Digital Commons Network^™

Development Of A Pdms Based Micro Total Analysis System For Rapid Biomolecule Detection, Balaji Srinivasan Venkatesh

Graduate Theses and Dissertations

The emerging field of micro total analysis system powered by microfluidics is expected to revolutionize miniaturization and automation for point-of-care-testing systems which require quick, efficient and reproducible results. In the present study, a PDMS based micro total analysis system has been developed for rapid, multi-purpose, impedance based detection of biomolecules. The major components of the micro total analysis system include a micropump, micromixer, magnetic separator and interdigitated electrodes for impedance detection. Three designs of pneumatically actuated PDMS based micropumps were fabricated and tested. Based on the performance test results, one of the micropumps was selected for integration. The experimental results …

An Estimation Of Available Cellulosic Biomass In Ten Counties In Northeastern Arkansas From 1999-2011, Thomas Jenkins

Biological and Agricultural Engineering Undergraduate Honors Theses

The purpose of this research is to explore the possibility of siting a biorefinery that produces cellulosic ethanol in the Delta region of Arkansas, which is the main agricultural area of the state. Four major crops that the state of Arkansas produces on a yearly basis that could potentially be used for cellulosic ethanol production are: corn, rice, sorghum, and soybeans. This research will result in the generation of maps that will be useful in quantifying feedstock produced in the Delta region of Arkansas, which can be used by a biorefinery. An actual site will not be chosen based strictly …

Minimizing Wash Water Usage After Acid Hydrolysis Pretreatment Of Biomass, Noaa Frederick

Biological and Agricultural Engineering Undergraduate Honors Theses

Abstract Dilute acid pretreatment, needed to prepare biomass for saccharification, results in the production of a number of byproducts, which inhibit subsequent enzymatic hydrolysis and fermentation steps. In order to improve saccharification yields in the enzyme hydrolysis step, the pretreated biomass is often rinsed with room temperature water to remove these byproducts. High-density poplar was pretreated with 1% dilute sulfuric acid at 140 ºC for 40 minutes. After pretreatment the biomass was washed with water volumes equal to 0, 1 ½, or 3 times the biomass volume. The rinsed biomass was then enzymatically hydrolyzed and the concentrations of byproducts and …

Structural Characterization Of Engineered Muscular Mimetic, Nadia Bhatti

Biological and Agricultural Engineering Undergraduate Honors Theses

An engineered muscular mimetic (EMM) was synthesized, and its overall structure—porosity, average pore size, and pore alignment—was characterized and compared to actual decellularized skeletal muscle (DSM). The EMM’s porosity was about 20% more and average pore size was about 85% smaller compared to that of the DSM. Unlike the strong horizontal pore alignment in the DSM, no distinct orientation could be identified in the EMM structure.

Scaffolding For Human Pluripotent Stem Cell Lineage Specification, Thanh Dai

Biological and Agricultural Engineering Undergraduate Honors Theses

Current research regarding stem cells is the premise behind this project. These cells have the potential to develop into any kind of cell in the human body. Due to the scarcity of transplantable pancreas and/or pancreatic beta cells, the production of pancreatic insulin-producing cells from human embryonic stem cells (hESCs) has emerged as one of the most attractive cell therapy strategies for insulin deficient diabetes treatment. The aspect of hESC research that the project focused on was the environment or foundation the hESCs grew in. A key step to pancreatic beta cell production from hESC differentiation or growth is establishing …

The Effect Of Cell Density And Cultivation Period On Skeletal Muscle Extracellular Matrix Accumulation, Ryan Yarnall

Biological and Agricultural Engineering Undergraduate Honors Theses

Traumatic skeletal muscle injuries have led to severe disability disallowing one to perform necessary daily tasks. Different methods are under current research to rebuild muscle tissue and have it function properly. One such method is the use of extracellular matrix, or ECM, retrieved from cells to create biological scaffolds providing structure for myoblast cells to grow into tissue. This research holds promise because it utilizes the body’s own machinery, minimizing risk of a foreign body response. To obtain ECM scaffolding, one viable technique involves the cultivation of cells on polyurethane foams to collect and harvest ECM. This research investigates the …

Varying Culture Conditions To Enhance Tissue Formation, Katherine Atkins

Biological and Agricultural Engineering Undergraduate Honors Theses

For severe muscle damage, tissue engineering has become a viable option to culture muscle tissues with the ultimate goal of implantation. However, the tissues are typically cultured on permanent scaffolds which would elicit a foreign body response if placed in the body. To avoid this, biological scaffolds are ideal for tissue culture and limiting any negative reactions. A sacrificial scaffold can be used to gather extracellular matrix (ECM), which can then be used as the primary scaffold for culturing tissues for implantation. To reach the ultimate goal of implanting muscle tissue, an understanding of how to produce ECM on a …

Metal Oxide Nanocomposite Nanoliter Reaction Chamber Fabrication And Applications For Harsh Environment Gas Sensing, Michael C. Briggs

Nanoscale Science & Engineering (discontinued with class year 2014)

In order to perform research at the College of Nanoscale Science and Engineering, funding for the desired research is required. Undergraduates get around that by working under a graduate student or professor who is currently funded. For those who seek funding, they receive it by submitting a grant proposal stating the motivation for research, the background, and previous research done to support this new endeavor, as well as future research plans that will be possible with funding. These grant proposals follow certain formats depending on where the principal investigator is seeking funding. As part of the honors requirement for the …

Advancements Towards Single Site Information Storage And Processing Using Hfo2 Resistive Random Access Memory (Reram), Michael Quinlan Hovish

Nanoscale Science & Engineering (discontinued with class year 2014)

Resistive Random Access Memory (ReRAM) has attracted much attention among researchers due to its fast switching speeds, lower switching voltages, and feasible integration into industry compatible CMOS processing. These characteristics make ReRAM a viable candidate for next-generation Non- Volatile Memory. Transition-Metal-Oxides have been proven to be excellent materials for ReRAM applications. This work investigates the effect of various, post-deposition anneals (PDA) on the switching parameters of Ni/Cu/HfO2/TiN Resistive Memory Devices (RMD). Results are presented in the form of a Small Business Innovation Research (SBIR) grant proposal. The use of the SBIR format emphasizes understanding of the experimental design, commercial viability, …

Neoadjuvant Therapy For Ovarian Cancer Using Bioglycogentm Nanoparticles Sbir Grant Proposal, Alexander E. Talamo

Nanoscale Science & Engineering (discontinued with class year 2014)

Project Summary Technical Abstract This Small Business Innovation Research Phase I project, presented by Talamo Inc., is to test an ovarian cancer therapeutic delivery system, comprised of a nanoscale biocompatible vesicle that carries a bioactive small interfering RNA molecule (siRNA), in vivio in mice. The siRNA has shown to reduce the expression of an enzyme (Sod2) that is highly expressed in multiple ovarian cancer types. The reduced expression of Sod2 will allow the tumor to become increasingly susceptible to chemotherapy agents, while simultaneously diminishing tumor progression. Research has proved that the nanoscale vesicle is non-toxic and in combination with siRNA …

Biomass Gasification: An Alternative Solution To Animal Waste Management, Hanjing Wu

Department of Agricultural and Biological Systems Engineering: Dissertations, Theses, and Student Research

The overall goal of this research was to evaluate gasification of animal waste as an alternative manure management strategy, from the standpoints of syngas production and biochar application.

To meet the overall objective, the thermogravimetric characteristics of dairy manure, as a thermochemical conversion feedstock, were studied firstly. Then, gasification technology was applied to dairy manure and feedlot manure using a fluidized-bed laboratory-scale gasifier. In addition, biochar derived from the feedlot manure was examined for its effects on nutrient leaching as a soil amendment. Finally, a life cycle assessment was conducted to evaluate greenhouse gas emissions of two feedlot manure management …

Soil Organic Carbon Dynamics In Agriculture: Model Development And Application From Daily To Decadal Timescales, Matthew P. Pelton

Department of Agricultural and Biological Systems Engineering: Dissertations, Theses, and Student Research

Soil carbon (C) is the largest terrestrial C pool globally, containing more C than the atmosphere and biosphere. Soil organic carbon (SOC) is a source and sink of CO₂ emissions to and from the atmosphere, thus influencing future climate change. Understanding SOC dynamics is also important for maintaining C stocks to sustain and improve crop yields. An existing model to estimate changes in SOC due to respiration was modified to operate in three computational platforms: MS Excel, MS Excel with Visual Basic for Applications, and supercomputing. This model was validated against CO₂ flux data from a 9-year field …

A Memristor-Based Neuromorphic Computing Application, Adrian Rothenbuhler

Boise State University Theses and Dissertations

Artificial neural networks have recently received renewed interest because of the discovery of the memristor. The memristor is the fourth basic circuit element, hypothesized to exist by Leon Chua in 1971 and physically realized in 2008. The two-terminal device acts like a resistor with memory and is therefore of great interest for use as a synapse in hardware ANNs. Recent advances in memristor technology allowed these devices to migrate from the experimental stage to the application stage.

This Master's thesis presents the development of a threshold logic gate (TLG), which is a special case of an ANN, implemented with discrete …

Highly Sensitive Method For Detecting And Separating Pathogens Using Paramagnetic Particles And A Micro-Fluidic System, William Ryan

Biomedical Engineering Undergraduate Honors Theses

Water is one of the most important compounds on Earth. If a water supply is contaminated with a pathogen, it can have devastating results to an individual or a community. This research is directed at constructing an easily reproducible procedure that could increase the sensitivity of detecting and separating bacteria from the current limits of about 100 cells to 10-20 cells per sample with a ultimate goal of a single cell detection. Here, we report a micro-fluidic system to magnetically capture pathogenic bacteria in water followed by rapid impedance detection using Escherichia coli K-12 as a model bacterium. Paramagnetic beads …

Comparing Decellularized Muscle Tissue With An Engineered Muscle Biomaterial, Katelin Cherry

Biomedical Engineering Undergraduate Honors Theses

Decellularized tissues composed of extracellular matrix are commonly used for tissue transplantations but this method has many limitations. An alternative approach is to create an extracellular matrix by seeding degradable foams with cells, culturing these foams to allow extracellular matrix to be secreted from the cells, then washing away the degradable foam to collect the material that is produced. This study was interested in comparing decellularized skeletal muscle tissue to an engineered biomaterial that was seeded with skeletal muscle myoblast cells. Collagen content of decellularized tissue and engineered biomaterial were quantified using a hydroxyproline assay to compare the two samples. …

Floatovoltaics: Quantifying The Benefits Of A Hydro-Solar Power Fusion, Abe Mckay

Pomona Senior Theses

To slow climate change, humans should take immediate and widespread action. One way to slow climate change is by switching to switch to renewable power plants such as solar fields. Recently, pioneering companies have built solar fields on water bodies. This study found that such a pairing of water and solar could increase production efficiency by 8-10% through panel cooling, save millions of liters of water from evaporation, and produce energy with under-utilized space.

Hydraulic Conditions Required To Not Move Unconsolidated Surface Material Located Within Feedlots, John E. Gilley, Gregory D. Boone, David B. Marx

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Beef cattle feedlots contain unconsolidated surface material that accumulates within feedlot pens during a feeding cycle. Runoff from feedlot surfaces is diverted into settling basins. The storage capacity of the settling basins will be substantially reduced if large quantities of solid material are transported in runoff from the feedlot surfaces. The objective of this study was to identify the hydraulic conditions that will not move unconsolidated surface material located within feedlots in order to minimize sediment transport. Selected sizes and a composite sample of unconsolidated surface material were placed within 0.75 m wide by 4.0 m long metal frames. Flow …

Two-Dimensional Blood Flow Velocity Estimation Using Ultrasound Speckle Pattern Dependence On Scan Direction And A-Line Acquisition Velocity, Tiantian Xu, Gregory R. Bashford

Department of Agricultural and Biological Systems Engineering: Faculty Publications

We have previously investigated the change of apparent lateral speckle size caused by the direction and spatial rate of scanner A-line acquisition (scan velocity). An algorithm which measures the lateral component of blood flow velocity was developed based on the increase in speckle size resulting from relative motion between moving scatterers and the scan velocity. In this paper, the change of the apparent dominant angle of the speckle pattern in a straight vessel was investigated and a new method of two-dimensional blood flow velocity estimation is introduced. Different scan velocities were used for data acquisition from blood flow traveling at …

Modeling The Zimmer Fitmore And Ml Taper Implantation, Tyler Kazuo Franklin

Master's Theses

With more young adults requiring total hip

arthroplasties the need for bone saving implants becomes

more important. The Zimmer Fitmore is a new bone saving

implant that utilizes an implantation technique that

reduces the damage to the muscle tissue allowing for

patients to have a short recovery time as well as a new

design that allows it to rest on the medial cortex. There

has been anecdotal evidence that this device leads to early

revision within six months of implantation due to failures

occurring in the medial cortex. The main goal of this

study was to computationally model the Zimmer …

Delayed Thrombus Resolution And Fibroproliferative Vascular Wound Healing From Deficiency Of Type Iii Collagen: A Paradoxical Mechanism For Tissue Fragility, Amy J. Reid

Dissertations and Theses (Open Access)

Vascular Ehlers-Danlos syndrome is a heritable disease of connective tissue caused by mutations in COL3A1, conferring a tissue deficiency of type III collagen. Cutaneous wounds heal poorly in these patients, and they are susceptible to spontaneous and catastrophic rupture of expansible hollow organs like the gut, uterus, and medium-sized to large arteries, which leads to premature death. Although the predisposition for organ rupture is often attributed to inherent tissue fragility, investigation of arteries from a haploinsufficient Col3a1 mouse model (Col3a1^+/-) demonstrates that mutant arteries withstand even supraphysiologic pressures comparably to wild-type vessels. We hypothesize that injury …

Analysis Of Water Footprint Of A Photobioreactor Microalgae Biofuel 1 Production System From Blue, Green And Lifecycle Perspectives, Jason C. Quinn

Mechanical and Aerospace Engineering Faculty Publications

Microalgae are currently being investigated as a feedstock for the commercial production of transportation fuels, due to their potential scalability and sustainability advantages over conventional feedstocks. The water consumption of microalgae has been postulated to be a resource barrier for large-scale production. This study presents an assessment of the water footprint (WF) of a closed photobioreactor-based biofuel production system, where microalgae cultivation is simulated with geographical and temporal resolution. The assessment focuses on the WF as modeled for four different fuel conversion pathways, and in 10 continental US locations corresponding to high productivity yields. The WF is comprehensively assessed using …

Aqueous Suspensions Of Polymer Coated Magnetite Nanoparticles: Colloidal Stability, Specific Absorption Rate, And Transverse Relaxivity, Steven Saville

All Dissertations

The design, functionalization, characterization, and applications of magnetic nanoparticles have garnered significant interest over the past several decades. While this area has garnered increasing attention, several questions remain unanswered about the stability of these systems and it's influence on their biomedical applications. To help answer these questions about the stability of these, a novel tri(nitroDOPA) terminated polymer based ligand has been developed for the stabilization of magnetite nanoparticles. The synthesis involves a process in which ethylene oxide is polymerized using a trivinyl initiator, modified with carboxylic acid using a free radical addition of mercaptoundecanoic acid, and then functionalized with nitroDOPA …

The Production And Characterization Of A Multi-Functional Fiber-Based Composite For Use In Protective Clothing, Jessica Green

All Dissertations

A fiber-based composite comprised of two functional components which work concurrently to adsorb toxic organic compounds was developed and characterized for use in chemical threat protective clothing. The first functional component, the sorptive layer, consists of a carded nonwoven loaded with adsorptive particles. In this layer, Capillary-Channeled PolymerTM (C-CPTM) fibers were used instead of traditional round fibers since the grooved nature of the C-CPTM fibers enables increased adsorptive particle loading. The species of adsorptive particles investigated, zeolite and modified PS, are considered as a replacement for more commonly used activated carbon spheres. The second functional component, the flow restrictive layer, …

Functional Dissection And Module Swapping Of Fungal Cyclooligomer Depsipeptide Synthetases, Dayu Yu, Fuchao Xu, David Gage, Jixun Zhan

Biological Engineering Faculty Publications

BbBSLS and BbBEAS were dissected and reconstituted in Saccharomyces cerevisiae. The intermodular linker is essential for the reconstitution of the separate modules. Module 1 can be swapped between BbBEAS and BbBSLS, while modules 2 and 3 control the product profiles. BbBSLS is a flexible enzyme that also synthesizes beauvericins.

Investigation Of A Lagrangian-Point Propellant Depot Rendezvous Approach For An Interplanetary Mission To Mars, Joshua W. Ehrlich

Doctoral Dissertations and Master's Theses

This thesis investigates the feasibility of a refueling station for a beyond Earth orbit (BEO) mission. The propellant depot is located at the L₂ -Lagrangian point within the Earth-Moon system. This investigation determines if a refueling mission will reduce propulsive energy requirements necessary for Earth departure of a BEO-vehicle to achieve an interplanetary transfer to Mars. Furthermore, this research presents a trade study which identifies approximate total requirements for a direct and rendezvous mission to Mars as well as the necessary fuel masses to complete each approach. This analysis provides conclusive information in determining the benefits in utilizing a …

Protein Isolation Using Peptoid Based Affinity Chromatography, Megan Dunn

Chemical Engineering Undergraduate Honors Theses

Protein purification is essential for advancements in biotechnology. There are several different methods employed in purifying a particular protein from a complex sample such as a cell lysate. These methods take advantage of differences in the size, charge or binding affinity of the protein. One such method is affinity chromatography which utilizes the binding affinity of a protein toward a certain ligand to purify a protein. This is usually used as a final step to extract the desired protein after the mixture has undergone other purification steps to remove unwanted materials. The goal of this project was to develop a …

Novel Method For Sequential Batch Recovery Of Biomass From A Helical Photobioreactor, Matthew Hurst

Chemical Engineering Undergraduate Honors Theses

This study developed and evaluated a novel method to recover the microalgae species Arthrospira platensis from a closed helical photo bioreactor system. A recovery apparatus was designed and shown to increase recovery by 40% via periodic online recovery runs during exponential growth phase of biomass. Considerations for the apparatus design included the low shear tolerance of the algae and the necessity for a closed system in order to monitor CO2 consumption. The recovery method draws on methods used for large scale recovery as reported in literature by Vonshak and Shimmatsu [1], [2]. This work seeks to improve reactor biomass yield …

Optimal Production Of Biobutanol Via Hydrogenation Of Butyric Acid, Sarad Panta

Chemical Engineering Undergraduate Honors Theses

Butanol is a primary alcohol most commonly used industrially as a solvent. It is largely produced petrochemically using fossil fuel feedstocks. The global butanol market has steadily increased in recent years and is projected to continue increasing at an annual rate of 3.2%. Additionally, the possibility of introducing butanol to the fuel market presents the opportunity to further grow the industry. Because of the increasing demand and its production from fossil fuels, there is a need to manufacture butanol more sustainably and efficiently to help the environment and improve the world economy. Currently, biobutanol is not produced commercially; however, its …

Development Of The Multiple Use Plug Hybrid For Nanosats (Muphyn) Miniature Thruster, Shannon Dean Eilers

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The Multiple Use Plug Hybrid for Nanosats (MUPHyN) prototype thruster incorporates solutions to several major challenges that have traditionally limited the deployment of chemical propulsion systems on small spacecraft. The MUPHyN thruster offers several features that are uniquely suited for small satellite applications. These features include 1) a non-explosive ignition system, 2) non-mechanical thrust vectoring using secondary fluid injection on an aerospike nozzle cooled with the oxidizer flow, 3) a non-toxic, chemically-stable combination of liquid and inert solid propellants, 4) a compact form factor enabled by the direct digital manufacture of the inert solid fuel grain. Hybrid rocket motors provide …

Improving Surrogate Monitoring Techniques For Suspended Sediment, Brant R. Whiting

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The quality of water of our nation’s rivers and streams is important to many vital uses including drinking water treatment, recreation, and the natural environment. Water quality can be severely impacted by the quantity and type of suspended sediment found therein. Because suspended sediment can be associated with many other contaminants that degrade water quality, it is noted as the most common impairment to water quality in the United States. Suspended sediment can cause significant ecological impacts to the chemical and biological characteristics of surface waters. The ability to accurately quantify suspended sediment concentrations at the appropriate time(s) and location(s) …