Digital Commons Network^™

Genes Differentially Expressed By Cumulus Cells And Assays Using Same To Identify Pregnancy Competent Oocytes, Jose B. Cibelli, Amy E. Iager, Hasan H. Otu

Department of Electrical and Computer Engineering: Faculty Publications

A genetic means of identifying "pregnancy competent" oocytes is provided. The means comprises detecting the level of expression of one or more genes that are expressed at characteristic levels (upregulated or downregulated) in cumulus cells derived from pregnancy competent oocytes. This characteristic gene expression level, or pattern referred to herein as the "pregnancy signature", also can be used to identify subjects with underlying conditions that impair or prevent the development of a viable pregnancy, e.g., pre-menopausal condition, other hormonal dysfunction, ovarian dysfunction, ovarian cyst, cancer or other cell proliferation disorder, autoimmune disease and the like. In preferred embodiments the …

Missouri River Recovery Program: Reclaiming Lost Habitat: Real Estate Program

United States Army Corps of Engineers, Omaha District

Historically, the Missouri River continually reshaped itself by eroding banks in some areas and building up islands and sandbars in other areas. The river’s dynamic nature provided plenty of habitat for native species of animals and plants.

Efforts in the 1900s to manage the river by building dams, levees and a navigation channel provided many social and economic benefits to the nation but drastically reduced the amount of habitat available for the river’s native species. Today, lack of habitat is a major reason that several Missouri River species are endangered or threatened, such as the pallid sturgeon, least tern and …

Switchgrass For Bioethanol And Other Value-Added Applications: A Review, Deepak R. Keshwani, Jay J. Cheng

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Switchgrass is a promising feedstock for value-added applications due to its high productivity, potentially low requirements for agricultural inputs and positive environmental impacts. The objective of this paper is to review published research on the conversion of switchgrass into bioethanol and other value-added products. Environmental benefits associated with switchgrass include the potential for carbon sequestration, nutrient recovery from runoff, soil remediation and provision of habitats for grassland birds. Pretreatment of switchgrass is required to improve the yields of fermentable sugars. Based on the type of pretreatment, glucose yields range from 70% to 90%, and xylose yields range from 70% to …

Sorghum Distillers Dried Grain Lipid Extract Increases Cholesterol Excretion And Decreases Plasma And Liver Cholesterol Concentration In Hamsters, Jia Tse Hoi, Curtis L. Weller, Vicki Schlegel, Susan L. Cuppett, Ji-Young Lee, Timothy P. Carr

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Grain sorghum is a rich source of phytochemicals. In this study, male hamsters were fed AIN-93M diets supplemented with a hexane-extractable lipid fraction from sorghum distillers dried grains with solubles (DDGS). Diets contained 0.0%, 0.5%, 1.0%, and 5.0% (w/w) DDGS lipid extract. After 4 wk, the 5.0% DDGS lipids group had significantly lower plasma non-HDL cholesterol and liver esterified cholesterol concentration. Fecal neutral sterol (i.e., cholesterol) excretion was significantly higher in the 5.0% DDGS lipids group compared to the other treatments (66% higher compared to controls). Bile acid excretion was not affected by DDGS lipid intake. Fecal cholesterol excretion was …

Antiproliferation Properties Of Grain Sorghum Dry Distiller’S Grain Lipids In Caco-2 Cells, Richard Zbasnik, Timothy P. Carr, Curtis L. Weller, Keum Taek Hwang, Lijun Wang, Susan L. Cuppett, Vicki Schlegel

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Antiproliferative properties of lipids extracted from grain sorghum (GS) dry distiller’s grain (DDG) were analyzed to determine the feasibility of developing GS coproducts as a source for human health dietary ingredients. The lipid extract of GSDDG was delivered to human colon carcinoma (Caco-2) cells by solubilizing 0−1000 μg/mL of GS-DDG lipids in 100 μg/mL increments with micelles. A significant reduction in cell viability (25−50%) resulted at treatment levels of 400−1000 μg/mL GS-DDG lipids (p < 0.05). Alternatively, total protein levels of cells treated with 400, 500, and 600 μg/mL of GS-DDG lipid were not significantly different from the control, indicating cell growth during the treatment period. Total cell counts for the control were not significantly different from the GS-DDG lipid treated cells, but dead cell counts increased by 10% for the latter sample with a concomitant increase of the intercellular protein lactate dehydrogenase leakage (30−40%) in the medium. Preliminary analysis by the fluorescence-activated cell method (FACs) demonstrated that nonviable cells were in either the early apoptotic, late apoptotic, or necrotic stage post-treatment with 400, 500, and 600 μg/mL GS-DDG lipids. Physiochemical characterization of the GS-DDG lipids used for the antiproliferation study showed the presence of vitamin E (predominantly γ-tocopherol), triacylglycerides (predominantly linoleic acid), policosanols, aldehydes, and sterols (predominantly campesterol and stigmasterol), each of which or as synergistic/additive group of constituents may be responsible for the antiproliferative effect.

Integration Of An Extended Octagonal Ring Transducer And Soil Coulterometer For Identifying Soil Compaction, Santosh Pitla, L. G. Wells, S. A. Shearer

Department of Agricultural and Biological Systems Engineering: Faculty Publications

The soil coulterometer is an “on‐the‐go” electro‐mechanical system which collects impedance force data at multiple depths using an oscillating coulter. During the initial testing (summer 2006), only vertical soil impedance force data was collected using a pressure sensor. To improve the performance of the coulterometer, an extended octagonal ring transducer was integrated into the system to collect both the horizontal and vertical impedance forces given by the soil. In the summer of 2007, data was collected using the revised sensor from a typical central Kentucky field setting in a 0.8‐ha (2‐acre) plot. Four passes were made with the coulterometer. Seventy …

N-Glycosylation Microheterogeneity And Site Occupancy Of An Asn-X-Cys Sequon In Plasma-Derived And Recombinant Protein C, Geun-Cheol Gil, William H. Velander, Kevin E. Van Cott

Kevin Van Cott Publications

Human protein C (hPC) is glycosylated at three Asn-X-Ser/Thr and one atypical Asn-X-Cys sequons. We have characterized the micro- and macro-heterogeneity of plasma-derived hPC and compared the glycosylation features with recombinant protein C (tg-PC) produced in a transgenic pig bioreactor from two animals having approximately tenfold different expression levels. The N-glycans of hPC are complex di- and tri-sialylated structures, and we measured 78% site occupancy at Asn-329 (the Asn-X-Cys sequon). The N-glycans of tg-PC are complex sialylated structures, but less branched and partially sialylated. The porcine mammary epithelial cells glycosylate the Asn-X-Cys sequon with a similar efficiency as human hepatocytes …

Rapid Development Of Multimedia Instructional Modules For Information Technology Education, Nanda Ganesan

Journal of International Technology and Information Management

A multifaceted learning model was developed for implementing e-learning in a largely commuter campus. The primary objective of the model was to build a flexible learning environment that combined the learning effectiveness of in-class learning with the flexibility and accessibility of online learning. One of the components of the model was the multimedia instructional modules produced to teach various Information Technology courses. Several different types of modules were produced representing chalk-and-talk type of lectures, PowerPoint presentations and software tutorials. The chalk-and-talk type of lecture modules and the software tutorials that emulated the in-class learning experience contributed positively towards enhancing the …

Bess: BIofuel ENergy SYstems SImulator: Life Cycle Energy & Emissions Analysis Model For Corn-Ethanol Biofuel Production Systems -- User’S Guide For The Bess Model, Adam Liska, Haishun Yang, Daniel T. Walters, Kenneth Cassman, Terry Klopfenstein, Galen Erickson, Virgil R. Bremer, Richard K. Koelsch, Dan Kenney, Patrick Tracy

Adam Liska Papers

The BESS model is a software tool to calculate the energy efficiency, greenhouse gas (GHG) emissions, and natural resource requirements of corn–to-ethanol biofuel production systems. The model provides a “cradle-to-grave” analysis of the production life cycle of biofuels from the creation of material inputs to finished products, producing an inventory of distributed GHG emissions from fossil fuels and a few key indirect emissions in the production life cycle. The model parameters can be set by the user to achieve the highest accuracy in evaluating a single corn-ethanol biorefinery and its surrounding feedstock crop production zone. The model equations and summary …

Analysis Of Possible Use Of Identification Technologies In Disassembly, Vukica Jovanović, Sreten Filipović, Gordana Ostojić, Stevan Stankovski, Milovan Lazarević

Engineering Technology Faculty Publications

The barcodes and Radio Frequency Identification tags that are being used during manufacturing, assembly, warehousing, and distribution could also be reused at the end of the product lifecycle for an identification of the correct disassembly process that would result in recycling of its components. The barcodes or RFID tags can be helpful in identifying objects in terms of sustainable development, regarding disassembly, recycling and reuse. In this paper, the analysis of the current applications of RFID Technology in the final phase of the product lifecycle will be presented. The simulation of the disassembly process supported by both barcode and RFID-enabled …

Aglite Lidar: A Portable Elastic Lidar System For Investivating Aerosol And Wind Motions At Or Around Agricultural Production Facilities, Christian C. Marchant, Thomas Wilkerson, Gail E. Bingham, Vladimir V. Zavyalov, Jan M. Andersen, Cordell B. Wright, Scott S. Cornelsen, Randy S. Martin, Philip J. Silva, Jerry L. Hatfield

Civil and Environmental Engineering Faculty Publications

The Aglite Lidar is a portable scanning lidar that can be quickly deployed at agricultural and other air quality study sites. The purpose of Aglite is to map the concentration of PM10 and PM2.5 in aerosol plumes from agricultural and other sources. Aglite uses a high-repetition rate low-pulse energy 3-wavelength YAG laser with photon-counting detection together with a steerable pointing mirror to measure aerosol concentration with high spatial and temporal resolution. Aglite has been used in field campaigns in Iowa, Utah and California. The instrument is described, and performance and lidar sensitivity data are presented. The value of the lidar …

Development Of Measurement And Modeling Techniques To Quantify Atmospheric Deposition Of Persistent, Bioaccumulative And Toxic Chemicals In The Great Lakes, Mark D. Rowe

Dissertations, Master's Theses and Master's Reports - Open

Measurement and modeling techniques were developed to improve over-water gaseous air-water exchange measurements for persistent bioaccumulative and toxic chemicals (PBTs). Analytical methods were applied to atmospheric measurements of hexachlorobenzene (HCB), polychlorinated biphenyls (PCBs), and polybrominated diphenyl ethers (PBDEs). Additionally, the sampling and analytical methods are well suited to study semivolatile organic compounds (SOCs) in air with applications related to secondary organic aerosol formation, urban, and indoor air quality.

A novel gas-phase cleanup method is described for use with thermal desorption methods for analysis of atmospheric SOCs using multicapillary denuders. The cleanup selectively removed hydrogen-bonding chemicals from samples, including much of …

Long-Range Pollution Transport: Trans-Atlantic Mechanisms And Lagrangian Modeling Methods, Robert Christopher Owen

Dissertations, Master's Theses and Master's Reports - Open

Over the past several decades, it has become apparent that anthropogenic activities have resulted in the large-scale enhancement of the levels of many trace gases throughout the troposphere. More recently, attention has been given to the transport pathway taken by these emissions as they are dispersed throughout the atmosphere. The transport pathway determines the physical characteristics of emissions plumes and therefore plays an important role in the chemical transformations that can occur downwind of source regions. For example, the production of ozone (O₃) is strongly dependent upon the transport its precursors undergo. O₃ can initially be formed …

An Algorithm For Identifying Novel Targets Of Transcription Factor Families: Application To Hypoxia-Inducible Factor 1 Targets, Yue Jiang, Bojan Cukic, Donald A. Adjeroh, Heath D. Skinner, Jie Lin, Qingxi J. Shen, Bing-Hua Jiang

Electrical & Computer Engineering Faculty Research

Efficient and effective analysis of the growing genomic databases requires the development of adequate computational tools. We introduce a fast method based on the suffix tree data structure for predicting novel targets of hypoxia-inducible factor 1 (HIF-1) from huge genome databases. The suffix tree data structure has two powerful applications here: one is to extract unknown patterns from multiple strings/sequences in linear time; the other is to search multiple strings/sequences using multiple patterns in linear time. Using 15 known HIF-1 target gene sequences as a training set, we extracted 105 common patterns that all occur in the 15 training genes …

Formation Feedback Control Of Uav Flight, Stephen Stegall

Doctoral Dissertations and Master's Theses

This thesis is a study of formation control with autonomous unmanned aerial vehicles using the formation as feedback. There is also an investigation of formation methods presenting insight into different algorithms for formations. A rigid formation is achieved using a proportional-derivative virtual structure with a formation feedback controller. There is an emphasis on stick controlled aerodynamics. The rigid formation is verified by a simulation of a longitudinal model. Formation control ideas are presented for rigid formations.

An Experimental And Numerical Investigation Of Flapping And Plunging Wings, Taylor A. Swanson

Doctoral Dissertations

"Micro air vehicles, or MAVs, are of current interest for a multitude of uses to which they, being small, unmanned vehicles, are uniquely suited. Among the proposed uses are exploration, reconnaissance, and communications. They can be deployed inside buildings, where their small size, hovering capability, and maneuverability, are important factors. Due to their small size, they operate at low Reynolds numbers where conventional flying mechanisms are not advantageous. Thus, attempts have been made to learn from natural flyers like insects and birds. Natural flight is accomplished by flapping wings, and this idea has been proposed for certain types of MAVs …

Using The Taguchi Design And The Central Composite Design To Increase The Robustness Of A Process From Its Raw Material Variability, Alvaro Cuevas

Open Access Theses & Dissertations

This research presents an experimental study where four controllable and one noise factor are analyzed using the Taguchi approach and the Central Composite Design (CCD) in order to induce robustness in a process that is being designed to treat residual grease from small restaurants. Three response variables are analyzed separately to establish the parameters conditions to obtain the most robust process output. These suggested conditions are validated conducting confirmation runs that are compared statistically to the predictions from the Taguchi prediction model for the Taguchi approach, and the Response optimizer for the CCD. The accuracy of each method is measured …

Spectroscopic Characterizations Of Organic/Inorganic Nanocomposites, Jayesh Rasikalal Govani

Open Access Theses & Dissertations

Inorganic potassium dihydrogen phosphate (KDP) is widely known for its value as a nonlinear optical material. In this study, pure and l-arginine-doped KDP single crystals were grown by the slow solvent evaporation technique and further subjected to infrared absorption and Raman studies for the confirmation of chemical group functionalization and possible bonding between the organic and inorganic materials. The appearance in the infrared absorption spectra of additional vibrational lines, which mostly originate from disturbed N-H, C-H, and C-N bonds of the l-arginine-doped salt, confirm the interaction between KDP and the organic material. This affirmation is supported by more evidence from …

Characterization And Cytotoxic Assessment Of Ballistic Aerosolized Particulates For Tungsten Alloy Penetrators Interacting With Steel Targets, Brenda Ivette Machado

Open Access Theses & Dissertations

The aerosolized particulates produced during an impact of depleted uranium munitions can potentially contaminate extensive areas around the impact sites and cause a wide spectrum of civilian and military damage. Thus materials such as WHA have been prompted for consideration. The present research is concerned with the regular collection of aerosol particulates associated with ballistic WHA rod penetration into rolled homogeneous steel (RHA) or other related steel armor as armor plate sequences. Particulate size characterization via scanning electron microscopy (SEM) and transmission electron microscopy (TEM) along with chemical compositions via energy dispersive spectroscopy (EDS) were the focus of this research. …

Retention And Productivity In Industrial Search And Scan Tasks, Camilo Aguilera

Open Access Theses & Dissertations

An experiment was developed in order to study the interaction between individual factors (gender and mood) and the retention ability in search and scan activities. 54 subjects between 18 and 30 years old participated in a computer-based memory test from the North of Mexico area. A full factorial design was used to analyze the variables. Two factors, one with two levels (gender: male and female), and the second factor with three levels (mood: positive, neutral, and negative) were used in the analysis. The output was measured as the amount of characters memorized in a string of random alphanumeric characters. Data …

Multi-Objective Network Reliability Optimization Using Evolutionary Algorithms, Franciso Oswaldo Aguirre

Open Access Theses & Dissertations

This work presents a new multiple objective evolutionary algorithm to solve three well known network reliability allocation problems considering different conflicting objectives to be optimized simultaneously. The new algorithm is applied in the design of a telecommunication network that is formed for several stations or nodes interconnected by telecommunication links or paths. The problem presented in this work involves finding which links to activate in order to obtain connectivity in the nodes. The number of nodes that need to be connected depends of the case that is being evaluated. The three network reliability problems considered are: all-terminal, k-terminal, and two-terminal. …

Art In A New Light: Design And Assessment Of Illuminants To Reduce Photochemical Degradation Of Works Of Art, Monica Fabiola Delgado

Open Access Theses & Dissertations

The purpose of this research is to design the best lighting that will minimize long term photochemical degradation of Old Master Drawings and/or other works of art, while maintaining the patron's appreciation of the object's color and detail. The present approach is a technological refinement to the basic underlying earlier work on fluorescent lighting by W. A. Thornton, W.A. at General Electric1. Thin-film dielectric, multi-coating technology is used to create filters that eliminate ultraviolet light, near infrared light and significant unnecessary parts from the visible spectrum, thus maximizing the reduction in photochemical degradation, while maintaining optimal color rendering. Three interference …

Retrofitting A Stereolithography System Within A Laminar Flow Hood, Mahesh Pandurang Tonde

Open Access Theses & Dissertations

This research focused on retrofitting the stereolithography (SL), a rapid prototyping technology, inside a laminar flow hood to provide an aseptic, particulate-free environment for scaffold fabrication. Two of the important objectives of this project were to retrofit the commercial SLA 250/30 inside a laminar flow hood to demonstrate that the manufacturability of the Bio-SL system was comparable to original SLA 250 system and to conduct the sterility test on fabricated scaffolds to show that the laminar flow hood was able to provide an aseptic, particulate-free environment for the fabrication.

Autonomous Vehicle Navigation: A Comparative Study Of Classical Logic And Neural Network Technique, Javier Alejandro Flores

Open Access Theses & Dissertations

Today is an era in which humans need the help of automated machines to facilitate their hectic lives. Over the past few decades, robotics has been one of the most researched areas in the world. One area of this research is obstacle avoidance for autonomous vehicles. In order to avoid an obstacle, the system may include two important characteristics: obstacle detection and avoidance control. In this Thesis a classical logic system and artificial neural network approach is presented. With the help of an Integrated Development Environment, a virtual robot was able to negotiate a maze and avoid obstacles according to …

Exploring Poly(Ethylene Glycol) As A Suitable Material For Peripheral Nerve Regeneration Scaffolds Manufactured By Stereolithography, Nubia Zuverza

Open Access Theses & Dissertations

One of the challenges in tissue engineering is to have spatial and temporal control over the biological elements within a scaffold used to guide regeneration for example of transected nerves. Some of the physical and chemical characteristics to regulate include incorporation of bioactive domains and release of chemical signals. This study presents the use of stereolithography (SL) to incorporate localized domains for cell adhesion in addition to include releasable nerve growth factor (NGF) in the process of building poly(ethylene glycol)diacrylate (PEGda) hydrogel scaffolds. Besides providing sites for cell attachment, an ideal nerve guidance conduit (NGC) should be able to release …

Regeneration Of Damaged Growth Plate Using Igf-I Plasmid-Releasing Porous Plga Scaffolds, Nirmal Ravi

University of Kentucky Doctoral Dissertations

Growth plate injuries account for 15-30% of long bone fractures in children. About 10% of these result in significant growth disturbances due to formation of a boney bar. If not treated correctly, this can lead to life-lasting consequences of limb length inequalities and angular deformities. Current treatments for growth plate injuries include removal of boney bar and insertion of fat, silicone, bone cement, etc.. This treatment y is inadequate, leaving almost half of these patients with continued deformities. This dissertation reports characterization of a DNA–containing porous poly(lactic-co-glycolic acid) (PLGA) scaffold system, chondrogenesis using insulin-like growth factor I (IGF-I) plasmid-releasing scaffolds …

Online Child Sexual Abuse: The French Response, Mohamed Chawki

Journal of Digital Forensics, Security and Law

Online child sexual abuse is an increasingly visible problem in society today. The introduction, growth and utilization of information and telecommunication technologies (ICTs) have been accompanied by an increase in illegal activities. With respect to cyberspace the Internet is an attractive environment to sex offenders. In addition to giving them greater access to minors, extending their reach from a limited geographical area to victims all around the world, it allows criminals to alter or conceal their identities. Sexual predators, stalkers, child pornographers and child traffickers can use various concealment techniques to make it more difficult for investigators to identify them …

Agent Based Modeling In Computer Graphics And Games, Brian Macnamee

Books/Book Chapters

As graphics technology has improved in recent years, more and more importance has been placed on the behavior of virtual characters in applications set in virtual worlds in areas such as games, movies and simulations. The behavior of virtual characters should be believable in order to create the illusion that these virtual worlds are populated with living characters. This has led to the application of agent-based modeling to the control of these virtual characters. There are a number of advantages of using agent-based modeling techniques which include the fact that they remove the requirement for hand controlling all agents in …

Renewable Energy Resources And Technologies Applicable To Ireland, Fergal O'Rourke, Fergal Boyle, Anthony Reynolds

Articles

The energy consumed in Ireland is primarily achieved by the combustion of fossil fuels. Ireland’s only indigenous fossil fuel is peat; all other fossil fuels are imported. As well as fossil fuels continually becoming more expensive, their use as an energy source also has a negative impact on the environment. Ireland’s energy consumption can be separated into 3 divisions; transportation, electricity generation and heat energy. Ireland however has a vast range of high quality renewable energy resources. Ireland has set a target of 33% of its electricity will be generated from renewable sources by 2020[1]. The use of biomass, wind …

Freeze Casting Of Bioactive Glass And Ceramic Scaffolds For Bone Tissue Engineering, Qiang Fu

Doctoral Dissertations

"The main objectives of this dissertation were to explore the production of bioactive ceramic and glass scaffolds with oriented pore architectures by unidirectional freezing of suspensions, and to characterize the mechanical and biological performance of the scaffolds. Freezing of aqueous suspensions of hydroxyapatite (HA) or bioactive 13-93 glass particles resulted in the formation of scaffolds with a lamellar-type microstructure (pore width = 5-30 µm). The addition of polar organic solvents (such as 60 wt% dioxane) to the aqueous suspensions markedly changed the morphology and size of the oriented pores, giving scaffolds with a columnar-type microstructure and larger pre width (90-110 …