Digital Commons Network^™

Guiding 3d Chiral Self-Assembly At Nano/Micro Length Scales Via 3d Printing, Mohsen Esmaeili

Theses and Dissertations

This thesis delves into the intricate realm of 3D printing bio-inspired materials with tailored nano/microscale architectures. The first chapter investigates the flow-induced alignment of cellulose nanocrystals (CNC) during 3D printing, employing a microfluidic strategy integrated with polarized optical microscopy (POM). Birefringence measurements under varying conditions reveal the influence of initial CNC suspension structures on flow-induced patterns, particularly in the chiral nematic phase. Hydrodynamic simulations complement experimental findings, elucidating the impact on CNC particle alignment, providing insights for designing 3D printed architectures with internal chirality for advanced applications.

The second chapter explores the 3D printing of complex geometries inspired by Bouligand …

In-House Polydimethylsiloxane Microfluidic Device On Single Cell Trapping And Culturing Of Leukemia Cell Line: Comparison Between Conventional And Conventional With Centrifugal Methods, Alongkorn Pimpin, Sudchaya Bhanpattanakul, Tepparit Wongpakham, Chomkwan Tawatcharaporn, Thanawit Siha-Umphai, Mookdapa Sinsiriphan, Atikan Chalermchuang, Tewan Tongmanee, Attawut Thanomsridetchai, Thammawit Suwannaphan, Witsaroot Sripumkhaie, Pattaraluck Pattamang, Mayuree Chanasakulniyom, Praprudee Piyaviriyakul, Wutthinun Jeamsaksiri, Achariya Sailasuta, Theerawat Tharasanit, Theerayuth Kaewamatawong

The Thai Journal of Veterinary Medicine

To develop a suitable model to study biological and characterization of Cancer stem cells (CSCs), single cell trapping and culturing using microfluidic techniques have become a high-through put system that can manage and control microenvironment and practical intracellular analysis. The aim of this study was to develop and validate a microfluidic device for single cell trapping and culturing using the leukemia cell line as a cell model. Comparison between conventional and conventional with centrifugal methods was also analyzed. To compare trapping efficacy between these 2 methods, leukemia cell concentration of 1.5×106 cells/ml was loaded with a flow rate of 0.25 …

Cybersecurity, Image-Based Control, And Process Design And Instrumentation Selection, Dominic Messina, Akkarakaran Francis Leonard, Ryan Hightower, Kip Nieman, Renee O'Neill, Paloma Beacham, Katie Tyrrell, Muhammad Adnan, Helen E. Durand

Chemical Engineering and Materials Science Faculty Research Publications

Within an Industry 4.0 framework, a variety of new considerations are of increasing importance, such as securing processes against cyberattacks on the control systems or utilizing advances in image processing for image-based control. These new technologies impact relationships between process design and control. In this work, we discuss some of these potential relationships, beginning with a discussion of side channel attacks and what they suggest about ways of evaluating plant design and instrumentation selection, along with controller and security schemes, particularly as more data is collected and there is a move toward an industrial Internet of Things. Next, we highlight …

Zno Based Chemiresistive Sensor For Ammonia Detection, Anna Thomas

Theses and Dissertations

This work aims on the development of nanostructured sensing elements based on ZnO for detecting ammonia, specifically designed for applications in poultry and farm fields. To achieve this objective, we prepared sensing elements, including ZnO, CuO, WO3, NiO, ZnO/CuO, ZnO/WO3, ZnO/NiO, and rGO/ZnO, using the spray pyrolysis technique. Structural, morphological, and compositional analyses were studied using X-ray Diffraction (XRD), Field Emission Microscope (FESEM), and X-ray Photoelectron Spectroscopy (XPS), respectively. The vapour/gas detection studies of fabricated sensing elements were carried out in a home built sensing chamber.

The single oxides, ZnO, CuO, WO3, demonstrated ammonia detection in the range of 5-100 …

Mechanochemical Correlations And Spatial Distribution Of Components In Plant Cell Walls And Extracellular Matrices, Huiyong Li

McKelvey School of Engineering Graduate Student Theses & Dissertations

Plant cell walls and extracellular matrices (ECMs) are important to cellular activities in plant/animal organs. The organization of plant cell wall/ECM components (e.g., local composition, arrangement, and ultrastructure of cell wall/ECM polymers) plays a role in determining their local mechanical properties. As a result, the natural processes of cell and tissue growth, development, and function rely on the control of cell wall/ECM composition, arrangement, and ultrastructure to manipulate local mechanical properties. However, directly correlating cell wall/ECM composition, organization, and mechanics has been challenging. To overcome this barrier, one goal of this thesis is to develop probing methodologies at a sub-cellular …

Reinforcement Learning Based Planning And Control For Robotic Source Seeking Inspired By Fruit Flies, Gauravkumar Koradiya

Master's Theses

In the exploration of mobile sensing agents such as robots equipped with sensors, these agents have the potential to measure unknown scalar fields, ranging from chemical concentrations to temperature fluctuations. One of the primary interests is the robots’ ability to gravitate towards the peaks or troughs of these fields, a phenomenon termed as “source seeking”. This behavior is invaluable in practical scenarios, like detecting chemical leaks or locating survivors after catastrophes. However, the challenge becomes significantly more pronounced when operating in turbulent flow fields, where the erratic nature of odorant plumes makes predictions and simulations highly unreliable. Interestingly, fruit flies …

Purpose, Performance, And Process Influence On Airline Pilot Trust In Automation Technology: A Quantitative Study, Thomas Robert Meyer

Doctoral Dissertations and Projects

The purpose of this quantitative, descriptive survey study was to determine if purpose, performance, and process influence airline pilot trust in automation technology. The role of a tool is an extension of human capabilities. Initially limited to mechanical extensions of arms and legs, tools are more sophisticated and extend into mental abilities. Quantum leaps in computer and automation technology mitigate repetitive or complex calculations using developed cognitive processes. Through the Trust in Automation theoretical lens, this study used the Likert-based System Trustworthiness Scale offered online to approximately 3,000 airline pilots using simple random sampling methods comprising voluntary submissions. Data was …

Integrated Reference Single-Cell Rna Seq Atlas Of Healthy Human Female Tissues To Expand The Computational Drug Target Discovery Approaches For Female-Directed Non-Hormonal Contraceptives, Zhanel Nugmanova

Dartmouth College Master’s Theses

The ovary is vital for fertility and the maintenance of endocrine homeostasis. It contains heterogeneous cell populations, including oocyte, granulosa, stromal, endothelial, smooth muscle, and immune cell types. Creating a harmonized dataset that enables cross-tissue comparisons, including the ovary, is essential for identifying genes specific to ovarian cell types and for facilitating drug discovery for contraception and fertility purposes. There are very few resources that allow for the assessment of the expression of potential gene targets across human and mouse tissues for female-directed non-hormonal contraceptives. This is because most of these publicly available datasets are from post-menopausal women. Through our …

Modular Approach To Soft Mobile Robots, Dimuthu Kodippili Arachchige

College of Computing and Digital Media Dissertations

Soft robot locomotion is a highly promising but under-researched subfield within the field of soft robotics. The compliant limbs and bodies of soft robots offer numerous benefits, including the ability to regulate impacts, tolerate falls, and navigate through tight spaces. These robots have the potential to be used for various applications, such as search and rescue, inspection, surveillance, and more. The state-of-the-art still faces many challenges, including limited degrees of freedom, a lack of diversity in gait trajectories, insufficient limb dexterity, limited payload capabilities, lack of control methods, etc. To address these challenges, this research introduces a modular approach to …

Charlotte: A Modern Tool For Cave Surveying, Luca Tringali Mr., Giacomo Canciani Dr., Alexander Debenjak, Tecla Tripari

International Journal of Speleology

The future of cave surveying is a full 3D scan, as automatic as possible, capturing all morphologically relevant details. However, the vast majority of cavers are still using tools designed more than 15 years ago. The advancements in tools for architects, CAD designers, programmers of VR and robotics could be helpful also for the caving community. That’s why Charlotte, a cheap DIY 2.5D scanner designed for cave surveying, was developed and built. To demonstrate its capabilities, a new survey of Grotta Regina del Carso cave (cad. nr. 2328/4760VG), the biggest cave in the Gorizia Karst, has been realized and published. …

Predictive And Prescriptive Analytics For Minimizing The Cost Of Recidivism, Adreana Julander

Operations Research and Engineering Management Theses and Dissertations

A problem faced by the United States is the ever increasing prison population. There are inmates serving long sentences, new inmates being sentenced for the first time, and those who have previously served prison sentences that reoffend. The third group and the reduction of recidivism are the focus this dissertation. It is estimated that over 80% of inmates released from prison will reoffend within the next ten years. Is there an optimal sentence length that reduces that chance of an ex-convict reoffending? Are there programs or opportunities that some inmates have while incarcerated that reduce the probability they will return …

Design Of A Portable Fast Scan Cyclic Voltammetry Device Utilizing Pulse Width Modulation For Waveform Generation, Nora Szymkowski

Masters Theses

Fast Scan Cyclic Voltammetry (FSCV) is a widely used electrochemical technique for real-time measurement of the brain’s chemical messengers, including the molecule dopamine, with high temporal resolution. Currently the financial burden of performing FSCV is quite high, ranging from $8,000 to $20,000+ making the barrier to entry nearly insurmountable for laboratories and classrooms at small institutions. The purpose of this project was to develop a Do-It-Yourself (DIY), portable, and cost-effective FSCV system for use in laboratory and classroom settings. The project aimed to create a compact and cost-effective system that could be used by researchers and educators to study dopamine …

Thrombogenicity Of Biodegradable Metals, D. E.J. Anderson, H. H. Le, H. Vu, J. Johnson, J. E. Aslan, J. Goldman, M. T. Hinds

Michigan Tech Publications

Biodegradable metals offer a promising means to ameliorate many of the long-term risks associated with vascular devices made of conventional biostable stent metals. While numerous biodegradable metal alloys have been developed and characterized in animal models, knowledge of their blood reactivity and thrombogenicity remains unknown. Metal hemocompatibility is particularly valuable because current generation drug-eluting stents pose a significant long-term thrombosis risk. In this study, four pure metals, widely used as degradable base materials (Fe, Zn, Mg, and Mo), and three alloys commonly used in cardiovascular devices [NiTi, CoCr, and stainless steel (SS)] were evaluated. This work examined how each of …

Virtual Engagement: Can Law Enforcement Benefit From Utilizing Extended Reality In Their Training Systems?, Jacob Maxwell Read

Electronic Theses and Dissertations

Introduction: Extended reality (XR) technologies offer innovative training solutions for various industries, including healthcare, manufacturing, aviation, sports, military, and law enforcement. XR can provide a solution for training where job tasks are difficult or near impossible to train in real-life. A human factors research and design approach could provide an effective, low-risk, and cost and time-efficient option for integrating the use of XR technology into training for many industries. To advance this goal, this research answered two main research questions: (1) What training needs/challenges are best suited for an XR training system designed for law enforcement officers (LEOs)? (2) …

Epidural Stimulation For Urogenital Dysfunction In The Spinal Cord Injured Rat., Natasha Lorraine Wilkins

Electronic Theses and Dissertations

Spinal cord injury (SCI) is a devastating, life changing event that greatly impacts quality of life. SCI can have body-wide, multi-system consequences including dysfunction within the urogenital system. Challenges with the urinary system include urinary incontinence, urine retention, and detrusor-sphincter dyssynergia all of which require maintenance through the regular use of catheters. Sexual dysfunction in men, who comprise nearly 80% of the SCI population, includes erectile dysfunction, anejaculation, retrograde ejaculation, poor expulsive force during ejaculation, and poor sperm quality. The treatment for sexual function in SCI men is focused more on fertility through the collection of sperm for insemination procedures …

Natural Fiber Reinforced Composites For Sustainable Additive Manufacturing., Athira Nair Surendran

Electronic Theses and Dissertations

Carbon fiber is widely known for its exceptional strength, making it a sought-after material in composite manufacturing, especially with thermosets and thermoplastics. Its major applications span aerospace, wind turbines, sports equipment, automotive interior and exterior parts, robotics and medical equipment. Recently, carbon fiber filament manufacturing has gained attention due to the availability of desktop 3D printers and the versatility and customizability that comes with the realm of 3D printing. Commercial carbon fiber production, primarily from polyacrylonitrile (PAN), is energy-intensive and derived from crude oil, contributing to significant environmental impacts and poor recyclability. Alternatively, lignocellulosic natural fibers present a possible sustainable …

Expandable Tissue-Engineered Living Surgical Pulmonary Heart Valve For Pediatric Patients, Jacob M. Lautenschlager

All Theses

Cardiovascular disease is the most common cause of mortality in developed countries, with 607.74 million cases globally in 2020.^1,2 Advances in medicine have changed the current demographic suffering from heart valve disease into an aging population suffering from degenerative heart valve disease, creating a growing population treated by surgical or transcatheter intervention.^3-6 Current treatments of valvular heart disease are therefore directed toward valve replacements for the adult population, leaving a treatment gap for pediatric patients suffering from heart valve disease.^7-10

Congenital heart defects are defined as structural abnormalities of the heart or intrathoracic great vessels and are …

Feasibility Study For The Application Of A Solar Powered Absorption Chilling System For Cooling Poultry Houses In Northwest Arkansas, Matthew James Kelly

Graduate Theses and Dissertations

This paper investigates the economic feasibility of utilizing a solar-powered absorption chiller to provide cooling for poultry houses in northwest Arkansas. An existing thermodynamic model of a poultry house was modified to account for cooling. A simulation was performed using actual weather data from Fayetteville, Arkansas for 26 flocks of broilers at four cooling capacities for the 2020, 2021, and 2022 calendar years. Based on the simulation, the savings from reduced electricity use for ventilation and the cost of the required absorption chiller and solar thermal array for an 8-house farm were calculated. While providing cooling was found to reduce …

Applying Circuit Theory To Describe Changes In Structural Landscape Connectivity In Response To Wildfire, Christian Ross Nielsen

School of Natural Resources: Dissertations, Theses, and Student Research

Understanding and conserving ecological connectivity is critical to the preservation of vulnerable landscapes. Circuit theory, in which landscapes are imagined as circuit boards with varying resistances to the flow of current, is being increasingly used to model spatially explicit connectivity of landscapes and to inform land management and conservation decision-making. Utilizing continuous, quantitative estimates of percent cover by five land cover functional groups to create a conductance surface, this study expanded upon an established application of circuit theory that used the open-source software Circuitscape to model species-agnostic, omnidirectional connectivity. This model was automated using Python to create time-series connectivity maps …

Integration Of Matlab And Machine Learning To Accelerate Evaluation Of Biological Activity In Agricultural Soils And Promote Soil Health Improvement Goals, Andrew Stiven Ortiz Balsero

Department of Agricultural and Biological Systems Engineering: Dissertations, Theses, and Student Research

Traditionally, assessments of soil biological activity have been confined to laboratory settings, creating a disconnect with practical in-field methods. To bridge this gap, cotton fabric degradation has been used to illustrate soil microbial activity under different management practices. While effective, these demonstrations are subjective and labor-intensive.

Researchers have explored using image processing software like ImageJ and Adobe Photoshop to streamline this process. Although these tools accurately quantified fabric degradation under varying soil conditions, the methods remained labor-intensive and complex. Consequently, these methods were still not ideal for on-farm use by agricultural practitioners.

To further address labor and complexity limitations, the …

Image Processing Techniques For Water Droplet Penetration Time And Contact Angle Estimation, Sai Balaji Jai Kumar

UNLV Theses, Dissertations, Professional Papers, and Capstones

Water droplet behavior on soil surfaces plays a critical role in numerous environmental processes, including soil erosion, hydrological dynamics, and ecosystem health. Accurate characterization of soil water repellency, quantified by parameters such as water droplet penetration time (WDPT) and contact angles (WDCA), is essential for informed decision-making in agricultural management, forestry practices, and land-use planning. Despite the significance of these parameters, challenges exist in reliably estimating them due to the complex and dynamic nature of soil-water interactions. This thesis address challenges in estimating WDPT and WDCA, by leveraging state-of-the-art image processing techniques and machine learning algorithms. The research focuses on …

Modeling, Simulation, And Experimental Testing Of A Single-Legged Hopper, John Anthony Bennett

Master's Theses

Legged robots are well-suited for navigating unstructured terrain due to their ability to perform agile dynamic motions. Building on the efforts of Cal Poly's Legged Robotics Group, this project focuses on achieving stable forward hopping with a single legged robot.

A comprehensive model of leg hopping is formulated, with leg dynamics during flight and stance phases derived using Euler-Lagrange equations. During the stance phase, a spring-mass system models the leg-ground interaction. The touchdown and lift-off conditions are formulated mathematically, and state mappings between phases are determined via momentum conservation. A two-phase hybrid controller is designed, incorporating trajectory planning during flight …

Bioaffinity Analysis Of Aptamer-Target Interactions, Pooja Karunanithi

Honors Theses

This study explores the bioaffinity of aptamer-target interactions through a comprehensive analysis. Aptamers, oligonucleotides that can bind to target molecules, have emerged as powerful tools in various fields, including diagnostics and therapeutics. The primary objective of this research was to assess the binding affinity and specificity of selected aptamers to their respective targets using a combination of experimental techniques and computational models. The binding affinity of 10 different complexes were measured through surface plasmon resonance (SPR) followed by molecular simulations. The results showed that the 6th complex had the best binding affinity. The data suggest that these aptamers could be …

Integrating Building Information Modelling And Extended Reality In The Transportation Infrastructure Industry, Yosra El Maghraby, Shady Salem, Ahmed Alhady, Yasser Khodeir

Civil Engineering

No abstract provided.

On The Right Track? Energy Use, Carbon Emissions, And Intensities Of World Rail Transportation, 1840–2020, Bernardo Tostes, Sofia T. Henriques, Paul E. Brockway, Matthew Kuperus Heun, Tiago Domingos, Tânia Sousa

University Faculty Publications and Creative Works

The history of rail transport can offer valuable insights for future energy transitions due to its importance in promoting clean mobility. There is a complex interplay between the evolution of the railway network, fuel consumption, efficiency, energy service, and CO2 emissions that requires further exploration. We developed a dataset that covers energy use in all stages of rail transportation, as well as the length of track, energy service, and CO2 emissions at the world scale. To deal with missing data we utilized machine learning techniques for the first time in a historical energy reconstruction study. Our analysis reveals that …

Engineered Nucleic-Acid Binding Intrinsically Disordered Proteins For Biomedical Applications, Telmo Diez Perez

Biomedical Engineering ETDs

This dissertation explores the use of intrinsically disorder proteins (IDPs) in biomedical applications related to the detection and biosensing of nucleic-acid species. We engineer several low critical solution temperature (LCST) nucleic-acid binding elastin-like polypeptides (ELPs) as model IDPs and study their potential in biomedical application via interactions with short single-stranded (ss) DNA, tRNA and viral RNAs. First, we engineer a simple polycationic ELP capable of recruiting DNA on demand in a DNA-protein-rich condensate via LLPS. Moreover, we develop a modified Flory-Huggins theory to quantify the partitioning of DNA and protein upon coacervate formation with and without salts. We then design …

Carboxen Micropreconcentrator Performance For Analyzing Btex In Air., Raymond Daniel Spargo

Electronic Theses and Dissertations

Volatile Organic Compounds (VOCs) are a class of toxic chemicals in environmental air associated with several serious health risks. Some of the most common and concerning VOCs are Benzene, Toluene, Ethyl Benzene, and Xylene (BTEX). To maintain healthy air quality in these environments, researchers pursue instruments and processes to quickly and accurately predict sub-ppb concentrations of BTEX in indoor and outdoor air. By employing a micropreconcentrator (μPC) to combine Solid-Phase Micro-Extraction (SPME) with Gas Chromatography Mass Spectrometry (GC-MS) analysis, trace BTEX content in air samples can be captured, extracted and analyzed. In this thesis, a dual-compartment μPC with Carboxen 1000 …

The Impact Of Internal Curing Additives On Compressive Strength, Shrinkage, And Internal Relative Humidity Of Mortars., Payton Scott

Electronic Theses and Dissertations

Curing is a critical step in concrete construction. When a concrete specimen is properly cured, its shrinkage and early age curling/warping can be reduced, and the concrete strength can be increased. Curing methods can be divided into two categories: traditional external curing and emerging internal curing methods. Internal curing has similar benefits to external curing, such as reduced early-age cracking and shrinkage risk, and increased durability and strength. The purpose of this research is to investigate internal curing additives and their impact on length change, weight change, compressive strength, autogenous shrinkage, and internal relative humidity. Mortar mixtures with lightweight aggregate …

Development Of Recyclable Materials For Industry Using Non-Petroleum Feedstocks, Terra M. Miller-Cassman

Boise State University Theses and Dissertations

Plastics have been an essential material for over 80 years, yet we have been unable to manage the accumulation of plastic waste. The available solutions for recycling or replacing plastics are complex and costly, and thus have not kept pace with the increasing quantity of plastic waste. This dissertation describes a comprehensive, materials design approach to solving the current and future challenges of plastic waste. Recycled and recyclable materials are developed using commercial feedstocks to reduce barriers for industry adoption. In one approach, unsorted municipal solid waste is compressed at a low temperature and pressure to form rigid composite boards …

Tissue Engineered Models Of Environmental And Metabolism-Driven Cancer-Nerve Crosstalk, Emory Gregory

Graduate Theses and Dissertations

Purpose: Within the last 30 years, cancer-nerve interactions have garnered attention for causing patient pain, increasing the risk of recurrence, and decreasing patient prognosis. There are 2 key forms cancer-nerve crosstalk takes: perineural invasion (PNI), in which cancer cells infiltrate nerves as a route for metastasis, and tumor innervation (TI), in which neurites penetrate solid tumors to promote tumor progression. Present in vitro models of cancer-nerve crosstalk primarily include Matrigel® mouse matrix and collagen I hydrogels; whereas collagen I hydrogels neglect tumor extracellular matrix (ECM) complexity, Matrigel® is controversial for its batch-to-batch inconsistencies and inclusion of experiment-altering growth factors. Decellularized …