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Inhibition Of N⁸-Acetylspermidine Deacetylation Activity Of Rat Liver, Michael J. Santacroce

University of the Pacific Theses and Dissertations

An enzyme activity located in the soluble fraction of rat liver which is known to deacetylate N⁸-acetylspermidine is studied. Using a crude cytosol preparation from rat liver, tritium-labelled N⁸-acetylspermidine [acetyl-³H] was shown to undergo deacetylation which was dependent upon both time of incubation and protein concentration. The Michalis constant for this deacetylation of N⁸-acetylspermidine is approximately 4.4 μM.

N¹-Acetylspermidine showed a competitive inhibition of this N⁸-acetylspermidine deacetylation activity, and was found to be the most potent inhibitor tested. Diamine and polyamine compounds were also shown to inhibit the …

The Association Of 5.8 S With 28s Ribosomal Rna, Nandita Banerjee

Chemistry & Biochemistry Theses & Dissertations

5.8S rRNA is a low molecular weight ribosomal RNA which is noncovalently bonded to the larger ribosomal subunit 28S rRNA; through its 3' end and through its 5' end. This interaction is an integral part of the ribosome, and plays an important role in the ribosome structure and function.

There is a high degree of homology between the 5.8S rRNA primary structures of rat, turtle and chicken. The base sequence of rat 5.8S rRNA differs only in one position from that of turtle and in three positions fr.om that of cl1.icken. Tl1ere is a single purine substitution at the 5' …

The Synthesis Of (3s-8as)-3-Benzyl-1-Oxo-Hexahydro-2h-Pyrrolo (1,2-A)Pyrazine, Judy Kay Gantenbein

Dissertations and Theses @ UNI

The synthesis of (3S-8aS)-3-benzyl-1-oxo-hexahydro-2Hpyrrolo(1,2-a) pyrazine was desired for further experimentation on its metabolic effects on day old cockerels. This compound was synthesized using available compounds of L-phenylalanine and L-proline. The intermediate compounds synthesized were L-phenylalanine methyl ester hydrochloride , L-phenylalinol, N-carbobenzoxy1-proline, p-nitrophenyl ester of N-carbobenzoxy-L-proline , N-carbobenzoxy-L-prolyl-L-phenylalainol, N-carbobenzoxy-L-prolylL-phenylalinyl chloride , and L- prolyl-L-phenylalinyl chloride. A discussion of methods of peptide linkage with specific emphasis on the carbobenzoxy N-protecting group and the methods for activating the carboxylic acid of the N-protected amino acid is included. Also discussed are the method and problems encountered in the removal of the carbobenzoxy group from …

Mobility Of Solvent Molecules In A Nonaqueous Lyotropic Liquid Crystal, David W. Larsen, Stig Friberg, Hugo Christenson

Chemistry Faculty Research & Creative Works

No abstract provided.

An Investigation Of Reversed Phase Thin-Layer Chromatography For The Separation Of Polycyclic Aromatic Hydrocarbons, Marvin V. Gibson

Dissertations and Theses @ UNI

A reversed phase thin-layer chromatographic method employing the chemically bonded octadecylsilane as the stationary phase (Whatman KC18), has been investigated for the simultaneous analysis of polycyclic aromatic hydrocarbons (PAH) and nitrogen heterocyclics (aza-arenes). Separations of mixtures of reference compounds were obtained with a number of mobile phases (developers) and the separation efficiencies depending on the individual solvent components were summarized. Double development and twodimensional thin-layer chromatography were used for identification purposes involving several environmental samples. This method, coupled with selective excitation fluorescence spectrometry, proved to be effective for the quantitative measurements of selected PAH and aza-arene compounds. The analytical procedures …

Surface Area Of Coal As Influenced By Low Temperature Oxidation Processes, Salvador Leon Arredondo

Dissertations and Theses

The possibility of increasing the amount of readily accessible surface area by enlarging the 5 A pore was examined via pretreating the PSOC-371 coal with gases such as nitrogen, nitrogen-oxygen, and ozone-oxygen and hydrogen peroxide solutions. Surface areas were obtained from nitrogen adsorption at 77 K and carbon dioxide adsorption at either 298 or 195 K for each sample before and after treatment.

Evidence For An Iminium Cation Intermediate And Its Non‐Oxidative Decarboxylation In A Cerium (Iv)‐Mediated Oxidative Decarboxylation. Preliminary Communication, Samir B. Hanna, Marilyn E. Moehlenkamp

Chemistry Faculty Research & Creative Works

Evidence is provided for the occurrence of a non‐oxidative decarboxylation in an iminium cation intermediate which is produced in an oxidative decarboxylation induced by cerium (IV). Copyright © 1980 Verlag GmbH & Co. KGaA, Weinheim

Spectroscopic Investigation Of The Mutual Interactions Between Nonionic Surfactant, Hydrocarbon, And Water, Hugo Christenson, Stig Friberg

Chemistry Faculty Research & Creative Works

NMR, UV, and IR spectroscopy were used to determine the interactions in hydrocarbon solutions between polyethylene glycol alkyl ethers, water, and aromatic hydrocarbons. The results showed a concentration dependence for the site of benzene in benzene/surfactant solutions. At low concentrations the benzene molecules were preferentially located adjacent to the surfactant oxyethylene chain with an even distribution along the chain. At high benzene concentrations a positive concentration gradient was found toward the hydrocarbon end of the oxyethylene chain. Addition of water increased this gradient except for the octa ethylene glycol dodecyl ether; for this compound the preferential water location appeared to …

Nmr Investigations Of Aggregation Of Nonionic Surfactants In A Hydrocarbon Medium, Hugo Christenson, Stig Friberg, David W. Larsen

Chemistry Faculty Research & Creative Works

Proton NMR, carabon-13 NMR, light-scattering, and density measurements were used to investigate association structures and molecular interactions during water solubilization in polyoxymethylene dodecyl ether/benzene systems at high surfactant concentrations. The results indicated an absence of association aggregates at low water concentrations. At water contents between 10 and 20%, association structures started to form, probably micelles of the inverse type, in which the water-polyoxymethylene chain interaction is the predominant feature. There is evidence that the average chain conformation changes with water content, and that bound water molecules are distributed evenly along the polyethoxyethylene chain. At higher water concentration, the results indicated …

Analysis Of Mixtures Of Chlorine, Chlorine Dioxide And Chlorite, Ying-Chien Chu

Masters Theses

No abstract provided.

Kinetic Study Of The Formation Of Mixed Ligand Complexes Formed From Triethylenetetramine Nickel (Ii) And Bidentate Ligands, Burtrand Insung Lee

Masters Theses

No abstract provided.

The Solid State 13C-Nmr And 19F-Nmr Spectra Of Some Graphite Fluorides, Charles A. Wilkie, Gong-Yu Lin, Daniel T. Haworth

Chemistry Faculty Research and Publications

The solid state ¹³C nuclear magnetic resonance spectra of fluorinated graphites show two resonances, one of which is assigned to aromatic carbon and the other to aliphatic carbon. The resonances are very broad with the high-field resonance centered at about 35 ppm below tetramethylsilane (TMS) and a low-field resonance centered at about 160 ppm below tetramethylsilane. The high-field resonance is typical of an sp³-like carbon and the low-field resonance is assigned to sp²-like carbons. It is found that the aromatic resonance in graphite decreases with an increase in fluorination of the graphite fluorides examined in …

Nonenzymatic Glucosylation Of Rat Albumin: Studies In Vitro And In Vivo, James F. Day, Robert W. Thornburg, Suzanne R. Thorpe, John W. Baynes

Faculty Publications

Incubation of rat serum with D-glucose in vitro resulted in nonenzymatic glucosylation of serum proteins. Analysis of freshly isolated rat albumin by ion exchange chromatography indicated that the glucosylated albumin accounts for 6.7.+-. 0.9% of total albumin in normal rat serum. Glucosylation of rat albumin in vitro was 1st order with respect to glucose and albumin concentrations and occurs primarily (> 90%) at intrachain lysine residues. Kinetic analysis and inhibition of glucosylation by aspirin suggest that 1 reactive lysine residue is the primary site of glucosylation. Less than 5% of the radioactivity from glucosyl-albumin was released as glucose or mannose …

Flat-Band Potential Of N-Type Semiconducting Molybdenum Disulfide By Cyclic Voltammetry Of Two-Electron Reductants: Interface Energetics And The Sustained Photooxidation Of Chloride, Lynn Schneemeyer, Mark S. Wrighton

Department of Chemistry and Biochemistry Faculty Scholarship and Creative Works

Cyclic voltammetry has been used to locate the band edges of n-type MoS2 in CH3CN/ and EtOH/[n-Bu4N]ClO4 solutions. The crucial experiments concern the study of the cyclic voltammetry of biferrocene (BF) and N,N,N',N'-tetra-methyl-p-phenylenediamine (TMPD) each of which has two, reversible, one-electron waves at Pt. At MoS2, the first oxidation is reversible in the dark, whereas the second oxidation is observed only upon illumination of the MoS2. The dark oxidation BF → BF+ and the photoanodic BF+ → BF2+ are separated by only ~150 mV, allowing us to assign an uncommonly accurate flat-band potential of +0.30 + 0.05 V vs. SCE …

Enhanced Nonenzymatic Glucosylation Of Human Serum Albumin In Diabetes Mellitus, C. Earl Guthrow, Mary Ann Morris, James F. Day, Suzanne R. Thorpe, John W. Baynes

Faculty Publications

Use of an ion exchange chromatographic method and a colorimetric method with thiobarbituric acid showed that levels of nonenzymatically glucosylated serum albumin were increased in patients with poorly controlled diabetes mellitus compared to controls. The two methods correlated well (r = 0.99) and clearly discriminated between normal and poorly controlled diabetic populations. The levels of glycosylated hemoglobin were also measured in both populations. Several patients apparently in good control based on glycosylated hemoglobin measurements were found to have increased levels of glycosylated albumin. Because albumin has a shorter circulating half-life than does the human erythrocyte, the plasma concentration of glucosylated …

Heating-Effect Minimization In Dye Lasers, Kenneth Lee Matheson, James M. Thorne

Faculty Publications

Optical pumping of dye lasers deposits heat nonuniformly in the active medium. Because the refractive index of a liquid or gas is a function of temperature, gradients are created which refract the laser beam. This effect is sometimes serious enough to quench laser action. In this paper, we evaluate the effects caused by optical pumping with a coaxial flashlamp and suggest ultraviolet absorbers and wavelength shifters as materials for minimizing the problem

Preparation And Properties Of Benzotropones And Benzohomotropones, Robert E. Suder

Dissertations

No abstract provided.

21st Rocky Mountain Conference On Analytical Chemistry

Rocky Mountain Conference on Magnetic Resonance

Abstracts and meeting program from the 21st annual meeting of the Rocky Mountain Conference on Analytical Chemistry, co-sponsored by the Rocky Mountain Section of the Society for Applied Spectroscopy and the Rocky Mountain Chromatography Discussion Group. Held in Denver, Colorado, July 30 - August 1, 1979.

Synthesis And Characterization Of Bis(Fulva1ene)- Divanadium And The Crystal Structure Of Its Oxidation Product, Bis(Fulvalene)Bis(Acetonitrile)Divanadium(Iii)- (V-V) Bis(Hexafluorophosphate)-Acetonitrile (1/1), James Smart, Barry Pinsky, Michael Fredrich, Victor Day

Department of Chemistry: Faculty Publications

Because of the current interest in the structure and reactivity of coordinatively unsaturated metallocenes and the formally analogous metallofulvalene derivatives, we wish to report our initial synthetic, physical, and X-ray structural studies of the bis( fulva1ene)divanadium system.

Isomerization And Dehydrocyclization Of 1,3-Pentadiene, Thomas E. Marcinkowski

Retrospective Theses and Dissertations

Piperylene concentrate is a complex mixture of 5-carbon unsaturated hydrocarbons obtained as a by-product when naphtha or gas oils are cracked. The major component in this mixture is 1,3-pentadiene. During the course of this study, a number of trials, utilizing liquid phase reaction conditions, were made to investigate the geometric isomerization of 1,3-pentadiene and its separation from the piperylene concentrate. Isomerization was accomplished employing catalytic amounts of iodine at temperatures ranging from 0°C to reflux. Using this method, the maximum amount of trans-1,3-pentadiene obtained was 70% as compared to 51% in the piperylene concentrate. Recovery of the product was 90%, …

Comment On "Depolarized Rayleigh Spectroscopy From Benzonitrile", Scott Whittenburg

Chemistry Faculty Publications

No abstract provided.

Thermal Effects On The Optical Spectra Of A Polydiacetylene: Poly{1,2-Bis [4-(Isopropylcarbamoyloxy) -N-Butyl]-1-Buten-3-Ynylene}, H. Eckhardt, Craig J. Eckhardt, K.C. Yee

Craig J. Eckhardt Publications

The reflection spectra of single crystals of the polydiacetylene, IPUDO [R= (CH₂)₄OCONHCH (CH₃)₂], were measured between 25° C and 160° C. A nearly reversible thermochromic change is observed together with a variation of hysteresis upon cycling. The latter is attributed to annealing that permits the pendant groups to adopt a conformation which favors the butatriene-type configuration of the polymer at high temperatures. Intensities of the high and low temperature spectra obtained by Kramers–Kronig transforms are in excellent agreement with the spectral intensities of polydiacetylenes which are prototypes for the two configurations.

Crystal Structures Of A₂Fecl₅·H₂O (A=Rb⁺, Cs⁺) And Field Dependent Superconducting Susceptometer Measurements, Charles J. O'Connor, Bascom Sine Deaver, Ekkehard Sinn

Chemistry Faculty Research & Creative Works

Crystal structures and low field principal crystal axis magnetic measurements are reported for the antiferromagnetic iron (III) salts A₂FeCl₅·H₂O; A=Rb⁺, Cs⁺. The two compounds undergo a zero field paramagnetic to antiferromagnetic phase transition at 6.5 K (A=Cs⁺) and 10.05 K (A=Rb⁺). The spin flop magnetic phase is observed in high field measurements. The magnetic field-temperature phase diagrams are reported. All magnetic measurements were recorded on a superconducting susceptometer. The bicritical points are 6.25 K, 14.5 kOe (A=Cs⁺), 9.75 K, 17.8 KOe (A=Rb⁺). …

The Concentration And Speciation Of Sugars In Natural Waters, Minoo Shakerin Sweet

Dissertations and Theses

Due to the importance of carbohydrates in biological systems, many efforts have been made to develop a quantitative method for analysis of carbohydrates in natural waters. The low concentrations of dissolved sugars in natural waters require a sensitive analytical method. In this study, gas chromatography of alditol acetate derivatives of sugars was investigated for quantitative and qualitative analysis of individual dissolved sugars in natural waters. The alditol acetate derivatives of sugars give only one derivative for each sugar, yielding qualitative and quantitative results.

The detection limit was 25 nM for each sugar. Because of this very low detection limit, only …

Surface Tensions Of Molten Salt Mixtures, Jerry Goodisman

Chemistry - All Scholarship

It is always found that the surface tension of a mixture of molten salts lies below the mean of the surface tensions of the pure components, weighted by mole fractions. Part of this effect is certainly due to the well-known fact that the composition near the surface is enriched in the component of lower surface tension. We show that an effect of the long-range forces, present for fluids involving Coulombic interactions, is of greater importance. The effect is due to the electroneutrality constraint on the distribution functions. As we show by separate calculations, both effects must be considered to obtain …

Analytical Applications Of Ultrasonic Velocity Using Three-Transducer Phase-Shift Detection, Chuan Chen

Chemistry and Chemical Biology ETDs

A new method for determining ultrasonic velocity and its appli­cation as a high-performance liquid chromatography (H.P.L.C.) detector is developed. The method involves in using a three­transducer phase-shift device where two transducers alternately transmit 1-MHz continuous wave signals to the same receiver. Ultra­sonic velocity can be calculated from the phase-shift difference between the two cell paths. When the device is used as an H.P.L.C. detector, the reciprocal of the phase-shift difference between the two cell paths is converted to an electrical signal and recorded on a chart paper with respect to retention time. The theoretical principles are discussed and the appropriate …

The Effects Of Bridging Ligands On Anodic Stripping Voltammetric Analysis, Larry Beasley

Masters Theses & Specialist Projects

Anodic stripping voltammetry (ASV) is an electroanalytical method used in the determination of trace metals in solutions volumes of 20 milliliters or less. Generally, 10^-6 M concentrations are routinely analyzed.

The analysis of Zn(II), Cd(II), and Cr(III) by ASV was Investigated, and the effects of introducing the bridging ligands, cyanide and thiocyanate into solutions of these metal ions was examined. These effects include a cathodic shift in the reduction potentials of the metals, and ASV signal changes ranging from small increases in peak currents to a total loss of peak-current signals, depending on the concentration of the ligand added.

Site Specificity In The Intercalation Of Antitumor Antibiotics With Dna, Martin Bradford Jones

Chemistry and Chemical Biology ETDs

Many planar molecules or molecules with planar moieties have been shown to bind to DNA by intercalation, which can be defined as the insertion of a planar moiety between adjacent bases on the DNA strand, reading in the 3'-5' direction, accompanied by a partial unwinding and elongation of the helix in the immediate vicinity of the intercalation site. Intercalation raises the question of specificity of binding of the drug to DNA, since there are 16 possible intercalation sites in normal double-stranded DNA. The conformational changes (elongation and unwinding) associated with intercalation make circular dichroism (CD) an excellent technique for studying …

Bulletin 3992 - Nitrogenous Fertilisers For Cereal Production, M. G. Mason

Bulletins - 3000 - 3999

Because fertiliser prices continue to rise, it is becoming increasingly important to understand what affects the response to fertilisers by crops.

Among the many factors influencing crop response to nitrogen applications, the most important are the season, soil type, paddock history, time of application and source of nitrogen. Before deciding whether to use nitrogen on a cereal crop, which source to use and at what rate, the farmer needs some understanding of how these factors work.

Kinetic Studies Of Polymer-Bound Rhodium (I) Hydrogenation Catalyst, Nee-Yin Chou

Masters Theses & Specialist Projects

For many years, it has been customary to classify catalysts as homogeneous or heterogeneous. Generally, the former are more active and more selective. But the major disadvantage of the homogeneous catalysts is the need to separate the reaction products and to recover the catalyst, which is often somewhat more expensive than a "classical" heterogeneous catalyst. It has been considered, especially in the last ten years, that there might be advantages in chemically binding a homogeneous catalyst to a solid support.

Polymer-bound, anthranilic acid anchored rhodium (I) catalysts have been prepared. The rate of hydrogenation of cyclohexene has been studied quantitatively, …