's nodes, osteophytosis (degenerative disc disease), and osteoarthritis (degenerative joint disease), provides evidence to suggest differing activity patterns and levels of stress in Plains groups before …


Dan Canyon Burial: A Piii Burial In Glen Canyon National Recreation Area, Steve Dominguez, Karl Reinhard, Kari L. Sandness, Cherie A. Edwards, Dennis Danielson, F. A. Calabrese, Chris Kincaid Jan 1992

Dan Canyon Burial: A Piii Burial In Glen Canyon National Recreation Area, Steve Dominguez, Karl Reinhard, Kari L. Sandness, Cherie A. Edwards, Dennis Danielson, F. A. Calabrese, Chris Kincaid

Karl Reinhard Publications

The Dan Canyon burial was discovered at a time when the philosophy, ethics, and legislation concerning the study of human remains are in a state of flux. A number of important sensitive issues germane to managers, archeologists, and American Indians are discussed in the introduction. The subsequent analysis provides a detailed scientific account of these remains and a glimpse of a segment of a people's past lifeway while remaining sensitive to the wishes of the American Indians.

The burial and associated grave goods of site 42SA21339 were exposed by wave action in a location frequented by boaters at the Glen …


Report Of Round Table On Genetics And Systematics Of Trichinella (Icopa Vii, Paris), Robert L. Rausch, J. Ralph Lichtenfels, K. D. Murrell Jan 1992

Report Of Round Table On Genetics And Systematics Of Trichinella (Icopa Vii, Paris), Robert L. Rausch, J. Ralph Lichtenfels, K. D. Murrell

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Report of the Round Table on Genetics and Systematics of Trichinella from the International Congress of Parasitology (ICOPA) VII, 1990, Paris.


New Species Of Rhabdochona Railliet, 1916 (Nemata: Rhabdochonidae) From Rainbow Trout In California Streams, Armand R. Maggenti, Fawzia Abdel-Rahman, I. Cid Del Prado Vera Jan 1992

New Species Of Rhabdochona Railliet, 1916 (Nemata: Rhabdochonidae) From Rainbow Trout In California Streams, Armand R. Maggenti, Fawzia Abdel-Rahman, I. Cid Del Prado Vera

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Three new species of Rhabdochona Railliet, 1916 are described and illustrated from Salmo gairdneri Richardson (rainbow trout) in freshwater streams in California: Rhabdochona californiensis n. sp., R. paxmani n. sp., and R. satmonis n. sp. Rhabdochona californiensis n. sp. is characterized by 14 anteriorly directed teeth in the prostome, egg devoid of filaments or floats, male and female tail terminus with a single mucro, left (long) spicule slender with a moderate distended podoid terminal end, spicular ratio 1:3.8. Rhabdochona paxmani n. sp. is characterized by 10 teeth in the prostome, eggs with polar floats, left (long) spicule slender with podoid …


Microphallus Fonti Sp. N. (Digenea: Microphallidae) From The Red Swamp Crawfish In Southern United States, Robin M. Overstreet, Richard W. Heard, Jeffrey M. Lotz Jan 1992

Microphallus Fonti Sp. N. (Digenea: Microphallidae) From The Red Swamp Crawfish In Southern United States, Robin M. Overstreet, Richard W. Heard, Jeffrey M. Lotz

Harold W. Manter Laboratory of Parasitology: Faculty Publications

A new species of digenean, Microphallus fonti, is described from the red swamp crawfish in Louisiana, USA. It has a small pharynx and a rudimentary gut like M. opacus and possibly related species from crayfishes, but it differs from them by its relatively large male copulatory papilla and a conspicuous metraterm.


Phylogeny, Historical Biogeography, And Ecology Of Anophryocephalus Spp. (Eucestoda: Tetrabothriidae) Among Pinnipeds Of The Holarctic During The Late Tertiary And Pleistocene, Eric P. Hoberg, Ann M. Adams Jan 1992

Phylogeny, Historical Biogeography, And Ecology Of Anophryocephalus Spp. (Eucestoda: Tetrabothriidae) Among Pinnipeds Of The Holarctic During The Late Tertiary And Pleistocene, Eric P. Hoberg, Ann M. Adams

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Phylogenetic systematic analyses of Anophryocephalus spp. resulted in a single most-parsimonious cladogram (consistency index: 80%). Cladograms for pinniped hosts (phocids and otariids) and Anophryocephalus spp. were highly incongruent, corroborating a hypothesis for colonization as a dominant determinant of parasite diversification. Phoca (Pusa) spp. in the Atlantic basin are postulated as the initial hosts; range expansion for hosts and parasites into the Pacific basin through the Arctic (ca. 3.0-2.5 million years ago) was followed by radiation of Anophryocephalus spp. among Phoca spp. and subsequent colonization of otariids (Eumetopias jubatus as typical hosts; ca. 2.0 million years ago). Host …


Miscellaneous Publication 39/91 - How To Eradicate Virulent Footrot From Your Property. A Farmer's Guide., Dianne Hopkins Dec 1991

Miscellaneous Publication 39/91 - How To Eradicate Virulent Footrot From Your Property. A Farmer's Guide., Dianne Hopkins

Animal production and livestock published reports

The first thing to understand about virulent footrot is that it can occur on any farm in the medium to high rainfall zone.

People often associate footrot with properties managed by inefficient farmers. This is a belief based on fallacy. Many good farmers have found the disease in their sheep, including high profile stud producers.

Footrot can strike anywhere and without warning.

The important thing to remember is, that once it is found, getting to know the disease and understanding it will help you beat it. This manual, the video and your stock inspector will help you eradicate footrot from …


Phylogenetic Analysis Of Alloglossidium Simer, 1929 (Digenea: Plagiorchiiformes: Macroderoididae) With Discussion Of The Origin Of Truncated Life Cycle Patterns In The Genus, Joseph P. Carney, Daniel R. Brooks Dec 1991

Phylogenetic Analysis Of Alloglossidium Simer, 1929 (Digenea: Plagiorchiiformes: Macroderoididae) With Discussion Of The Origin Of Truncated Life Cycle Patterns In The Genus, Joseph P. Carney, Daniel R. Brooks

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Alloglossidium comprises 9 species of North American plagiorchiiform digeneans using ictalurid catfish, freshwater crustacea, and hirudinid leeches as definitive hosts. Two hypotheses about the evolution of this array of definitive hosts were examined using phylogenetic systematic analysis. Two most parsimonious trees, based on 15 homologous series derived from morphological data, each indicated the two species utilizing ictalurid catfish definitive hosts are basal members of the group, whereas the two species using freshwater crayfish definitive hosts and the five utilizing leech definitive hosts each comprise relatively derived monophyletic sister groups. The results suggest that species using crustaceans as definitive hosts are …


Introduction Of Marilyn E. Scott As The Henry Baldwin Ward Medalist For 1991, Daniel R. Brooks Nov 1991

Introduction Of Marilyn E. Scott As The Henry Baldwin Ward Medalist For 1991, Daniel R. Brooks

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Transcript of the introduction of Marilyn E. Scott as the American Society of Parasitologists Henry Baldwin Ward Medalist for 1991, by Daniel R. Brooks.


The Niche Of Salsuginus Thalkeni, A Gill Parasite Of Fundulus Zebrinus, John J. Janovy Jr., Mary Ann Mcdowell, Michael T. Ferdig Oct 1991

The Niche Of Salsuginus Thalkeni, A Gill Parasite Of Fundulus Zebrinus, John J. Janovy Jr., Mary Ann Mcdowell, Michael T. Ferdig

John J. Janovy, Jr. Publications

Distribution of a monogenean parasitic helminth Salsuginus thalkeni on the gills of the fish Fundulus zebrinus is described by calculation of mean positions and niche breadths on the linear spatial resource gradients gill filament length, gill arch length and arch number. All distributions are given for parasites in the presence and absence of various combinations of potential competitors, namely the 6 other parasite species that occupy the same host species. Filament niche breadth was narrowest in the absence of potential competitors; breadth on arch was widest in the presence of potential competitors. Breadth on both resources was correlated positively with …


Parasites And Sexual Selection: A Macroevolutionary Perspective, Deborah A. Mclennan, Daniel R. Brooks Sep 1991

Parasites And Sexual Selection: A Macroevolutionary Perspective, Deborah A. Mclennan, Daniel R. Brooks

Harold W. Manter Laboratory of Parasitology: Faculty Publications

The Hamilton-Zuk hypothesis postulates a causal link between parasitism and the evolution of epigamic traits by intersexual selection. Oversimplified assumptions about basic parasite biology, ambiguous formulation of the hypothesis, and poor communication between ethologists and parasitologists have hampered its testing. The hypothesis is supported at the microevolutionary level if females show significant preference for lightly or uninfected males, if intensity of infection reflects host resistance to parasites that depress host fitness by causing disease, and if intensity of infectionis related to the degree of epigamic development. It must be shown that particular parasites cause disease, that the host population is …


Syndisyrinx Evelinae (Marcus, 1968) N. Comb., From The Rock-Boring Urchin, Echinometra Lucunter, From St. Barthélemy, Lynn Ann Hertel, Donald W. Duszynski Aug 1991

Syndisyrinx Evelinae (Marcus, 1968) N. Comb., From The Rock-Boring Urchin, Echinometra Lucunter, From St. Barthélemy, Lynn Ann Hertel, Donald W. Duszynski

Harold W. Manter Laboratory of Parasitology: Faculty Publications

The umagillid turbellarian Syndesmis evelinae, originally described from unnamed Caribbean sea urchins, is redescribed and placed in the genus Syndisyrinx. Syndisyrinx evelinae n. comb. was found in 8 of 32 (25%) Echinometra lucunter from the Bay of St. Jean, St. Barthélemy; 6 of the 8 (75%) urchins with S. evelinae also had Syndisyrinx collongistyla. The major difference between the original description and the worms described here is the structure of the bursal valve. The valve was originally described as having a thickness between the seminal receptacle and the seminal bursa; in fact, the bursal valve has 2 …


Purification And Characterization Of The Prostate Cancer-Associated Antigen: 7e11-C5, Qi Feng Jul 1991

Purification And Characterization Of The Prostate Cancer-Associated Antigen: 7e11-C5, Qi Feng

Theses and Dissertations in Biomedical Sciences

A murine monoclonal antibody (MAb), 7E11-C5, recognizes a prostate organ-specific antigen. Under reducing conditions, the 7E11-C5 antigen exhibited major 100-Kd and minor 70-Kd bands, suggesting that the antigen comprises two peptide chains. The antigen was detected in pooled normal human seminal plasma, xenograft tissue extract of a prostate adenocarcinoma cell line, LNCaP, and tissue extracts of benign prostatic hyperplasia (BPH) and prostate carcinomas, but was not identified in any of the non-prostate tissue extracts tested. Antibodies of the three major prostate-associated antigens (prostatic acid phosphatase (PAP), prostate specific antigen (PSA) and prostate secretory protein (PSP)) were unable to block the …


Use Of Pollen Concentration In Paleopharmacology: Coprolite Evidence Of Medicinal Plants, Karl Reinhard, Donny L. Hamilton, Richard H. Hevly Jul 1991

Use Of Pollen Concentration In Paleopharmacology: Coprolite Evidence Of Medicinal Plants, Karl Reinhard, Donny L. Hamilton, Richard H. Hevly

Karl Reinhard Publications

Nearly 2,400 plant species have been documented ethnographically as having medicinal value among Native Americans. Therefore, it is not surprising that evidence of medicinal plants appears in some of the over 1,000 coprolites analyzed from the southwestern U.S. Three medicinal species identified in pollen analysis of coprolites are discussed. Willow (Salix) is the most common analgesic in the Native American pharmacopoeia. Prehistoric use of this plant is documented in coprolites from Bighorn Cave in the Black Mountains of Arizona and also in a burial from the Mimbres Valley, New Mexico. Historically, Mormon tea (Ephedra) served as …


Cytolytic And Ion Channel Forming Properties Of The N-Terminus Of Lymphocyte Perforin, David M. Ojcius, Pedro M. Persechini, Li-Mou Zheng, Paulo C. Notaroberto, Sandro C. Adeodato, John Ding-E. Young Jun 1991

Cytolytic And Ion Channel Forming Properties Of The N-Terminus Of Lymphocyte Perforin, David M. Ojcius, Pedro M. Persechini, Li-Mou Zheng, Paulo C. Notaroberto, Sandro C. Adeodato, John Ding-E. Young

All Dugoni School of Dentistry Faculty Articles

Perforin lyses cells by binding to the target cell membrane, where it polymerizes into large nonspecific pores. It is shown here that the first 34 amino acids of the N-terminal region of either human or murine perforin are soluble in aqueous medium and spontaneously insert into membranes. The N-terminal peptides lyse liposomes and nucleated cells, and they form ion channels in planar bilayers, some of which are comparable to those previously described for perforin. The lytic activity of the N-terminal domains does not require calcium, is independent of the lipid headgroup composition, and can be inhibited by heparin. Tumor cells …


Phyletic Coevolution Between Subterranean Rodents Of The Genus Ctenomys (Rodentia: Hystricognathi) And Nematodes Of The Genus Paraspidodera (Heterakoidea: Aspidoderidae) In The Neotropics: Temporal And Evolutionary Implications, Scott Lyell Gardner May 1991

Phyletic Coevolution Between Subterranean Rodents Of The Genus Ctenomys (Rodentia: Hystricognathi) And Nematodes Of The Genus Paraspidodera (Heterakoidea: Aspidoderidae) In The Neotropics: Temporal And Evolutionary Implications, Scott Lyell Gardner

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Coevolution was studied in six species of rodents of the genus Ctenomys and their parasitic nematodes of the genus Paraspidodera, collected in Bolivia. Representatives of the families Octodontidae and Caviidae were used as outgroups for the mammals, and nematodes from caviids were used as outgroups of the nematodes from ctenomyids. For the nematodes, quantitative and qualitative morphological characteristics of both males and females and electrophoretic characters of both sexes were used to generate phylogenetic hypotheses of evolutionary relationships of the OTUs occurring in hosts of different species. Concordance estimates of cladograms generated from biochemical-genetic and morphological data of the …


Review Of Les Nématodes Syphaciinae, Parasites De Rongeurs Et De Lagomorphes. Taxonomie, Zoogéographie, Évolution By Jean-Pierre Hugot. Zoologie, Tome 141, Mémoires Du Muséum National D'Histoire Naturelle, Paris, 1988. E. J. Brill, Leiden, The Netherlands, Robert L. Rausch Apr 1991

Review Of Les Nématodes Syphaciinae, Parasites De Rongeurs Et De Lagomorphes. Taxonomie, Zoogéographie, Évolution By Jean-Pierre Hugot. Zoologie, Tome 141, Mémoires Du Muséum National D'Histoire Naturelle, Paris, 1988. E. J. Brill, Leiden, The Netherlands, Robert L. Rausch

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Review of Les Nématodes Syphaciinae, Parasites de Rongeurs et de Lagomorphes. Taxonomie, Zoogéographie, Évolution by Jean-Pierre Hugot. Zoologie, Tome 141, Mémoires du Muséum National d'Histoire Naturelle, Paris. 1988. 148 p. + Appendix. Available from E. J. Brill, Leiden, The Netherlands.


Development And Characterization Of Monoclonal Antisperm Antibodies: Potential For Contraception, Dilrowshan H. Haque Apr 1991

Development And Characterization Of Monoclonal Antisperm Antibodies: Potential For Contraception, Dilrowshan H. Haque

Theses and Dissertations in Biomedical Sciences

There is an expressed need to develop a greater variety of safe contraceptive methods which would find acceptance worldwide, particularly in developing countries. Immunization against spermatozoa might be such a method as judged by the accumulated evidence from studies on human females and in a number of female animal species.

Two extraction techniques were used for sperm membrane antigen isolation. The first technique involved NP-40 detergent for antigen extraction from human motile sperm and the second technique employed homogenized human testis for antigen extraction. Using these immunogens, sperm membrane-specific monoclonal antibodies (MAbs) were developed. When these antisperm MAbs were subjected …


Effect Of Desiccation, Ph, Heat, And Ultraviolet Irradiation On Viability Of Baculovirus Penaei, Brian D. Leblanc, Robin M. Overstreet Mar 1991

Effect Of Desiccation, Ph, Heat, And Ultraviolet Irradiation On Viability Of Baculovirus Penaei, Brian D. Leblanc, Robin M. Overstreet

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Tolerance of Baculovirus penaei virus to desiccation, pH, heat, and ultraviolet irradiation (uv), as well as its survival in 32 ppt sea water, was investigated using larval Penaeus vannamei in a bioassay. Test shrimp were sampled from 36 to 264 hr after food-borne exposure to treated B. penaei and examined for patent infections (polyhedral inclusion bodies detectable in fresh preparations of hepatopancreas) with light microscopy. B. penaei was completely inactivated within 30 min after exposure to pH 3, whereas exposure to pH 11 extended the prepatency period (period before infection is apparent) but did not inactivate the virus. B. penaei …


Salsuginus Yutanensis N. Sp. (Monogenea: Ancyrocephalidae) From Fundulus Sciadicus In Clear Creek Of Eastern Nebraska, Michael T. Ferdig, Mary Ann Mcdowell, John J. Janovy Jr. Feb 1991

Salsuginus Yutanensis N. Sp. (Monogenea: Ancyrocephalidae) From Fundulus Sciadicus In Clear Creek Of Eastern Nebraska, Michael T. Ferdig, Mary Ann Mcdowell, John J. Janovy Jr.

John J. Janovy, Jr. Publications

Salsuginus yutanensis n. sp. (Monogenea: Ancyrocephalidae) is described from the gills of the Plains topminnow, Fundulus sciadicus Cope, from Clear Creek in eastern Nebraska. Salsuginus yutanensis is distinguished from previously described species by having a shorter accessory piece and different hamulus proportions, especially in the relative lengths of deep and superficial roots. In addition, the angles between deep and superficial roots distinguish S. yutanensis from several congeners.


Cells That Express All Five Proteins Of Vesicular Stomatitis Virus From Cloned Cdnas Support Replication, Assembly, And Budding Of Defective Interfering Particles, Asit K. Pattnaik, Gail W. Wertz Feb 1991

Cells That Express All Five Proteins Of Vesicular Stomatitis Virus From Cloned Cdnas Support Replication, Assembly, And Budding Of Defective Interfering Particles, Asit K. Pattnaik, Gail W. Wertz

School of Veterinary and Biomedical Sciences: Faculty Publications

An alternative approach to structurefunction analysis of vesicular stomatitis virus (VSV) gene products and their interactions with one another during each phase of the viral life cycle is described. We showed previously by using the vaccinia viruslT7 RNA polymerase expression system that when cells expressing the nucleocapsid protein (N), the phosphoprotein (NS), and the large polymerase protein (L) of VSV were superinfected with defective interfering (DI) particles, rapid and efflicient replication and amplification of DI particle RNA occurred. Here, we demonstrate that all five VSV proteins can be expressed simultaneously when cells are cotransfected with plasmids containing the matrix protein …


Fixing Coccidian Oocysts Is Not An Adequate Solution To The Problem Of Preserving Protozoan Type Material, Donald W. Duszynski, Scott Lyell Gardner Feb 1991

Fixing Coccidian Oocysts Is Not An Adequate Solution To The Problem Of Preserving Protozoan Type Material, Donald W. Duszynski, Scott Lyell Gardner

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Fresh (36 days old) sporulated oocysts of Eimeria nieschulzi were divided into 7 groups. Control oocysts were maintained at 23 C in 2% aqueous (w/v) K2Cr2O7. The 6 experimental groups were mixed with either Bouin's solution, 10% aqueous (v/v) buffered formalin, Karnovsky's solution, glutaraldehyde, paraformaldehyde, or 70% aqueous (v/v) ethanol (EtOH). After 115 days, oocysts from all 7 groups were examined under oil immersion to determine the effect of fixation on their structural integrity. The parameters examined were lengths and widths of oocysts and sporocysts, percent sporulation (%S), and percent crenation (%C) of oocysts …