Digital Commons Network^™

A Multi-Function, Disposable, Microfluidic Module For Mutation Detection, Tae Yoon Lee

LSU Doctoral Dissertations

Recognition of point mutations in a codon 12 of the K-ras gene, most frequently observed, is considered to be useful in the early diagnosis of several types of the human cancers. We have developed a multifunction, disposable, microfluidic module which detects low-abundant point mutations in human genomic DNA in modular architecture. Each functional component including a microfluidic PCR reactor, a passive diffusional micromixer reactor, and a microfluidic LDR reactor was separately designed and fabricated. Fluidic interconnects were also developed to make a fluidic passage between the functional components. Polycarbonate substrates were micro-molded, using hot embossing with micro-milled brass mold inserts …

Water And Sanitation Accessibility And The Health Of Rural Ugandans, Jonathan E. Mellor

Dissertations, Master's Theses and Master's Reports - Open

The lack of access to sufficient water and sanitation facilities is one of the largest hindrances towards the sustainable development of the poorest 2.2 billion people in the world. Rural Uganda is one of the areas where such inaccessibility is seriously hampering their efforts at development. Many rural Ugandans must travel several kilometers to fetch adequate water and many still do not have adequate sanitation facilities. Such poor access to clean water forces Ugandans to spend an inordinate amount of time and energy collecting water - time and energy that could be used for more useful endeavors. Furthermore, the difficulty …

An Algorithm For Identifying Novel Targets Of Transcription Factor Families: Application To Hypoxia-Inducible Factor 1 Targets, Yue Jiang, Bojan Cukic, Donald A. Adjeroh, Heath D. Skinner, Jie Lin, Qingxi J. Shen, Bing-Hua Jiang

Electrical & Computer Engineering Faculty Research

Efficient and effective analysis of the growing genomic databases requires the development of adequate computational tools. We introduce a fast method based on the suffix tree data structure for predicting novel targets of hypoxia-inducible factor 1 (HIF-1) from huge genome databases. The suffix tree data structure has two powerful applications here: one is to extract unknown patterns from multiple strings/sequences in linear time; the other is to search multiple strings/sequences using multiple patterns in linear time. Using 15 known HIF-1 target gene sequences as a training set, we extracted 105 common patterns that all occur in the 15 training genes …

A Modular Approach To High Throughput Microsystems, Pin-Chuan Chen

LSU Doctoral Dissertations

A modular high throughput microsystem was developed using microfabrication technology and nucleic acid analysis. The purpose of developing this microsystem is to identify acute infectious disease and prevent contagious outbreak in a matter of time. This microsystem included three major components, a reagent distribution device, a 96 CFPCR array, and a multi-zone thermal system, to efficiently amplify specific DNA fragments to determine the disease status or precisely pinpoint a disease from multiple patients. Polymerase chain reaction (PCR) is the key component in the nucleic acid analysis for disease because it can be used to amplify interested DNA fragments by repeatedly …

The Modification, System Software Development, Validation, And Clinical Trial Of A Novel Ankle Stiffness Testing Device, T. Tyler Clement

LSU Master's Theses

The purpose of this project is to modify, validate, and gather preliminary clinical data on a novel ankle stiffness device that measures stiffness (torque / degree) and range of motion (ROM) of the ankle joint complex (AJC). The initial device was designed by a mechanical engineering design group at Louisiana State University in 2006, but had not been tested on human participants. Clinical evaluation of the ankle joint is important in patients afflicted with diabetes mellitus, Hansen’s disease, or peripheral neuropathies. The combination of peripheral neuropathies, decreased ankle range of motion, and increased stiffness pose a threat to these patients, …

A Fundamental Study Of The Pcr Amplification Of Gc-Rich Dna Templates, T. G. Mamedov, Elsje Pienaar, Scott E. Whitney, Joel R. Termaat, G. Carvill, R. Goliath, Anuradha Subramanian, Hendrik J. Viljoen

Hendrik J. Viljoen Publications

A theoretical analysis is presented with experimental confirmation to conclusively demonstrate the critical role that annealing plays in efficient PCR amplification of GC-rich templates. The analysis is focused on the annealing of primers at alternative binding sites (competitive annealing) and the main result is a quantitative expression of the efficiency (η) of annealing as a function of temperature (T_A), annealing period (t_A), and template composition. The optimal efficiency lies in a narrow region of T_A and t_A for GC-rich templates and a much broader region for normal GC templates. To confirm the …

Workers At Risk: The Unfulfilled Promise Of The Occupational Safety And Health Act, Lynn Rhinehart

West Virginia Law Review

No abstract provided.

Crumb Rubber As A Sorption Medium For Ethylbenzene In Aqueous Solution, Carmen Grayson

All Theses

Scrap tires have always been an environmental concern. In 2005, more than 299 million scrap tires were generated in the U.S., 259 million of which were utilized in the end-use scrap tire markets such as: tire-derived fuel generation, civil engineering applications, and ground rubber applications. The different applications and usages in the scrap tire market have helped utilize up to 87% of annually generated scrap tires in the U.S. Sixteen percent of the scrap tires were utilized in civil engineering applications in 2005 because of their beneficial properties for vibration and sound control, lightweight fill, drainage and collection for underground …

Flight Physician - July, 2008, Civil Aviation Medical Association

Browse all Civil Aviation Medical Association Newsletters

A sixteen page newsletter of the Civil Aviation Medical Association. The newsletter provided news about civil aviation medicine and information related to the organization.

Vaccine Technology Ii: Conference Program, Barry C. Buckland, John G. Auniņš, Paula Marques Alves, Kathrin U. Jansen

Vaccine Technology II

No abstract provided.

Emerging Diseases, Zoonoses And Vaccines To Control Them, Paul-Pierre Pastoret

Vaccine Technology II

No abstract provided.

Vaccine Technology Ii: Abstracts Of Oral Presentations, The Editors

Vaccine Technology II

No abstract provided.

2008 Epa’S Report On The Environment

United States Environmental Protection Agency: Publications

To accomplish its mission, the U.S. Environmental Protection Agency (EPA) must pay close attention to trends in the condition of the nation’s air, water, and land, and to associated trends in human exposure and health and the condition of ecological systems. Data on environmental trends serve two key purposes: they provide valuable input to EPA in developing its strategic outlook and priorities, and they allow EPA and the public to assess whether the Agency is succeeding in its overall mission to protect human health and the environment. EPA prepared this Report on the Environment (ROE) to accomplish these purposes.
In …

Monitoring Of Indoor Relative Humidity Levels In Residential Dwellings: A Sensor Network Application, Lizabeth Lee

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Indoor Air Quality is an increasing concern in the world today. The mere presence of people in a building or residence can significantly alter indoor air quality. Relative humidity over the range of normal indoor temperatures (66 - 80 degrees Farenheit) has been linked both directly and indirectly to various health and structural problems. The purpose of this project was to discover whether residential dwellings might benefit from an indoor humidification system. The project consisted of the deployment of three separate sensor networks consisting of 12 tmote sky modules manufactured by the Moteiv corporation, each equipped with a temperature and …

Santa Clara Magazine, Volume 49 Number 4, Spring 2008, Santa Clara University

Santa Clara Magazine

14 - LET THE SUN SHINE IN By Steven Boyd Saum. In the international Solar Decathlon competition, a team from Santa Clara blazed a dazzling trail from almost-ran to third in the world.

20 - VISIONS FROM THE SIXTIES By Lisa Taggart. It was art that broke all the rules. And now an exhibit at the de Saisset Museum, curated by Santa Clara scholar Andrea Pappas, captures the sense of optimism and energy when the only limits were imagination itself.

26 - JORMA'S JOURNEY By Mark Purdy. With Jefferson Airplane, he helped define the San Francisco sound. With Hot Tuna …

Using Wavelet And Template Analysis To Classify Hand Postures In Unsupervised Daily Activities, Soha Hassan Saleh

Theses

This project's goal was to identify determinants that characterize different types of activities an individual do in daily life, knowing the quality of hand function is essential to plan more effective rehabilitation therapies and treatments for upper limb movement disorders. The first part of the project was Jebsen-Taylor study where healthy individuals and individuals with brain injury performed seven activities classified as precision grasp, cylindrical grasp, and palmar grasp while metacarpal joint angles were measured in real time. The data from those seven activities was used to determine parameters that characterize each type of activity and which might be used …

Performance Evaluation Of Vip Latrines In The Upper West Region Of Ghana , James W. Dumpert

Dissertations, Master's Theses and Master's Reports - Open

The prevalence of Ventilated Improved Pit (VIP) latrines in Ghana suggests that the design must have a high user acceptance. The two key factors attributed to user acceptance of a VIP latrine over an alternative latrine design, such as the basic pit latrine, are its ability to remove foul odors and maintain low fly populations; both of which are a direct result of an adequate ventilation flow rate.

Adequate ventilation for odorless conditions in a VIP latrine has been defined by the United Nations Development Program (UNDP) and the World Bank, as an air flow rate equivalent to 6 air …

Pseudomonas Aeruginosa Infection Of The Auricular Cartilage Caused By “High Ear Piercing”: A Case Report And Review Of The Literature, Henry H. Rowshan, Karen Keith, Dale Baur, Peter Skidmore

United States Army: Publications

Piercing of various parts of the body has gained tremendous popularity among adolescent and adult populations in recent years. There are no exact statistical reports on the number of body piercings being performed in the United States. One study among 481 college students in New York revealed that 42% of men and 60% of women had body piercing. In the past, the majority of complications of ear piercing were associated with the lobule. Torn ear lobes, localized infection, hypertrophic scars, allergic contact dermatitis, and keloid formations were among the most common, with no significant morbidity. However, “high ear piercing,” defined …

Phase 2a Trial Of 0, 1, And 3 Month And 0, 7, And 28 Day Immunization Schedules Of Malaria Vaccine Rts,S/As02 In Malaria-Naive Adults At The Walter Reed Army Institute Of Research, Kent E. Kester, James F. Cummings, Christian F. Ockenhouse, Robin Nielsen, B. Ted Hall, Daniel M. Gordon, Robert J. Schwenk, Urszula Krzych, Carolyn A. Holland, Gregory Richmond, Megan G. Dowler, Jackie Williams, Robert A. Wirtz, Nadia Tornieporth, Laurence Vigneron, Martine Delchambre, Marie-Ange Demoitie, W. Ripley Ballou, Joe Cohen, D. Gray Heppner Jr.

United States Army: Publications

Background: Immunization with RTS,S/AS02 consistently protects some vaccinees against malaria infection in experimental challenges and in field trials. A brief immunization schedule against falciparum malaria would be compatible with the Expanded Programme on Immunization, or in combination with other prevention measures, interrupt epidemic malaria or protect individuals upon sudden travel to an endemic area.
Methods: We conducted an open label, Phase 2a trial of two different full dose schedules of RTS,S/AS02 in 40 healthy malaria-naıve adults. Cohort 1 (n = 20) was immunized on a 0, 1, and 3 month schedule and Cohort 2 (n = 20) on a 0, …

Influenza Immunization And Subsequent Diagnoses Of Group A Streptococcus-Illnesses Among U.S. Army Trainees, 2002–2006, Seung-Eun Lee, Angelia Eick, Michael S. Bloom, John F. Brundage

United States Army: Publications

To assess the association between influenza immunization and subsequent diagnosis of group A streptococcus (GAS)-illness in Army recruits during influenza seasons 2002–2006. A case–control study was employed with cases as trainees with outpatient GAS diagnosis (ICD-9-CM codes: 034.0, 035, 038.0, 041.01, 320.2, 390–392, 482.31) during the influenza season, and controls as trainees with no outpatient GAS diagnosis during the influenza season. Primary exposure was influenza immunization during 1st September to 30th April of each season. Estimated protective effects of influenza immunization against GAS-illness ranged from 50% to 77%. A strong protective effect was suggested for Army trainee influenza immunization on …

Moderate Zinc Restriction Affects Intestinal Health And Immune Function In Lipopolysaccharide-Challenged Mice, Daniel G. Peterson, Angus G. Scrimgeour, James P. Mcclung, Elizabeth A. Koutsos

United States Army: Publications

Zinc (Zn) is an essential nutrient that affects immune function, especially within the digestive system, although the underlying mechanisms are not well understood. This study examined the effects of short-term moderate Zn restriction on intestinal health and immune function in lipopolysaccharide (LPS)-challenged mice through plasma cytokine profiling and histological evaluation of intestinal tissue sections. Adult male mice were fed with a Zn-adequate (40 ppm) or a Zn-marginal (4 ppm) diet for 4 weeks, and then a bacterial challenge was simulated by intraperitoneal injection of LPS (10 μg/g body weight [BW]) or saline (control). BW was recorded weekly, and feed intake …

The Minimum Information About A Genome Sequence (Migs) Specification, Dawn Field, George Garrity, Tanya Gray, Norman Morrison, Jeremy Selengut, Peter Sterk, Tatiana Tatusova, Nicholas Thomson, Michael J. Allen, Samuel V. Angiuoli, Michael Ashburner, Nelson Axelrod, Sandra Baldauf, Stuart Ballard, Jeffrey Boore, Guy Cochrane, James Cole, Peter Dawyndt, Paul De Vos, Claude Depamphilis, Robert Edwards, Nadeem Faruque, Robert Feldman, Jack Gilbert, Paul Gilna, Frank Oliver Glöckner, Philip Goldstein, Robert Guralnick, Dan Haft, David Hancock, Henning Hermjakob, Christiane Hertz-Fowler, Phil Hugenholtz, Ian Joint, Leonid Kagan, Matthew Kane, Jessie Kennedy, George Kowalchuk, Renzo Kottmann, Eugene Kolker, Saul Kravitz, Nikos Kyrpides, Jim Leebens-Mack, Suzanna E. Lewis, Kelvin Li, Allyson L. Lister, Phillip Lord, Natalia Maltsev, Victor Markowitz, Jennifer Martiny, Barbara Methe, Ilene Mizrachi, Richard Moxon, Karen Nelson, Julian Parkhill, Lita Proctor, Owen White, Susanna-Assunta Sansone, Andrew Spiers, Robert Stevens, Paul Swift, Chris Taylor, Yoshio Tateno, Adrian Tett, Sarah Turner, David Ussery, Bob Vaughan, Naomi Ward, Trish Whetzel, Ingio San Gil, Gareth Wilson, Anil Wipat

United States Department of Energy: Publications

With the quantity of genomic data increasing at an exponential rate, it is imperative that these data be captured electronically, in a standard format. Standardization activities must proceed within the auspices of open-access and international working bodies. To tackle the issues surrounding the development of better descriptions of genomic investigations, we have formed the Genomic Standards Consortium (GSC). Here, we introduce the minimum information about a genome sequence (MIGS) specification with the intent of promoting participation in its development and discussing the resources that will be required to develop improved mechanisms of metadata capture and exchange. As part of its …

Cyanobacterial Harmful Algal Blooms: Chapter 38: Integrating Human And Ecological Risk Assessment: Application To The Cyanobacterial Harmful Algal Bloom Problem, Jennifer Orme-Zavaleta, Wayne Munns, Jr.

United States Environmental Protection Agency: Publications

Environmental and public health policy continues to evolve in response to new and complex social, economic and environmental drivers. Globalization and centralization of commerce, evolving patterns of land use (e.g., urbanization, deforestation), and technological advances in such areas as manufacturing and development of genetically modified foods have created new and complex classes of stressors and risks (e.g., climate change, emergent and opportunist disease, sprawl, genomic change). In recognition of these changes, environmental risk assessment and its use are changing from stressor-endpoint specific assessments used in command and control types of decisions to an integrated approach for application in communitybased decisions. …

Cyanobacterial Harmful Algal Blooms: Chapter 15: Cyanotoxins Workgroup Report, Rex Pegram, Andrew Humpage, Brett Neilan, Maria Runnegar, Tonya Nichols, Robert Thacker, Stephan Pflugmacher, Stacey Etheridge, Adam Love

United States Environmental Protection Agency: Publications

The Cyanotoxins Workgroup was charged with the identification and prioritization of research needs associated with: the identification of cyanotoxins; toxicokinetics and toxicodynamics of cyanotoxins; human susceptibility to the toxins; cyanobacterial genetics/omics and factors for inclusion in predictive models of toxin production; and risk reduction from an intentional or accidental release of cyanotoxins. Papers presented for the Cyanotoxins Session of the symposium on toxin types, toxicokinetics, and toxicodyamics (See Humpage this volume), cyanobacterial genetics of toxin production (See Neilan this volume), and parameters related to human risks from cyanobacterial exposure (See Love this volume) set the stage for Cyanotoxins Workgroup discussions. …

Factors Affecting The Spread Of A Bioterrorist Agent Throughout A Building, William A. Cantrell

Inquiry: The University of Arkansas Undergraduate Research Journal

Bioterrorism has become a greater concern for Americans since the 2001 anthrax letters. Recent studies have explored the possibilities of biological attacks, and most deal with possible large-scale attacks. However, there is reason to believe that small-scale attacks are more likely. Even though there have been investigations of the postal delivery system and the spread of bioagents through mail, few if any studies have looked at attack on a single building and the resultant spread form room to room. One particular method of attacking a building would be a single-even release of an aerosol bioagent in the building. This paper …

Acute Respiratory Health Effects Of Air Pollution On Children With Asthma In Us Inner Cities, George T. O'Connor, Lucas Neas, Benjamin Vaughn, Meyer Kattan, Herman Mitchell, Ellen F. Crain, Richard Evans, Iii, Rebecca Gruchalla, Wayne Morgan, James Stout, G. Kenneth Adams, Morton Lippmann

United States Environmental Protection Agency: Publications

Background: Children with asthma in inner-city communities may be particularly vulnerable to adverse effects of air pollution because of their airways disease and exposure to relatively high levels of motor vehicle emissions.

Objective: To investigate the association between fluctuations in outdoor air pollution and asthma morbidity among inner-city children with asthma.

Methods: We analyzed data from 861 children with persistent asthma in 7 US urban communities who performed 2-week periods of twice-daily pulmonary function testing every 6 months for 2 years. Asthma symptom data were collected every 2 months. Daily pollution measurements were obtained from the Aerometric Information Retrieval System. …

Adipocyte Response To Injectable Beads Engineered For Breast Tissue Reconstruction, Katherine Neser

All Theses

It is estimated that over 150,000 patients will be diagnosed with invasive breast cancer in 2007. Patients must undergo a mastectomy or a lumpectomy to remove the cancerous tissue, but only a mastectomy allows breast reconstructive surgery. Due to the limitations of current reconstruction options, new alternatives are being explored. Injectable materials have been suggested for breast tissue reconstruction because of their versatility. Cells cultured on injectable beads form cell carriers that may be mixed with a hydrogel, resulting in a construct that may be injected through a syringe to restore normal tissue mass. This solution offers breast cancer patients …

An In Vitro Evaluation Of Dbm As A Tissue Engineered Scaffold, Stephanie Arnold

All Theses

Over 500,000 bone graft procedures are performed each year in the United States. Bone grafting involves a surgical procedure to replace missing bone. Problems can arise with donor and defect sites during and after surgery, sometimes resulting in poor clinical results. The development and optimization of bone graft substitutes via a tissue engineering approach could markedly improve bone graft surgical outcome. Demineralized bone matrix (DBM), a bone graft material, is currently used in a clinical setting but has variable success rates.
The primary objective of the research presented in this thesis was to assess the cellular activity of D1 mouse …

Biodiesel Forming Reactions Using Heterogneous Catalysis, Yijun Liu

All Dissertations

Biodiesel synthesis from biomass provides a means for utilizing effectively renewable resources, a way to convert waste vegetable oils and animal fats to a useful product, a way to recycle carbon dioxide for a combustion fuel, and production of a fuel that is biodegradable, non-toxic, and has a lower emission profile than petroleum-diesel. Free fatty acid (FFA) esterification and triglyceride (TG) transesterification with low molecular weight alcohols constitute the synthetic routes to prepare biodiesel from lipid feedstocks. This project was aimed at developing a better understanding of important fundamental issues involved in heterogeneous catalyzed biodiesel forming reactions using mainly model …

Sheep Updates 2007 - Part 4, Dean Thomas, Dominique Blache, Dean Revell, Hayley Norman, Phil Vercoe, Zoey Durmic, Serina Digby, Di Mayberry, Megan Chadwick, Martin Sillence, David Masters, R. Silberstein, F. Byrne, P. G. H. Nichols, J. Young, L. Aitkins, M. G. Wilmot, A. J. Rintoul, T. Lambert, D. R. Mcclements, P. Raper, P. Ward, C. Walton, T. York, David Pethick, David Hopkins, Malcolm Mcphee, D. B. Savage, J. V. Nolan, I. R. Godwin, A. Aoetpah, T. Nguyen, N. Baillie, C. Lawler, Ken Geenty, John Smith, Darryl Smith, Tim Dyall, Grant Uphill, John Young, Michael Paton, John Dorrian

Sheep Updates

This session covers eight papers from different authors:

GRAZING

1. The impact of high dietary salt and its implications for the management of livestock grazing saline land, Dean Thomas, Dominique Blache, Dean Revell, Hayley Norman, Phil Vercoe, Zoey Durmic, Serina Digby, Di Mayberry, Megan Chadwick, Martin Sillence and David Masters, CRC for Plant-based Management of Dryland Salinity, Faculty of Natural & Agricultural Sciences, The University of Western Australia, WA.

2. Sustainable Grazing on Saline Lands - outcomes from the WA1 research project, H.C. Norman^1,2, D.G. Masters^1,2, R. Silberstein^1,2, F. Byrne^2,3, P.G.H. Nichols …