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Use Of Rhodamine B As A Biomarker In A Simulated Oral Vaccine Deployment Against Bovine Tuberculosis In White-Tailed Deer, David Dressel, Kurt Vercauteren, Michael Lavelle, Nathan P. Snow, Henry Campa Iii

United States Department of Agriculture Wildlife Services: Staff Publications

Introduction Free-ranging white-tailed deer (Odocoileus virginianus) in northeastern lower Michigan, United States are a self-sustaining reservoir for bovine tuberculosis (bTB). Farm mitigation practices, baiting bans, and antlerless deer harvests have been ineffective in eliminating bTB in white-tailed deer and risks to cattle. The apparent prevalence has remained relatively constant in deer, prompting interest among wildlife researchers, managers, and veterinarians for an effective means of vaccinating deer against bTB. The commonly used human vaccine for bTB, Bacillus Calmette Guerin (BCG), is the primary candidate with oral delivery being the logical means for vaccinating deer.

Materials and methods We developed …

Effects Of Pregnancy Prevention On Brucella Abortus Shedding In American Bison (Bison Bison), Pauline Nol, Rebecca Frey, Morgan Wehtje, Jack C. Rhyan, Patrick Ryan Clarke, Matthew Mccollum, Christine Quance, Douglas C. Eckery, Suelee Robbe-Austerman

United States Department of Agriculture Wildlife Services: Staff Publications

Products of parturition are the predominant source of Brucella abortus for transmission in bison (Bison bison). Our objective was to assess whether preventing pregnancy in Brucella-seropositive bison reduced B. abortus shedding. Brucella-seropositive and -seronegative bison from Yellowstone National Park, Wyoming, USA were used in a replicated experiment. Each of two replicates (rep1, rep2) included a group of seropositive females treated with a single dose of gonadotropin-releasing hormone-based immunocontraceptive (Treatment rep1, n = 15; Treatment rep2, n = 20) and an untreated group (Control rep1, n = 14; Control rep2, …

Population Growth Rates Of Pronghorn: Influence Of Temporally And Spatially Explicit Conditions, Density Dependence, And Scale, Eric M. Gese, Cole A. Bleke, Shane B. Roberts, Paul Atwood, Patricia A. Terletzky

United States Department of Agriculture Wildlife Services: Staff Publications

Density-independent and density-dependent population regulation has long been a subject of investigation. We examined density-dependent and density-independent factors on growth rates of pronghorn (Antilocapra americana (Ord, 1815)) using a retrospective analysis of population survey data. Across Idaho, we found that as the proportion of the subpopulation harvested the previous year increased, growth rates increased. Similarly, as fawn recruitment increased, growth rates increased. We also found when the growth rate in the previous year increased, the growth rate the subsequent year decreased. When subpopulations were examined independently, we found that in a low-elevation desert subpopulation, growth rates were influenced by …

Invasive Wild Pig (Sus Scrofa) Diets On Barrier Islands In The Southeastern United States, Vienna R. Canright, Antoinette J. Piaggio, James C. Beasley

United States Department of Agriculture Wildlife Services: Staff Publications

Background

Biological invasions are a leading cause of reductions in global biodiversity. Islands are particularly sensitive to invasions, which often result in cascading impacts throughout island communities. Wild pigs (Sus scrofa) are globally invasive and pose threats to numerous taxa and ecosystems, particularly for islands where they have contributed to declines of many endemic species. However, the impacts of wild pig diet on the flora and fauna remain understudied in many island systems.

Results

We used DNA metabarcoding of wild pig fecal samples to quantify the seasonal diet composition of wild pigs on three barrier islands in the …

Taphonomic Signatures Of Early Scavenging By Black And Turkey Vultures, Marian L. Wahl, Grant N. Burcham, Amanda M. Herbert, Lee A. Humberg, Patrick A. Zollner, Landon R. Jones, Brandon M. Quinby, Bryan M. Kluever

United States Department of Agriculture Wildlife Services: Staff Publications

Scavenging is critical for nutrient cycling and maintenance of healthy ecosystems. While there is substantial research into the identification of taphonomic signatures from facultative mammalian scavengers, early stage scavenging signatures by vultures remain unknown. Further, some vulture species are opportunistic predators, highlighting the need to define signatures observed in the course of normal scavenging behavior. We placed stillborn neonatal calves in an unoccupied pasture and used motion-trigger camera traps to quantify scavenging effort, then conducted necropsies to evaluate the effect of black vulture (Coragyps atratus) and turkey vulture (Cathartes aura) scavenging effort on carcass consumption. We …

Temporal And Spatial Influences On Fawn Summer Survival In Pronghorn Populations: Management Implications From Noninvasive Monitoring, Cole A. Bleke, Eric M. Gese, Juan J. Villalba, Shane B. Roberts, Susannah S. French

United States Department of Agriculture Wildlife Services: Staff Publications

Monitoring vital rates allowsmanagers to estimate trends in growth rates of ungulate populations. However, connecting the influence of nutrition on ungulate demography is challenging. Noninvasive sampling offers a low-cost, low-effort alternative for measuring nutritional indices, allowing for an increased understanding of the mechanistic relationships between environmental factors, nutrition, and specific population vital rates. We examined the temporal influence of intrinsic and extrinsic factors on pronghorn (Antilocapra americana) fawn recruitment. We collected fresh fecal samples from adult female pronghorn in five subpopulations spanning three sampling periods associated with critical maternal life-history stages (late gestation, early lactation, breeding season) for …

Surveillance Of Highly Pathogenic Avian Influenza Virus In Wild Canids From Pennsylvania, Usa, Kevin D. Niedringhaus, Taylor C. Chan, Ashley Mcdowell, Lauren Mxwell, Madison Stevens, Lane Potts, Erica Miller, Eman Anis, Kyle Van Why, Thomas Keller, David Stallknecht, Rebecca L. Poulson, Kaitlyn Bahrs, Justin D. Brown

United States Department of Agriculture Wildlife Services: Staff Publications

The avian influenza virus is a global pathogen with significant health and economic implications. While primarily a pathogen of wild and domestic birds, recent outbreaks of the H5N1 highly pathogenic avian influenza virus (HPAIV) clade 2.3.4.4b have caused mortality in a wide variety of mammals, including members of the Canidae family, on multiple continents. Despite sporadic mortality events globally, the epidemiology and pathobiology of H5N1 HPAIV in wild canids remains poorly defined. During 2022–2024, 41 wild canid carcasses (diagnostic cases), including 23 red foxes and 18 gray foxes, were tested for the influenza A virus (IAV) via PCR, with five …

Exploring The Connection: Field Ecology Research And Student Wellbeing, Elizabeth Small

Graduate Research Theses & Dissertations

Field work is essential in ecological research, offering the opportunity to gather firsthand data, observe natural processes, and deepen our understanding of ecosystems. In my study on small mammal trophic niche dynamics, I investigate how small mammals—critical players in grassland food webs—adapt their diets in response to environmental disturbances. By examining the drivers of variation in trophic niche among three common small mammal species, I aim to uncover how these animals coexist despite similar dietary requirements. Understanding these dynamics is critical for informing global conservation efforts, especially as we work to reverse the biodiversity crisis and restore damaged ecosystems.However, the …

Metal Contamination In Household Dust And Their Health Risk Assessment: A Study In Two Malaysian Cities, Xin Yi Lim, Ting Fang Lye, Joo Hui Tay

Applied Environmental Research

Investigating the composition of household dust can provide crucial insights into potential environmental and health implications. This study aimed to determine the concentration of selected metals in 30 household floor dust samples collected from two cities in Peninsular Malaysia, namely Melaka and Butterworth. The samples were collected using nylon socks attached to a vacuum cleaner nozzle during January-February 2021. All samples were sieved through a 200-µm sieve, acid-digested with aqua regia, and analyzed using inductively coupled plasma mass spectrometry (ICP-MS). Mean metal concentrations decreased in the order of Fe>Al>Mg>Zn>Mn>Ba>Cu>Cr>Pb. Cd was not …

Exposure To Metal Accumulation In Chicken Giblets And Human Health Risk In Lokoja, Nigeria, Jude Ehwevwerhere Emurotu, Lami Habib, Ephraim Musa Dallatu, Adamu Shuaibu Onakpa, Aloysius Akaangee Pam

Applied Environmental Research

When consumed, the toxic heavy metals in chicken giblets pose a grave risk to humans. An inductively coupled plasma optical emission spectrometer (ICP-OES) was used to measure the concentrations of metals (Al, Cd, Co, Cr, Ni, Mn, and Pb) in the liver, gizzard, and kidney of chickens from the Lokoja poultry market. Except for lead, which slightly exceeded the FAO/WHO limit of 0.1 mg kg-1, the metal concentrations found were all within the threshold limit. The estimated daily intake (EDI), hazard quotient (HQ), hazard index (HI), and cancer risk (CR) were used to assess the risks that humans would face …

Outlooks And Challenges For Urban Green Space Development: A Review Case Study In Thailand, Kanawat Paoin, Chanathip Pharino

Applied Environmental Research

Even though prior studies have provided ample proof of the benefits of urban green space, Thailand's development and exploitation of urban green space remain insufficiently efficient. To assess the prospects for urban green space development in Thailand, our review study highlighted gaps and challenges related to these areas. This review study discussed the rationale for urban green space, its definitions, as well as its benefits and co-benefits (i.e., economic, social, health, and environmental). The review research additionally discussed Thailand's current urban green space issues, associated challenges (such as difficulties valuing and using urban green space, budgetary limitations, low priority for …

Path Analysis Of The Relationship Between Diarrhea, Climate And Environmental Variables In Province Of West Nusa Tenggara-Indonesia, Khadijah Azhar, Ika Dharmayanti, Dwi Hapsari Tjandrarini, Bambang Wispriyono

Applied Environmental Research

Diarrhea is a major cause of morbidity and mortality, particularly in West Nusa Tenggara Province, Indonesia, due to certain factors. Therefore, this study aims to examine the influence of climate, economic status, demographic, water, sanitation, and hygiene factors on diarrhea. The study procedures were carried out using an ecological design that focused on the characteristics of the sample population. The unit of analysis was districts/cities, where data on diarrhea cases from 2017–2020 were used, along with other variables in the same period. The dependent variable was the number of diarrhea cases per month, while the independent variables included average temperature, …

Addressing The Consumer Food Waste Crisis: A Decade Of Psychological Interventions, Pei ๋Jun Woo, Yohanna Veshanya, Lai Ti Gew

Applied Environmental Research

Food waste has become an increasing global crisis, especially in the last few years. Researchers and policymakers have attempted to work towards meeting the Sustainable Development Goals (SDGs) proposed by the United Nations. While much research has been conducted, we are still far from achieving the SDG by 2030. Many interventions have been studied, but each outcome varies. Thus, this review summarizes 30 psychological-based interventions between 2010 to 2022, retrieved via the Scopus database. It is found that each method of intervention works differently based on each type of food waste. Institutional food waste appears to be the most common …

Theoretical Approaches To Food Waste Behaviours: An Overview, Dane Chia, Chin Choo Yap, Mohamed Kheireddine Aroua, Lai Ti Gew

Applied Environmental Research

Food waste around the world has emerged as a pressing issue that calls for attention and intervention. Analysing the food waste problem without understanding wasteful behaviour antecedents may oversimplify this multi-faceted issue, which will then compromise the development of interventions. This paper provides an overview of the theoretical approaches to food waste behaviour antecedents, followed by evidence-based interventions, using the Theory of Planned Behaviour (TPB), the Norm Activation Model (NAM), and the Social Practice Theory (SPT). These theories encompass the psychological, behavioural, societal, material, and structural factors that influence food waste practices. Our aim is to provide a holistic understanding …

Unraveling The Effects Of Arbuscular Mycorrhizal Fungi On Plant Growth, Nutrient Content, And Heavy Metal Accumulation In The Contaminated Soil: A Meta-Analysis, Bela Putra, Anuraga Jayanegara, Irwan Susanto

Applied Environmental Research

This study evaluated the effects of arbuscular mycorrhizal fungi (AMF) on plant growth, nutrient uptake, and heavy metal accumulation on polluted land using a meta-analysis approach. Data from 33 relevant studies were selected based on inclusion criteria, specifically articles in English, observational research, and investigating the role of AMF in plant growth and productivity on polluted land. The results showed that plants inoculated with AMF experienced significant accumulation of heavy metals in roots, such as Pb (p<0.01), Ni (p<0.01), Cr (p<0.01), Mn (p<0.05), Fe (p<0.05), and As (p<0.05). The AMF significantly reduced the accumulation of heavy metals such as Cr, Ni, Fe, and Cu on the upper part of fodder forage (p<0.01). Forage growth was also enhanced due to AMF. The AMF greatly increased the fresh weight, length, and phosphorus (P) content of fodder forage roots (p<0.01). It also increased the plant's biomass, fresh weight, dry weight, height, nitrogen (N), phosphorus (P), and potassium (K) contents (p<0.01). In conclusion, AMF is important in increasing plant growth, nutrient uptake and reducing heavy metal accumulation in forage on polluted land.

The Influence Of Chemical Fertilizers On The Effectiveness Of Biochar In Mitigating Cadmium Mobility In Soil, Chompoonut Chaiyaraksa, Navapat Sangworn

Applied Environmental Research

The focus of this study was to explore how various fertilizers influence the movement of cadmium in soil treated with biochar. The research utilized a strong acid sandy loam soil from Chanthaburi Province, naturally rich in organic matter with moderate cation exchange capacity and low nitrogen, potassium, phosphorus, sulfate, salinity and chloride levels. The soil was purposely contaminated with 50 mg kg-1 of cadmium and treated with biochar derived from water hyacinth through pyrolysis at 450°C for an hour. This biochar displayed moderate alkalinity, high organic matter, phosphorus, potassium, and cation exchange capacity, but low nitrogen content. Analytical techniques like …

Simulated Microgravity, Indole-3-Acetyl Acid, And Cow Dung Influence On Zea Mays Plant Growth In Contaminated Soil, A. Ovenseri, A.O. Ogunkeyede, A.A. Adebayo, P. Tawari-Fufeyin, A.O. Orukpe, C. Udeochu Uche

Applied Environmental Research

This study investigated the effects of simulated microgravity, indole-3-acetic acid (IAA), and cow dung on Zea mays L. plants growing in soil contaminated with crude oil. Crude oil contamination affects plants negatively in morphology and physiology. For simulated microgravity exposure, IAA introduced and non-IAA introduced seeds of Zea mays L. were grown in a clinostat for 4days before taken to the nursery for planting. IAA was introduced to the seed by soaking in IAA solution for 90 minutes after which it was introduced to the microgravity stimulator (clinostat). After 14 days in the nursery, the seedlings were taken to the …

Assessing The Relationship And Effect Of Air Pollution [Pm 2.5] On Child Respiratory Illness And Child Mortality In The Philippines, Roel F. Ceballos, Rachell C. Gallano, Lenard D. Visaya

Applied Environmental Research

Air pollution is the presence of harmful substances in the air, which can seriously impact human health and the environment. Among the air pollutants, fine particulate matter (PM) 2.5, being a microscopic particle, poses serious health risks since it can enter the blood circulation and go deep into the lungs. PM 2.5 has been linked to several health problems, such as early mortality, problems with children's development, and unfavorable birth outcomes. In this regard, the study aims to investigate the relationship and effect of PM 2.5 on child respi-ratory illness and child mortality in the Philippines. Data on child respiratory …

A Review On Chemometrics And Climate Change: Insights From Malaysia Case Study, Ismafatin Nabilah Ismail, Mohd Khairul Amri Kamarudin, Wan Nor Azilawanie Tun Ismail, Noorjima Abd Wahab, Ahmad Shakir Mohd Saudi, Mohd Ekhwan Toriman, Firdaus Mohamad Hamzah, Septi Ariadi

Applied Environmental Research

Climate change consider as main issues and concerns in Malaysia over the decade. Chemometrics techniques have been used in many climate change issues to address the limitations of climate change monitoring studies. In climate change areas, chemometrics is a valuable tool for assessing the correlation between different climate change factors, especially in vast and intricate databases. The purpose of the review was to evaluate and summarise the available data and restrictions regarding the use of chemometrics techniques in Malaysian environmental studies. The study conducted a thorough analysis of pertinent scientific papers published between 2019 to 2023 that addressed the main …

Optimizing Tenebrio Molitor Feeding Strategy To Induce Physical Degradation Of Tires: A Novel Approach For Sustainable Waste Management, Abdussalam Topandi, Khadijah Sayyidatun Nisa, Fatimah Azizah Riyadi, Ibnu Maulana Hidayatullah, Pranata -, Firah Gustianda Ayu, Reynaldi Ivan Prasetya, Frederick Soetandar, Dimas Rafi Narawangsa, Eka Rahayu

Applied Environmental Research

Due to the increased usage of tires, the accumulation of worn tires has become a significant environmental concern and necessitates environmentally friendly waste management strategies. Conventional methods, such as tire incineration or improper disposal, contribute to environmental degradation. It is reported that organisms, such as Tenebrio molitor larvae, can degrade a wide range of polymers. This study explores the promising potential of Tenebrio molitor larvae in addressing this concern. Through a series of experiments, Tenebrio molitor larvae were fed with cut-worn tires to facilitate degradation. The larvae exhibited remarkable capabilities, surviving and thriving on tires as a sole carbon source …

Barn Owls Exert Top-Down Effects On The Abundance And Behavior Of Rodent Pests, Katherine C. Larson

Cal Poly Humboldt theses and projects

Predators can exert top-down effects on their prey by direct consumption, by imposing risk of predation, or a combination of both. However, empirical evidence for these effects is mixed and inconsistent. Barn owls in agriculture present a system with attributes that should, according to theory, lead to strong top-down effects, but research is limited and the distinction between effects on prey abundance and behavior remains unresolved. Understanding the top-down effect of predators on pests is a cornerstone of integrated pest management and important where barn owls may contribute to the control of rodents on farms. Vegetation can mediate the top-down …

The Attitudes And Practices Of United Arab Emirates Consumers Towards Food Waste: A Nationwide Cross-Sectional Study, Lynne Kennedy, Samir Safi, Tareq M. Osaili, Ala Al Rajabi, Ayesha Alblooshi, Dima Al Jawarneh, Ahmed Al Kaabi, Fakhra Al Rubaei, Maitha Albreiki, Maryam Alfadli, Aseilah Alhefeiti, Moez Al Islam Ezzat Faris, Kholoud Allaham, Sameeha Junaidi, Moien A.B. Khan

All Works

Background: Reducing global food waste is an international environmental, health, and sus-tainability priority. Although significant reductions have been achieved across the food chain, progress by UAE households and consumers remain inadequate. This study seeks to understand the association between consumer attitudes, knowledge, and awareness relating to food waste practice of residents living in the UAE. to help inform policy and action for addressing this national priority. Methods: A cross-sectional study was conducted using a validated semi-structured online questionnaire through stratified sampling (n =1052). The Spearman correlation coefficient was performed to determine the correlations. Two independent regression analysis were used to …

Moab Local Food Guide 2024, Roslynn Mccann, Shiree Duncan

All Current Publications

Thank you for picking up this Moab Utah Local Food Guide. Those growing and producing food within a 100 mile radius from Moab have been included in this guide to help YOU make locally sourced purchasing decisions!

Estimating Home Range Size And Density Of White-Tailed Deer (Odocoileus Virginianus) In West Virginia, Sarah M. Pesi

Graduate Theses, Dissertations, and Problem Reports (ETD)

Big game hunting is an important source of revenue and recreation in the United States. Of big game species, white-tailed deer (Odocileus virginianus) are the most heavily pursued. White-tailed deer are a widespread generalist species, capable of existing at high densities. Hunting is often used as the primary method of managing deer populations, particularly in the eastern United States, where there is a lack of large predators. When populations reach high densities, there can be negative effects on the environment and conflicts with humans. Managers must consider both creating adequate hunting opportunities and reducing negative impacts when managing …

Widespread Crab Burrows Enhance Greenhouse Gas Emissions From Coastal Blue Carbon Ecosystems, Kai Xiao, Yuchen Wu, Feng Pan, Yingrong Huang, Hebo Peng, Meiqing Lu, Yan Zhang, Hailong Li, Yan Zheng, Chunmiao Zheng, Yan Liu, Nengwan Chen, Leilei Xiao, Guangxuan Han, Yasong Li, Pei Xin, Ruili Li, Bochao Xu, Faming Wang, Joseph J. Tamborski, Alicia M. Wilson, Daniel M. Alongi, Isaac R. Santos

OES Faculty Publications

Fiddler crabs, as coastal ecosystem engineers, play a crucial role in enhancing biodiversity and accelerating the flow of material and energy. Here we show how widespread crab burrows modify the carbon sequestration capacity of different habitats across a large climatic gradient. The process of crab burrowing results in the reallocation of sediment organic carbon and humus. Crab burrows can increase more greenhouse gases emissions compared to the sediment matrix (CO2: by 17-30%; CH4: by 49-141%). Straightforward calculations indicate that these increased emissions could offset 35-134% of sediment carbon burial in these two ecosystems. This research highlights the complex interactions between …

Digital Technologies For Environmental Sustainability, Raimundo Macêdo, Marcelo Pias, Liwan Liyanage, David M. Cook, Yuko Murayama, Otthein Herzog, Gülgün Kayakutlu, David Kreps

Research outputs 2022 to 2026

Recognizing the immense challenges posed by climate change and aligning with the UN Sustainable Development Goals (SDGs), particularly SDG 13, which focuses on climate action, the International Federation for Information Processing (IFIP) established the Digital Technology and Climate Change Task Force in December 2022. The Task Force aims to identify opportunities to leverage digital technologies effectively in combating climate change and to provide recommendations for professionals and relevant institutions. Since its inception, the Task Force has met regularly. After conducting a scoping exercise and defining a taxonomy, a comprehensive questionnaire was distributed twice among IFIP stakeholders, namely, technical committees and …

Global Meta-Analysis Of Demersal Fishing Impacts On Organic Carbon And Associated Biogeochemistry, Justin Tiano, Emil De Borger, Sarah Paradis, Clare Bradshaw, Claudia Morys, Antonio Pusceddu, Claudia Ennas, Karline Soetaert, Pere Puig, Pere Masqué, Marija Sciberras

Research outputs 2022 to 2026

The potential threat of fisheries on seabed carbon is a topic of growing concern, yet existing literature presents inconsistencies leaving experts divided on the topic. We conducted a global meta-analysis to synthesize the current knowledge and quantify how demersal fishing impacts various biogeochemical properties. Direct impact studies revealed overall reductions in chlorophyll-a (Chl-a, 17%), phaeopigments (24%), and proteins (32%). Effects on these reactive compounds were more pronounced on surface sediment (0–2 cm), where the impact on total organic carbon (TOC) also became significant, demonstrating the effect of gear penetration, and highlighting that sampling strategies combining sediment layers can mask observed …

Reducing Food Scarcity: The Benefits Of Urban Farming, S.A. Claudell, Emilio Mejia

Journal of Nonprofit Innovation

Urban farming can enhance the lives of communities and help reduce food scarcity. This paper presents a conceptual prototype of an efficient urban farming community that can be scaled for a single apartment building or an entire community across all global geoeconomics regions, including densely populated cities and rural, developing towns and communities. When deployed in coordination with smart crop choices, local farm support, and efficient transportation then the result isn’t just sustainability, but also increasing fresh produce accessibility, optimizing nutritional value, eliminating the use of ‘forever chemicals’, reducing transportation costs, and fostering global environmental benefits.

Imagine Doris, who is …

Tiny Drifters Amidst Global Change: Examining Environmental Drivers, Trophic Impacts, And Management Strategies Of Estuarine Plankton Communities In The Anthropocene, Taylor Nicole Dodrill

Dissertations and Theses

Plankton productivity supports estuarine food webs, and has been tied to the success of fisheries, macroinvertebrates, and cultured shellfish yields. Climate change and alterations to nutrient loads are thought to be influencing plankton assemblages, with toxin-producing harmful algal blooms (HABs) on the rise and nutritional quality of plankton declining globally. These shifts in plankton communities may contribute to low biomass yields and toxin-based closures of important fisheries. The objectives of this dissertation are to identify environmental drivers, trophic impacts, and management strategies to understand and respond to changing estuarine plankton communities. To address these objectives, I used a combination of …

Effects Of Arbuscular Mycorrhizal Fungi Inoculation And Cover Crop On Soil Carbon Dynamics And Microbial Communities In A Mediterranean Lemon Orchard., Anna Rodriguez-Paiatsyka

Master of Science in Environmental Sciences and Management Projects

Plant-associated fungi such as arbuscular mycorrhizal fungi (AMF) have the potential to sequester carbon (C), improve soil aggregation, and promote plant health. Due to multiple benefits of AMF to plant and soil health, AMF has gained much attention leading to a rapidly expanding market in mycorrhizal bio-stimulants intended to improve crop yield, root development, and soil health in horticultural crops, including citrus. However, there is limited information on how to inoculate a mature citrus orchard, and how inoculation of a mature orchard with AMF affects C sequestration. In this study, we planted a cover crop inoculated with AMF in a …