Digital Commons Network^™

Effects Of Feeding Mode On The Performance, Life Span And Greenhouse Gas Emissions Of A Vertical Flow Macrophyte Assisted Vermifilter, Rajneesh Singh, Chittaranjan Ray, Daniel N. Miller, Lisa M. Durso, Yulie Meneses, Shannon Bartelt-Hunt, Matteo D’Alessio

Faculty and Student Publications

This study was conducted to investigate the impact of intermittent feeding on performance, clogging, and gaseous emission on macrophyte assisted vermifiltration (MAVF) based treatment system. Synthetic slaughterhouse wastewater was applied to two different integrated vertical flow based MAVFs. Triplicates were used throughout the study. Eisenia fetida earthworms were added to MAVFs, and Carex muskingmenis plants were planted. Wastewater was applied to the reactors on 1) intermittent (8 h/day) (IMAVF) and 2) continuous (24 h/day) (CMAVF) basis. The average chemical oxygen demand, total nitrogen, and total phosphorous removals achieved by the IMAVF were 80.2 ± 1.6%, 53.9 ± 1.3% and 66.5 …

An Ultrasensitive Bacterial Detection Platform For Culture-Free Diagnosis Of Infections, Xuyang Shi

ETD Archive

The current methods of the diagnosis of bloodstream infections are based on bacterial culture growth, a process that requires considerable time, e.g., 12-16 hours, to obtain a result. This long wait time for the result creates many problems, including the generation of multi-drug resistant organisms (MDROs). At the same time, infected bloodstream usually contains a very low concentration of bacteria, i.e., lower than 5 CFU/mL. The long diagnosis time and the extremely low concentration of bacteria in the infected bloodstream make such infections difficult to diagnose. Here, we demonstrate a culture-free approach for the diagnosis of bloodstream infections using a …

Lipids Affect The Diffusion Of Estradiol In Osteonal Bone, Nicholas Anthony Cimino

ETD Archive

In healthy osteonal bone, embedded osteocytes form an intercellular communication network through overlapping cell membrane extensions, possibly defining an overlooked and clinically relevant lipid-mediated transport pathway for nonpolar molecules. Previous techniques evaluating solute transport in cortical bone limit tissue analysis to microscale areas (less than 1 mm2) using tracers and assumptions that diminish clinical relevance, presenting the need for an improved method to evaluate solute diffusion in macroscale areas (greater than 1 mm2) of osteonal bone. A new diffusion system - constructed of glass and polytetrafluoroethylene - was designed and validated for this purpose, exhibiting minimal adsorption of solutes and …

Interdisciplinary Design Studio: Programming Document Visioning For A Robotic Demonstration, Research, And Engagement Dairy, Steve(N) Hardy, Nate Bicak, Sarah Alduaylij, Noor Al-Maamari, Devyn Beekman, Kelsey Belgum, Lauren Chubb, Nicholas Forte, Mitchell Hill, Joshua Holstein, Dylan Lambe, Phuong Le, Mia Leriger, Elizabeth Loftus, Josh Lorenzen, Megan Lovci, Alex Martino, Zade Miller, Hannah Morgan, Annabelle Nichols, Collin Shearman, Rebecca Sowl, Nalin Theplikhith, Angela Vu, Shaylee Wagner, Ethan Watermeier, Trever Zelenka

Architecture Program: Student Creative Activity

The 2022 COLLABORATE Design Studio brought together students from various disciplines to address a complex, real-world project which required collaborative input from different perspectives. The studio worked to advance the co-creation of knowledge between external stakeholders, students, and instructors. The course was co-taught by faculty from different disciplines, and areas of expertise. During the semester, Nate Bicak and Steven Hardy worked with students from Architecture and Interior Design in collaboration with students in Dr. Tami Brown-Brandl’s students in Biological Systems Engineering and Animal Science to explore the values, spatial qualities, and area requirements of a Robotic Demonstration, Research, and Engagement …

Physical Modeling Of Filament Growth And Resistive Switching In Metal Oxide-Based Rram, Kena Zhang

Material Science and Engineering Dissertations - Archive

Metal oxide-based resistive random-access memories (RRAM) exhibit several excellent performances, such as nanosecond switching speed, large write-erase endurance, and long retention time, and can potentially replace the traditional circuit elements for use as the fundamental units in next-generation hardware deep-learning or neuromorphic systems. The functionality of a metal oxide-based RRAM is attributed to an oxygen vacancy (V_O^(..))-rich conductive filament (CF), which initially forms, and later dissolves or regrows inside the oxide layer during the resistive switching process. However, the complicated interplays among the coexisting chemical, electrical, mechanical, and thermal effects during the formation, growth, and rupture of the CFs make …

An In Vitro 3d Model To Investigate Iol: Posterior Lens Capsule Interactions On Lens Epithelial Cell Responses, Joyita Roy

Bioengineering Dissertations - Archive

ABSTRACT: Posterior Capsule Opacification (PCO) is the most common complication associated with Intraocular Lens (IOL) implantation. The disease is caused by the infiltration and proliferation of Lens Epithelial Cells (LEC) into the interface between the IOL and Posterior Lens Capsule (PLC). The severity of these cell responses depends on a lot of factors like the IOL design, IOL material, and interaction between the IOL and the posterior lens capsule. While some studies have also shown that adsorption of certain proteins present in the PLC, like fibronectin, may affect IOL-induced PCO in the clinical setting, the mechanism governing such interactions is …

Targeted Drug-Loaded Nanoparticle System (Tdnps) To Ameliorate And Treat Idiopathic Lung Fibrosis, Harish Ramachandramoorthy

Bioengineering Dissertations - Archive

Idiopathic pulmonary fibrosis (IPF) is a fatal progressive disease with a dismal prognosis and 3-5 years of survival. Up to date, there are no effective treatments, and a few current therapies that do not ameliorate the pulmonary fibrosis but rather just increase the survival time by 2-3 years. The major factor limiting the treatment is the lack of targeted therapies to deliver the drugs past the mucosa, into the disease microenvironment. To combat this disease, we proposed a localized therapy via our targeted drug-loaded nanoparticles (TDNPs) to effectively deliver drugs and treat lung fibrosis. Our objective was to design TDNPs …

Re-Engineering Cardio-Oncology Testing Using Biomimetic Heart Slice Cultures., Jessica Miller

Electronic Theses and Dissertations

28% of drug withdrawal from the market are due to unforeseen disruptions in cardiomyocyte contractility and electrophysiology. The most commonly used platforms for drug testing are in vivo animal models and in vitro cell culture models. While both have been of paramount importance for the discovery and detection of many cardiotoxicities and mechanisms of action, they lack the ability to model an intact human myocardium. This work aims to establish cardiac tissue slices, which are 300-micron thin tissue sections taken from the left ventricular myocardium, as an alternative platform for cardio-oncology studies, specifically cardiotoxicity testing. Additionally, this work aims to …

Diy Cell Incubator, Hayden James Jeanor

Electrical Engineering

The purpose of creating a cell Incubator is for the development of cell and tissue production in laboratory settings. Large scale research projects and the medical community grow cells for various reasons, including experiments and creating tissue for patients. However, they cannot simply depend on growing cells in a petri dish that sit on a rack at room temperature. To grow heathy cells in the fastest way possible, they use cell incubators. Cell incubators create an atmosphere within the incubation bay that is designed to promote cell growth. The three main components that need to be constantly regulated, using a …

Microcosm Evaluation Of Natural And Biologically-Enhanced Abiotic Transformation Of Chlorinated Ethenes In Low Permeability Formations, Hao Wang

All Dissertations

Trichloroethene (TCE) is one of the most commonly found hazardous compounds at Superfund sites, especially in groundwater. Its volatility and toxicity pose a threat to drinking water safety and human health. In the past decades, research on the fate of TCE in the environment has shifted to contamination of low-permeability formations, for example fractured bedrock aquifers. TCE back-diffusion from low permeability zones and management of persistent TCE groundwater plumes caused by this long-term source zone pose major challenges for remediation.

Monitored natural attenuation (MNA) is a cost-effective remediation strategy that has gradually gained acceptance by regulators. Compared to extensive active …

Design, Fabrication, And Characterization Of Conjugated Polymeric Electrochemical Memristors As Neuromorphic/Integrated Circuits, Benjamin Grant

All Dissertations

Organic materials are promising candidates for future electronic devices compared to the complementing inorganic materials due to their ease of processability, use, and disposal, low cost of fabrication, energy efficiency, and flexible nature toward implementation as flexible and non-conformal devices.With that in mind, electrochemical materials have been widely demonstrated with commercial use as sensors, displays, and a variety of other electronic devices. As Moore's law predicts the increase in the density of transistors on a chip, the requirement to create either smaller transistors or the replacement of the transistor device entirely is apparent. Memory resistors, coined ``memristor", are variable resistive …

Architectural Control Of Mesenchymal Stem Cell Phenotype Through Nuclear Actin, Janet Rubin, Andre J. Van Wijnen, Gunes Uzer

Mechanical and Biomedical Engineering Faculty Publications and Presentations

There is growing appreciation that architectural components of the nucleus regulate gene accessibility by altering chromatin organization. While nuclear membrane connector proteins link the mechanosensitive actin cytoskeleton to the nucleoskeleton, actin’s contribution to the inner architecture of the nucleus remains enigmatic. Control of actin transport into the nucleus, plus the presence of proteins that control actin structure (the actin tool-box) within the nucleus, suggests that nuclear actin may support biomechanical regulation of gene expression. Cellular actin structure is mechanoresponsive: actin cables generated through forces experienced at the plasma membrane transmit force into the nucleus. We posit that dynamic actin remodeling …

Performance Evaluation Of Recycled Plastic Pins In Two Layers To Stabilize Deep Slope Failures, Sachini Madanayake

Civil Engineering Dissertations - Archive

Slope failures are a common occurrence in highway embankments throughout Texas due to the presence of high plastic clays with expansive behavior. Although shallow slope failures are prevalent, slope failures extending deeper into the slope occur due to the development of micro and macro cracks. The cracks facilitate the infiltration of rainwater deeper into the slope over time. Slope failures incur thousands of dollars’ worth of repairs every year for transportation departments across the country. Recycled Plastic Pins (RPP) have been identified as an effective, cost efficient, and sustainable solution to stabilize shallow failures. The resistance of RPP is mobilized …

Increasing Production Of Therapeutic Mabs In Cho Cells Through Genetic Engineering, Charles Barentine

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Between 2014 and 2018, the global market for therapeutic monoclonal antibodies (mAbs) rose from $60 billion to $115.2 billion with a projected value of $300 billion by 2025. These molecules are used to effectively treat some of the most challenging illnesses from auto-immune diseases to cancer. While mAbs are highly valuable with potent applications, their production at scale remains an outstanding challenge. These molecules are largely produced in Chinese Hamster Ovary (CHO) cells that require highly specific conditions to produce a useful product.

Genetic engineering presents one solution to overcome productivity limits. With the advent of CRISPR (clustered regularly interspaced …

Identifying And Minimizing Underspecification In Breast Cancer Subtyping, Jonathan Cheuk-Kiu Tang

Master's Theses

In the realm of biomedical technology, both accuracy and consistency are crucial to the development and deployment of these tools. While accuracy is easy to measure, consistency metrics are not so simple to measure, especially in the scope of biomedicine where prediction consistency can be difficult to achieve. Typically, biomedical datasets contain a significantly larger amount of features compared to the amount of samples, which goes against ordinary data mining practices. As a result, predictive models may fail to find valid pathways for prediction during training on such datasets. This concept is known as underspecification.

Underspecification has been more accepted …

A Design Of A Digital Lockout Tagout System With Machine Learning, Brandon H. Chen

Master's Theses

Lockout Tagout (LOTO) is a safety procedure instated by the Occupational Safety and Health Administration (OSHA) when doing maintenance on dangerous machinery and hazardous power sources. In this procedure, authorized workers shut off the machinery and use physical locks and tags to prevent operation during maintenance. LOTO has been the industry standard for 32 years since it was instantiated, being used in many different industries such as industrial work, mining, and agriculture. However, LOTO is not without its issues. The LOTO procedure requires employees to be trained and is prone to human error. As well, there is a clash between …

A Study On The Effects Of Cementless Total Knee Arthroscopy Implants' Surface Morphology With Finite Element Analysis, Peter Hunt

Master's Theses

Total knee arthroscopy is one of the most performed and most successful orthopedic surgeries, with nearly a million procedures performed in 2020 in the United States alone. Due to changing patient demographics, the use of cementless fixation for implant stability is becoming more prevalent amongst recipients. Cementless implants rely on the surface morphology of a porous coating to bond implant to bone; the quality of this bond is dependent on an interference fit and the roughness, or coefficient of friction, between implant and bone. Stress shielding is a comparison of the properties in implanted bone to natural bone; it is …

Biodegradation Of Methyl Tert-Butyl Ether And Tert-Butyl Alcohol Using Bioaugmentation With Biowish® Aqua, Elizabeth Villanueva

Master's Theses

Aqua, a commercial product manufactured by BiOWiSH® Technologies, was utilized in this research to study its effectiveness to biodegrade methyl tert-butyl ether (MTBE) and tert-butyl alcohol (TBA). Microcosms containing varying concentrations of MTBE and TBA as well as a growth media and mineral salt solution were examined. Analytical instrumentation used in this study included the use of a gas chromatograph-mass spectrometer (GC/MS) to determine concentrations of MTBE and TBA and a spectrophotometer to extrapolate approximate active biomass concentrations in each experiment. Four different environmental conditions were tested for both MTBE and TBA. The environmental conditions tested for each contaminant included: …

Iot-Based Alert System For Geriatric, Vaibhav Meshram Dr, Meghana P V N, R Sahana, Sai Lakshmi Lasya V, Shreya S Reddy

Manipal Journal of Science and Technology

No abstract provided.

In Vitro Bioreactor For Mechanical Control And Characterization Of Tissue Constructs, Samuel Coeyman

All Dissertations

Heart failure (HF) currently affects over 6 million Americans, 50% of whom die within 5 years of their initial diagnosis. A major contributor to the onset of HF is cardiac fibrosis in the myocardium, which arises when fibroblasts (FBs) are activated in response to heightened mechanical stress from overload conditions like hypertension. Activated FBs remodel the extracellular matrix (ECM) and secrete ECM proteins including collagen. FB remodeling has been studied in the past by applying forces and/or deformations to three-dimensional, cell-seeded gels and tissue constructs in vitro. Unfortunately, previous stretching platforms have traditionally not enabled mechanical property assessment to be …

The Rise Of Terrorism In Africa And What It Means For U.S. Policymakers, Bridget Mary Hughes

Master's Theses

This thesis utilizes three case studies to measure the rise of terrorism in Africa. This qualitative data leads the reader to one ultimate question: what does the rise in terrorism in Africa mean for the United States (U.S.) and U.S. policymakers, National Security academia, students in National Security, if anything at all? The answer is: if civilian and military compounds are being targeted by these violent extremists, as these three case studies demonstrate, it is my recommendation that U.S. policymakers cease sending troops to the African theater and, instead, allocate funding to the citizens and nonprofits of the countries whom …

Characterization Of An Amine-Thiol Cosolvent System : Ethylenediamine And Mercaptoethanol, Fernanda Giongo Fernandes

Legacy Theses & Dissertations (2009 - 2024)

Since its introduction, the amine-thiol cosolvent system has been successfully utilized for the deposition of various thin-film devices, but its mechanism of action is still uncertain. Herein, we have attempted to dissect some of the chemical characteristics of amine-thiol cosolvents, with a special interest taken towards a mixture of ethylenediamine (en) and mercaptoethanol (ME). Conductivity was measured for multiple amine-thiol combinations at different ratios to determine extent of ionization in solution, with en-ME having one of the highest solution conductivities. Exposing the solution to air for several days was found to decrease the conductivity of en-ME, indicating the formation of …

Improving Detection And Classification Of Steady-State Visual Evoked Potentials In Brain-Computer Interfaces, Hadi Habibzadeh

Legacy Theses & Dissertations (2009 - 2024)

Brain-computer interfaces (BCIs) provide an alternative communication channel to send computer commands without requiring any voluntary muscle control. They benefit people with limited communication capabilities due to motor function deficits, spinal cord injuries, and neurological disorders. They may also have applications for healthy individuals.

Development Of Nucleic Acid Diagnostics For Targeted And Non-Targeted Biosensing, Christopher William Smith

Legacy Theses & Dissertations (2009 - 2024)

The field of nucleic acid technology is rapidly expanding with new impactful discoveriesbeing made each year. Starting from the discovery of the double-helix structure, cloning, gene editing, polymerase chain reaction (PCR), CRISPR technology, and even the late mRNA vaccines; nucleic acid technology is at the forefront of improving medicine. Nucleic acid technology is extremely versatile due to its easy programmability, automated cheap synthesis, and even its catalog for numerous chemical modifications that can be used to alter structure stability. For example, the number of permutations that can be made with DNA just by altering the code for adenine (A), cytosine …

Building Structures From Mycelium, Noah M. Brown

Physics

The purpose of this project was to develop a method for creating bricks from mycelium and agricultural waste. The bulk of the research happened in two parts, the first part being a period in which different substrates, strains, and methods were used build the bricks. This research took place in San Luis Obispo and was aided by a group of students in PSC392 under Dr. Pete Schwartz in early 2022. After developing a method for constructing these bricks, the research was moved to St. Thomas, Jamaica, in collaboration with an ecovillage called The Source Farm from July to September. The …

Optogenetic Heart Pacing And Heart Arrest In Drosophila Melanogaster Using Integrated Ocm Imaging And Light Stimulation System, Yuxuan Wang

McKelvey School of Engineering Graduate Student Theses & Dissertations

Optogenetics has been widely applied to cardiovascular research using different models. Among them, Drosophila melanogaster (fruit fly) outstands for its similarity of human genes for disease modeling and short life cycle for rapid screening to analyze genetic mechanisms of heart disease. However, most of the current models only allow either activation or inhibition of the heartbeat, which is not sufficient to model complex arrhythmia. Our lab developed a novel Drosophila transgenic model based on a double transgenic line containing two light-sensitive genes, Channelrhodopsin-2 (ChR2) and Halorhodopsin2.0 (NpHR2.0), that enable dual-directional control of the heartbeat rhythm. Real-time optical control of the …

Supporting Us Army Next-Generation Ground Vehicle Simulation Modeling Using An Integrated Ontology Suite, Edward Louis

All Theses

The development of the US Army’s next-generation of ground vehicle systems is supported by simulations that predict vehicle performance and inform engineering design choices in the design and acquisition process. In this paper, we describe the development of an Integrated Ontology Suite to support modeling and simulation of next generation military ground vehicles. The ontology suite is intended to address model reuse challenges and increase the shared understanding of ground vehicle system simulations. It is intended to function in a Model Library structure that stores, documents, assembles, and executes vehicle simulations. The ontology suite consists of four domain ontologies: Vehicle …

Utilizing Systematic Design And Shape Memory Alloys To Enhance Actuation Of Modular High-Frequency Origami Robots, Jessica M. Den Haese

All Theses

Shape memory alloys (SMAs) describe a group of smart metallic materials that can be deformed by external magnetic, thermal, or mechanical influence and then returned to a predetermined shape through the cycling of temperature or stress. They have several advantages, such as having excellent mechanical properties, being low cost, and being easily manufactured, while also providing a compact size, completely silent operation, high work density, and requiring less maintenance over time. SMAs can undergo sold-to-solid phase transformations, and it is because of these phase transformations that they can experience shape memory effect (SME); or the ability to recover from a …

Using Reinforcement Learning To Improve Network Reliability Through Optimal Resource Allocation, Henley Wells

Graduate Theses and Dissertations

Networks provide a variety of critical services to society (e.g. power grid, telecommunication, water, transportation) but are prone to disruption. With this motivation, we study a sequential decision problem in which an initial network is improved over time (e.g., by adding or increasing the reliability of edges) and rewards are gained over time as a function of the network’s all-terminal reliability. The actions during each time period are limited due to availability of resources such as time, money, or labor. To solve this problem, we utilized a Deep Reinforcement Learning (DRL) approach implemented within OpenAI-Gym using Stable Baselines. A Proximal …

Development Of Optical Coherence Tomography Angiography System For Mouse Eye Imaging And Measurement Of Vasodilation, Yilin Li

McKelvey School of Engineering Graduate Student Theses & Dissertations

Optical coherence tomography angiography (OCTA) is broadly known as a non-invasive technology that allows examining the retinal and choroidal vasculatures, alternative to fluorescein angiography. In this study, we have developed an optical coherence tomography (OCT) system for imaging the mouse eye. We measure the retinal thickness, which is 216.53um. We apply OCTA to characterize the quantitative change in vessel diameter and the perfusion density of the retina after the wild-type mice are administered the adenosine or inhale carbon dioxide. Comparing the images acquired one minute and 5 minutes after the injection or CO₂ inhalation with the baseline, distinct changes …