Digital Commons Network^™

Isolation, Purification And Partial Characterization Of Some Isoenzymes Of Human Serum Alkaline Phosphatase, M. Sue Jones

Morehead State Theses and Dissertations

A thesis presented to the faculty of the School of Sciences and Mathematics Morehead State University in partial fulfillment of the requirements for the Degree of Master of Science by M. Sue Jones on July 18, 1977.

A Floristic Analysis Of The Sevilleta Wildlife Refuge And The Ladron Mountains, G. Thomas Manthey

Biology ETDs

The Sevilleta Wildlife Refuge and Ladron Mountains of westcentral New Mexico were selected for a floristic inventory and analysis because of a lack of previous study in this unique area. A key and annotated checklist of the vascular flora of the study area were compiled. The 1140 taxa representing this floristically diverse area were documented by either (i) specimens in the University of New Mexico Herbarium or (ii) specimens collected during 1975 and 1976. Twelve floristic communities are recognized. These are based upon descriptive field notes, species lists from collection sites, and photographs taken during collection trips. The communities are …

Leaf Tissue Production In Relation To Succession In The Spruce-Fir Forests Of Northern New Mexico, Craig F. Eberhart

Biology ETDs

Leaf tissue production was studied to determine possible successional trends. The study area was in the spruce-fir zone of the southern Rocky Mountains 15 kilometers northeast of Santa Fe, New Mexico. In this area quaking aspen (Populus tremuloides) is a successional species that is slowly replaced by Engelmann spruce (Picea engelmannii) and corkbark fir (Abies lasiocarpa var. arizonica) as succession proceeds towards the classical spruce-fir climax. Five study plots were subjectively selected to represent a successional sequence of forest stands with similar physical characteristics. The plots were arranged in a successional series by using the increase in coniferous basal area …

An Evaluation Of Reproductive Isolation And The Possibility Of Hybridization Between The Rattlesnakes Crotalus Atrox And Crotalus Scutulatus, James S. Jacob

Biology ETDs

The possibility that Crotalus atrox and C. scutulatus might hybridize in southeastern Arizona-southwestern New Mexico or in the Big Bend region of Texas was analyzed morphometrically and electrophoretically. Interbreeding of captive Crotalus, and intraspecific variation and interspecific overlap in key morphological characters has led to the incorrect assumption that these species hybridize. Even though they are syntopic primarily in an unstable desert-grassland ecotone where habitat differences are not evident, C. atrox and C. scutulatus are morphologically and biochemically distinct. The apparent lack of geographic, mechanical, and ecological premating isolating mechanisms in syntopic populations off C. atrox and C. scutulatus suggests …

Effects Of Water Stress And Temperature-Photoperiod Preconditioning On Responses Of Atriplex Canescens (Pursh) Nutt. Seedlings To Drought, Barbara Gail Hjelle

Biology ETDs

Fourwing saltbush [Atriplex canescens (Pursh) Nutt.] seedlings were subjected to water stress treatments and temperature-photoperiod regulation to determine if these treatments might induce greater hardiness to subsequent drought stress. Seedlings ages 2, 4, and 8 weeks after emergence were allowed to wilt before rewatering or kept at minimum soil moisture stress. Seedlings aged 2 weeks after emergence were subjected to four regimes of hot and cold temperatures and long and short photoperiods in growth chambers. Treatments were applied for four weeks. Height, fresh weight and dry weight of shoots, and dry weight of roots were reduced by water stress and …

Chromosome Counts Of Compositae From The United States And Mexico, Donald J. Pinkava, David J. Keil

Biological Sciences

Chromosome counts are reported for 126 taxa representing 122 species and 61 genera of Compositae. First reports include two genera, Stylocline (n = 14) and Chromolepis (n = 19), 17 species, two infraspecific taxa, and one interspecific hybrid. Five additional taxa have chromosome numbers differing from previously published accounts. Carminatia is reinstated to generic status.

The Effects Of Multiple Fin Amputations On Rates Of Fin Regeneration In Goldfish, Carassius Auratus (Linnaeus), Thomas Kozicki

Masters Theses & Specialist Projects

Goldfish, Carassius auratus (Linnaeus), were used in the study of rates of regeneration in all possible combinations of caudal, dorsal, left pectoral, and right pectoral fin amputations. The purpose of this study was to ascertain the effects of multiple injuries on rates of regeneration, to determine rates of regeneration in different fins, to test the hypothesis that rates of regeneration decrease from the proximal to the distal areas of a fin, and to determine the presence or absence of morphogenetic gradients in fin regeneration.

In almost all cases, no significant differences in total regeneration were found to occur when the …

Chromosome Counts Of Compositae From Mexico And The United States, David J. Keil

Biological Sciences

Chromosome counts of Compositae are reported from Mexico and the United States. First generic counts are from Egletes (n = 27), Hydropectis (n = 9), and Pippenalia (2n = ca. 60). First counts also are reported for 22 specific and infraspecific taxa in Baccharis, Erigeron, Flaveria, GnaphaUum, Grindelia, Helenium, Heterotheca, Melampodium, Montanoa, Perymenium, Piqueria, Senecio, Stevia, Verbesina, Xanthocephalum, and Zaluzania. Additional counts also are provided for 123 populations of taxa counted previouslY, of which eight are new numbers. Taxonomic implications of certain counts are discussed.

A Study Of Photoperiodic Time Measurement Of Samples From A Michigan Population Of Orconectes Immuni (Hagen), E. Lynn Talton

Masters Theses & Specialist Projects

Experiments were conducted to determine if an hourglass model is the mechanism whereby photoperiodic time is measured by the crayfish, Orconectes immunis. Two experiments were conducted in which there were three series of treatments. Series I and II were T experiments in which T was the total length of the light-dark cycle. In Series I the light phase of the cycle was held at 16 hours with varied lengths of darkness (LD 16:2, T 18; LD 16:8, T 24; LD 16:20, T 36; LD 16:32, T 48). In Series II the dark phase was 8 hours in length and …

Meeting Of The Elephant Interest Group At Michigan State University, June 21, 1977, Jeheskel Shoshani

Elephant

Digest of the minutes of the first informal meeting of the Elephant Interest Group at the 57th Annual Meeting of the American Society of Mamalogists (ASM).

Buoyancy Changes As Related To Respiratory Behavior In An Amphibious Snail, Pomacea Urceus (Müller), From Venezuela, Kathleen A. Burky, Albert J. Burky

Biology Faculty Publications

The ampullariid Pomacea urceus uses its ctenidium and lung in respiration. The snails reach the surface with their siphon and ventilate their lung by withdrawing their head-foot in a pumping action. The lung gas also serves to change the buoyancy of the snails. After a ventilation a snail does not necessarily return to the same buoyancy level. They may achieve overall specific gravities of <1 to > 1 after a ventilation. They may remain submerged (s.g. > 1) or float (s.g.

The Effect Of Septal Lesions And Postoperative Time Delay On A Shuttle Sidman Avoidance Task, Martin David Tier

Morehead State Theses and Dissertations

A thesis presented to the faculty of the School of Education at Morehead State University in partial fulfillment of the requirements for the Degree of Master of Arts by Martin David Tier on June 9, 1977.

The Effect Of Cv-1 Cell Geometry On The Ultraviolet Radiation Sensitivity Of Mammalian Cells In Vitro, Daniel J. Knauer

Masters Theses & Specialist Projects

The effect of cell geometry on the radiosensitivity to ultraviolet (UV) light has been studied in the CV-1 host cell-capacity system. In this system the macromolecular damage incurred by monolayers of cells cultures as the result of exposure to UV radiation is monitored by testing the ability of irradiated cells to support the replication of Herpes simplex virus. The target molecule has been shown to be DNA and is centrally located

Mammalian cells have an absolute requirement for Mg⁺⁺ and Ca⁺⁺ in order to remain attached to artificial substrata. To test the effect of cell geometry on UV …

The Effect Of Carbon Dioxide Concentration On Calcification In The Red Coralline Alga Bossiella Orbigniana, A. Dwight Smith

Loma Linda University Electronic Theses, Dissertations & Projects

The relationship between various experimental concentrations of CO2 and calcification was studied by measuring Ca-45 incorporation into the crystalline matrix. Air containing CO2 at partial pressures (Pc02) of 0.04% to 5.5% was bubbled through synthetic sea water in incubation vessels. The resultant pH values ranged from 8.7 to 6.5. The relative concentrations ofCO2, HCO3 , and CO2— 3 were calculated from the measured ' pH—PCO2 combinations. Calcification was correlated positively with HCO expressed as percent of total carbon. Maximum calcification of about one—third above normal occurred between 0.1% and 1.0% P CCOO22 At 0.26% P calcification increased as increments of …

An Analysis Of Species Recognition In Sympatric, Allopatric And Reciprocally Cross-Fostered Peromyscus Californicus And Peromyscus Eremicus (Rodentia, Cricetidae), Ronald L. Carter

Loma Linda University Electronic Theses, Dissertations & Projects

Experiments were performed to compare homospecific and heterospecific mate selection in two closely related species, Peromyscus californicus and Peromyscus eremicus. Comparisons were made between allopatric and sympatric populations and between males and females for mate selection performance. Both species made significant choice for the homospecific chambers. Significant homospecific choice was made by mice from sympatric populations, whereas allopatric populations did not demonstrate significant choice. No significant difference in choice performance was demonstrated between males and females even when the estrus stages of the females were controlled.

A comparison of different testing lengths and temporal regimes of data collection was performed …

Optimal Foraging: A Selective Review Of Theory And Tests, G.H. Pyke, H.R. Pulliam, Eric Charnov

Biology Faculty & Staff Publications

Beginning with Emlen (1966) and MacArthur and Pianka (1966) and extending through the last ten years, several authors have sought to predict the foraging behavior of animals by menas of mathematical models. These models are very similar, in that they all assume that the fitness of a foraging animal is a function of the efficiency of foraging measured in terms of some "currency" (Schoener, 1971) - usually energy - and that natural selection has resulted in animals that forage so as to maximize this fitness. As a result of these similarities, the models have become known as "optimal foraging models"; …

Limnological Aspects Of Lake Mead, Nevada-Arizona, John R. Baker, James E. Deacon, Thomas A. Burke, Samuel S. Egdorf, Larry J. Paulson, Richard W. Tew, Bureau Of Reclamation

Publications (WR)

Lake Mead is a deep, subtropical, moderately productive, desert impoundment with a negative heterograde oxygen profile occurring during; the summer stratification. investigations of the Boulder Basin of Lake Mead by the University of Nevada were initiated in November 1971. The primary objective of the study was to determine what effects industrial and sewage effluent from the Las Vegas metropolitan area, discharged into Las Vegas Bay, have had on the water quality and limnological conditions of Boulder Basin. Data from the 1975-76 period are presented in detail, with earlier data included in the summaries and discussions. Measurements of water temperature, dissolved …

Effects Of Types 1 And 2 Herpes Simplex Viruses On Several Fish Tissue Culture Systems, Raymond Charles Harry

Dissertations and Theses

The purpose of this investigation was to observe changes induced by types 1 and 2 herpes simplex viruses (HSV 1 and HSV 2) when these viruses were cultivated in fish cells. The possibility of attempting to use human strains of herpesviruses in order to transform normal fish tissue culture preparations was considered. Three different fish cell cultures were tested: CHSE-214 cell lines were derived from embryonic chinook salmon (Oncorhynchus tshawytscha), and STE-137 cell lines were obtained from embryonic steelhead trout (Salmo gairdneri). Cells isolated from immature ovaries of rainbow trout (Salmo gairdneri) were propagated …

A Behavioral Study Of A Small Group Of Pikas (Ochotona Princeps) On Mt. Hood, Oregon, Nancy A. Ewen Frazier

Dissertations and Theses

Observations or a small group of pikas, (Ochotona princeps) occupying a southwest facing roadfill on Mt. Hood, Oregon (1518 m) were made during the fall or 1976. Territorial sizes of 305.5 m² and 121 m² were determined based on territorial displays. These territories were compared with those observed in other studies. The mean distance to the nearest neighbor was 15.1 m. A pair relationship between two of the pikas and a dominance order are discussed. The seasonal progression of the haypiles and the reactions of the pikas to other animals are also noted.

Behavioral Temperature Regulation In The Turtle Pseudemys Scripta Elegans, Michael H. Johnston

Dissertations and Theses

A group of red-eared turtles (Pseudemys scripta elegans) was acclimated to 18-20˚C and artificial photo-period for 21 days. The animals were then individually placed in a thermal gradient and their selected temperatures were continuously recorded for 24 hours. A second group of turtles was acclimatized to 7.5-11.0˚C and natural photoperiods for 28 days, and subsequently tested in the same manner as the first group. Lastly, the turtles in the first group, after being acclimated to 3.0˚C and the previous artificial photoperiod for 14 days, were retested in the gradient.

Relation Of Flotation Analysis To Archeological Excavations, Chaco Canyon, New Mexico, Mary B. Struever

Biology ETDs

Flotation analysis (the examination of small-scale botanical remains recovered by a simple water separation technique) was undertaken at site 29SJ627, a small pueblo in Chaco Canyon, New Mexico. Altogether 69 samples were examined, yielding 5208 seeds from approximately 30 plant taxa. All plant taxa encountered in flotation samples are growing in Chaco today, with the exception of the domesticates corn and squash, and the riparian Scirpus (bulrush). The assemblage of seed types recovered by flotation indicates that the prehistoric plant community was essentially similar to today's, with increased emphasis on arboreal and riparian species and that weedy types were more …

Floristic Affinities Of Animas Mountain, Southwestern New Mexico, Warren L. Wagner

Biology ETDs

Animas Mountain of southwestern New Mexico was selected for a floristic inventory and analysis because of the lack of previous study in this unique portion of the Southwest.

An annotated checklist of the vascular flora of the study area was compiled. A species-rich flora represented by 718 taxa (ca. 20% of the known flora of New Mexico) was documented by either (i) herbarium specimens from the University of New Mexico, or New Mexico State University herbaria, or (ii) specimens collected during the 1975 and 1976 growing seasons.

Floristic assemblages were recognized based upon descriptive field notes, species lists from collection …

A Multivariate Study Of The Family Molossidae (Mammalia, Chiroptera): Morphology, Ecology, Evolution, Patricia Waring Freeman

Biology ETDs

The Molossidae is a worldwide, mostly tropical family of bats. This analysis examines what phenetically similar groups occur within the family; what ecological role species of molossids may have; and, finally, what kind of evolutionary relationships exist among phenetically similar species.

Seventy-six characters were taken on each of eighty species of molossids and analyzed by principal components, distance (size-in and size-out), and correlation analyses from the NT-SYS package of multivariate statistical programs. Phenetically similar bats are grouped and described morphologically. Morphological trends which are revealed by the principal components analysis are used to make ecological predictions about the bats' feeding …

Laboratory Diagnosis Of Genital Infections Associated With Chlamydia Trachomatis: Cell Culture And Serological Methods, Linda L. Cole

Biology ETDs

Recent investigations have revealed the widespread involvement of Chlamydia trachomatis in nongonococcal genital infections in man. In an effort to develop the necessary methodology for isolating this agent, several different cell culture techniques were evaluated.

Additionally, a fluorescent antibody technique and neutralization test were developed for possible diagnostic use.

Quantitation of Chlamydia in cell culture was accomplished using inclusion counts which showed linear dose response curves. Inclusions were stained by the PAS, iodine, and Giemsa methods, and these stains were found to be equal in their sensitivity in detecting chlamydia inclusions. Using inclusion counts as a quantitative assay, a variety …

Relation Of Pollen Analysis To Archeological Excavations, Chaco Canyon, Anne C. Cully

Biology ETDs

This study was begun in the summer of 1975 at site 29SJ627, In Marcia's Rincon, Chaco Canyon, New Mexico. Dates of the occupation of the site were from ca. 800 A.D. to 1050 A.D. The primary purposes of the study were to identify pollen; investigate factors affecting variability, including temporal, functional, and spatial factors; investigate sampling techniques to obtain adequate representation of variability; comparison of pollen and flotation samples. A total of 83 samples were processed and 34 taxa were identified. Within site 29SJ627 a block of rooms was chosen for extensive sampling. Within the rooms a grid system was …

A Preliminary Investigation Of The Lipid Of Halophilic Bacteria As A Food Additive, Neil F. Collins

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

This study was conducted to evaluate the feasibility of obtaining workable quantities of the unusual ether-linked phosphatidyl glycerophosphate of halophilic bacteria through batch culture of Halobacterium halobium. Once isolated and purified, physical, emulsification, and toxicological properties of the lipid were evaluated with respect to its use as a nonnutritive lipid substitute or an emulsifier by the food industry. These evaluations included physical descriptions, melting properties, hydrophile-lipophile balance (HLB) determination, and oral and intraperitoneal administration of the lipid to laboratory mice.

Fine Structural Aspects Of The Developing Eye Of The Honey Bee, Judith Susan Eisen

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The post-embryonic development of the compound eye of the worker honey bee was investigated with the light and electron microscopes. The cells of the optic primordium were reorganized into preommatidial cell clusters during the late third and early fourth larval instars. Each cluster contained nine prospective retinula cells, the ninth was centrally located and shorter than the other eight; four prospective cone cells; and an undetennined number of prospective pigment cells.

Rhabdomere development began in the eight peripherally located retinula cells just prior to pupation. Following pupation, each ommatidium elongated. During ommatidial elongation, the short ninth retinula cell moved from …

Community Structure Of Ten Southwestern Rodent Faunas, Terrence Morgan May

Biology ETDs

Thirty morphological characters were measured for each of 63 rodent species. All characters were standardized to a mean of zero (O) and standard deviation equal to one (1). From this assemblage, subsets were chosen to represent ten (10) naturally occurring rodent communities of New Mexico and Arizona. Numerical taxonomy techniques of distance, correlation and principal components analysis were used to analyze the data.

Results reveal three distinct community 'types'. The presence or absence of morphologically divergent species is responsible for these differences. The appearance of Castor canadensis (the most morpho-logically divergent species) is dependent upon the addition of a new …

The Distribution Of Orcuttia Californica (Poaceae) In The Vernal Pools Of The Santa Rosa Plateau, Riverside County, California, Cluney M. Stagg

Loma Linda University Electronic Theses, Dissertations & Projects

Ecological studies were conducted on the rare annual grass Orcuttia californica (Vasey), an endemic to California, from April to September, 1976 in 11 vernal pools located on the Santa Rosa Plateau in Riverside County. To help determine why Orcutt grass occurs in only 6 of the 11 pools and more specifically why it only occurs in patches within a given pool, biweekly measurements of frequency, density and phenology were taken within each of a total of 28 circular plots (r = 10 meters) in 5 pools. This data was related to concurrent biweekly measurements of soil moisture, soil texture and …

Density And Diversity Responses Of Summer Bird Populations To The Structure Of Aspen And Spruce-Fir Communities On The Wasatch Plateau, Utah, Janet Lee Young

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Sixteen stands representing a range of structural variation in aspen, mixed aspen-conifer, and spruce-fir communities of the Wasatch Plateau, Utah, were censused by the sample count method. The stands were classified as eleven community types based on the understory dominants or indicator species and the cover types. Fifty bird species were recorded during the two seasons; thirty-two occurred in aspen cover, forty-four in mixed aspen-conifer cover, and twenty-two in spruce-fir.

Comparisons of the composition and density of bird populations were made between uniform stands of a single life form and more structurally complex stands of either single or mixed life …