Digital Commons Network^™

Differential Regulation Of Immature Articular Cartilage Compressive Moduli And Poisson's Ratios By In Vitro Stimulation With Igf-1 And Tgf-Β1, Gregory M. Williams, Kristin J. Dills, Christian R. Flores, Michael E. Stender, Kevin M. Stewart, Lauren M. Nelson, Albert C. Chen, Koichi Masuda, Scott J. Hazelwood, Stephen M. Klisch, Robert L. Sah

Biomedical Engineering

Mechanisms of articular cartilage growth and maturation have been elucidated by studying composition-function dynamics during in vivo development and in vitro culture with stimuli such as insulin-like growth factor-1 (IGF-1) and transforming growth factor-beta 1 (TGF-β1). This study tested the hypothesis that IGF-1 and TGF-β1 regulate immature cartilage compressive moduli and Poisson’s ratios in a manner consistent with known effects on tensile properties. Bovine calf articular cartilage from superficial-articular (S) and middle-growth (M) regions were analyzed fresh or following culture in medium with IGF-1 or TGF-β1. Mechanical properties in confined (CC) and unconfined (UCC) compression, cartilage matrix composition, and explant …

The Relation Between The Arterial Stress And Restenosis Rate After Coronary Stenting, Linxia Gu, Shijia Zhao, Aswini K. Muttyam, James M. Hammel

Department of Mechanical and Materials Engineering: Faculty Publications

Two commercially available stents (the Palmaz–Schatz (PS) and S670 stents) with reported high and low restenosis rates, respectively, have been investigated in this paper. Finite element models simulating the stent, plaque, and artery interactions in 3 mm stenosed right coronary arteries were developed. These models were used to determine the stress field in artery walls after stent implantation. The material properties of porcine arteries were measured and implemented in the numerical models. The stress concentration induced in the artery by the PS stent was found to be more than double that of the S670 stent. It demonstrated a good correlation …

Comparing Implementations Of Magnetic-Resonance-Guided Fluorescence Molecular Tomography For Diagnostic Classification Of Brain Tumors, Scott C. Davis, Kimberley S. Samkoe, Julia A. O’Hara, Summer L. Gibbs-Strauss, Keith D. Paulsen, Brian W. Pogue

Dartmouth Scholarship

Fluorescence molecular tomography (FMT) systems coupled to conventional imaging modalities such as magnetic resonance imaging (MRI) and computed tomography provide unique opportunities to combine data sets and improve image quality and content. Yet, the ideal approach to combine these complementary data is still not obvious. This preclinical study compares several methods for incorporating MRI spatial prior information into FMT imaging algorithms in the context of in vivo tissue diagnosis. Populations of mice inoculated with brain tumors that expressed either high or low levels of epidermal growth factor receptor (EGFR) were imaged using an EGF-bound near-infrared dye and a spectrometer-based MRI-FMT …

Manipulation Of Suspended Single Cells By Microfluidics And Optical Tweezers, Nathalie Neve De Mevergnies, Sean S. Kohles, Shelley R. Winn, Derek C. Tretheway

Mechanical and Materials Engineering Faculty Publications and Presentations

Chondrocytes and osteoblasts experience multiple stresses in vivo. The optimum mechanical conditions for cell health are not fully understood. This paper describes the optical and microfluidic mechanical manipulation of single suspended cells enabled by the μPIVOT, an integrated micron resolution particle image velocimeter (μPIV) and dual optical tweezers instrument (OT). In this study, we examine the viability and trap stiffness of cartilage cells, identify the maximum fluid-induced stresses possible in uniform and extensional flows, and compare the deformation characteristics of bone and muscle cells. These results indicate cell photodamage of chondrocytes is negligible for at least 20 min for laser …

Multi-Frequency Processing For Lumen Enhancement With Wideband Intravascular Ultrasound, Rory A. Carrillo

Master's Theses

The application of high frequency ultrasound is the key to higher resolution intravascular ultrasound (IVUS) images. The need to further improve the IVUS spatial resolution may drive the transducer center frequency even higher than the current 40 MHz range. However, increasing the center frequency may be challenging as it leads to stronger scattering echoes from blood. The high level of blood scattering echoes may obscure the arterial lumen and make image interpretation difficult. Blood backscatter levels increase with transmission center frequency at a much greater rate compared to arterial tissue. These different frequency dependencies provide a potential method to distinguish …

Event Study Method For Validating Agent-Based Trading Simulations, Shih-Fen Cheng

Research Collection School Of Computing and Information Systems

In this paper, we introduce how one can validate an event-centric trading simulation platform that is built with multi-agent technology. The issue of validation is extremely important for agent-based simulations, but unfortunately, so far there is no one universal method that would work in all domains. The primary contribution of this paper is a novel combination of event-centric simulation design and event study approach for market dynamics generation and validation. In our event-centric design, the simulation is progressed by announcing news events that affect market prices. Upon receiving these events, event-aware software agents would adjust their views on the market …

Multiple Trophic Levels Fueled By Recirculation In The Columbia River Plume, Raphael M. Kudela, Alexander R. Horner-Devine, Neil S. Banas, Barbara M. Hickey, Tawnya D. Peterson, Ryan M. Mccabe, Evelyn J. Lessard, Elizabeth R. Frame, Kenneth W. Bruland, David A. Jay, Jay O. Peterson, William T. Peterson, P. Michael Kosro, Sherry L. Palacios, Maeve C. Lohan, Edward P. Dever

Civil and Environmental Engineering Faculty Publications and Presentations

Large rivers represent gateways for the transport of terrigenous and anthropogenic material to the coastal ocean. Here we document a ?700 km2 recirculation or bulge associated with the Columbia River plume that retains recently discharged river water sufficiently to create a regional bioreactor. Fueled by a fluvial nitrate source, this feature stimulated growth across three trophic levels and may buffer this gateway system during periods of increased warming and stratification that lead to decreased ocean productivity, potentially enhancing production at multiple trophic levels and enriching surfacewaters far from the river mouth.

Design Of Novel Drug Delivery System And Optimal Dosage Regimens, Kwang Seok Kim

Dissertations

Three representative drug delivery systems were analyzed to emphasize the roles of mathematical models and computer-aided simulations in pharmaceutical research. In the first project, a protocol was developed so that the optimal regimen, consisting of the intravenous boluses and subsequent infusion of theophylline, could be obtained once information on the pharmacokinetics became available. The method was based on a two-compartment model of the human body. A module was created and posted on a website for free access. The second project dealt with the transdermal heat-assisted delivery of corticosterone. Heat conduction and drug diffusion through the patch and the skin were …

Atomic And Electronic Structure Studies Of Nano-Structured Systems : Carbon And Related Materials, Sumit Saxena

Dissertations

Modeling in the framework of density functional theory has been conducted on carbon nanotubes and graphene nano-structures. The results have been extended to non-carbon systems such as boron nanostructures. Computational studies are complemented by experimental methods to refine the structural models and obtain a better understanding of the electronic structure.

It is observed that the zigzag edged bilayered graphene nanoribbons are highly unstable as compared to their armchair counterparts. A novel approach has been proposed for the patterning of chirality/diameter controlled single walled carbon nanotubes. Nanotube formation is found to be assisted by edge ripples along with the intrinsic edge …

An Adaptive Neuron Circuit For Signal Compression, Sheng Feng Yen, John G. Harris

Electrical and Computer Engineering Faculty Research & Creative Works

We present a low-bandwidth analog circuit for implementing an adaptive biphasic leaky integrate-and-fire neuron. This neuron circuit is targeted for signal compression in neural recording applications. Unlike other adaptive neuron circuits, this adaptive integrate-and-fire neuron supports signal reconstruction with known threshold voltages. Matlab simulations show promising bandwidth reduction comparing to an integrate-and-fire neuron without the adaptive feature. We quantify the circuit performance in terms of the tradeoff between signal reconstruction accuracy and bandwidth. ©2010 IEEE.

A Roof Runoff Strategy And Model For Augmenting Public Water Supply, Robert Carnahan

USF Tampa Graduate Theses and Dissertations

Water is the essential resource that is becoming extremely scarce worldwide. The 21st century will further stress all available water resources through the growth and expansion of developing nations. It is not only the quantity of cheap water that is being depleted, but the quality of these waters is being endangered. Florida is an example where rapid development and an exploding population are competing for shrinking groundwater resources. Current water use does not address the use of alternative supplies and reuses in the United States.

The objective of this research was to determine a strategy for augmenting existing water supplies …

Mean Firing Rate Spike Representations For Speech Recognition, John G. Harris, Yukun Feng

Electrical and Computer Engineering Faculty Research & Creative Works

The nature of spike coding of auditory signals is studied by comparing mean firing rate codes with conventional approaches in speech recognition tasks. Mean firing rate spike representations are problematic since most auditory nerve fibers are saturated at typical conversation levels. However, these problems are eased somewhat when it is considered that there are other nerve fibers (e.g. low spontaneous firing rate fibers) that could efficiently encode the information at each channel of the cochlea. We show that window-based, mean firing rate features can be used with a crude cochlea model to achieve the same level of performance as a …

The Use Of Spike-Based Representations For Hardware Audition Systems, Shih Chii Liu, Nima Mesgarani, John Harris, Hynek Hermansky

Electrical and Computer Engineering Faculty Research & Creative Works

Humans are able to process speech and other sounds effectively in adverse environments, hearing through noise, reverberation, and interference from other speakers. To date, machines have been unable to match human performance. One profound difference between biological and engineering systems comes at the input stage. In machines, an acoustic signal is typically chopped into short equally spaced segments in time. In biological systems, the cochlea outputs asynchronous spikes that respond in real-time to acoustic inputs. In this paper we describe a spiking cochlea implementation and recent experiments in both speaker and speech recognition that use spikes as input. ©2010 IEEE.

Dynamic Solidification In Nanoconfined Water Films, Peter M. Hoffmann, Shah H. Khan, George Matei, Shivprasad Patil

Publications

Mechanical properties of nanoconfined water layers are still poorly understood and continue to create controversy, despite their importance for biology and nanotechnology. We report on dynamic nanomechanical measurements of water films compressed to a few single molecular layers. We show that the mechanical properties of nanoconfined water layers change significantly with their dynamic state. In particular, we observed a sharp transition from viscous to elastic response even at extremely slow compression rates, indicating that mechanical relaxation times increase dramatically once water is compressed to less than 3–4 molecular layers.

Evaluating Effectiveness Of Best Management Practices To Control Accelerated Sedimentation Of The Morro Bay Estuary, Misgana K. Muleta

Civil and Environmental Engineering

The Morro Bay estuary, located on the central Coast of California approximately half way between Los Angeles and San Francisco, is one of the most important wetlands on the west Coast as it supports wide variety of habitats including numerous sensitive and endangered plant and animal species. Various studies have identified accelerated erosion and subsequent sedimentation as a major threat for sustainability of the bay. Watershed disturbances caused by agricultural activities are believed to be one of the major causes of the accelerated erosion and sedimentation. More than 200 conservation practices have been installed in the watershed since the mid-1990 …

Improved Pcr Design For Mouse Dna By Training Finite State Machines, Salik R. Yadav, Steven M. Corns

Engineering Management and Systems Engineering Faculty Research & Creative Works

This project presents an updated method for classification of polymerase chain reaction primers in mice using finite state classifiers. This is done to compensate for many lab, organism and chemical specific factors that are costly. using Finite State Classifiers can help decrease the number of primers that fail to amplify correctly. for training these classifiers, five different evolutionary algorithms that use an incremental fitness reward are used. Variations to the number of generations and the values in the fitness reward are examined, and the resulting designs are presented. by controlling the fitness reward correctly, there is a potential to develop …

Vector Quantization Based On Steady-State Memetic Algorithm, Chien-Min Ou

Journal of Marine Science and Technology–Taiwan

A novel memetic algorithm (MA) for the design of vector quantizers (VQs) is presented in this paper. The algorithm uses steady-state genetic algorithm (GA) for the global search and K-means algorithm for the local improvement. As compared with the usual MA using the generational GA for global search, the proposed MA dramatically reduces the computational time for VQ training. In addition, it attains a near global optimal solution, and its performance is insensitive to the selection of initial codewords. Numerical results show that it can save more than 70% of computation time while maintaining a comparable performance as previous MA

In Vitro Evaluation Of Mechanical Heart Valve Performance Using A Novel Test Chamber In An Automated Mock Circulatory Loop, Antonio Walker

Theses and Dissertations

Valvular heart disease (VHD) continues to have significant effects on many people’s lives, with numbers expected to grow tremendously over the next few years. Individuals suffering from severe valvular heart disease usually require a heart valve transplant in order to restore the native valve’s vital unidirectional flow regulation. Therefore, artificial heart valve (AHV) research is of very high importance. Previous research studies have provided substantial input in the realm of AHV design relative to durability, thrombotic and hemolytic potential, and overall functionality. However, modifications continue to be warranted due to limitations in the accuracy and time efficiency of the in …

Rapid Cell Extraction In Aqueous Two-Phase Microdroplet Systems, Kalpana Vijayakumar, Shelly Gulati, Andrew J. De Mello, Joshua B. Edel

All Faculty Articles - School of Engineering and Computer Science

Distinguishing specific cells is an essential technique in cell research and clinical diagnostics. We report a novel method to passively isolate and extract cells in a microfluidic device. We utilise a droplet-based microfluidic system to generate an aqueous two phase system in which aqueous droplets consist of two phases in the form of a double emulsion. Specifically, we generate PEG droplets that completely encapsulate DEX droplets within a microfluidic channel. Target cells can be introduced directly into the droplets and driven to partition to the more favourable phase, whilst still being contained within the aqueous droplet. Human T lymphoma cells, …

Multi-Axis Planning System (Maps) For Hybrid Laser Metal Deposition Processes, Frank W. Liou, Jianzhong Ruan, Todd E. Sparks

Mechanical and Aerospace Engineering Faculty Research & Creative Works

This paper summarizes the research and development of a Multi-Axis Planning System (MAPS) for hybrid laser metal deposition processes. The project goal is to enable the current direct metal deposition systems to fully control and utilize multi-axis capability to make complex parts. MAPS allows fully automated process planning for multi-axis layered manufacturing to control direct metal deposition machines for automated fabrication. Such a capability will lead to dramatic reductions in lead time and manufacturing costs for high-value, low-volume components with high performance material. The overall approach, slicing algorithm, machine simulation for planning validation, and the planning results will be presented.

Low-Loss Hollow Waveguide Platforms For Optical Sensing And Manipulation, Evan J. Lunt

Theses and Dissertations

This dissertation presents a method for fabricating integrated hollow and solid optical waveguides on planar substrates. These waveguides are antiresonant reflecting optical waveguides (ARROWs), where high-index cladding layers confine light to hollow cores through optical interference. Hollow waveguides that can be filled with liquids or gases are an important new building block for creating highly-integrated optical sensors. The method developed for fabricating these integrated waveguides employs standard processes and materials used in the microelectronics industry, allowing for parallel, low-cost fabrication. Dielectric cladding layers are deposited on a silicon wafer using plasma-enhanced chemical vapor deposition (PECVD). After the lower cladding layers …

Selective Laser Sintering Of 13-93 Bioactive Glass, Krishna C. R. Kolan, Ming-Chuan Leu, Greg Hilmas, Mariano Garcia Velez

Mechanical and Aerospace Engineering Faculty Research & Creative Works

Bioactive glasses are more promising than biopolymers in fabricating scaffolds for bone tissue repair because they convert to hydroxyapatite, when implanted in vivo. Both direct and indirect selective laser sintering (SLS) methods of 13-93 bioactive glass were considered in this research to study the feasibility of fabricating scaffolds for bone repair applications. Stearic acid was used as the binder in the indirect method to fabricate the scaffolds. The green scaffolds underwent binder burnout and sintering at various soaking conditions between 675⁰C and 700⁰C, achieving a maximum compressive strength of 23.6 MPa, which is higher than that of the human cancellous …

Freeze Extrusion Fabrication Of 13-93 Bioactive Glass Scaffolds For Bone Repair, Tieshu Huang, Nikhil D. Doiphode, M. N. Rahaman, Ming-Chuan Leu, B. Sonny Bal, D. E. Day

Materials Science and Engineering Faculty Research & Creative Works

There is an increasing demand for synthetic scaffolds with the requisite biocompatibility, internal architecture, and mechanical properties for the bone repair and regeneration. In this work, scaffolds of a silicate bioactive glass (13-93) were prepared by a freeze extrusion fabrication (FEF) method and evaluated in vitro for potential applications in bone repair and regeneration. The process parameters for FEF production of scaffolds with the requisite microstructural characteristics, as well as the mechanical and cell culture response of the scaffolds were evaluated. After binder burnout and sintering (60 min at 700°C), the scaffolds consisted of a dense glass network with interpenetrating …

Computer Aided Contour Profiling Of High Strength Deposits, Sriram Praneeth Isanaka, Amar Bala Sridhar, Frank W. Liou, Joseph William Newkirk

Mechanical and Aerospace Engineering Faculty Research & Creative Works

Additive manufacturing processes suffer from the effect of ripples, edge rounding and surface variations. To reduce their effect, ideal process parameters for the laser deposition process were investigated. Also, a new method was identified to analyze deposits by accurately plotting their contours. This was achieved through point cloud data of the deposits generated using coordinate measurement and 3D scanning. Curve fitting was performed on the data in Matlab to generate the contours of the deposit. The intercept values, heights, and contact angle of the curves give an indication of the uniformity of deposits and aid in reducing defects.

An Adaptive Grid-Based All Hexahedral Meshing Algorithm Based On 2-Refinement, Jared D. Edgel

Theses and Dissertations

Adaptive all-hexahedral meshing algorithms have many desirable features. These algorithms provide a mesh that is efficient for analysis by providing a high element density in specific locations, such as areas of high stress gradient or high curvature and reduced mesh density in other areas of less importance. In addition, inside-out hexahedral grid based schemes, using Cartesian structured grids for the base mesh, have shown great promise in accommodating automatic all-hexahedral algorithms. In these algorithms mesh refinement is generally used to capture geometric features. Unfortunately, most adaptive mesh generation algorithms employ a 3-refinement method. This method, although easy to employ, provides …

Development Of Pyrf-Based Genetic System For Targeted Gene Deletion In Clostridium Thermocellum And Creation Of A Pta Mutant, Shital A. Tripathi, Daniel G. Olson, D. Aaron Argyros, Bethany B. Miller, Trisha F. Barrett, Daniel M. Murphy, Jesse D. Mccool, Anne K. Warner, Vineet B. Rajgarhia, Lee R. Lynd, David A. Hogsett, Nicky C. Caiazza

Dartmouth Scholarship

We report development of a genetic system for making targeted gene knockouts in Clostridium thermocellum, a thermophilic anaerobic bacterium that rapidly solubilizes cellulose. A toxic uracil analog, 5-fluoroorotic acid (5-FOA), was used to select for deletion of the pyrF gene. The ΔpyrF strain is a uracil auxotroph that could be restored to a prototroph via ectopic expression of pyrF from a plasmid, providing a positive genetic selection. Furthermore, 5-FOA was used to select against plasmid-expressed pyrF, creating a negative selection for plasmid loss. This technology was used to delete a gene involved in organic acid production, namely pta, which encodes …

Equilibrium Studies For The Removal Of Basic Dye By Sunflower Seed Husk (Helianthus Annuus), Siew-Teng Ong, Pei-Sin Keng, Siew-Ling Lee, Ming-How Leong, Yung-Tse Hung

Civil and Environmental Engineering Faculty Publications

The sorption characteristics of sunflower (Helianthus annuus) seed husk (SSH) to remove Methylene Blue (MB) from aqueous solution under batch conditions have been investigated. Sorption of MB was found to be pH, concentration and agitation dependent. The kinetics of sorption was rapid with 80% sorption taking place within the first 60 min regardless of its initial concentration. The fast attainment of equilibrium implying the biosorbent could be suitable for continuous flow system. Using the pseudosecond order kinetics model, the predictive model for MB sorbed at time t (qt) with the initial concentration of (Co) is given by qt = Cot/[0.0607Co …

The Other Gulf Oil Crisis - In Southwest Asia, Adam Liska

Department of Agricultural and Biological Systems Engineering: Faculty Publications

The oil spill in the Gulf of Mexico is a rude reminder of the risks inherent in an economy dependent upon petroleum. But there's a quieter crisis in the other gulf - the Persian Gulf - that should call Americans' attention to the even more severe consequences of relying upon imported oil. The costs of using the military to protect the transport of oil from the most turbulent part of the world should convince our country's policymakers to increase investments in researching and developing affordable, American-made clean-burning alternative fuels. But, first, we have to understand the real causes, costs and …

Metallic Nanoparticle On Micro Ring Resonator For Bio Optical Detection And Sensing, Ali Haddadpour, Yasha Yi

Publications and Research

We have numerically investigated the unique effects of metallic nanoparticle on the ring resonator, especially multiple Au nanoparticles on the micro ring resonator with the 4-port configuration on chip. For the Au nanoparticle, because it has smaller real refractive index than air and large absorption refractive index, we found that there is a blue shift for the ring resonance wavelength, instead of red shift normally observed for dielectric nanoparticles. The drop port intensity is strongly dependent on both number and size of nanoparticles, while relatively independent on position of nanoparticles. The correlation between the penetration depth of Au and the …

Corrosion Dynamics Of Cobalt-Chromium Alloy F-75 Powder, Tedman Tong

Master's Theses

The increasing usage of metal-on-metal joint replacements consisting of a cobalt-chromium-molybdenum alloy requires increasing concern regarding the inevitable generation of metallic wear debris. Patients with these joint replacements exhibit elevated concentrations of cobalt and chromium ions within their serum, blood and urine. The presence of these metal ions suggests the potential for bodily damage and indicates corrosive processes are acting upon wear debris.

To understand the behavior of these corrosive processes, powders of cobalt-chromium-molybdenum alloy F-75 were studied. Four powder sizes (44, 74, 105, and 420 µm diameter) were subjected to Hank’s Balanced Salt Solution (HBSS) for a 42 day …