Digital Commons Network^™

Analyzing Spinach Growth Using Vertical Garden Techniques And Local Water Sources For Urban Gardens, Julia Ashlyn Manzella

All ETDs from UAB

The University of Alabama at Birmingham undergraduate and graduate programs’ continued involvement in the Birmingham-Jefferson County community have identified food insecure areas, known as food deserts, and associated health issues negatively impacting the community due to poor diets and lack of access to healthy foods. Farm stands and community gardens around the Birmingham-metropolitan area have increased healthy food availability during the past several years to encourage healthier eating habits. To combat food insecurity, sustainable alternative growing techniques for urban agriculture need to be implemented and incorporated into local community gardens. Alternative gardening techniques such as vertical gardens maximize yield in …

Impact Of Cryptococcus Neoformans Polysaccharide Capsule On Astrocytes, Yeon Hwa Woo

Open Access Theses & Dissertations

Cryptococcus neoformans is a neurotropic, opportunistic, encapsulated, yeast-like fungus that causes life-threatening meningoencephalitis in both healthy and immunocompromised individuals. C. neoformans infects 1,000,000 people and causes 210,000 deaths worldwide annually, mostly in sub-Saharan Africa. The polysaccharide capsule of C. neoformans is the fungus major virulent factor. Glucuronoxylomannan (GXM), the main component of the capsule is abundantly released into brain tissue during C. neoformans infection, causing immunosuppressive defects in the host. C. neoformans is inhaled from the environment, disseminate via bloodstream, and crosses the blood brain barrier (BBB) to cause cryptococcal meningoencephalitis (CM). Although CM causes significant mortality in HIV/AIDS patients, …

Supercritical Processed Decellularized Extracellualr Matrix As Regeneration Therapeutics Applying 3d Printing, Seungwon Chung

Open Access Theses & Dissertations

Extracellular matrix has been broadly applied and show great promise in medical applications and tissue engineering. Product of extracellular matrix (ECM) should be treated with decellularization and purification process. Supercritical carbon dioxide treatment is of particular interest for purifying ECM because of its medically available and rapid speed of process. However, it is not fully researched in treatment of biomaterials for tissue engineering. Therefore, we investigated optimal conditions of supercritical carbon dioxide processing in different extracting parameters from porcine adipose tissue. The 3-day, dual treatment including enzymatic decellularization and supercritical fluid extraction of pork adipose tissue were performed. Two types …

The Role Of Geospatial Information And Effective Partnerships In The Implementation Of The International Agenda For Sustainable Development, Etta Delores Jackson

Antioch University Dissertations & Theses

The former United Nations Secretary-General Ban Ki-Moon (2014), repeated the core promise in the 1986 UN Declaration on the Right to Development, in which the General Assembly called for an approach guaranteeing meaningful participation of everyone in development and the fair distribution of the benefits of that development. To this end, partnerships are central and can lead to the dignity of the citizens involved as they participate in the development of their own communities. This dissertation research conducted in Manyatta A and B in the Port City of Kisumu, Kenya sought to do just that. The purpose of this study …

Life Cycle Studies To Evaluate The Physiological And Biochemical Effects Of Copper Oxide And Surface-Coated Titanium Dioxide Nanoparticles On Green Onion (Allium Fistulosum) And Carrot (Daucus Carota L.) Plants: Insights Utilizing Two-Photon Microscopy And Spectroscopy, Yi Wang

Open Access Theses & Dissertations

With the recent increase of nanomaterial production, nano copper oxide (nCuO) and surface-modified titanium dioxide nanoparticles (nTiO2) are among the most widely applied nanoparticles in industry and daily lives. Their use has resulted in accumulation in soils as a consequence of their direct or indirect release. Hence, these NPs may raise a potential risk to crops cultivated in soils. Moreover, the physiological effects of nCuO on green onion (Allium fistulosum) and surface-coated nTiO2 on full-grown carrot (Daucus carota L.) are still unknown. Green onion is characterized by its high content of the antioxidant allicin, and carrot is a worldwide economic …

A Sample Weight And Adaboost Cnn-Based Coarse To Fine Classification Of Fruit And Vegetables At A Supermarket Self-Checkout, Khurram Hameed, Douglas Chai, Alexander Rassau

Research outputs 2014 to 2021

© 2020 by the authors. Licensee MDPI, Basel, Switzerland. The physical features of fruit and vegetables make the task of vision-based classification of fruit and vegetables challenging. The classification of fruit and vegetables at a supermarket self-checkout poses even more challenges due to variable lighting conditions and human factors arising from customer interactions with the system along with the challenges associated with the colour, texture, shape, and size of a fruit or vegetable. Considering this complex application, we have proposed a progressive coarse to fine classification technique to classify fruit and vegetables at supermarket checkouts. The image and weight of …

การเตรียมและศึกษาคุณสมบัติของไบเจลน้ำมันถั่วดาวอินคา, ญาดา ยะก๊บ

Chulalongkorn University Theses and Dissertations (Chula ETD)

ไบเจลเป็นเทคโนโลยีแบบใหม่ที่มีศักยภาพมากในการนำส่งสารออกฤทธิ์สำหรับการประยุกต์ใช้ทางเครื่องสำอาง วัตถุประสงค์ของงานวิจัยนี้เพื่อพัฒนาและกำหนดลักษณะระบบไบเจลจากน้ำมันถั่วดาวอินคาที่บรรจุสารเรตินิลปาล์มมิเตท ไบเจลถูกเตรียมโดยการทำให้เป็นเนื้อเดียวกันด้วยแรงเฉือนสูง ระหว่าง ส่วนออร์กาโนเจลด้วยน้ำมันถั่วดาวอินคา และสารซิลิกาไดเมททิลซิลิเลท ส่วนไฮโดรเจลประกอบด้วยคาร์โบพอล 940 และน้ำ พารามิเตอร์ตัวแปรที่ศึกษา ได้แก่ อัตราเฉือน (1200-1500 rpm), ความเข้มข้นของเจลของไฮโดรเจล (0.3-0.5% โดยน้ำหนัก), ความเข้มข้นของสารก่อเจลในออร์กาโนเจล (6-8% โดยน้ำหนัก) และอัตราส่วนของออร์กาโนเจลต่อไฮโดรเจล (10:90-50:50) ผลที่ได้เสดงถึงสภาวะที่เหมาะสมในการเตรียม ได้แก่ อัตราเฉือน 1500 rpm, ความเข้มข้นของเจลของไฮโดรเจล 0.5% โดยน้ำหนัก, ความเข้มข้นของสารก่อเจลในออร์กาโนเจล (6 หรือ 8% โดยน้ำหนัก) และอัตราส่วนของออร์กาโนเจลต่อไฮโดรเจล ที่ 10:90 โครงสร้างจุลภาค คุณสมบัติทางกายภาพ การเรียงตัวและการวิเคราะห์ขนาดอนุภาคถูกตรวจสอบโดยกล้องจุลทรรศน์ ไบเจลให้การปลดปล่อยสารสารเรตินิลปาล์มมิเตทแบบค่อย ๆ ปลดปล่อยและมีคุณลักษณะที่ดี เป็นเวลา 3 เดือน

การออกแบบและพัฒนาผลิตภัณฑ์ไม้ไอศกรีมจากผงกล้วยดิบ, สิปโปทัย ศรีนิลรัตน์

Chulalongkorn University Theses and Dissertations (Chula ETD)

งานวิจัยนี้ครั้งนี้มีวัตถุประสงค์ เพื่อพัฒนาไม้ไอศกรีมจากผงกล้วยดิบ โดยอาศัยหลักการออกแบบการทดลอง เพื่อเป็นทางเลือกในการเพิ่มมูลค่าของกล้วยให้กับเกษตรกร และเป็นแนวทางให้กับผู้ประกอบกิจการในการลดขยะที่เกิดจากการทิ้งไม้ไอศกรีมแบบเดิม โดยไม้ไอศกรีมที่ผลิตได้ต้องมีคุณภาพที่เหมาะสมกับการใช้งานและได้รับการยอมรับจากผู้บริโภค ซึ่งเริ่มจากทำการศึกษาวิธีการขึ้นรูปผลิตภัณฑ์ผง ด้วยการค้นคว้างานวิจัยและทดลองเบื้องต้น โดยพบว่าแป้งสาลีมีคุณสมบัติของตัวประสานที่ดีที่สุด และวิธีการผลิตอ้างอิงจากกระบวนการผลิตผลิตภัณฑ์ขนมอบในอุตสาหกรรม จากนั้นทำการศึกษาปัจจัย 3 ปัจจัย คือ สัดส่วนผงกล้วยดิบต่อแป้งสาลีเอนกประสงค์ ปริมาณน้ำตาล และเวลาที่ใช้ในการอบ โดยการออกแบบการทดลองแบบบล็อกสุ่มสมบูรณ์ ซึ่งทำการทดสอบคุณภาพทางกายภาพด้านความแข็ง ความเปราะ และคุณภาพทางจุลินทรีย์ของผลิตภัณฑ์ ซึ่งสามารถสรุปเงื่อนไขที่เหมาะสมในการผลิตไม้ไอศกรีมจากผงกล้วยดิบที่ระดับนัยสำคัญ 0.05 ได้ดังนี้ คือ สัดส่วนผงกล้วยดิบต่อแป้งสาลีเอนกประสงค์ตั้งแต่ร้อยละ 50:50 จนถึง 80:20 ปริมาณน้ำตาลร้อยละ 40 โดยน้ำหนักส่วนผสมแห้ง และเวลาที่ใช้ในการอบ 40 นาที ซึ่งมีผลของค่าความแข็งเฉลี่ยใกล้เคียงกับไม้ไอศกรีมจากไม้มากที่สุดอยู่ในช่วง 31.49 ± 2.19 นิวตัน ถึง 38.53 ± 4.86 นิวตัน และผลการทดสอบค่าวอเตอร์แอคทิวิตี้อยู่ในเกณฑ์ที่ไม่ทำให้เกิดการเจริญเติบโตของจุลินทรีย์ในระหว่างการเก็บรักษาอยู่ในช่วง 0.050 ± 0.002 ถึง 0.112 ± 0.062 นอกจากนี้ ไม้ไอศกรีมจากผงกล้วยดิบยังสามารถรับประทานได้ ซึ่งได้ศึกษาการยอมรับของผู้บริโภคที่มีต่อไม้ไอศกรีมจากผงกล้วยดิบ โดยการประเมินคุณภาพทางประสาทสัมผัสจากการทำแบบสอบถาม และผลทดสอบการยอมรับผลิตภัณฑ์ไม้ไอศกรีมจากผงกล้วยดิบในคุณลักษณะด้านต่างๆ ได้แก่ สี กลิ่น รสชาติ เนื้อสัมผัส และความชอบโดยรวมพบว่าอยู่ในระดับปานกลางถึงมาก โดยผลิตภัณฑ์ที่มีเงื่อนไขสัดส่วนผงกล้วยดิบต่อแป้งสาลีเอนกประสงค์ร้อยละ 50:50 ปริมาณน้ำตาลร้อยละ 40 โดยน้ำหนักส่วนผสมแห้ง และ เวลาที่ใช้ในการอบ 40 นาที มีระดับความชอบโดยรวมมากที่สุด และจากการประเมินต้นทุนในการผลิตไม้ไอศกรีมจากกล้วยดิบผงจะมีต้นทุนค่าใช้จ่ายโดยประมาณอยู่ที่ชิ้นละ 1.57 บาท

Picking-And-Placing Food With Vacuum Pad And Soft Gripper For Industrial Robot Arm, Wiwat Tulapornpipat

Chulalongkorn University Theses and Dissertations (Chula ETD)

Human with different conditions such as disease, allergy, gender, ethnicity, or religion need different types of food. Nowadays, people prefer to choose diet based on nutrition value over price such as composition of food or the impact on body, so personalized or customized food could potentially be a big market in few years. With this aspect, the assembly robot becomes a good choice in the food industry to produce the small amount of packed meal with highly adjustable ingredients to match with the requirements. With the automation system, the cooking process is faster, cheaper and safer, yet coming with good …

A Study Of Customers' Willingness To Purchase Organic Fresh Milk In Thailand, Jeerawan Punwaree

Chulalongkorn University Theses and Dissertations (Chula ETD)

Nowadays, most consumers often are care about health due to the COVID-19 and personal needs. Dairy products are specific products that are generally consumed by every age group, from children, teenagers, adults, to the elderly. Organic milk could provide an advantageous alternative for health conscious consumers as it must be certified by government agencies prior to market launching. There are many studies supported that organic milk is more beneficial than conventional milk. Theory of Planned Behavior (TPB) was applied to study the factors affected consumers' willingness to purchase organic fresh milk in Thailand. Through the questionnaire method and analysis by …

Strategies And Devices For Improving Respiratory Drug Delivery To Infants And Children With Cystic Fibrosis, Karl Bass

Theses and Dissertations

Cystic Fibrosis (CF) is a degenerative disease, which causes thickening of the airway surface liquid and reduced mucociliary clearance, which provides an ideal habitat for bacterial infections. Early treatment of CF in children can prevent chronic infection, improve quality of life, and increase life expectancy. The most predominant bacteria found in CF-diseased lungs is Pseudomonas aeruginosa (Pa), which can be treated with inhaled tobramycin. Excipient enhanced growth (EEG) powder formulations are well suited for administering tobramycin to children, as the EEG approach provides minimal upper airway loss and targeted drug delivery. This method uses an initially small aerosol for high …

Reaction Profiling Of Extracellular Protein Phosphorylation Through A Microfluidic Reactor Coupled With Raman Spectroscopy, Abigail Casey

Theses and Dissertations

Diseases and disorders in the human body are considered abnormalities of proper cellular function. Understanding the signal transduction mechanisms that cause these abnormalities is crucial to developing earlier detection methods, better treatment options and effective cures. While current diagnostic procedures are powerful tools in diagnosing diseases, they are ineffective in informing physicians on the real-time behavior of the signal transduction mechanisms associated with diseases and disorders. Currently, disease progression is monitored over time through routine patient visits and testing by one or more of the above techniques. By developing an approach that can monitor structural and conformational changes of proteins …

Cognitive Performance In Midlife Type 2 Diabetes: Results Form The Enbind Study, A. H. Dyer, L. Mckenna, G. Gamage, N. M. Bourke, Isabelle Killane, M. Widdowson, C. P. Woods, J. Gibney, R. Reilly, D. O'Neill, S. P. Kennelly

Articles

Aims: To establish the impact of uncomplicated type 2 diabetes on cognitive and neuropsychological performance in midlife. Methods: We performed a cross-sectional study of middle-aged adults with uncomplicated type 2 diabetes and a cohort of healthy control participants. General cognition was assessed using the Montreal Cognitive Assessment test and neuropsychological assessment was undertaken using a detailed neuropsychological assessment battery. Results: A total of 152 participants (102 with type 2 diabetes and 50 controls) were recruited (mean age 52 ± 8 years, 51% women). Participants with midlife type 2 diabetes were more than twice as likely to make an error on …

Polymeric Nanoparticle As A New Platform For High Removal Of Endotoxins From Biopharmaceutical Solutions, Sidharth Razdan

Doctoral Dissertations

"Presently, approximately one-third of all biopharmaceutical drugs are derived from biological sources like gram-negative bacteria. Gram-negative bacteria like E.coli are much cheaper to cultivate and provide higher biotherapeutic yield compared to mammalian cells. On extracting the useful biotherapeutics from the gram-negative bacterial cells (E.coli), endotoxin present in the bacteria is released in the surrounding media thus contaminating the lifesaving biotherapeutics. Application of the endotoxin-contaminated therapeutics to humans or animals can cause serious health issues like septic shock, tissue injury and ultimately death. Hence, thorough purification of biotherapeutics before parenteral application is necessary. Although there are multiple methods for …

Synthesis, Characterization, And Assessment Of A Ceo2@Nanoclay Nanocomposite For Enhanced Oil Recovery, Mohammad Javad Nazarahari, Abbas Khaksar Manshad, Siyamak Moradi, Ali Shafiei, Jagar Abdulazez Ali, S. Mohammad Sajadi, Alireza Keshavarz

Research outputs 2014 to 2021

© 2020 by the authors. Licensee MDPI, Basel, Switzerland. In this paper, synthesis and characterization of a novel CeO2 /nanoclay nanocomposite (NC) and its effects on IFT reduction and wettability alteration is reported in the literature for the first time. The NC was characterized using scanning electron microscopy (SEM), X-ray diffraction (XRD), Fourier-transform infrared spectroscopy (FTIR), thermogravimetric analysis (TGA), energy-dispersive X-ray spectroscopy (EDS), and EDS MAP. The surface morphology, crystalline phases, and functional groups of the novel NC were investigated. Nanofluids with different concentrations of 100, 250, 500, 1000, 1500, and 2000 ppm were prepared and used as dispersants in …

Investigating Optimum Wavelength(S) For Growth Of Lactuca Sativa, L. Using Tunable Led Sources And Developing Thin-Film Filters For Glass Greenhouses, Jacqualine Anne Thomas

Theses: Doctorates and Masters

With the increase in world population, the continued advances in modern greenhouse agriculture and plant growth practices are expected to help overcome the global problem of future food shortages. This research investigates a way to assist in stemming the problem of food shortage by using optimised light irradiation (within the constraints of the experiment) on a sample plant species of lettuce (Lactuca sativa, L.). Whilst lettuce is often grown in hydroponic systems, the current research is conducted in stand-alone pots with hand watering, due to the requirements of health and safety and available resources.

The experiments were designed …

Increasing The Yield Of Lactuca Sativa, L. In Glass Greenhouses Through Illumination Spectral Filtering And Development Of An Optical Thin Film Filter, Jacqualine Anne Thomas, Mikhail Vasiliev, Mohammad Nur-E-Alam, Kamal Alameh

Research outputs 2014 to 2021

With the increase in world population, the continued advances in modern greenhouse agriculture and plant growth practices are expected to help overcome the global problem of future food shortages. The next generation greenhouse design practices will need to address a range of issues, ranging from energy and land use efficiency to providing plant-optimized growth techniques. In this paper, we focus on investigating the optimum irradiation spectra matched to the lettuce species (Lactuca sativa, L.), commonly grown in greenhouse environments, in order to develop low-emissivity glass panes that maximize the biomass productivity of glass greenhouses. This low-emissivity glass passes the solar …

Remediating Soil For Successful Vegetation Establishment Along Nebraska Highways, Shad Mills, Martha Mamo, Walt Schacht, Humberto Blanco-Canqui

Nebraska Department of Transportation: Research Reports

Vegetation along roadsides is important to prevent soil erosion, provide habitat, and filter water running off the road. Vegetation close to the pavement along highways in Nebraska does not readily establish and persist. It is thought that the sodium and bulk density are the driving factors behind the lack of vegetation. After a construction project the shoulder is seeded into the compacted soil, and during winter salts can accumulate in the soil because of deicing agents being used. The purpose of our study was to determine if the sodium and bulk density are the driving factors of the vegetation cover. …

Electrochemical Determination Of Trace Copper Ions In Drinking Water Source, Jing-Ji Peng, Hong Zheng, Yi-Song Zou, Guo-Kun Liu, Dong-Xing Yuan

Journal of Electrochemistry

Aiming at the on-site and quick safety evaluation of the dissolved trace copper ions (Cu²⁺) in drinking water source, an electrochemical detection platform was developed on the basis of the square wave anodic stripping voltammetry using the gold nanoparticles (Au NPs) modified glassy carbon electrode, where Au NPs were deposited via electrochemical cyclic voltammetry. The proposed method displayed the limit of detection as low as 1.3 μg·L^-1 in the linear range of 2 ~ 50 μg·L^-1. The proposed method was applied to the determination of Cu²⁺ in the midstream of Minjiang River, one of …

Characterization Of Phenolic Acids For Incorporation Into Shape Memory Polymer Foams, Jingyi Liu

Theses - ALL

Shape memory polymer (SMP) foams provide a promising new option for hemorrhage control on the battlefield; however, they lack a mechanism to control wound infections that can delay healing processes. Adding phenolic acids (PAs) into SMP foams provides a natural, non-drug option for incorporations of antioxidant and antimicrobial functionalities that may promote healing. However, PAs lack a systemic characterization of their structure/property relationships, and their chemical incorporation into SMP foams may alter their efficacy. In this work, a library of PAs was screened in terms of antioxidant and antimicrobial properties to provide a full understanding of how their structure affects …

The Scaffolds To Guide Fast Angiogenesis, Tianhong Zhou

McKelvey School of Engineering Graduate Student Theses & Dissertations

Angiogenesis is one of the most challenging problems in bone structure repair. The different types of 3D fibrous morphology structure of the extracellular matrix for fasting angiogenesis have been studied. Due to the densely packed constructs and limited porosity of scaffold, the challenge still remains for scaffold fabrication. In this study, we fabricated PCL scaffold with growth factors and cells to guide fast angiogenesis by electro-spinning and electro-spray simultaneously. We developed a technique that electro-spinning encapsulates growth factors OPN(Recombinant Human Osteopontin) and SDF-1α(CXCL12) in poly(lactide-co-glycolide) (PLGA) microspheres into PCL 3D structures scaffold fibers. We also developed a new technique to …

Research On Color Matching Model For Color Qr Code, Fei You Dr., Qingli Zhang Dr., Bruce Welt Prof.

Journal of Applied Packaging Research

Objective To provide a faster and more effective way for consumers to obtain the additional information of product, the color quick response (QR) code is printed on product packaging. To improve decoding accuracy of color QR codes. This paper constructs a color matching model with good performance, which restrains aliasing between two color encoding modules of color QR code. Methods By comparing and analyzing the character of several typical color spaces, the HSV color space was chosen for its favorable attributes. Based on the enlarging capacity principle of color QR code and the maximum rule of relative Euclidean distance, 2 …

Mems 411 Group L (Fall 2019) - Speedy Seeder Final Report, Michael Morrow, Christopher Nelson, Ross Frame

Mechanical Engineering Design Project Class

The Speedy Seeder or ”SS” is a device designed to decrease the time it takes to fill one seed plot by hand to roughly sixteen percent of the time. The customer of the SS is EarthDance Organic Farms School. EarthDance fills a large number of plots each year by placing a seed in every single compartment which come in three sizes: 32, 72, and 128 compartments per plot. Filling a single plot by hand can take roughly 8 minutes. The SS makes filling a plot much easier and faster, allowing a single plot to be filled in under 75 seconds. …

Certification Of Vegetable Oils And Quality Control In The Technological Process Of Production, Ch.A. Bekmurotov

Technical science and innovation

The article reviewed the certification and quality control of the technological process for the production of vegetable oil. There is a procedure for packaging, quality control of products, determination of quality indicators and quality control of finished products. The procedure for using refined cottonseed oil and its varieties, the requirements for placement and storage of vegetable oils, as specified in the regulatory documents, the quality control of vegetable oils and other solutions for cottonseed oil are provided. The gas analyzer shows a solution for measuring the amount of solution by painting the tubes of a mixture of gas and air …

Comparison Study Of Mold Growth Resistance Of Plastic Based Material Flooring (Pbm Flooring) And Ceramic Tile Flooring, Jyothi Rangineni, Jeremy Tzeng

Publications

Clemson University Department of Biological Sciences and Tile Council of North America Product Performance Testing Laboratory evaluated whether ceramic tile and Plastic Based Material (PBM¹) flooring support mold growth when exposed to fungal spores.

Mold grows in moisture-rich environments and requires only minimal sources of nutrition to support growth. It has long been identified to cause damage to buildings and construction materials and its presence in buildings has been connected to many major health concerns with various studies and reviews published on this matter.²

The method used to evaluate mold growth was ASTM G21-96 (2015).³ This …

Blood Glucose Predictor, Jessica Patterson

Electrical Engineering

For my senior project, I perform data analysis using statistical methods to determine body metrics that correlate with blood glucose levels. Working with Dr. Tina Smilkstein, I take repeat measurements from 6 different volunteers to establish trends in bodily metric data. The data taken includes weight, body fat, pulse rate, VO2, blood glucose, blood pressure, hours slept, and quality of sleep. Using these values, I use the program MiniTab to view results.

A few examples of correlations with blood glucose found in this project are:

• Systolic blood pressure for females had a regression line of 124.0 -0.3366*Blood Pressure. This indicates …

Development Of Forward Osmosis Based Separations: A Novel Approach In Membrane Technology, Yu-Hsuan Chiao

Graduate Theses and Dissertations

Tackling the worldwide severe water shortage, the membrane technology is considered to be the most efficient approach and hence, used widely as a cost-effective sustainable solution. “Forward osmosis (FO)” has been the major attention in recent time. FO uses osmotic pressure as the driving force to draw the water passing the membrane and achieve the desired separation performance. In general, it is considered to be a process with tremendous potential to resolve the present-day water shortage with extremely low energy consumption. However, the challenges of membrane and draw solution regeneration associated with FO processes must be conquered prior to their …

Impact Of Metazooplankton Filter Feeding On Escherichia Coli Under Variable Environmental Conditions, Niveen S. Ismail, Brittney M. Blokker, Tyler R. Feeney, Ruby H. Kohn, Jingyi Liu, Vivian E. Nelson, Mariah C. Ollive, Sarah B. L. Price, Emma J. Underdaha

Engineering: Faculty Publications

The fecal indicator bacterial species Escherichia coli is an important measure of water quality and a leading cause of impaired surface waters. We investigated the impact of the filter-feeding metazooplankton Daphnia magna on the inactivation of E. coli. The E. coli clearance rates of these daphnids were calculated from a series of batch experiments conducted under variable environmental conditions. Batch system experiments of 24 to 48 h in duration were completed to test the impacts of bacterial concentration, organism density, temperature, and water type. The maximum clearance rate for adult D. magna organisms was 2 ml h1 organism1. Less than …

Production And Purification Of Basic Fibroblast Growth Factor Fused To Two Collagen Binding Domains Expressed In E. Coli Bl21 Using Flask And Fed-Batch, Hazim Aljewari

Graduate Theses and Dissertations

Delivering effective and non-toxic doses of bioactive materials that can aid in activating tissue regeneration to wounded tissue has proven to be an enormous challenge. This study was designed to produce a potential therapeutic recombinant protein by fusing two collagen binding domains to basic fibroblast growth factors (bFGF) through a collagenase cleavage site linker, so it can release the bFGF in a wound site by the action of this enzyme. The novel fusion protein was expressed in Escherichia coli BL-21 (E. coli) using traditional flask shaker and fed-batch cultivation. Cell lysate was purified by FPLC using Immobilized metal affinity chromatography …

Fluid Shift And Fluid Resuscitation In Burn Patients With The Use Of Bio-Electrical Impedance Spectroscopy To Monitor Fluid Levels, Temitope D. Obielodan

Honors College Theses

The purpose of this research is to explore the current methods of fluid resuscitation and other possible methods of measuring the body fluid levels of burn patients in order to fully understand the fluid increase patterns in the torso area. This will be done primarily by focusing on the concept of bio-electrical impedance spectroscopy to measure the fluid levels only in the human torso area. Three similar tests were carried out by measuring the resistance values after ingesting 500ml of water. This was repeated until a total of 1500ml of water was ingested. It was found that the resistance in …