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2011 - The Sixteenth Annual Symposium Of Student Scholars

Symposium of Student Scholars Program Books

The full program book from the Sixteenth Annual Symposium of Student Scholars, held on April 26, 2011. Includes abstracts from the presentations and posters.

Accelerating Finite State Projection Through General Purpose Graphics Processing, Thomas Trimeloni

Theses and Dissertations

The finite state projection algorithm provides modelers a new way of directly solving the chemical master equation. The algorithm utilizes the matrix exponential function, and so the algorithm’s performance suffers when it is applied to large problems. Other work has been done to reduce the size of the exponentiation through mathematical simplifications, but efficiently exponentiating a large matrix has not been explored. This work explores implementing the finite state projection algorithm on several different high-performance computing platforms as a means of efficiently calculating the matrix exponential function for large systems. This work finds that general purpose graphics processing can accelerate …

Evaluation Of A Novel Non-Penetrating Electrode For Use In Dna Vaccination, Amy Donate, Domenico Coppola, Yolmari Cruz, Richard Heller

Bioelectrics Publications

Current progress in the development of vaccines has decreased the incidence of fatal and non-fatal infections and increased longevity. However, new technologies need to be developed to combat an emerging generation of infectious diseases. DNA vaccination has been demonstrated to have great potential for use with a wide variety of diseases. Alone, this technology does not generate a significant immune response for vaccination, but combined with delivery by electroporation (EP), can enhance plasmid expression and immunity. Most EP systems, while effective, can be invasive and painful making them less desirable for use in vaccination. Our lab recently developed a non-invasive …

Optimization Of Point-Of-Use Water Treatment Device For Disaster Relief, Margaret June Herzog

Master's Theses

Point-of-use (POU) drinking water treatment is a common method of providing drinking water in disaster relief situations when critical water infrastructure is damaged. In these cases, POU treatment devices can be used to treat local water until relief organizations set up more permanent water provision methods. One such POU technology is PŪR^® Purifier of Water, a combined coagulation/flocculation and disinfection chemical treatment sachet produced by Procter & Gamble. PŪR^® has been shown to treat contaminated water to meet water quality standards and guidelines set by the U.S. EPA for water purifiers and by the World Health Organization and …

Engineering Research 2011, College Of Engineering, Michigan Technological University

Michigan Tech Engineering Magazine

Table of Contents

• Air & Water
• Energy
• Engineered Materials
• Health
• Sensing & Controls
• People
• Research Centers & Institutes

A Detailed And Systematic Investigation Of Personal Ventilation Systems, Jackie Russo

Mechanical and Aerospace Engineering - Dissertations

This research investigates the use of personal ventilation (PV) in a typical office space as a means of contaminant removal from ones breathing zone (BZ). For this work, a validated computational model was developed and used for PV assessment under different scenarios. Experimental data of Khalifa et al. (2009), Ito (2007) and Rim et al. (2009) were used to validate a computational model that is capable of simulating indoor chemical reactions with excellent agreement compared with the experiments. Through the validation process, various computational parameters were determined to be significant for producing accurate results. Grid resolution, geometry, far field BCs, …

Label-Free Biochemical Recognition Using Mems Resonators For Microarray Technology, Babak Tousifar

Electronic Theses and Dissertations

Highly sensitive biosensors capable of detecting and characterizing smallest quantities of cellular and molecular targets are needed in pharmaceutical and medical diagnostics industries. In this work, the importance of biological target recognition specifically proteins through microarray technologies has been discussed and the most successful tools and techniques have been studied. Moreover, a thermally actuated Micro Electro-Mechanical Systems (MEMS) resonator has been demonstrated and fabricated in this work as an accurate, reliable and low cost biotechnology tool. As a proof of concept, amine to epoxide coupling of octadecylamine to functionalized silicon dioxide surface have been shown through resonator frequency monitoring. The …

Safety And Protective Efficacy Of Ina-Inactivated Venezuelan Equine Encephalitis Virus: Implication In Vaccine Development, Anuj Sharma, Paridhi Gupta, Pamela J. Glass, Michael D. Parker, Radha K. Maheshwari

United States Army: Publications

We have previously shown that a hydrophobic alkylating compound, 1,5-iodonaphthyl-azide (INA) can efficiently inactivate the virulent strain of Venezuelan equine encephalitis virus (VEEV), V3000 in vitro. In this study, we have evaluated the safety of INA-inactivated V3000 and V3526 and the protective efficacy of INA-inactivated V3000. INA-inactivated V3000 and V3526 did not cause disease in suckling mice. RNA isolated from the INA-inactivated V3000 and V3526 was also not infectious. Immunization of adult mice with INA-inactivated V3000 induced an anti-VEEV antibody response and protected mice from virulent VEEV challenge. The protective efficacy of INA-inactivated V3000 increased with the use of adjuvants. …

Protective Immunity To Pre-Erythrocytic Stage Malaria, Robert J. Schwenk, Thomas L. Richie

United States Army: Publications

The development of a vaccine against malaria is a major research priority given the burden of disease, death and economic loss inflicted upon the tropical world by this parasite. Despite decades of effort, however, a vaccine remains elusive. The best candidate is a subunit vaccine termed RTS,S but this provides only partial protection against clinical disease. This review examines what is known about protective immunity against pre-erythrocytic stage malaria by considering the humoral and T cell-mediated immune responses that are induced by attenuated sporozoites and by the RTS,S vaccine. On the basis of these observations a set of research priorities …

Clostridium Difficile Infections In Patients With Severe Burns, Scott J. Crabtree, Janelle L. Robertson, Kevin K. Chung, Evan M. Renz, Steven E. Wolf, Duane R. Hospenthal, Clinton K. Murray

United States Army: Publications

With improved survival in burn patients, Clostridium difficile infection (CDI) remains a significant potential complication. The incidence of, risk factors for, and outcomes of CDI in severely burned patients are poorly studied and remain unclear. This study involves retrospective case control and cohort studies using electronic medical records from February 1, 2002 to January 31, 2009 at the US Department of Defense’s only burn unit. Demographic, risk factor, and outcome data were collected for all C. difficile toxin positive patients in the burn, medical, and surgical intensive care units and the hospital’s step down unit along with an additional analysis …

Pamam Dendrimer-Based Therapeutic And Diagnostic Nanodevices, Admira Bosnjakovic

Wayne State University Dissertations

Dendrimers are ideal materials to be used in the emerging field of nanomedicine. Their nanoscale size and high density of functional groups on their peripheries allow them to be used for various biomedical applications. This work exploits dendrimers as drug delivery vehicles and a versatile platform for capturing biomarkers with improved sensitivity and specificity. Hydroxyl terminated poly(amidoamine) dendrimer (PAMAM-OH) was modified with a linker having amine group at the end and conjugated to two drugs, erythromycin (EM) and allopregnanonolne, respectively and evaluated in vitro. Both drugs were provided with a linker having carboxylic group required for conjugation reaction with dendrimer. …

Small Molecule Inhibitors Of Staphylococcus Aureus Rnpa Alter Cellular Mrna Turnover, Exhibit Antimicrobial Activity, And Attenuate Pathogenesis, Patrick D. Olson, Lisa J. Kuechenmeister, Kelsi L. Anderson, Sonja Daily, Karen E. Beenken, Christelle M. Roux, Michelle L. Reniere, Tami L. Lewis, William J, Weiss, Mark Pulse, Phung Nguyen, Jerry W. Simecka, John M. Morrison, Khalid Sayood, Oluwatoyin A. Asojo, Mark S. Smeltzer, Eric P. Skaar, Paul M. Dunman

Department of Electrical and Computer Engineering: Faculty Publications

Methicillin-resistant Staphylococcus aureus is estimated to cause more U.S. deaths annually than HIV/AIDS. The emergence of hypervirulent and multidrug-resistant strains has further amplified public health concern and accentuated the need for new classes of antibiotics. RNA degradation is a required cellular process that could be exploited for novel antimicrobial drug development. However, such discovery efforts have been hindered because components of the Gram-positive RNA turnover machinery are incompletely defined. In the current study we found that the essential S. aureus protein, RnpA, catalyzes rRNA and mRNA digestion in vitro. Exploiting this activity, high through-put and secondary screening assays identified …

Purification Of A Recombinant Heavy Chain Fragment C Vaccine Candidate Against Botulinum Serotype C Neurotoxin [Rbontc(HC)] Expressed In Pichia Pastoris, Michael P. Dux, Jicai Huang, Rick Barent, Mehmet Inan, S. Todd Swanson, Jayanta Sinha, John T. Ross, Leonard A. Smith, Theresa J. Smith, Ian Henderson, Michael M. Meagher

United States Army: Publications

A purification process for the manufacture of a recombinant C-terminus heavy chain fragment from botulinum neurotoxin serotype C [rBoNTC(H_c)], a potential vaccine candidate, has been defined and successfully scaled-up. The rBoNTC(H_c) was produced intracellularly in Pichia pastoris X-33 using a three step fermentation process, i.e., glycerol batch phase, a glycerol fed-batch phase to achieve high cell densities, followed by a methanol induction phase. The rBoNTC(H_c) was captured from the soluble protein fraction of cell lysate using hydrophobic charge induction chromatography (HCIC; MEP HyperCel™), and then further purified using a CM 650M ion exchange chromatography …

Modeling And Surveillance Of Pandemic Influenza Outbreaks, Diana Prieto

USF Tampa Graduate Theses and Dissertations

Pandemic outbreaks are unpredictable as to their virus strain, transmissibility, and impact on our quality of life. Hence, the decision support models for mitigation of pandemic outbreaks must be user-friendly and operational, and also incorporate valid estimates of disease transmissibility and severity. This dissertation research is aimed at 1) reviewing the existing pandemic simulation models to identify their implementation gaps with regard to usability and operability, and suggesting research remedies, 2) increasing operability of simulation models by calibrating them via an epidemiological model that estimates infection probabilities using viral shedding profiles of concurrent pandemic and seasonal influenza, and 3) developing …

Viability Study Of A Residential Integrated Stormwater, Graywater, And Wastewater Treatment System At Florida's Showcase Green Envirohome, Matthew Allen Goolsby

Electronic Theses and Dissertations

The subject of water scarcity and the rate of water consumption has become popular topics over the last few decades. It is possible that society may consume or contaminate much of the remaining readily available water if there is not a paradigm shift. This deep rooted concern has prompted investigations to identify alternative water use and treatment methods. Within this report, information is presented from the use of innovative water harvesting and on-site sewage treatment and disposal systems (OSTDS) at Florida’s Showcase Green Envirohome (FSGE.net), while also addressing low impact development (LID) practices. FSGE is a residential home that demonstrates …

A Validation Of A Prototype Dry Electrode System For Electroencephalography, Jason Monnin

Browse all Theses and Dissertations

Current physiologically-driven operator cognitive state assessment technology relies primarily on electroencephalographic (EEG) signals. Traditionally, gel-based electrodes have been used; however, the application of gel-based electrodes on the scalp requires expertise and a considerable amount of preparation time. Additionally, discomfort can occur from the abrasion of the scalp during preparation, and the electrolyte will also begin to dry out over extended periods of time. These drawbacks have hindered the transition of operator state assessment technology into an operational environment. QUASAR, Inc., (San Diego, CA) has developed a prototype dry electrode system for electroencephalography that requires minimal preparation. A comparison of the …

The Development Of Silver Nanoparticles As Antiviral Agents, John Christopher Trefry

Browse all Theses and Dissertations

Silver nanoparticles (AgNPs) have received tremendous attention for their antimicrobial properties; however, many gaps in knowledge exist. To address these issues, three research objectives were examined. The first objective hypothesized AgNPs can be size selected and concentrated via tangential flow ultrafiltration. The second objective hypothesized a high-throughput method could be developed to screen nanoparticle antiviral-activity and cytotoxicity simultaneously. The third objective hypothesized AgNPs inhibit viruses by preventing viral entry.

For objective one, a tangential flow ultrafiltration scheme was tested on AgNPs synthesized via the Creighton Colloid method. AgNPs were analyzed via transmission electron microscopy. In objective two, an HIV-1 vector …

Comparative Manufacture And Cell-Based Delivery Of Antiretroviral Nanoformulations, Shantanu Balkundi, Ari S. Nowacek, Ram S. Veerubhotla, Han Chen, Andrea Martinez-Skinner, Upal Roy, R. Lee Mosley, Georgette Kanmogne, Xinming Liu, Alexander V. Kabanov, Tatiana Bronich, Joellyn Mcmillan, Howard E. Gendelman

Nebraska Center for Biotechnology: Faculty and Staff Publications

Nanoformulations of crystalline indinavir, ritonavir, atazanavir, and efavirenz were manufactured by wet milling, homogenization or sonication with a variety of excipients. The chemical, biological, immune, virological, and toxicological properties of these formulations were compared using an established monocyte-derived macrophage scoring indicator system. Measurements of drug uptake, retention, release, and antiretroviral activity demonstrated differences amongst preparation methods. Interestingly, for drug cell targeting and antiretroviral responses the most significant difference among the particles was the drug itself. We posit that the choice of drug and formulation composition may ultimately affect clinical utility.

Detection And Quantification Of Poliovirus Infection Using Ftir Spectroscopy And Cell Culture, Felipe T. Lee-Montiel, Kelly A. Reynolds, Mark R. Riley

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Background: In a globalized word, prevention of infectious diseases is a major challenge. Rapid detection of viable virus particles in water and other environmental samples is essential to public health risk assessment, homeland security and environmental protection. Current virus detection methods, especially assessing viral infectivity, are complex and time-consuming, making point-of-care detection a challenge. Faster, more sensitive, highly specific methods are needed to quantify potentially hazardous viral pathogens and to determine if suspected materials contain viable viral particles. Fourier transform infrared (FTIR) spectroscopy combined with cellular-based sensing, may offer a precise way to detect specific viruses. This approach utilizes infrared …

Energy Efficiency Technologies For Buildings: Potential For Energy, Cost, And Carbon Emission Savings, Bukola S. Jimoh

CMC Senior Theses

Buildings are a significant energy consumer and are responsible for an increasingly large percent of worldwide greenhouse gas emissions, currently between 30 and 40 percent. Energy efficiency presents unique opportunities for building owners to reduce their environmental footprint and add value through cost savings, tax deductions, and increased market value. An analysis of 183 samples of efficiency measures in seven technology categories found that 74% of efficiency investments had a positive net present value. Building automation system and chiller plant improvements had the highest mean energy and carbon dioxide savings per square foot. Additionally, building automation systems had, on average …

Decision Aid Models For Resource Sharing Strategies During Global Influenza Pandemics, Alfredo Santana Reynoso

USF Tampa Graduate Theses and Dissertations

Pandemic influenza outbreaks have historically entailed significant societal and economic disruptions. Today, our quality of life is threatened by our inadequate preparedness for the imminent pandemic. The key challenges we are facing stem from a significant uncertainty in virus epidemiology, limited response resources, inadequate international collaboration, and the lack of appropriate science-based decision support tools. The existing literature falls short of comprehensive models for global pandemic spread and mitigation which incorporate the heterogeneity of the world regions and realistic travel networks. In addition, there exist virtually no studies which quantify the impact of resource sharing strategies among multiple countries. This …

Asphaltene As Light Harvesting Material In Dye Sensitized Solar Cell, Rajab Emhemed Abujnah

Open Access Theses & Dissertations

This Dissertation characterizes the first asphaltene DSSC (dye-sensitized solar cell), in which light to electricity conversion efficiency has improved from 0 % to 1.8 %. Four natural organic asphaltene factions, namely, un-fractionated asphaltene, Fr. 1 Asphaltene, Fr.2 asphaltene, Fr.3 and Fr.4 were extracted and tested for various solar cell parameters of TiO2 based dye-sensitized solar cell DSSC. DSSCs were fabricated using the four individual asphaltene dyes. The photovoltaic performance of the cells was examined by determining the various parameters such as open-circuit voltage, short-circuit current, fill factor, and series resistance. The overall energy conversion efficiency was also measured to correlate …

The Response Of Pseudomonas Aeruginosa Biofilm To The Presence Of A Glass Polyalkenoate Cement Formulated From A Silver Containing Glass, A. Coughlan, M. P. Ryan, N. M. Cummins, Mark R. Towler

Chemical and Biochemical Engineering Faculty Research & Creative Works

No abstract provided.

Environmental Factors Impacting Coastal Beach Health, Kevin Michael Chenier

LSU Master's Theses

The objectives of this research were to 1) determine the variability of fecal-indicator bacteria in Gulf of Mexico marine waters, 2) determine the effect of environmental variables on enterococci concentrations, 3) determine if beach sand at Holly Beach, LA was capable of acting as a reservoir for fecal-indicator bacteria, and 4) determine if a model could be developed to reduce the time it takes to issue a beach swim advisory. Fecal-indicator bacteria concentrations varied from year to year in historical data collected from a Gulf Coast beach. Afternoon fecal-indicator bacteria concentrations were consistently lower than morning concentrations, demonstrating daily variability. …

Environmental Health In The Latin American And Caribbean Region: Use Of Water Storage Containers, Water Quality, And Community Perception, Erlande Omisca

USF Tampa Graduate Theses and Dissertations

Water quality and availability are important issues in many developing countries where portions of populations still lack access to potable water. Throughout the English-speaking Caribbean and parts of Latin America, households and businesses invest in water supply systems even when they are connected to and pay for water services from a private or state owned provider. Inconsistent supplies of water from the water companies have led many people to invest in storage tanks which, if operated correctly, can provide water throughout the day even when the supply from the main is low or zero. While these individual systems help to …

Surface Functionalization And Analysis Thereof For An Ovarian Cancer Diagnostic Biosensor, Asad Ali Ahmad

USF Tampa Graduate Theses and Dissertations

Ovarian cancer is the fifth leading cause of cancer death among women in United States and has an alarming 1.4% (1 in 71) lifetime risk. The lack of overt symptoms and the absence of a reliable screening test to detect ovarian cancer result in over 70% of women being diagnosed after the disease has spread beyond the ovary resulting in a poor prognosis. A key characteristic of ovarian cancer is the ability of tumor cells to evade apoptosis, or programmed cell death contributing to the limitless replicative potential, which is a hallmark of all carcinogenesis. There is conclusive evidence that …

Flight Physician - December, 2010, Civil Aviation Medical Association

Browse all Civil Aviation Medical Association Newsletters

A sixteen page newsletter of the Civil Aviation Medical Association. The newsletter provided news about civil aviation medicine and information related to the organization.

Plasma Mediated Molecular Delivery, Richard J. Connolly

USF Tampa Graduate Theses and Dissertations

Non-viral delivery of plasmid DNA has traditionally relied upon physical forces applied directly to target tissues. These physical methods typically involve contact between an applicator and the target tissue and often cause transient patient discomfort. To overcome the contact-dependent limitations of such delivery methodologies, an atmospheric direct current plasma source was developed to deposit ionized gas molecules onto localized treatment sites. The deposition of charged species onto a treatment site can lead to the establishment of an electric field with strengths similar to those used for traditional electroporation. In vitro experiments proved that this technology could transiently permeabilize cell membranes …

Handling External Events Efficiently In Gillespie's Stochastic Simulation Algorithm, Brad Geltz

Theses and Dissertations

Gillespie's Stochastic Simulation Algorithm (SSA) provides an elegant simulation approach for simulating models composed of coupled chemical reactions. Although this approach can be used to describe a wide variety biological, chemical, and ecological systems, often systems have external behaviors that are difficult or impossible to characterize using chemical reactions alone. This work extends the applicability of the SSA by adding mechanisms for the inclusion of external events and external triggers. We define events as changes that occur in the system at a specified time while triggers are defined as changes that occur to the system when a particular condition is …

Development Of Sustainability Indicators For Products And Processes, Sundeep Bodapati

Mechanical & Aerospace Engineering Theses & Dissertations

Significant increase in industrialization is a leading cause for the depletion of global resources and changes in the earth's atmosphere due to global warming. There has been a major concern for the manufacturing industry to deal with the above problems and this had led the researchers to focus on the area of sustainability. Sustainable development aims for the future, which is characterized by the interaction of the economic, environment and society. To enable the manufacturing companies to measure sustainability performance, developing the metrics for sustainability is critical. The purpose of this thesis is to review the indicators published in the …