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Tissue Characterization And Imaging Using Photon Density Waves, Lars O. Svaasand, Bruce J. Tromberg, Richard C. Haskell, Tsong-Tseh Tsay, Michael W. Berns

All HMC Faculty Publications and Research

The optical properties of brain tissues have been evaluated by measuring the phase velocity and attenuation of harmonically modulated light. The phase velocity for photon density waves at 650-nm wavelength has been found to be in the range of 5 to 12% of the corresponding velocity in a nonscattering medium, and the optical penetration depth was in the range 2.9 to 5.2 mm. These results are used to predict the resolution of optical imaging of deep tissue structures by diffusely propagating incoherent photons. The results indicate that structures of a few millimeters in linear dimension can be identified at 10 …

Explicit Asymmetric Bounds For Robust Stability Of Continuous And Discrete-Time-Systems, Zhiqiang Gao, P J. Antsaklis

Electrical and Computer Engineering Faculty Publications

The problem of robust stability in linear systems with parametric uncertainties is considered. Explicit stability bounds on uncertain parameters are derived and expressed in terms of linear inequalities for continuous systems and inequalities with quadratic terms for discrete-time systems. Cases where system parameters are nonlinear functions of an uncertainty are also examined

Analysis Of The Optimum Extraction System Design For The Separation And Purification Of Rare Earths, Kenneth Yeon-Kang Chan

Dissertations

A mathematical model for the liquid-liquid equilibrium of the lanthanide and yttrium oxides in the nitric acid - water - tributyl phosphate (HNO₃-H₂O-TBP) two-phase system has been developed. This model is based on the interrelationships of chemical reactions, phase equilibrium, and material balance. The chemical reactions were modelled using experimentally derived empirical equations for components involved. The distribution coefficients of 16 rare earths which were experimentally determined. by UK Harwell Laboratory were used to evaluate the parameters of this equilibrium model. Once model parameters are determined, material balance for all species in the HNO₃-H …

Investigation Of The Consumer Electronics Bus, Jaesoo Yang

Dissertations

The objectives of this dissertation are to investigate the performance of the Consumer Electronics Bus (CEBus) and to develop a theoretical formulation of the Carrier Sense Multiple Access with Contention Detection and Contention Resolution (CSMA/CDCR) with three priority classes protocol utilized by the CEBus

A new priority channel assigned multiple access with embedded priority resolution (PAMA/PR) theoretical model is formulated. It incorporates the main features of the CEBus with three priority classes. The analytical results for throughput and delay obtained by this formulation were compared to simulation experiments. A close agreement has been found thus validated both theory and simulation …

Investigation Of Reactor Design Parameters Towards Optimizing Biodegradation Of Chlorophenols By Phanerochaete Chrysosporium, Nirupam Pal

Dissertations

The biodegradation of 2,4,6-trichlorophenol (246-TCP) and 2,4,5-trichiorophenol (245-TCP) by Phanerochaete chrysosporium was studied in batch and in continuous systems. Contrary to most of the previous reports from the literature, this study shows that degradation of both TCPs can occur in the absence of any measurable ligninase activity. The microorganism did not retain its degradative ability for more than about two weeks

In order to better understand the degradation process, the individual contributions of both the biomass and the extracellular proteins were studied separately. The results show that neither the biomass nor the extracellular proteins alone can completely degrade 246-TCP, but …

Development Of A Model For An Objective Minimum Resolvable Temperature Difference Characterization Of An Infrared Camera, Christine Issa Mourad

Theses

The parameter almost universally adopted by the infrared community for describing the overall performance of a thermal imaging camera is the minimum resolvable temperature difference. Unfortunately, this criterion is subjectively determined by a group of observers and as a consequence significantly varying results can be obtained at different times. This project describes work on an approach which ultimately aims to replace the observers by a computer-aided installation that simulates the human process by which the MRTD is estimated. The simulation is based on a direct measurement of signal to noise ratio in the image of the target and a determination …

Graphics Interfaced Computer Automated Process Planning, Ajit Nimbvikar

Theses

The work is based on the manufacturing of gears in a Job Shop type production plant. The topic is divided into three sections. 1. design, 2. graphics, and 3. process planning and analysis. A program is developed using Fortran for the iterative design procedure. With basic inputs, the program provides the design of gears with dimensions. During the procedure, the user has to intervene to specify some codes for the selection of materials and other parameters. Second section deals with the graphics utility. It provides a drawing of the gear with dimensions according to the input obtained from the design …

Microstructural Investigation Of Surface Coating Developed By Electro-Spark Deposition Technology, Tianzong Xu

Theses

Electro-spark deposition (ESD) is a pulsed-spark micro-welding process using an electrode to apply a fused, metallurgically bonded coating with an extremely low heat input so as to eliminate thermal distortion or changes in metallurgical structure of the substrate. The deposited surface achieves an exceptionally fine-grained, homogeneous coating that may approach as amorphous structure which is believed to contribute to good wear and corrosion resistance.

In this research, an optical image analysis system, scanning electron microscope with computerized energy dispersive spectrometer, microhardness testing system and computerized surfanalyzer were employed to investigate the microstructure and properties of achieved surface coatings.

As the …

Application Of Imaging Pyrometry For Remote Temperature Measurements, Michael B. Kaplinsky

Theses

The radiometric model of an IR image sensor has been developed. Based on this model, the application of imaging pyrometry to remote temperature measurements has been investigated. This analysis provides the estimation of temperature accuracy achievable by imaging pyrometry in conjunction with a number of radiometric methods. The detection of radiation emitted across the full spectral bandwidth of the imager as well as utilization of narrow-passband filters was analyzed for a target with known emissivity.

The methods of two-wavelength ratio radiometry and multi-wavelength radiometry were considered for the targets with unknown emissivity. The optimal selection of the wavelengths for the …

Modification Of Wear Testing Machine And Study Of Wear Resistance, Ning Li

Theses

In this work a modification of a wear testing machine has been done by designing a new loading mechanism and redesigning different components for a new roller-on-roller wear testing machine. A new loading mechanism containing gearbox frame and load cell assembly have been designed, manufactured and assembled. A pusher of the pushing mechanism is redesigned for better durability. Existing bearing support was redesigned and replaced. All new units of the wear testing machine were lined up and assembled with the machine. Finite element stress analysis for designed frame in the load mechanism was performed by using the Integrate Design Engineering …

Electrochemical Technique For Evaluating Surface Area Of A Treated Copper Foil, Gregory Lea

Opportunities for Undergraduate Research Experience Program (OURE)

In order to increase the strength of copper foil bonded to a polymeric substrate material, a dendritic layer is electrodeposited onto the foil. Treating the foil in this manner causes its surface to be rough and porous, thus providing for better penetration of the adhesive and a greater surface area for bonding. The process is particularly helpful in the manufacturing of printed circuit cards, due to the added bond strength which greatly enhances the durability of the printed circuit cards.

Tape Casting For Lead Zirconate Titanate (Pzt) Multilayer Composites, Jane L. Cochran

Opportunities for Undergraduate Research Experience Program (OURE)

Tape casting is a process for making thin flat ceramic pieces by using a scraping blade or "doctor blade" to evenly coat a smooth surface with a slip[ 1 ]. The slip is a mixture of powder, solvent, binder, and other organics which result in a paint like consistency after they are mixed.

The tapes is cast into thicknesses ranging from 1 to 50 mils on a smooth surface , which is usually made of Mylar, and left to dry.

Once the tape has dried it can be cut into several different shapes and sizes, and then fired to form …

Dynamics Of Ultrashort Pulse Generation And Amplification In Dye Lasers, Shuanghua Jiang

Dissertations and Theses

The dynamics of ultrashort pulse generation and amplification in dye lasers is studied in this dissertation. In particular, we have developed general semiclassical models for ultrashort pulse dye laser amplifiers and oscillators. These models start from Maxwell's equation for the electric field and density matrix equations for the active laser medium. A finite coherence time or phase memory time of the molecular wave functions, a finite vibrational relaxation time for the lower electronic state of the dye laser transition, an isotropic molecular orientational distribution, and an arbitrary pump polarization are all taken into account. Based on these models, specific topics …

Design Of Holographic Optical Interconnections, Su-Chiou Tsay

Theses

Optics have many advantages over electronics in interconnecting systems together, particularly in terms of higher transmission speed and better immunity to noise. Optical interconnections may be used at all levels of computing: for local area networks, between processors or memory elements, and between boards.

In this thesis, we attempted to generate a new type of computer hologram for optical interconnection purposes. The holographic method is very promising whenever integrated optical interconnections are considered. In this research we have tried two designs: the shortest path grating design which can maximize the global output energy and the single input maximum output grating …

Ultrastructural, Cytochemical, And Immunocytochemical Studies On Bone And Its Interfaces, M. D. Mckee, A. Nanci

Cells and Materials

Bone cells possess the ability to synthesize, secrete and direct the assembly and maintenance of extracellular matrix to form a functionally rigid and/or weight-bearing mineralized tissue complex, the skeleton. The skeleton not only supports and protects the organs and tissues of the body, but also acts as a mineral ion reservoir for maintaining systemic calcium balance (calcium homeostasis). The remarkable biological precision necessary for the formation, turnover and constant adaptation of bone to external mechanical forces is a dynamic process requiring a coordinated cellular effort relying on a variety of cell-cell and cell-matrix/mineral interactions. Indeed, cell-matrix interfaces found in a …

Summer 1993, 90.9 Wmpg Fm

WMPG Program Guides

WMPG program guide for Summer 1993.

Spring 1993, 90.9 Wmpg Fm

WMPG Program Guides

WMPG Program Guide, Spring 1993.

Immunohistochemical Investigation Of Tracks Left By The Migration Of Fibroblasts On Titanium Surfaces, Y. Abiko, D. M. Brunette

Cells and Materials

Fibronectin, a major attachment protein, has been thought to be involved in pathway guidance, a process in which cells migrate along specific pathways within a tissue during development. Fibroblasts exhibit the phenomenon of contact guidance, the tendency of cells to be guided in their direction of migration by the shape of the substratum. The purpose of our study was to test the hypothesis that fibronectin tracks are deposited by fibroblasts moving on smooth and grooved titanium surfaces.

The study was carried out on human gingival fibroblasts which were plated onto both smooth and grooved titanium substrata using medium containing either …

Experimental Induction Of Odontoblast Differentiation And Stimulation During Preparative Processes, H. Lesot, C. Begue-Kirn, M. D. Kubler, J. M. Meyer, A. J. Smith, N. Cassidy, J. V. Ruch

Cells and Materials

In vivo implantation experiments have shown that ethylenediaminetetraaceticacid(EDTA)-soluble frac tions of dentin stimulate reparative dentinogenesis . When isolated embryonic dental papillae were cultured in the presence of these dentin constituents, odontoblast cytological and functional differentiation could be initiated and maintained in the absence of an enamel organ. These effects were attributed to the presence of TGF-/1- related molecules [TGF-/11 or bone morphogenetic protein -2a (BMP-2a)] which had to be used in combination with an EDT A-soluble fraction of dentin in order to specifically affect competent preodontoblasts . These EDT A-soluble constituents present in dentin could be replaced by heparin or …

Calcification Of Liposomes And Red Cell Ghosts In Vitro And In Vivo, Kookmin M. Kim

Cells and Materials

To study the role of membranous phospholipids in calcification , liposomes made of phosphatidylserine and red blood cell ghosts (RBCG) prepared from rat and canine blood were incubated in minimal essential medium (MEM) with 2 .5 mM (mmol/l) calcium and 1.3 mM phosphate, pH 7.4 (MEM-2.5), and homologous (rat) or autologous (canine) plasma filtrate (serum) at 37°C for up to 1 week. Calcification was determined by electron probe microanalysis , electron diffraction, and depletions of calcium and phosphate from MEM-2.5 and sera. Liposomes and rat-RBCG incubated in MEM-2.5 and serum calcified in a week, whereas isolated collagen and elastin did …

Are Long Term Cryopreservation And Patency Of Vein Allograft Truly Achievable?, G. Pasquinelli, G. L. Faggioli, P. Preda, M. Vici, G. N. Martinelli, R. Giardino, M. D'Addato, J. J. Ricotta

Cells and Materials

Despite extensive experimental work, neither the effect of long term cryopreservation on vein graft architecture nor the failure of alloveins due to graft rejection have yet been investigated. Herein, we investigated ultrastructurally: a) the integrity of rabbit jugular veins following 1, 2 and 3 months of cryopreservation; b) the outcome of the three-month cryopreserved vein auto- and allografts after 1 month of implantation in the rabbit carotid artery; and c) the immunologic response to cryopreserved vein allografts with and without seeded autologous endothelium. Prior to implantation, the cryopreserved rabbit veins were well-maintained except for endothelial cell damage. Following implantation, the …

Osteoclastic Resorption Of Calcium Phosphate Ceramic Thin Films, J. E. Davies, G. Shapiro, B. F. Lowenberg

Cells and Materials

Sub-micron calcium phosphate ceramic thin films were formed by vertically dipping transparent quartz plates in a particulate sol-gel suspension. Primary adult rat bone marrow cell populations were cultured on the ceramic thin films in conditions known to allow the differentiation of cells of the osteoclast lineage. Monitoring the cultures for periods of 11 to 28 days revealed the creation of resorption lacunae in the thin films by multinucleate cells. Some cultures were heated at 42 °C overnight to remove adherent cells; using bright field light microscopy (LM), after staining with silver nitrate, the degree of resorption could be easily assessed. …

Preferential Adhesion Of Urethral Bacteria From A Mixed Population To A Urinary Catheter, Gregor Reid, Christina Tieszer

Cells and Materials

The ability of uropathogens to adhere to catheters and subsequently colonize the urinary mucosa leads to urinary tract infections which afflict a large patient population. In vitro studies were carried out whereby Escherichia coli Hu734 (water contact angle 12 °) and Enterococcus faecalis (19°) were found to be highly adhesive to silicone latex urinary catheters. The addition of one of four Lactobacillus sp., with water contact angles ranging from 19-105 °, to the suspending fluid caused a 60-86% reduction in pathogen adhesion with a significant effect against E. coli, the organism most commonly found to infect the urinary tract. …

Mineralization Of An Axially Aligned Collagenous Matrix: A Morphological Study, D. L. Christiansen, F. H. Silver

Cells and Materials

Bone can be described as a highly ordered composite of type I collagen integrated with an inorganic mineral phase. In vitro models of bone mineralization using collagenous substrates have been reported in the literature. This study reports an in vitro system of mineralized reconstituted collagen fibers, with aligned fibrillar substructure. The collagen fibers were mineralized in a double diffusion chamber saturated with respect to calcium and phosphate. The morphology and ultrastructure of the mineral precipitate were evaluated as a function of the pH of the incubating media. Brushite crystal was observed at acidic pH. Large rectangular crystals formed at pH …

Phenotypic Characterization Of Copper-Resistant Mutants Of Methylosinus Trichosporium Ob3b, M. (Mark) W. Fitch, D. W. Graham, R. G. Arnold, S. K. Agarwal, P. Phelps, G. E. Speitel, G. Georgiou

Civil, Architectural and Environmental Engineering Faculty Research & Creative Works

Cultures of Methylosinus Trichosporium OB3b Grown in the Presence of Very Low Concentrations of Copper Synthesize a Soluble Methane Monooxygenase (SMMO) that Efficiently Catalyzes the Oxidation of Trichloroethylene and Other Organic Pollutants. Recently, We Isolated Five M. Trichosporium OB3b Mutants that Express SMMO Activity When Grown in the Presence of Elevated Copper Concentrations (P. A. Phelps, S. K. Agarwal, G. E. Speitel, Jr., and G. Georgiou, Appl. Environ. Microbiol. 58:3701-3708, 1992). Here We Show that, in Contrast to the Results for the Wild-Type Cells, the Addition of Copper to Mutant Cultures Grown on Methane and Nitrate as the Nitrogen Source …

Characterization Of Bovine Osteoclasts On An Ionomeric Cement In Vitro, D. H. Szulczewski, U. Meyer, K. Moller, U. Stratmann, S. B. Doty, D. B. Jones

Cells and Materials

Primary bovine osteoclasts were obtained by an outgrowth method from bovine periosteum and cultured for 7 days on an ionomeric cement for biomaterial testing. Osteoclasts cultured on slices of bovine bone and on glass microscope cover-slides served as a control. The cells were characterised as osteoclasts by a number of tests. Osteoclasts showed positive staining for tartrate resistant acid phosphatase and reactivity with the antibodies 13C2 and 23C6, which react with the alphachain of the vitronectin receptor. Addition of salmon calcitonin to the culture medium led to sudden cessation of lamellipodial activity. The cells resorbed bone by making pits. In …

A Scanning Electron Microscope Study Of The Ultrastructural Organization Of Bone Mineral, F. B. Bagambisa, U. Joos, W. Schilli

Cells and Materials

Scanning electron microscopy (SEM) was used to study samples of lamellar bone at magnifications typical for the published transmission electron micrographs, to gain more insight into the three-dimensional ultrastructure of bone mineral. Untreated (whole bone) samples allowed an assessment of the degree of mineralization. Deproteinized samples revealed the ultrastructural form and organization of bone apatite to be a function of the extent to which collagen fibers were imbibed with mineral. Numerous parallel formations reminiscent of troughs, gutters, or furrows, pierced and traversed the mineralization front. These troughs showed varying diameters identifiable with collagen fibers, and were separated from one another …

Attachment Kinetics And Differentiations Of Osteoblasts On Different Biomaterials, U. Meyer, D. H. Szulczewski, K Moller, H. Heide, D. B. Jones

Cells and Materials

Primary bovine osteoblast cell cultures were used to study the response of osteoblasts ton three different biomaterials, ionomeric cement (IC), tri-calcium phosphate poly-L-lactic acid composite foil (TCP) and poly-L-lactate-polycitric acid composite foil (PLA). The cells which grew on the materials produced all typical bone matrix proteins and were osteoblast-Iike as shown by immuno-staining. Analysis of the cell attachment kinetics revealed significant differences within the first 7 hours between the various materials. The best rate of cell attachment was found on the IC surface, followed by the TCP and then the PLA surface. The kinetics of attach ment appeared to be …

Cell-Degradation Of Calcium Phosphate Ceramics, P. Frayssinet, N. Rouquet, F. Tourenne, J. Fages, D. Hardy, G. Bonel

Cells and Materials

Calcium phosphate ceramics are used in bone surgery under different forms: dense or porous ceramic s as bone substitute, thin ceramic coatings on metallic implants as an osseointegration enhancer. Their degradation depends on their physico-chemical properties and particularly on their chemical composition. Natural calcium phosphates of bone are degraded by mononuclear or multinuclear cells and the extracellular matrix induces the differentiation of the degrading-cells. Hydroxyapatite, which is one of the most used calcium phosphates , is known as a low degradation material. However, the histological analysis of implanted HA-materials both in animals and in humans showed that a cellular degradation …

Analysis Of The Bony Interface With Various Types Of Hydroxyapatite In Vitro, J. D. De Bruijn, J. S. Flach, K. De Groot, C. A. Van Blitterswijk, J. E. Davies

Cells and Materials

Rat bone marrow cells, capable of forming bonelike tissue, were cultured on three types of plasma sprayed hydroxyapatite that differed in degree of crystallinity from 15%, 43% to 69%. The interface between the mineralized extracellular matrix and the hydroxyapatite was studied with scanning and transmission electron microscopy. At the onset of bone formation, calcium and phosphorous-rich afibrillar globules, with a diameter varying from 0.2 to 0.8 μm, were produced and deposited onto the different substrata. These globules subsequently fused to form a homogeneous layer to which collagen fibres became anchored. Individual globules could be distinguished on the non-degrading 69 % …