Digital Commons Network^™

Under-Oil Autonomously Regulated Oxygen Microenvironments: A Goldilocks Principle-Based Approach For Microscale Cell Culture, Chao Li, Mouhita Humayun, Glenn M. Walker, Keon Young Park, Bryce Connors, Jun Feng, Molly C. Pellitteri Hahn, Cameron O. Scarlett, Jiayi Li, Yanbo Feng, Ryan L. Clark, Hunter Hefti, Jonathan Schrope, Ophelia S. Venturelli, David J. Beebe

Faculty and Student Publications

Oxygen levels in vivo are autonomously regulated by a supply–demand balance, which can be altered in disease states. However, the oxygen levels of in vitro cell culture systems, particularly microscale cell culture, are typically dominated by either supply or demand. Further, the oxygen microenvironment in these systems is rarely monitored or reported. Here, a method to establish and dynamically monitor autonomously regulated oxygen microenvironments (AROM) using an oil overlay in an open microscale cell culture system is presented. Using this method, the oxygen microenvironment is dynamically regulated via the supply–demand balance of the system. Numerical simulation and experimental validation of …

Evaluation Of The Carotid Artery Using Wavelet-Based Analysis Of The Pulse Wave Signal, Sameh El-Sharo, Amani Al-Ghraibah, Jamal Al-Nabulsi, Mustafa Muhammad Matalgah

Faculty and Student Publications

The use of pulse wave analysis may assist cardiologists in diagnosing patients with vascular diseases. However, it is not common in clinical practice to interpret and analyze pulse wave data and utilize them to detect the abnormalities of the signal. This paper presents a novel approach to the clinical application of pulse waveform analysis using the wavelet technique by decomposing the normal and pathology signal into many levels. The discrete wavelet transform (DWT) decomposes the carotid arterial pulse wave (CAPW) signal, and the continuous wavelet transform (CWT) creates images of the decomposed signal. The wavelet analysis technique in this work …

Analysis Of An Existing Method In Refinement Of Protein Structure Predictions Using Cryo-Em Images, Maytha Alshammari, Jing He, Willy Wriggers, Jiangwen Sun

College of Sciences Posters

Protein structure prediction produces atomic models from its amino acid sequence. Three-dimensional structures are important for understanding the function mechanism of proteins. Knowing the structure of a given protein is crucial in drug development design of novel enzymes. AlphaFold2 is a protein structure prediction tool with good performance in recent CASP competitions. Phenix is a tool for determination of a protein structure from a high-resolution 3D molecular image. Recent development of Phenix shows that it is capable to refine predicted models from AlphaFold2, specifically the poorly predicted regions, by incorporating information from the 3D image of the protein. The goal …

Telomerase Activity Enhancement In Saccharomyces Cerevisiae, Mackenzie Neal, Hailey Rietz, Melissa Mefford

2022 Celebration of Student Scholarship - Poster Presentations

Telomeres are located at the ends of eukaryotic linear chromosomes and are composed of repeated nucleotide sequences. One of their main functions is to protect chromosomal ends from being damaged. Telomeres cannot be completely copied during DNA replication so they gradually shorten during each replication cycle in what is known as the “end replication problem”. To counteract this problem, the RNA dependent enzyme complex telomerase works to extend telomeres and help protect the ends of chromosomes. Telomeres and the telomerase enzyme are heavily involved in the aging process and cancer progression. Telomeres gradually shorten with age and eventually become so …

Development Of Anti-Cancer Small Molecules To Inhibit The Base Excision Repair (Ber) Pathway By Binding Apyrimidinic/Apurinic (Ap) Sites In Dna, Ellen S. Ledford, Robert M. Tackett, Elizabeth M. Thomas

2022 Celebration of Student Scholarship - Poster Presentations

Two of the main treatments available to patients with cancer are radiation and chemotherapy which both rely on the ability to induce DNA damage. One reason cancer deaths perpetrate is due to cancer resistance towards current cancer therapies. The Base Excision Repair (BER) pathway is involved in therapeutic resistance by utilizing DNA glycosylases that recognize and remove the damaged DNA base to leave the aldehyde. Apyrimidinic/Apurinic (AP) enzyme 1, APE1, then recognizes the aldehyde to trigger DNA repair. Alkoxyamines function to competitively covalently bind the aldehyde generated from the AP site. Once the aldehyde is covalently bonded with the alkoxyamine, …

Seasonal Abundance And Sex Ratio Of Earwigflies (Merope Tuber) In Eastern Kentucky, Abigal Fagan, Sean O'Keefe

2022 Celebration of Student Scholarship - Poster Presentations

The earwigfly is a rarely seen insect in the family of scorpionflies. There are three species total, one in Brazil, one in Australia, and the third (Merope tuber), widespread throughout the eastern United states. In a 1984 study, Chris Maier collected 69 earwigflies between 1977 and 1982 from New England. Most of these were collected in July using sticky traps. He collected 43 females and 26 males (sex ratio 2.4:1). In a 2014 study, Skvarla, Hartshorn, and Dowling collected 77 earwigflies from Arkansas, mostly in August using malaise traps, pan traps, Lindgren funnels, and pitfall traps. They collected 58 females …

What Majors Are Chosen By Undergraduate Students Leaving Accounting? A Review Of The Literature, Sarah E. Lager, Kimberly Fatten

2022 Celebration of Student Scholarship - Poster Presentations

Undergraduate accounting retention research has focused primarily on why students choose an accounting major, but less is known about what programs students go to when they exit an accounting major.

Quantifying Ant Populations To Measure Biodiversity In Morehead, Ky, Alyssa Turner, Jude Hall, Tayla Lee, Charles Lydeard, Sean O'Keefe

2022 Celebration of Student Scholarship - Poster Presentations

To effectively conduct conservation efforts, we can use biodiversity to assess the condition of our environment. Biodiversity has been commonly defined as the variety and variability among living organisms within an area. When our ecosystems are at their best, they clean water, purify air, maintain soil, regulate climate, recycle nutrients, and provide food. Everything within an ecosystem is interdependent, so biodiversity is an important factor and indicator of environmental health. Indicators help us to measure and monitor pressures or threats in land and water use, habitat loss or invasive species, the state of species and ecosystems, the conservation response, and …

Economic Feasibility Of Rainwater Harvesting:A Case Study At Morehead State University, Riley Q. Williams, Vijay Subramaniam

2022 Celebration of Student Scholarship - Poster Presentations

Rainwater harvesting (RWH) was primarily considered as a source for freshwater supply or a conservation practice for over coming water shortages in drought prone areas. By retaining storm-water run-off for on site use, harvesting systems reduce the runoff volumes and pollutant masses entering waterways. Consequently, using roof-top RWH as a best management practice has been encouraged by many state and local governments. Some of the most interesting aspects of RWH are the methods of capture, storage, and the use of this natural resource at the place it occurs.

Genes Repressed After Dna Damage In Acinetobacter Baumannii Are Co-Regulated By Umudab And Ddrr, Mollee Flannigan, Xiaohong Li, Eric Rouchka, Deborah Cook, Janeele Hare

2022 Celebration of Student Scholarship - Poster Presentations

UmuDAb and DdrR coregulate error-prone polymerases in the multi-drug resistant opportunistic pathogen, Acinetobacter baumannii, by repressing polymerase expression until after DNA damage. New evidence indicates that these proteins may also regulate other genes that are repressed following DNA damage. We performed an in silico analysis of RNA-Seq data from wild-type, ddrR, and umuDAb mutant strains to examine the expression levels of genes repressed after DNA damage. We used two different algorithms to analyze Cuffnorm- and HTSeq normalized gene counts. This analysis revealed nineteen (CuffDiff) or twenty-nine (DESeq2) genes repressed in wild-type cells that were derepressed after DNA damage in either …

Analytical Method Development For The Analysis Of E-Liquids, Calista Dean, Charlotte Gabbard, Emma Schmittzehe

2022 Celebration of Student Scholarship - Poster Presentations

Electronic cigarettes are one of the most commonly used methods of nicotine delivery, especially among the adolescent population. Due to the lack of regulation in manufacturer labeling of refillable nicotine solutions (e-liquids), the actual concentration of nicotine and other additives is variable as compared to the reported values. Misreporting of nicotine content is a contributor in the development of nicotine dependency and potentially tobacco product dependency. The objective of this research is to develop reliable analytical methods to study the variations in nicotine levels in e-liquids, and to identify and quantify other potentially harmful additives in e-liquids. In this research …

Identification Of Messenger Molecule For Immuno-Antibacterial Infection Drug Discovery With The Usage Of Bio-Ai, Minqiao (Lisa) Jin

Bioengineering Master's Theses

Facing the danger of Gram-positive bacterial infection, mammalian cells have their defense line. A functional proteomics study aided with Deep Learning indicates the direct interaction between collagen V from mammalian cell membrane and Sortase A which catalyzes the display of virulence factors on the gram positive bacteria. And CHO cell proteomes conducted with the use artificial intelligence via Tensorflow and found that the identified anti collagen V binds to Sortase A of S. aureus and regulates its monomer-homodimer equilibrium, which is responsible for the bacterial cell wall sorting activity. Our results suggest that the collagen V plays an instrumental role …

Engineering Stealthy Exosomes For Drug Delivery, Chris Olson, Mika Domingo

Bioengineering Senior Theses

Exosomes, a class of extracellular vesicles (EVs) are emerging as a new scientific and therapeutic tool for imaging, disease diagnostics, and drug delivery. Exosomes' excellent biological properties make them a strong candidate for surface modification as well as exogenous drug loading. While exosomes have excellent natural biocompatibility properties as well as low immunogenicity, they are still eliminated in the patient’s body through the immune system, mainly through phagocytosis by macrophages. In this report, we aim to aid exosomes in avoiding this elimination method by modifying them through surface display with antiphagocytic factors. We will use CD24 and CD47 “don't eat …

Accelerating Peripheral Nerve Regeneration Using Electrical Stimulation Of Selected Power Spectral Densities, Wei-Ming Yu, Madelyn Mccullen, Vincent Chiun-Fan Chen

Engineering Science Faculty Publications

Peripheral nerve injuries are common consequences of extremity trauma or chronic compression with a prevalence of 43.8 per 1 million people (on average) reported in the United States annually, accompanied by a yearly increase in cost of care. Patients suffering from these injuries require surgical procedures and rehabilitative strategies to reinforce their extensive recovery. Several studies have found that the application of electrical stimulation can accelerate peripheral nerve regeneration, thus shortening the time of peripheral nerve growth and reducing the cost of care (Willand et al., 2016). The electrical stimulation paradigms that effectively enhanced functional recovery in most studies employed …

Healthy Hive: A Beehive Health Management Tool, Kwadwo Amoako

Electrical and Computer Engineering Senior Theses

Honeybees are important pollinators and are essential in the preservation of ecosystems as well as agricultural production. They are crucial to the pollination of 85% of the world’s food crops. However, honeybee populations have been in decline over the past decade, due to external environmental conditions such as diseases and pests (invasive varroa mite), pollution (insecticide), or inadequate forage/poor nutrition. These conditions can have a severe impact on the health of hives. This project will serve as a tool for the monitoring system of tracking the activities of honeybees entering and exiting their beehive. The health condition of the bee …

Dynamics And Simulations Of Discretized Caputo-Conformable Fractional-Order Lotka–Volterra Models, Yousef Feras, Semmar Billel, Al Nasr Kamal

Computer Science Faculty Research

In this article, a prey–predator system is considered in Caputo-conformable fractional-order derivatives. First, a discretization process, making use of the piecewise-constant approximation, is performed to secure discrete-time versions of the two fractional-order systems. Local dynamic behaviors of the two discretized fractional-order systems are investigated. Numerical simulations are executed to assert the outcome of the current work. Finally, a discussion is conducted to compare the impacts of the Caputo and conformable fractional derivatives on the discretized model.

Impulse, Spring 2022, University Marketing And Communications, Jerome J. Lohr College Of Engineering

Impulse (Jerome J. Lohr College of Engineering Publication)

2 | Ahmed Leads Construction and Operations Management
4 | Faculty News
8 | Kelley Steps Aside After 44 Years
12 | Boggs Retires From Mechanical Engineering
14 | Looking At Covid-19 Numbers
16 | Students Help College of Nursing
18 | Midwest Applied Materials Symposium Debuts
20 | Fourth Data Science Event Takes Place
24 | College Achievements
26 | Hard Work Pays Off
28 | Spotlight Shines on Students
30 | Mentoring Program Takes Off
32 | Robotics Club Busy With Activities
34 | The Numbers
36 | Brown, Sieve Honored
38 | Alumna Leads NASA Division
40 …

Directed 3d Chemotaxis Of Cancer Cells In Response To Oscillating Chemical Gradients Using A Microfluidic Device, Trevor R. Thrasher

Honors Capstones

No abstract provided.

Measuring Volatile Emissions From Moss Gametophytes: A Review Of Methodologies And New Applications, Danlyn L. Brennan, Leslie M. Kollar, Scott Kiel, Timea Deakova, Aurélie Laguerre, Stuart F. Mcdaniel, Sarah Eppley, Elliott T. Gall, Todd Rosenstiel

Center for Life in Extreme Environments Publications

Mosses inhabit nearly all terrestrial ecosystems and engage in important interactions with nitrogen-fixing microbes, sperm-dispersing arthropods, and other plants. It is hypothesized that these interactions could be mediated by biogenic volatile organic compounds (BVOCs). Moss BVOCs may play fundamental roles in influencing local ecologies, such as biosphere–atmosphere–hydrosphere communications, physiological and evolutionary dynamics, plant–microbe interactions, and gametophyte stress physiology. Further progress in quantifying the composition, magnitude, and variability of moss BVOC emissions, and their response to environmental drivers and metabolic requirements, is limited by methodological and analytical challenges. We review several sampling techniques with various analytical approaches and describe best practices …

Development Of A Ppg Sensor Array As A Wearable Device For Monitoring Cardiovascular Metrics, Jose Ignacio Rodriguez-Labra

Masters Theses

Wearable devices with integrated sensors for tracking human vitals are widely used for a variety of applications, including exercise, wellness, and health monitoring. Photoplethysmography (PPG) sensors use pulse oximetry to measure pulse rate, cardiac cycle, oxygen saturation, and blood flow by passing a light beam of variable wavelength through the skin and measuring its reflection. A multi-channel PPG wearable system was developed to include multiple nodes of pulse oximeters, each capable of using different wavelengths of light. The system uses sensor fusion along with a machine learning model to perform feature extraction of relevant cardiovascular metrics across multiple pulse oximeters …

Genes Involved In Cell Division In Acinetobacter Baumannii Are Coregulated By Umudab And Ddrr, Belinda Candra, Xiaohong Li, Eric Rouchka, Deborah Cook, Janelle Hare

2022 Celebration of Student Scholarship - Poster Presentations

In the presence of DNA damage, the multi-drug resistant bacteria Acinetobacter baumannii employs the proteins UmuDAb and DdrR to repress the expression of error-prone polymerases.

Quadratic Neural Network Architecture As Evaluated Relative To Conventional Neural Network Architecture, Reid Taylor

Senior Theses

Current work in the field of deep learning and neural networks revolves around several variations of the same mathematical model for associative learning. These variations, while significant and exceptionally applicable in the real world, fail to push the limits of modern computational prowess. This research does just that: by leveraging high order tensors in place of 2nd order tensors, quadratic neural networks can be developed and can allow for substantially more complex machine learning models which allow for self-interactions of collected and analyzed data. This research shows the theorization and development of mathematical model necessary for such an idea to …

Concurrent Identification, Characterization, And Reconstruction Of Protein Structure And Mixed-Mode Dynamics From Rdc Data Using Redcraft, Hanin Rafiq Omar

Theses and Dissertations

A complete understanding of the structure-function relationship of proteins requires an analysis of their dynamic behaviors and the static structure. However, all current approaches to studying dynamics in proteins have their shortcomings. A conceptually attractive and alternative approach simultaneously characterizes a protein's structure and its intrinsic dynamics⁠. Ideally, such an approach could solely rely on RDC data-carrying both structural and dynamical information. The major bottleneck in utilizing RDC data in recent years has been attributed to a lack of RDC analysis tools capable of extracting the pertinent information embedded within this complex data source.

Here we present a comprehensive strategy …

Three-Dimensional Numerical Modeling Of Flow Hydrodynamics And Cohesive Sediment Transport In Enid Lake, Mississippi, Xiaobo Chao, A. K. M. Azad Hossain, Mohammad Z. Al-Hamdan, Yafei Jia, James V. Cizdziel

Faculty and Student Publications

Enid Lake is one of the largest reservoirs located in Yazoo River Basin, the largest basin in the state of Mississippi. The lake was impounded by Enid Dam on the Yocona River in Yalobusha County and covers an area of 30 square kilometers. It provides significant natural and recreational resources. The soils in this region are highly erodible, resulting in a large amount of fine-grained cohesive sediment discharged into the lake. In this study, a 3D numerical model was developed to simulate the free surface hydrodynamics and transportation of cohesive sediment with a median diameter of 0.0025 to 0.003 mm …

Activated Sludge Foaming Descriptors And Correlation To Microbial Community Composition, Grace K. Scarim

Master's Theses (2009 -)

Activated sludge is a conventional treatment process for biochemical oxygen demand (BOD) and total suspended solids (TSS) removal at water resource recovery facilities (WRRFs). A common operational issue that arises is activated sludge foaming on top of the aeration basin and secondary clarifier. Foaming events in activated sludge may lead to decreased treatment efficiency, maintenance issues, and potential environmental health risks. Stable foaming events are caused by biological and chemical drivers (i.e., microbes and surfactants) during the aeration process. However, foaming events are difficult to predict and quantify. This research developed an inexpensive and easy-to-use method to quantify foaming potential …

Impact Of Corrosion Inhibitors And Metals On Antibiotic Resistance In Drinking Water Distribution Systems, Lee Kimbell

Dissertations (1934 -)

Drinking water distribution systems are important for transporting clean drinking water. Antibiotic resistant bacteria (ARB) and antibiotic resistance genes (ARGs) in drinking water may pose risks to human and environmental health. Aging infrastructure such as metal pipes can experience significant corrosion, which can have impacts on the chemical and microbial water quality inside of drinking water systems. Chemicals are commonly added to drinking water to prevent corrosion (i.e., corrosion inhibitors). The metal pipe materials and corrosion inhibitors containing metals may have impacts on the bacteria and ARGs in drinking water systems. This research investigated the presence of ARGs in real-world …

Evaluation Of Shoulder Complex Kinematics Pre And Post Rotator Cuff Repair And Effects Of Altering Rotation Sequence, Alyssa Joy Schnorenberg

Dissertations (1934 -)

Approximately 25% of adults in the United States will sustain a rotator cuff tear in their lifetime. Rotator cuff tears can impede one's ability to perform activities of daily living and maintain functional independence. Nearly 300,000 rotator cuff repair surgeries are performed annually and aim to decrease pain, increase range of motion, and enable return to the workforce.The goal of postoperative rehabilitation is to allow healing of the repaired cuff while restoring function and range of motion, but it is demanding and may require a year. However, most rehabilitation protocols are based solely on clinical experience rather than on evidence-based …

The Traveling Salesman Problem: An Analysis And Comparison Of Metaheuristics And Algorithms, Mason Helmick

Senior Honors Theses

One of the most investigated topics in operations research is the Traveling Salesman Problem (TSP) and the algorithms that can be used to solve it. Despite its relatively simple formulation, its computational difficulty keeps it and potential solution methods at the forefront of current research. This paper defines and analyzes numerous proposed solutions to the TSP in order to facilitate understanding of the problem. Additionally, the efficiencies of different heuristics are studied and compared to the aforementioned algorithms’ accuracy, as a quick algorithm is often formulated at the expense of an exact solution.

Reflection Types And Students’ Viewing Of Feedback In A First-Year Engineering Course Using Standards-Based Grading, Heidi A. Diefes-Dux, Laura M. Cruz Castro

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Background: Feedback is one of the most powerful and essential tools for learning and assessment, particularly when it provides the information necessary to close an existing gap between actual and reference levels of performance. The literature on feedback has primarily focused on addressing strategies for providing effective feedback rather than aspects of students’ readiness to engage with feedback. Purpose/Hypothesis: This study investigated whether reflection, as a routine pedagogical intervention grounded in self-regulated learning theory, promotes the frequency with which students view feedback. Design/Method: A quasi-experimental design was employed to examine the relationship between the use of four different reflection types, …

The Tin Man Needs A Heart: A Proposed Framework For The Regulation Of Bioprinted Organs, Linda Foit

Fordham Law Review

Each day, seventeen people die in the United States while waiting for an organ transplant. At least part of this need could be met by bioprinting, a technology that allows the on-demand production of custom-sized organs from a patient’s own cells. The field of bioprinting is progressing rapidly: the first bioprinted organs have already entered the clinic. Yet, developers of bioprinted organs face significant uncertainty as to how their potentially lifesaving products will be regulated—and by which government agency. Such regulatory uncertainty has the potential to decrease investment and stifle innovation in this promising technological field. This Note examines how …