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Simultaneous Determination Of 3-Methoxy-4-Hydroxyphenyl-Glycol, Homovanillic Acid, And 5-Hydroxyindoleacetic Acid In Plasma By High Performance Liquid Chromatography, Yung-Te Tseng

Chemistry & Biochemistry Theses & Dissertations

A rapid, reliable, and sensitive method for simultaneous determination of 3-methoxy-4-hydroxyphenylglycol (MHPG), Homovanillic acid (HVA), and 5-hydroxyindoleacetic acid (5- HIAA) in plasma is described. The method is based on high performance liquid chromatography with electrochemical detection. Samples were filtered with Amicon Centrifree micropartition systems and extracted with organic solvent before chromatography. Chromatographic separation of sample components was achieved isocratically in a C18 reverse-phase column. Quantitation was performed by measuring the peak height ratios of the sample components to an internal standard. Analytical recoveries ranged from 50.78 (SD 4.2%) to 67.94 (SD 3.28). Within-run coefficient of variations (CVs) ranged from 3.78 …

Paleogeography And Paleoenvironments Of The Lower Unit, Fossil Butte Member, Eocene Green River Formation, Southwestern Wyoming, Roberto Enrique Biaggi

Loma Linda University Electronic Theses, Dissertations & Projects

During Eocene time sediment accumulated in Fossil Lake, in what developed to be a small linear and structurally controlled basin. Fossil Lake was one of several lakes into which the Green River Formation was deposited in Wyoming, Utah and Colorado.

Detailed stratigraphic analysis of the Lower Unit of the Fossil Butte Member revealed a well developed lacustrine sequence south of Fossil Butte, and indicates four major depositional facies: (1) open lacustrine, (2) marginal lacustrine, (3) carbonate mudflat, and (4) marginal fluvio-deltaic. The open lacustrine facies is characterized by kerogen rich to kerogen poor finely laminated micrites , that consist mainly …

Synthesis Of Highly-Strained Gem-Difluoro Compounds By Reaction Of Difluoromethylene With Cycloalkenes, Deyi Yang

Theses

Trans-Cyclooctene is thought to be more reactive than cis-cyclooctene due to its trans-structure. When cis or trans-clooctene reacts with difluormethylene (:CF₂), trans-9,9-difluorobicyclo[ 6,1,0] nonane was generated in much higher yield than cis-9,9-difluorobicyclo[6,1,0]nonane. [PhCF₃P⁺CF₂Br]Br^- was chosen to be the difluoromethylene precursor in this study. The reason was that the reaction condition for trans-cyclooctene and :CF₂ should be at low temperature. Only [PhCF₃P⁺CF₂Br]Br^- could generate CF₂ at lower temperature (e.g. room temperature). We succeeded in the synthesis of trans-9,9-difluorobicyclo[6,1,0]nonane and its cis isomer and …

Substituent Effects On Strained Rings. Part A ; The Kinetics Of Hydrolysis Of A Quinuclidone Using Ftir. Part B, Yeu-Yi Chiu

Theses

PART A: The energies of a series of monosubstitued propane, cyclopropane and cyclopropene compounds were calculated using ab initio molecular orbital calculations optimized at the 3-21G level using Gaussian 82 and Gaussian 86 programs. The effects of substituents on stabilization energies and geometries are rationalized with respect to the parent molecules. The result is presented which indicated that for substituted cyclopropyl and cyclopropenyl compounds, most of the substituents induce stabilization except the —NC substituent group is destabilizing in cyclopropyl and cyclopropenyl systems. Indications of relative electrostatic and resonance effects are also analyzed. A linear free energy relationship has been exam-ined …

Determination Of Benzo(A)Pyrene Metabolites In The Bile Of Winter Flounder From The New York Bight, Xingmin Liu

Theses

A method for detecting the concentration of BaP Metabolites in fish tissues has been developed. The analytical procedure includes extraction of PAH and BaP Metabolites from fish bile, liver extract and muscle extract; reduction of metabolites to BaP using hydriodic acid; and subsequent analysis of the BaP by thin layer chromatography with fluorescence detection. The method is applied to tissues from fish collected from polluted as well as clean reference waters and experimental tanks with dilutions of treated industrial wastewater. Experimental results on one kind of bottom-dwelling fish, winter flounder, which comes from the Hudson-Raritan Estuary, NY, where the water …

Synthesis Of Site Specific 1, N^2-Ethenodeoxyguanosine In A Small Oligonucleotide, Kathryn A. Carolin

Wayne State University Theses

Malondiadehyde (MDA) is a known carcinogen and mutagen produced by lipid peroxidation and prostaglandin synthesis in vivo. MDA is unique from structurally related compounds, methyl glyoxal and acrolein, because it produces frameshift mutations whereas the latter compounds induce base-pair substitutions. MDA forms several adducts with deoxyguanosine but it is not certain which one causes the frameshift mutations. Ideally, a single adduct on a replicating genome could simulate the in vivo process by which MDA mutagenesis would occur. This technique is known as site-specific mutagenesis. The approach of choice for site-specific mutagenesis experiment is the gapped heteroduplex method which requires the …

Origins Of The Quantum Efficiency Duality In The Primary Photochemical Event Of Bacteriorhodopsin, Robert R. Birge, Leonore A. Findsen, Albert F. Lawrence, Mark Masthay, Chian-Fan Zhang

Chemistry Faculty Publications

Experimental and theoretical evidence is presented which suggests that two distinct forms of light-adapted bacteriorhodopsin may exist. We propose that these two forms have characteristic photocycles with significantly different primary quantum yields. INDO-PSDCI molecular orbital procedures and semiempirical molecular dynamics simulations predict that one ground state geometry of bR undergoes photochemistry with a primary quantum yield, Φ1, of ~ 0.27, and that a second ground state geometry, with a slightly displaced counterion, yields Φ1 ~ 0.74. This theoretical model is supported by the observation that literature measurements of Φ1 tend to fall into one of two categories- those that observe …

Assignment Of The Raman Active Lattice Vibrations In Various Phases Of 1,2,4,5 Tetrabromobenzene And 1,2,4,5-Tetrachlorobenzene Crystals, K.M. White, Craig J. Eckhardt

Craig J. Eckhardt Publications

Results of polarized Raman spectroscopy are reported and used to obtain complete assignment of the optical lattice modes of 1,2,4,5-tetrabromobenzene crystals for both the beta and gamma phases. Similar information is provided for the beta phase of 1,2,4,5-tetrachlorobenzene crystal. The results are compared with lattice-dynamics calculations. Discrepancies with previously reported measurements and calculations are discussed and rationalized. The Journal of Chemical Physics is copyrighted by The American Institute of Physics.

A Study Of Denitrosation Of N-Nitroso Compounds By Irradiation With Long-Wavelength Uv Light, Zhenyu J. Wang

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

N-Nitrosamines and N-nitrosamides have been reported to be mutagenic. N-Nitrosamides are direct mutagens that need no activation to be mutagenic, whereas N-nitrosamines need to be enzymatically activated to exert their mutagenicity. Oxidative demethylation of nitrosamines is a commonly accepted activation mechanism. Another pathway of nitroso compounds, denitrosation, has recently been proposed. The mechanism of denitrosation, however, is still unknown.

The purpose of this study was to use a photo -reaction model to explore the possible denitrosation mechanism of N-nitroso compounds. An N-nitrosamine, N-nitrosomorpholi ne (NMOR), and an N-nitrosamide, N-methyl-N'-nitrosoguanidine (MMNG), were irradiated with long-wavelength UV light in the presence of …

Total Maximum Daily Loads And Waste Load Allocations For Las Vegas Bay: Rationale And Calulations, State Of Nevada: Division Of Environmental Protection

Publications (WR)

In September 1987 the Nevada Division of Environmental Protection revised Water Quality Standards for Las Vegas Wash (LVW) and Lake Mead. Standards were set for chlorophyll-a and unionized ammonia in Las Vegas Bay (LVB) (NDEP 1987). The 1986 and 1987 LVB data showed non-achievement of both the chlorophyll-a and unionized ammonia standard which triggered an analysis of total phosphorus and total ammonia Total Maximum Daily Loads and Waste Load Allocations which are presented in this report. This report is separated into three sections: 1. 1987 Las Vegas Bay Water Quality Conditions and Seasonal Periodicity. 2. Concentration Estimates at Northshore Road …

On The Kelvin Electrostatic Generator, S. Desmet, F. Orban, Fernande Grandjean

Chemistry Faculty Research & Creative Works

The Kelvin generator is an amazing electrostatic device which poses many questions. The authors report the results of their investigations of this device under various controlled conditions with both nonpolar and polar liquids and a sodium chloride aqueous solution. They have found that the generator works well even if the two liquid streams originate from different electrically insulated reservoirs. In addition they propose a model in which the electric charge results from the separation of the hydrogen and hydroxyl ions as the water droplets form.

The Spectroscopy And A State Dynamics Of The Neibr Van Der Waals Complex, William Robert Simpson , '88, Thomas Alex Stephenson

Chemistry & Biochemistry Faculty Works

The A  3Π1←X  1Σ+ laser‐induced fluorescence excitation spectrum of the NeIBr van der Waals complex is reported and analyzed to extract information regarding the structure and vibrational predissociation dynamics of the complex. While no definitive geometric information regarding NeIBr is obtained, our data indicate that a linear geometry is at least plausible. The vibrational predissociation lifetimes are a strong function of A state vibrational level and range from 2.6 to 23 ps. The variation in lifetime with vibrational level is consistent with the results of previous measurements on rare gas–halogen complexes, particularly NeBr2.

Condensation Coefficient Measurement For Water In The Umr Cloud Simulation Chamber, Donald E. Hagen, John L. Schmitt, Max B. Trueblood, John C. Carstens, Daniel R. White, Darryl J. Alofs

Physics Faculty Research & Creative Works

A systematic series of condensation coefficient measurements of water have been made using the University of Missouri-Rolla cooled-wall expansion chamber which simulates the thermodynamics of cloud. This coefficient is seen to decrease from a value near unity, at the outset of simulation, to a value in the neighborhood of 0.01 toward the end of a simulation. Final values of this coefficient are sufficiently low as to contribute significantly to the broadening of the drop-size distribution in cloud.

A Complete Set Of Elastic Constants Of Crystalline Anthracene By Brillouin Scattering, R.C. Dye, Craig J. Eckhardt

Craig J. Eckhardt Publications

All 13 elastic constants of a vapor grown, uncut, anthracene single crystal were determined from acoustic phonon velocities obtained by the Brillouin scattering method. The phonon velocities are plotted for three crystallographic planes containing the crystal axis. The relationships between phonon velocities and lattice dynamics are discussed. A minimizing procedure is introduced for converting phonon velocities of low symmetry systems into elasticity coefficients. This is shown to have several advantages over previous methods used. The results are compared with those obtained by previous studies of anthracene elastic constants.

Measured Spectra Of The Hygroscopic Fraction Of Atmospheric Aerosol Particles, Darryl J. Alofs, Donald E. Hagen, Max B. Trueblood

Mechanical and Aerospace Engineering Faculty Research & Creative Works

The relation between dry diameter (X₀) and critical supersaturation (S_c) for atmospheric submicron aerosol particles is investigated using a long term air sampling program at Rolla, Missouri. The particles are passed through an electrostatic aerosol size classifier, and then through an isothermal haze chamber. Results are reported in terms of an apparent volume fraction of soluble material, ε_v defined such that for particles composed only of ammonium sulfate and water insoluble compounds, ε_v is the actual volume fraction of soluble material. The probability distribution of ε_v is found to be approximately Gaussian in …

A Theoretical Study Of Models For X2y2 Zintl Ions, Robert J. Cave, Ernest R. Davidson, Philippe Sautet, Enric Canadell, Odile Eisenstein

All HMC Faculty Publications and Research

Ab initio and extended Hückel calculations have been used to discuss the bonding scheme in X₂Y₂ neutral and ionic main group clusters. A qualitative analysis suggests that two different electron counts, 20 and 22, are possible for the butterfly structures of these systems. This results from two orbital crossings in the correlation diagram for the tetrahedral (T_d) -> butterfly (C_2v) -> square-planar (D_2h) transformation. Detailed ab initio computations substantiate this analysis and show that the 20-electron butterfly structure becomes increasingly favored over the tetrahedral one in X₂Y₂ clusters when the 2 atoms have increasing electronegativity difference. …

Dynamics Of Triplet States In Organic Conductors By Esr Measurements, Juana Acrivos, M. Chen Lei

Faculty Publications, Chemistry

No abstract provided.

Soil-Plant-Sheep Organochlorine Pesticide Research, Tony Albertsen

Experimental Summaries - Plant Research

1. Soil O/C decay (5812)

Locations: Brookhampton, Carbunup, Dardanup, Denmark, Donnybrook, Goodwood, Jindong, Kirrup, Manjimup, Pemberton, Redmond.

To determine the rate of decay of soil Dieldrin.

2. Soil profile distribution of O/Cs (5812) (88AL21/1)

To determine the distribution of O/cs at 1 cm intervals down the soil profile.

3. Soil decontamination of O/Cs (5812)

To assess practical and economic techniques for decontaminating soil Dieldrin residues.

4. The accumulation and rundown of Dieldrin in wethers. (5841)

To determine the relationship between the levels of Dieldrin contamination in soil, pasture, sheep body and wool fat.

5. Surface drainage trial 86V1/5164

Efficient surface …

Rotational Experiments., C. K. Revell

Experimental Summaries - Plant Research

Trial 82M47

a. Subterranean clover.

Subterranean clover rotation trial
Merredin Research Station.

Trial 88ME83

b. Santiago burr medic

Rotational systems for burr medic
Merredin.

Trial 89N29

Rotational systems for burr medic.
Newdegate Research Station.

Electronic Structure Of Pb2sr2prcu3o8 As Studied By Resonant Photoemission Spectroscopy, D. S. Dessau, Z. X. Shen, P. A.P. Lindberg, B. O. Wells, Anne Borg, I. Lindau, W. E. Spicer, Joseph V. Waszczak, Lynn Schneemeyer

Department of Chemistry and Biochemistry Faculty Scholarship and Creative Works

Resonant photoemission spectroscopy has been used to study the electronic structure of the recently discovered high-temperature superconductor Pb2Sr2PrCu3O8. The valence-band features are compared with those from other high-temperature superconductors and with band-structure calculations. A strong Cu 3d8 satellite arises at approximately the same energy position as in the other high-Tc superconductors, implying that to first order the model parameter Udd will be quite similar. A 0.7-eV shift to higher binding energy of the band-theoretical density of states is required to obtain the best agreement with the photoemission spectra. The strong Cu 3d8 satellite and the discrepancy between the band theory …

Identification Of A Variant Form Of The Human Estrogen Receptor With An Amino Acid Replacement, T. Garcia, M. Sanchez, J. L. Cox, P. A. Shaw, J. B. A. Ross, S. Lehrer, B. Schachter

Chemistry and Biochemistry Faculty Publications

Estrogen receptor (ER) is a ligand-dependent transcriptional regulator. ER protein has several functionally distinct regions, including a DNA-binding domain in its midportion, a ligand binding domain in the carboxy terminal region, and at least one domain, in the amino terminal region, that influences the magnitude of transcriptional enhancement (1,2). We now report the sequence of a frequently occurring ER genetic variant that carries two point mutations in the region of the gene encoding the amino terminal domain of the protein.

The Exchange Potential In Path Integral Studies: Analytical Justification, Randall W. Hall

Collected Faculty Scholarship

We present analytical justification for our previously described exchange pseudopotential. We show how the fermi quantum partition function can be constructed from the Boltzmann (distinguishable particle) wave functions if the states that correspond to like‐spin electrons occupying the same quantum state are excluded. A class of weighting functions that satisfy this constraint approximately is discussed. Our previous pseudopotential falls under this class. Essentially, our pseudopotential forces the unwanted states to have high energy and, hence, to make negligible contribution to the partition function. Exchange potentials of the form discussed in this article should be useful for studying systems where the …

Nitrogenous Fertilizers For Cereals., M. G. Mason

Experimental Summaries - Plant Research

Trial 87CI

Rates of nitrogen on continuous wheat on heavier land.
Location: North Mullewa Research Station.

Trial 80WH3, 80MI, 80N3.

Effects of Agras No. 1 on soil pH and wheat yield.
Location: Wongan Hills Research Station, Merredin Research Station, Newdegate Research Station.

Trial 78WH3

Effect of stubble treatment on nitrogen fertilizer requirement.
Location: Wongan Hills Research Station.

Trial 89WH18

Effect of banding sources of nitrogen on grain yield.
Location: Wongan Hills Research Station.

Trial 89M2 (a)

Nitrification of sources of nitrogen on heavy soil.
Location: Merredin Research Station.

Trial 89M2 (b)

Nitrification of sources of nitrogen on heavy soil.
Location: …

Tactical Nitrogen Decisions For Wheat (And N Rates Trials), J. W. Bowden, R. J. Lunt

Experimental Summaries - Plant Research

Trial 89NO77 (89NO76)

Location: Grass Valley.

History: 1984 Lupins.

1985/86 pasture legumes and about 100 kg super/ha.

1987 Wheat yield of 2.8 t/ha using 45 kg DAP/ha and 60 of urea 2-4 WAS.

1988 Gungurru lupins (27 kg/ha seed) yielding 1.9 t/ha using 80 kg super/ha.

Tactical nitrogen trials - summary of treatments required.

Trial 89NO77

Location: Meckering

Treatments existed of nitrogen and urea. Nitrogen was not applied at seeding. Urea was applied at seeding.

Trial 88No77

Tactical nitrogen trials. - summary of observations.

Location: Grass Valley.

Trial 89NO76

Hand harvest 23/11/1989.
Yield components for copper sufficient plots reps. I …

Pasture Species Investigations., D. A. Nicholas

Experimental Summaries - Plant Research

Trial 87V7, 87MT52, 87MA31

Selection of subclover cultivars - mid to late maturing.

To select in the field subterranean clover cultivars of mid to late maturity having superior persistence and productivity.

Location: Vasse Research Station, Mount Barker Research Station, Wilgarup.

Trace Element Nutrition. Tissue Testing Programme 1989. Residual Value. Manganese Nutrition Of Lupins., R. F. Brennan

Experimental Summaries - Plant Research

Evaluation of existing diagnostic procedures for the recommendation of trace element fertilizers.

To compare the accuracy and reliability of a range of tissue and soil tests being variously promoted for use to farmers for the diagnosis and prognosis of zinc and copper deficiencies in wheat crops on Western Australian farms.

Trial 66N14

Long term copper residual trial at the Newdegate Research Station.

To determine the residual value of previously applied copper.

Location: Newdegate Research Station.

Trial 86N27

Residual value of zinc with high analysis nitrogen and phosphorus fertilizer.

To determine the decline in effectiveness of zinc on this soil types …

Soil Acidity And Eastern Wheatbelt Plant Nutrition., W. Porter., C. L. Kipling

Experimental Summaries - Plant Research

1. Affects of soil acidity on subterranean clover growth in the cropping areas of Western Australia.

Trial 87AL35, 87NO2, 87No3, 88NO76, 88NO77, 88TS54

To investigate the responsiveness to lime of subterranean clover based pastures on acid soil and the cropping areas of Western Australia.
To determine the reasons for responses to line in the field, particularly the possible involvement of legume nodulation effects and the alleviation of soil/water repellence.
To develop practical identification and management strategies for acid soils problems.

Trial 87AL35.

Liming in crop/pasture rotation on acid soils

Location: Woogenellup.

History: 1987 Lime applied, sown to barley
1988 Clover …

The Nitrogen Response Of Wheat Crops Following Lupins, I C. Rowland, Mel Mason, John Hamblin

Journal of the Department of Agriculture, Western Australia, Series 4

One of the many benefitsof growing narrow-leaved lupins in a crop rotation (Rowland et al 1988) in the increased yield of the following cereal crop as a result of the improved nitrogen level of the soil.

This article reports the results from 41 wheat/lupin rotation trials involving a range of nitrogen rates throughout the Western Australian agricultural area between 1978 and 1987. These trials allowed scientists to assess the interaction of nitrogen fertilization with previous cropping history and to estimate some of the contribution of the nitrogen and other effects of lupins within the crop rotations. The results provide some …

Choosing A Calcium Supplement For Sheep Fed Cereal Grains, R L. Peet, Michael Hare

Journal of the Department of Agriculture, Western Australia, Series 4

Sheep fed cereal grains as an energy source over summer usually need added calcium because cereal grains are generally low in calcium (for example, 0.03 per cent calcium compared to 0.26 per cent phosphorus), and there may not be a natural source such as clover, weeds, leafy stubble or edible bush in the paddock.

The safestt and most effective calcium supplement is finely ground limestone added to the feed, but some farmers have used other sources of calcium such as gypsum and superphosphate in this manner.

Intensive Animal Industries In The Peel-Harvey Catchment, R W. Payne

Journal of the Department of Agriculture, Western Australia, Series 4

The Swan coastal plain to the north and south of Perth is attractive for the intensive production of pigs and poultry for several reasons. It is close to markets, feed manufacturers and labour; has a less extreme climate than inland areas; and is underlain by a large reserve of high quality groundwater.

Because these are all intensive operations, large quantaties of nutrient-rich waste are produced over a very small area. They can severely damage ground and surface waters unless steps are taken to control the wastes which they produce.

This article oulines both the nature of intensive aniamal industries in …