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Flea News 50 (July 1995), Robert E. Lewis

Flea News

Obituary: Maria Suciu (January 9, 1932-September 24, 1994)

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Siphonaptera literature

Heterozygosity Mapping Of Partially Congenic Lines: Mapping Of A Semidominant Neurological Mutation, Wheels ( Whl), On Mouse Chromosome 4, Patrick M. Nolan, Patricia J. Sollars, Barbara A. Bohne, Warren J. Ewens, Gary E. Pickard, Maja Bucan

School of Veterinary and Biomedical Sciences: Faculty Publications

We identified a semidominant, chemically induced, mouse use mutation with a complex array of abnormal behaviors including bidirectional circling and hyperactivity, abnormal circadian rhythmicity and abnormal responses to light. In this report, we genetically and phenotypically characterized the circling/ waltzing component of the abnormal behavior. We mapped the locus controlling this trait by heterozygosity mapping of partially congenic lines carrying the mutagenized chromosome outcrossed to different inbred strains for three generations. Analysis of 68 PCR-based markers in 13 affected individuals indicated that the mutant locus, named Wheels (Whl), resides in the subcentromeric portion of mouse chromosome 4. The …

Three New Eimerians In Capybara (Hydrochaeris Hydrochaeris) Populations From Eastern Bolivia And Southern Venezuela, M. C. Casas, Donald W. Duszynski, L. M. Zalles

Harold W. Manter Laboratory of Parasitology: Faculty Publications

From June to August 1993, fecal samples were collected from the intestinal tract of 13 juvenile, 4 pre-adult, and 25 adult capybaras (Hydrochaeris hydrochaeris L.) from 10 localities in eastern Bolivia; additionally, 19 fresh fecal samples were collected in 1994 from capybara herds at 2 localities in south central Venezuela. Oocysts of 3 eimerians were recovered from the capybara populations in both countries and, after study, are described here as new species. Sporulated oocysts of Eimeria trinidadensis n. sp. are subspheroidal, 20.9 x 18.1 (18-23 x 16.5-21) μm with sporocysts ovoidal, 9.8 x 6.1 (8-12 x 5-8) μm. Micropyle …

Acanthobothrium Nicoyaense N. Sp. (Eucestoda: Tetraphyllidea: Onchobothriidae) In Aetobatus Narinari (Euphrasen) (Chondrichthyes: Myliobatiformes: Myliobatidae) From The Gulf Of Nicoya, Costa Rica, Daniel R. Brooks, Sharon Mccorquodale

Harold W. Manter Laboratory of Parasitology: Faculty Publications

A new species of Acanthobothrium in Aetobatus narinari from the Gulf of Nicoya, Costa Rica, most closely resembles Acanthobothrium colombianum in the same host and A. urotrygoni in Urotrygon venezuelae from Cartagena, Colombia, by having relatively few proglottides per strobila, spinose cephalic peduncles, bothridial margins free at their posterior ends, and irregularly shaped rather than spherical or elongate cirrus sacs. Acanthobothrium colombianum differs from the new species by being as much as 35 mm rather than no more than 4.7 mm long and by having 31-48 rather than 13-19 proglottides, an average of 46 rather than 15 testes per proglottis, …

Phylogenetic Relationships Of The Genera Of The Pronocephalidae Looss, 1902 (Digenea: Paramphistomiformes), Gerardo Pérez Ponce De León, Daniel R. Brooks

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Quantitative phylogenetic analysis of 20 nominal genera of the Pronocephalidae based on 47 morphological transformation series produced 6 equally parsimonious trees, each with a consistency index of 77.8%. All trees agree that Adenogaster is the sister group to the rest of the pronocephalids, and a new subfamily is proposed for it. The Pronocephalinae comprises Pronocephalus, Ruicephalus, Neopronocephalus, Macravestibulum, Choanophorus, Cetiosaccus, and Metacetabulum. The Charaxicephalinae comprises Charaxicephalus, Desmogonius, Diaschistorchis, Pleurogonius, Iguanacola, Renigonius, Parapleurogonius, Himasomum, Pyelosomum, Cricocephalus, Barisomum, and Pseudobarisomum. An amended diagnosis …

Phylogenetic Relationships Among The Species Of Pyelosomum Looss, 1899 (Digenea: Pronocephalidae), Gerardo Perez-Ponce De Leon, Daniel R. Brooks

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Comparative morphological study of 10 nominal species of pronocephalids forming a clade diagnosed by plump rounded bodies, sinuous ceca, and short esophagi supports the recognition of 7 species, with Pyelosomum longicaecum a junior synonym of P. renicapite, and P. solum and Myosaccus chelonei species incertae sedis. Phylogenetic analysis of the 7 recognized species, based on 18 morphological transformation series, produced a single tree with a consistency index of 86%. That tree suggests that Pyelosomum is paraphyletic unless Astrorchis, Epibathra, and Myosaccus are included in it. Accordingly, all are considered junior synonyms of Pyelosomum. Epibathra stenobursata is …

Evolutionary Origin Of Plasmodium And Other Apicomplexa Based On Rrna Genes, Ananias A. Escalante, Francisco J. Ayala

Harold W. Manter Laboratory of Parasitology: Library Materials

We have explored the evolutionary history of the Apicomplexa and two related protistan phyla, Dinozoa and Ciliophora, by comparing the nucleotide sequences of small subunit ribosomal RNA genes. We conclude that the Plasmodium lineage, to which the malarial parasites belong, diverged from other apicomplexan lineages (piroplasmids and coccidians) several hundred million years ago, perhaps even before the Cambrian. The Plasmodium radiation, which gave rise to several species parasitic to humans, occurred 129 million years ago; Plasmodium parasitism of humans has independently arisen several times. The origin of apicomplexans (Plasmodium), dinoflagellates, and ciliates may be >1 billion years old, …

Restoration Of Circadian Behavior By Anterior Hypothalamic Heterografts, Patricia J. Sollars, Daniel P. Kimble, Gary E. Pickard

School of Veterinary and Biomedical Sciences: Faculty Publications

The suprachiasmatic nucleus (SCN) of the anterior hypothalamus (AH) is a circadian oscillator and an important component of the mammalian circadian system. To determine whether the SCN is the dominant circadian pacemaker responsible for generating a species-typical characteristic of circadian rhythms [i.e., period length (T)], neural transplantation was conducted using fetal AH donors of different species and SCN-lesioned (SCNx) hosts. The circadian behavior of each of the three donor species is clearly distinguishable by its species-typical T. The extent of SCN pacemaker autonomy was assessed by noting whether the period of the restored circadian rhythm following heterograft transplantation was characteristic …

Morphology And Morphometrics Of Three Oxyurids Parasitic In Primates With A Description Of Lemuricola Microcebi N. Sp., Jean-Pierre Hugot, Serge Morand, Scott Lyell Gardner

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Morphology and morphometrics of three oxyurids parasitic in primates with a description of Lemuricola microcebi n. sp. Classical and morphometric analyses were conducted on several samples of oxyurid nematodes parasitic in strepsirrhine primates: Cheirogaleus major, Galago senegalensis, and Microcebus murinus. A diagnosis of Lemuricola contagiosus Chabaud & Petter, 1959 from C. major is given using syntypes, from which a lectotype is selected. The rest of the specimens were compared to L. contagiosus. From measurements taken from each sex, an initial Principal Component Analysis (PCA) was performed on log-transformed data and a second PCA was performed on …

Serendip Deborahae N. Gen. And N. Sp. (Eucestoda: Tetraphyllidea: Serendipidae N. Fam.) In Rhinoptera Steindachneri Evermann And Jenkins, 1891 (Chondrichthyes: Myliobatiformes: Myliobatidae) From Southeastern Ecuador, Daniel R. Brooks, Ramiro Barriga

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Cestodes collected in spiral valves of Rhinoptera steindachneri from the southern coast of Ecuador represent an undescribed species of Tetraphyllidea. The new species has bothridia possessing septa but lacking apical suckers. It is diagnosably distinct from all other tetraphyllidean genera by possessing a scolex comprising 4 triangular bothridia that are fused together forming a platelike structure, each of which is subdivided by 2 simple and 1 bifurcating septa radiating from its base and ringed by marginal loculi; therefore, a new genus is proposed for it. By exhibiting some degree of bothridial fusion, testes arranged in 2 layers in the proglottis …

Breeding Sheep For Worm Resistance, John Karlsson, Johan Greeff, Julia Harris

Journal of the Department of Agriculture, Western Australia, Series 4

Sheep production os one of Western Australia's most important agricultural industries. However, it is faced with the serious threat of sheep worm populations becoming increasingly resistant to the available drenches.

Although it's not a 'quick fix' solution, part of the long term answer may be selection for sheep with greater resistance to worms.

Cucumber Mosiac Virus In Lupins, Annette Bwye, Roger Jones, Wayne Proudlove

Journal of the Department of Agriculture, Western Australia, Series 4

Cucumber mosaic virus is a threat to lupin crops from Geraldton to Esperance, particularly to those growing in areas receiving more than 400 mm average annual rainfall. The disease markedly decreases grain yield in narrow-leafed and yellow lupins but doesnot infect albus or sandplain lupins. Worldwide , after bean yellow mosaic virus, cucumber mosaic virus is the second most important virus affecting lupins. Annette Bwye, Roger Jones and Wayne Proudlove outline the symptoms, spread and management of this serious disease.

Certain Canine Weakly Β-Hemolytic Intestinal Spirochetes Are Phenotypically And Genotypically Related To Spirochetes Associated With Human And Porcine Intestinal Spirochetosis, Gerald E. Duhamel, Nagaraja Muniappa, Michelle R. Mathiesen, J. L. Johnson, J. Toth, R. O. Elder, A. R. Doster

School of Veterinary and Biomedical Sciences: Faculty Publications

Four canine weakly β-hemolytic intestinal spirochetes associated with intestinal spirochetosis (IS-associated WBHIS) were compared with IS-associated human and porcine WBHIS and the type species for Serpulina hyodysenteriae and S. innocens by using phenotypic and genotypic parameters. The IS-associated canine, human, and porcine WBHIS belonged to a phyletic group distinct from but related to previously described Serpulina type species.

Genetic Structure Of Midwestern Ascaris Suum Populations: A Comparison Of Isoenzyme And Rapd Markers, Steven A. Nadler, Rachel L. Lindquist, Thomas J. Near

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Isoenzyme and random amplified polymorphic DNA (RAPD) markers were used to characterize the genetics of geographic variation among population samples of Ascaris suum from midwestern localities. Independent estimates of fixation indices (F_ST) based on isoenzyme and RAPD markers showed the same general patterns of differentiation and substantial statistical correlation (r=0.70). Of the total estimated gene diversity, 9.4% (isoenzyme) and 9.2% (RAPD) was distributed among infrapopulations. Geographic localities accounted for 7.8% (isoenzyme) and 6.2% (RAPD) of the total gene diversity. Only infrapopulations and localities, which indicates significant population subdivision among A. suum from farms within geographic regions. Departures from …

Microevolution And The Genetic Structure Of Parasite Populations, Steven A. Nadler

Harold W. Manter Laboratory of Parasitology: Faculty Publications

The development of polymerase chain reaction-based methods for assessing the genotypes of small individual organisms will promote groundbreaking investigations of the genetic architecture of parasite populations. Both quantitative genetic models and general knowledge of parasite natural history are useful for making general predictions about the distribution of genetic variation over geographic space. However, designing experimental studies to assess relationships between specific life history variables and patterns of genetic structure in natural populations will be challenging. Traditional biochemical-genetic methods have already been used to study a limited number of parasite populations, and inferred patterns of genetic structure are distinctly different between …

Critical Comments: Parasitology Year 2000, Albert O. Bush, Janine N. Caira, Dennis J. Minchella, Steven A. Nadler, John R. Seed

Harold W. Manter Laboratory of Parasitology: Faculty Publications

We predict that in order for parasitology to thrive by the year 2000 the various subdisciplines of evolution, ecology, biosystematics, and genetics must develop holistic approaches and use parasite models to answer basic biological questions. The students of tomorrow must work as part of a multidisciplinary team; and their questions and answers must be conceptually integrated into the broader biological framework of evolution and ecology.

Comparative Morphology Of The Scolices And Microtriches Among Five Species Of Tetrabothrius (Eucestoda: Tetrabothriidae), Eric P. Hoberg, D. E. Sims, P. H. Odense

Harold W. Manter Laboratory of Parasitology: Faculty Publications

The structure of the scolex, neck, and adjacent strobila of 5 species of Tetrabothrius was evaluated using low to high magnification scanning electron miscroscopy. Species-specific patterns and morphotypes (spiniform, papilliform, and filiform) of microtriches were found on scolices and strobilar tegument of Tetrabothrius (Oriana) affinis, Tetrabothrius (Oriana) filiformis, and Tetrabothrius (Tetrabothrius) sp. The presence, distribution, and form of microtriches may not be correlated with the subgeneric divisions of Tetrabothrius or the host taxon. Unique patterns do not appear to be shared with other groups of Cestodes, particularly other tetraphyllideans. We suggest that the spiniform, filiform, and papilliform types of microtriches …

Libyostrongylus Dentatus N. Sp. (Nematoda: Trichostrongylidae) From Ostriches In North America, With Comments On The Genera Libyostrongylus And Paralibyostrongylus, Eric P. Hoberg, S. Lloyd, H. Omar

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Libyostrongylus dentatus sp. n. is described from ostriches on farms from North Carolina and Texas. Nematodes were recovered from the posterior proventriculus and under the koilon lining of the gizzard; the parasites occurred in mixed infections with Libyostrongylus douglassii. The species is distinguished from congeners by the presence of a prominent, dorsal, esophageal tooth; in males by the structure of the dorsal ray and spicules; and in females by small eggs (52-62 gm in length), a sublateral vulva situated at 93% of the body length from the anterior, and a strongly curled, digitate, tail with cuticular inflations at the …

Silver Staining For Elucidation Of The Synlophe In Trichostrongyle Nematodes, Alexander V. Khrustalev, Eric P. Hoberg

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Staining techniques are relatively rare in the study of parasitic nematodes. A novel silver-staining method is described for elucidation of the synlophe (a system of longitudinal cuticular ridges), a character of great systematic importance among the trichostrongyloid nematodes. Ridges are stained optically black and appear in great contrast to the body of the nematode. This method augments current use of interference contrast for examination of the synlophe. Detailed studies of the configuration of the synlophe in entire specimens are possible with standard light microscopy for the first time.

Historical Biogeography And Modes Of Speciation Across High-Latitude Seas Of The Holarctic: Concepts For Host-Parasite Coevolution Among The Phocini (Phocidae) And Tetrabothriidae (Eucestoda), Eric P. Hoberg

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Species of Anophryocephalus are host-specific parasites of pinnipeds in the Holarctic. Phylogenetic analysis of 7 species postulates A. anophrys as the basal taxon and A. inuitorum as basal to A. skrjabini; A. arcticensis is basal to A. nunivakensis and A. eumetopii + A. ochotensis (single tree; consistency index = 74.4%; homoplasy slope ratio = 36.45%). Evaluation of host and geographic distributions postulates ringed seals of the Atlantic-Arctic as ancestral hosts, and the Arctic basin as a paraphyletic area with respect to the North Pacific. Cospeciation within this assemblage was dependent on intense isolation of small effective populations of definitive hosts …

Anophryocephalus Inuitorum Sp. Nov. And A. Arcticensis Sp. Nov. (Eucestoda: Tetrabothriidae) In Ringed Seals (Phoca Hispida Hispida) And Harp Seals (Phoca Groenlandica) From High-Latitude Seas Of Eastern Canada And The Arctic Basin, Eric P. Hoberg, Lena N. Measures

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Anophryocephalus inuitorum sp. nov. and A. arcticensis sp. nov. are described from ringed seals (Phoca hispida hispida) in the eastern Canadian Arctic; the latter species is also reported from harp seals (Phoca groenlandica) in the Gulf of St. Lawrence. Anophryocephalus inuitorum is most similar to A. skrjabini, but can be distinguished by fewer testes (14-27) and smaller dimensions of the strobila, neck (3.0-5.9 mm long), and cirrus sac (31-70 μm long), diameter of the genital atrium (44 -68 pm), and length of the male canal (23-42 μm long). Anophyrocephulus arcticensis resembles A. nunivakensis in the …

Cluster Analysis Of The Coprolites From Antelope House: Implications For Anasazi Diet And Cuisine, Mark Q. Sutton, Karl Reinhard

Karl Reinhard Publications

This paper reports on a cluster analysis of 155 coprolites from Antelope House, a prehistoric Anasazi site in Canyon de Chelly, Arizona. The analysis revealed three primary clusters; whole kernel maize, milled maize, and nonmaize, which we believe to represent seasonal- and preference-related cuisine. Protein residue analysis on a subsample of the specimens added depth to the analysis.

A New Pinworm, Didelphoxyuris Thylamisis N. Gen., N. Sp. (Nematoda: Oxyurida) From Thylamys Elegans (Waterhouse, 1839) (Marsupialia: Didelphidae) In Bolivia, Scott Lyell Gardner, Jean-Pierre Hugot

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Didelphoxyuris thylamisis n. gen., n. sp. is described from the caecum of Thylamys elegans (Waterhouse, 1839) (Marsupialia: Didelphidae) collected in the eastern region of the Andes of Bolivia. Didelphoxyuris thylamisis n. sp. differs from the only pinworm described from marsupials in the Neotropics (Neohilgertia venusti Navone, Suriano et Pujol, 1990) in having only three oesophageal teeth, non-operculated eggs, females that are didelphic, and males that possess no preanal papillae. Several other species of pinworms have been described from marsupials in Australia, but all are characterized by possessing a buccal capsule that is strongly cuticularized with inter-radial lamellae. These structures …

Caryospora Simplex (Apicomplexa: Eimeriidae) From A Captive Kaznakov's Viper (Vipera Kaznakovi), Patricia G. Wilber, John A. Hnida, Michael J. Richard, Donald W. Duszynski

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Caryospora simplex is reported for the first time from the feces of a captive female Kaznakov’s viper (Vipera kaznakovi) in Albuquerque, New Mexico (USA). Coccidian meronts and gamonts were observed in the intestinal epithelial cells of another female Kaznakov’s viper that died in October 1993.

Caridean Shrimps Associated With The Slimy Sea Plume (Pseudopterogorigia Americana) In Midsummer At Guana Island, British Virgin Islands, West Indies, Stephen Spotte, Patricia M. Bubucis, Robin M. Overstreet

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Fifty-one slimy sea plumes (Pseudopterogorgia americana Gmelin, 1791) were sampled for caridean shrimps at Guana Island, British Virgin Islands, during one week in July 1992. Sam- pling depth ranged from 3-22 m. Nine species were collected: Hippolyte nicholsoni Chace, 1972; Latreutes sp.; Neopontonides chacei Heard, 1986; Perclimenes cf. patae Heard and Spotte, 1991; Periclimenes cf. pauper Holthuis, 1951; Periclimenes sp.; Pseudocoutierea antillensis Chace, 1972; Tozeuma cf. cornutum Milne Edwards, 1881; and Trachycaris rugosa (Bate, 1888). A total of 1,418 specimens (including fragments) was obtained. The number of shrimp species per gorgonian ranged from 1-5; one gorgonian harbored 156 shrimps. …

Umingmakstrongylus Pallikuukensis Gen. Nov. Et Sp. Nov. (Nematoda: Protostrongylidae) From Muskoxen, Ovibos Moschafus, In The Central Canadian Arctic, With Comments On Biology And Biogeography, Eric P. Hoberg, Lydden Polley, A. Gunn, J. S. Nishi

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Umingmakstrongylus pallikuukensis gen. nov. et sp. nov. is established for a protostrongylid nematode in muskoxen, Ovibos moschatus, from the Kitikmeot Region (central Arctic) of the Northwest Territories, Canada. It is distinguished from Cystocaulus and other Muelleriinae by characters that include the following: males: deeply incised, bilobed bursa, independent externodorsal rays, telamon composed of distal transverse plate, absence of falcate crurae, and spicules not distally split; females: absence of provagina; and first-stage larvae: presence of three cuticular folds on the tail. The great length of females (468 mm) and males (171 mm) is exceptional among the Protostrongylidae. Pathognomonic lesions include …

Phase Ii Trial Of Murine Monoclonal Antibody D612 Combined With Recombinant Human Monocyte Colony-Stimulating Factor (Rhm-Csf) In Patients With Metastatic Gastrointestinal Cancer, Mansoor Saleh, Michael Khazaeli, Richard Wheeler, Pat Bucy, Tiepe Liu, Michael Everson, David Mun, Jeff Schlom

Haematology and Oncology, East Africa

In a Phase II study, 14 patients with metastatic gastrointestinal cancer received the mAb D612 (40 mg/m2, days 4, 7, and 11) in combination with recombinant human monocyte colony-stimulating factor [(rhM-CSF) 80 ug/kg/days 1-14]. The combined treatment was well tolerated and resulted in characteristic biological activity associated with each of the agents. Thus, 10 of 14 patients experienced D612-associated secretory diarrhea, which responded to the prostaglandin inhibitor Indomethacin in 5 of 7 patients. rhM-CSF therapy was associated with peripheral mono- cytosis (peak absolute monocyte count, 1444 ±394/mm3) and thrombocytopenia (nadir count, 78 ±10/nim '). Monocyte surface marker analysis revealed a …

Identifying The Processed Products Of The Polymerase Polyprotein Of Coronavirus, Hong-Qiang Gao

Dissertations

No abstract provided.

A Survey Of Source And Finished Water Supplies For Giardia Cysts And Cryptosporidium Oocysts, Lisa Martin Sexton

KWRRI Research Reports

The occurrence of Giardia lamblia cysts and Cryptosporidium parvum oocysts was determined from source and treated drinking water supplies at selected water treatment facilities in Eastern Kentucky. Giardia and Cryptosporidium are two human protozoan parasites often found in water supplies due to fecal contamination. Characteristic cysts and oocysts were identified from large volume water samples, typically 100 gallons for raw samples and 1,000 gallons for finished water, using an indirect fluorescent antibody specific for Giardia and Cryptosporidium. Source water from four different water treatment facilities utilizing a reservoir, river, and two creeks were assayed. All sites exhibited Giardia lamblia …