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Effect Of Bromelain On Duck Breast Meat Tenderization, Ahmet Buyukyavuz May 2014

Effect Of Bromelain On Duck Breast Meat Tenderization, Ahmet Buyukyavuz

All Theses

ABSTRACT This study was conducted to determine the effect of bromelain on duck breast meat tenderization. Duck breasts were marinated with different concentrations (0, 1.5, 3 and 4.5%; C2, B1.5, B3 and B4.5, respectively) of bromelain and a solution composed of 92% refrigerated water, 6% salt and 2% sodium tripolyphosphate by using a vacuum food tumbler machine. A second non-marinated control (C1) was also included. Marination tumbling was operated at 8 RPM for 8 minutes. Physico-chemical and quality parameters were determined on raw and cooked samples. A significant reduction (p<0.05) in pH was observed in all bromelain treated samples when compared to C2. Although there was a significant reduction in pH of B1.5 when compared to C1, there was no significant difference among B3, B4.5 and C1. A significant increase was observed in water holding capacity of B3 and B4.5 when compared to C1. While a significant reduction was observed in lightness (L*), no differences were observed in redness (a*) and yellowness (b*) values of marinated samples (C2, B1.5, B3 and B4.5) when compared to C1. However, a significant increase in lightness (L*), and a significant decrease in redness (a*) and yellowness (b*) were observed in cooked bromelain treated samples. No differences were observed in razor shear forces, moisture content and water activity between treatments. There was no significant difference in cook yield of all marinated samples (C2, B1.5, B3, and B4) when compared to the C1. A significant reduction (p<0.05) in Warner-Bratzler shear force values was observed in all marinated samples when compared to untreated control (C1). Based on results, use of bromelain in marination solutions under the conditions tested here would not be cost effective.


Comparison Of Simultaneous Distillation And Extraction (Sde) And Headspace Solid Phase Microextraction (Spme) For Determination Of Volatiles Of Muscadine Grapes (Vitis Rotundifolia), Pei-Ching Lin May 2014

Comparison Of Simultaneous Distillation And Extraction (Sde) And Headspace Solid Phase Microextraction (Spme) For Determination Of Volatiles Of Muscadine Grapes (Vitis Rotundifolia), Pei-Ching Lin

All Theses

Vitis rotundifolia also called 'Muscadine' grows abundantly throughout the southeastern United States. Muscadine grapes have desirable flavors, making them suitable to be either consumed as fresh fruit or made into wine and jam. Moreover, muscadine grapes have been reported containing rich complex phytochemicals, including anthocyanins and polyphenols that possess antioxidant, anti-cancer, anti-inflammatory and antimicrobial activities. Therefore, muscadine grapes have become increasingly popular in recent years due to its unique aroma, as well as health benefits. Various volatile compounds contribute to the aromatic flavors of muscadine grapes. In regards of their low concentrations, conventional simultaneous distillation and extraction (SDE) and solid …


Investigation Of Rice Bran Derived Anti-Cancer Pentapeptide For Mechanistic Potency In Breast Cancer Cell Models, Ruiqi Li May 2014

Investigation Of Rice Bran Derived Anti-Cancer Pentapeptide For Mechanistic Potency In Breast Cancer Cell Models, Ruiqi Li

Graduate Theses and Dissertations

Bioactive peptides derived from food sources with anti-proliferative properties against cancer have drawn more attention in recent years. A pentapeptide derived from rice bran has shown anti-proliferative propertiesagainst human breast cancer cells. The objective of this study was to investigate the mechanistic action of the pentapeptide-induced apoptosis in breast cancer cell models (MCF-7 and MDA-MB-231). The growth inhibition activity of the pentapeptide was

evaluated by MTS[3-(4,5-dimethylthiazol-2-yl)-5-(3- arboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, inner salt] assayand trypan blue assay in a dose- and time-dependent manner.

The apoptotic properties of pentapeptide-induced apoptosis on cancerous breast cells were evaluated by morphological changes, DNA fragmentation, and caspases-3/7, -8,and -9 …


Effect Of Limit-Fed Co-Product Feedstuffs On Production, Digestion, Fermentation And Rumen Function In Beef Cattle, William Brandon Smith May 2014

Effect Of Limit-Fed Co-Product Feedstuffs On Production, Digestion, Fermentation And Rumen Function In Beef Cattle, William Brandon Smith

Graduate Theses and Dissertations

In terms of energy density, the cost of shipping hay is often not justified in yr where adverse conditions limit available forage. Our objective was to determine if co-product feedstuffs could be used to meet the energy demands for cows in late pregnancy. Eighty-six crossbred cows (527 ± 0.8 kg BW) in late gestation were stratified by BW, BCS and age and allocated randomly to 1 of 6 groups held on 2-ha dormant bermudagrass pastures for 68 d. Three groups were offered bermudagrass hay ad libitum (HAY) and three groups were offered 6.4 kg of soybean hulls (LSH) daily and …


Effects Of Processing And Packaging Modifications On The Quality And Shelf Life In Meats, Abdulla Khan May 2014

Effects Of Processing And Packaging Modifications On The Quality And Shelf Life In Meats, Abdulla Khan

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Shelf life of foods, mainly meat and meat products, is affected by its physical and chemical properties like color, pH, water holding capacity etc. In developed countries
food losses occur at production, retail and consumer levels with meat commodities accounting for 41% of the total loss in US during 2008. These losses are because of
spoilage due to inappropriate packaging, improper storage conditions, food wastage, and lack of consumer awareness. By focusing on shelf life and quality issues at the production level, we can overcome some of the major problems faced by the food manufacturers.

Active packaging is an innovative …


The Triad Trial: Online Education For Coaches On The Prevention Of The Female Athlete Triad, Megan Jane Ostler May 2014

The Triad Trial: Online Education For Coaches On The Prevention Of The Female Athlete Triad, Megan Jane Ostler

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

High school coaches play a huge role in establishing a healthy environment for their athletes and often students. The coach‐athlete relationship has proven to be a strong and useful bond in prevention of adverse behaviors of athletes or in prevention of health problems such as concussions. The female athlete triad (triad) is a common health problem among female athletes. Many high school coaches are unaware of the triad or the serious health and performance consequences for their athletes. The triad is a syndrome marked by 3 interrelated adverse effects: decreased energy availability, menstrual dysfunction, and decreased bone mineral density.

The …


Nutrition And Genes Associated With Orofacial Cleft Birth Defects In Utah, Huong Dieu Meeks May 2014

Nutrition And Genes Associated With Orofacial Cleft Birth Defects In Utah, Huong Dieu Meeks

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Orofacial clefts (OFCs) are facial malformations that happen during early pregnancy and have a complex and heterogeneous etiology, involving both genetic and environmental risk factors. This project examined the association between maternal nutrition, folate-related biomarkers, candidate genes involved in one-carbon metabolism (OCM), and OFCs in order to achieve more comprehensive knowledge of how nutrition and genetics influence OFC risk. All studies performed in this project used the data collected in the Utah Child and Family Health Study (UCFHS) and the Johns Hopkins International Cleft Consortium.

Neither maternal periconceptional multivitamin use nor healthy dietary pattern score alone was individually associated with …


Precautionary Labelling Of Foods For Allergen Content: Are We Ready For A Global Framework?, Katrina J. Allen, Paul J. Turner, Ruby Pawankar, Steve Taylor, Scott Sicherer, Gideon Lack, Nelson Rosario, Motohiro Ebisawa, Gary Wong, E. N. Clare Mills, Kirsten Beyer, Alessandro Fiocchi, Hugh A. Sampson Apr 2014

Precautionary Labelling Of Foods For Allergen Content: Are We Ready For A Global Framework?, Katrina J. Allen, Paul J. Turner, Ruby Pawankar, Steve Taylor, Scott Sicherer, Gideon Lack, Nelson Rosario, Motohiro Ebisawa, Gary Wong, E. N. Clare Mills, Kirsten Beyer, Alessandro Fiocchi, Hugh A. Sampson

Department of Food Science and Technology: Faculty Publications

Food allergy appears to be on the rise with the current mainstay of treatment centred on allergen avoidance. Mandatory allergen labelling has improved the safety of food for allergic consumers. However an additional form of voluntary labelling (termed precautionary allergen labelling) has evolved on a wide range of packaged goods, in a bid by manufacturers to minimise risk to customers, and the negative impact on business that might result from exposure to trace amounts of food allergen present during cross-contamination during production. This has resulted in near ubiquitous utilisation of a multitude of different precautionary allergen labels with subsequent confusion …


Tpn Collaborative: Improving Network Wide Parenteral Nutrition Care, Christina Christianson Rd, Cnsc, Ldn, Anthony Buonanno Md, Mba Apr 2014

Tpn Collaborative: Improving Network Wide Parenteral Nutrition Care, Christina Christianson Rd, Cnsc, Ldn, Anthony Buonanno Md, Mba

Clinical Nutrition Service

No abstract provided.


No Patient Left Behind, Courtney Bloom Rd, Ldn, Melissa Faura Rd, Ldn, Ann Flickinger Ms, Rd, Ldn, Karen A. Yerger Rd, Ldn Apr 2014

No Patient Left Behind, Courtney Bloom Rd, Ldn, Melissa Faura Rd, Ldn, Ann Flickinger Ms, Rd, Ldn, Karen A. Yerger Rd, Ldn

Clinical Nutrition Service

No abstract provided.


Teach-Back For Inpatient Nutrition Education: Mission Possible, Melissa Faura Rd, Ldn, Ann Flickinger Ms, Rd, Ldn, Katherine Lyle Ms, Rd, Ldn Apr 2014

Teach-Back For Inpatient Nutrition Education: Mission Possible, Melissa Faura Rd, Ldn, Ann Flickinger Ms, Rd, Ldn, Katherine Lyle Ms, Rd, Ldn

Clinical Nutrition Service

No abstract provided.


Calculating Value- Added Services Of The Registered Dietitian, Melissa Faura Rd, Ldn, Ann Flickinger Ms, Rd, Ldn, Kathleen Hanuschak Rd, Ldn, Krysta Miller Rd, Ldn, Renee Selby Rd, Cnsc, Ldn Apr 2014

Calculating Value- Added Services Of The Registered Dietitian, Melissa Faura Rd, Ldn, Ann Flickinger Ms, Rd, Ldn, Kathleen Hanuschak Rd, Ldn, Krysta Miller Rd, Ldn, Renee Selby Rd, Cnsc, Ldn

Clinical Nutrition Service

No abstract provided.


Characterization Of Extraction Methods To Recover Phenolic Rich Extracts From Pinto Beans That Exert High Antioxidative Activities Using Response Surface Approach, Mohammed Aldawsari Apr 2014

Characterization Of Extraction Methods To Recover Phenolic Rich Extracts From Pinto Beans That Exert High Antioxidative Activities Using Response Surface Approach, Mohammed Aldawsari

Department of Food Science and Technology: Dissertations, Theses, and Student Research

The pinto bean has been linked to the prevention of multiple diseases due in large part to the presence of phenolic antioxidants, which are higher in beans than in many fruits and vegetables. These components deliver health properties beyond basic nutritional characteristics by scavenging free oxygen radicals. However, these benefits are most likely due to the ability of these chemically diverse phenols to impart greater protective properties as additives or synergists acting in combination. However, optimal parameters to isolate these compounds (in terms of ratios and types) from a given natural source are not known. Without this knowledge, understanding the …


Case Study Of A Food Safety/Good Agricultural Practices (Gaps) Educational Program For Small And Limited Resource Produce Farmers, Barrett Vaughan, Audrey Zeigler, Gertrude D. Wall, Miles D. Robinson, William A. Hodge, Conrad O. Bonsi, Ntam R. Baharanyi, Walter A. Hill Apr 2014

Case Study Of A Food Safety/Good Agricultural Practices (Gaps) Educational Program For Small And Limited Resource Produce Farmers, Barrett Vaughan, Audrey Zeigler, Gertrude D. Wall, Miles D. Robinson, William A. Hodge, Conrad O. Bonsi, Ntam R. Baharanyi, Walter A. Hill

Professional Agricultural Workers Journal

This case study examined methods used in a food safety/Good Agricultural Practices (GAPs) educational program with small and limited resource produce farmers in Alabama to assist them with obtaining certification. Two methods were used, namely, the identification of challenges to food safety certification and development of strategies to address the challenges, and the enlistment of educational methods to facilitate food safety certification. As a result, there were four challenges to food safety certification identified; needs for motivation, information, clarification, and resources. In addition, the educational methods enlisted included group meetings, instructional material distribution, individual farm instruction, and expert instruction. The …


Developing Strategies To Mitigate And Control Environmental Listeria Monocytogenes In Retail Delis, Susan Rose Hammons Apr 2014

Developing Strategies To Mitigate And Control Environmental Listeria Monocytogenes In Retail Delis, Susan Rose Hammons

Open Access Theses

There are many research avenues being investigated at institutions nationwide with the intent to reduce the public health risk of listeriosis. Approaches include studies on L. monocytogenes transmission dynamics in retail delis, observations of deli worker practices, a structured expert elicitation on transmission dynamics and statistical modeling of listeriosis risk impact factors. This thesis describes my contribution to the work being done in our group to (i) understand prevalence and persistence of L. monocytogenes and Listeria species non-monocytogenes on environmental surfaces (food contact and non-food contact); (ii) identify characteristics or practices which place stores at increased risk for L. …


Anticipatory And Reactive Responses To Chocolate Restriction In Frequent Chocolate Consumers, Chelsey L. Keeler Apr 2014

Anticipatory And Reactive Responses To Chocolate Restriction In Frequent Chocolate Consumers, Chelsey L. Keeler

Open Access Theses

Many individuals have difficulty adhering to a weight loss diet. One possible explanation could be that dietary restriction paradoxically contributes to overconsumption. The objective of this study was to examine ingestive behavior under a forced chocolate restriction, with a focus on the anticipatory restriction period and the post-restriction period in frequent chocolate consumers. Fifty-six male (N=18) and female (N=38) high chocolate consumers with high or low cognitive disinhibition aged 27.70 ± 11.09 years with a mean BMI of 25.68± 5.92 kg/m2 participated. Chocolate snacks were provided for the first, second, and sixth week of the study to establish baseline, pre-restriction …


Fabrication And Analysis Of Soy Flour Filled Polyethylene Fibers, Ozgun Ozdemir, Sam Lukubira, Amod Ogale, Paul Dawson Apr 2014

Fabrication And Analysis Of Soy Flour Filled Polyethylene Fibers, Ozgun Ozdemir, Sam Lukubira, Amod Ogale, Paul Dawson

Graduate Research and Discovery Symposium (GRADS)

Fibers composed of soy flour (soy) and linear low density polyethylene (PE) were produced by melt spinning method. The mixing time of soy flour and PE was adjusted to get better dispersion of soy flour inside the matrix. The inclusion of soy decreased tensile modulus by 35% and tensile strength by 30% of the fibers compared with 950 MPa and 43 MPa, respectively, for PE fibers. Strain to failure for soy-PE fibers was 292% where PE fibers have strain to failure at 513%. Tensile properties of soy-PE fibers are comparable to those of pure PE. Even washing did not deteriorated …


Translational Nutrition: A Review Of The Application Of Nutrition Science, Regulation, And Outreach Case Study: Farm Fresh Rhode Island, Eliza Zalis Apr 2014

Translational Nutrition: A Review Of The Application Of Nutrition Science, Regulation, And Outreach Case Study: Farm Fresh Rhode Island, Eliza Zalis

Public & Community Service Studies Student Scholarship

This project examines the avenues by which nutrition science impacts individuals in society. It examines the factors that influence consumer behavior and food choice and the importance of health literacy in guiding nutrition and health. It elucidates the role of the Food and Drug Administration (FDA) in regulating food labeling and the implications that such regulation has on consumer perception and possible health outcomes. Further, this project outlines recently proposed changes to the “Nutrition Facts” panel. In investigating nutrition education and outreach programs, this project includes a case study of Farm Fresh Rhode Island and the organization’s nutrition initiatives.


Fatty Acids And Mercury In Seventy Seven Species Of Commercially Available Finfish In The United States, Dennis P. Cladis Apr 2014

Fatty Acids And Mercury In Seventy Seven Species Of Commercially Available Finfish In The United States, Dennis P. Cladis

Open Access Theses

Finfish are consumed across the United States and constitute an important part of the American diet. However, seafood consumption can be a tenuous topic, with supporters highlighting the health benefits of eicosapentaenoic acid (EPA, C20:5n3) and docosahexaenoic acid (DHA, C22:6n3) and opponents emphasizing the neurotoxicity of methylmercury. Because all fish contain varying amounts of EPA, DHA, and methylmercury, the need for clear and unbiased information is essential to alleviate the confusion experienced by many consumers and empower them to make informed decisions regarding seafood consumption. As the market changes and more fish originate from aquaculture sources, where diets are controlled, …


Baltimore And The Cherry Hill Urban Garden: Tearing Down And Building Up The Physical And Imaginative Spaces Of Post-Industrial Urban Food Systems, Rebecca L. Croog Apr 2014

Baltimore And The Cherry Hill Urban Garden: Tearing Down And Building Up The Physical And Imaginative Spaces Of Post-Industrial Urban Food Systems, Rebecca L. Croog

Student Publications

The tide is changing in food research and food movements. Both academic thought and grassroots mobilization have demonstrated a shift beyond merely the problems of industrial food, and toward an emphasis on issues of justice and equity within food systems (Sloccum, 2006; Alkon & Agyeman, 2011; Sbicca, 2012; Agyeman & McEntee, 2013). In examining the contemporary case of the Farm Alliance of Baltimore City, which is “a network of producers working to increase the viability of urban farming and improve access to urban grown foods, united by practices and principles that are socially, economically, and environmentally just” (Farm Alliance website, …


Shrinkage Prediction During Pasta Dehydration, Gina Michelle Bressani Apr 2014

Shrinkage Prediction During Pasta Dehydration, Gina Michelle Bressani

Open Access Theses

Shrinkage models from different fields of study were reviewed. Pasta was used as a model to further develop the hypothesis of predicting shrinkage in terms of the rate of relaxation and the rate of moisture loss. An extended literature review of pasta properties, such as isotherms, diffusivity, glass transition, rheological properties, and shrinkage was conducted. Isotherm and glass transition models were developed from the literature data acquired. Creep was tested with a 25 mm-diameter flat plate at 20 to 40°C at varying moisture content. Viscoelastic properties for the Burgers model for creep were determined, where higher moisture content samples resulted …


Study Of The Sustainbility Issues Of Food Production Using Vertical Farm Methods In An Urban Environment Within The State Of Indiana, Victor Mendez Perez Apr 2014

Study Of The Sustainbility Issues Of Food Production Using Vertical Farm Methods In An Urban Environment Within The State Of Indiana, Victor Mendez Perez

Open Access Theses

There are several problems around the globe related to water scarcity, energy shortages, deforestation and loss of fertile land, and food security. Climate change is making environmental and social issues arise. Issues such as, changes in the agricultural landscape, increasing population (9 billion is the expected population for 2040), rising food borne illnesses, scarcity of drinking water, and more crop failure due to plant pathogens and insect pests, among other factors make this study specially significant. Vertical farming could be a potencial solution to some of these problems.

Due to these problems the present thesis analyzed vertical farming as a …


Quality Changes In Hermetically Stored Corn Caused By Fungi And Sitophilus Zeamais, Nda-Agyima Addae-Mensah K Apr 2014

Quality Changes In Hermetically Stored Corn Caused By Fungi And Sitophilus Zeamais, Nda-Agyima Addae-Mensah K

Open Access Theses

Hermetic storage has been shown to be effective in controlling insect pests and maintaining grain quality of dry grains at 13% moisture and below. However, the feasibility and use of hermetic storage for grains at intermediate mid-moisture levels under the influence of the sub-Saharan African weather conditions is relatively unknown. Hermetic storage experiments were conducted on grade 1 "6297 and 6333 VT RIB" hybrid corn under controlled temperature conditions at 10 degrees centigrade and 25 degrees centigrade at target approximate moisture content levels of 11, 15, 18 and 21% wet basis for a total storage period of 6 months. Corn …


Debaryomyces Hansenii: A Foodborne Yeast That Produces Anti- Candida Killer Toxin, Nabaraj Banjara Apr 2014

Debaryomyces Hansenii: A Foodborne Yeast That Produces Anti- Candida Killer Toxin, Nabaraj Banjara

Department of Food Science and Technology: Dissertations, Theses, and Student Research

Candida yeasts are commensal members of the gastrointestinal, mucosal, oral and vaginal microbiota. Candida albicans and C. tropicalis can be found as a part of the normal human commensal flora, especially in all sections of the gastrointestinal tract. However, when the host defense system and microbiota are disturbed, Candida can become pathogenic and cause severe infection or candidiasis. Antifungal drugs used to treat candidiasis have been shown to result in treatment failures due to drug toxicity and/or development of resistance during long term antifungal therapy and, in recent years, the incidence of Candida infections has increased dramatically due to the …


Increased Dietary Docosahexaenoic Acid Consumption And Its Effect On Macular Pigment Optical Density: The Eye Health In College-Age Students Iii Pilot Study, Markita M. Lewis Apr 2014

Increased Dietary Docosahexaenoic Acid Consumption And Its Effect On Macular Pigment Optical Density: The Eye Health In College-Age Students Iii Pilot Study, Markita M. Lewis

Honors Capstones

No abstract provided.


Growing South Dakota (Spring 2014), College Of Agriculture &. Biological Sciences Apr 2014

Growing South Dakota (Spring 2014), College Of Agriculture &. Biological Sciences

Growing South Dakota (Publication of the College of Agriculture, Food and Environmental Sciences)

This issue contains the SDSU Extension 2013 Annual Report.

[Page] 2 The New SDSU Extension: Two Years Later, Change Has Brought New Successes
[Page] 4 Your 24-7 Connection: iGrow.org Makes SDSU Extension Easily Accessible, Responsive
[Page] 5 SDSU Regional Extension Centers: Your Front Door To SDSU
[Pages] 6-7 Leading The Challenge: Projects To Address Food Security, Financial Knowledge Underway
[Pages] 8-9 Regional Collaboration: SET Grants & Food Networks Initiated
[Pages] 10-11 Encouraging & Empowering: Native American Program Assists Reservations In Striving For Healthy Food, Healthy Communities & Youth Science Programming
[Page] 12 Evaluating Yields: Crop Performance Testing Provides Important, Unbiased …


Molecular Evolution Of Glycoside Hydrolase Genes In The Western Corn Rootworm (Diabrotica Virgifera Virgifera), Seong-Il Eyun, Haichuan Wang, Yannick Pauchet, Richard H. Ffrench-Constant, Andrew K. Benson, Arnubio Valencia-Jimenez, Etsuko N. Moriyama, Blair Siegfried Mar 2014

Molecular Evolution Of Glycoside Hydrolase Genes In The Western Corn Rootworm (Diabrotica Virgifera Virgifera), Seong-Il Eyun, Haichuan Wang, Yannick Pauchet, Richard H. Ffrench-Constant, Andrew K. Benson, Arnubio Valencia-Jimenez, Etsuko N. Moriyama, Blair Siegfried

Department of Entomology: Faculty Publications

Cellulose is an important nutritional resource for a number of insect herbivores. Digestion of cellulose and other polysaccharides in plant-based diets requires several types of enzymes including a number of glycoside hydrolase (GH) families. In a previous study, we showed that a single GH45 gene is present in the midgut tissue of the western corn rootworm, Diabrotica virgifera virgifera (Coleoptera: Chrysomelidae). However, the presence of multiple enzymes was also suggested by the lack of a significant biological response when the expression of the gene was silenced by RNA interference. In order to clarify the repertoire of cellulose-degrading enzymes and related …


Dietary Antiaging Phytochemicals And Mechanisms Associated With Prolonged Survival, Hongwei Si, Dongmin Liu Mar 2014

Dietary Antiaging Phytochemicals And Mechanisms Associated With Prolonged Survival, Hongwei Si, Dongmin Liu

Human Sciences Faculty Research

Aging is well-known an inevitable process that is influenced by genetic, lifestyle and environmental factors. However, the exact mechanisms underlying the aging process are not well understood. Increasing evidence shows that aging is highly associated with chronic increase in reactive oxygen species (ROS), accumulation of a low-grade proinflammatory phenotype and reduction in age-related autophagy, suggesting that these factors may play important roles in promoting aging. Indeed, reduction of ROS and low-grade inflammation and promotion of autophagy by calorie restriction or other dietary manipulation can extend lifespan in a wide spectrum of model organisms. Interestingly, recent studies show that some food-derived …


The Relationship Of Nd1 Mtdna Gene Polymorphisms To Energy Metabolism And Risk Of Obesity Among Peruvian Native Americans, Karen K. Thomas, Dr. Mark J. Rowe Feb 2014

The Relationship Of Nd1 Mtdna Gene Polymorphisms To Energy Metabolism And Risk Of Obesity Among Peruvian Native Americans, Karen K. Thomas, Dr. Mark J. Rowe

Journal of Undergraduate Research

Mitochondria are involved in the conversion of energy in the cell. Metabolic energy from the food we eat is converted in mitochondria to ATP by the electron transport system (ETS) and oxidative phosphorylation. The enzyme complexes involved in the ETS contain thirteen subunits which are encoded by mitochondrial DNA (mtDNA). Non-silent polymorphisms (sites in the DNA where the nucleotide differs between individuals) in mtDNA will affect these enzymes and may thereby alter metabolic efficiency and therefore metabolic rate. A lower metabolic rate is a risk factor for weight gain and a higher body mass index (BMI, a measure of obesity) …


Optimized Production Of Vanillin From Green Vanilla Pods By Enzyme-Assisted Extraction Combined With Pre-Freezing And Thawing, Yanjun Zhang, Limei Mo, Feng Chen, Minquan Lu, Wenjiang Dong, Qinghuang Wang, Fei Xu, Fenglin Gu Feb 2014

Optimized Production Of Vanillin From Green Vanilla Pods By Enzyme-Assisted Extraction Combined With Pre-Freezing And Thawing, Yanjun Zhang, Limei Mo, Feng Chen, Minquan Lu, Wenjiang Dong, Qinghuang Wang, Fei Xu, Fenglin Gu

Publications

Production of vanillin from natural green vanilla pods was carried out by enzyme-assisted extraction combined with pre-freezing and thawing. In the first step the green vanilla pods were pre-frozen and then thawed to destroy cellular compartmentation. In the second step pectinase from Aspergillus niger was used to hydrolyze the pectin between the glucovanillin substrate and β-glucosidase. Four main variables, including enzyme amount, reaction temperature, time and pH, which were of significance for the vanillin content were studied and a central composite design (CCD) based on the results of a single-factor tests was used. Response surface methodology based on CCD was …