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Optimizing Crispr/Cas9-Mediated Knockdown Of Angptl3 In Liver Cell Lines And Mouse Hepatocytes, Meredith Reeves

All Theses

Familial hypercholesterolemia (FH) is a genetic condition characterized by elevated levels of low-density lipoprotein cholesterol (LDL-C) that leads to an increased risk of developing cardiac disease early in life (Shah et al., 2020). Current treatments such as statins and PCSK9 inhibitors have helped lower LDL-C levels, however they require repeated administration every 4-6 weeks to remain effective (Raal et al., 2018). Angiopoietin-like 3 (ANGPTL3) is an inhibitor of plasma lipid metabolism that has become a promising molecular target for the treatment of FH. Individuals with non-functional copies of ANGPTL3 demonstrate low levels of plasma LDL-C and triglycerides, indicating a protective …

Optofluidic Droplet Lasers Enabled By Fluorescence Resonance Energy Transfer, Fariba Kenarangi

Electrical Engineering Dissertations - Archive

ABSTRACT: Over the past decade, optofluidic lasers have gained much popularity owing to their unique optical and mechanical properties. Optofluidic lasers are flexible, reconfigurable, easy to fabricate and their emission spectrum can be dynamically tuned. One type of these lasers with high Q-factors, is optofluidic droplet laser, which is a miniaturized ring resonator consists of liquid gain medium and optical microcavity made of two immiscible liquid phases. As light couples into the cavity, the whispering gallery mode will be guided around the concaved closed loop surface and it will be constantly reflected owing to total internal reflection. Due to its …

Interactions Of Vortices With Differing Creation Mechanisms In The Wake Of A Normal Flat Plate-Delta Flat Plate Couple: A Numerical Study., Cheruvateth Vivek Nair

Mechanical and Aerospace Engineering Dissertations - Archive

ABSTRACT: Fluid flow is characterized by the presence of various coherent vortical structures such as vortex tubes, vortex streets, and eddies. When these vortical structures interact with each other or other distinct mechanisms in a fluid, they form a chaotic region of mixing where a large transfer of mass and energy is observed; This phenomenon is called a vortex interaction and is commonly observed in nature across large Reynolds number regimes. In engineering, vortex interactions are an important topic of research in areas such as mixing, flow induced vibrations, propulsion, aerodynamics, convection, heat island effects, hydrodynamics, and atmospheric Sciences, to …

Novel Nanoparticles To Stimulate Therapeutic Angiogenesis For The Treatment Of Peripheral Arterial Diseases, Tam Phung Nguyen

Bioengineering Dissertations - Archive

Peripheral arterial disease (PAD) is a severe impairment of arterial vessels resulting in obstruction of normal blood flow in the legs, leading to acute or chronic lower limb ischemia and subsequently high morbidity and mortality rates. Common treatments for PAD, such as medications and surgical revascularization, have several limitations. For instance, medications used to lower cholesterol, reduce high blood pressure, control blood sugar, prevent blood clots, and reduce/delay symptoms such as leg pains. Still, they cannot treat the established disease directly and often cause side-effects, including bleeding, headache, and diarrhea. Meanwhile, many elderly PAD patients cannot undergo surgical options. Therefore, …

Engineering Elastic And Functional Electrospun Nano-Fibers For Potential Heart Repair, Alan Mark Taylor

Bioengineering Dissertations - Archive

In the United States and around the world, cardiovascular disease (CVD) has been one of the main causes of death since 1921, responsible for approximately 1 in 4 deaths domestically. Myocardial infarction (MI), or commonly called a heart attack, is a subset of CVD that often ends in heart failure (HF). Cardiac cells are incapable of regeneration, leading to severe impacts on quality of life. Current treatments for MI fail to target left ventricular remodeling and therefore do little to address the progression towards HF. Cardiac scaffolds for tissue engineering (TE) are a prominent solution in recent research. Developing various …

Advancing The Radiation Oncology Clinic With Motion Management And Automatic Treatment Planning, Damon Anton Sprouts

Bioengineering Dissertations - Archive

The leading cause of premature death (death under the age of 70) is cancer. The top five cancers for both male and female are: lung, colorectum, pancreas, breast cancer, and prostate. In 2020 there was an estimated 19.3 million new cases with an estimated 9.9 million deaths. The cancer burden is expected to grow to 28.4 million by the year 2040. Surgery, chemotherapy, and radiotherapy are the three pillars in the modern clinic for cancer treatment. In radiotherapy, ionizing radiation particles can travel through the patient body, deposit energy along the way and damage the DNA Structure. There needs to …

Multisorb: A Low-Cost Approach To Remove Multiple Metals And Organics From Leachate And Wastewater, Mithila Chakraborty

Civil Engineering Dissertations - Archive

ABSTRACT: Landfill leachates are considered now as a reusable source of water. Even irrigation projects are now considering use of non-traditional water sources, including treated wastewater, to augment supplies. Treated wastewater or leachate, however, may still contain many inorganic and organic pollutants of emerging concern, because traditional wastewater treatment plants are unable to remove all of them. Adsorbents have been demonstrated to effectively remove heavy metals and recalcitrant organics from leachate/wastewater more effectively, at lower cost, and using less chemicals and energy than many competing techniques. A single adsorbent with one pre-treatment, however, is not effective for simultaneously removing different …

Modeling Hierarchical Porous Electrodes With Tailored Anisotropic Structure, Debanjan Sarker

All Theses

Modern electric vehicles and consumer electronics applications demand high specific energy from Li-ion batteries, which can be charged and discharged faster. In the search for high specific energy, researchers have tried to make thicker battery electrodes which contain a greater proportion of active material. While chemical and structural modification of electrodes can help to increase the electronic conductivity of active material, the microstructure of porous electrodes can be engineered to enhance ion transport for fast charge and discharge. Previous research has shown that macropores introduced by directional freeze tape casting can enhance the performance of thick porous electrodes. For instance, …

Reactive Motion Planning Of Autonomous Vehicles In 3-Dimensional Environments Using Collision And Rendezvous Cones, Kashish Dhal

Mechanical and Aerospace Engineering Dissertations - Archive

This dissertation presents a collision cone/rendezvous cone-based approach for reactive motion planning in three-dimensional dynamic environments. Collision avoidance is fundamental to robot motion planning. In dynamic environments, the path and velocities of obstacles are not known a-priori, and hence it is a common practice to use reactive planners. Reactive planners should be computationally inexpensive because they need to act fast to avoid potential collisions. To reduce the computational load, a majority of motion planning algorithms model the shapes of the robots and obstacles as a circle/sphere. However, when the objects are elongated more in one direction than another, the spherical …

Towards On-Chip Integrated Sensors: Photonic Crystal Optical Sensors And Lasers, Zhonghe Liu

Electrical Engineering Dissertations - Archive

Supervising Professor: Weidong Zhou The field of compact integrated optical platform has developed into a new era in the past two decades. On one hand, the semiconductor optoelectronic devices are becoming smaller and cheaper thanks to the development of complementary metal-oxide semiconductor (CMOS) fabrication technologies; on the other hand, the applications of optoelectronics have been deeply coupled to other fields to realize more complicated implementations. Photonic crystal slab (PCS) structure has been playing an increasingly important role in these optical devices, active or passive, by enabling a variety of light-matter interaction mechanisms. The objective of this dissertation is to investigate …

Lasso Based State Transition Modeling With Interactions In Adaptive Interdisciplinary Pain Management, Amith Viswanatha

Industrial, Manufacturing, and Systems Engineering Dissertations - Archive

The Eugene McDermott Center for Pain Management at the University of Texas Southwestern Medical Center has an interdisciplinary pain management program for chronic pain. This program treats patients with a holistic view of reducing chronic pain and improving their physical, mental, and social well-being through treatment interventions. The development of an adaptive treatment decision tool is main goal of the research project. This program is modeled as a two-stage adaptive treatment decision problem, with state transition models representing the transition of patient state, treatment, and outcome variables from stage 1 to stage 2. Interactions between the patient state and treatments …

Role Of Deep Learning Techniques In Non-Invasive Diagnosis Of Human Diseases., Hisham Abouelseoud Elsayem Abdeltawab

Electronic Theses and Dissertations

Machine learning, a sub-discipline in the domain of artificial intelligence, concentrates on algorithms able to learn and/or adapt their structure (e.g., parameters) based on a set of observed data. The adaptation is performed by optimizing over a cost function. Machine learning obtained a great attention in the biomedical community because it offers a promise for improving sensitivity and/or specificity of detection and diagnosis of diseases. It also can increase objectivity of the decision making, decrease the time and effort on health care professionals during the process of disease detection and diagnosis. The potential impact of machine learning is greater than …

Application Of A 14c-Assay To Assess Methanotrophic Biodegradation Of Tce In Low Ph Groundwater, Evan Groome

All Theses

Current biological strategies for remediating trichloroethylene (TCE) in low pH aquifers (i.e., pH14C-TCE assay was developed to determine pseudo first-order rate constants for the degradation of TCE in microcosms containing soil and groundwater from the Boeing Michigan Aeronautical Research Center (BOMARC) superfund site, where the pH ranges from 4.1 to 4.9. The ¹⁴C-TCE assay was also adapted to calculate soil-normalized rate constants for data from this site, as well as data that Szwast²¹ collected from the Beltsville Agricultural Research Center (BARC). In addition to natural attenuation, biostimulation through amendments of methane and nutrients were also assessed. This treatment …

Impact Of Spray Coating On The Performance Of Hydrophobic Membranes, Dominick J. Maiorano, Hamid Fattahijuybari, David Warsinger

Discovery Undergraduate Interdisciplinary Research Internship

Membrane distillation (MD) is a rapidly emerging water treatment technology used to combat the global water crisis. Membrane pore wetting is a primary barrier to widespread industrial use of MD. The primary causes of membrane wetting are membrane fouling and an exceedance of liquid entry pressure. The development of different types of polymer membranes and the use of pretreatment have led to significant movement towards the prevention of wetting in MD. We sought to take a new approach to combat membrane wetting that involves coating these membranes with hydrophilic chemical compounds, which consequently would decrease their air permeability. Pulling data …

Measurement Of Enantiomer Percentages For Five Monoterpenes From Six Conifer Species By Cartridge-Tube-Based Passive Sampling Adsorption–Thermal Desorption (Ps-Atd), Ying Wang, Wentai Luo, Todd Rosenstiel, James F. Pankow

Civil and Environmental Engineering Faculty Publications and Presentations

Many monoterpenes have at least two different stereochemical forms, and many biosynthetic pathways are known to favor one product over the other(s). A rapid method was developed and used in the determination of the (-/+" role="presentation" style="box-sizing: border-box; border-radius: 0px; display: inline; line-height: normal; word-spacing: normal; overflow-wrap: normal; white-space: nowrap; float: none; direction: ltr; max-width: none; max-height: none; min-width: 0px; min-height: 0px; border: 0px; padding: 0px; margin: 0px; position: relative;">)-enantiomeric distributions for α-pinene, β-pinene, camphene, limonene, and β-phellandrene as emitted by plant material from six conifer species. The six species included the two pine species Pseudotsuga …

Mechanizing The Removal Of Soil Between Peach Trees Planted On Berms, Coleman Scroggs

All Theses

Armillaria root rot (ARR), primarily caused by the soilborne fungus Desarmillaria tabescens, has become the number one cause for peach tree decline in the Southeastern United States. Research has shown that planting peach trees on shallow berms and excavating the soil around the root collar two years after planting lessens the effects of ARR. However, berms make orchard operations such as pruning, thinning, and harvesting more cumbersome and cause cultural concerns as channels of water at their base can lead to erosion and the slope of the berms leads to herbicide and fertilizer runoff. The objective of this research was …

Act5 Eit System : A Multiple-Source Electrical Impedance Tomography System, Omid Rajabi Shishvan

Legacy Theses & Dissertations (2009 - 2024)

This dissertation describes the design and implementation of Adaptive Current Tomograph 5 (ACT5) with a focus on the digital processing and data ow in the instrument. ACT5 is an electrical impedance tomography (EIT) instrument that produces images of the complex impedivity distribution within the body by injecting currents through and measuring voltages on electrodes applied to the skin. ACT5 is a parallel-drive EIT system with a dedicated current source for each electrode, capable of driving all electrodes simultaneously and measuring the induced voltages on them. It can support up to 48 electrodes with a frequency range of 5 kHz to …

Design And Implementation Of A Novel Eit/Ecg System With An Adaptive Current Source Act-5 / Narrative Competence And Cognitive Mapping As A Culturally Sustaining Pedagogy In The Education Of Emergent Bilinguals, Ahmed Abdelwahab

Legacy Theses & Dissertations (2009 - 2024)

Electrical Impedance Tomography (EIT) is a promising medical imaging technique used to detect the body's internal electrical characteristics based on electrical measurements made on its surface. EIT systems, particularly those that use parallel, multiple source architectures, require current sources with very high output impedance. This is a usual challenge for EIT as the practical current sources come with finite output impedance, which is highly degraded as the operation frequency gets wider. To overcome this challenge and maintain the high output impedance requirement, sources often use complex analog circuits which require manual or electronically-controlled adjustments.

Bioleaching Of Arsenic From Agricultural Soils, Zachary Gilstrap

All Theses

Arsenic is a ubiquitous trace metalloid. However, increasing concentrations of arsenic in soil and water is a problem the world faces due to inputs from anthropogenic sources such as mining, milling, agriculture, and coal ash. One method of remediation for these arsenic-contaminated sites is bioleaching. While it is most often used to extract metals from raw ore, it is a viable and environmentally friendly method to remove the same metals from soil. Bioleaching removes metals by cultivating living organisms such as the fungi Aspergillus niger (A.niger) or the bacterium Thiobacillus ferrooxidans (T.ferrooxidans). This research aimed to investigate A. …

A 3d Tissue Engineering Model To Study Mitral Valve Annulus Calcification Under Diabetic Conditions, Erin James

All Theses

The most complex heart valve is the mitral valve (MV). Many pathologies can affect the MV, including stenosis, regurgitation, prolapse, and mitral annulus calcification (MAC). MAC is chronic degeneration of the annulus, which is the fibrous, saddle-shaped “ring” that can contract and relax with the myocardium. The prevalence of MAC is around 15% but increases in patients with other cardiovascular diseases and risk factors. It is also thought to increase in patients with type 2 diabetes, but MAC has not been properly characterized within this population because of confounding factors such as cardiac disease and kidney disease. The goal of …

An Evaluation Of Energy Consumption Comparing Conventional Water Treatment Plants To Microfiltration And Ultrafiltration Water Treatment Plants, Danielle Larsen

All Theses

The use of low-pressure, hollow fiber microfiltration (MF) and ultrafiltration (UF) membranes has become increasingly prominent in drinking water treatment plant configurations over the past 30 years. At the same time, the topics of energy efficiency and sustainability in industry are becoming progressively more important. Although MF and UF are highly successful in filtration, they are generally thought of as significant energy consumers; however, the data have not yet been available to compare multiple plants in various contexts to determine the factors that most affect energy use. This research compares the energy use of MF and UF treatment plants to …

Development Of A New Affinity Membrane For Rapidly Purifying Non-Antibody Proteins, Friendship Edioma

All Theses

This thesis project describes the modification and evaluation of a new affinity membrane for rapid chromatographic purification of non-antibody proteins. The affinity membrane utilizes Im7/CL7 coupling technology developed by Dr. Vassylyev's lab at the University of Alabama Birmingham (UAB), licensed by TriAltus Bioscience. The behavior of the membrane was evaluated using purified CL7-tagged Cas9 as my model protein for static and dynamic binding capacity analysis.

Chapter one provides an overview on biopharmaceutical drug production process development. I discuss how protein drugs are produced, isolated, and purified from the cell supernatant after upstream phases are completed. Despite increasing demands for biologics, …

The Evacuation Simulation Of Wheelchair Users In A Building Fire: An Initial Dynamic Characterization Of Structural Egress Components, Haley Hostetter

All Theses

People with disabilities are one of the most vulnerable groups involved in building fires. According to the U.S. Fire Administration, in the United States alone, an estimated 700 home fires involve people with physical disabilities each year while over 1700 involve those with mental health disorders. Despite this, the current body of literature shows few studies focused on the evacuation of disabled people. This is a direct result of past and present social injustice on people with disabilities and has resulted in high injury and death rates during fires. To combat this, enrich the literature, and improve their experiences in …

Characterization Of Thermal Gelation Properties In Bioresorbable Thermally Activated Hydrogel Polymers For Hernia Surgery Applications, Alexander Mayfield

All Theses

Hydrogel adhesives are a new class of materials with excellent biocompatibility, which makes them very attractive for biomaterial applications. It has been previously shown that Tetronic T1107, a four-arm poly (propylene oxide)-poly (ethylene oxide) (PPO-PEO) block copolymer, is useful as a chemical crosslinking thermo-responsive hydrogel for bioadhesive applications. The end groups of this polymer are modified with acrylate and N-hydroxysuccinimide (NHS) functional groups. The acrylate end group gives the polymer cohesive properties with long-range chemical crosslinking using dithiothreitol (DTT), while the NHS end group gives the polymer adhesive properties through bonding with amines found in organic tissue. It was found …

The Physical Properties And Handling Characteristics Of Pelletized Biomass Materials, Ryan Dean

All Theses

Biomass and feed pellets can be handled and stored using similar equipment used for other bulk materials; however, their unique physical characteristics can lead to handling challenges. Understanding the flow characteristics of these materials is essential for handling and storage system design, but these characteristics are not well defined in less-than-ideal conditions. The overall objective of this study was to evaluate how moisture content and level of fines influence the angle of repose (AoR) of hardwood, pine bedding, and feed pellets along with determining the impacts of displacement on the shear stress of fuel pellets utilizing a modified linear wall …

Modeling And Performance Analysis Of Deb Based Hydrogen Getters, John A. Matteson

Chemical and Biological Engineering ETDs

Hydrogen getter materials are used to irreversibly absorb hydrogen gas in sealed environments. The DEB based getter system, despite finding widespread use in this application, still posses challenges with respect to experimental performance analysis and fundamental understanding of reaction mechanism. The second chapter of this report addresses the experimental challenges of this getter system by demonstrating the utility and experimental strengths of a custom magnetic suspension balance (MSB) based gravimetric sorption system. This apparatus enables performance evaluation in process relevant conditions including dilute mixed gas environments which our results indicate is necessary for accurate performance evaluation of getter systems. Chapter …

Parallel Algorithms For Scalable Graph Mining: Applications On Big Data And Machine Learning, Naw Safrin Sattar

LSU New Orleans Theses and Dissertations

Parallel computing plays a crucial role in processing large-scale graph data. Complex network analysis is an exciting area of research for many applications in different scientific domains e.g., sociology, biology, online media, recommendation systems and many more. Graph mining is an area of interest with diverse problems from different domains of our daily life. Due to the advancement of data and computing technologies, graph data is growing at an enormous rate, for example, the number of links in social networks is growing every millisecond. Machine/Deep learning plays a significant role for technological accomplishments to work with big data in modern …

Modeling The Impact And Accelerating The Process Of Transitioning To A Sustainable Healthy Diet Through Decision Support Systems, Prince Agyemang

Graduate Theses and Dissertations

Food production and consumption are essential in human existence, yet they are implicated in the high occurrences of preventable chronic diseases and environmental degradation. Although healthy food may not necessarily be sustainable and vice versa, there is an opportunity to make our food both healthy and sustainable. Attempts have been made to conceptualize how sustainable healthy food may be produced and consumed; however, available data suggest a rise in the prevalence of health-related and negative environmental consequences of our food supply. Thus, the transition from conceptual frameworks to implementing these concepts has not always been effective. This paper explores the …

The Effect Of Water Management And Ratoon Rice Cropping On Methane Emissions And Harvest Yield In Arkansas, Marguerita Leavitt

Graduate Theses and Dissertations

Sustainable intensification of rice farming is crucial to meeting human food needs while reducing environmental impacts. Rice produces 8% of all anthropogenic CH4, which is a potent greenhouse gas. CH4 emissions can potentially be reduced by cultivation practices that minimize the number of days the fields are saturated, such as dry-seeding instead of water-seeding and irrigation using the alternate wetting and drying (AWD) technique instead of delayed, continuous flooding (DF). Ratoon cropping, wherein a second crop of rice is grown from the harvested stubble of the first crop, can be used to produce additional yield with minimal labor, but may …

Hierarchically Structured Photoelectrodes Via Atomic Layer Deposition, Justin Rowan Reed Demoulpied

Graduate Theses and Dissertations

In the search for a sustainable method to meet increasing energy needs, solar energy emerges as an underutilized, plentiful resource. Solar intermittency and requirements for transportation necessitate storing solar energy in the form of chemical bonds via artificial photosynthesis. Photoelectrochemical (PEC) water splitting generates hydrogen fuel from solar energy and water. A semiconducting material that successfully meets the complex requirements for building an industrially scalable PEC device has yet to emerge. This is leading to a reevaluation of materials previously overlooked within PEC research, mainly materials with limitations such as minimal charge carrier mobility and propensity to corrosion under illumination …