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Screening Of Biocontrol Organisms For The Management Of Phytopathogenic Fungi And Foodborne Pathogens On Produce, Antoinette Boyee De Senna

Master's Theses

The multibillion dollar agricultural industry is an important part of the United States economy, and the management of factors that affect crop and human health is imperative to maintaining this economic sector. The fungi Botrytis cinerea, Fusarium pallidoroseum, and Fusarium moniliforme are the causative agents of several plant diseases and can cause significant crop loss both before and after harvest in commodities such as strawberries, lettuce, citrus, and grains. Fungicides are employed to control these phytopathogens, but the use of these chemicals has led to an increase in fungicide resistance and may negatively affect the environment and human …

Predictive Modeling Of Oxygen Transmission Through Micro-Perforations For Packaging Applications, Ayman Abdellatief, Bruce A. Welt, Jason Butler, Eric Mclamore, Arthur Teixeira, Sanjay Shukla

Journal of Applied Packaging Research

Methods for creating precise perforations in respiring produce packaging are being increasingly adopted. Knowledge of oxygen transfer through perforated packaging and oxygen distribution in packages is necessary for successful packaging design of fresh produce. An approach to modeling perforated packaging performance was developed using a cylindrical chamber with precision perforations using Fick’s second law. The model was simulated using two techniques including Finite Element Method (FEM) using commercially available software and Finite Volume Method (FVM) through programming. Perforations were approximated as a source term in the second method. Both simulation techniques showed trends similar to experimental data.

A Study Whether Humans Can Produce Free Fatty Acids For Tasting, Emily Bacher

Student Scholarship

A growing concern throughout the world, especially the United States, is the health of our population. According to the National Health and Nutrition Examination Survey (NHANES) performed in 2009-2010, approximately 35% of both men and women in the United States are obese (Flegal, 2012). In 2005, more than 300,000 deaths in the United States were attributed to obesity. However, the World Health Organization listed obesity as one of ten most preventable health risks (Wilborn, 2005).
Why, then, do we as a society struggle so much with our weight? Some, such as Swinburn, believe this trend of obesity can be attributed …

Combining Systems Methodologies To Reduce Allergen-Related Food Recalls, Jill Marie Sweney

Dissertations and Theses

The risk of poor food safety is a major focus for managers in the food manufacturing industry. Despite industry-led and regulatory efforts to improve the overall food safety of US packaged consumer foods, product recalls and market withdrawals are increasing. This is especially true for the most frequent cause for food recall: the undeclared allergen. With industry trends leaning toward adoption of third-party food safety management certifications, a popular food safety code from the Safe Quality Foods Institute is evaluated using Systems Analysis. Three changes to the food safety code are proposed to address three of the top causes for …

Identification Of Species In Commercially Sold Game Meats Using Dna Barcoding, Charles Quinto, Rebecca Tinoco, Rosalee S. Hellberg

Student Scholar Symposium Abstracts and Posters

Game meats represent a multibillion dollar industry in the United States with high economic incentives associated with species substitution and mislabeling. However, there is currently a lack of information regarding the prevalence of mislabeled game meat on the U.S commercial market. The purpose of this study was to conduct a market survey of whole-cut game products sold within the United States to identify incidences of mislabeling using DNA barcoding. Identified species were also examined for classification as a threatened or endangered species. Fifty-four whole-cut game meat samples were collected from online distributors in the United States and sequenced across the …

Effect Of Phytosanitary Irradiation On The Quality Of Chandler Pummelo (Citrus Maxima (Burm.) Merr.), Akanksha Jain, J.J. Ornelas-Paz, Karina Rodriguez, Anuradha Prakash

Student Scholar Symposium Abstracts and Posters

In this study, we evaluated the chemical and physiological effect of low-dose gamma irradiation on the post-harvest quality of Chandler Pummelos (Citrus maxima (Burm.) Merr.), an emerging crop of interest in the U.S. Chandler pummelos from a local grower in California were irradiated at target doses of 150 Gy and 1000 Gy. Irradiated and untreated pummelos were stored at 12 °C for 3 weeks and at 20 °C for the 4th week to reflect three weeks of sea shipment at ideal temperature for storage of pummelos and an additional week of retail under ambient conditions. Irradiation reduced hardness of the …

Assessing The Educational Program Needs Of Small And Limited Resource Meat Goat Producers, Francisca Quarcoo

College of Agriculture, Environment and Nutrition Sciences Graduate Theses and Dissertations

ABSTRACT

ASSESSING THE EDUCATIONAL PROGRAM NEEDS OF

SMALL AND LIMITED RESOURCE MEAT GOAT PRODUCERS

by

Francisca A. Quarcoo

The purpose of this study was to assess the preferred educational program needs of small meat goat producers. The specific objectives were to (1) determine if current educational programs by the Caprine Research and Education Unit and/or the Cooperative Extension Program, Tuskegee University, reflect the actual needs of meat goat producers, (2) measure adoption and impact of past educational efforts by the Caprine Research and Education Unit and/or the Cooperative Extension Program, (3) determine the desired presentation or delivery format for future …

The Effects Of Phytochemical Tannin-Containing Diets On Animal Performance And Internal Parasite Control In Meat Goats, Chassity Wright

College of Agriculture, Environment and Nutrition Sciences Graduate Theses and Dissertations

ABSTRACT

The Effects of Phytochemical Tannin-Containing Diets on Animal Performance and Internal Parasite Control in Meat Goats

By

Chassity Wright

Haemonchus contortus (H. contortus) resistance has been reported against almost all chemical anthelmintics available for its control. H. contortus has a remarkable ability to develop resistance and threatens the viability of the goat industry in many regions of the world. Consequently, there is an urgent need to understand the genetic mechanisms underlying anthelmintic resistance and to discover new alternative methods of chemical and non-chemical control. With chemical anthemintics failing, this has led to the evaluation of plants as a natural …

Insomniac Of The Soil: A Collection Of Poetry And Essays, Sarah E. Golibart

Senior Honors Projects, 2010-2019

“Insomniac of the Soil” is a homage to a landscape that has deeply informed Sarah Golibart's life and her artistic voice – the tidewater flatlands of Virginia’s Chesapeake Bay peninsula where her family lives and where Golibart has worked on farms since high school. Both her poems and essays are earthy, imagistic, and grounded – quite literally – in the soil as well as in a sensibility of ecological ethics and sustainability. “Insomniac of the Soil” is also a love song to the fervent and fallow cycles of the soil.

Establishing A Kitchen With Jkuat, Kenya [2015], Germaine Tuyisenge

Business and Social Enterprise

Internship Report 2015

From May to August,2015a group of four interns, from Western Universitywere in a placement at Jomo Kenyatta University of Agriculture and Technology(JKUAT), doing an internship with Western Heads East.The interns were working onFiti Projectfor the production of Probiotic Yoghurt. The team of interns was multidisciplinarywith the four students taking different programs at Western: Medical Sciences, finance, political sciences and health-geography. Thiswas an asset for the project,helpingto have a strong approach on how to make ita success, everyone bringing in her own expertise. Our overall goal for the projectwas toestablish a probiotic kitchen at JKUAT, and that kitchen …

Antimicrobial Susceptibility Of Listeria Monocytogenes To Bacteriophage Listex™ P100 In Alfalfa Sprouts (Medicago Sativa), Tushar Sawant

Food Science (MS) Theses

The seed germination process during sprout production provides suitable environmental conditions for the growth of pathogenic bacteria, such as Listeria monocytogenes. A potential way to control this bacterial growth is through the use of bacteriophages, which are naturally occurring viruses that specifically attack bacterial targets and have been shown to be effective antimicrobials in some foods. Therefore, the objective of this study was to evaluate the antimicrobial susceptibility of L. monocytogenes to bacteriophage on alfalfa sprouts during seed germination and subsequent refrigerated storage at 4 °C. Alfalfa sprout seeds were dip-inoculated with 5.5 x 105 CFU/ml L. monocytogenes serogroups 1 …

Chemical Separation And Analyses Of Bioactive Compounds In Chloroform Fraction Of Oplopanax Horridus (Devil's Club), Lauren Wiseman

All Theses

O. horridus (a member of the family Araliaceae) is a plant traditionally used by the tribe of Pacific Northwest America for medicinal as well as spiritual purposes. O. horridus is found in the temperate forests of northwestern North America as an understory shrub capable of growing in well-drained to poorly drained, shady sites. Traditional medical preparations of O. horridus include decoction or infusion, preparation as a tea, preparation as a poultice, or preparation as a tonic. O. horridus is used as a form of treatment for cancer, hypoglycemia, diabetes, tough pneumonia, and for colds. A practical and reliable extraction method …

Investigation Of Sugar/Polyols As Weakly Interacting Cosolvents And Their Influence On Hardening Of High-Protein Nutrition Bars, Sami Kadhim Hassan

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The Western Dairy Center (WDC) at Utah State University demonstrated opportunities to improve the quality of high-protein snack foods that becoming more prevalent in western diets. Previously, such high-protein nutrition bars became too hard during storage and they had a limited shelf life, resulting in disappointment by consumers or loss of product as older products needed to be discarded.

With sponsorship as a doctoral student by the Iraq Ministry of Higher Education and Scientific Research and in conjunction with WDC researchers, an investigation was conducted into the chemistry of high-protein nutrition bars and how the various components (protein, carbohydrate and …

Effect Of Germination And Parboiling On Milling, Physicochemical, And Textural Properties Of Medium- And Long-Grain Rough Rice, Ashley Han

Graduate Theses and Dissertations

Germinated brown rice is commonly consumed in Asia for its enhanced nutritional properties such as gamma-aminobutyric acid (GABA) and softer cooked texture. However germination decreases milling yield and alters some physicochemical properties, and most germinated rice studies evaluated medium-grain and aromatic rice cultivars. This study compared the effects of germination duration and parboiling on milling, physicochemical, and textural properties of a medium- (Jupiter) and a long-grain (Wells) rice cultivars. Rough rice was soaked in water at 25°C for 12 hr, and then incubated at 30-34°C for varying germination durations, or combined with parboiling at 120°C for 20 min prior to …

The Effects Of Electrostatic Spraying With Organic Acids In The Disintegration Of Biofilms Formed By E.Coli O157:H7 And Salmonella Typhimurium On Spinach And Cantaloupe, Ahmad Almasoud

Graduate Theses and Dissertations

Outbreaks from the consumption of fresh produce are a concern in the United States. The consumptions of fresh produce have increased recently which expose a large segment of society to such outbreaks. Spinach and cantaloupe are minimally heated or processed before consumption which makes them a possible source of foodborne illness. The objective of this research was to investigate the effect of organic acids alone and in combination to reduce attached Salmonella Typhimurium (S.T) and Escherichia coli O157:H7 (E.coli) on spinach and cantaloupe, and to disintegrate biofilm formed by these pathogens by electrostatically spraying with two organic acids. To quantify …

Effects Of Chemical And Enzymatic Modifications On The Starch-Inclusion Complex Formation, Emily Arijaje

Graduate Theses and Dissertations

Starch-inclusion complexes have been proposed as delivery tools for bioactive molecules; however complexation yield is generally low with low solubility, which may limit the bioavailability of the included molecule. It was proposed that chemical (acetylation) and/or enzymatic (isoamylase and beta-amylase) modifications of starches prior to complex formation with fatty acids of different structures, including stearic, oleic and linoleic acid, may help increase complexation yield and solubility of the resulting starch complexes. Potato starch had a significantly higher complexation yield than common corn and high amylose (~70%) corn starches after debranching combined with a beta-amylase treatment. Debranched waxy maize starch and …

Application Of Essential Oil Compounds And Bacteriophage To Control Staphylococcus Aureus, Anisha Ghosh

Graduate Theses and Dissertations

Staphylococcus aureus is one of the most important pathogens, causing various diseases in humans and animals. In addition, S. aureus is a common foodborne pathogen. As methicillin- resistant S. aureus (MRSA) becomes increasingly prevalent, controlling this pathogen in animals and humans with standard antibiotic treatment has become challenging. Combinations of different antimicrobial agents represent one of the most promising approaches for combating multidrug - resistant bacteria both for treatment of clinical disease as well as in food. Two such antimicrobials with potential application in the food industry include essential oils (EO) and host-specific bacteriophage (phage). The objectives of this study …

Corn Characterization And Development Of A Convenient Laboratory Scale Alkaline Cooking Process, Shreya N.O. Sahasrabudhe

Department of Food Science and Technology: Dissertations, Theses, and Student Research

Nixtamalized (alkaline cooked) corn (Zea mays L.) products are increasing in popularity due to their affordable cost, ease of production, and the diversity of products that can be made using similar unit operations. The nixtamal produced after alkaline cooking depends on the processing parameters used during cooking and steeping, as well as the physicochemical properties of the corn hybrids used. Processors incur high costs in narrowing down hybrids suitable for a given process, or they must be able to adjust cooking conditions to obtain the desired end-product characteristics. Improper processing generates large quantities of waste. Researchers have developed small …

Viability Of Lactobacillus Acidophilus Dds 1-10 Encapsulated With An Alginate-Starch Matrix, Liya Mo

Department of Food Science and Technology: Dissertations, Theses, and Student Research

L. acidophilus DDS1-10 was encapsulated using an alginate, potato starch and type 4 resistant starch (RS4) matrix. The wall material was optimized by varying levels of alginate (1.6 to 4.4%), while maintaining potato starch and RS4 ratio fixed at 2, and the total solid content at 5%. Particles were prepared using an emulsion and an extrusion method. Particles obtained from the emulsion method were smaller with a non-uniform distribution in size while those obtained by the extrusion method were bigger and uniform in size. The emulsion method did not seem to offer protection to the probiotic cells against pH, bile …

Transport Of Bovine Milk Exosomes By Human Colon Carcinoma Caco-2 Cells And Rat Small Intestinal Iec-6 Cells, Tovah Wolf

Department of Nutrition and Health Sciences: Dissertations, Theses, and Student Research

We have reported that microRNAs (miRNAs, miRs) in bovine milk regulate human genes. In milk, many miRNAs are encapsulated in exosomes, thereby conferring protection against degradation and a pathway for intestinal transport of miRNAs. We hypothesized that the uptake of bovine exosomes in human intestinal colon carcinoma Caco-2 cells and rat primary small intestinal IEC-6 cells is mediated by endocytosis. Transport studies were carried out using fluorophore-labeled exosomes purified from bovine milk. The transport of bovine exosomes exhibited saturation kinetics at 37°C (K_m= 55.5±48.6 µg/200 µL, V_max= 0.083±0.057 ng exosomal protein x 81,750 cells⁻¹ x …

Understanding Perceptions Of Genetically Modified Food Among Natural Food Shoppers In Southern Nevada, Christian Murua-Aceves

UNLV Theses, Dissertations, Professional Papers, and Capstones

Today, 85% of corn, 93% of soybeans, and 88% of cotton in the U.S. are genetically modified (GM). Using laboratory methods, genes from one species, such as a plant, can be transferred to an unrelated species, such as an animal. Genetically modified crops may lead to higher yields, have improved food quality, texture, and nutritional value, and have an increased shelf life.

Despite these promising benefits, the potential health risks relating to the consumption of GM food remain inadequately assessed. Genetically modified food is not subjected to rigorous safety testing, such as epidemiologic studies, to identify potential health risks. Two …

Comparison Of Item Response Theory Models For Data Fit Of Quality Control Measurements For Maillard Reaction Browning In Foods, Polly Barrett

Dissertations

In foods, browning is a complex set of reactions known as the Maillard Reaction (MR). As food is cooked, MR yields flavor, color and changes to the chemical properties of the initial food item. It is possible to undercook or overcook the food item, resulting in a less browned or burnt product. Item response theory (IRT) is often used to place subjects and instruments on the same continuum of the measured latent trait. IRT has been widely used in the fields of educational measurement and psychology, but few studies combine IRT and food science. This research tested IRT modeling for …

Cla-Rich Soy Oil Shortening, Chocolate Paste, And Chocolate Bar Production And Characterization, Elizabeth Sarah Mayfield

Food Science Undergraduate Honors Theses

Conjugated linoleic acid-rich soy oil (CLARSO) has been shown to have numerous health benefits, including anti-obesity and anti-carcinogenic properties. This oil was previously used to produce CLA-rich margarine that provided the recommended daily value of CLA and showed physical characteristics similar to commercially available margarine. The objectives of this study were to produce CLA-rich shortenings and analyze their physical properties relative to commercially available shortenings and soy oil control shortenings and to produce CLA-rich chocolate bars and pastes by replacing a portion of the fat with CLARSO and compare the physical properties of these pastes/bars to controls made with either …

Characterization Of A Sample Population Of Dental Hygiene Patients With And Without Periodontal Disease As Determined Through Fatty Acid And Fruit And Vegetable Intake, Caitlin R. Jackson

Undergraduate Honors Theses

This study was designed to compare the fatty acid and fruit and vegetable intake of people with and without periodontal disease. Periodontal disease affects approximately 15 percent of the developed world population. Participants filled out a food frequency questionnaire (FFQ) for both fruit and vegetable and fat intake. A sample of whole blood (EDTA) was collected to determine the fatty acid profile of participants’ red blood cell (RBC) membrane. This analytical procedure determines the average fatty acid intake of the participant over the previous 90 to 120 days. Participants were classified as case (n=10, patients diagnosed with periodontal disease) or …

Effects Of Beef Finishing Diets And Muscle Type On Meat Quality, Fatty Acids And Volatile Compounds, Arkopriya Chail

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Consumer evaluation, proximate data, Warner-Bratzler shear force (WBSF), fatty acid (FA) composition and volatile compounds were analyzed from the ribeye steaks (LT) finished on conventional feedlot (FL) and forages, including a perennial legume, birdsfoot trefoil (BFT; Lotus corniculatus), and a grass, meadow brome (Bromus riparius Rehmann, Grass). Representative retail forage (USDA Certified Organic Grass-fed, COGF) and conventional beef (USDA Top Choice, TC) were investigated (n = 6) for LT. Additionally, the effects of diet on round (GM) and chuck (TB) muscles FL, BFT and Grass were explored. Forage-finished beef was less liked in most of the affected …

Analysis Of Quality Of Two Different Varieties Of Peaches With Respect To Organic And Conventional Cultivation Techniques, Shruti D. Sawant

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The worldwide demand for organic produce has been on the rise in recent years. This is a result of consumer concerns about the environment and chemicals used in food production. In addition, consumers have demonstrated that they are willing to pay premium prices for organic produce based on the general assumption that organic produce is more nutritious, environmentally friendly, and better-tasting. There have been several studies that have reported significant differences with organic and conventionally grown produce. Organic fruits and vegetables have been shown to have higher dry matter, antioxidants. In addition, it has also been shown to be smaller …

Pregenomic And Genomic Effects Of 24,25-Dihydroxyvitamin D3, Yang Zhang

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

In the United States, the major dietary source of protein is poultry meat, particularly chicken. Phosphorus absorption in chickens is a critical problem in poultry production. It therefore is important to understand the cellular and molecular regulation of phosphorous absorption in intestine to increase efficiency of the process. This would provide the benefits of reducing feed costs and reducing phosphorous excretion, thereby contributing to the sustainability of agriculture in the United States. Therefore, the focus of this thesis is determining the effect of vitamin D metabolites involved in phosphate homeostasis using the chicken as a model.

Self-Learning, Dvd-Based Education Versus Traditional Education Approaches To Improve The Safety Of Warfarin Use Among Patients With Atrial Fibrillation, Jessica Oliver Hatch

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Atrial fibrillation (AF) is a common cardiac arrhythmia that requires extensive medical and pharmaceutical management. The coagulation antagonist warfarin is commonly prescribed to reduce AF-associated stroke. Although warfarin effectively mediates thromboembolitic risk, its management is complex as many factors influence its therapeutic range including: genetics, diet, medication, and herbal and dietary supplement (HDS) interactions. Lack of patient knowledge regarding these factors contributes to poor patient outcomes. With the emerging epidemic of AF, readily available educational tools are necessary to improve patient outcomes while reducing clinician burden.

The purpose of this study was to develop both a self-learning, DVD-based and one-on-one …

Optimizing Care Through Rd Checks, Suzanne Ickes Rd, Ldn, Melissa Faura Rd, Ldn, Ann Flickinger Ms, Rd, Ldn, Kristin Titus Rd, Ldn

Clinical Nutrition Service

No abstract provided.

Characterization And Investigation Of Fungi Inhabiting The Gastrointestinal Tract Of Healthy And Diseased Humans, Mallory J. Suhr

Department of Food Science and Technology: Dissertations, Theses, and Student Research

Gastrointestinal microbiome studies have failed to include fungi in total community analyses. As a result, their diversity and function in the gut is poorly understood. Recent work has begun to uncover the role intestinal fungi play in diet, immune system development, interactions with other microorganisms in the gut, and pathogenesis of diseases. Advances in sequencing technologies allow for the ability to profile the fungal gut microbiome (“mycobiome”) in healthy and diseased states. This thesis explores the mycobiome in 1) healthy humans with a vegetarian diet and 2) pediatric small bowel transplant recipients that develop fungal bloodstream infections.

The gut mycobiome …