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Sexual Differentiation Of The Rat Hypothalamus: An Evaluation Of Sexual Behavior And Hypothalamic Morphology Following Neonatal Manipulation, Michael C. Stamper

Morehead State Theses and Dissertations

A thesis presented to the faculty of the School of Sciences and Mathematics at Morehead State University in partial fulfillment of the requirements for the Degree of Master of Science in Biology by Michael C. Stamper on August 24, 1984.

Effects Of Dehydration On Hemoglobin Oxygen Affinity And Blood Cell Volume In Two Anurans, Andrew Christopher Zygmunt

Dissertations and Theses

Two aspects of possible adaptation in cardiovascular performance caused by increased plasma electrolytes were examined. Cells in anisotonic plasma may either act as osmometers or volume regulate. Blood flow rate is dependent upon cell viscosity, which in turn is a consequence of cell volume and membrane deform-ability. Cell volume changes which increase membrane deform-ability will thus potentially extend the limits of dehydration tolerance. It was found in R. catesbeiana and B. marinus that red blood cell is maintain constant volume during dehydration. Cells in vitro initially lose water, but then sodium, potassium and water move into the cell. Cell viscosity …

In Vitro Glucose Utilization In Ob/Ob Mouse Erythocytes & Adipose Tissue, Peggy Fudge

Masters Theses & Specialist Projects

An in vitro study of ob/ob mouse and control mouse erythrocytes and adipose tissue showed a difference in the rate of glucose utilization as determined by the production of ³H₂0. In both ob/ob and control erythrocytes, glucose utilization increased as glucose concentration of the medium was increased, although the ob/ob rate was consistently lower than the control. The similar trend except for the adipose tissues showed a ob/ob adipose tissue at 200 mg/d1 glucose concentration which exhibited a rate less than the rate at 100 mg/d1.

The decreased glucose utilization by adipose tissue in the absence of …

Studies On The Binding Of Human Chorionic Gonadotropin To Polystyrene & A Possible Application, Patrick Mowers

Masters Theses & Specialist Projects

Human chorionic gonadotropin (hCG), labeled with 125_I, was used to study the binding properties of hCG to polystyrene beads. The binding capacity of the 0.25-in diameter beads was determined to be approximately 300 mIU/bead. The affinity constant, K, was determined to be essentially O, suggesting that binding is strictly passive. The binding of hCG to polystyrene was proportional to the hCG concentration and temperature. There were highly significant (p<0.001) differences among amounts of hCG bound at 3 C, 25 C and 37 C. There was a highly significant (p<0.001) relationship between the amount of hCG bound and the initial hCG concentration. There did not appear to be any correlation between the percentage of hCG bound and the initial hCG concentration. Attempts to use Staphylococcal Protein A-peroxidase conjugate to detect murine monoclonal antibodies to beta-hCG were not successful.

An Evaluation Of Naturally Occurring Populations Of Magnolia Tripetala L. In Indiana, Kemuel S. Badger

All-Inclusive List of Electronic Theses and Dissertations

Magnolia tripetala L. occurs in Indiana as a disjunct from the Appalachian mountains populations. Currently, the known distribution of ~· tripetala in Indiana is confined to seven sites in Crawford County. The current status of ~· tripetala and the surrounding plant community was evaluated to aid in the future study, protection and management of this species, which is endangered in Indiana. Systematic random sampling of the tree stratum was done with belt transects. The sapling and seeding strata were sampled with subplots, placed along the belt transects. Permanent plots were established to enable future monitoring of what is currently prime …

An Evaluation And Comparison Of Various Diversity Indices As Illustrated By Portage Creek, Karl Josef Siebert

Masters Theses

The diversity index is an established method of measuring water quality. In this study various diversity indices are evaluated for their use in a rapid, accurate, and cost effective biological monitoring program. The study investigated five diversity indices: Approximate, Brillouin's, Shannon's, Simpson's and the Sequential Comparison Index. The diversity indices were tested for accuracy and sensitivity on actual on actual macroinvertebrate samples and simulated data.

The Simposon's Index was found to provide the most accuracy and sensitivity for the indices tested. The importance of considering the evenness element of diversity indices was also supported by the study.

Chemical Leaf Repellency To An Attine Ant: Seasonal Distribution Among Potential Host Plant Species, Jerome J. Howard

Biological Sciences Faculty Publications

The chemical repellency of leaves to the leaf-cutting, fungus-growing ant Atta cephalotes was surveyed for 42 plant species randomly selected from the dry forest of Santa Rosa National Park, Guanacaste, Costa Rica. The sample represents about one-sixth of the potential host plant species available to the ants in the secondary, semideciduous forest at Santa Rosa. Repellencies of leaf extracts were measured by bioassay. A laboratory colony of A. cephalotes collected from Santa Rosa was used. Three-quarters of the species exhibited significantly repellent nonpolar (lipid-soluble) extractables (e.g., terpenoids, steroids, and waxes), and half of the species exhibited repellent polar extractables (e.g., …

Studies On The Phytoplankton Of Littoral Waters Of The Arabian Gulf As Impacted By Oil Spills, Hamdan S. Al-Ghamdi

Masters Theses

A study of the effect of oil pollution on two intertidal areas of the Saudi Arabian Gulf coast was undertaken. One area was severely impacted by oil; the other was relatively clean. The organisms studied also indicated the effects of pollution were diatoms and blue-green algae.

Members of the diatom genus Amphora were more numerous in the clean water area than in the polluted section, confirming their preference for relatively clean habitats. Members of the diatom genus Asterionella, a more resistant group, were much more abundant in the polluted area.

Blue-green algae which also tolerate pollution were also more abundant …

The Role Of Colonization Predation And Season In Determining Macroinvertebrate Community Structure In A Temperate Lake, Cheryl Lynn Frew

Biological Sciences Theses & Dissertations

Field experiments were conducted to examine the effects of distance from shore, vertebrate predation, and season on macroinvertebrate colonization dynamics in a temperate lake in Suffolk, VA. In a year-long colonization study, artificial plants, half of which were caged to exclude vertebrate predators, were deployed in patches at three distances from shore (2m, 15m, 50m). Artificial plant subsamples were removed at weekly intervals over 28 days during four seasons to monitor colonization by macroinvertebrates. Colonization of artificial plants occurred more quickly in the summer and spring than in fall and winter. The interactive effect of distance from shore and cage …

Effects Of Intraperitoneal And Intracerebral Injections Of Triiodothyronine On Temperature Selection In Fish, Dennis A. Grahn

Dissertations and Theses

Goldfish (Carassius auratus) of 15-28g were maintained at 23 C and subsequently were intraperitoneally injected with saline (controls) and varying doses of Triiodothyronine (T₃). Following the injection, the fish were placed in a thermal gradient and temperature selection and activity monitored for 30 min. There was no- significant difference between the responses of the experimental fish to the 2 different doses of T₃. However, there was a significant difference in response between the experimental and control groups (P < 0.001). The decreased temperature selection occurred early in the test runs, while the increased activity began later. Larger goldfish (175-205g) received 0.5 μl intracerebral injections of 1.5-3.0 ng T₃ in the anterior brainstem via an indwelling cannula injection system. These fish responded to the injections by …

A Bacteriological And Chemical Analysis Of Nonpoint Source Pollution In A Karst Aquifer Bowling Green, Kentucky, Wayne Green

Masters Theses & Specialist Projects

Monthly water samples collected from four sites in the Lost River Groundwater Basin, a shallow karst aquifer in the Bowling Green-Warren County area of Kentucky, represented samples from sites receiving conduit and diffuse flow. All sites were severely contaminated with bacteria, and on some occasions the surface water criteria for some heavy metals were exceeded.

Of the total 334 bacterial colonies identified 92.1% were verified as Escherichia coli by the API20E system. Acinetobacter calcoaceticus var. anitratum accounted for 2.10% of colonies; Citrobacter freundii for 0.30% Klebsiella pneumoniae for 0.90%; Klebsiella oxytoca, 0.90%; Citrobacter amalonaticus 0.30%; Enterobacter cloacae, 1.20%; …

Phenotypic Differences In Populations Of Euonymus Americanus L. From The Central United States & Autecological Studies Of This Species In A Deciduous Forest Of Kentucky, Paul Bayer

Masters Theses & Specialist Projects

The concentrations of sodium and potassium in the leaves of Euonymus americanus collected from a relict hardwood forest during a four month period showed a possible winter conditioning pattern. Seeds collected from this same relict stand would not germinate, even though they were subjected to a wide variety of germination experiments. Phenotypic differentiation in fully developed leaves was not found; however, differences related to the length of the growing season were noted. Autecological studies of Euonymus americanus should be continued to investigate the role of this plant in relict hardwood stands.

Ua66/6/2 Alumni Newsletter, Wku Biology

WKU Administration Documents

Newsletter created by and about the WKU Biology department highlighting students, faculty and alumni activities.

Effects Of Adrenalectomy On Hemoconcentration And Blood Ph, Urea And Electrolytes In The Mongolian Gerbil (Meriones Unguiculatus), Magally Aellos De Cova

Master's Theses

The effects of adrenalectomy on some blood profiles of the Mongolian gerbil, Meriones unguiculatus, were studied in order to clarify the possible causes of its short survival after adrenal ablation. Measurements of hematocrit, pH, urea and electrolytes were made 18, 24 and 30 hours after surgery. Results show the expected alterations: hemoconcentration, metabolic acidosis, high values of urea and disturbances in electrolytes. While hyponatremia was significant only after 24 hours, important hypochloremia was not present at any time. Hyperkalemia was the most significant alteration observed. It occurred as early as 18 hours after surgery, suggesting that it may be an …

Source Of Inoculum, Yield And Quality Of Tomato As Affected By Leveillula Taurica, Wayne B. Jones

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Tomato transplant stock from southern Nevada served as a source of inoculum and developed disease symptoms of tomato powdery mildew approximately three weeks before Utah-grown transplants. Plants from Nevada and Utah were equally infected seven weeks after planting and no differences in severity or yield were detected at the end of the season due to rapid spread of the fungus. Tomato yields were reduced ca 40% in check plots when compared to plots treated with triadimefon, propiconazol or sulfur. The amount of sunburned fruit in check plots was three to four times greater than in plots where the disease was …

Seasonal And Spatial Heterogeneity In The Limnetic Zooplankton Community Of Lake Mead, Gene Robert Wilde

Publications (WR)

Zooplankton samples collected from throughout Lake Mead, in 1981-1982, demonstrate the presence of a statistically significant seasonal and spatial heterogeneity in zooplankton densities. Seasonally, the major zooplankton groups were most abundant in the spring and fall, coincident with maxima in chlorophyll-a concentrations. Successions among the various rotifers, cladocerans and copepods present in the reservoir were influenced by food availability, diapause, predation by planktivorous fish and, possibly, water temperatures. Spatial heterogeneity in zooplankton densities was unrelated to water temperature, pH, conductivity and dissolved oxygen concentrations, but was related to the abundance of phytoplankton (chlorophyll-a concentrations) and fish. Statistical analyses indicate that …

The Role Of Nannoplankton In The Phytoplankton Dynamics Of Four Colorado River Reservoirs (Lakes Powell, Mead, Mohave, And Havasu), Jeffrey John Janik

Publications (WR)

Phytoplankton species composition and community size structure were studied in four warm-monomictic Colorado River reservoirs; lakes Powell, Mead, Mohave, and Havasu from March 1981 to February 1982. Sampling was done at approximately monthly intervals from several stations in each reservoir. The Utermohl technique was used to enumerate phytoplankton. The phytoplankton assemblage was divided into the following six size classes using microscopic techniques; netplankton (>64 um), and nannoplankton (>5, 5-11, 12-21, 22-44, and 45-64 um).

Total phytoplankton biomass and community size structure were different among these four reservoirs with considerable spatial and temporal variation present. Average reservoir-wide areal weighted …

Vocal Behavior And Interactions Among Parents And Offspring In The American Dipper, Mary Kathleen Fite

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The American Dipper (Cinclus mexicanus), an inhabitant of rapidly flowing mountain streams, is an aquatic passerine whose vocalizations have previously been undescribed. This study examines the spectrographic characteristics and contexts of calls and song in a color-banded population of Dippers. Calls of adults are the Jih, Weep and Grawk calls; calls of nestlings and fledglings are the Chip and Beg calls. Adult Dippers have a limited number of call types which occupy a middle range of frequencies (3 to 6kHz) and have structural characteristics which enhance detection of vocal signals. Gradation is present between call types. Possible explanations …

Effects Of Folic Acid Or Zinc Malnutrition On Rotaviral Infection In A Murine Model, John Douglas Morrey

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The influences of dietary deficiencies of folic acid or zinc on rotaviral disease infant mice were studied. Preliminary studies indicated that bovine and simian rotaviruses, but not porcine rotavirus, caused diarrhea in infant mice. Bovine and porcine rotaviruses were not, however, sufficiently infectious to replace murine rotavirus in studies utilizing the murine model. It was also determined that murine rotavirus purified by a cesium chloride gradient was highly infectious and useful for subsequent studies on nutritional influences of rotaviral disease. In dietary studies, female Swiss Webster mice were fed diets containing deficient, moderately deficient, or adequate levels of folic acid …

Isolation Of A Collagenase Cdna Clone And Measurement Of Changing Collagenase Mrna Levels During Induction In Rabbit Synovial Fibroblasts., Robert H. Gross, Lynn A. Sheldon, Colin F. Fletcher, Constance E. Brinckerhoff

Dartmouth Scholarship

To facilitate our studies on the mechanisms controlling collagenase production at a molecular level in rabbit synovial fibroblasts, we have constructed a cDNA library using mRNAs isolated from cells induced with crystals of monosodium urate monohydrate. We have screened this library with cDNA probes made from induced and control mRNA populations. From among 30 clones that hybridized preferentially to the induced-cell probe, 4 contained collagenase sequences. The largest, a clone of 650 base pairs, was identified by its ability to hybrid select a mRNA that could be translated in a cell-free system into a product that was precipitable with monospecific …

Book Review Forum On Derek Freeman's Margaret Mead And Samoa: The Making And Unmaking Of An Anthropological Myth, Fay Ala‘Ilima, Tuaopepe Felix S. Wendt, Nancy Mcdowell

Pacific Studies

No abstract provided.

Rhizobium Phaseoli Symbiotic Mutants With Transposon Tn5 Insertions, K. Dale Noel, Araceli Sanchez, Leonor Fernandez, Jan Leemans, Miguel A. Cevallos

Biological Sciences Faculty Research and Publications

Rhizobium phaseoli CFN42 DNA was mutated by random insertion of Tn5 from suicide plasmid pJB4JI to obtain independently arising strains that were defective in symbiosis with Phaseolus vulgaris but grew normally outside the plant. When these mutants were incubated with the plant, one did not initiate visible nodule tissue (Nod-), seven led to slow nodule development (Ndv), and two led to superficially normal early nodule development but lacked symbiotic nitrogenase activity (Sna-). The Nod- mutant lacked the large transmissible indigenous plasmid pCFN42d that has homology to Klebsiella pneumoniae nitrogenase (nif) genes. The other mutants had normal plasmid content. In …

A Survey Of Larval Caddisflies (Insecta: Trichoptera) From Riffles Of Three Streams Of The Drake's Creek Drainage, Kentucky, With A Preliminary Investigation Of The Adult Trichoptera Of The Region, Michael Beiser

Masters Theses & Specialist Projects

A total of 1665 larval caddisflies, representing four families and eight genera, was collected from seven stations in the Drake's Creek drainage from 1 November, 1982 through 15 October, 1983. The pattern of longitudinal distribution of the larvae, based on feeding relationships, in the West and Middle Forks of the system was consistent with studies by Wiggins and Mackay (1978), Andrews and Minshall (1979), and Ross and Wallace (1982) and the River Continuum Concept (Vannote et al. 1980). The Trammel Fork, a spring fed stream, showed a pattern of distribution contrary to the predictions of the Concept, suggesting that the …

Activation Of 2-Aminoanthracene By Cell-Free Microbial Extracts, Thomas Hamill

Masters Theses & Specialist Projects

The activation of the precarcinogen 2-aminoanthracene was investigated employing cell-free extracts of Escherichia coil, Pseudomonas aeruginosa, Streptococcus faecalis, Bacteroides fragilis, Clostridium perfringens and the eukaryote Candida albicans. A modification of the Salmonella typhimurium/mammalian microsomal mutagenicity assay was employed to determine activation. Of the species tested, only cell-free extracts of Escherichia coli and Bacteroides fragilis failed to induce the reversion of the Salmonella typhimurium his- mutants TA-98 and/or TA-100 to his+ revertants.

Separation of the cytosol and membrane pellet enzymes was completed. Of the organisms tested only the membrane pellet enzymes of Clostridium perfringens showed activation of …

The Development Of Anatomical Features In Larval Fish In Conjunction With The Onset Of Exogenous Feeding, Irene Kokkala

Masters Theses & Specialist Projects

Observations were made on the appearance and development of anatomical features such as neural receptors (free neuromasts and taste buds), lateral line canals and bone elements of the mandibular and hyoid regions of the skull associated with the larval stages of the spotted bass (Micropterus punctulatus) and northern hog sucker (Hypentelium nigricans). The results were related to known feeding habits and habitat selection.

Free neuromast development and appearance of the lateral line canal system in the spotted bass preceded that of the northern hog sucker. This accelerated development of the spotted bass was related to earlier …

Review Of "Embryonic And Fetal Development, Reproduction In Mammals" Edited By C. R. Austin And R. V. Short, Scott F. Gilbert

Biology Faculty Works

No abstract provided.

Crystallization Of The Gene 45 Protein From The Dna Replication Fork Of Bacteriophage T4, C. Thaller, B. Alberts, E. Goldsmith, S. Sprang, R. Fletterick

Biological Sciences Faculty Publications

The gene 45 protein from bacteriophage T4 has been purified and is crystallized. This protein is part of the T4 DNA replication complex. The crystallized protein is active in complementation assays. X-ray diffraction analysis is in progress; data are measured for the native and several heavy atom derivatives. The crystals diffract to about 3.5-Å resolution.

Cloning The Gene For The Malolactic Fermentation Of Wine From Lactobacillus Delbrueckii In Escherichia Coli And Yeasts., Steven A. Williams, Robert A. Hodges, Terry L. Strike, Richard Snow, Ralph E. Kunkee

Biological Sciences: Faculty Publications

No abstract provided.

Financial Exigency: Need It Affect The Quality Of Biology Curricula?, Robert G. Thomson, Peter Abramoff

Biological Sciences Faculty Research and Publications

Declining enrollments and financial restraints require that science departments seek ways to meet academic commitments within the framework of reduced budgets and faculty resources without sacrificing quality programs. The following describes our evaluation of the role of the laboratory in the undergraduate biology curriculum and the positive effects achieved on our academic, financial, and faculty resources by separating labs from lecture courses and reducing the number of labs required for majors and nonmajors. Several years ago we experienced increased enrollments coupled with only modest increases in funds to deliver our undergraduate instructional programs. To resolve this problem we developed a …

Factors Affecting Nest Site Location In Gila Woodpeckers, Jerome J. Korol, Richard L. Hutto

Biological Sciences Faculty Publications

We investigated the locations of Gila Woodpecker (Melanerpes uropygialis) nest sites in Organ Pipe Cactus National Monument, Arizona. A significantly greater proportion of saguaro cacti in arroyos had woodpecker holes than did those on hillsides, ridgetops or desert flats. Saguaros used for nest-hole excavation were taller and more likely to have branches than randomly chosen saguaros. Both active and inactive nest holes were nonrandomly oriented, with the mean vectors of orientation being in northwesterly directions. Variation in nest-hole orientation probably is not due to seasonal differences in excavations because the orientation of spring active (late March) nest holes was not …