Digital Commons Network^™

Microcantilever-Based Force Sensing, Control And Imaging, Reza Saeidpourazar

All Dissertations

This dissertation presents a distributed-parameters base modeling framework for microcantilever (MC)-based force sensing and control with applications to nanomanipulation and imaging. Due to the widespread applications of MCs in nanoscale force sensing or atomic force microscopy with nano-Newton to pico-Newton force measurement requirements, precise modeling of the involved MCs is essential. Along this line, a distributed-parameters modeling framework is proposed which is followed by a modified robust controller with perturbation estimation to target the problem of delay in nanoscale imaging and manipulation. It is shown that the proposed nonlinear model-based controller can stabilize such nanomanipulation process in a very short …

Proximate Characterization And Lycopene Determination In Bitter Melon Seed Aril, Luis Raul Jimenez Interiano

Chemical Engineering Undergraduate Honors Theses

Bitter melon aril is the mucilage covering the fruit's seeds which could contain beneficial dietary ingredients. The bright red color of bitter melon aril suggests that it could contain lycopene. The purpose of this study was to determine the proximate composition of bitter melon aril and to explore this material as a potential rich source of lycopene that has health benefits. Bitter melon aril from Sri Lanka, harvested in Arkansas, USA in 2005, 2006, and 2007 was analyzed. An investigation to develop an extraction method for lycopene from bitter melon was performed using liquid-liquid and a polar liquid extraction. The …

Understanding The Effects Of Hydrophobicity For The Detection Of Methylated Dna By Microchannel Electrophoresis, Micah Fritsche

Chemical Engineering Undergraduate Honors Theses

DNA methylation is an epigenetic mechanism used for long - term silencing of gene expression. This silencing of gene expression can be involved in the down regulation of genes involved in apoptosis, which is a process of regulated cell death, and this inability to break down cells can ultimately lead to tumor formation in cancer. Methylation of DNA follows a certain pattern through developmental stages of an individual, and this pattern is normally maintained throughout that individual's life. However, in the DNA of older individuals, this methylation is commonly seen to be altered. This altered pattern of methylation can be …

Development Of An In-Vitro Tissue Engineered Blood Vessel Mimic Using Human Large Vessel Cell Sources, Dimitri E. Delagrammaticas

Master's Theses

Tissue engineering is an emerging field that offers novel and unmatched potential medical therapies and treatments. While the vast aim of tissue engineering endeavors is to provide clinically implantable constructs, secondary applications have been developed to utilize tissue-engineered constructs for in-vitro evaluation of devices and therapies. Specifically, in-vitro blood vessel mimics (BVM) have been developed to create a bench-top blood vessel model using human cells that can be used to test and evaluate vascular disease treatments and intravascular devices. Previous BVM work has used fat derived human microvascular endothelial cells (EC) sodded on an ePTFE scaffold. To create a more …

Surface And Bulk Modification Of Poly(Lactic Acid), Rahul Rasal

All Dissertations

The major drawbacks of PLA are its poor toughness and lack of readily reactable groups. Unfortunately, typical methods of PLA toughening are associated with significant modulus and/or ultimate tensile strength (UTS) loss. The main objective of this research was to toughen PLA, with minimal modulus and/or UTS loss, and introduce reactive groups into the PLA matrix in one step. Initially, this objective was divided into two separate parts: PLA surface modification followed by toughening.
PLA film was solvent cast from chloroform solution and was surface modified using a sequential two-step photografting approach. Benzophenone was photografted onto the film surface in …

Hydrologic Information Systems: Advancing Cyberinfrastructure For Environmental Observatories, Jeffery S. Horsburgh

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Recently, community initiatives have emerged for the establishment of large-scale environmental observatories. Cyberinfrastructure is the backbone upon which these observatories will be built, and scientists' ability to access and use the data collected within observatories to address research questions will depend on the successful implementation of cyberinfrastructure. The research described in this dissertation advances the cyberinfrastructure available for supporting environmental observatories. This has been accomplished through both development of new cyberinfrastructure components as well as through the demonstration and application of existing tools, with a specific focus on point observations data. The cyberinfrastructure that was developed and deployed to support …

Optimal Fractional Order Proportional And Integral Controller For Processes With Random Time Delays, Varsha Bhambhani

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

This thesis developed a new practical tuning method for fractional order proportional and integral controllers (FO-PI / PI^α) for varying time-delay systems like networked control systems (NCS), sensor networks, etc. Based on previously proposed FO-PI controller tuning rules using fractional Ms constrained integral gain optimization (F-MIGO), simultaneous maximization of the jitter margin and integrated time weighted absolute error (ITAE) performance for a set of hundred gain delay time-constant (KLT) systems having different time-constants and time-delay values are achieved. A multi-objective optimization algorithm is used to simultaneously maximize the ITAE factor and jitter margin of the plants at initial …

Controlling Nonspecific Adsorption Of Proteins At Bio-Interfaces For Biosensor And Biomedical Applications, Harshil D. Dhruv

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Partitioning of poly(ethyleneglycol) (PEG) molecules in 2-D and 3-D systems is presented as a self-assembly approach for controlling non-specific adsorption of proteins at interfaces.

Lateral restructuring of multi-component Langmuir monolayers to accommodate adsorbing proteins was investigated as a model 2-D system. Ferritin adsorption to monolayers containing cationic, nonionic, and PEG bearing phospholipids induced protein sized binding pockets surrounded by PEG rich regions. The number, size, and distribution of protein imprint sites were controlled by the molar ratios, miscibility, and lateral mobility of the lipids.

The influence of PEG chain length on the ternary monolayer restructuring and protein distribution was also …

Inducing A Normal Phenotype In Breast Epithelial Cells Using A Three-Dimensional Basement Membrane Extract Culture System: A Study On The Reversion Of Cancer, Ross H. Booth

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Experimentally, traditional developmental models and transgenic animals consistently underscore the importance of studying cell behavior in the correct tissue context. However, live animal experimentation is inherently complex, and systematic assessment of the effects of individual variables, such as cell shape and matrix compliance on cell behavior, is extremely difficult at best. Two-dimensional monolayer culture of key individual cell types has provided abundant, fundamental information on cell response, but cannot be used to show the normal phenotype of breast epithelial cells. Furthermore, their results often fail to translate into in vivo and clinical studies. It has been previously established that normal …

Hydraulics Of Ideal Drip Irrigation Systems, Evan J. Thompson

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The hydraulics of IDEal drip irrigation system components were analyzed under controlled laboratory conditions and the results can be applied to the design of IDEal systems. The hydraulic loss coefficient for the lateral-submain connector valves was determined based on laboratory measurements. It was found that the hydraulic loss due to friction in the lay-flat laterals can be accurately estimated with standard friction loss equations using a smaller effective diameter based on the wall thickness and inlet pressure head. The equivalent length barb loss, expressed as an equivalent length of lateral, was calculated for button emitters, as well as for micro-tubes …

Vascular Injury After Whole Thoracic X-Ray Irradiation In The Rat, S. N. Ghosh, Q. Wu, M. Mäder, B. L. Fish, John E. Moulder, Elizabeth R. Jacobs, Meetha Medhora, Robert C. Molthen

Biomedical Engineering Faculty Research and Publications

Purpose

To study vascular injury after whole thoracic irradiation with single sublethal doses of X-rays in the rat and to develop markers that might predict the severity of injury.

Methods and Materials

Rats that received 5- or 10-Gy thorax-only irradiation and age-matched controls were studied at 3 days, 2 weeks, and 1, 2, 5, and 12 months. Several pulmonary vascular parameters were evaluated, including hemodynamics, vessel density, total lung angiotensin-converting enzyme activity, and right ventricular hypertrophy.

Results

By 1 month, the rats in the 10-Gy group had pulmonary vascular dropout, right ventricular hypertrophy, increased pulmonary vascular resistance, increased dry lung …

Development Of A New Lagrangian Float For Studying Coastal Marine Ecosystems, Alex Schwithal, Chris Roman

Graduate School of Oceanography Faculty Publications

This paper presents an overview and initial testing results for a shallow water Lagrangian float designed to operate in coastal settings. The presented effort addresses the two main characteristics of the shallow coastal environment that preclude the direct of use of many successfully deep water floats, namely the higher variation of water densities near the coast compared with the open ocean and the highly varied bathymetry. Our idea is to develop a high capacity dynamic auto-ballasting system that is able to compensate for the expected seawater density variation over a broad range of water temperatures and salinities while using measurements …

Smallsats, Robert J. Twiggs

Robert "Bob" Twiggs STEM Education Collection

A PowerPoint Presentation created by Professor Bob Twiggs titled "SmallSats" from April 30 2009.

Real-Time Optical Flow Sensor Design And Its Application On Obstacle Detection, Zhaoyi Wei

Theses and Dissertations

Motion is one of the most important features describing an image sequence. Motion estimation has been widely applied in structure from motion, vision-based navigation and many other fields. However, real-time motion estimation remains a challenge because of its high computational expense. The traditional CPU-based scheme cannot satisfy the power, size and computation requirements in many applications. With the availability of new parallel architectures such as FPGAs and GPUs, applying these new technologies to computer vision tasks such as motion estimation has been an active research field in recent years. In this dissertation, FPGAs have been applied to real-time motion estimation …

Ultrasonic Drug Delivery To Tumors Via Stealth Liposomes, Peter Jones, Ghaleb A. Husseini, William G. Pitt, Beverly L. Roeder

Faculty Publications

Our research develops ultrasonically-controlled drug delivery systems. Previous micellar drug carriers released the drug upon ultrasonication. We have developed a liposomal drug carrier that is even more effective upon insonation in reducing tumors.

A Parallel Molecular Dynamics Program For Simulation Of Water In Ion Channels, Laxmi Mullapudi

Theses and Dissertations

With a modest beginning from developing a model of dynamics of hard liquid spheres (Alder et al., 1957), molecular dynamics (MD) simulations have come to a point where complex biomolecules can be simulated with precision close to reality (Noskov et al., 2007). In this context, a parallel molecular dynamics program for simulation of ion channels associated with cellular membranes has been developed. The parallel MD code developed is simple, efficient, and easily coupled to other codes such as the hybrid molecular dynamics/ brownian dynamics (MD/BD) code developed for the study of protein interactions (Ying et al., 2005). The Atom Decomposition …

Structural And Functional Considerations In The Design Of Collagen-Based Electrospun Scaffolds, Chantal Ayres

Theses and Dissertations

Electrospinning can be used to selectively process a variety of natural and synthetic polymers into highly porous scaffolds composed of nano-to-micron diameter fibers. This process shows great potential as a gateway to the development of physiologically relevant tissue engineering scaffolds. In this study we examine the structural and functional considerations regarding electrospun scaffolds for dermal template applications using novel quantification techniques. In order to characterize scaffold structure, a technique utilizing the fast Fourier transform was developed to systematically quantify fiber alignment and evaluate how different electrospinning parameters impact the structure and material properties of an electrospun scaffold. Gelatin was suspended …

Static And Dynamic Responsive Behavior Of Polyelectrolyte Brushes Under External Electrical Field, Hui Ouyang, Zhenhai Xia, Jiang Zhe

Mechanical Engineering Faculty Research

The static and dynamic behaviors of partially charged and end-grafted polyelectrolyte brushes in response to electric fields were investigated by means of molecular dynamics simulation. The results show that the polymer brushes can be partially or fully stretched by applying an external electric field. Moreover, the brushes can switch reversibly from collapsed to stretched states, fully responding to the AC electric stimuli, and the gating response frequency can reach a few hundred MHz. The effects of the grafting density, the charge fraction of the brushes and the strength of the electric field on the average height of the polymer brushes …

Unified Theory Of Gas Damping Of Flexible Microcantilevers At Low Ambient Pressures, Rahul Bidkar, Ryan Tung, Alina Alexeenko, Sumali Hartono, Arvind Raman

PRISM: NNSA Center for Prediction of Reliability, Integrity and Survivability of Microsystems

Predicting the gas damping of microcantilevers oscillating in different vibration modes in unbounded gas at low pressures is relevant for increasing the sensitivity of microcantilever-based sensors. While existing free-molecular theories are valid only at very high Knudsen numbers, continuum models are valid only at very low Knudsen numbers. We solve the quasisteady Boltzmann equation and compute a closed-form fit for gas damping of rectangular microcantilevers that is valid over four orders of magnitude of Knudsen numbers spanning the free-molecular, the transition, and the low pressure slip flow regimes. Experiments are performed using silicon microcantilevers under controlled pressures to validate the …

A Numerical Approach For Predicting The Impact Of Vascular Architecture On Blood Flow During Ischemic Repair, Stephanie Ann Wood, Shilpi Ghosh, Kevin Jones, Gabriel Gruionu, Trevor R. Cardinals

Biomedical Engineering

Abstract of conference presentation.

Functional Vasodilation Following Murine Chronic Hindlimb Ischemia, Matthew David Yocum, Trevor R. Cardinal

Biomedical Engineering

Abstract of conference presentation.

Impedance Spectroscopy Of Zebrafish Sperm, Robert Egnatchik

Honors Capstones

No abstract provided.

2009 - The Fourteenth Annual Symposium Of Student Scholars

Symposium of Student Scholars Program Books

The full program book from the Fourteenth Annual Symposium of Student Scholars, held on April 13, 2009. Includes abstracts from the presentations and posters.

Reflection Contributions To The Dispersion Artefact In Ftir Spectra Of Single Biological Cells, Paul Bassan, Hugh Byrne, Joe Lee, Franck Bonnier, Colin Clarke, Paul Dumas, Ehsan Gazi, Michael Brown, Noel Clarke, Peter Gardner

Articles

Fourier transform infrared spectra of a single cell in transflection geometry are seen to vary significantly with position on the cell, showing a distorted derivative-like lineshape in the region of the optically dense nucleus. A similar behaviour is observable in a model system of the protein albumin doped in a potassium bromide disk. It is demonstrated that the spectrum at any point is a weighted sum of the sample reflection and transmission and that the dominance of the reflection spectrum in optically dense regions can account for some of the spectral distortions previously attributed to dispersion artefacts. Rather than being …

Methods For Simultaneous Control Of Lignin Content And Composition, And Cellulose Content In Plants, Vincent Lee C. Chiang, Laigeng Li

Michigan Tech Patents

The present invention relates to a method of concurrently introducing multiple genes into plants and trees is provided. The method includes simultaneous transformation of plants with multiple genes from the phenylpropanoid pathways including 4CL, CAld5H, AldOMT, SAD and CAD genes and combinations thereof to produce various lines of transgenic plants displaying altered agronomic traits. The agronomic traits of the plants are regulated by the orientation of the specific genes and the selected gene combinations, which are incorporated into the plant genome.

Miniaturization Techniques To Realize Very Small Satellites, Robert J. Twiggs

Robert "Bob" Twiggs STEM Education Collection

A PowerPoint presentation created by Professor Bob Twiggs titled "Miniaturization Techniques to Realize Very Small Satellites" from April 6, 2009.

Sustainability And Civil Engineering, Jeremy D. Rodgers

Ohio Valley Regional Student Conference

No abstract provided.

Lower Hobble Creek Ecosystem Flow Recommendations, Melissa Stamp, Thomas Eddie, Darren Olsen, Tyler Allred

All In-stream Flows Material

This report describes the process and products of developing a suite of year-round instream flow recommendations for lower Hobble Creek in Utah County, Utah. This project was undertaken by the Utah Reclamation Mitigation and Conservation Commission (the Commission) as a component of the June Sucker Recovery Implementation Program (JSRIP) 2008 Work Plan (JSRIP 2008). The Commission is a Federal agency established by the Central Utah Project Completion Act (CUPCA [Titles II through VI of Public Law 102-575]). The Commission is responsible for mitigating impacts of the Bonneville Unit of the Central Utah Project (CUP) on fish, wildlife, and related recreation …

Applied Electrokinetics For Tissue Engineering Applications, Stephanie R. Tully-Dartez

Doctoral Dissertations

Tissue engineering could provide an alternative source of transplant tissue regardless of donor supply and with reduced risk of immune rejection. Engineered tissue requires three critical components for successful development: the cells, growth factors, and the scaffold on which they will initially grow. The scaffold acts as a temporary extracellular matrix (ECM) allowing cell attachment and acclimation to the environment prior to synthesis and construction of the cell's native ECM. Prior to cell seeding, the scaffold must be characterized to determine whether or not the pore geometry is conducive to cell implantation. Electrochemical impedance spectroscopy (EIS) provides a unique and …

Impulse, Eric Ebsen, Dan Genzler, Dana Hess, Kyle Johnson

Impulse (Jerome J. Lohr College of Engineering Publication)

College:
[Page] 2 Electrical Engineering & Computer Science Building: Staff took occupancy of the 43,000-square-foot structure during spring break with the public invited to a May 1 dedication.
[Page] 4 Master’s in physics: As the state takes over an underground lab in the Black Hills, SDSU is launching a program to assist students wanting to work there.
[Page] 6 Adding up to success: Math majors find more paths on the track to success and that’s been a prime factor in the multiplication of math majors.
[Page] 8 What happened in New Orleans? Levee expert shares insight on how Katrina claimed …