Digital Commons Network^™

Long-Range Pollution Transport During The Milagro-2006 Campaign: A Case Study Of A Major Mexico City Outflow Event Using Free-Floating Altitude-Controlled Balloons, P. B. Voss, R. A. Zaveri, F. M. Flocke, H. Mao, T. P. Hartley, P. Deamicis, I. Deonandan, G. Contreras-Jiménez, O. Martínez-Antonio, M. Figueroa Estrada, D. Greenberg, T. L. Campos, A. J. Weinheimer, D. J. Knapp, D. D. Montzka, J. D. Crounse, P. O. Wennberg, E. Apel, S. Madronich, B. De Foy

Engineering: Faculty Publications

One of the major objectives of the Megacities Initiative: Local And Global Research Observations (MILAGRO-2006) campaign was to investigate the long-range transport of polluted Mexico City Metropolitan Area (MCMA) outflow and determine its downwind impacts on air quality and climate. Six research aircraft, including the National Center for Atmospheric Research (NCAR) C-130, made extensive chemical, aerosol, and radiation measurements above MCMA and more than 1000 km downwind in order to characterize the evolution of the outflow as it aged and dispersed over the Mesa Alta, Sierra Madre Oriental, Coastal Plain, and Gulf of Mexico. As part of this effort, free-floating …

Rapid Cell Extraction In Aqueous Two-Phase Microdroplet Systems, Kalpana Vijayakumar, Shelly Gulati, Andrew J. De Mello, Joshua B. Edel

All Faculty Articles - School of Engineering and Computer Science

Distinguishing specific cells is an essential technique in cell research and clinical diagnostics. We report a novel method to passively isolate and extract cells in a microfluidic device. We utilise a droplet-based microfluidic system to generate an aqueous two phase system in which aqueous droplets consist of two phases in the form of a double emulsion. Specifically, we generate PEG droplets that completely encapsulate DEX droplets within a microfluidic channel. Target cells can be introduced directly into the droplets and driven to partition to the more favourable phase, whilst still being contained within the aqueous droplet. Human T lymphoma cells, …

A Monocular Marker-Free Gait Measurement System, Jane Courtney, Annraoi M. De Paor

Articles

This paper presents a new, user-friendly, portable motion capture and gait analysis system for capturing and analyzing human gait, designed as a telemedicine tool to monitor remotely the progress of patients through treatment. The system requires minimal user input and simple single-camera filming (which can be acquired from a basic webcam) making it very accessible to nontechnical, nonclinical personnel. This system can allow gait studies to acquire a much larger data set and allow trained gait analysts to focus their skills on the interpretation phase of gait analysis. The design uses a novel motion capture method derived from spatiotemporal segmentation …

Image Processing Algorithms For Diagnostic Analysis Of Microcirculation, Sumeyra Ummuhan Demir

Theses and Dissertations

Microcirculation has become a key factor for the study and assessment of tissue perfusion and oxygenation. Detection and assessment of the microvasculature using videomicroscopy from the oral mucosa provides a metric on the density of blood vessels in each single frame. Information pertaining to the density of these microvessels within a field of view can be used to quantitatively monitor and assess the changes occurring in tissue oxygenation and perfusion over time. Automated analysis of this information can be used for real-time diagnostic and therapeutic planning of a number of clinical applications including resuscitation. The objective of this study is …

Thin Film Microfluidic And Nanofluidic Devices, Mark Noble Hamblin

Theses and Dissertations

Lab-on-a-chip devices, also known as micro total analysis systems (μTAS), are implementations of chemical analysis systems on microchips. These systems can be fabricated using standard thin film processing techniques. Microfluidic and nanofluidic channels are fabricated in this work through sacrificial etching. Microchannels are fabricated utilizing cores made from AZ3330 and SU8 photoresist. Multi-channel electroosmotic (EO) pumps are evaluated and the accompanying channel zeta potentials are calculated. Capillary flow is studied as an effective filling mechanism for nanochannels. Experimental departure from the Washburn model is considered, where capillary flow rates lie within 10% to 70% of theoretical values. Nanochannels are fabricated …

Arterial Waveform Measurement Using A Piezoelectric Sensor, Ruizhi Zhang

Theses and Dissertations

This study aims to develop a new method to monitor peripheral arterial pulse using a PVDF piezoelectric sensor. After comparing different locations of sensor placement, a specific sensor wrap for the finger was developed. Its composition, size, and location make it inexpensive and very convenient to use. In order to monitor the effectiveness of the sensor at producing a reliable pulse waveform, a monitoring system, including the PZT sensor, ECG, pulse-oximeter, respiratory sensor, and accelerometer was setup. Signal analysis from the system helped discover that the PZT waveform is relative to the 1st derivative of the artery pressure wave. Also, …

Development Of Pyrf-Based Genetic System For Targeted Gene Deletion In Clostridium Thermocellum And Creation Of A Pta Mutant, Shital A. Tripathi, Daniel G. Olson, D. Aaron Argyros, Bethany B. Miller, Trisha F. Barrett, Daniel M. Murphy, Jesse D. Mccool, Anne K. Warner, Vineet B. Rajgarhia, Lee R. Lynd, David A. Hogsett, Nicky C. Caiazza

Dartmouth Scholarship

We report development of a genetic system for making targeted gene knockouts in Clostridium thermocellum, a thermophilic anaerobic bacterium that rapidly solubilizes cellulose. A toxic uracil analog, 5-fluoroorotic acid (5-FOA), was used to select for deletion of the pyrF gene. The ΔpyrF strain is a uracil auxotroph that could be restored to a prototroph via ectopic expression of pyrF from a plasmid, providing a positive genetic selection. Furthermore, 5-FOA was used to select against plasmid-expressed pyrF, creating a negative selection for plasmid loss. This technology was used to delete a gene involved in organic acid production, namely pta, which encodes …

Real Time 3-D Tracking Of The High Dose Rate Radiation Source Using A Flat Panel Detector, Aditya Bondal

Theses and Dissertations

A number of QA procedures have been developed for Breast Brachytherapy treatments, yet none guarantee accurate dose delivery or allow conformation of the actual source position leading to errors sometimes going unnoticed. The objective of this study is to track the exact path the HDR source would follow in real time. The exit radiation of the HDR source was used to image a well defined matrix of markers. The images were acquired using FPD and were processed to obtain projection coordinates while an x-ray calibration image was processed to obtain marker coordinates. Each marker along with its projection represents a …

Corrosion Dynamics Of Cobalt-Chromium Alloy F-75 Powder, Tedman Tong

Master's Theses

The increasing usage of metal-on-metal joint replacements consisting of a cobalt-chromium-molybdenum alloy requires increasing concern regarding the inevitable generation of metallic wear debris. Patients with these joint replacements exhibit elevated concentrations of cobalt and chromium ions within their serum, blood and urine. The presence of these metal ions suggests the potential for bodily damage and indicates corrosive processes are acting upon wear debris.

To understand the behavior of these corrosive processes, powders of cobalt-chromium-molybdenum alloy F-75 were studied. Four powder sizes (44, 74, 105, and 420 µm diameter) were subjected to Hank’s Balanced Salt Solution (HBSS) for a 42 day …

Cantor's Paradise Regained: Constructive Mathematics From Brouwer To Kolmogorov To Gelfond, Vladik Kreinovich

Departmental Technical Reports (CS)

Constructive mathematics, mathematics in which the existence of an object means that that we can actually construct this object, started as a heavily restricted version of mathematics, a version in which many commonly used mathematical techniques (like the Law of Excluded Middle) were forbidden to maintain constructivity. Eventually, it turned out that not only constructive mathematics is not a weakened version of the classical one -- as it was originally perceived -- but that, vice versa, classical mathematics can be viewed as a particular (thus, weaker) case of the constructive one. Crucial results in this direction were obtained by M. …

Compaction Characteristics Of Municipal Solid Waste, James L. Hanson, Nazli Yesiller, Shawna A. Von Stockhausen, Wilson W. Wong

Civil and Environmental Engineering

Compaction characteristics of municipal solid waste (MSW) were determined in the laboratory and in the field as a function of moisture content, compactive effort, and seasonal effects. Laboratory tests were conducted on manufactured wastes using modified and 4X modified efforts. Field tests were conducted at a MSW landfill in Michigan on incoming wastes without modifications to size, shape, or composition, using typical operational compaction equipment and procedures. Field tests generally included higher efforts and resulted in higher unit weights at higher water contents than the laboratory tests. Moisture addition to wastes in the field was more effective in winter than …

Intricacies Of Professional Certifications For Quality Management (Qm), Edmund R. Martelli

Masters Theses & Specialist Projects

The modern manufacturing marketplace is extremely complex and constantly changing. Career professionals often seek job placement, career advancement, and continuing education through professional certifications. Professional certifications in the United States (U.S.) have been evolving since 1911. In the 1950s, engineering groups established professional certifications and licenses. In the 1980s, administration of accreditation of certification programs proliferated, and by the 1990s, many government and professional organizations sought to standardize procedures for the industry. Currently, professional certifications are available for virtually every profession and issued by multitudes of organizations.

Quality management (QM) encompasses an extensive combination ofprofessional abilities. Providers of professional certifications …

Newsletter 42 Issue 2 - Summer 2010 - Nuts And Bolts, Tractor Museum

Friends of the Larsen Tractor Museum

No abstract provided.

52nd Rocky Mountain Conference On Analytical Chemistry

Rocky Mountain Conference on Magnetic Resonance

Final program, abstracts, and information about the 52nd annual meeting of the Rocky Mountain Conference on Analytical Chemistry, co-endorsed by the Colorado Section of the American Chemical Society and the Society for Applied Spectroscopy. Held in Snowmass, Colorado, August 1-5, 2010.

St. Jude Medical: Enhanced Mics (Emics), Devanshi Shah

Master's Theses

Heart disease is one of the most prevalent diseases in the world. The survival chances for patients with ventricular fibrillation/ventricular tachycardia reduces significantly as time passes without treatment and even after getting timely treatment recurring episode are common. These patients can benefit from an Implantable Cardioverter Defibrillator (ICD) which can monitor heart rhythm and provide immediate treatment. Due to the ever changing physical conditions and disease progression, the ICD needs to collect diagnostic data as well as support programming by the physician. The ICD uses inductive telemetry and radio-frequency telemetry for the communication with the external devices such as a …

Clinical And Mechanical Evaluation Of A New All-Ceramic Restorative Material Turkom-Cera., Bandar Mohammed Abdullah Al-Makramani

Student Works (2010-2019)

The mechanical properties and clinical performance of Turkom-Cera system were evaluated in this study. The mechanical properties evaluated were; flexural strength, microhardness, shear bond strength, fracture resistance and marginal integrity. The biaxial flexural strength and hardness of Turkom-Cera compared to two other allceramic systems (In-Ceram and Vitadur-N) were investigated. The Turkom-Cera exhibited significantly higher flexural strength (506.8 MPa) than In-Ceram (347.4 MPa) and Vitadur-N (128.7 MPa) ceramic materials. However, In-Ceram core has significantly higher hardness (1116.2 VHN) than Turkom-Cera (1002.1 VHN) and Vitadur-N (812.8 VHN) all-ceramic materials. In order to find the optimal choice of luting cement and surface treatment …

Global Competence: Determination Of Its Importance For Engineers Working In A Global Environment, Gregg M. Warnick

Department of Educational Administration: Dissertations, Theses, and Student Research

We live and work in a world that is even more interconnected and interdependent than ever before. Engineers must now not only develop technical engineering competence, but must also develop additional skills and competencies including global competence to obtain success within a global engineering environment.

The purpose of this study was to determine whether multinational companies considered global competence an important skill in mechanical engineering graduates when making hiring decisions. The study was an exploratory study that utilized an extensive literature review to identify eight global competencies for engineering success within a global environment and also included a survey instrument …

Fracture Of Bone Using Microindentation, Séverine Vennin

Department of Engineering Mechanics: Dissertations, Theses, and Student Research

Osteoporosis is a current disease which is especially of concern to post-menopausal women. It is characterized by a decrease of bone density and an increase in the risk of fracture. Interest in the fracture mechanisms with respect to the underlying biological structure of the bone is of great interest to researchers in this field. In this thesis, a new method based on microindentation on rat femurs was developed to determine the relation between the viscoelastic and the fracture properties of bone. The main goal is to measure the viscoelastic properties by using a dynamic mechanical analysis indentation method and then …

Managing Large Data Sets Using Support Vector Machines, Ranjini Srinivas

School of Computing: Dissertations, Theses, and Student Research

Hundreds of Terabytes of CMS (Compact Muon Solenoid) data are being accumulated for storage day by day at the University of Nebraska-Lincoln, which is one of the eight US CMS Tier-2 sites. Managing this data includes retaining useful CMS data sets and clearing storage space for newly arriving data by deleting less useful data sets. This is an important task that is currently being done manually and it requires a large amount of time. The overall objective of this study was to develop a methodology to help identify the data sets to be deleted when there is a requirement for …

In Vitro Analysis Of Auto-Loom Woven Test Patches For Hernia Repair, Bryant Mersereau

All Theses

Hernias are defects in the layers of the abdominal wall that can cause discomfort or pain and lead to serious health problems if left untreated. A significant portion of the world's population is afflicted by hernia formation, and the cost of treating those affected is in the billions of dollars in the US alone. The current best practices for repairing hernias involve the surgical implantation of polymeric meshes over and around the defect site. The mesh, acting as a synthetic replacement for the damaged abdominal wall layers, provides a barrier to further visceral protrusions through the defect, a support framework …

Mitochondrial Free [Ca2+] Increases During Atp/Adp Antiport And Adp Phosphorylation: Exploration Of Mechanisms, Johan Haumann, Ranjan K. Dash, David F. Stowe, Age D. Boelens, Daniel A. Beard, Amadou K.S. Camara

Biomedical Engineering Faculty Research and Publications

ADP influx and ADP phosphorylation may alter mitochondrial free [Ca²⁺] ([Ca²⁺]_m) and consequently mitochondrial bioenergetics by several postulated mechanisms. We tested how [Ca²⁺]_m is affected by H₂PO₄⁻ (P_i), Mg²⁺, calcium uniporter activity, matrix volume changes, and the bioenergetic state. We measured [Ca²⁺]_m, membrane potential, redox state, matrix volume, pH_m, and O₂ consumption in guinea pig heart mitochondria with or without ruthenium red, carboxyatractyloside, or oligomycin, and at several levels of Mg²⁺ and P_i. …

Parametric Instabilities For Vibratory Energy Harvesting Under Harmonic, Time-Varying Frequency, And Random Excitations, Christopher Stabler

All Theses

This effort investigates and evaluates the prospect of using parametric instabilities for vibratory energy harvesting. To that end, we consider a parametrically-excited piezoelectric cantilever beam and study its performance as an energy harvester under i) fixed-frequency harmonic excitations, ii) time-varying frequency excitations, and iii) band-limited Gaussian noise. In the case of fixed-frequency excitations, we use the Method of Multiple Scales to obtain approximate analytical expressions for the steady-state response amplitude and instantaneous output power in the vicinity of the first principle parametric resonance. We show that the electromechanical coupling and load resistance play an important role in determining the output …

Structural Bioinformatics Based Method For Predicting The Initial Adsorbed Protein Orientation On A Surface, Aby Thyparambil

All Theses

In any molecular simulation of protein-surface interaction, the selection of the initial orientation with which the protein would interact with the surface must be first made and is found to be critical in the determination of the bioactive state of the adsorbed protein. While various molecular simulation methods have been developed to identify the preferred orientation, these methods are generally computationally expensive and time consuming, especially for large molecules thereby motivating the current study.
The computational implementation for identifying a preferred orientation was done in MATLAB¨ and directly addresses the current research problem by assuming the protein to be rigid …

Infrared Thermography And Ultrasonic Inspection Of Adhesive Bonded Structures, Overview And Validity, Suhas Vedula

All Theses

The work focuses on establishing a relationship between two different techniques of Nondestructive testing, namely Infrared Thermography and Ultrasonic Inspection. The integration of the two testing techniques is done with an expectation to achieve accurate characterization and determination of internal defects in adhesive bonded structures.
Experiments are done on sandwich samples with defects using various ultrasonic testing methods like pulse-echo and immersion techniques. The results thus obtained are documented and location of the defects is marked. The same samples are then tested using Infrared thermography to visualize the defects.
The results obtained by both the methods are compared to check …

Process Development For The Fractionation And Isolation Of Corn Fiber Hemicellulose, Justin Montanti

All Theses

Corn fiber is a co-product of the corn wet-milling process that holds potential to become a value-added product. A process was developed to fractionate and isolate the hemicellulose B component of corn fiber generated by corn wet milling. The process consisted of pretreatment by soaking in aqueous ammonia (SAA) of starch-free corn fiber followed by enzymatic cellulose hydrolysis, during which the hemicellulose B was solubilized by cleavage into xylo-oligosaccharides. The hemicellulose A and B fractions were separated by adjustment of pH, and the hemicellulose B recovered by precipitation with ethanol.
The pretreatment step resulted in a high retention of major …

Sensor Based Nitrogen Management For Cotton Production In Coastal Plain Soils, Wesley Porter

All Theses

The main objective of this four year study was to develop, refine, and employ sensor-based algorithims to determine the mid season nitrogen requirements for production of irrigated and dryland cotton (Gossypium hirsutum L.) in Coastal Plains soils. The secondary objective of the project was to develop and test equipment for variable rate application of nitrogen to commerical cotton fields utilizing the developed algorithim. Two different production fields at Clemson's Edisto Research and Education Center near Blackville, SC were used. One field, equiped with an overhead irrigation system, was used during the 2007 and 2010 production seasons to develop the algorithm …

The Effects Of Physical Factors On The Adsorption Of Synthetic Organic Compounds By Activated Carbons And Activated Carbon Fibers, Hatice Kose

All Theses

Activated carbons (ACs) and activated carbon fibers (ACFs) have been extensively used for the removal of synthetic organic compounds (SOCs) that have been found to be toxic, carcinogenic, mutagenic or teratogenic. Adsorption of these compounds on ACs and ACFs are controlled by both physical factors and chemical interactions, which depend on the characteristics of the adsorbent (surface area, pore size distribution (PSD), and surface chemistry), the nature of the adsorbate (molecular weight and size, functional groups, polarity, solubility), and the condition of the background solution (pH, temperature, presence of competitive solutes, ionic strength). Since there are several mechanisms that can …

Remote Usability Testing - A New Approach Facilitated By Virtual Worlds, Kapil Chalil Madathil

All Theses

Synchronous remote usability testing, involves a facilitator conducting a usability test in real time, interacting with a participant who is remote. This study proposes a new methodology for conducting these studies using a three-dimensional virtual world, Wonderland, and compares it with two other commonly used synchronous usability test methods: the traditional lab approach and WebEx, a web-based conferencing and screen sharing approach.
The study involved 48 participants in total, 36 test subjects and 12 test facilitators. These 36 were equally divided among the three environments with the 12 test facilitators being paired with one participant in each of the environments. …

Evaluating Transit Systems In A University Environment The Clemson University Case Study, Katerina Bartman

All Theses

With the economy in a slow recovery, enrollment in higher education is
increasing. This means that universities across the country must accommodate these new students, their vehicles, and local transportation needs. Campus setting and ambiance is a treasured quality on a university campus resulting in the approval of additional surface lots and parking garages being difficult or restricted. To combat the increased number of single occupancy vehicles, universities are developing and encouraging the use of multimodal transportation by providing pedestrian, bicycle, and public transportation facilities along with providing users with the information necessary to make the optimal modal choice (Boyles, …

Integration Of Microfluidics With Surface Plasmon Resonance, Scott B. Fratzke

Master's Theses

This thesis successfully integrates laminate microfluidic devices with an analytic Surface Plasmon Resonance (SPR) instrument. Integration was accomplished at low-cost using materials such as polydimethylsiloxane (PDMS), Poly(methyl methacrylate) (PMMA), Tygon tubing, and a 3-way stopcock. The main components of this thesis are the design and fabrication of the low-cost, in-house fluidics that can integrate with upstream microfluidics and the validation of the in-house fluidics using the Biosensing Instruments BI-2000 SPR instrument. The low-cost fluidics was designed and fabricated “in-house” using a novel investment casting technique that required the use of laser cutting technology to make a master cast, and candle …