Digital Commons Network^™

Use Of Combinatorial Genetic Libraries To Humanize N-Linked Glycosylation In The Yeast Pichia Pastoris, Byung-Kwon Choi, Piotr Bobrowicz, Robert C. Davidson, Stephen R. Hamilton, David Kung, Huijuan Li, Robert Miele, Juergen Nett, Stefan Wildt, Tillman Gerngross

Dartmouth Scholarship

The secretory pathway of Pichia pastoris was genetically re-engineered to perform sequential glycosylation reactions that mimic early processing of N-glycans in humans and other higher mammals. After eliminating nonhuman glycosylation by deleting the initiating alpha-1,6-mannosyltransferase gene from P. pastoris, several combinatorial genetic libraries were constructed to localize active alpha-1,2-mannosidase and human beta-1,2-N-acetylglucosaminyltransferase I (GnTI) in the secretory pathway. First, >32 N-terminal leader sequences of fungal type II membrane proteins were cloned to generate a leader library. Two additional libraries encoding catalytic domains of alpha-1,2-mannosidases and GnTI from mammals, insects, amphibians, worms, and fungi were cloned to generate catalytic domain libraries. …

Micro Goniometer For Scanning Probe Microscopy, Ravi F. Saraf

Department of Chemical and Biomolecular Engineering: Patents

A goniometer for performing scanning probe microscopy on a substrate surface is disclosed. The goniometer has a cantilever, having a cantilevered end and a supported end and a tip disposed at the cantilevered end of the cantilever. The goniometer also has a block disposed at the supported end of the cantilever. The block has at least one pair of piezoelectric layers, a pair of electrodes disposed about each individual piezoelectric layer such that varying a potential difference applied between the individual electrodes of a pair of electrodes causes the corresponding piezoelectric layer to deform, and a first insulating material disposed …

2003 - The Eighth Annual Symposium Of Student Scholars

Symposium of Student Scholars Program Books

The full program book from the Eighth Annual Symposium of Student Scholars, held on April 18, 2003. Includes abstracts from the presentations and posters.

The Pseudo-Rigid-Body Model For Dynamic Predictions Of Macro And Micro Compliant Mechanisms, Scott Marvin Lyon

Theses and Dissertations

This work discusses the dynamic predictions of compliant mechanisms using the Pseudo-Rigid-Body model (PRBM). In order to improve the number of mechanisms that can be modeled, this research develops and identifies several key concepts in the behavior of beam segments where both ends are fixed to a rigid body (fixed-fixed flexible segments). A model is presented, and several examples are discussed. The dynamic behavior of several compliant segments is predicted using the PRBM and the results are compared to finite element analysis and experimental results. Details are presented as to the transient behavior of a typical uniform rectangular cross section …

Method Of Preparing A Composite Polymer And Silica-Based Membrane, Dibakar Bhattacharyya, Stephen M. Ritchie, Leonidas G. Bachas, Jamie A. Hestekin, Subhas K. Sikdar

Chemical and Materials Engineering Faculty Patents

A method for preparing a chemically activated or polyamino acid functionalized membrane includes the steps of permeating a silica-based membrane with a solution of silane and a solvent so as to react methoxy groups of the silane with silanol groups of the membrane to incorporate epoxide groups and attaching a polyamino acid to the membrane by reacting a terminal amine group of the polyamino acid with one of the epoxide groups on the membrane.

Preparing And Regenerating A Composite Polymer And Silica-Based Membrane, Dibakar Bhattacharyya, Stephen M. Ritchie, Leonidas G. Bachas, Jamie A. Hestekin, Subhas K. Sikdar

Chemical and Materials Engineering Faculty Patents

A method for preparing and regenerating a chemically activated or polyamino acid functionalized membrane includes the steps of permeating the silica-based membrane with a solution of silane and a solvent so as to react methoxy groups of the silane with silanol groups of the membrane to incorporate epoxide groups and attaching a polyamino acid to the membrane by reacting a terminal amine group of the polyamino acid with one of the epoxide groups on the membrane. The membrane is regenerated after metal entrapment by utilizing helix-coil properties of polyamino acids.

Reconstruction Of The Temperature Profile Along A Blackbody Optical Fiber Thermometer, David Gary Barker

Theses and Dissertations

A blackbody optical fiber thermometer consists of an optical fiber whose sensing tip is given a metallic coating. The sensing tip of the fiber forms an isothermal cavity, and the emission from this cavity is approximately equal to the emission from a blackbody. Standard two-color optical fiber thermometry involves measuring the spectral intensity at the end of the fiber at two wavelengths. The temperature at the sensing tip of the fiber can then be inferred using Planck's law and the ratio of the spectral intensities. If, however, the length of the optical fiber is exposed to elevated temperatures, erroneous temperature …

Providing Central And East European Engineering Students With International Experience, Radu Chisleag

Enhancement of the Global Perspective for Engineering Students by Providing an International Experience

Historically, the academic life in Romania as well as in the other Central and East European countries had strong connections with Western Europe and some connections with the rest of the world. These connections were almost entirely forbidden by the communist soviet type political power imposed to these countries, following the Second World War. The 1989 anticommunist revolution in Central and East Europe opened the way for re-establishing contacts with the western World, offering Romanians and particularly to the students of the "POLITEHNICA" University in Bucharest, opportunities to get International Experience. Due, mainly, to the activity of some promoters, without …

Mediterranean Engineering Schools' Network (Rmei), Zile Soilihi

Enhancement of the Global Perspective for Engineering Students by Providing an International Experience

No abstract provided.

Acuta Enews April 2003, Vol. 32, No. 4

ACUTA Newsletters

In This Issue

ACUTA Ruth A. Michalecki Leadership Award

Quantum Encryption........................ Jeanne Jansenius, Univ. of the South

New Toot Turns Students into Technicians....................... Carol Everett Oliver, Everett Comm.

Lead Us Not lnto Network Complication............................. Kevin Tanzillo, Dux Public Relations

Expanded Eligibility for .EDU Domains

Cell Phone Numbers May Soon Be Available via 411 Directory Assistance

ACUTA Board Report........................ John Bradley, Rensselaer Polytechnic institute

DC Update............................. Whitney Johnson, Retired, Northern Michigan Univ.

Progress Report on Telephone Number Database.............................Jeri Semer, CAE, Executive Director

Welcome New Members

The Airline Quality Rating 2003, Brent D. Bowen, Dean Headley, Uno Aviation Institute

Faculty Books and Monographs

UNOAI Report 03-1

The Airline Quality Rating (AQR) was developed and first announced in early 1991 as an objective method of comparing airline quality on combined multiple performance criteria. This current report, the Airline Quality Rating 2003, reflects monthly Airline Quality Rating scores for 2002. AQR scores for the calendar year 2002 are based on 15 elements that focus on airline performance areas important to air travel consumers.

The Airline Quality Rating 2003 is a summary of month-by-month quality ratings for the 10 largest U.S. airlines operating during 2002. Using the Airline Quality Rating system of weighted averages and monthly …

Gender Differences In Preference For Learning Environment Among Aviation Education Students, Eric M. Moyer

Master's Theses - Daytona Beach

This study investigated whether differences existed between sex, male and female, for the preference of three different syllabi describing three different learning environments. Learning environments consisted of collaborative, and individual, with the individual sub-divided into competitive, and individual while co-varying participants for credit hours. 264 surveys were administered to students in freshman, sophomore, junior, and senior classes in order to collect preference, and demographic data. The surveys were presented as three fictional syllabi differing only in class grading format, and a paragraph on the instructional philosophy of the professor. Instructional philosophies described the proposed environment of the class by enforcing …

Design And Fabrication Of A Microscale Joule -Thomson Refrigerator, Lionel Gamini Hewavitharana

Doctoral Dissertations

A simple thermodynamic, heat transfer, and fluid flow model was developed for microscale Joule-Thomson refrigerators (JT devices). For a given geometry, the model predicted that the cooling capacity of the refrigerator increased with the inlet refrigerant pressure. The effectiveness of the JT device also increased with the inlet pressure, and the heat exchanger channel length. At a constant inlet pressure, the effectiveness, and the refrigeration capacity of a given JT device increased as the aspect ratio of heat exchanger channels was increased. For nitrogen refrigerant, the model predicted that it was possible to obtain approximately 250 mW of refrigeration capacity …

The Effectiveness Of Monitor-Based Augmented Reality Paradigms For Learning Space-Related Technical Tasks, Christopher Michael Opalenik

Master's Theses - Daytona Beach

Currently today there are many types of media that can help individuals learn and excel in the on going effort to acquire knowledge for a specific trait or function in a workplace, laboratory, or learning facility. Technology has advanced in the fields of transportation, information gathering, and education. The need for better recall of information is in demand in a wide variety of areas. Augmented reality (AR) is a technology that may help meet this demand. AR is a hybrid of reality and virtual reality (VR) that uses the three-dimensional location viewed through a video or optical see-through media to …

Inside Unlv, Gian Galassi, Jennifer Vaughan, Fred Bachhuber

Inside UNLV

No abstract provided.

Application Of Remote Sensing And Geographic Information System Methods For Land Using Difference, Bangun Muljo Sukojo, Diah Susilowati

Makara Journal of Technology

Application of Remote Sensing and Geographic Information System Methods for Land Using Difference. Land using difference analysis has been done using remote sensing and Geographic Information System (GIS) methods. Identification of land using difference was conducted using map overlaying process of 1990s (digitized scalling 1:50.000) and 1997s land using map (interpreted from Landsat TM (Thematic Mapper) Image 1997) with UTM (Universal Transverse Mercator) coordinate. Image enhancement was done through histogram equalization with supervised classification devided into 7 classes: rice field, settlement, dry field, industry, pond, sport field and bush. Land using difference and river pollution BOD (Biological Oxygen Demand), COD …

Sculpting: An Improved Inside-Out Scheme For All Hexahedral Meshing, Kirk S. Walton

Theses and Dissertations

Generating all hexahedral meshes on arbitrary geometries has been an area of important research in recent history. Hexahedral meshes have advantages over tetrahedral meshes in structural mechanics because they provide more accurate results with fewer degrees of freedom. Many different approaches have been used to create all-hexahedral meshes. Grid-based, inside-out, or superposition meshing all refer to a similar meshing approach that is a very common mesh generation technique. Grid-based algorithms provide the ability to generate all hexahedral meshes by introducing a structured mesh that bounds the complete body modeled, marking hexahedra to define an interior and exterior mesh, manipulating the …

2003 (Spring) Ensi Informer Magazine, Morehead State University. Engineering Sciences Department

ENSI Informer Magazine Archive

The ENSI Informer Magazine published in the spring of 2003.

Parallel Implementation Of A Face Recognition [Sic] System Based On Modular Pca Approach, Rajkiran Gottumukkal

Electrical & Computer Engineering Theses & Dissertations

This thesis describes research in automated methods for the recognition of human faces. The research is driven by the need to design a method, which would ensure high accuracy under the conditions of facial expression, illumination and pose variations. The resulting method is able to cope with uncontrolled nature of facial expression, illumination and head rotations. The main novelty of this work is the idea that some of the local facial features do not vary even when the facial expression, illumination and pose vary. This idea is applied to the existing principle component analysis lPCA) method to arrive at a …

Bs News

Building Services Engineering

No abstract provided.

Screw Dislocation Interacting With Interface And Interfacial Cracks In Piezoelectric Bimaterials, Xiangfa Wu, Steve Cohn, Yuris A. Dzenis

Department of Engineering Mechanics: Faculty Publications

Interface and interfacial cracks interacting with screw dislocations in piezoelectric bimaterials subjected to antiplane mechanical and in-plane electrical loadings are studied within the framework of linear piezoelectricity theory. Straight dislocations with the Burgers vector normal to the isotropic basal plane near the interface or interfacial crack are considered. The dislocations are characterized by a discontinuous electric potential across the slip plane and are subjected to a line-force and a line-charge at the core. An explicit solution for the screw dislocation in piezoelectric bimaterial with straight interface is found based on the solution of a similar problem for infinite homogenous medium. …

Aviation Safety And Security: A Call For Educating The Flying Public, Mariann Elizabeth Abbonizio

Doctoral Dissertations and Master's Theses

Aviation safety and security: A call for educating the flying public, is an account and testimonial to the problem of the lack of aviation safety and security knowledge by the flying public in the National Airspace System (NAS). It discusses the confusion over both new (post 9-11-01) and old (pre 9-11-01) security regulations as well as basic passenger safety recommendations and advice that could possibly save hundreds of lives. It will discuss the need for a public education campaign regarding aviation safety and security for the American flying public, and point to past public educational campaigns in different realms as …

Microreactor System With Immobilized Enzyme On Polydimethylsiloxane (Pdms) Polymers, Zonghuan Lu

Doctoral Dissertations

Microsystems, specifically microreactors, open the gate to new, improved analytical techniques while offering many advantages for a large number of applications in chemical engineering, pharmacy, medicine, and biotechnology. This study explored the feasibility of fabrication of microreactors using polydimethylsiloxane (PDMS) as a support for enzyme immobilization. Urease enzyme was used for catalyzing the conversion of urea to ammonia.

PDMS (polydimethylsiloxane) is a silicone-based elastomeric polymer. Traditional micromanufacturing technology was employed for reactor mold fabrication. The mold was fabricated based on photolithography techniques, and SU-8 photoresist was used to construct reactor structure templates. The resulting silicon-wafer based reactor molds were then …

Studies On The Use Of Carbon Waste Generated From Fertiliser Plant In Waste Water Treatment, Indra Deo Mall, Sanjay Tewari, Nripendra Singh

Civil, Architectural and Environmental Engineering Faculty Research & Creative Works

Carbon waste generated from fertiliser plant has been used for adsorption studies for the removal of chromium, zinc and nickel and COD from industrial effluent from phenol formaldehyde plant, polyester plant, sugar plant. Removal to the extent of 91.40, 86.8, 93.8 percent was achieved at the initial concentration of 10 mg/1 for Cr(VI), Ni(TI) and Zn(II) respectively using carbon waste as adsorbent Removal of these metals were found to be in order of Zn(II) > Cr(VI) > Ni(II). In a multi cationic solution containing these three metals, Cr(VI) adsorbed preferentially over Ni(II) and Zn(II). COD removal of 67.51%, and 86.4 % was …

Utilisation Of Bagasse Fly Ash And Carbon Waste From Fertiliser Plant For Treatment Of Pyridine And 3-Picoline Bearing Wastewater, Indra Deo Mall, Sanjay Tewari, Nripendra Singh, Indra Mani Mishra

Civil, Architectural and Environmental Engineering Faculty Research & Creative Works

The present work explores the possibility of utilizing sugarcane bagasse fly ash and carbon waste obtained from sugar mills and nitrogenous fertilizer plants, respectively for the removal of pyridine and 3-picoline from waste water. The adsorbing capacity of both adsorbents has been compared with that of activated carbon. Batch studies were conducted to see the effect of contact time, adsorbent dose, initial concentration and pH on the removal of pyridine and 3-picoline. Equilibrium data were fitted with Langmuir and Freundlich isotherms. Adsorptive capacities were found to be in the order: activated carbon > carbon waste > bagasse fly ash. Adsorption was found …

An Approach To Concept Development For Compliant Mechanisms Possessing High Coefficients Of Restitution, Brandon H. Woolley

Theses and Dissertations

The design of structures and mechanisms subject to impact loading has historically involved designing in such a way as to minimize damage induced by the impact. This has historically been accomplished by absorbing and dissipating the energy of the impact. However, in some applications it is desirable to harness the energy and return it to the impacting object to maximize the coefficient of restitution (COR), resulting in large rebound velocities. The use of traditional rigid-body mechanisms to achieve high-COR mechanisms is limited by issues of friction, durability, poor strain-energy distribution and others. Compliant mechanisms do not possess the same limitations …

Voice Activated Display Of American Sign Language For Airport Security, Glenn Lancaster, Karen Alkoby, Jeff Campen, Roymieco Carter, Mary Jo Davidson, Dan Ethridge, Jacob Furst, Damien Hinkle, Bret Kroll, Ryan Layesa, Barbara Loeding, John Mcdonald, Nedjla Ougouag, Jerry Schnepp, Lori Smallwood, Prabhakar Srinivasan, Jorge Toro, Rosalee Wolfe

Visual Communications and Technology Education Faculty Publications

Conference proceedings from the Technology and Persons with Disabilities Conference-2003.

California State University at Northridge, Los Angeles, CA March 17-22, 2003.

Political Authority And Social Cognitions On The War On Terrorism With Global Reach: Airport Security, Terrorism Contingent On A United States-Led Attack On Iraq, Smallpox Vaccinations, Ibpp Editor

International Bulletin of Political Psychology

This article presents analyses of three common social cognitions embraced by many representatives of political authority concerning aspects of the war on terrorism with global reach.

Channel Probing For An Indoor Wireless Communications Channel, Brandon Hunter

Theses and Dissertations

The statistics of the amplitude, time and angle of arrival of multipaths in an indoor environment are all necessary components of multipath models used to simulate the performance of spatial diversity in receive antenna configurations. The model presented by Saleh and Valenzuela, was added to by Spencer et. al., and included all three of these parameters for a 7 GHz channel. A system was built to measure these multipath parameters at 2.4 GHz for multiple locations in an indoor environment. Another system was built to measure the angle of transmission for a 6 GHz channel. The addition of this parameter …

On-Chip Actuation Of Compliant Bistable Micro-Mechanisms, Michael S. Baker

Theses and Dissertations

Two compliant bistable micro-mechanisms have been developed which can be switched in either direction using on-chip thermal actuation. The energy storage and bistable behavior of the mechanisms are achieved through the elastic deflection of compliant segments. The pseudo-rigid-body model was used for the compliant mechanism design, and for analysis of the large-deflection flexible segments. To achieve on-chip actuation, the mechanism designs were optimized to reduce their required rotation, allow them to be switched using linear-motion thermal actuators. The modeling theory and analysis are presented for several design iterations. Each iteration was successfully fabricated and tested using either the MUMPs or …