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A Three Constituent Mixture Theory Model Of Cutaneous And Subcutaneous Tissue In The Context Of Neonatal Pressure Ulcer Etiology And Prevention, Anne Dye Zakrajsek

Open Access Dissertations

Localized ischemia, impaired interstitial fluid flow, and sustained mechanical loading of cells have all been hypothesized as mechanisms of pressure ulcer (PrU) etiology. Time-varying loading has experimentally been shown to increase fluid flow in human skin in vivo. Towards the design of prophylactic protocols and treatment modalities for PrU management there is a need for an analytical model to investigate the local fluid flow characteristics of skin tissue under time-varying loading. In this study, a triphasic mixture theory model with constituents of extracellular matrix, interstitial fluid, and blood was calibrated and validated and used to investigate stress and fluid velocity …

International Water And Food Security Development: Performance Evaluation And Assessment Of Research Needs At Multiple Scales, Caitlin Anne Grady

Open Access Dissertations

Water and food security remain the top development challenges of the decade, and perhaps the century. Since the Millennium Development Goals were established in 2000, billions of people have obtained access to more food, better nutrition, improved water, and basic sanitation facilities worldwide. This progress has been accomplished through the dedication of international organizations, non-governmental organizations, country-level governments, private corporations, and individuals at international, regional, and local scales. Truly tremendous strides have been made in water and food provisioning for humans worldwide. These past two decades have also seen the largest population growth on record, the highest rates of childhood …

Ablation Of Cardiac Tissue With Nanosecond Pulsed Electric Fields: Experiments And Numerical Simulations, Fei Xie

Biomedical Engineering Theses & Dissertations

Cardiac ablation for the treatment of cardiac arrhythmia is usually performed by heating tissue with radio-frequency (RF) electrical currents to create conduction-blocking lesions in order to stop the propagation of electrical waves. Problems associated with RF ablation are recurrence of arrhythmias after successful treatments, tissue loss beyond the targeted tissue, long duration of the ablation procedure, and thermal side effects including thrombus formation that may lead to stroke. Here, we propose a new, non-thermal ablation method using nanosecond pulsed electric fields (nsPEFs) with better-controlled ablation volume, shorter procedure time, and no thermal side effects. We demonstrate that we can create …

Effect Of Malathion On The Microbial Ecology Of Activated Sludge, Seth K. Martin

Theses and Dissertations

Decontamination activities may cause the release of contaminated washwater into the wastewater that eventually flows into a wastewater treatment facility. This raises concerns about the effect of chemical warfare agents (CWAs) on the microbial consortia that are responsible for cleaning the wastewater. This study investigated the impact of malathion on the microbial ecology of laboratory scale activated sludge communities. The Simpson Reciprocal Index decreased for three bioreactors operated in the absence of malathion, which showed that the microbial assemblage became less diverse during the course of the study. The species identified in the bioreactors belonged to well-known groups of heterotrophic …

A Ten-Year Search For Synchronous Cells: Obstacles, Solutions, And Practical Applications, Charles E. Helmstetter

Biomedical Engineering and Sciences Faculty Publications

My effort to use synchronously dividing cultures to examine the Escherichia coli cell cycle involved a 10-year struggle with failure after failure punctuated by a few gratifying successes, especially at the end. In this essay, I recount my personal journey in this obsessive experimental pursuit. That narrative is followed by a description of a simplified version of the "baby machine," a technique that was developed to obtain minimally disturbed, synchronously growing E. coli cells. Subsequent studies with this methodology led to an understanding of the basic properties of the relationship between chromosome replication and cell division. Accordingly, I end this …

National Livestock Policy Of Nepal: Needs And Opportunities, Upendra B. Pradhanang, Soni M. Pradhanang, Arhan Sthapit, Nir Y. Krakauer, Ajay K. Jha, Tarendra Lakhankar

Publications and Research

This paper describes Nepal’s national livestock policies and considers how they can be improved to help meet the pressing national challenges of economic development, equity, poverty alleviation, gender mainstreaming, inclusion of marginalized and underprivileged communities, and climate vulnerability. Nepal is in the process of transforming its government from a unitary system to a federal democratic structure through the new constitution expected by 2015, offering the opportunity to bring a new set of priorities and stakeholders to policymaking. Nepal’s livestock subsector comes most directly within the purview of the National Agricultural Policy 2004, Agro-Business Policy, 2006 and Agricultural Sectoral Operating Policies …

P-08 Paper-Based Biosensor For Colorimetric Detection Of Psa Biomarker, Alyson E. Drew

Honors Scholars & Undergraduate Research Poster Symposium Programs

Prostate cancer is detected by excess PSA in the bloodstream, a protein the prostate overproduces when tumors are present. In this paper, we designed an origami paper analytical device, specific to PSA using gold nanoparticles which provide a colorimetric, qualitative, inexpensive, disposable design to detect PSA. Our device separates the sample laterally to prevent back-mixing and vertically to enable addition of novel particles. The design was optimized for channel thickness, size, proper heating, type of paper involved, and amount of sample solution needed. We are currently concept-testing of PSA qualitative detection via sandwich assay to target detection levels < 100ng/mL.

Compensatory Enlargement Of Ossabaw Miniature Swine Coronary Arteries In Diffuse Atherosclerosis, Jenny Susana Choy, Tong Luo, Yunlong Huo, Thomas Wischgoll, Kyle Schultz, Shawn D. Teague, Michael Sturek, Ghassan S. Kassab

Computer Science and Engineering Faculty Publications

Studies in human and non-human primates have confirmed the compensatory enlargement or positive remodeling (Glagov phenomenon) of coronary vessels in the presence of focal stenosis. To our knowledge, this is the first study to document arterial enlargement in a metabolic syndrome animal model with diffuse coronary artery disease (DCAD) in the absence of severe focal stenosis. Two different groups of Ossabaw miniature pigs were fed a high fat atherogenic diet for 4 months (Group I) and 12 months (Group II), respectively. Group I (6 pigs) underwent contrast enhanced computed tomographic angiography (CCTA) and intravascular ultrasound (IVUS) at baseline and after …

Advancing Biomechanical Research Through A Camelid Model Of The Human Lumbar Spine, Dean K. Stolworthy

Theses and Dissertations

The increasing incidence of disc degeneration and its correlation with lower back pain is an alarming trend in modern society. The research of intervertebral disc degeneration and low back pain would greatly benefit from additional methods to study its etiology and possible treatment methods. A large animal model that maintains the biological and mechanical environment that is most similar to the human lumbar spine could provide substantial improvements in understanding and resolving the problem of intervertebral disc related low back pain.This dissertation presents my doctoral work of investigating the potential for the camelid cervical spine to serve as a suitable …

Superheated Liquid And Supercritical Denatured Ethanol Extraction Of Antioxidants From Crimson Red Grape Stems, Johnathon Wenzel, Lihua Wang, Cheryl Samaniego, La'shyla Nelson, Korrine Ketchum, Michelle Ammerman, Ali Zand

Chemical Engineering Publications

Grapes are widely known for health benefits due to their antioxidant content. In wine production, grape stems are often discarded, though they has a higher content of antioxidants than the juice. The effectiveness of using an environmentally friendly solvent, ethanol, as a superheated liquid and supercritical fluid to extract antioxidant compounds from grape stems of organically grown Crimson Seedless grapes was evaluated. The Ferric Reducing Ability of Plasma (FRAP) assay and the Total Phenolic Content (TPC), or Folin-Ciocalteu assay, were used to quantify the antioxidant power of grape stem extracts. The extractions were performed at temperatures between 160°C and 300°C …

Concomitant Uptake Of Antimicrobials And Salmonella In Soil And Into Lettuce Following Wastewater Irrigation, J. Brett Sallach, Yuping Zhang, Laurie Hodges, Daniel D. Snow, Xu Li, Shannon L. Bartelt-Hunt

Department of Civil and Environmental Engineering: Faculty Publications

The use of wastewater for irrigation may introduce antimicrobials and human pathogens into the food supply through vegetative uptake. The objective of this study was to investigate the uptake of three antimicrobials and Salmonella in two lettuce cultivars. After repeated subirrigation with synthetic wastewater, lettuce leaves and soil were collected at three sequential harvests. The internalization frequency of Salmonella in lettuce was low. A soil horizon-influenced Salmonella concentration gradient was determined with concentrations in bottom soil 2 log CFU/g higher than in top soil. Lincomycin and sulfamethoxazole were recovered from lettuce leaves at concentrations as high as 822 ng/g and …

An Affordance-Based Methodology For Package Design, Javier De La Fuente Phd, Stephanie Gustafson, Colleen L. Twomey, Laura Bix

Industrial Technology and Packaging

The term affordance describes an object's utilitarian function or actionable possibilities. Product designers have taken great interest in the concept of affordances because of the bridge they provide relating to design, the interpretation of design and, ultimately, functionality in the hands of consumers. These concepts have been widely studied and applied in the field of psychology but have had limited formal application to packaging design and evaluation. We believe that the concepts related to affordances will reveal novel opportunities for packaging innovation. To catalyse this, presented work had the following objectives: (a) to propose a method by which packaging designers …

An Electrospinning Apparatus For The Production Of Heterogeneous Scaffolds And Their Application In Cartilage Regeneration, Andrew Ryan Hollingsworth

Theses

The human body contains a vast array of soft and hard tissues, each with their own unique set of physical, chemical, mechanical, and electrical properties. It is the combination of these tissues that allows the human body to function as it does. However, these tissues are subject to wear, fatigue, and injury and thus can potentially limit the functions of the body. Therefore, tissue engineering has sought various natural and synthetic materials that can be used for fabrication of cellular scaffolds that have the ability to promote cellular attachment, proliferation, differentiation, and eventual whole tissue formation for replacement and repair …

Generation Of Multicellular Tumor Spheroids With Microwell-Based Agarose Scaffolds For Drug Testing, Xue Gong, Chao Lin, Jian Cheng, Jiansheng Su, Hang Zhao, Tianlin Liu, Xuejun Wen, Peng Zhao

Chemical and Life Science Engineering Publications

Three dimensional multicellular aggregate, also referred to as cell spheroid or microtissue, is an indispensable tool for in vitro evaluating antitumor activity and drug efficacy. Compared with classical cellular monolayer, multicellular tumor spheroid (MCTS) offers a more rational platform to predict in vivo drug efficacy and toxicity. Nevertheless, traditional processing methods such as plastic dish culture with nonadhesive surfaces are regularly time-consuming, laborious and difficult to provide uniform-sized spheroids, thus causing poor reproducibility of experimental data and impeding high-throughput drug screening. In order to provide a robust and effective platform for in vitro drug evaluation, we present an agarose scaffold …

Nephrotoxic Contaminants In Drinking Water And Urine, And Chronic Kidney Disease In Rural Sri Lanka, Tewodros Rango, Marc Jeuland, Herath Manthrithilake, Peter G. Mccornick

Daugherty Water for Food Global Institute: Faculty Publications

Chronic kidney disease of unknown (“u”) cause (CKDu) is a growing public health concern in Sri Lanka. Prior research has hypothesized a link with drinking water quality, but rigorous studies are lacking. This study assesses the relationship between nephrotoxic elements (namely arsenic (As), cadmium (Cd), lead (Pb), and uranium (U)) in drinking water, and urine samples collected from individuals with and/or without CKDu in endemic areas, and from individuals without CKDu in nonendemic areas. All water samples—from a variety of source types (i.e., shallow and deep wells, springs, piped, and surface water)—contained extremely low concentrations of nephrotoxic elements, and all …

Assessment Of Village Chicken Production Systems In Kambata Tambaro And Wolaita Zones, Snnpr, Ethiopia, Aman Getiso, Fitsum Tessema, Mesfin Mekonnen, Addisu Jimma, Bereket Zeleke

Daugherty Water for Food Global Institute: Faculty Publications

The study was conducted in four woredas (Damot Gale,Of a, Angacha and Hadero Tunto) the first two of them found in Wolaita zone and two of them in Kambata Tambaro Zone of SNNPR, Ethiopia respectively. A cross-sectional survey was conducted in the study areas to assess village chicken production systems, productive and reproductive performance of village chicken and identifying constraints to village chicken production. Stratified random sampling technique was used to select 240 farming households and administer a pre-tested and structured questionnaire. The results showed that the mean age of interviewed farmers was 37.8±9.3 years; average family size & chicken …

03. Biology, Northeastern State University

Oklahoma Research Day Abstracts

No abstract provided.

Generation Of Multicellular Tumor Spheroids With Microwell-Based Agarose Scaffolds For Drug Testing, Xue Gong, Chao Lin, Jian Cheng, Jiansheng Su, Hang Zhao, Tianlin Liu, Xuejun Wen, Peng Zhao

Chemical and Life Science Engineering Publications

Three dimensional multicellular aggregate, also referred to as cell spheroid or microtissue, is an indispensable tool for in vitro evaluating antitumor activity and drug efficacy. Compared with classical cellular monolayer, multicellular tumor spheroid (MCTS) offers a more rational platform to predict in vivo drug efficacy and toxicity. Nevertheless, traditional processing methods such as plastic dish culture with nonadhesive surfaces are regularly time-consuming, laborious and difficult to provide uniform-sized spheroids, thus causing poor reproducibility of experimental data and impeding high-throughput drug screening. In order to provide a robust and effective platform for in vitro drug evaluation, we present an agarose scaffold …

Effect Of Four Approaches To Oral Feeding Progression On Clinical Outcomes In Preterm Infants, Rita H. Pickler, Barbara A. Reyna, Paul A. Wetzel, Mary Lewis

Biomedical Engineering Publications

Background. The purpose of this study of preterm infants was to test the effect of four approaches to the time of transition from gavage to full oral feedings, time to discharge, and weight gain during the transition.Methods. A randomized experimental design was used with four intervention groups: early start (32 weeks' postmenstrual age)/slow progressing experience (gradually increasing oral feedings offered per day); early start/maximum experience (oral feedings offered at every feeding opportunity); late start (34 weeks' postmenstrual age)/slow progressing experience; and late start/maximum experience. Results. The analysis included 86 preterm infants. Once oral feedings were initiated, …

Research Week 2015, Linda Gardiner, David Owerbach

Office of Research Institutional Research and Scholarship

No abstract provided.

2015 Scholarly Productivity Report, Missouri University Of Science And Technology

Civil, Architectural and Environmental Engineering Scholarly Productivity Reports

No abstract provided.

Neuroimaging And Neuromodulation Approaches To Study Eating Behavior And Prevent And Treat Eating Disorders And Obesity, D. Val-Laillet, E. Aarts, B. Weber, M. Ferrari, V. Quaresima, L. E. Stoeckel, M. Alonso-Alonso, M. Audette, C. H. Malbert, E. Stice

Computational Modeling & Simulation Engineering Faculty Publications

Functional, molecular and genetic neuroimaging has highlighted the existence of brain anomalies and neural vulnerability factors related to obesity and eating disorders such as binge eating or anorexia nervosa. In particular, decreased basal metabolism in the prefrontal cortex and striatum as well as dopaminergic alterations have been described in obese subjects, in parallel with increased activation of reward brain areas in response to palatable food cues. Elevated reward region responsivity may trigger food craving and predict future weight gain. This opens the way to prevention studies using functional and molecular neuroimaging to perform early diagnostics and to phenotype subjects at …

09. Environmental Science, Northeastern State University

Oklahoma Research Day Abstracts

No abstract provided.

Evaluating The Potential For Passive Greywater Irrigation In Northern Ghana, Chelsea L. Fagan

Dissertations, Master's Theses and Master's Reports - Open

Water scarcity, malaria, and malnutrition are all concerns facing the people of Chirifoyili and the Northern Region of Ghana. Greywater pooling outside of homes increases human exposure to pathogens and provides breeding grounds for disease carrying insects, especially malaria spreading mosquitoes. This project looks at draining this water away from the home and using it to irrigate vegetables, fruit trees and other beneficial plants.

The purpose of this study is to estimate the effects of greywater irrigation on the growth of plants commonly found in village home gardens. The field project consisted of constructing and managing household greywater irrigation systems …

Layered, Flexible Drug Delivery Films For The Prevention Of Fibrotic Scar Tissue Formation, Cheryl L. Rabek

Theses and Dissertations--Biomedical Engineering

Open wounds account for about 50% of military injuries and 10% of non‐fatal traffic injuries. Scar tissue formation in these wounds may be reduced or prevented if treated with a combination of molecules whose release is tuned to the healing phases. The goal of this research was to develop flexible, layered drug delivery films for sequential, localized release of anti‐inflammatory, anti‐oxidant, and anti‐fibrotic molecules to soft tissue.

Films were composed of cellulose acetate phthalate (CAP) and Pluronic F‐127 (Pluronic). To impart flexibility, plasticizers, triethyl citrate (TEC) or tributyl citrate (TBC), were added. Mechanical analysis was performed on films as prepared …

Accounting For The Short Term Substitution Effects Of Walking And Cycling In Sustainable Transportation, Daniel P. Piatkowski, Kevin J. Krizek, Susan L. Handy

Community and Regional Planning Program: Faculty Scholarly and Creative Activity

The environmental benefits of bicycling and walking depend on the degree to which their use substitutes for car driving. Assuming that every walking and bicycling trip replaces a driving trip is likely to produce overestimates of the potential for such modes to reduce vehicle travel and city-scale greenhouse gas emissions. Measuring this ‘‘substitution effect’’ is not straightforward. There are many dimensions of the substitution effect, including trip type, substituting mode, extent, time horizon, and activity patterns. Previously used approaches to measure substitution include indirect inference and direct questioning. This study piloted an intercept survey using the direct questioning approach at …

Body Mass Bias Mitigation For Females In Military Physical Readiness Testing Through Load Carriage Implementation, Aaron Yeaton

Electronic Theses and Dissertations

The US Military requires specific fitness testing, known as the Physical Readiness Test (PRT), for its members to determine their overall fitness levels. The test currently being used has been shown to have bias towards heavier mass individuals of up to 20%. Prior research has been completed and several recommendations have been made to eliminate mass bias, but this has been conducted almost exclusively on males. There is very little data and research on military physical fitness testing for women besides combat specific evolution exercises. A possibility exists to eliminate the bias for females through implementing load carriage during PRT …

Quantification Of Food Waste Disposal In The United States: A Meta-Analysis, Krista L. Thyberg, David J. Tonjes, Jessica Gurevitch

Technology & Society Faculty Publications

Food waste has major consequences for social, nutritional, economic, and environmental issues, and yet the amount of food waste disposed in the U.S. has not been accurately quantified. We introduce the transparent and repeatable methods of meta-analysis and systematic reviewing to determine how much food is discarded in the U.S., and to determine if specific factors drive increased disposal. The aggregate proportion of food waste in U.S. municipal solid waste from 1995 to 2013 was found to be 0.147 (95% CI 0.137-0.157) of total waste, which is lower than that estimated by USEPA for the same period (0.176). The proportion …

Sustainable Disposal Of Coal Processing Waste Streams, Mohammad Rezaee

Theses and Dissertations--Mining Engineering

Modern coal preparation facilities incorporate a wide array of solid-solid and solid-liquid separation processes for rejecting mineral matter to meet market specifications. The coarse mineral matter is typically placed into engineered refuse piles whereas the fine refuse is either stored in impoundments or co-disposed with the coarse refuse. The discharge water from the refuse material represents an environmental concern due to the potential release of trace elements, and the subsequent elevation of total dissolved solids and conductivity. The research findings reported in this dissertation addresses sustainable coal processing waste disposal through using strategies aimed at minimizing the environmental impacts. To …

Patient Populations, Clinical Associations, And System Efficiency In Healthcare Delivery System, Yazhuo Liu

USF Tampa Graduate Theses and Dissertations

The efforts to improve health care delivery usually involve studies and analysis of patient populations and healthcare systems. In this dissertation, I present the research conducted in the following areas: identifying patient groups, improving treatments for specific conditions by using statistical as well as data mining techniques, and developing new operation research models to increase system efficiency from the health institutes’ perspective. The results provide better understanding of high risk patient groups, more accuracy in detecting disease’ correlations and practical scheduling tools that consider uncertain operation durations and real-life constraints.