Digital Commons Network^™

Application Of Microbial Fuel Cells In A Forested Wetland, Jianing Dai

All Theses

ABSTRACT
Electricity can be generated from forest residues, especially the organic matter from litter fall, in freshwater forested wetland environments with a microbial fuel cell (MFC) system. Electricity generation efficiency was examined for cypress, tupelo, and pine litter extracts, using dual-chamber reactors. The maximum power density generated with cypress extracts was on the level of 320 mW/m2, higher than tupelo (230 mW/m2), and pine (210 mW/m2). The efficacy of cypress extracts was also higher than tupelo and
pine, demonstrating that organic matter from cypress litter is a preferred substrate for electricity generation with MFCs. Meanwhile, low aromaticity of the substrate …

Electrochemical Capacitance Measurements To Study Molecular Surface Interactions, Nrutya Madduri

All Theses

The behavior of biological molecules such as proteins at the electrode/electrolyte has been of considerable interest for the development of biosensors. Several investigative techniques including Potentiometry, Voltammetry, Amperometry, and Electrochemical Impedance Spectroscopy are being employed to study and analyze these molecular surface interactions. Investigative techniques such as cyclic voltammetry involve the application of a large potential to probe the electrode characteristics and capture the bulk membranous events to obtain the required measurements. This study looks to achieve two major objectives through the use of a novel technique, namely, the double layer capacitive method: 1) to analyze the electrochemical behavior of …

Photocatalytic Reactor Using Tio2 Thin Film On Quartz Tubes, Ram Mahipal Kouda

Graduate Theses and Dissertations

Photocatalysis process, as an environmental application is an advanced oxidation process with tremendous potential in the near future. Previously many researchers, have conducted photocatalysis in different reactor configuration using TiO2 slurry and thin film, using a UV light source, the constraints for these reactors are the need for the removal of TiO2 particles at the outlet stream in a slurry reactor and poor environment for the efficient use of TiO2 film using UV light, as the UV light penetration depth in water is about an inch.

Taking all this into consideration, we propose a design for reactor with efficient contact …

Physiochemical And Nanomanipulation Studies Of Carbon Nanomaterials, Siva Naga Sandeep Chalamalasetty

Graduate Theses and Dissertations

Carbon nanomaterials are, without a doubt, one of man's wonder creations. Though these nanomaterials are a very recent trend, extraordinary electromechanical properties and the light weightiness of these nanomaterials attracted the attention of researchers. Although vast research has been done since the start of the US nanotechnology initiative, much effort was in the area of synthesis and characterization of the nanomaterials. However, most of the traditional macroscopic material's theories fail at the nanoscale level, and since the material properties are dependent on size and structure at nanoscale level, the behavior of the carbon nanomaterials in different environments needs attention. High …

Development Of A Microfluidic Device Coupled To Microdialysis Sampling For The Pre-Concentration Of Cytokines, Randy Francisco Espinal Cabrera

Graduate Theses and Dissertations

A proof-of-concept microfluidic device combined with heparin-immobilized magnetic beads was created to concentrate cytokine proteins collected from microdialysis samples. Cytokines are known to be related to several diseases such as cancer, and Parkinson's diseases, so to be able to develop more effective diseases treatments their interactions have to be well understood. Amine-functionalized polystyrene and carboxyl-functionalized magnetic microspheres of ~6.0 ìm in diameter were used to immobilize heparin. The amount of heparin immobilized on polystyrene beads was 5.82 x 10-8 ± 0.36 x 10-8 M per 1.0 x 106 beads and for magnetic beads was 0.64 x 10-8 ± 0.01 x …

Aptamer-Based Spr Biosensor For Detection Of Avian Influenza Virus, Hua Bai

Graduate Theses and Dissertations

Rapid and specific detection of avian influenza (AI) virus is urgently needed with the concerns over the outbreaks of highly pathogenic H5N1 avian influenza in animal and human infection. Aptamers are artificial oligonucleic acid that can specifically bind to target molecules. They show comparable affinity for target virus and better thermal stability than monoclonal antibodies. Those advantages make aptamers promising candidates in diagnostic and detection applications. The goal of this research was to use DNA&ndashaptamer as the specific recognition element in a portable surface plasmon resonance (SPR) biosensor for detection of AI H5N1 virus in poultry.

A SPR biosensor was …

Electrospun Polycaprolactone Nanofiber Scaffolds For Tissue Engineering, Andreas Haukas

Graduate Theses and Dissertations

Stem cell and tissue engineering offer us with a unique opportunity to research and develop new therapies for treating various diseases that are otherwise incurable using traditional medicines. However, development of these new therapies replies upon the establishment of in vitro cell culture and differentiation systems that mimic in vivo microenvironments required for cell-cell and cell-ECM interaction. The development of these cell culture systems depends upon the identification of appropriate biomaterials and cell sources. Biomaterials should be carefully selected and fabricated into scaffolds for supporting cell growth and differentiation. In this study, we explored the fabrication of 3D electrospun nanofiber …

Sediment Removal From The San Gabriel Mountains, Mary C. Ferguson

Pitzer Senior Theses

The issue of sediment removal from the San Gabriel Mountains has been a complex issue that has created problems with beach replenishment, habitat destruction and the need to spend millions of dollars at regular intervals to avoid safety hazards. Most recently 11 acres of riparian habitat, including 179 oaks and 70 sycamores, were removed for sediment placement. Other sites including Hahamongna Watershed Park and La Tuna Canyon also face a similar fate. This thesis questions: How did we get to this point of destroying habitat to dump sediment which is viewed as waste product? What are the barriers for creating …

Integration Of Water-Column And Benthic Processes And Their Effect On Dissolved Oxygen Fluctuations In Small Northern Utah Streams, Ruba A. Mohamed

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Dissolved oxygen (DO) is an important element for aquatic organisms as it is used for respiration by most organisms. Certain concentrations of DO are required in aquatic ecosystems depending on the type and needs of the aquatic organisms. In general, DO concentrations less than 5 mg/L stress many types of aquatic organisms including fishes. In order to maintain DO concentration above the stress level, respiration is one of the factors that should be maintained by controlling the excess growth of aquatic plants. Many factors cause the outbreak of plant growth, including abundance of nutrients and presence of sun light. In …

Chitosan Reduces Water Solubility Of Phosphorus In Poultry Litter, Iain M. Bailey

Biological and Agricultural Engineering Undergraduate Honors Theses

The addition of chemical amendments to land applied poultry litter is paramount to the maintenance of acceptable phosphorus (P) and nitrogen (N) levels in stormwater runoff from pasture. Chitin is the chief component of shrimp shells, and together with its more processed form chitosan, may show promising P, N, and metal ion chelation characteristics by virtue of its chemical structure and unique charge distribution. The objective of this study was to measure the effects of aluminum sulfate (alum, Al2(SO4)3) on aqueous solubility of P and ammonia (NH3) release against those of chitin and three varieties of chitosan in poultry litter. …

Kinetics Of Xylose Oligomers Decomposition, Christopher Mcdaniel

Biological and Agricultural Engineering Undergraduate Honors Theses

Hemicellose-derived oligomers are key components in hemicellulose depolymerization into the five-carbon carbohydrate xylose, a key operation in the production of cellulosic biofuels. Unfortunately, hemicellulose oligomers do not only depolymerize directly into xylose, but also into degradation compounds that reduce the overall production of cellulosic biofuels. Because commercially available oligomers are prohibitively expensive, this study was carried out using relatively inexpensive commercial grade xylose. Understanding the degradation of xylose is important for its impact on the economic viability of the monomeric sugars-to-ethanol conversion. From experimental data and ensuing statistical analysis, initial xylose concentrations did not result in different xylose degradation rates, …

Formation Of Embryoid Bodies From Human Embryonic Stem Cells In Defined Medium, Ashley Kiene

Biological and Agricultural Engineering Undergraduate Honors Theses

Embryonic stem cells have the characteristic of self-renewal and the ability to differentiate into tissues from all three germ layers. Many times, the first step in using human embryonic stem cells for regenerative medicine is often initiated by embryoid body (EB) formation. EBs are three-dimensional multicellular aggregates that resemble early post implantation embryos that still maintain the potential to form the three germ layers. Currently, generating EBs from human embryonic stem cells (hESC) that are adapted to feeder-independent medium is difficult, much more so than feeder cultured hESC. Using a chemically defined medium would not only help the application of …

Evaluation Of Biocomposite For Implementation As Prosthetic Socket, Chelsea Long

Biological and Agricultural Engineering Undergraduate Honors Theses

According to the World Health Organization, 0.5% of the population in developing countries is in need of prostheses or orthoses. For this population, obtaining a prosthetic is made difficult due to medical and transportation costs as well as a general lack of area resources. One possible solution to these problems would be to design a prosthetic socket from an inexpensive biodegradable material that could be produced in developing countries. One such material has been developed by Ecovative Design, LLC. Ecovative’s technology grows mycelium, mushroom roots, throughout a plant fiber matrix. Ecovative products are completely biodegradable and made naturally from local …

Study Of The Fundamental Chemical And Physical Interactions Between Chitosan And Hydroxyapatite, Abigail Washispack

Biological and Agricultural Engineering Undergraduate Honors Theses

Hydroxyapatite and chitosan are widely used in biomedical applications due to their biocompatibility and biodegradability. Various studies show that composites made of hydroxyapatite and chitosan exhibit desirable properties for applications in bone tissue engineering. When creating a composite it is important to understand the interfacial chemistry and physical interactions between the composite materials. An understanding of these properties gives more insight into the resulting structure of the composite on multiple levels (nano- micro- macro). This structure affects the chemical, physical, and mechanical properties of the material, thus analysis of these properties gives a better understanding of how a material will …

Structural Dna Origami: Engineering Supermolecular Self-Assembly For Nanodevice Fabrication, Craig Marshal Onodera

Boise State University Theses and Dissertations

Two challenges encountered in nanotechnology are the ability to create nanostructures inexpensively and the ability to arrange nanomaterials with a precision commensurate with their size. In nature, nanostructures are created using a bottom-up approach, whereby molecules hierarchically self-assemble into larger systems. Similarly, structural DNA nanotechnology harnesses the programmability, specificity, and structural integrity of DNA to engineer synthetic, self-assembled materials. For example, during scaffolded DNA origami, a long single stranded DNA polymer is artificially folded into nanostructures using short oligonucleotides. Once folded, two- and three-dimensional nanostructures may be decorated with proteins, metallic nanoparticles, and semiconductor quantum dots. Using gold nanoparticles and …

Sudden Increase And Regrowth Of Fecal Coliforms And Escherichia Coli In Wastewater Biosolids After High Solids Centrifugation, Gordon G. Araujo

Master’s Theses

Treatment plants that operate either thermophilic or mesophilic anaerobic digesters with centrifugal dewatering processes have consistently observed densities of fecal coliform and Escherichia coli, both indicator bacteria, that decrease during digestion but then increase after dewatering and storage. The increases have been characterized as two separate phenomena to explain this observation: 1) “Sudden Increase,” or SI, which is defined as the increase that occurs immediately after dewatering and 2) “regrowth,” which is defined as an increase during storage of cake samples over a period of hours or days. The SI observation appears to be more prevalent with biosolids that are …

Sweetgum Bark: Extraction, Purification, And Determination Of Antioxidant Activity, Shiloh Hurd

Biological and Agricultural Engineering Undergraduate Honors Theses

Sweetgum (Liquidambar styraciflua L.) is a native tree species that grows as a volunteer in the understory of managed pine forests in Arkansas. Sweetgum, as well as other volunteer species, must be removed before the pines can be harvested. Sweetgum wood can be converted to hemicellulosic and cellulosic sugars by dilute acid pretreatment and enzymatic hydrolysis. These wood-derived sugars can then be fermented into cellulosic ethanol. Usually, the bark is not deconstructed into sugars, but transformed into thermal energy via a combustion process. Interestingly, the bark of the sweetgum contains value-added products that could be recovered prior to thermal processing. …

Microencapsulation Of The Non-Steroidal Anti-Inflammatory Drugs Into Biodegradable Polymers Using Supercritical Fluid Technology, Safaa Mohamad Al Mahdi Ali Yousif

Theses

Osteoarthritis is a disease that attacks human bones especially in older people and usually nonsteroidal anti-inflammatory drugs (NSAIDS) are being prescribed for patients with Osteoarthritis. These kinds of drugs usually have low aqueous solubility and in order to have their therapeutic effects, their solubility should be enhanced. The purpose of this study was to find solution for this problem and one way to do that is to reduce the particle size by forming micro particles. In this project, one of these types of drugs ibuprofen (isobutyl-propanoicphenolic Acid) was encapsulated into a polymer (PVP polyvinylpyrrolidone) using supercritical fluid technology (Supercritical CO …

Fluidized-Bed Gasification Of Dairy Manure By Box–Behnken Design, Hanjing Wu, Milford A. Hanna, David D. Jones

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Application of excessive animal manure to the land may cause some environmental problems such as eutrophication of surface waters, degradation of ground water quality, and threats to human health. This paper reports an experimental study on the technology of biomass gasification to treat animal waste by analyzing the effects of key operating parameters on gasification. In this research, dairy manure from the University of Nebraska dairy farm was first collected and dried, and then gasified in a fluidized-bed, laboratory-scale gasifier to generate syngas. The effects of three parameters, namely temperature, steam to biomass ratio (SBR) and the equivalence ratio (ER), …

Mapping Spatially Interpolated Precipitation, Reference Evapotranspiration, Actual Crop Evapotranspiration, And Net Irrigation Requirements In Nebraska: Part I. Precipitation And Reference Evapotranspiration, Vivek Sharma, Suat Irmak

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Precipitation and reference evapotranspiration are two important variables in hydrologic analyses, agricultural crop production, determining actual crop evapotranspiration and irrigation water requirements, and irrigation management. Both variables vary in space and time, and the weather networks that measure or quantify and report both variables are too sparse for practical applications by water resources planners, managers, and irrigators. Long-term (1986- 2009) average annual (January to December), seasonal (growing season, May to September), and monthly (May, June, July, August, and September) precipitation and Penman-Monteith-estimated alfalfa-reference evapotranspiration (ET_ref) were spatially interpolated and mapped for all 93 counties in Nebraska using the …

Nutrient Losses In Runoff From Feedlot Surfaces As Affected By Unconsolidated Surface Materials, John E. Gilley, Jason R. Vogel, Roger A. Eigenberg, David B. Marx, Brian L. Woodbury

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Beef cattle feedlots contain unconsolidated surface materials (loose manure pack) that accumulate during a feeding cycle. The effects of varying amounts of unconsolidated surface materials on runoff nutrient losses are not well understood. The objectives of this study were to (1) compare runoff nutrient losses from feedlot surfaces containing varying amounts of unconsolidated surface materials, (2) determine if differences in runoff nutrient losses exist among rainfall simulation runs, (3) relate runoff nutrient losses to selected feedlot soil characteristics, and (4) identify the effects of varying runoff rate on nutrient loss rates from feedlot surfaces. This study was conducted on 0.75 …

Novel Phantoms And Post-Processing For Diffusion Spectrum Imaging, Vaibhav Juneja

Dissertations and Theses (Open Access)

High Angular Resolution Diffusion Imaging (HARDI) techniques, including Diffusion Spectrum Imaging (DSI), have been proposed to resolve crossing and other complex fiber architecture in the human brain white matter. In these methods, directional information of diffusion is inferred from the peaks in the orientation distribution function (ODF). Extensive studies using histology on macaque brain, cat cerebellum, rat hippocampus and optic tracts, and bovine tongue are qualitatively in agreement with the DSI-derived ODFs and tractography. However, there are only two studies in the literature which validated the DSI results using physical phantoms and both these studies were not performed on a …

Characterization And Optimization Of Solution-Derived Chalcogenide Glass Thin Films, Jacklyn Wilkinson

All Theses

Chalcogenide glasses have many unique properties that allow them to be used in a variety of optical applications. Infrared transparency allows them to be used in sensors for molecules that have 'fingerprints' in the 2-25 μm range. By producing amorphous thin films of these materials, they can be incorporated into chemical sensors as planar waveguides and resonators. The goal of this work was to fabricate and characterize solution-derived chalcogenide thin films for use in chemical sensors with a source wavelength between 3-3.5 μm.
The structural and optical properties of the parent bulk glass were characterized and used as reference for …

Remote Detection Of Ephemeral Wetlands In Mid- Atlantic Coastal Plain Ecoregions: Lidar And High-Throughput Computing, Paul Leonard

All Theses

Ephemeral wetlands are ecologically important freshwater ecosystems that occur frequently throughout the Atlantic coastal plain ecoregions of North America. Despite the growing consensus of their importance and imperilment, these systems historically have not been a national conservation priority. They are often cryptic on the landscape and methods to detect ephemeral wetlands remotely have been ineffective at the landscape scales necessary for conservation planning and resource management. Therefore, this study fills information gaps by employing high-resolution light detection and ranging (LiDAR) data to create local relief models that elucidate small localized changes in concavity. Relief models were then processed with local …

Applying Chaotic Advection To Rheology: An In Situ Structuring Rheometer, Ramaswami Subrahmanian

All Dissertations

A prototype In Situ Structuring Rheometer (ISSR) was designed and implemented to study changes in shear viscosity of polymer blends and composites while processing them in such a way as to control the evolution of microstructure. The ISSR is based on a regime of fluid mechanics known as chaotic advection, in which simple time-periodic flow fields can cause fluid particles to move chaotically. Chaotic advection is also the basis of Smart Blending, a technology employed to process polymer blends having a variety of morphologies at a fixed composition, and polymer composites in which the additives have been arranged into layered …

Formation Of Molecularly Imprinted Polymer Matrices Using, William Erwin

Chemical Engineering Undergraduate Honors Theses

This study serves as a proof of concept that a poly(acrylonitrile-co-acrylic acid) may be used to form an chiral selective polymer membrane for discrimination of L-tryptophan from racemic tryptophan. Due to the unavailability of necessary equipment, a polymer membrane was not tested, rather, two experiments were run in conjunction; (1) A membrane of L-tryptophan imprinted P(AN-co-AA) was cast and tested on a scanning electron microscope to determine the membrane density, and (2) Imprinted polymer was ground and tested for absorption of L-Tryptophan by finding circular dichroism and concentration for different samples. It was found that it is feasible that P(AN-co-AA) …

Green Ro Pretreatment, Elizabeth Fullerton

Chemical Engineering Undergraduate Honors Theses

The use of sea water desalination to produce potable water for maritime vessels is essential for eliminating storage of potable water. Reverse osmosis (RO) is the technology of choice for desalination purposes; however, raw seawater must undergo pretreatment to avoid rapid fouling and plugging of RO membranes and modules. Current pretreatment technology is effective in the open ocean but in coastal regions involves frequent replacement of filter cartridges and increased maintenance costs. The standard pretreatment process consists of 20 μm cartridge filters and 3 μm cartridge filters in series. Several technologies were considered as options for improving the current pretreatment …

Hollow Fiber Membranes In Increased Algae Growth For Biobutanol Production, Allison Mcatee

Chemical Engineering Undergraduate Honors Theses

Algae are a unique and remarkable species of plant that is capable of thriving on land deemed useless for the cultivation of food crops. They also breathe in the harmful greenhouse gas, carbon dioxide. As the fastest living organism to complete a life cycle, algae is the single most effective user of carbon dioxide in the world thus making it an important ally for diminishing the threat of global warming. Furthermore, algae are comprised of high concentrations of lipids, rendering it an appealing option for use in biodiesel production, specifically butanol. Increasing the algae’s supply of carbon dioxide gas causes …

Using Capillary Electrophoresis To Detect Amyloid Protein Aggregation, Jennifer Kurtz

Chemical Engineering Undergraduate Honors Theses

Alzheimer’s disease (AD) is the leading cause of dementia accounting for ½ to ¾ of all cases. AD is characterized by a progressive loss of cognitive function over a span of 10 years, ultimately ending in death. The main possible cause of AD being researched is the formation of soluble, oligomer amyloids. Amyloids are proteins that misfold and aggregate in the brain causing potent neurotoxic effects on neurons in the brain. Microchip electrophoresis and capillary electrophoresis are two potential methods of determining the level of amyloid aggregation. In order to detect at physiological concentrations, covalent dyes must be used to …

Investigation Of A Commercial Product (Biowish™) For Nitrogen Management, Eva Lee

Master's Theses

Abstract

Investigation of a Commercial Product (BiOWiSH­­^TM) for Nitrogen Management

Eva Lee

BiOWiSH–Aqua, which has the capability of treating nitrogen from wastewater through bioaugmentation, is a commercial product consisting of a blend of microorganisms developed by BiOWiSH Technologies. A study of the treatment of nitrogen compounds (i.e. , , and ) using Biowish–Aqua was conducted using small scale experiments (flask experiments) and large scale experiments (column reactor experiments). In this work, column reactors were created to test Biowish–Aqua’s nitrogen treating capabilities by providing enough depth to simulate the dissolved oxygen gradient that can be observed in a pond. …