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Automated System For The Characterization Of The Biomechanical Properties Of Human Soft Tissue, Michael R. Abraham

Mechanical and Aerospace Engineering Theses - Archive

Vaginal prolapse is a common condition that affects a large number (approximately 3.3 million in the US) of women, mainly above the age of 50 years. The condition is characterized by the weakening and breakdown of the pelvic support structures such as muscles and connective tissues, leading to the dislodging and prolapse of pelvic organs. Current examinations of the pelvic tissue are manually performed by the physician who assesses its rigidity. These exams are subjective and offer qualitative rather than quantitative results of the state of the pelvic tissue. In this research, a complete system was conceptualized and designed to …

Mechanism Of Interaction Of Peptide Modified Nanoparticles With Porphyromonas Gingivalis., Ankita Jain

Electronic Theses and Dissertations

Studies suggest that P. gingivalis functions as a keystone pathogen and interacts with primary colonizers in the supragingival biofilm such as S. gordonii. This interaction contributes to the initial colonization of the oral cavity by P. gingivalis and thus represents a potential target for therapeutic intervention. We have identified a peptide (BAR) derived from the streptococcal SspB protein that functions to inhibit P. gingivalis adherence to S. gordonii. In addition, we showed that nanoparticles (NPs) functionalized with BAR inhibit this interaction more potently than free soluble peptide, possibly by promoting interaction with P. gingivalis at higher valency than …

Hydrodynamics Of Swimming Microorganisms In Complex Fluids, Gaojin Li

Open Access Dissertations

Swimming motion of microorganisms, such as spermatozoa, plankton, algae and bacteria, etc., ubiquitously occurs in nature. It affects many biological processes, including reproduction, infection and the marine life ecosystem. The hydrodynamic effects are important in microorganism swimming, their nutrient uptake, fertilization, collective motions and formation of colonies. In nature, microorganisms have evolved to use various fascinating ways for locomotion and transport. Different designs are also developed for the locomotion of artificial nano- and microswimmers. In this study, we use several different computational models to investigate the behavior of microswimmers.

Microorganisms typically swim in the low Reynolds number regime, where inertia …

Examination Of Pseudomonas Fluorescence As A Recombinant Expression Host: Cloning, Expression, And Chromatography, Ahmed K.Ali Elmasheiti

Graduate Theses and Dissertations

In an effort to expand the pool of bacterium useful for biotechnology applications, Pseudomonas fluorescens, a common gram negative microbe, was examined for its ability to function in a recombinant setting. P. fluorescens is ubiquitous in nature and was initially identified as a soil bacterium found in dirt and is typically associated with plant material. Past literature indicates that it shared characteristics common to Escherichia coli and Bacillus subtilis, including simple growth conditions and potential cloning vectors, providing motivation to look into both the upstream and downstream characteristics of this bacterium. First, it was demonstrated that P. fluorescens could be …

A Portable And Automatic Biosensing Instrument For Detection Of Foodborne Pathogenic Bacteria In Food Samples, Zhuo Zhao

Graduate Theses and Dissertations

Foodborne diseases are a growing public health problem. In recent years, many rapid detection methods have been reported, but most of them are still in lab research and not practical for use in the field. In this study, a portable and automatic biosensing instrument was designed and constructed for separation and detection of target pathogens in food samples using nanobead-based magnetic separation and quantum dots (QDs)-labeled fluorescence measurement. The instrument consisted of a laptop with LabVIEW software, a data acquisition card (DAQ), a fluorescent detector, micro-pumps, stepper motors, and 3D printed tube holders. First, a sample in a syringe was …

Functional 3-D Cellulose And Nitrocellulose Paper-Based, Microfluidic Device Utilizing Elisa Technology For The Detection/Distinction Between Hemorrhagic And Ischemic Strokes, Alicia Leanne Holler

Master's Theses

The purpose of this thesis project is to demonstrate and evaluate an enzyme-linked immunosorbent assay (ELISA) on a paper microfluidic device platform. The integration of ELISA technology onto paper microfluidic chips allows for a quantitative detection of stroke biomarkers, such as glial fibrillary acidic protein (GFAP). Dye experiments were performed to confirm fluid connectivity throughout the 3D chips. Several chip and housing designs were fabricated to determine an optimal design for the microfluidic device. Once this design was finalized, development time testing was performed. The results confirmed that the paper microfluidic device could successfully route fluid throughout its channels at …

Diversity And Impacts Of Mining On The Non-Volant Small Mammal Communities Of Two Vegetation Types In The Brazilian Amazon, Natália Carneiro Ardente, Átilla Colombo Ferreguetti, Donald Gettinger, Pricila Leal, Ana Cristina Mendes-Oliveira, Fernanda Martins-Hatano, Helena Godoy Bergallo

Harold W. Manter Laboratory of Parasitology: Faculty Publications

The CarajaÂs National Forest contains some of the largest iron ore deposits in the world. The majority of the minerals are found below a plant community known as Savana Metalo fila, or ªCangaº, which represents only 3% of the landscape within the CarajaÂs National Forest (CNF). The aim of our study was to understand the diversity of community of non-volant small mammals in the two predominant vegetation types: Ombrophilous Forest and Canga, and to examine how mining impacts these communities. Sampling was conducted from January 2010 to August 2011 in 11 sampling sites divided by the total area of Canga …

Pepper Mild Mottle Virus As A Surrogate For Enteric Viruses: Implications For Assessing Water Quality, Erin Michelle Symonds

USF Tampa Graduate Theses and Dissertations

Less than 10% of the world’s domestic wastewater is disinfected prior to discharge into surface waters; therefore, human exposure to diverse wastewater-related pathogens results in millions of cases of illness each year. Among the enteric pathogens, viruses represent an important group of emerging pathogens and are frequently the cause of food- and water-borne outbreaks of illness. Although the World Health Organization and many government agencies mandate the use of bacterial indicators to identify poor microbial water quality, it is well known that these indicators poorly correlate with fecal pollution contamination events and risk of disease. The field of public health-related …

Technology Trajectory Mapping Using Data Envelopment Analysis: The Ex-Ante Use Of Disruptive Innovation Theory On Flat Panel Technologies, Dong-Joon Lim, Timothy R. Anderson

Engineering and Technology Management Faculty Publications and Presentations

In this paper, we propose a technology trajectory mapping approach using Data Envelopment Analysis (DEA) that scrutinizes technology progress patterns from multidimensional perspectives. Literature reviews on technology trajectory mappings have revealed that it is imperative to identify key performance measures that can represent different value propositions and then apply them to the investigation of technology systems in order to capture indications of the future disruption. The proposed approach provides a flexibility not only to take multiple characteristics of technology systems into account but also to deal with various tradeoffs among technology attributes by imposing weight restrictions in the DEA model. …

Clinical Narrative Classification Using Discriminant Word Embeddings With Elm, Paula Lauren, Guangzhi Qu, Feng Zhang, Amaury Lendasse

Engineering Management and Systems Engineering Faculty Research & Creative Works

Clinical Texts Are Inherently Complex Due to the Medical Domain Expertise Required for Content Comprehension. in Addition, the Unstructured Nature of These Narratives Poses a Challenge for Automatically Extracting Information. in Natural Language Processing, the Use of Word Embeddings Are an Effective Approach to Generate Word Representations (Vectors) in a Low Dimensional Space. in This Paper We Use a Log-Linear Model (A Type of Neural Language Model) and Linear Discriminant Analysis with a Kernel-Based Extreme Learning Machine (Elm) to Map the Clinical Texts to the Medical Code. Experimental Results on Clinical Texts Indicate Improvement with Elm in Comparison to Svm …

Flight Physician - October, 2016, Civil Aviation Medical Association

Browse all Civil Aviation Medical Association Newsletters

A twenty-seven page newsletter of the Civil Aviation Medical Association. The newsletter provided news about civil aviation medicine and information related to the organization.

Multi-Sector Analysis Of The Progress And Challenges Of Soil-Transmitted Helminth Eradication In Rural Udaipur, Jessica Ellis

Independent Study Project (ISP) Collection

India is home to 240 million children currently at risk of Soil-Transmitted Helminthiasis, which is spread via unclean water, soil, and food, and causes acute pain and malnutrition. While acknowledging the research debates over WASH and mass drug administration, this study seeks to identify the systemic multidisciplinary issues affecting STH elimination efforts in rural Udaipur. Twenty-three interviews were conducted in villages, government departments, NGOs, and medical sectors to identify the challenges within each approach to deworming, and their experiences with multi-sectoral collaboration. The most recurring issues across disciplines were need for invested local leadership, increase in health literacy of adults, …

Lab-On-A-Chip Nucleic-Acid Analysis Towards Point-Of-Care Applications, Varun Lingaiah Kopparthy

Doctoral Dissertations

Recent infectious disease outbreaks, such as Ebola in 2013, highlight the need for fast and accurate diagnostic tools to combat the global spread of the disease. Detection and identification of the disease-causing viruses and bacteria at the genetic level is required for accurate diagnosis of the disease. Nucleic acid analysis systems have shown promise in identifying diseases such as HIV, anthrax, and Ebola in the past. Conventional nucleic acid analysis systems are still time consuming, and are not suitable for point-ofcare applications. Miniaturized nucleic acid systems has shown great promise for rapid analysis, but they have not been commercialized due …

Prediction And Recommendations On The It Leaners' Learning Path As A Collective Intelligence Using A Data Mining Technique, Seong-Yong Hong, Juyun Cho, Yonghyun Hwang

Journal of International Technology and Information Management

With the recent advances in computer technology along with pervasive internet accesses, data analytics is getting more attention than ever before. In addition, research areas on data analysis are diverging and integrating lots of different fields such as a business and social sector. Especially, recent researches focus on the data analysis for a better intelligent decision making and prediction system. This paper analyzes data collected from current IT learners who have already studied various IT subjects to find the IT learners’ learning patterns. The most popular learning patterns are identified through an association rule data mining using an arules package …

Assessing Latrine Use In Rural India: A Cross-Sectional Study Comparing Reported Use And Passive Latrine Use Monitors, Antara Sinha, Corey L. Nagel, Evan A. Thomas, Wolf P. Schmidt, Belen Torondel, Sophie Boisson, Thomas Clasen

Mechanical and Materials Engineering Faculty Publications and Presentations

Although large-scale programs, like India's Total Sanitation Campaign (TSC), have improved latrine coverage in rural settings, evidence suggests that actual use is suboptimal. However, the reliability of methods to assess latrine use is uncertain. We assessed the reliability of reported use, the standard method, by comparing survey-based responses against passive latrine use monitors (PLUMs) through a cross-sectional study among 292 households in 25 villages in rural Odisha, India, which recently received individual household latrines under the TSC. PLUMs were installed for 2 weeks and householders responded to surveys about their latrine use behavior. Reported use was compared with PLUM results …

State Space Analysis Of Dominant Structures In Dynamic Social Systems, Jeremy B. Sato

McKelvey School of Engineering Graduate Student Theses & Dissertations

Many systems involving human relationships are modeled as dynamic systems, as diverse as urban population growth, diffusion of innovations, spread of viruses, and supply chain management. A fundamental assumption is that these systems contain variables which accumulate and deplete over time (people, innovation adoptions, infections, and orders), and whose dynamics are determined by societal rules and human decision making processes. These assumptions allow the system to be formally expressed by ordinary differential equations which are often nonlinear and contain multiple state variables and feedback loops. Analytical methods have been developed to identify the dominant feedback loops which primarily influence the …

Continuous Isolation Of Monocytes Using A Magnetophoretic-Based Microfluidic Chip, Jeff Darabi, Chuan Guo

SIUE Faculty Research, Scholarship, and Creative Activity

Monocytes play an important role in the immune system and are responsible for phagocytizing and degrading foreign microorganisms in the body. The isolation of monocytes is important in various immunological applications such as in-vitro culture of dendritic cells. We present a magnetophoretic-based microfluidic chip for rapid isolation of highly purified, untouched monocytes from human blood by a negative selection method. This bioseparation platform integrates several unique features into a microfluidic device, including locally engineered magnetic field gradients and a continuous flow with a buffer switching scheme to improve the performance of the cell separation process. The results indicate high monocyte …

Bayesian Networks To Assess The Newborn Stool Microbiome, William E. Bennett Jr.

McKelvey School of Engineering Graduate Student Theses & Dissertations

In human stool, a large population of bacterial genes and transcripts from hundreds of genera coexist with host genes and transcripts. Assessments of the metagenome and transcriptome are particularly challenging, since there is a great deal of sequence overlap among related species and related genes. We sequenced the total RNA content from stool samples in a neonate using previously-described methods. We then performed stepwise alignment of different populations of RNA sequence reads to different indices, including ribosomal databases, the human genome, and all sequenced bacterial genomes. Each pool of RNA at each alignment step was subjected to compression to assess …

Stimuli Responsive Fluidics Controls On A Paper-Based Bacterial Detection Platform, Siyu Zhao, Elizabeth Phillips, Jacqueline Linnes

The Summer Undergraduate Research Fellowship (SURF) Symposium

Infectious diseases are the leading causes of death around the world. Point-of-care devices using nucleic acid amplification are sensitive enough to diagnose these diseases, however, often require complex and time-intensive sample preparation steps that are not integrated with the detection process. A rapid, sensitive, and integrated sample-to-result diagnostic device will permit disease treatment planning at the point-of-care. Paper-based detection assays are a promising platform to integrate the sample preparation and detection, with minimal infrastructure, equipment, and user involvement. To integrate sample preparation with detection on paper-based assays, timing and delivery of sample fluid flow needs to be controlled. Here we …

Climate Change Impacts And Implications: An Integrated Assessment In A Lowland Environment Of New Zealand, A. G.E. Ausseil, K. Bodmin, A. Daigneault, E. Teixeira, E. D. Keller, M. U. F. Kirschbaum, L. Timar, A. Dunningham, C. Zammit, S. Stephens, M. Cameron, P. Blackett, G. Harmsworth, B. Frame, A. Reisinger, A. Tait, D. Rutledge

International Congress on Environmental Modelling and Software

New Zealand’s mixed economy includes a range of sectors (e.g. primary production, energy, tourism) that depend heavily on the state of natural resource capital. This makes the economy heavily dependent on future global climate change and the global economic situation. In this paper, we present a framework to evaluate socio-economic and environmental impacts of future climate and land-use change, and give results for one scenario in a typical lowland environment in New Zealand. The future scenario was designed as a combination of global climate and socio-economic assumptions, along with New Zealand-specific policy assumptions. We evaluated the impacts and implications of …

An Integrated Modelling Approach To Climate Change And Malaria Vulnerability Assessments, Esther Achieng Onyango, Oz Sahin, Cordia Chu, Brendan Mackey

International Congress on Environmental Modelling and Software

Malaria is one of the key research concerns in climate change-health relationships. Numerous risk assessments and modelling studies providing evidence that the transmission range of malaria will expand with rising temperatures resulting in adverse impacts on vulnerable communities. This risk is significant in East Africa whereby current research shows an expansion of malaria into the highland areas due to changes in temperature and rainfall. While there exist multiple lines of evidence for the influence of climate change on malaria and the risk posed to vulnerable communities, there is insufficient understanding of the complexity of factors influencing the spread of the …

Design And Implementation Of Integrated Surveillance And Modelling Systems For Climate- Sensitive Diseases, Michael C. Wimberly, Justin Davis, Geoffrey M. Henebry, Michael B. Hildreth, Yi Liu, Christopher L. Merkord

International Congress on Environmental Modelling and Software

Climatic variations can have a multitude of impacts on human health, ranging from the direct impacts of extreme heat events to indirect effects on the vectors and hosts that transmit a multitude of infectious diseases. Infectious disease surveillance has traditionally focused on monitoring human cases, and in some instances tracking population sizes and infection rates of arthropod vectors and zoonotic hosts. For climate-sensitive diseases, there is potential to strengthen surveillance and obtain early indicators of future outbreaks by monitoring environmental risk factors using broad-scale sensor networks that include earth-observing satellites as well as ground stations. We highlight the opportunities and …

Flight Physician - July, 2016, Civil Aviation Medical Association

Browse all Civil Aviation Medical Association Newsletters

A twenty-one page newsletter of the Civil Aviation Medical Association. The newsletter provided news about civil aviation medicine and information related to the organization.

Three-Dimensional Printing And Nanotechnology For Enhanced Implantable Materials, Karthik Kumar Tappa

Doctoral Dissertations

Orthopedic and oro-maxillofacial implants have revolutionized treatment of bone diseases and fractures. Currently available metallic implants have been in clinical use for more than 40 years and have proved medically efficacious. However, several drawbacks remain, such as excessive stiffness, accumulation of metal ions in surrounding tissue, growth restriction, required removal/revision surgery, inability to carry drugs, and susceptibility to infection. The need for additional revision surgery increases financial costs and prolongs recovery time for patients. These metallic implants are bulk manufactured and often do not meet patient's requirements. A surgeon must machine (cut, weld, trim or drill holes) them in order …

Development And Evaluation Of Custom Prosthetic Devices For A Companion Animal Utilizing Additive Manufacturing., Benjamin Joseph Cahill

Electronic Theses and Dissertations

BACKGROUND AND SIGINIFICANCE: Few options exist for companion animals in need of prosthetic devices. With the rise of rapid prototyping technology, the availability and customization of prosthetic devices for individual companion animals is now a viable and cost effective alternative to the current options of a peg leg prosthetic, or wheel-assisted prosthetic device. The goals of this study were to describe the (1) specific needs and (2) biomechanics of a feline with bilateral thoracic limb amputation, (3) develop custom prosthetic devices utilizing rapid prototyping technology, and (4) describe the biomechanics of a feline with bilateral thoracic limb amputation using the …

Differential Association Of Vitronectin And Fibronectin With Glass And Electrospun Fibers Of A Poly (D-Lysine) /Poly (Acrylic Acid), Syed Muhammad Sohaib Zafar Zafar

USF Tampa Graduate Theses and Dissertations

Proteins represent major constituent of the extracellular matrix which plays an important role in the formation, maintenance and remodeling of tissues, this project focuses on adsorption of two specific serum proteins fibronectin (FN) and vitronectin (VTN) responsible for mediating cell matrix interaction through integrin binding, tripeptide Arg-Gly-Asp (RGD) sequence found in these protein features are recognized by αβV₃ integrin which ultimately helps in clot formation.

Vaccination As A Tool To Reduce Antimicrobial Resistance Worldwide, Bernard Vallat, Elisabeth Erlacher

Vaccine Technology VI

Antimicrobial resistance is a global human and animal health concern that is influenced by the non-appropriate use of antimicrobial agents in both human and veterinary medicine, as well as in the plant sector. To combat antimicrobial resistance, the World Organisation for Animal health (OIE) has developed science-based intergovernmental standards and guidelines covering terrestrial animals and aquaculture.

The OIE also contributed to the development of the WHO Global Action Plan on Antimicrobial Resistance, adopted in 2015 by the World Health Assembly. The 180 Member Countries of the OIE expressed their support to this plan of action through a Resolution, …

How To Deliver New Vaccines Under Very Short Timelines: The Zapi Project, Jean-Christophe Audonnet

Vaccine Technology VI

The ZAPI project is the first One Health project in the European Innovative Medicine Initiative (IMI) program. Its objective is to define and validate unique methodologies that can be applied when new zoonotic infectious diseases will occur in Europe or other regions of the world. The project is supporting an entirely new approach for “anticipating the unexpected”, and is defining new ways to alleviate the technical constraints which are typically encountered with the classical vaccine development processes.

The ZAPI project involves a total of 20 partners, from academic, biotech, and industry origin. One important feature of ZAPI is that it …

Controlled, Pulsatile Release Of Thermostabilized Inactivated Polio Vaccine From Plga-Based Microspheres, Stephany Y. Tzeng, Rohiverth Guarecuco, Kevin J. Mchugh, Evan M. Rosenberg, Yingying Zeng, Robert Langer, Ana Jaklenec

Vaccine Technology VI

Many vaccines, such as the inactivated polio vaccine (IPV), must be administered in several doses for full efficacy. Because patient access is a major challenge for vaccination efforts in developing countries, administering multiple doses per patient is impractical in those areas. Single-administration vaccines would greatly improve efforts to vaccinate populations in Third World countries, and the World Health Organization (WHO) Expanded Program for Immunization describes an ideal vaccine as one that is heat-stable, requires only one shot, and is easy to administer. Although already existing technologies, such as microspheres composed of poly(lactic-co-glycolic acid) (PLGA), are able to encapsulate …

Vaccines: Reaching For Higher Branches After The Low Hanging Fruit Has Been Picked, Michael G. Kurilla

Vaccine Technology VI

Vaccines represent one of the most significant advances by medicine in addressing infectious diseases being responsible for the elimination of a few and the virtual eradication of several other scourges. In addition, medicine is also beginning to appreciate the potential applicability of vaccines beyond infectious diseases to areas such as oncology. During any emerging infectious disease public health event, availability of a vaccine figures prominently in the assessment of the capability to address the threat most effectively. A more rapid deployment of new vaccines would enable more effective management of emerging threats. Efforts to streamline manufacture and production of vaccines …