Digital Commons Network^™

Plasma Mediated Molecular Delivery, Richard J. Connolly

USF Tampa Graduate Theses and Dissertations

Non-viral delivery of plasmid DNA has traditionally relied upon physical forces applied directly to target tissues. These physical methods typically involve contact between an applicator and the target tissue and often cause transient patient discomfort. To overcome the contact-dependent limitations of such delivery methodologies, an atmospheric direct current plasma source was developed to deposit ionized gas molecules onto localized treatment sites. The deposition of charged species onto a treatment site can lead to the establishment of an electric field with strengths similar to those used for traditional electroporation. In vitro experiments proved that this technology could transiently permeabilize cell membranes …

A Single-Chip Ultra-Wideband Based Wireless Sensor Network Node, Nathan R. Schemm

Department of Electrical and Computer Engineering: Dissertations, Theses, and Student Research

This dissertation presents the design of a next-generation wireless sensor network node. The node incorporates many new and innovative technologies such as an ultra-wideband radio which allows very low-energy communication, a low-power radiation detection front end, and an efficient implementation of dynamic voltage scaling which improves the energy efficiency of the integrated processor. The complete design is integrated on a single chip for maximum power savings and minimal size.

The ultra-wideband transceiver includes many novel techniques to produce a receiver with low power consumption and fast and accurate packet acquisition and reception. These include the use of a standard symmetric …

Design And Fabrication Of A Membrane Integrated Microfluidic Cell Culture Device Suitable For High-Resolution Imaging, Alla A. Epshteyn

USF Tampa Graduate Theses and Dissertations

Malaria remains a serious concern for people living and traveling to warm climates in Africa, Asia, and some parts of America. Understanding the mechanism of the malaria parasite in the liver phase could lead to important discoveries for preventative and treatment therapeutics before the disease develops into the blood stage. While in vitro liver cell culture models have been explored, a device that mimics the liver cell architecture with the capability of high-resolution imaging has never been created. In this research, a cell culture microfluidic device was designed and fabricated with a membrane integrated design to mimic the architecture of …

Virtual Blasting System For Removal Of Coating And/Or Rust From A Virtual Surface, Michael Bolick, Chris Lampe, Jason Ebensberger, Jeremiah Treloar, Rick Klein, Eric Conrad Peterson, Chad Jason Zalkin

Patents (University of Northern Iowa)

A computer simulation and virtual reality system simulates the use of a blasting nozzle to remove one or more coatings and/or rust from a virtual surface. The user operates an electronic controller in the form of a blasting nozzle that outputs a signal indicating whether the blasting nozzle controller is in an "on" position or in an "off" position. The system also has a motion tracking system that tracks the position and orientation of the blasting nozzle controller with respect to the virtual surface defined on the display screen. Simulation software in a computer generates virtual blast pattern data, and …

Perpetual Exploration Of Relational Information And Enhanced Star Glyphs For Multi-Source Data Visualization, Andrew Crowell

Theses and Dissertations

Information Visualization is an area of research concerned with the presentation of abstract data in a visual format. It has many diverse applications in fields of software engineering, information sciences, biology, chemistry, and medical, among others. This thesis is primarily concerned with the application of information visualization to the display of directed graphs and to the display of multivariate data for analysis. Two novel applications will be presented that are both advancements in the field of information visualization. The first application applies to the visualization and navigation of large or infinite graphs and networks. The quality of a graph drawing …

The Virtual Hip: An Anatomically Accurate Finite Element Model Based On The Visible Human Dataset, Jonathan M. Ford

USF Tampa Graduate Theses and Dissertations

The purpose of this study is to determine if element decimation of a 3-D anatomical model affects the results of Finite Element Analysis (FEA). FEA has been increasingly applied to the biological and medical sciences. In order for an anatomical model to successfully run in FEA, the 3-D model’s complex geometry must be simplified, resulting in a loss of anatomical detail. The process of decimation reduces the number of elements within the structure and creates a simpler approximation of the model. Using the National Library of Medicine’s Visible Human Male dataset, a virtual 3-D representation of several structures of the …

Assessment Of State Recycling Regulations In The United States, Nazli Yesiller, James L. Hanson, Samuel A. Vigil

Civil and Environmental Engineering

This investigation was conducted to identify the current status of state recycling regulations in U.S. using an extensive survey. Questions were included regarding: baseline waste generation and recycling trends; recycling regulatory trends; quantitative thresholds; integration of science, engineering, and economic principles; material properties; and legislative status of regulations. The majority of the surveyed states had regulations for recycling activities, which were less strict than solid waste regulations, and required permits for operation of recycling facilities. A low percentage of the states regulated generators, transporters, and handlers and a high percentage regulated recyclers. In general, transfer stations and recycling centers were …

Surface Morphology Of Platelet Adhesion Influenced By Activators, Inhibitors And Shear Stress, Melanie Groan Watson

Doctoral Dissertations

Platelet activation involves multiple events, one of which is the generation and release of nitric oxide (NO), a platelet aggregation inhibitor. Platelets simultaneously send and receive various agents that promote a positive and negative feedback control system during hemostasis. Although the purpose of platelet-derived NO is not fully understood, NO is known to inhibit platelet recruitment. NO's relatively large diffusion coefficient allows it to diffuse more rapidly than platelet agonists. It may thus be able to inhibit recruitment of platelets near the periphery of a growing thrombus before agonists have substantially accumulated in those regions.

Results from two studies in …

A Realistic Technology And Engineering Assessment Of Algae Biofuel Production, T. J. Lundquist, I. C. Woertz, N. W. T. Quinn, J. R. Benemann

Civil and Environmental Engineering

This report assesses the economics of microalgae biofuels production through an analysis of five production scenarios. These scenarios, or cases, are based on technologies that currently exist or are expected to become available in the near-term, including raceway ponds for microalgae cultivation, bioflocculation for algae harvesting, and hexane for extraction of algae oil. Process flow diagrams, facility site layouts, and estimates for the capital and operations costs of each case were developed de novo. This report also reviews current and developing microalgae biofuel technologies for both oil and biogas production, provides an initial assessment of the US and California …

An Improved Layer-By-Layer Self-Assembly Technique To Generate Biointerfaces For Platelet Adhesion Studies: Dynamic Lbl, Juan Manuel Lopez

Doctoral Dissertations

Layer-by-layer self-assembly (LbL) is a technique that generates engineered nano-scale films, coatings, and particles. These nanoscale films have recently been used in multiple biomedical applications. Concurrently, microfabrication methods and advances in microfluidics are being developed and combined to create "Lab-on-a-Chip" technologies. The potential to perform complex biological assays in vitro as a first-line screening technique before moving on to animal models has made the concept of lab on a chip a valuable research tool.

Prior studies in the Biofluids Laboratory at Louisiana Tech have used layer-by-layer and in vitro biological assays to study thrombogenesis in a controlled, repeatable, engineered environment. …

Analysis Of The Detection Of Organophosphate Pesticides In Aqueous Solutions Using Polymer-Coated Sh-Saw Devices, Arnold Kweku Mensah-Brown

Dissertations (1934 -)

Organophosphate pesticides (OPs) have been found as contaminants in surface and ground waters, soil, and agricultural products. Because OPs are toxic compounds, rapid detection/monitoring of OPs in groundwater is necessary to allow for real-time remediation. Detection of OPs in water has already been demonstrated using poly(epichlorohydrin) [PECH] and polyurethane as the sensing layers. However, the response times were relatively long, hindering real-time monitoring. In this work, a hybrid organic/inorganic chemically sensitive layer [bisphenol A-hexamethyltrisiloxane (BPA-HMTS)] that shows a high degree of partial selectivity for OPs is synthesized, characterized (in terms of the glass transition temperature, T_g, water stability, …

Alterations In Multiple Measures Of White Matter Integrity In Normal Women At High Risk For Alzheimer's Disease, Brian T. Gold, David K. Powell, Anders H. Andersen, Charles D. Smith

Neuroscience Faculty Publications

There is evidence that disruption of white matter (WM) microstructure is an early event in the course of Alzheimer's disease (AD). However, the neurobiological bases of WM microstructural declines in presymptomatic AD are unknown. In the present study we address this issue using a multimodal imaging approach to the study of presymptomatic AD. Participants were 37 high-risk (both family history of dementia and one or more APOE4 alleles) women and 20 low-risk (neither family history nor APOE4) women. Groups were matched for age, education, neuropsychological performance, and vascular factors that could affect white matter. Whole-brain analyses of diffusion tensor imaging …

The Geometric Growth Of M&S Education: Pushing Forward, Pushing Outward, John A. Sokolowski, Catherine M. Banks

VMASC Publications

M&S education is experiencing a geometric growth that is placing it front and center as a discipline and as an interdisciplinary tool. This article discusses the evolution of the discipline of M&S. It answers: Why has M&S education experienced a forward and outward growth? What is it that makes this discipline unique? What is the current state of M&S education?

Detrimental Thoracoabdominal Interaction With Lateral Airbag Restraints, Jason John Hallman

Dissertations (1934 -)

Side impact motor vehicle crashes pose unique challenges for occupant protection, particularly with regard to torso injury mitigation. The minimal crush distance between the vehicle exterior and the occupant torso has necessitated advanced passive safety technologies in response to tightened regulatory requirements and increased public awareness of safety issues. In particular, lateral airbag restraints (side airbags) have undergone a rapid and unregulated introduction in recent years, with US availability increasing to over 90% of new vehicles in 2010. As with frontal airbag restraints, the prdissertationsity for injury to occupants in close proximity to side airbag deployment remains a concern. Test …

Development Of A Unique Whole-Brain Model For Upper Extremity Neuroprosthetic Control, Dominic Nathan

Dissertations (1934 -)

Neuroprostheses are at the forefront of upper extremity function restoration. However, contemporary controllers of these neuroprostheses do not adequately address the natural brain strategies related to planning, execution and mediation of upper extremity movements. These lead to restrictions in providing complete and lasting restoration of function. This dissertation develops a novel whole-brain model of neuronal activation with the goal of providing a robust platform for an improved upper extremity neuroprosthetic controller. Experiments (N=36 total) used goal-oriented upper extremity movements with real-world objects in an MRI scanner while measuring brain activation during functional magnetic resonance imaging (fMRI). The resulting data was …

Co-Creation And Collaboration In A Virtual World: A 3d Visualization Design Project In Second Life, Keng Siau, Fiona Fui-Hoon Nah, B. Mennecke, S. Schiller

Research Collection School Of Computing and Information Systems

One of the most successful and useful implementations of 3D virtual worlds is in the area of education and training. This paper discusses the use of virtual worlds in education and describes an innovative 3D visualization design project using one of the most popular virtual worlds, Second Life. This ongoing project is a partnership between IBM and three universities in the United States: the University of Nebraska-Lincoln, Iowa State University, and Wright State University. More than 400 MBA students have participated in this project by completing a creative design project that involves co-creation and collaboration in Second Life. The MBA …

Development Of A Tool For Windfarm Site Analysis In Electrical Networks With High Levels Of Wind Power Generation, Edward Carroll, John Mcgrory

Dissertations

Controlling electrical networks that contain high levels of wind power will be a challenge facing many System Operators in the coming years. The intermittency and uncertainty of the power supplied by wind turbines create difficulties surrounding scheduling of plant, operating reserve and grid integration. The current process for windfarm selection is carried out by an individual stakeholder and lacks the consideration for all other stakeholders involved, which can create problems in the long term when the levels of wind power increase on the network. This paper proposes a collective approach to analysing potential windfarm sites using the key factors which …

Dispersion Of Cytotoxic Properties Of Multi-Walled Carbon Nanotubes Suspended In Biological Solutions With Tween 80: Their Role In Enhancing Killing Effects Of Nanosecond Pulse Electric Fields On Tumor Cell Lines, Bhargava S. Kalluri

Biological Sciences Theses & Dissertations

The objective of this study was to determine whether multi-walled carbon nanotubes (MWCNTs) suspended in the surfactant Tween 80 give an additive killing effect on tumor cells when exposed to nsPEFs. In this study, MWCNTs were suspended in DMEM and RPMI with or without T80 (surfactant). The size distribution of MWCNTs suspended in these solutions was evaluated with a Delsa™ Nano Zeta potential and sub micro particle Size Analyzer and confirmed with microscopy. The cytotoxicity of MWCNTs dispersed in different concentrations of T80 was evaluated in PANC1 (Human pancreatic cancer cell line) and Jurkat cell lines (Human T-cell lymphoblast cell …

Prediction Of Shock Diamonds And Mach Disk In An Under-Expanded Air Jet, Praveen D. Malali

Mechanical & Aerospace Engineering Theses & Dissertations

The present study involves performing simulations, using the commercially available CFD code Fluent, to obtain under-expanded jet flow structures for different values of nozzle pressure ratio (NPR). A fluid flow domain comprised of a converging nozzle attached to a plenum chamber is discretized, using triangular cells, in the pre-processing software Gambit, companion software to Fluent. The test of grid independence is carried out by performing simulations for NPR value of 10 using three discretized flow domains, referred to as a mesh or a grid, with varying cell numbers. Two models, namely the inviscid compressible flow model, employing Euler equations, and …

Biological Computation, Melanie Mitchell

Computer Science Faculty Publications and Presentations

In this article, the term biological computation refers to the proposal that living organisms themselves perform computations, and, more specifically, that the abstract ideas of information and computation may be key to understanding biology in a more unified manner. It is important to point out that the study of biological computation is typically not the focus of the field of computational biology, which applies computing tools to the solution of specific biological problems. Likewise, biological computation is distinct from the field of biologically-inspired computing, which borrows ideas from biological systems such as the brain, insect colonies, and the immune system …

Fetal Breathing Movement And Its Distinctive Characteristics In Bio-Magnetic Data, Umit Deniz Ulusar

Theses and Dissertations

Magnetoencephalography (MEG) is widely used to study magnetic fields generated by the electrical activity of the human brain and can also be applied for fetal neurological health assessment. The fetal MEG (fMEG) signals are often contaminated with other bio-magnetic signals such as fetal breathing movement (FBM), which acts as an interfering factor for fetal neurological signals. This study was carried out to identify the bio-magnetic signals pertinent to the FBM. The fMEG signals were captured using superconducting quantum interference devices (SQUIDs) and the FBMs were recorded by an ultrasound-based video technique. The simultaneous recording is challenging since SQUIDs are extremely …

Providing A Theoretical Basis For Nanotoxicity Risk Analysis Departing From Traditional Physiologically-Based Pharmacokinetic (Pbpk) Modeling, Dirk P. Yamamoto

Theses and Dissertations

The same novel properties of engineered nanoparticles that make them attractive may also present unique exposure risks. But, the traditional physiologically-based pharmacokinetic (PBPK) modeling assumption of instantaneous equilibration likely does not apply to nanoparticles. This simulation-based research begins with development of a model that includes diffusion, active transport, and carrier mediated transport. An eigenvalue analysis methodology was developed to examine model behavior to focus future research. Simulations using the physicochemical properties of size, shape, surface coating, and surface charge were performed and an equation was determined which estimates area under the curve for arterial blood concentration, which is a surrogate …

Analysis Of Alternative Methods For Long-Term Wind Speed And Initial Site Assessment For Purposes Of Wind Energy Production Estimation, Ryan Mcdevitt

Theses and Dissertations

A need exists for identification and evaluation of viable sites for wind energy. However, this is made difficult by the site-specific nature of the wind. A review of current industry practices shows that linear regression is the preferred method, although little work has been done to investigate alternative approaches. To address this, various methods were tested on 23 target sites with data measured over a long-term period. Each site was analyzed with the chosen best reference station, across 10 data periods for each method with results classified as mean absolute percentage error in energy density between the projected and measured …

From Interval And Probabilistic Granules To Granules Of Higher Order, Vladik Kreinovich

Departmental Technical Reports (CS)

Constructive mathematics, mathematics in which the existence of an object means that that we can actually construct this object, started as a heavily restricted version of mathematics, a version in which many commonly used mathematical techniques (like the Law of Excluded Middle) were forbidden to maintain constructivity. Eventually, it turned out that not only constructive mathematics is not a weakened version of the classical one -- as it was originally perceived -- but that, vice versa, classical mathematics can be viewed as a particular (thus, weaker) case of the constructive one. Crucial results in this direction were obtained by M. …

Physics Lesson Plan: How Far And Fast Does It Travel?, Getaway Special Team 2010

Education and Outreach

No abstract provided.

A Simple Apparatus For Measuring Cell Settling Velocity, Zhaowei Wang, Joanne M. Belovich

Chemical & Biomedical Engineering Faculty Publications

Accurate cell settling velocity determination is critical for perfusion culture using a gravity settler for cell retention. We have developed a simple apparatus (a 'settling column') for measuring settling velocity and have validated the procedure with 15-μm polystyrene particles with known physical properties. The measured settling velocity of the polystyrene particles is within 4% of the value obtained using the traditional Stokes' law approach. The settling velocities of three hybridoma cell lines were measured, resulting in up to twofold variation among cell lines, and the values decreased as the cell culture aged. The settling velocities of the nonviable cells were …

Quantitative Imaging Reveals Heterogeneous Growth Dynamics And Treatment-Dependent Residual Tumor Distributions In A Three-Dimensional Ovarian Cancer Model, Jonathan P. Celli, Imran Rizvi, Conor L. Evans, Adnan O. Abu-Yousif, Tayyaba Hasan

Dartmouth Scholarship

Three-dimensional tumor models have emerged as valuable in vitro research tools, though the power of such systems as quantitative reporters of tumor growth and treatment response has not been adequately explored. We introduce an approach combining a 3-D model of disseminated ovarian cancer with high-throughput processing of image data for quantification of growth characteristics and cytotoxic response. We developed custom MATLAB routines to analyze longitudinally acquired dark-field microscopy images containing thousands of 3-D nodules. These data reveal a reproducible bimodal log-normal size distribution. Growth behavior is driven by migration and assembly, causing an exponential decay in spatial density concomitant with …

Differential Regulation Of Immature Articular Cartilage Compressive Moduli And Poisson’S Ratios By In Vitro Stimulation With Igf-1 And Tgf-Β1, Gregory M. Williams, Kristin J. Dills, Christian R. Flores, Michael E. Stender, Kevin M. Stewart, Lauren M. Nelson, Albert C. Chen, Koichi Masuda, Scott J. Hazelwood, Stephen M. Klisch, Robert L. Sah

Mechanical Engineering

Mechanisms of articular cartilage growth and maturation have been elucidated by studying composition-function dynamics during in vivo development and in vitro culture with stimuli such as insulin-like growth factor-1 (IGF-1) and transforming growth factor-beta 1 (TGF-β1). This study tested the hypothesis that IGF-1 and TGF-β1 regulate immature cartilage compressive moduli and Poisson’s ratios in a manner consistent with known effects on tensile properties. Bovine calf articular cartilage from superficial-articular (S) and middle-growth (M) regions were analyzed fresh or following culture in medium with IGF-1 or TGF-β1. Mechanical properties in confined (CC) and unconfined (UCC) compression, cartilage matrix composition, and explant …

Differential Regulation Of Immature Articular Cartilage Compressive Moduli And Poisson's Ratios By In Vitro Stimulation With Igf-1 And Tgf-Β1, Gregory M. Williams, Kristin J. Dills, Christian R. Flores, Michael E. Stender, Kevin M. Stewart, Lauren M. Nelson, Albert C. Chen, Koichi Masuda, Scott J. Hazelwood, Stephen M. Klisch, Robert L. Sah

Biomedical Engineering

Mechanisms of articular cartilage growth and maturation have been elucidated by studying composition-function dynamics during in vivo development and in vitro culture with stimuli such as insulin-like growth factor-1 (IGF-1) and transforming growth factor-beta 1 (TGF-β1). This study tested the hypothesis that IGF-1 and TGF-β1 regulate immature cartilage compressive moduli and Poisson’s ratios in a manner consistent with known effects on tensile properties. Bovine calf articular cartilage from superficial-articular (S) and middle-growth (M) regions were analyzed fresh or following culture in medium with IGF-1 or TGF-β1. Mechanical properties in confined (CC) and unconfined (UCC) compression, cartilage matrix composition, and explant …

State Space Model Of A Light Weight Flexible Telescope, Chi-Yeh Hsu

Master's Theses

The accuracy of optical telescope in the past was dominated by the construction of the telescope body and the precision of optical surface. This thesis fits into a larger project with a goal of controlling a flexible telescope in order to help a cheaper telescope achieve higher accuracy. In order to understand the internal motion of telescope, the goal of this thesis is to build a state space model of a two-degree-of-freedom telescope using a known star location as feedback. The model presented in this thesis may be used for the telescope simulation and the control design in the future. …