Digital Commons Network^™

Evaluation Of Triploid Oysters As A Tool To Assess Short- And Long-Term Seafood Contamination Of Oil Spill-Impacted Areas, Martin Scott Miles

LSU Doctoral Dissertations

Many of the organic components of oil have the capacity to persist in the environment, bioaccumulate in tissues, and are toxic to surrounding organisms. Since the early 1970s, environmental assessment programs have utilized bivalves as monitoring tools of chemical contaminants in the marine environment. Because of their sedentary and filter-feeding habit (bioaccumulation), mussels and oysters been employed as sentinel organisms in environmental quality monitoring studies in coastal ecosystems. The use of indigenous (diploid) bivalves for biomonitoring water quality is limited during the summer months due to high stress and changes in biochemical composition induced by reproductive development. In this work …

Polycyclic Aromatic Hydrocarbon Degradation In Tidally Influenced Coastal Marsh Wetland Studied In Laboratory Mesocosm, Doorce S. Batubara

LSU Doctoral Dissertations

The compact mesocosm which was designed for a laboratory scale was used for the kinetic study of refractory organic compounds in a tidally influenced coastal marsh wetland soil. The tidal cycles were controlled pneumatically using an air chamber inside the mesocosm tank. Phenanthrene as the test compound showed that its degradation in the intertidal wetland soil was faster than that in the subtidal wetland soil. Oxygen resupply during the tidal cycles to the intertidal wetland soil would enhance the degradation. Comparison of degradation rates of phenanthrene (Phe), pyrene (Pyr), and benzo[e]pyrene (BeP) were also studied using the mesocosm. The degradation …

The Effects Of Concurrent Driving And In-Vehicle Tasks: A Multivariate Statistical Analysis Of Driver Distraction In A High-Fidelity Driving Simulator, Julius A. Codjoe

LSU Doctoral Dissertations

Distracted driving continues to remain a cause of concern for a number of bodies, including government agencies, traffic safety advocacy groups and law enforcement agencies, because of its traffic safety risks. The driving simulator continues to be popular with researchers in collecting data on performance variables that provide scientific knowledge of the effects of distracted driving. Several of these performance variables can be used to quantify a single distracting effect, resulting in a multivariate dataset. A literature review of related studies revealed that researchers overwhelmingly use univariate (single and multiple) tests to analyze the resulting dataset. Performing multiple univariate tests …

Synthesis And Characterization Of Novel Polyester Scaffolds From Sugarcane Industry By-Products For Use In Skin And Bone Tissue Engineering, Akanksha Kanitkar

LSU Doctoral Dissertations

The aim of this work was to synthesize non-toxic, biodegradable polyesters of aconitic acid, cinnamic acid and glycerol from by-products of the sugarcane industry as scaffolds for skin and bone tissue engineering. Utilizing the by-products, molasses and sugarcane bagasse, not only add value to the cane industry, but also paves path for synthesizing novel bio-based materials from the isolated specialty chemicals. Molasses contain an economically recoverable quantity of aconitic acid and its extraction was studied in detail as a part of this work. The yields of recovered aconitic acid varied from 25–69% depending on the extraction conditions. Under all the …

Image Enhancement Methods And Applications In Computational Photography, Qinchun Qian

LSU Doctoral Dissertations

Computational photography is currently a rapidly developing and cutting-edge topic in applied optics, image sensors and image processing fields to go beyond the limitations of traditional photography. The innovations of computational photography allow the photographer not only merely to take an image, but also, more importantly, to perform computations on the captured image data. Good examples of these innovations include high dynamic range imaging, focus stacking, super-resolution, motion deblurring and so on. Although extensive work has been done to explore image enhancement techniques in each subfield of computational photography, attention has seldom been given to study of the image enhancement …

Less Is More: Long-Term In Vitro Exposure To Low Levels Of Silver Nanoparticles Provides New Insights For Nanomaterial Evaluation, Kristen K. Comfort, Laura K. Braydich-Stolle, Elizabeth I. Maurer, Saber M. Hussain

Chemical and Materials Engineering Faculty Publications

In view of the vast number of new nanomaterials (NMs) that require testing and the constraints associated with animal models, the majority of studies to elucidate nanotoxicological effects have occurred in vitro, with limited correlation and applicability to in vivo systems and realistic, occupational exposure scenarios.

In this study, we developed and implemented a chronic in vitromodel coupled with lower, regulatory dosages in order to provide a more realistic assessment of NM-dependent consequences and illuminate the implications of long-term NM exposure. When keratinocytes were exposed to 50 nm silver nanoparticles (Ag-NPs), we determined that chronically dosed cells operated …

Remote Beehive Monitoring Using Acoustic Signals, Amro Qandour, Iftekhar Ahmad, Daryoush Habibi, Mark Leppard

Research outputs 2014 to 2021

Recent developments in Wireless Sensor Networks (WSNs) have led to their use in remote data acquisition and automatic data analysis applications, which have proven to be an invaluable tool in a diverse range of fields including biosecurity. Further indications have been found that honeybee health can be monitored and determined through the use of acoustic analysis. In this paper, we present a system that has the ability to remotely detect the presence of pest infestation on a colony of honeybees by comparing the acoustic fingerprint of a hive to a fingerprint of known status. This will aid the goals of …

Numerical Modeling Of Infrared Thermography Techniques Via Ansys, Hayder Abdulnabi Thajeel

Masters Theses

"Several inspection techniques have been developed over years. Recently, infrared thermography (IRT) technology has become a widely accepted as a nondestructive inspection (NDI) technique for different fields and various applications as well. Infrared thermography stands as one of the most an attractive and a successful NDI technique that has ability to detect the object's surface/subsurface defects remotely based on observing and measuring the surface's emitted infrared heat radiation by using an infrared camera. The finite element modeling FEM ANSYS was successfully used for the modelling of several IRT techniques; such as Pulsed Thermography (PT) and Lock-in Thermography (LT) that can …

The Marine Electronic Highway And Routeing Scheme Along The West Coast Of India And Sri Lanka : Feasibility Study : "Aiming Towards A Sustainable Maritime Transportation System", Sellakkannu I. Abul Kalam Azad

World Maritime University Dissertations

No abstract provided.

Cost And Benefit Analysis Of Shore Side Electricity In The Port Of Tanjung Perak, Indonesia, Oscar Yogi Yustiano

World Maritime University Dissertations

No abstract provided.

Systems Metabolic Engineering Of Microbial Cell Factories For The Synthesis Of Value-Added Chemicals, Arul Mozhy Varman

All Theses and Dissertations (ETDs)

Microbial cell factories offer us an excellent opportunity for the conversion of many different cheaply available raw materials into valuable chemicals. Systems metabolic engineering aims at developing rational strategies for the engineering of microbial hosts by providing global level information of a cell. This dissertation focuses on metabolic engineering, bioprocess modeling and pathway analysis, to develop robust microbial cell factories for the synthesis of value-added chemicals. The following research tasks were completed in this regard.

First, statistical models were developed for the prediction of product yields in engineered microbial cell factories - Saccharomyces cerevisiae and Escherichia coli: Chapter 2). A …

3d Conductive Nanocomposite Scaffold For Bone Tissue Engineering, Aref Shahini, Mostafa Yazdimamaghani, Kenneth J. Walker, Margaret A. Eastman, Hamed Hatami-Marbini, Brenda J. Smith, John L. Ricci, Sundar V. Madihally, Daryoosh Vashaee, Lobat Tayebi

Chemical and Biochemical Engineering Faculty Research & Creative Works

Bone healing can be significantly expedited by applying electrical stimuli in the injured region. Therefore, a three-dimensional (3D) ceramic conductive tissue engineering scaffold for large bone defects that can locally deliver the electrical stimuli is highly desired. In the present study, 3D conductive scaffolds were prepared by employing a biocompatible conductive polymer, ie, poly(3,4-ethylenedioxythiophene) poly (4-styrene sulfonate) (PEDOT:PSS), in the optimized nanocomposite of gelatin and bioactive glass. For in vitro analysis, adult human mesenchymal stem cells were seeded in the scaffolds. Material characterizations using hydrogen-1 nuclear magnetic resonance, in vitro degradation, as well as thermal and mechanical analysis showed that …

Microfluidic Generated Egf-Gradients Induce Chemokinesis Of Transplantable Retinal Progenitor Cells Via The Jak/Stat And Pi3kinase Signaling Pathways., Uchenna J. Unachukwu, Moira Sauane, Maribel Vazquez, Stephen Redenti

Publications and Research

A growing number of studies are evaluating retinal progenitor cell (RPC) transplantation as an approach to repair retinal degeneration and restore visual function. To advance cell-replacement strategies for a practical retinal therapy, it is important to define the molecular and biochemical mechanisms guiding RPC motility. We have analyzed RPC expression of the epidermal growth factor receptor (EGFR) and evaluated whether exposure to epidermal growth factor (EGF) can coordinate motogenic activity in vitro. Using Boyden chamber analysis as an initial high-throughput screen, we determined that RPC motility was optimally stimulated by EGF concentrations in the range of 20-400 ng/ml, with decreased …

Promoting Electrocatalytic Activity Of A Composite Sofc Cathode La0.8Sr0.2Mno3+Δ/Ce0.8Gd0.2O2-Δ With Molten Carbonates, Yunhui Gong, Xue Li, Lingling Zhang, Whitney Tharp, Changyong Qin, Kevin Huang

Faculty Publications

The effect of molten carbonates (MCs) on polarization resistance (R_P), a direct measure of oxygen reduction reaction (ORR) activity, of a composite La_0.8Sr_0.2MnO_3+δ/Ce_0.8Gd_0.2O_2-δ (LSM/GDC) solid oxide fuel cell (SOFC) cathode has been systematically investigated in this study over a temperature range of 550–650°C and partial pressure of oxygen (pO₂) span of 10⁻³ ∼ 1 atm. It is shown that the LSM/GDC cathode, either in the pristine or MC-modified states, can be generally modeled by two consecutive parallel circuits consisting of a resistance and a …

Temperature-Dependent Dielectric And Thermal Properties Of Whey Protein Gel And Mashed Potato, Jiajia Chen, Krishnamoorthy Pitchai, Sohan Birla, Ricardo Gonzalez, David Jones, Jeyamkondan Subbiah

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Temperature-dependent dielectric properties (dielectric constant and dielectric loss factor) and thermal properties (thermal conductivity and specific heat capacity) of whey protein gel and mashed potato were measured from -20°C to 100°C. A dielectric properties measurement system and a multipoint temperature calibration protocol were developed. The system consists of an impedance analyzer, a high-temperature coaxial cable, a high-temperature coaxial probe, a micro-climatic chamber and a metal sample holder. Calibrations at two temperatures (25°C and 85°C) were sufficient to accurately measure the dielectric properties of foods from frozen to hot temperatures. Both dielectric constant and dielectric loss factor rapidly increased from -20°C …

Mobile Sensor Data Mining, Jeff Lockhart Fcrh '13

The Fordham Undergraduate Research Journal

At an ever increasing rate, the smartphones and other devices people carry with them in their everyday lives are packed with sensors and processing power. This provides an unprecedented opportunity to apply data mining techniques to people’s activities as they go about their daily lives, without changing their routine. The goal of the Wireless Sensor Data Mining (WISDM) Project is to explore the possibilities of data mining on these powerful mobile platforms.1 Data mining involves extracting knowledge from data using computer algorithms. A major sensor in these devices is the tri-axial accelerometer originally included for screen rotation and advanced gaming. …

2013 Fall Engr333 Project Final Report, 2013 Fall Engr333

ENGR 333

During the fall of 2013, the Engineering 333 class was asked the question, “what would it take for Calvin College to operate a biofuel vehicle from campus resources?” The three primary considerations included:

• Determining the optimal biofuel feedstock in terms of availability, transportation, and processing.
• Selecting a biofuel vehicle for conversion or purchase to operate on the selected feedstock.
• Designing the infrastructure and process required for operating the selected biofuel vehicle. The design and research described below proposes an environmentally sustainable and cost-effective method for integrating a biofuel vehicle into Calvin’s existing infrastructure.

Liquid Core Waveguide Sensors With Single And Multi-Spot Excitation, Lynnell Uilani Wai Yee Zempoaltecatl

Theses and Dissertations

Using silicon based microfabrication and materials, a photonic platform, capable of single bioparticle analysis, has been developed. This platform combines liquid and hollow core waveguides on the micron-scale (5 µm x 12 µm) to isolate femtoliter sized sample volumes. Fluorescence excitation and signals in the visible range are directed into and out of the sample volume at an orthogonal angle to maximize signal-to-noise. In order to guide light in a low-index material antiresonant reflecting optical waveguides (ARROWs) were incorporated into the platform. This thesis reveals the development path of these structures over several device generations including innovations in material, geometries, …

Determining The Internal Structure Of Cement-Based Materials By Using Mri Techniques, Hsiang-Wei Chiang, Ran Huang, Chia-Jung Tsai

Journal of Marine Science and Technology–Taiwan

The primary concerns of the internal structure of cementbased materials involve the distribution of pores, pore size, and the manner in which they are connected. Thoroughly understanding the distributive rules of pores and the approaches to breaking their links can contribute considerably to enhancing the durability of cement-based materials. Several methods are used to measure the pores of cement-based materials; however, they cannot determine the exact location of pores or structural links. Magnetic resonance imaging (MRI) has been widely applied in physical examinations and has matured to the level of being able to obtain 3D images of the organs of …

Interactions Of Peptide Triazole Thiols With Env Gp120 Induce Irreversible Breakdown And Inactivation Of Hiv-1 Virions, Arangassery Bastian, Mark Contarino, Lauren D. Bailey, Rachna Aneja, Diogo Rodrigo Magalhaes Moreira, Kevin Freedman, Karyn Mcfadden, Caitlin Duffy, Ali Emileh

Dartmouth Scholarship

Background: We examined the underlying mechanism of action of the peptide triazole thiol, KR13 that has been shown previously to specifically bind gp120, block cell receptor site interactions and potently inhibit HIV-1 infectivity.

Results: KR13, the sulfhydryl blocked KR13b and its parent non-sulfhydryl peptide triazole, HNG156, induced gp120 shedding but only KR13 induced p24 capsid protein release. The resulting virion post virolysis had an altered morphology, contained no gp120, but retained gp41 that bound to neutralizing gp41 antibodies. Remarkably, HIV-1 p24 release by KR13 was inhibited by enfuvirtide, which blocks formation of the gp41 6-helix bundle during membrane fusion, while …

Computational And Experimental Study Of Degeneration, Damage And Failure In Biological Soft Tissues, Gregory Allen Von Forell

Theses and Dissertations

The purpose of this work was to analyze the biomechanics of degeneration, damage, and failure in biological soft tissues both experimentally and computationally to provide insight into tendon or ligament tearing, tendo-achilles lengthening and lumbar spine dysfunction. For soft tissue tearing, experimental studies for calculating fracture toughness were performed and determined that tendons and ligaments are able to completely resist tear propagation. For tendo-achilles lengthening, a damage model was developed to mimic the behavior of the lengthening that occurs as a result of the percutaneous triple hemisection technique. The model provided insight for predicting the amount of lengthening that occurs …

Molecular Modeling Of Dna For A Mechanistic Understanding Of Hybridization, Terry Jacob Schmitt

Theses and Dissertations

DNA microarrays are a potentially disruptive technology in the medical field, but their use in such settings is limited by poor reliability. Microarrays work on the principle of hybridization and can only be as reliable as this process is robust, yet little is known at the molecular level about how the surface affects the hybridization process. This work uses advanced molecular simulation techniques and an experimentally-parameterized coarse-grain model to determine the mechanism by which hybridization occurs on surfaces and to identify key factors that influence the accuracy of DNA microarrays. Comparing behavior in the bulk and on the surface showed, …

Stability Of Silk And Collagen Protein Materials In Space, Xiao Hu, Waseem K. Raja, Bo An, Olena Tokareva, Peggy Cebe, David L. Kaplan

College of Science & Mathematics Departmental Research

Collagen and silk materials, in neat forms and as silica composites, were flown for 18 months on the International Space Station [Materials International Space Station Experiment (MISSE)-6] to assess the impact of space radiation on structure and function. As natural biomaterials, the impact of the space environment on films of these proteins was investigated to understand fundamental changes in structure and function related to the future utility in materials and medicine in space environments. About 15% of the film surfaces were etched by heavy ionizing particles such as atomic oxygen, the major component of the low-Earth orbit space environment. Unexpectedly, …

Mechanical Properties Of Hydrated Acoustically Sensitive Alginate-Based Microcapsules Confined In A Microfluidic Device As A Function Of Size And Composition, Maryam Mobed-Miremadi, Mallika Keralapura, Tiffany Fong, Rogel Camba

Bioengineering

Understanding the mechanical properties of alginate-based microcapsules according to size and chemical composition allows researchers to zero in on the treatment and methods required to engineer optimized implantable alginate-based artificial cells for chemotherapy. Cross-linked medium viscosity alginate capsules ranging from 1.1% (w/v)-1.8% (w/v) in composition and 200 μm-1200 μm in size, encapsulating ultrasound contrast agents and blue dextran were compressed within a 40 μm high polydimethylsiloxane microfluidic device and subsequently examined using 2D microscopy for strain deformation aimed at the calculation of poisson ratios and volume loss postcompression. Results indicate a decrease in Poisson ratio as a function of alginate …

Situational Awareness/Triage Tool For Use In A Chemical, Biological, Radiological Nuclear Explosive (Cbrne) Environment, John N. Scarlett, Heather L. Gallup, David A. Smith

AFIT Patents

A method of managing patient care and emergency response following a Chemical, Biological, Radiological, or Nuclear Explosive (CBRNE) attack and maintaining compliance with the Health Insurance Portability and Accountability Act (HIPAA). The method including identifying each patient with a unique patient identifier, the identifier based upon the geospatial location of the patient, the geospatial location including at least the latitude and longitude of the patient when first treated, the unique patient identifier being part of patient data. Providing a collection point of patient data to form a patient data database where in the patient location data may be used to …

Methods And Metrics For Human Interaction With Bio-Inspired Robot Swarms, Sean C. Kerman

Theses and Dissertations

In this thesis we propose methods and metrics for human interaction with bio-inspired robot teams. We refine the concept of a stakeholder and demonstrate how a human can use stakeholders to lead a swarm as well as switch the swarm between different collective behaviors. We extend the human interaction metrics of interaction time and interaction effort presented in [1] to swarm systems and introduce the concept of interaction effort. These metrics allow us to understand how well the system performs under human influence. We employ systems theory to estimate these metrics, which is useful because this can be done without …

Fabrication And In Vivo Thrombogenecity Testing Of Nitric Oxide Generating Artificial Lungs, Kagya Amoako, Patrick Montoya, Terry C. Major, Ahmed B. Suhaib, Hitesh Handa, David O. Brant, Mark E. Meyerhoff, Robert H. Bartlett, Keith E. Cook

Mechanical and Industrial Engineering Faculty Publications

Hollow fiber artificial lungs are increasingly being used for long-term applications. However, clot formation limits their use to 1–2 weeks. This study investigated the effect of nitric oxide generating (NOgen) hollow fibers on artificial lung thrombogenicity. Silicone hollow fibers were fabricated to incorporate 50 nm copper particles as a catalyst for NO generation from the blood. Fibers with and without (control) these particles were incorporated into artificial lungs with a 0.1 m2 surface area and inserted in circuits coated tip-to-tip with the NOgen material. Circuits (N = 5/each) were attached to rabbits in a pumpless, arterio-venous configuration and run for …

Layered Long Term Co-Culture Of Hepatocytes And Endothelial Cells On A Transwell Membrane: Toward Engineering The Liver Sinusoid, Young Bok (Abraham) Kang, Siddhartha Rawat, Joseph Cirillo, Michael Bouchard, Hongseok (Moses) Noh

Faculty Publications - Biomedical, Mechanical, and Civil Engineering

This paper presents a novel liver model that mimics the liver sinusoid where most liver activities occur. A key aspect of our current liver model is a layered co-culture of primary rat hepatocytes (PRHs) and primary rat liver sinusoidal endothelial cells (LSECs) or bovine aortic endothelial cells (BAECs) on a transwell membrane. When a layered co-culture was attempted with a thin matrigel layer placed between hepatocytes and endothelial cells to mimic the Space of Disse, the cells did not form completely separated monolayers. However, when hepatocytes and endothelial cells were cultured on the opposite sides of a transwell membrane, PRHs …

Immobilization Of Lead In Contaminated Firing Range Soil Using Biochar, Deok Hyun Moon, Jae-Woo Park, Yoon-Young Chang, Yong Sik Ok, Sang Soo Lee, Mahtab Ahmad, Agamemnon Koutsospyros, Jeong Hun Park, Kitae Baek

Civil Engineering Faculty Publications

Soybean stover-derived biochar was used to immobilize lead (Pb) in military firing range soil at a mass application rate of 0 to 20 wt.% and a curing period of 7 days. The toxicity characteristic leaching procedure (TCLP) was performed to evaluate the effectiveness of the treatment. The mechanism responsible for Pb immobilization in military firing range soil was evaluated by scanning electron microscopy-energy dispersive x-ray spectroscopy (SEM-EDX) and x-ray absorption fine structure (XAFS) spectroscopy analyses. The treatment results showed that TCLP Pb leachability decreased with increasing biochar content. A reduction of over 90 % in Pb leachability was achieved upon …

Ole Miss Engineer 2013-2014

Ole Miss Engineer

No abstract provided.