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Bibliometric Survey On Impact Of Sound Therapy On Blood Pressure And Covid-19., Vaishali Rajput, Preeti Mulay

Library Philosophy and Practice (e-journal)

The current situation of spread of Corona Virus is really worth to worry and critical. As we are moving from stage 2 to stage 3, it is really our duty and right to prevent its spread effectually, as its threat to life of every individual. As per the expert opinion from Health Organizations, the elder people are most at risk of Corona infection. But if the people with high blood pressure or high cholesterol fail to take their prescribed medication, then they are also prone to various infections, due to low immunity levels. Along with regular medication, Sound Therapy is …

Engineering A Switchable Nanosystem For Customizable Therapeutics, David Diebold, Hanzhe Chen

Bioengineering Senior Theses

Exosomes are nanovesicles that are naturally secreted by mammalian cells in vivo for intercellular communications. Due to their inherent targeting ability, exosomes have a potential for therapeutic applications. However, due to their physiological derivation, the isolation of engineered exosomes has been a major obstacle to their therapeutic application, and successful disease-targeting has been difficult to control. Recently, we have developed an exosome technology that borrows from switchable Chimeric Antigen Receptor (sCAR) T-Cell Therapy and Strep-tag engineering to overcome these obstacles. Here, we describe the development of a de novo method to produce genetically modified exosomes with switchable targeting ability and …

Microfluidic Liquid Biopsy For Cancer Prognosis, Brendan Heap, Anthony Ramirez Guerrero, Sam Nichols

Bioengineering Senior Theses

Leukemia is a deadly and common cancer, especially in children and adolescents. The misdiagnosis and unexpected complications during the treatment are some factors that increase the mortality rate of leukemia. The goal of our project was to create a device that would quickly and accurately assess these complications. While there are existing tests that can perform a single test for either metastasis or sepsis, there are none that can test for both simultaneously and rapidly. We propose to modify and combine some of these existing microfluidic designs as well as create a new component to perform a combinatorial assessment. While …

Occurrence And Persistence Of Antibiotics Administered To Cattle In A Newly Established Feedlot, Brittany Trejo

Department of Environmental Engineering: Theses and Other Student Research

The common practice of using therapeutic antibiotics in livestock farming is a worldwide phenomenon. Over the last decade, there has been a growing concern of antibiotics entering the environment via animal manure. Similar studies have focused on the occurrence and biological effects of antibiotics in land-applied animal feedlots; however, limited research has been conducted on the occurrence and persistence of antibiotics in animal feedlots. A study was conducted to investigate the occurrence and persistence of four injected antibiotics (ceftiofur enrofloxacin, florfenicol, and tulathromycin) and two continuously fed antibiotics (monensin and tylosin) in feedlot sediment, runoff, and sediment runoff. For antibiotics …

Identification Of Snps Associated With Multiple Antibiotic Resistance Genes In Eight Clinical Variants Of Enterococcus Faecium., Krishna S. Karia

USF Tampa Graduate Theses and Dissertations

The evolution of multi drug resistant (MDR) bacteria has been well documented these last decade, suggesting that resistance to antibiotics is most commonly associated to single nucleotide polymorphism (SNPs) mutation. To this aim, several molecular methods have been performed to elucidate SNPs in the increasing number of resistant microorganisms. Early detection of MDR bacteria has become of prime necessity in order to combat the disease and ensure public health. Therefore, this research was conducted to screen for SNP mutations correlated to drug resistance in some nosocomial MDR bacteria strains donated from Moffitt Cancer Center (MCC) hospital. Blood, stool, urine, and …

Evaluating The Applicability Of Biostimulated Calcium Carbonate Precipitation To Stabilize Clayey Soils, Md Touhidul Islam, Bhaskar C.S. Chittoori, Malcolm Burbank

Civil Engineering Faculty Publications and Presentations

Clayey soils with medium to high plasticity are prevalent in several parts of the world, causing billions of dollars in damage annually to various civil infrastructures. Several ground-improvement techniques can be employed to counteract this issue. However, these methods are impractical in certain situations and unsustainable in others due to their economic and environmental impacts. Microbial-induced calcite precipitation (MICP) could provide a more sustainable alternative. Researchers have successfully used MICP to alter specific geotechnical properties of sands and silts. This research investigates the applicability of MICP via biostimulation to treat clayey soils with low to high plasticity. The goal is …

The Effect Of Aeration Rate And Free-Floating Carrier Media On The Emission Of Bacillus Globigii In Bioaerosols, Andrew J. Owens

Theses and Dissertations

Aerosols produced by turbulent mechanical mixing and bubble aeration at Waste Water Treatment Plants (WWTPs) become bioaerosols with the entrainment of biological materials. Bioaerosols become a public health risk when human pathogens are present. This study evaluated bioaerosols containing Bacillus globigii (BG) spores, and the effects that aeration rate and the addition of Free-Floating Carrier Media (FFCM) had on the amount of BG spores collected following aerosolization. A series of laboratory-scale experiments investigated two different sizes of floating polystyrene spheres as FFCM and four different aeration rates. When the differences in compared aeration rates were sufficiently large, a positive correlation …

Sustainable, Alginate-Based Sensor For Detection Of Escherichia Coli In Human Breast Milk, Nicholas Kikuchi, Margaret May, Matthew Zweber, Jerard Madamba, Craig M. Stephens, Unyoung Kim, Maryam Mobed-Miremadi

Bioengineering

There are no existing affordable diagnostics for sensitive, rapid, and on-site detection of pathogens in milk. To this end, an on-site colorimetric-based sustainable assay has been developed and optimized using an L₁₆ (5⁴) Taguchi design to obtain results in hours without PCR amplification. To determine the level of Escherichia coli (E. coli) contamination, after induction with 150 µL of breast milk, the B-Per bacterial protein extraction kit was added to a solution containing an alginate-based microcapsule assay. Within this 3 mm spherical novel sensor design, X-Gal (5-Bromo-4-Chloro-3-Indolyl β-_D-Galactopyranoside) was entrapped at a concentration of 2 …

A Bibliometric Survey On The Diagnosis Of Plant Leaf Diseases Using Artificial Intelligence, Rutuja Rajendra Patil, Sumit Kumar Dr

Library Philosophy and Practice (e-journal)

Due to uncertain environmental conditions such as untimely rainfall, hailstorms, draught, fog the agriculture sector faces huge loss in crop yield. One of the biggest reason is plant leaf diseases. Therefore the need arises to diagnose the plant leaf diseases beforehand so that the diseases could be avoided and crop yield loss could be minimized. The paper represents the bibliometric study of plant leaf disease diagnosis using Artificial Intelligence. The study focuses on 472 scientific documents such as journals, articles, book chapters publicized in various journals. These documents are extracted from Scopus database after querying it with keywords related to …

Benzalkonium Chloride Alters Phenotypic And Genotypic Antibiotic Resistance Profiles In A Source Water Used For Drinking Water Treatment, Katherine R. Harrison, Anthony D. Kappell, Patrick J. Mcnamara

Civil and Environmental Engineering Faculty Research and Publications

Antibiotic resistance is a major public health concern. Triclosan is an antimicrobial compound with direct links to antibiotic resistance that was widely used in soaps in the U.S. until its ban by the U.S. Food and Drug Administration. Benzalkonium chloride (BAC), a quaternary ammonium compound, has widely replaced triclosan in soaps marketed as an antibacterial. BAC has been detected in surface waters and its presence will likely increase following increased use in soap products. The objective of this study was to determine the effect of BAC on relative abundance of antibiotic resistance in a bacterial community from a surface water …

A Hybrid Agent-Based And Equation Based Epidemiological Model For The Spread Of Infectious Diseases, Elizabeth Hunter

Doctoral

Infectious disease models are essential in understanding how an outbreak might occur and how best to mitigate an outbreak. One of the most important factors in modelling a disease is choosing an appropriate model and determining the assump tions needed to create the model. The main research questions this thesis addresses are how do we create a model for the spread of infectious diseases that captures heterogeneous agents without using an inordinate amount of computing power and how can we use that model to plan for future infectious disease outbreaks. We start our work by analysing and comparing equation based …

Sick Buildings, Poor Design And Public Heath, Dalia Shebl Said, Yasmin Shebl Said

Architecture and Planning Journal (APJ)

There is no doubt that there is a close relationship between health problems and housing conditions. According to the World Health Organization (WHO) estimates in developing countries those 2 million early and unexpected deaths, with close to half occurring among children less than 5 years old die from indoor air pollution and bad housing. Housing is an important determinant of health, and substandard housing is a major public health issue. Designing and bad orientation of housing are found to have significant effects on poor respiratory health especially in children, and increase an infectious disease thereby putting them at higher risk …

"When The Enemy Drew Our Attention": Reconsidering Prior Restraint In The Context Of Dual Use Research Of Concern, Caine Caverly

William & Mary Bill of Rights Journal

Through 2016 and 2017, a team led by Canadian virologist David Evans, and funded by an American pharmaceutical company, attempted to synthesize the previously extinct horsepox virus. After just six months and an expenditure of $100,000, the research team was able to successfully construct the virus “using only commercially available information, technology and tools.” In January of 2018, the team went on to publish their information in an American-based journal, PLOS ONE.

The publication was controversial because it included a potential “blueprint” for the synthesis of a genetic strand in the same viral family as the highly lethal, albeit eradicated, …

Business Continuity Planning For A Medium-Sized Hotel Under Covid-19 Outbreak, Arunlak Tangtanapaiboon

Chulalongkorn University Theses and Dissertations (Chula ETD)

Business Continuity Planning is a subjective conceptual framework prominently used in operations related to supply chain, information technology, or governmental organizations. In this research, we have applied the concept of business continuity planning to a service business during the COVID–19 outbreaks, whose aim is to determine the business continuity objective with the maximum operational tolerable period. The proposed framework has been evaluated based on the ANOVA Test of a guest's service questionnaire of a hotel in Rayong, Thailand. We find that, by reducing business operating cost via staff working hours reduction and limiting service functions of the hotel to the …

Holistic Resilience Quantification Framework Of Rural Communities, Amanda Melendez

Theses and Dissertations--Civil Engineering

Communities need to prepare for anticipated hazards, adapt to varying conditions, and resist and recover rapidly from disturbances. Protecting the built environment from natural and man-made hazards and understanding the impact of these hazards helps allocate resources efficiently. Recently, an indicator-based and time-dependent approach was developed for defining and measuring the functionality and disaster resilience continuously at the community level. This computational method uses seven dimensions that find qualitative characteristics and transforms them into quantitative measures. The proposed framework is used to study the resilience of rural communities’ subject to severe flooding events. Harlan County in the Appalachian region is …

Covid-19 And Public Transportation: Current Assessment, Prospects, And Research Needs, Alejandro Tirachini, Oded Cats

Journal of Public Transportation

The COVID-19 pandemic poses a great challenge for contemporary public transportation worldwide, resulting from an unprecedented decline in demand and revenue. In this paper, we synthesize the state-of-the-art, up to early June 2020, on key developments regarding public transportation and the COVID-19 pandemic, including the different responses adopted by governments and public transportation agencies around the world, and the research needs pertaining to critical issues that minimize contagion risk in public transportation in the so-called post-lockdown phase. While attempts at adherence to physical distancing (which challenges the very concept of mass public transportation) are looming in several countries, the latest …

Collagen Production And Niche Engineering: A Novel Strategy For Cancer Cells To Survive Acidosis In Dcis And Evolve, Mehdi Damaghi, Hidetoshi Mori, Samantha Byrne, Liping Xu, Tingan Chen, Joseph Johnson, Nathan D. Gallant, Andriy Marusyk, Alexander D. Borowsky, Robert J. Gillies

Mechanical Engineering Faculty Publications

Growing tumors are dynamic and nonlinear ecosystems, wherein cancer cells adapt to their local microenvironment, and these adaptations further modify the environment, inducing more changes. From nascent intraductal neoplasms to disseminated metastatic disease, several levels of evolutionary adaptations and selections occur. Here, we focus on one example of such an adaptation mechanism, namely, “niche construction” promoted by adaptation to acidosis, which is a metabolic adaptation to the early harsh environment in intraductal neoplasms. The avascular characteristics of ductal carcinoma in situ (DCIS) make the periluminal volume profoundly acidic, and cancer cells must adapt to this to survive. Based on discovery …

Development Of Efficient Waste Management System For Guwahati City, Prantar Jyoti Talukdar

Chulalongkorn University Theses and Dissertations (Chula ETD)

As on today, in view of the exponential increase of population, fast urbanisation, technical advancements etc., globally, the waste management is becoming a subject of great concern. Apart from dealing with waste only, any waste management system has to encompass many other variants, including - environments, pollution, public health & hygiene, local eco-system etc. So, it is difficult to frame a common global policy or a country’s specific policy to meet all the requirements of the local service providers and customers. Preferentially, any waste management system need to be developed as per the requirements of the local endeavours. In this …

Non-Linear Inverse Liquid-Solid Chromatography As A Methodology To Characterize Drug Concentration Losses To Polymeric Materials Used In Body-On-A-Chip Devices For Drug Discovery, Mark Schnepper

Electronic Theses and Dissertations, 2020-2023

Body-on-a-chip and human-on-a-chip systems are currently being used to augment and could eventually replace animal models in drug discovery and basic biological research. However, hydrophobic molecules, especially therapeutic compounds, tend to adsorb to the polymer materials used to create these microfluidic platforms, which may distort the dose-response curves that feed into Pharmacokinetic/Pharmacodynamic (PK/PD) models which translate preclinical data into predictions of clinical outcomes. Adsorption of hydrophobic molecules to these polymer materials needs better characterization. Inverse Liquid-Solid Chromatography paired with a numerical optimization based on the Langmuir model of adsorption was used to characterize the adsorption isotherm parameters of selected drugs …

Statistical And Stochastic Learning Algorithms For Distributed And Intelligent Systems, Jiang Bian

Electronic Theses and Dissertations, 2020-2023

In the big data era, statistical and stochastic learning for distributed and intelligent systems focuses on enhancing and improving the robustness of learning models that have become pervasive and are being deployed for decision-making in real-life applications including general classification, prediction, and sparse sensing. The growing prospect of statistical learning approaches such as Linear Discriminant Analysis and distributed Learning being used (e.g., community sensing) has raised concerns around the robustness of algorithm design. Recent work on anomalies detection has shown that such Learning models can also succumb to the so-called 'edge-cases' where the real-life operational situation presents data that are …

Mechanisms Of Pathogen Inactivation In Wastewater And Pharmaceutical Applications, Christa L. Meingast

Dissertations, Master's Theses and Master's Reports

Infectious diseases are a significant threat to public health. Though society enacts practices to prevent the spread of these dangerous diseases, challenges remain. Therefore, continual advancements in treatment and prevention are required. Wastewater treatment and viral clearance in pharmaceutical applications are two key health measures that prevent the spread of infections.

A low-cost, low-technology biosolids treatment process was developed to improve wastewater treatment by collecting key information on storage temperature, ammonia, volatile solids, moisture content, pH, and pathogen inactivation in biosolids over long-term storage at two wastewater treatment plants located in northern climates of the United States. Inactivation of pathogens …

Detection And Thermal Stabilization Of Virus Based On Surface Properties, Xue Mi

Dissertations, Master's Theses and Master's Reports

Viral diseases take the lives of millions of people each year. The most effective methods to prevent viral disease outbreak are viral detection to reduce contact with viral pathogens and vaccines to prevent disease. To reduce the costs of the detection of viruses and improve vaccine formulation, we explored viral surface properties. The properties we have focused on are viral hydrophobicity and surface charge using chemical force microscopy (CFM). CFM is a single-particle technique that measures the adhesion force of a functionalized atomic force microscopy (AFM) probe, and in this study, a virus covalently bound to a surface. The non-enveloped …

Continuous Viral Vaccine Manufacturing And Viral Detection Strategies, Dylan G. Turpeinen

Dissertations, Master's Theses and Master's Reports

Viruses are responsible for many human diseases that lead to suffering and death. Acquired immunodeficiency syndrome (AIDS), influenza, and coronavirus disease 2019 (COVID-19) have claimed the lives of millions of people. Two methods have been created to reduce the suffering caused by these diseases. The first is the manufacturing of vaccines that prevent disease and the second is the development of virus detection tests that lead to treatment. The work summarized in this dissertation discusses the development of a continuous virus purification process using an aqueous two-phase system for use in vaccine manufacturing. Additionally, two novel virus detection methods were …

Virus-Like Particle Vaccine Manufacturing From Yeast, Ananya Ananya

Dissertations, Master's Theses and Master's Reports

Influenza virus has an intrinsic nature to undergo mutations. Hence, there have been struggles to combat this disease since the time when the worst influenza pandemic first hit in 1918. Vaccines have proved to be effective in controlling the spread of the influenza A virus by providing herd immunity. However, the contemporary design of influenza A virus based on egg and cell-culture is not efficient in tackling influenza A virus transformation. Virus-like particles (VLPs) have established themselves as a potental platform for future vaccine candidates. VLPs have all the credentials to supplant contemporary vaccine designs without compromising on immunogenicity. For …

Harvesting Electrical Energy Produced By Electrogenic Bacteria In Microbial Fuel Cells, Chinedu Ahuchaogu, Kausik Das

Pursue: Undergraduate Research Journal

Cellular respiration is the process by which organic matter oxidizes, and the energy stored in the chemical bonds of the food releases. Normally, cellular respiration occurs inside the mitochondria of cells; however, a unique type of bacteria releases electrons externally. These specialized organisms are called electrogenic bacteria. Our goal is to construct a microbial fuel cell (MFC) with electrogenic bacteria, harvest the external electrons created by cellular respiration, and channel them through an external circuit to generate electricity. Mud soil, which has a high number of electrogenic bacteria in the environment, was used to construct an MFC. In the presence …

Automatic Chest X-Rays Analysis Using Statistical Machine Learning Strategies, Hermann Yepdjio Nkouanga

All Master's Theses

Tuberculosis (TB) is a disease responsible for the deaths of more than one million people worldwide every year. Even though it is preventable and curable, it remains a major threat to humanity that needs to be taken care of. It is often diagnosed in developed countries using approaches such as sputum smear microscopy and culture methods. However, since these approaches are rather expensive, they are not commonly used in poor regions of the globe such as India, Africa, and Bangladesh. Instead, the well known and affordable chest x-ray (CXR) interpretation by radiologists is the technique employed in those places. Nevertheless, …

Virus Purification Framework And Enhancement In Aqueous Two-Phase System, Pratik Umesh Joshi

Dissertations, Master's Theses and Master's Reports

Viral infections regularly pose detrimental health risks to humans. Preventing viral infections through global immunization requires the production of large doses of vaccines. The increasing demand for vaccines, especially during pandemics such as COVID-19, has challenged current manufacturing strategy to develop advanced unit operations with high throughput capability. Over the decade, the upstream processing responsible for synthesizing viral products in cell cultures has shown significant success in yielding high titers of viruses and virus-like particles. The progress in the upstream stage has now shifted the bottleneck to the downstream processing (DSP). Overlooked for decades, the DSP responsible for viral product …

Hsp90 Inhibition And Modulation Of The Proteome: Therapeutical Implications For Idiopathic Pulmonary Fibrosis (Ipf), Ruben Manuel Luciano Colunga Biancatelli, Pavel Solopov, Betsy Gregory, John D. Catravas

Bioelectrics Publications

Idiopathic Pulmonary fibrosis (IPF) is a catastrophic disease with poor outcomes and limited pharmacological approaches. Heat shock protein 90 (HSP90) has been recently involved in the wound-healing pathological response that leads to collagen deposition in patients with IPF and its inhibition represents an exciting drug target against the development of pulmonary fibrosis. Under physiological conditions, HSP90 guarantees proteostasis through the refolding of damaged proteins and the degradation of irreversibly damaged ones. Additionally, its inhibition, by specific HSP90 inhibitors (e.g., 17 AAG, 17 DAG, and AUY-922) has proven beneficial in different preclinical models of human disease. HSP90 inhibition modulates a complex …

Utilizing Virtual High-Throughput Screens To Identify Promising Inhibitors Of Complement Factor C1s, Lyndsey Schmucker

Williams Honors College, Honors Research Projects

A large study was conducted to identify possible drug candidates to treat various diseases using computer algorithms, virtual high-throughput screens, and experimental validation of activity. Since the algorithm has the capability to screen millions of compounds, it is beneficial to pharmaceutical development as it allows a larger pool of compounds to be considered that would have otherwise been overlooked. As part of this larger study, this project attempts to identify drug candidates to treat human complement factor C1, a protein which causes tissue damage when underregulated¹. A series of designed experiments validate candidates and confirm the performance of …

Internet Of Things For Sustainable Human Health, Abdul Salam

Faculty Publications

The sustainable health IoT has the strong potential to bring tremendous improvements in human health and well-being through sensing, and monitoring of health impacts across the whole spectrum of climate change. The sustainable health IoT enables development of a systems approach in the area of human health and ecosystem. It allows integration of broader health sub-areas in a bigger archetype for improving sustainability in health in the realm of social, economic, and environmental sectors. This integration provides a powerful health IoT framework for sustainable health and community goals in the wake of changing climate. In this chapter, a detailed description …