Digital Commons Network^™

Probing Dna Structural Heterogeneity By Identifying Conformational Subensembles Of A Bicovalently Bound Cyanine Dye, Matthew S. Barclay, Azhad U. Chowdhury, Austin Biaggne, Jonathan S. Huff, Nicholas D. Wright, Paul H. Davis, Lan Li, William B. Knowlton, Bernard Yurke, Ryan D. Pensack, Daniel B. Turner

Materials Science and Engineering Faculty Publications and Presentations

DNA is a re-configurable, biological information-storage unit, and much remains to be learned about its heterogeneous structural dynamics. For example, while it is known that molecular dyes templated onto DNA exhibit increased photostability, the mechanism by which the structural dynamics of DNA affect the dye photophysics remains unknown. Here, we use femtosecond, two-dimensional electronic spectroscopy measurements of a cyanine dye, Cy5, to probe local conformations in samples of single-stranded DNA (ssDNA–Cy5), double-stranded DNA (dsDNA–Cy5), and Holliday junction DNA (HJ–DNA–Cy5). A line shape analysis of the 2D spectra reveals a strong excitation–emission correlation present in only the dsDNA–Cy5 complex, which is …

Co-Culture Models Of Endothelial Cells, Macrophages, And Vascular Smooth Muscle Cells For The Study Of The Natural History Of Atherosclerosis, Martin Liu, Saurabhi Samant, Charu Hasini Vasa, Ryan M. Pedrigi, Usama Oguz, Sangjin Ryu, Timothy Wei, Daniel R. Anderson, Devandra K. Agrawal, Yiannis Chatzizisis

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Background

This work aims to present a fast, affordable, and reproducible three-cell co-culture system that could represent the different cellular mechanisms of atherosclerosis, extending from atherogenesis to pathological intimal thickening.

Methods and results

We built four culture models: (i) Culture model #1 (representing normal arterial intima), where human coronary artery endothelial cells were added on top of Matrigel-coated collagen type I matrix, (ii) Culture model #2 (representing atherogenesis), which demonstrated the subendothelial accumulation and oxidative modification of low-density lipoproteins (LDL), (iii) Culture model #3 (representing intimal xanthomas), which demonstrated the monocyte adhesion to the endothelial cell monolayer, transmigration into the …

Utilizing Drones To Streamline Wildlife Hazard Management Efforts By Airport Operators, Flavio A. C. Mendonca Ph.D., Ryan Wallace, Jose Cabrera, Cole Mcnall

National Training Aircraft Symposium (NTAS)

Airports operating under the Code of Federal Regulations Part 139 should conduct a wildlife hazard assessment (WHA) when some wildlife-strike events have occurred at or near the airport. The WHA must be conducted by a Qualified Airport Wildlife Biologist (QAWB). The required elements in a WHA include the identification of the wildlife species observed and their numbers, and the location of features on and near the airport that could attract wildlife. Service learning and research projects enable undergraduate students to develop transferable skills that are highly valued by the aviation industry, including critical thinking, self-confidence, the ability to integrate theory …

Improved Feedback Mechanisms Of The Hydraulics Sandbox Simulator, Karen J. Johnson, Martin Hebel

National Training Aircraft Symposium (NTAS)

The proposed presentation will include the following project and results from implementation in an aircraft hydraulic systems course.

In 2020 we developed a digital hydraulics system schematic sandbox for use in an FAA Part 147 airframe maintenance technician training program (See Figure 1). The sandbox allows students to drag-drop-connect components used in systems such as those found in aircraft. It allows them to ‘test-connect’ the components to get feedback on correct and incorrect ‘construction’. As shown in Figure 1, light green ‘hoses’ are correct supply connections, dark green are correct return connections and red are incorrect connections per established rules …

Static And Dynamic Analysis Of A Bistable Frequency Up-Converter Piezoelectric Energy Harvester, Mohammad Atmeh, Alwathiqbellah Ibrahim, Abdallah Ramini

Mechanical Engineering Faculty Publications and Presentations

Using energy harvesting to convert ambient vibrations efficiently to electrical energy has become a worthy concept in recent years. Nevertheless, the low frequencies of the ambient vibrations cannot be effectively converted to power using traditional harvesters. Therefore, a frequency up-conversion harvester is presented to convert the low-frequency vibrations to high-frequency vibrations utilizing magnetic coupling. The presented harvester consists of a low-frequency beam (LFB) and a high-frequency beam (HFB) with identical tip magnets facing each other at the same polarity. The HFB, fully covered by a piezoelectric strip, is utilized for voltage generation. The dynamic behavior of the system and the …

Artificial Intelligence For Automated Detection Of Large Mammals Creates Path To Upscale Drone Surveys, Javier Lenzi, Andrew Barnas, Abdelrahman A. Elsaid, Travis Desell, Robert F. Rockwell, Susan N. Ellis-Felege

Biology Faculty Publications

Imagery from drones is becoming common in wildlife research and management, but processing data efficiently remains a challenge. We developed a methodology for training a convolutional neural network model on large-scale mosaic imagery to detect and count caribou (Rangifer tarandus), compare model performance with an experienced observer and a group of naïve observers, and discuss the use of aerial imagery and automated methods for large mammal surveys. Combining images taken at 75 m and 120 m above ground level, a faster region-based convolutional neural network (Faster-RCNN) model was trained in using annotated imagery with the labels: “adult …

Structural Integrity Analysis Of The Rig Mast Following Repair Of Two Diagonal Braces In The Upper Mast Section, Rendhatya Padmodwiputra, Johny Wahyuadi Soedarsono, Mirza Mahendra, Bambang Eka Satria, Chandra Arif, Eka Juni Suarthana

Journal of Materials Exploration and Findings

During a move to the new drilling location, two braces of the upper mast section on a 550 HP oil and gas rig was damaged. When damage is discovered, the rig operating company should conduct a risk analysis related to the damage using the standard used to determine rejection criteria and follow-up repairs. The strength of the rig mast is calculated using Finite Element Analysis (FEA) with initial conditions (before damage occurs) where all braces are installed and extreme conditions where neither brace is repaired. The calculation results are then used to assess the risk of reducing the rig mast …

Temperature, Pressure, And Adsorption Dependent Redox Potentials: Iii. Processes Of Co Conversion To Value-Added Compounds, Siyuan Fang, Yun Hang Hu

Michigan Tech Publications, Part 1

Carbon monoxide (CO) is a primary air pollutant and a poisonous species for human beings, animals, and some catalytic reactions. Meanwhile, CO is also a versatile feedstock in the chemical industry to produce high‐value chemicals and clean fuels, which has stimulated extensive research interests in exploiting efficient CO conversion processes. Redox potential is a key thermodynamic quantity in these processes whereas only standard reduction potentials at 25°Cand 1 atm are currently available. Herein, it is the first time to report the effects of temperature (0–1000°C), pressure (1–100 atm), and adsorption on the redox potentials of 18 CO conversion reactions to …

Enhancing Sustainable Process Designs Through Strategic Material Utilization And Waste Minimization Approaches, John David Chea

Theses and Dissertations

Sustainability is a growing concern as resources are continually depleted for various applications without adequate renewal plans. The resulting impacts on the ecosystem, health, and resource circularity are often overlooked. This research analyzes improvement opportunities at each major stage in a product's life cycle. Raw material acquisition, product synthesis, process waste management, and the fate of a material in the end-of-life phases were examined. The viability of utilizing renewable resources has been demonstrated in this work by extracting bio-based chemicals from underutilized renewable resources at a commercial scale and transforming the extracted resources into polymeric materials. The optimization of raw …

Greywater Treatment Using Integrated Sand Filtration With Ultrafiltration For Reuse, Aya El-Wattar

Theses and Dissertations

Membrane flux was observed and the two membrane sizes that achieved stable flux were chosen to be used in the later stages of the study. The chosen membranes were of 300 kDa and 400 kDa Molecular Weight Cutoff (MWCO).

The third phase of the study involved running the full system on synthetic greywater then on real greywater. The parameters investigated were COD, TSS, Turbidity and BOD₅. Total COD removal after sand filtration, 300kDa UF and 400 kDa UF was 65, 66 and 73.4% respectively. Total TSS removal after sand filtration, 300kDa UF and 400 kDa UF was 93.1, …

Using Innovative Copper‑Loaded Activated Alumina (Cu/Aa) As The Catalyst For Catalytic Wet Peroxidation (Cwpo) Of Catechol, Vijayalakshmi Gosu, Archana Dhakar, Tian C. Zhang, Rao Y. Surampalli, Verraboina Ubbaramaiah

Department of Civil and Environmental Engineering: Faculty Publications

In this study, copper-loaded activated alumina (Cu/AA) was synthesized and used for the CWPO of catechol (a representative refractory organic pollutant). Various characterization techniques were deployed to characterize the catalysts, e.g., activated alumina (AA), as well as pristine and spent 1% Cu/AA. The innovative 1% Cu/AA catalyst exhibited good thermal stability up to 1173 K with a marginal weight loss of 13%. The Cu species were well dispersed on the activated alumina framework with no significant cluster formation. Typically, the average copper particle size of 5 nm was dispersed on the AA framework. Catechol removal was observed to be 92% …

Poly(Pro-Curcumin) Materials Exhibit Dual Release Rates And Prolonged Antioxidant Activity As Thin Films And Self-Assembled Particles, Ruiwen Chen, Jessica L. Funnell, Geraldine B. Quinones, Marvin Bentley, Jeffrey R. Capadona, Ryan J. Gilbert, Edmund F. Palermo

Chemical and Biochemical Engineering Faculty Research & Creative Works

Curcumin is a natural polyphenol that exhibits remarkable antioxidant and anti-inflammatory activities; however, its clinical application is limited in part by its physiological instability. Here, we report the synthesis of curcumin-derived polyesters that release curcumin upon hydrolytic degradation to improve curcumin stability and solubility in physiological conditions. Curcumin was incorporated in the polymer backbone by a one-pot condensation polymerization in the presence of sebacoyl chloride and polyethylene glycol (PEG, Mn = 1 kDa). The thermal and mechanical properties, surface wettability, self-assembly behavior, and drug-release kinetics all depend sensitively on the mole percentage of curcumin incorporated in these statistical copolymers. Curcumin …

New Types Of Topologies And Neutrosophic Topologies, Florentin Smarandache

Neutrosophic Systems with Applications

In this paper, we recall the six new types of topologies that we introduced in the last years (2019-2022), such as: Refined Neutrosophic Topology, Refined Neutrosophic Crisp Topology, NeutroTopology, AntiTopology, SuperHyperTopology, and Neutrosophic SuperHyperTopology.

Engineering At-Home Dilution And Filtration Methods To Enable Paper-Based Colorimetric Biosensing In Human Blood With Cell-Free Protein Synthesis, Tyler J. Free, Ryan W. Tucker, Katelyn M. Simonson, Sydney A. Smith, Caleb M. Lindgren, William G. Pitt, Bradley C. Bundy

Faculty Publications

Diagnostic blood tests can guide the administration of healthcare to save and improve lives. Most clinical biosensing blood tests require a trained technician and specialized equipment to process samples and interpret results, which greatly limits test accessibility. Colorimetric paper-based diagnostics have an equipment-free readout, but raw blood obscures a colorimetric response which has motivated diverse efforts to develop blood sample processing techniques. This work uses inexpensive readily-available materials to engineer user-friendly dilution and filtration methods for blood sample collection and processing to enable a proof-of-concept colorimetric biosensor that is responsive to glutamine in 50 µL blood drop samples in less …

Comparison Of In-Vitro 3d Human Embryoids With Current Models For Gastrulation, Jin Park

McKelvey School of Engineering Graduate Student Theses & Dissertations

Gastrulation is an early morphogenetic process that is conserved across most metazoans and lays out the future body plan through the formation and shaping of the three germ layers: endoderm, mesoderm, and ectoderm. Despite its importance, not much is known about the events surrounding human gastrulation that occurs in utero due to ethical and technical limitations on studying human embryos. Therefore, many researchers have devised protocols for creating in vitro models of gastrulation using embryonic stem cells. Initially starting with mouse embryonic stem cells, the field of in vitro embryo models has advanced rapidly, with protocols using human embryonic stem …

Problem-Based Learning Of Heuristic Methods For Decision Problems In Mathematics, Computer Science And Industrial Engineering, Felix Engelhardt, Christina Büsing, Sabrina Schmitz

Practice Papers

In a digitalized world, most processes can be formalised, measured and described mathematically. The use of analytical methods to optimise such models and decisions constitutes operational research (OR), developing new methods for a specific problem and analysing them are part of discrete optimisation (DO). However, there is limited research on OR and application driven DO in higher education. Furthermore, neither is well integrated into engineering education research.

In this work, we present a case study of an interdisciplinary Master’s course on heuristic methods in the context of OR and DO. We discuss to what extent wellestablished approaches from engineering education …

Actividad 2: Proyecto Pb&J, Patrick Young

Tema 1: Estructura y proceso

Los proyectos complejos, como la construcción de un puente, requieren una planificación minuciosa. La gestión de proyectos es la disciplina que consiste en planificar y controlar el trabajo de un equipo para alcanzar un objetivo específico. El éxito de la gestión de proyectos requiere una cuidadosa consideración de los plazos, los presupuestos y los recursos disponibles.

El diagrama de Gantt es una herramienta de gestión de proyectos que muestra las tareas en términos de su tiempo de ejecución, interdependencia y recursos disponibles. Desarrollado por el ingeniero estadounidense Henry Gantt, este diagrama se empleó por primera vez en la construcción naval, …

Actividad 1: ¡Construye Fuerte!, Patrick Young

Tema 1: Estructura y proceso

El hormigón es el material de construcción más común para estructuras grandes. El hormigón se compone de tres materiales:

• Áridos: Arena y grava.
• Cemento: Un pegamento químico que mantiene los agregados unidos con gran fuerza.
• Agua: El agua no solo ayuda a mezclar y verter el hormigón, sino que se une al cemento en una reacción química y se incorpora al hormigón terminado.

Las cubiertas de los puentes deben soportar el peso de todos los automóviles y camiones que transitan por ellos. Las diferentes partes de las cubiertas de los puentes experimentan distintos tipos de fuerzas. La compresión es una …

Actividad 1: Lo De Adela, Patrick Young

Tema 2: Por la ciudad

Si pregunta a algunos de los residentes más antiguos de su barrio cómo era la ciudad hace 30 o 40 años, le dirán que había muchos espacios o campos vacíos Si les pregunta si están contentos con las numerosas viviendas, comercios, carreteras e industrias que existen ahora, es posible que digan que han perdido parte de su comunidad, pero que, al mismo tiempo, han ganado en infraestructuras, carreteras, industrias, puestos de trabajo, valor de la propiedad y expansión de la comunidad.

Implantar más carreteras en una ciudad no es una decisión fácil. Para que el proyecto se lleve a cabo, …

Actividad 2: Ruta Óptima, Patrick Young

Tema 2: Por la ciudad

Los sistemas de navegación de los vehículos utilizan información de la ubicación para ayudar a los conductores a decidir qué ruta tomar. Muchos de los sistemas de navegación alertan a los conductores de peligros en la carretera. Los cierres de carreteras, los accidentes, el clima y otros peligros afectan tanto los tiempos de viaje como las rutas elegidas. La colaboración y el intercambio de información entre la autopista o carretera, los servicios públicos y los sistemas de navegación son necesarios para informar a los conductores.

A Path Planning Framework For Multi-Agent Robotic Systems Based On Multivariate Skew-Normal Distributions, Peter Estephan

Theses, Dissertations and Capstones

This thesis presents a path planning framework for a very-large-scale robotic (VLSR) system in an known obstacle environment, where the time-varying distributions of agents are applied to represent the multi-agent robotic system (MARS). A novel family of the multivariate skew-normal (MVSN) distributions is proposed based on the Bernoulli random field (BRF) referred to as the Bernoulli-random-field based skew-normal (BRF-SN) distribution. The proposed distributions are applied to model the agents’ distributions in an obstacle-deployed environment, where the obstacle effect is represented by a skew function and separated from the no-obstacle agents’ distributions. First, the obstacle layout is represented by a Hilbert …

Special Collections As Muse: The Use Of Rare Books And Archives To Inspire Creative Works, Tracy Grimm, Adriana Harmeyer

Faculty & Staff Scholarship

The unique and varied collections held by archives and special collections within many academic libraries offer fertile ground for the creative endeavors of students, faculty, and professional artists. This chapter explores direct and indirect methods librarians and archivists may engage creators with primary source materials. Academic libraries do not necessarily need to build art-focused collections in order to support the research of creators. More than subject content, successful engagement with creators is developed by means of collaborative relationships with arts faculty, artists, and galleries to reach student creators and introduce concepts of primary source research as a source of inspiration. …

Efficient Cloud-Based Ml-Approach For Safe Smart Cities, Niveshitha Niveshitha

Browse all Theses and Dissertations

Smart cities have emerged to tackle many critical problems that can thwart the overwhelming urbanization process, such as traffic jams, environmental pollution, expensive health care, and increasing energy demand. This Master thesis proposes efficient and high-quality cloud-based machine-learning solutions for efficient and sustainable smart cities environment. Different supervised machine-learning models for air quality predication (AQP) in efficient and sustainable smart cities environment is developed. For that, ML-based techniques are implemented using cloud-based solutions. For example, regression and classification methods are implemented using distributed cloud computing to forecast air execution time and accuracy of the implemented ML solution. These models are …

Contributors To Pathologic Depolarization In Myotonia Congenita, Jessica Hope Myers

Browse all Theses and Dissertations

Myotonia congenita is an inherited skeletal muscle disorder caused by loss-of-function mutation in the CLCN1 gene. This gene encodes the ClC-1 chloride channel, which is almost exclusively expressed in skeletal muscle where it acts to stabilize the resting membrane potential. Loss of this chloride channel leads to skeletal muscle hyperexcitability, resulting in involuntary muscle action potentials (myotonic discharges) seen clinically as muscle stiffness (myotonia). Stiffness affects the limb and facial muscles, though specific muscle involvement can vary between patients. Interestingly, respiratory distress is not part of this disease despite muscles of respiration such as the diaphragm muscle also carrying this …

Friend Or Foe? The Role Of Transforming Growth Factor-Β (Tgfβ) Signaling In Calcineurin Inhibitor-Induced Renal Damage, Adaku Uwe

Browse all Theses and Dissertations

With its incorporation into clinical practice in the early 1980s, the class of pharmacological agents known as calcineurin inhibitors (CNIs) quickly became the cornerstone of immunosuppressive therapy post-organ transplantation. However, its use is limited by irreversible kidney damage in the form of renal fibrosis. The molecular mechanism by which CNIs induce renal fibrosis remains to be better understood, and to date, there are no specific therapeutic strategies to mitigate this damage. This dilemma presents a critical need to explain mechanisms by which CNIs cause renal damage. Kidneys of patients on chronic CNI therapy show increased expression of the proinflammatory cytokine …

Hmm, You Seem Confused! Tracking Interlocutor Confusion For Situated Task-Oriented Hri, Na Li, Robert J. Ross

Articles

Our research seeks to develop a long-lasting and high-quality en- gagement between the user and the social robot, which in turn requires a more sophisticated alignment of the user and the system than is currently commonly available. Close monitoring of inter- locutors’ states, and we argue their confusion state in particular, and adjusting dialogue policies based on this state of confusion is needed for successful joint activity. In this paper, we present an ini- tial study of a human-robot conversation scenarios using a Pepper robot to investigate the confusion states of users. A Wizard-of-Oz (WoZ) HRI experiment is illustrated in …

Simulating The Effect Of Gut Microbiome On Cancer Cell Growth Using A Microfluidic Device, Ekansh Mittal, Grace Cupp, Youngbok (Abraham) Kang

Faculty Publications - Biomedical, Mechanical, and Civil Engineering

The imbalance in the gut microbiome plays a vital role in the progression of many diseases, including cancer, due to increased inflammation in the body. Since gut microbiome-induced inflammation can serve as a novel therapeutic strategy, there is an increasing need to identify novel approaches to investigate the effect of inflammation instigated by gut microbiome on cancer cells. However, there are limited biomimetic co-culture systems that allow testing of the causal relationship of the microbiome on cancer cells. Here we developed a microfluidic chip that can simulate the interaction of the gut microbiome and cancer cells to investigate the effects …

Advanced Microstructural Characterization Of Functionally Graded Dental Ceramic Material For Materials-Informed Finishing, Angani Vigneswaran

Theses and Dissertations--Manufacturing Systems Engineering

Yttria-stabilized tetragonal zirconia polycrystal (Y-TZP) has gained popularity as the choice of material for dental prosthetics. Ivoclar Vivadent’s IPS e.max ZirCAD Prime dental ceramic incorporates a unique gradient technology that varies the yttria content over the thickness of the material. The top layer is composed of 5Y-TZP which is desired for its optical properties while the bottom layer is composed of a much stronger 3Y-TZP. In between the two layers, 5Y-TZP and 3Y-TZP are mixed to form a transition layer. Varying the amount of yttria allows for more esthetically pleasing translucency in the visible areas of the restoration without compromising …

Artificial Emotional Intelligence In Socially Assistive Robots, Hojjat Abdollahi

Electronic Theses and Dissertations

Artificial Emotional Intelligence (AEI) bridges the gap between humans and machines by demonstrating empathy and affection towards each other. This is achieved by evaluating the emotional state of human users, adapting the machine’s behavior to them, and hence giving an appropriate response to those emotions. AEI is part of a larger field of studies called Affective Computing. Affective computing is the integration of artificial intelligence, psychology, robotics, biometrics, and many more fields of study. The main component in AEI and affective computing is emotion, and how we can utilize emotion to create a more natural and productive relationship between humans …

A Case Study: Electronic Teaching Methods From The Pandemic Era That Students Still Appreciate, Douglas De Boer

ASEE North Midwest Section Annual Conference 2023 Publications

Background
The COVID-19 pandemic of 2020-2022 caused a rapid shift away from face-to-face instruction and toward online instruction. Due to the emergency speed with which the changes were rolled out results varied, desirable data on the quality of the changes was often not kept, and the retrospective study of what happened and the outcomes thereof is ongoing at the time this case study was written.
Purpose/Hypothesis
This case study is a comparison of the author’s experience with the current literature. It is hypothesized that some of the techniques used during the pandemic were effective and should be retained and/or further …