Digital Commons Network^™

Modified Amino Acid Copolymers Suppress Myelin Basic Protein 85–99-Induced Encephalomyelitis In Humanized Mice Through Different Effects On T Cells, Zsolt Illés, Joel N.H. Stern, Jay Reddy, Hanspeter Waldner, Marcin P. Mycko, Celia F. Brosnan, Stephan Ellmerich, Daniel M. Altmann, Laura Santambrogio, Jack L. Strominger, Vijay K. Kuchroo

Jay Reddy Publications

A humanized mouse bearing the HLA-DR2 (DRA/DRB1*1501) pro- tein associated with multiple sclerosis (MS) and the myelin basic protein (MBP) 85–99-specific HLA-DR2-restricted T cell receptor from an MS patient has been used to examine the effectiveness of modified amino acid copolymers poly(F,Y,A,K)n and poly- (V,W,A,K)n in therapy of MBP 85–99-induced experimental auto-immune encephalomyelitis (EAE) in comparison to Copolymer 1 [Copaxone, poly(Y,E,A,K)n]. The copolymers were designed to optimize binding to HLA-DR2. Vaccination, prevention, and treatment of MBP-induced EAE in the humanized mice with copolymers FYAK and VWAK ameliorated EAE more effectively than Copolymer 1, reduced the number of pathological lesions, and …

Collaborative Management Of Hiv Infection In The Community: An Effort To Improve The Quality Of Hiv Care, Michael F. Parry, Julie G. Stewart, P. Wright, Gavin X. Mcleod

Nursing Faculty Publications

Our hospital led a multidisciplinary community team to improve the quality of care delivered to HIV-infected clients utilizing a disease management approach in a US metropolitan community of 150,000 people. Community needs assessment and client and community surveys were used to define the problems. Patient care flowcharting and the creation of an electronic patient database facilitated patient tracking across the entire community. Clinical guidelines and a consultation and referral immunology clinic standardized care practices. Measurable improvements in the quality of care were noted in multiple areas. Flowchart completion rates rose from 44% to 100%; medication adherence assessment rose from 82% …

Agribusiness Sheep Updates - 2004 Part 2, Anyou Liu, Clinton Revell, Phil Nichols, Brad Nutt, Darryl Clements, Lucy Anderton, Stephen Gherardi, Chris Oldham, Paul Sanford, John Gladman, G. E. Donald, A. Edirisinghe, D. A. Henry, S. P. Gittins, R. C. G. Smith, Roy Butler, Kelly Pearce, David Masters, David Pethick, Andrew Thompson, Ken Hart, Johan Greeff, Beth Paganoni, Rachel Kirby, Matt Ryan, Kira Butler, Roger Heggarty, David Hopkins, Samantha Giles, Tom Plaisted, Mark Ferguson, Darren Gordon, John Young, Sandra Brown, Ian Mcfarland, John Archer, John Milton, Rob Davidson, Graeme Martin, David Lindsay, Johnathan England, Mandy Curnow, Karina P. Wood, Ashley K. White, B. Lloyd Davies, Paul M. Carberry, Mark Hyder, Mike Freer, Andrew Van Burgel, Kazue Tanaka, Andrew Peterson, Roger Wiese, Gonzalo Mata, Evan Burt, Amanda Miller, Anne Bennett, Felicity Flugge, Amir Abadi, Perry Dolling, Dean Thomas, Mike Ewing, David Lindsay, Emma Kopke, E. A. Dowling, E. K. Crossley, Brien (Ben) E. Norton, John Karlsson, Geoff Pollott, Diana Fedorenko, Darryl Clements, Robert Beard, Brown Besier, Una Ryan, Caroline Bath

Sheep Updates

Precision Pastures

Using Species Diversity to Improve Pasture Performance Anyou Liu and Clinton Revell, Department of Agriculture, Western Australia

New Annual Pasture Legumes for Sheep Graziers Phil Nichols, Angelo Loi, Brad Nutt and Darryl McClements Department of Agriculture Western Australia

Pastures from Space – Can Satellite Estimates of Pasture Growth Rate be used to Increase Farm Profit? Lucy Anderton, Stephen Gherardi and Chris Oldham Department of Agriculture Western Australia

Summer-active Perennial Grasses for Profitable Sheep Production Paul Sanford and John Gladman, Department of Agriculture, Western Australia

Pastures From Space – Validation Of Predictions Of Pasture Growth Rates DONALD, G.E^{.A …}

Agribusiness Sheep Updates - 2004 - Part 1, Mark Dolling, Len Stephens, David Thomanson, Norm Adams, Shimim Liu, Peter Buchman, Mike Paton, Dianne Evans, John Edwards, Scott Champion, Alan Langford, A. C. Schlink, J. C. Greeff, M. E. Ladyman, Kimbal M. S. Curtis, John Stanton, Stuart Adams, Neal Fogarty, Matthew Kelly, James Skerritt, Ian Mcfarland

Sheep Updates

Proceedings of the Agribusiness Sheep Updates - 2004 Forward Dr Mark Dolling Manager, Sheep Industries and Pasture, Department of Agriculture Western Australia Keynotes Australian Wool Innovation Limited DR LEN STEPHENS AUSTRALIAN WOOL INNOVATION LIMITED (AWI) Commercialisation of Sheepmeat Eating Quality Outcomes, David Thomason, General Manger Marketing Meat & livestock Australia Limited PLENARY The Fitness of the Future Merino, Norm Adams and Shimin Liu, CSIRO Livestock Industries Ovine Johne’s Disease – Managing the Disease, Managing the Issues, PETER BUCKMAN, CHIEF VETERINARY OFFICER, DEPARTMENT OF AGRICULTURE WESTERN AUSTRALIA Animal Welfare – Changes in Latitudes Changes in Attitudes, Michael Paton and Dianne Evans, …

Risk Factors For Pre-Post Monsoon Cholera Epidemics In Bangladesh From 1992-1994, Rhonda Rae Robb

Dissertations and Theses

The primary objective of this thesis is to differentiate between the risk factors for pre-and post-monsoon cholera epidemics in rural Bangladesh by analyzing the complex interaction between select environmental, cultural/behavioral, and socioeconomic variables over space and time. In rural Bangladesh, cholera epidemics correspond with the annual monsoon: the first, and smallest, occurs between March and June, while the larger cholera peak occurs between September and December. The differences between the spatial and temporal patterns of seasonal cholera are analyzed, and the risk factors are calculated for pre-and post-monsoon cholera epidemics.

The theoretical approach that underlies this medical geographical study is …

Genetic Analysis Of The Putative Streptolysin O Regulator From Streptococcus Pyogenes, Robert Tyrone Billingslea

McCabe Thesis Collection

Group A streptococci (Streptococcus pyogenes) is one of the most frequent pathogens of humans. It is estimated that between 5-15% of normal individuals harbor the bacterium, usually in the upper respiratory tract, without signs of disease. S. pyogenes can initiate an active infection when defenses are compromised or when the organism is able to penetrate the host's defenses. When the bacteria invade vulnerable tissues, a variety of suppurative infections can occur. Acute S. pyogenes infection may be present as pharyngitis ("strep throat"), scarlet fever (rash), impetigo (infection of the superficial layers of the skin) or cellulitis (infection of the deep …

Impacts Of Sara And Agr In Staphylococcus Aureus Strain Newman On Fibronectin-Binding Protein A Gene Expression And Fibronectin Adherence Capacity In Vitro And In Experimental Infective Endocarditis, Yan-Qiong Xiong, Arnold S. Bayer, Michael R. Yeaman, Willem Van Wamel, Adhar C. Manna, Ambrose L. Cheung

Dartmouth Scholarship

We investigated the impacts of sarA and agr on fnbA expression and fibronectin-binding capacity in Staphylococcus aureus in vitro and in experimental endocarditis. Although sarA up-regulated and agr down-regulated both fnbA expression and fibronectin binding in vitro and in vivo, fnbA expression was positively regulated in the absence of both global regulators. Thus, additional regulatory loci contribute to fnbA regulation and fibronectin-binding capacities in S. aureus.

West Nile Virus; History Of The Virus And Implications Of Public Health For South Dakota In 2004, Candice Nebelsick

The Journal of Undergraduate Research

West Nile Virus is currently affecting human and animal life in North America. This Virus has been a popular topic among researchers, health professionals, and state health departments because of its rapid emergence as a threat to human and animal populations. In this article, I will be discussing the symptomology associated with West Nile Virus and the disease's pattern of transmission. Furthermore, I will describe procedures for diagnosing and treating West Nile Virus and minimizing its future spread into human populations. I will conclude this article by predicting how the West Nile Virus will impact South Dakotans in 2004.

Evaluation Of Immunogenicity Of Transgenic Chloroplast Derived Protect, Vijay Koya

Electronic Theses and Dissertations

Anthrax, a fatal bacterial infection is caused by Bacillus anthracis, a gram-positive, spore forming, capsulated, rod shaped organism. Centers for Disease Control (CDC) lists anthrax as Category A biological agent due to its severity of impact on human health, high mortality rate, acuteness of the disease and potential for delivery as a biological weapon. The currently available human vaccine in the United States (AVA anthrax vaccine adsorbed) is prepared from Alum adsorbed formalin treated supernatant culture of toxigenic, non-encapsulated strain of Bacillus anthracis with the principle component being protective antigen (PA83). Evaluation of anthrax vaccine given to nearly 400,000 US …

Modeling The Incubation Period Of Anthrax, Ron Brookmeyer, Elizabeth Johnson, Sarah Barry

Johns Hopkins University, Dept. of Biostatistics Working Papers

Models of the incubation period of anthrax are important to public health planners because they can be used to predict the delay before outbreaks are detected, the size of an outbreak and the duration of time that persons should remain on antibiotics to prevent disease. The difficulty is that there is little direct data about the incubation period in humans. The objective of this paper is to develop and apply models for the incubation period of anthrax. Mechanistic models that account for the biology of spore clearance and germination are developed based on a competing risks formulation. The models predict …

Socio-Economic And Diagnostic Aspects Of Tuberculosis In Pakistan, Saba Sohail

Department of Radiology

No abstract provided.

Sheep Updates 2003 - Husbandry, Paul Barrett, Kevin Bell, J Greeff, L. Butler, S. Brown, K. Hart, A. Gray, John Karlsson, Paula Coombe, Ron Mctarrart, David Scobie, Sandy White

Sheep Updates

This session covers seven papers from different authors:1. Setting up a successful, low input feedlot Paul Barrett, ‘Bimberdong’ Jerramungup 2. Effective mineral supplementation of sheep Kevin Bell, Sheep Management and Production Consultants, Kojonup, WA 3. Genetic benchmarking for WA sheep producers J. Greeff, L. Butler, S. Brown, K. Hart and A. Gray Department of Agriculture Western Australia 4. Does selecting sheep for low WEC reduce scouring? John Karlsson, Johan Greeff and Paula Coombe, Department of Agriculture Western Australia 5. Summer quarters for sheep - stubbles Ron McTaggart, Department of Agriculture Western Australia, Albany 6. Thinking about breeding Easy Care Sheep? …

Sheep Updates 2003 - Plenaries, Ashley Mercy, David Scobie, Neil Buchanan, Kevin Bell, John Milton, Graeme Martin, Bob Hall, Maurie Stephen, Rob Woodgate

Sheep Updates

This session covers eight papers from different authors:1. Biosecurity in the sheep industry will improve production and profits Ashley Mercy, Department of Agriculture, Western Australia 2. Easy care sheep David Scobie, AgResearch, PO Box 60 Lincoln, 8152, New Zealand 3. Targeting the market’s requirements - live sheep exports Neil Buchanan, Manager Animal Health Operations, Primary Industries & Resources, South Australia 4. Setting flock breeding objectives Kevin Bell, Sheep Management and Production Consultants, Kojonup WA 5. Future feeding of sheep in Western Australia John Milton and Graeme Martin, The University of Western Australia 6. Recipe for getting back into Sheep Bob …

Temporal Stability And Geographic Variation In Cumulative Case Fatality Rates And Average Doubling Times Of Sars Epidemics, Alison P. Galvani, Xiudong Lei, Nicholas P. Jewell

U.C. Berkeley Division of Biostatistics Working Paper Series

We analyze temporal stability and geographic trends in cumulative case fatality rates and average doubling times of severe acute respiratory syndrome (SARS). In part, we account for correlations between case fatality rates and doubling times through differences in control measures. We discuss factors that may alter future estimates of case fatality rates. We also discuss reasons for heterogeneity in doubling times among countries and the implications for the control of SARS in different countries and parameterization of epidemic models.

Regulation And Localization Of Endogenous Human Tristetraprolin, Anna-Marie Fairhurst, John E. Connolly, Katharine A Hintz, Nicolas J Goulding

Dartmouth Scholarship

Tumor necrosis factor (TNF) has been implicated in the development and pathogenicity of infectious diseases and autoimmune disorders, such as septic shock and arthritis. The zinc-finger protein tristetraprolin (TTP) has been identified as a major regulator of TNF biosynthesis. To define its intracellular location and examine its regulation of TNF, a quantitive intracellular staining assay specific for TTP was developed. We establish for the first time that in peripheral blood leukocytes, express

Outbreak Of Sars, Ambreen Shakil, Muhammad Imran Omar, Saba Sohail

Department of Radiology

No abstract provided.

The Ecological Ramifications Of Disease And Density In The Caribbean Spiny Lobster, Panulirus Argus, Donald C. Behringer Jr.

Biological Sciences Theses & Dissertations

In 1999, I discovered the first virus known to be pathogenic to any species of lobster. HLV-PA is a pathogenic herpes-like virus that infects juvenile Caribbean spiny lobster, Panulirus argus, in the waters off south Florida (USA), and it alters the behavior and ecology of this species in fundamental ways. Gross signs of HLV-PA infection are lethargy, morbidity, cessation of molting, and discolored, “milky” hemolymph that does not clot. HLV-PA infects the hemocytes of host lobsters, specifically the hyalinocytes and semi-granulocytes, but not the granulocytes. When hemolymph from infected donors was injected into healthy juvenile lobsters, 90% of the …

Enterobius Vermicularis: Ancient Dna From North And South American Human Coprolites, Alena M. Iñiguez, Karl J. Reinhard, Adauto Araújo, Luiz F. Ferreira, Ana Carolina P. Vicente

School of Natural Resources: Faculty Publications

A molecular paleoparasitological diagnostic approach was developed for Enterobius vermicularis. Ancient DNA was extracted from 27 coprolites from archaeological sites in Chile and USA. Enzymatic amplification of human mtDNA sequences confirmed the human origin. We designed primers specific to the E. vermicularis 5S ribosomal RNA spacer region and they allowed reproducible polymerase chain reaction identification of ancient material. We suggested that the paleoparasitological microscopic identification could accompany molecular diagnosis, which also opens the possibility of sequence analysis to understand parasite-host evolution.

Aids, Orphans, And Development In Sub-Saharan Africa: A Review Of The Dilemma Of Public Health And Development, Joe Lugalla

Institute for Educational Development, East Africa

HIV/AIDS is having devastating consequences on families, young children, and other vulnerable social groups. In this paper, I review the impact of HIV/AIDS on families and development in sub-Saharan Africa. I begin by showing the magnitude of the problem and the factors that have led to rapid spread of HIV/AIDS in this sub-continent. I discuss gender inequality, poverty, social inequality, and globalization, and show how these facilitate the rapid spread of the epidemic. I show how AIDS is creating a mass of orphans on the one hand, and how it is impacting development and creating new public health contradictions on …

Hemorrhagic Fever Viruses, Jeffery A. Goad, J Nguyen

Pharmacy Faculty Articles and Research

Viral hemorrhagic fever (VHF) viruses are diverse and include arenaviruses, bunyaviruses, filoviruses, and flaviviruses. Lassa fever, South American hemorrhagic fever (HF), Rift Valley HF, Crimean-Congo HF, Ebola, Marburg, yellow fever, and dengue fever are well known examples of the hemorrhagic fever viruses. They are often difficult to diagnose and treat resulting in significant morbidity and mortality. Transmission varies from vector borne to person-to-person. The common clinical presentation includes fever, myalgia, microvascular damage, and hemorrahage as well as a history of travel to the tropics. If VHF is suspected, strict infection control procedures must be implemented to prevent the spread of …

Hepatitis C, Quality Of Life And Cognitive Function : An Exploratory Study, John Caithness

Theses : Honours

Active chronic hepatitis C (CHC) is a viral infection that affects approximately 150,000 Austra1ians. It has various quality of life impacts and the literature suggests some cognitive ramifications. In this Western Australian exploratory study, 13 healthy students from Edith Cowan University made up a control group. One experimental group consisted of 11 people with CHC and mild liver damage, and a second experimental group consisted of 8 people with CHC and at least moderate liver damage. The participants were assessed with a health-related quality of life questionnaire, the Short Form 36 Health Survey (SF-36); a test of cognitive functioning, the …

Psychosocial Predictors Of Survival In Persons With Hiv Or Aids, Carlos A. Escoto

Loma Linda University Electronic Theses, Dissertations & Projects

The HIV pandemic is in its second decade of existence. HIV has distinguished itself by effecting every part of the globe. It is estimated that there are currently over 40 million people living with HIV or AIDS diagnoses in the world today. Great strides have been made in the treatment of HIV and AIDS. Combination therapy of drugs has been shown to keep viral replication at very low levels. The result of drug therapies has resulted in keeping persons with HIV from progression to full blown AIDS. Also, drug therapies are responsible for a significant drop in mortality rates from …

Supervised Treatment Interruption (Sti) In An Urban Hiv Clinical Practice: A Prospective Analysis, Joseph L. Yozviak Do, Facp, Peter Kouvatos Do, R Eric Doerfler Np, Cch, William C. Woodward Do

Department of Medicine

Background: In acute HIV-1 infection, STI may induce immunologic control of HIV-1 replication. Several prospective trials of STI in chronic HIV-1 infection have been less encouraging. A previously presented retrospective analysis of our patients showed that in those with a significant CD4 increase (>200 cells) on antiretroviral therapy (ART), 2 or more interruptions may significantly lower viral set point. This prospective study describes STI in a cohort of patients.

Methods: 10 patients with either a positive response to therapy interruption retrospectively or those expressing interest in the strategy who met inclusion criteria (VL BLQ on ART, good adherence, robust …

Human Exposure To Herpesvirus B–Seropositive Macaques, Bali, Indonesia, Gregory A. Engel, Lisa Jones-Engel, Michael A. Schillaci, Komang Gde Suaryana, Artha Putra, Agustin Fuentes, Richard Henkel

All Faculty Scholarship for the College of the Sciences

Herpesvirus B (Cercopithecine herpesvirus 1) has been implicated as the cause of approximately 40 cases of meningoencephalitis affecting persons in direct or indirect contact with laboratory macaques. However, the threat of herpesvirus B in nonlaboratory settings worldwide remains to be addressed. We investigated the potential for exposure to herpesvirus B in workers at a “monkey forest” (a temple that has become a tourist attraction because of its monkeys) in Bali, Indonesia. In July 2000, 105 workers at the Sangeh Monkey Forest in Central Bali were surveyed about contact with macaques (Macaca fascicularis). Nearly half of those interviewed had …

Miscellaneous Publication 6/2001 - Sheep And Goat Industries Biosecurity Plan, Department Of Agriculture Western Australia, Government Of Western Australia

Biosecurity published reports

StockGuard has been initiated to develop a series of biosecurity plans for the animal industries in Western Australia. Through StockGuard, representatives from the animal industries have been working with the Department of Agriculture to identify the major threats to each of the animal industries and to develop action plans detailing how these threats will be managed.

The recent Foot and Mouth outbreak in the United Kingdom is a stark example of the potential for exotic disease to devastate an animal industry. The UK experience, along with the 1999 outbreak of virulent Newcastle disease in poultry in New South Wales have …

Enhancement Of Anti-Hiv-1 Ribozyme Activities By Rev Binding And Multimerization, Yuksel Yildiz

Loma Linda University Electronic Theses, Dissertations & Projects

To effectively apply hammerhead ribozymes as therapeutic agents it is necessary to co-localize them with the desired target. Human immunodeficiency virus type1 (HIV- 1) infectivity is dependent on env gene expression. HIV-1 Rev protein binds to a higher ordered RNA structure within the env transcript termed the Rev Binding Element (RBE). In anti-HIV gene therapy employing ribozymes to increase the co-localization of anti- HIV ribozymes with target HIV mRNAs, it has been proposed that when the native HIV- 1 RBE is appended to a ribozyme as a decoy molecule, simultaneous binding of Rev monomers to the RBE sequences in both …

Characterization Of Host-Bacteria Interactions Contributing To Group B Streptococcus Colonization, Jennifer Marie Smith

Theses, Dissertations and Capstones

Group B streptococcus (GBS) is the leading cause of life-threatening bacterial infections during the first three months of life. GBS is also a frequent cause of maternal postpartum infections. Both types of infections stem from maternal vaginal and/or rectal colonization with GBS in the perinatal period. Limited information is available concerning how the colonization process occurs and what role the host immune system may play in the establishment of persistent colonization by GBS. The complex interactions between the immune system and GBS normally should end in clearance of the bacteria. However, since colonization by GBS occurs in a large number …

Effects Of Pre-Milking Teat Sanitation On The Quality Of Raw Milk, Laurie Depiazzi, Ian Bell

Bulletins 4000 -

The effect of Quartermate pre-milking teat sanitation on milk quality was investigated in twelve dairy herds over a period of eight weeks. Pre-milking teat sanitation was carried out on all cows for six weeks on each of six farms and mesophile and thermophile bacteria counts of raw milks were compared before the milk line (“cluster” samples), and at various points within milking plants. Rates of mastitis, vat milk iodide levels and specific bacteria were also monitored.

Preliminary Results From A Survey On The Prevalence Of Parasitic Helminths And Protozoa In Raccoons, Opossums, And Skunks, Michelle Belviy, T. A. Yazwinski, C. A. Tucker, Jennifer Robins

Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences

Raccoons, skunks, and opossums (N=57, 60, and 60, respectively) were necropsied for parasite detection and identification from September, 2001 until April, 2002. Qualitative coprological exams and adult Baylisascaris collections have been completed. Fecal stages and/or types found were Baylisascaris and Strongyloides-type (skunks and raccoons); Capillaria and Trichostrongyletype (raccoons and opossums); Acanthacephalan and ascarid type (opossums only); free larvae (skunks only); and coccidial (protozoan) oocysts (all three host species). Adult Baylisascaris were recovered from 33.3% of the raccoons and 58.3% of the skunks. Data collection relative to this survey, which is still ongoing, includes the determination of Sarcocystis prevalence in excised …

Health And Development: Infectious Intestinal Diseases In A Transborder Region (United States-Mexico), Flavia Bianchi

Theses and Dissertations (Comprehensive)

Intestinal infectious diseases are considered the northern Mexican border’s most obvious challenge facing children. The Mexico-US Border region is a 200-kilometer border zone that extends 100 kilometers on either side of an imaginary borderline and stretches 3,141 kilometers from the Pacific Ocean to the Gulf of Mexico. In 2000, the Pan American Health Organization (PAHO), the World Health Organization’s representative for the Americas, published a study of the top causes of mortality between 1995 and 1997 to estimate the absolute burden of mortality in the primary Mexican and American border community, collectively known as the Sister Cities. Using the PAHO …