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New Innovation In The Flipped Chemistry Classroom

The Graduate Review

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Parahydrogen Hyperpolarized Multi-Nuclear Contrast Agents For Potential Mri Applications, Nuwandi Maduranga Ariyasingha Uragoda Wiyannalage

Wayne State University Dissertations

ABSTRACT

PARAHYDROGEN HYPERPOLARIZED MULTI-NUCLEAR CONTRAST AGENTS FOR POTENTIAL MRI APPLICATIONS

by

NUWANDI M. ARIYASINGHA

December 2020

Advisor: Dr. Eduard Chekmenev

Major: Chemistry (Physical)

Degree: Doctor of Philosophy

There have been numerous studies to develop fast, cost effective hyperpolaried (HP) contrast agents with higher polarization values that can be potentially employed for magnetic resonance clinical imaging. Some of the currently used contrast agents are based on 129Xe hyperpolarized nucleus. However, one of the problems associated with this approach, is the high cost of the produced hyperpolarized contrast agents. In addition, the other important practical problem is that the currently used clinical …

Climate Change Instruction In Higher Education: Pre-Service Teachers’ Engagement In An Interdisciplinary Pop-Up Learning Community, Mary Charlotte Bautistia

Master's Theses and Doctoral Dissertations

An interdisciplinary pop-up learning community (PLC) allowed students from various disciplines with different levels of content knowledge to discuss their perspectives and beliefs on climate change. The impact of this event on students was gauged by a survey from the Yale Project on Climate Change Communication. Furthermore, this project focuses on pre-service teachers who participated in the PLC. In order to investigate how pre-service teachers understand climate change and how they may or may not integrate these issues into their own instruction, individual interviews were conducted prior to and after the PLC to determine if the event had an impact …

Biophysical And X-Ray Structural Studies Of The (Gggtt)3ggg G-Quadruplex In Complex With N-Methyl Mesoporphyrin Ix, Linda Yingqi Lin , '20, S. Mccarthy, Barrett M. Powell , '18, Yanti Manurung , '20, Irene M. Xiang , '18, W. L. Dean, B. Chaires, Liliya A. Yatsunyk

Chemistry & Biochemistry Faculty Works

The G-quadruplex (GQ) is a well-studied non-canonical DNA structure formed by G-rich sequences found at telomeres and gene promoters. Biological studies suggest that GQs may play roles in regulating gene expression, DNA replication, and DNA repair. Small molecule ligands were shown to alter GQ structure and stability and thereby serve as novel therapies, particularly against cancer. In this work, we investigate the interaction of a G-rich sequence, 5’-GGGTTGGGTTGGGTTGGG-3’ (T1), with a water-soluble porphyrin, N-methyl mesoporphyrin IX (NMM) via biophysical and X-ray crystallographic studies. UV-vis and fluorescence titrations, as well as a Job plot, revealed a 1:1 binding stoichiometry with an …

Convergent Synthesis Of Anaephene B, Mason A. Baber

Honors Program Theses

As strains of bacteria continuously evolve and become resistant to modern antibiotics, there exists a need for the development of new treatments to combat bacterial infection. Staphylococcus aureus (S. aureus) is known to develop such resistance and remains a leading cause of invasive infections and biofilm formation on prosthetic medical devices. In 2018, Brumley and Spencer et al. isolated anaephenes A–C, natural products from a marine bacterium. Anaephene B was of particular interest as it was the only member of the series to demonstrate moderate antibacterial activity against S. aureus (MIC = 6.1 μg/mL). Because of the potential clinical utility …

Exploring The Multiplex Detection Capabilities Of Raman Spectroscopy On Mock Street Samples Containing Illicitly Manufactured Fentanyls, Mia Laverne Williams Burnett

Browse all Theses and Dissertations

Illicitly manufactured fentanyls (IMFs) have become a global and local epidemic and have recently caused an increase in the number of accidental exposures and overdoses in first responders and the community. The aim of the research is to explore a method to discriminate between IMFs and cutting agents in mock street samples using Raman Spectroscopy. Predominant Raman bands corresponding to the structural moieties of each individual illicit, licit drugs, and cutting agents were tracked, then used to discriminate between varying components in a mock street sample. In conclusion, discrimination between the components in the mixture was obtained using Raman Spectroscopy. …

Clinical And Pharmaceutical Applications Of Affinity Ligands In Capillary Electrophoresis: A Review, Chenhua Zhang, Ashley G. Woolfork, Kyungah Suh, Susan Ovbude, Cong Bi, Marawan Elzoeiry, David S. Hage

David Hage Publications

Affinity capillary electrophoresis (ACE) is a separation technique that combines a biologically-related binding agent with the separating power and efficiency of capillary electrophoresis. This review will examine several classes of binding agents that have been used in ACE and applications that have been described for the resulting methods in clinical or pharmaceutical analysis. Binding agents that will be considered are antibodies, aptamers, lectins, serum proteins, carbohydrates, and enzymes. This review will also describe the various formats in which each type of binding agent has been used in CE, including both homogeneous and heterogeneous methods. Specific areas of applications that will …

A Brief Introduction To The Applications Of Small Organic Molecules As Food Additives, Zhou Zhou

Open Educational Resources

A new chapter in Introductory Organic Chemistry course. Lecture PowerPoint file and laboratory document are also included in this work.

• The goal of this chapter is to cover the basic ground of the food additives from the perspective of organic chemistry. This chapter has three parts.
  • Part one: Introduction of the food additive industry including the history, definition and the purposes of food additives.
  • Part two: The terminology in food additives and in organic chemistry, how to locate trustworthy data sheets for chemicals, and how to interpret the toxicity data.
  • Part three: Different families of organic compounds used as food …

Fluoresence Spectra Study Of Sterically Strained Isomers Of C24h14 Polycyclic Aromatic (Pahs) & Characterization Of A Bio-Sourced, Fluoresent, Ratiometric Ph Indicator With Alkaline Pka, Samar Abd Alelah

Electronic Theses and Dissertations, 2020-2023

This thesis is composed to two studies, each of which relies on recent advances in time-dependent density functional theory (TD-DFT) to accurately model fluorescence spectra. The first study focuses on evaluating the fluorescence spectrum of polycyclic aromatic hydrocarbons (PAHs) having a molecular weight of 302. Three isomers are analyzed including dibenzo[a,j]fluoranthene, dibenzo[a,k]fluoranthene and dibenzo[a,l]fluoranthene. These isomers differ in the placement of one of six aromatic rings and in the amount that each molecule deviates from planarity. The TD-DFT methods are used to compute spectra in an environment that simulates solvent (n-hexane). These computed spectra are compared to vibronically resolved experimental …

Synthesis, Characterization, Dna Binding, Topoisomerase Inhibition, And Apoptosis Induction Studies Of A Novel Cobalt(Iii) Complex With A Thiosemicarbazone Ligand, Stephen J. Beebe, Michael J. Celestine, Jimmie L. Bullock, Shayna Sandhaus, Jessa Faye Arca, Donald M. Cropek, Tekettay A. Ludvig, Sydney R. Foster, Jasmine S. Clark, Floyd A. Beckford, Criszcele M. Tano, Elizabeth A. Tonsel-White, Raj K. Gurung, Courtney E. Stankavich, Yuk-Ching Tse-Dinh, William L. Jarrett, Alvin A. Holder

Bioelectrics Publications

In this study, 9-anthraldehyde-N(4)-methylthiosemicarbazone (MeATSC) 1 and [Co(phen)₂(O₂CO)]Cl·6H₂O 2 (where phen = 1,10-phenanthroline) were synthesized. [Co(phen)₂(O₂CO)]Cl·6H₂O 2 was used to produce anhydrous [Co(phen)₂(H₂O)₂(NO₃)₃ 3. Subsequently, anhydrous [Co(phen)₂(H₂O)₂] (NO₃)₃ 3 was reacted with MeATSC 1 to produce [Co(phen)₂(MeATSC)](NO₃)₃·1.5H₂O·C₂ H₅OH 4. The ligand, MeATSC 1 and all complexes were characterized by elemental analysis, FT IR, UV–visible, and multinuclear NMR …

Stimuli-Responsive Gelators From Carbamoyl Sugar Derivatives And Their Responses To Metal Ions And Tetrabutylammonium Salts, Dan Wang, Anji Chen, Joedian Morris, Guijun Wang

Chemistry & Biochemistry Faculty Publications

Carbohydrate-based low molecular weight gelators (LMWGs) are interesting compounds with a variety of applications. In this research, a library of nineteen carbamate derivatives of N-acetyl-D-glucosamine were synthesized and characterized, and several derivatives were found to be effective LMWGs. They formed gels in pump oils as well as mixtures of water with ethanol or water with DMSO. The structures of the carbamoyl chains played an important role in the gelation properties, short chain aliphatic derivatives and phenyl carbamates formed gels in more solvents than certain aromatic and dimeric carbamates. The phenyl carbamate gelator was also selected for the encapsulation of …

Development Of Supported Pd-Based Catalysts For Carbon-Carbon Cross-Coupling And Oxidation Reactions, Mrinmoy Das

Theses and Dissertations

Heterogeneous catalysts are a crucial part of chemistry used for various chemical synthesis stages, pharmaceutical, and environmental applications. The use of nanoparticles or rather nanocatalysts enhances the heterogeneous catalytic applications. Both chemical and physical methods can be used to synthesize nanocatalysts. Chemical synthesis methods require comparatively fewer instruments and can easily synthesize a large amount of nanocatalysts. Nevertheless, chemical synthesis methods require reducing agents and solvents that can be toxic or harmful materials. They often need harsh reaction conditions as well, like high temperature and pressure. On the other hand, physical synthesis methods often require complex instrument setup, but their …

Discerning The Spectroscopic Properties And Chemical Reactivity Of Bioinspired Metallocomplexes, Ashley K. Forney

Theses and Dissertations

In Nature, metalloenzymes catalyze a variety of thermodynamically demanding reactions with remarkable performance under mild, physiological conditions. This innate ability is a driving force for the development of nature inspired, synthetic analogues that are robust and can catalyze a variety of organic transformations. This work involves the successful syntheses of four coordinate, bioinspired complexes, [M^II(n-DOB)] (M = Ni, Co, and Cu; n = 12 and 13), which have been characterized by NMR, ESI-MS, UV/Vis, EPR, and CV. Spectroscopic evidence presents the generation of reactive metal-(di)oxygen species that display amphoteric reaction behavior, making these rarities among inorganic oxidants. These …

Detection Of Biomolecules Via Ocp Measurements Using Npg Electrodes, Borkat Ullah

Theses and Dissertations

In sensing application nanoporous gold (NPG) has attracted much attention. NPG is prepared by dealloying Au:Ag alloys. Characterization was done using scanning electron microscopy (SEM), X-ray photoelectron spectroscopy (XPS), and cyclic voltammetry (CV). In this work, the open circuit potential (OCP) of ferricyanide, cysteine, uric acid, and ascorbic acid was measured in phosphate buffer solution (PBS) and biofouling solution containing bovine serum albumin (BSA) at pH 7.4. OCP measurement has been hindered due to biofouling and surface passivation on the metal electrodes. By the use of the NPG electrodes, electrochemical activity can be maintained. From the OCP measurement of cysteine, …

Solid Phase Extraction And High-Precision Mass Spectrometry Analysis Methods For Crassostrea Virginica Metabolome, Alyssa Darling

Theses and Dissertations

Metabolomics is the rapidly-growing field which focuses on the endpoints of biological processes, and has the capability to provide a snapshot of an organisms’ interaction with their environment on a molecular scale. The objective of the experiment herein was to develop a standard metabolic extraction technique coupled with ultrahigh-precision analytical tools to build a global metabolic pathway of the keystone species Crassostrea virginica. To achieve this, C. virginica whole-body samples were extracted using acetonitrile and methanol, then split into a dehydrated group and a diluted group. Following reversed-phase solid phase extraction (RP-SPE) of both groups, metabolites were identified using …

Solvolytic Lignocellulosic Biomass Liquefaction, Mustafa Alluhaibi

Electronic Theses and Dissertations

Increasing energy demand, petroleum prices, global warming, and depleting fossil fuel resources are the main challenges faced by the human beings. Many scientists are searching for sustainable and alternative sources of fossil fuels as solutions to these challenges. Lignocellulosic biomass is one of the renewable and eco-friendly abundant sources that has been considered by both academia and industry sectors as a renewable source for bio-oil and chemicals. Direct liquefaction of biomass in the sub-/super-critical solvent has considered a practical method to convert lignocellulosic material into liquid fuel. However, undesirable properties such as poor stability, low energy value, and high acidity …

Characterization Of Naturally Derived Polymer By Oxidative, Thermal, And Spectrometric Methods, Ranen Roy

Electronic Theses and Dissertations

Extensive use of fossil fuel for different purposes has resulted in significant depletion of it at an alarming rate. Therefore, a potential and sustainable alternative source are badly needed at this moment. Nowadays, lignocellulose biomass is appealing to much interest by the researchers because of its potential nature as a renewable carbon source. It is the most abundant renewable source which is composed of cellulose, hemicellulose, and lignin. Among them, lignin is considered the 2nd most abundantly natural polymer after cellulose which comprises 10-30 % of biomass depending on the source and the environment. Lignin is a three-dimensional cross-linked organic …

Antidiabetic Activity Of Cissus Rotundifolia Plant Growing In Saudi Arabia, Saad Ali Alshehri

Electronic Theses and Dissertations

Diabetes mellitus (DM) is a metabolic disease characterized by high levels of blood glucose resulting from defects in insulin production or action. A world-wide increase in the diabetes rate is projected to reach pandemic levels over the next 10-20 years. Current data shows that high numbers of the population in Saudi Arabia suffer from diabetes and prediabetes. Non-traditional treatments of diabetes from medicinal plants has shown potential antidiabetic activity. A considerable percentage of the population still prefer or use medicinal plants as a treatment or supplement to traditional medicine. Cissus rotundifolia (family Vitaceae) is a known plant in southwestern Saudi …

Implementing And Assessing The Use Of A New Strategy For Training Chemistry Graduate Teaching Assistants, Amanda Hyett

Electronic Theses and Dissertations

Graduate teaching assistants (GTAs) largely contribute to undergraduate education but are often underprepared for their role as educators. Most graduate students attend graduate school to perform research and are then asked to teach for the first time without sufficient pedagogy training. To assist in increasing the GTAs pedagogical knowledge, a scenario-based activity series was developed and implemented for first- and second-year GTAs. Developing scenario-based scenarios from actual laboratory events provided GTAs with the opportunity to practice prior to engaging in the laboratory classroom. The Teaching Assistant Intervention Activities (TAIAs) included topics such as interpersonal skills and behaviors, group process, working …

Scavenger Hunt: The Search For Sulfur Mustard-Neutralizing Compound In 2-Chloroethyl Ethyl Sulfide (Cees) Treated Human Keratinocyte (Hacat) Epithelial Cells, Tanner Diemer

Electronic Theses and Dissertations

Sulfur mustard is a highly toxic and dangerous vesicant that has been utilized as a chemical warfare agent (CWA) since World War I. Despite its extensive history, an effective antidote to sulfur mustard exposure still does not exist. With detectable levels of unreacted sulfur mustard lasting days after initial contact, a window of opportunity exists to administer a “scavenger” to affected individual immediately and in the days following exposure in hopes of reducing harm by neutralizing unreacted sulfur mustard. For this strategy to be effectively implemented, it is essential to identify a candidate compound with excellent affinity towards sulfur mustard …

The Characterization And Reactivity Of Tris(4-Methylpyrazolyl)Methane And Its Tungsten And Molybdenum Metal Complexes, Julia M. Pomeroy

Undergraduate Honors Thesis Collection

The scorpionate ligand tris(pyrazolyl)borate (Tp) has contributed to the understanding of coordination and organometallic chemistry and has proven to be useful in the synthesis of metalloprotein models. Studies focusing on the similar ligand, tris(pyrazolyl)methane (Tpm), have recently been made possible with improved synthetic techniques. Characterization of the Tpm ligand and its metal derivatives, [TpmW(CO)3L]+ and [TpmMo(CO)3L]+, unveiled unexpected activity in the 1H NMR chemical shift of the methane hydrogen after the coordination of a seventh ligand. The more electronegative the seventh ligand was, the more downfield the chemical shift appeared. Various analogs of Tpm have since been synthesized that substitute …

Non-Canonical Targets, Reaction Kinetics, And Cellular Potency Of Amino Acid-Linked Platinum(Ii) Compounds, Bett Kimutai

Wayne State University Dissertations

DNA serve as an ideal target where drugs such as cisplatin (cisPt) bind and exert their anticancer activities. CisPt is known to preferentially coordinate with DNA leading to formation of cisPt-deoxyguanosine (dGuo) adducts. The adducts distort the DNA structure and contribute to inhibition of DNA-mediated cellular functions and ultimately cancer cell death. Despite its utilization as an anticancer drug, cisPt has a number of drawbacks, which include toxic side effects and cellular resistance. Resistance occurs through processes such as repair of damaged DNA and inactivation of cisPt present in the cell. To overcome these challenges, the aim of this thesis …

Β-Methylumbelliferone Surface Modification And Permeability Investigations At Pentel™ Graphite Electrodes, Susan Warren, Brian Seddon, Ruth Pilkington, Alison Crossely, Philip Holdway, Eithne Dempsey

Articles

Electrochemical and micro-imaging analysis of a commercial graphite-composite material is presented following electro-oxidation with β-methylumbelliferone. Charge-transfer surface modification was observed for the graphite electrode, presumed to have arisen from adsorbed interfacial umbelliferone moieties. The molecular permeability of the new surface towards a range of similar, yet size-variable (23 Å3–136 Å3) molecular redox probes is discussed. Red-shift fluorescence in confocal microscopy offers further support for the presence of a surface-bound umbelliferone layer. An SEM-platinum profiling technique was used as an imaging tool to map the umbelliferone surface and size-distribution of electroactive sites.

K-Means And Hierarchical Cluster Analysis As Segmentation Algorithms Of Ftir Hyperspectral Images Collected From Cutaneous Tissue, Cassio Lima, Luciana Correa, Hugh Byrne, Denise Zezell

Conference papers

Fourier Transform Infrared (FTIR) spectroscopy is a rapid and label-free analytical technique whose potential as a diagnostic tool has been well demonstrated. The combination of spectroscopy and microscopy technologies enable wide-field scanning of a sample, providing a hyperspectral image with tens of thousands of spectra in a few minutes. In order to increase the information content of FTIR images, different clustering algorithms have been proposed as segmentation methods. However, systematic comparative tests of these techniques are still missing. Thus, the present paper aims to compare the ability of K-means Cluster Analysis (KMCA) and Hierarchical Cluster Analysis (HCA) as clustering algorithms …

A Comparative Study Of Drug Affinities Determined By Thermofluor And Kinetic Analysis, Oruba Shatnawi

Theses, Dissertations and Culminating Projects

Determining the binding affinity and potency in vitro is one of the significant steps that can give a clue for a new candidate drug during the drug discovery process. Thermofluor is a method used in measuring binding affinity (Kd) of protein-ligands interaction through determining the change in thermal denaturation temperature of protein using real time PCR (RT-PCR). Kinetic analysis assay is used to screen a library of compounds to calculate their potencies (IC50) and inhibition constants (Ki) and it can be performed by spectrophotometer technique. In this study, we used bovine carbonic anhydrase II (BCA II) enzyme, and four of …

Understanding The Rage Signaling Pathway And Its Contribution To Diabetic Complications, Leon Vegas Ho

Legacy Theses & Dissertations (2009 - 2024)

The binding of advanced glycation end products (AGEs) to the receptor for advanced glycation end products (RAGE) is an important feature of the RAGE signaling pathway that plays a role in the pathogenesis of diabetes. Under high glucose concentration, RAGE expression increases immensely from the formation of a Schiff base by glucose bounded to lysine. This triggers an inflammatory and immune response and upregulates the expression of RAGE and causes an accumulation of AGEs in the body. As a result, this leads to the development of diabetes and other complications such as, atherosclerosis, nephrothapy, and retinopathy. To remedy AGE accumulation, …

Novel Substrate-Free Cholinesterase Based Sensing Of Organophosphorus Compounds, Hailey J. Marini

Legacy Theses & Dissertations (2009 - 2024)

The increasing concerns of chemical weapons used by terrorists is growing, creating a need for infield detection methods that can rapidly detect these compounds that are a major health concern for civilians and military personnel. Displacement is a novel concept for the detection of cholinesterase inhibitors, including organophosphorus compounds, that could replace the classical instrumentation methods currently being used for diagnostics.

Identification And Analysis Of Body Fluid Traces For Forensic Purposes Using Vibrational Spectroscopy And Chemometrics, Ewelina M. Mistek-Morabito

Legacy Theses & Dissertations (2009 - 2024)

Recent years have seen a large expansion in the forensic field due to the introduction of novel analytical techniques. Body fluids are an extremely important type of evidence at crime scenes and the main source of DNA. Most current methods for the identification and examination of body fluid traces require chemical treatment and are therefore destructive to the sample. Thus, new methods that are nondestructive and provide rapid results with statistical confidence are desirable. Such methods could eliminate noninformative traces and allow investigators to focus on more valuable pieces of evidence. The ultimate goal of this research is to develop …

Novel Bioreactor Technology For Real Time In-Cell Nmr, Leonard Breindel

Legacy Theses & Dissertations (2009 - 2024)

Long has it been the goal of biochemists to observe a protein within the cellular environment allowing the true nature of a protein function to be determined within a cell. To understand the nature of a protein within a cell, the role of the cellular environment must be considered when determining the function as a protein. The cellular environment is a crowded place filled with macromolecules such as DNA, RNA and proteins that all interact with one another affecting the function of a protein. Intracellular macromolecules can reach concentrations of 300 g/L and occupy 30% of the available space in …

Towards Machine Learning In Chemical Sensing : Milk Differentiation And Quality Control Through Two-Dimensional Nano-Sensor Array, Yu Sheng Chen

Legacy Theses & Dissertations (2009 - 2024)

Herein, we developed a novel artificial tongue using machine learning and 12 nanoassemblies (2D-NAs) to identify and analyzed different kinds of milk beverages for quality control. This biomimetic sensor array was trained to “taste” different milk types as an “artificial tongue” which is the first time we demonstrated that this sensor array can be implemented to complex systems. Two-dimensional nanoparticles (2D-nps) and nine fluorescently labeled single stranded oligonucleotides (ssDNA) with different length and nucleobases were assembled to create 12 2D-NAs. The artificial tongue was deployed to identify and analyze five milk types. All five milk types were discriminated with 95% …