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Ankrd2 May Alleviate Denervation-Induced Skeletal Muscle Atrophy By Inhibiting Inflammation, Yalin Zhang, Lin Zhang, Li Li, Liqin Zhang, Li Zeng, Jiaying Qiu, Zhengrong Xi, Wenwen Wang

Turkish Journal of Biology

Background/aim: Sciatic nerve injury causes a loss of skeletal muscle innervation, reduced motor function, and eventual muscle atrophy. Inflammation and increased protein degradation are key factors contributing to muscle atrophy. Inflammation is activated early during muscle atrophy and can be modulated by various factors. However, the precise role of inflammation in denervation-induced muscle atrophy remains unclear. Materials and methods: Transcriptome sequencing was used to determine that the inflammatory response occurs early during denervation-induced muscle atrophy. RT-qPCR validation of several inflammatory factors showed rapid upregulation at early stages, followed by gradual downregulation. Weighted gene coexpression network analysis of differentially expressed genes …

Unusual Rna Polymerase Ii Patterns In Meiotic Prophase I In Nannospalax Xanthodon: Insights Into Chromatin, Telomeres, And Sex Chromosomes, Sergey N. Matveevsky, Ferhat Matur, Faruk Çolak, Andrei Kandaurov, Mustafa Sözen, Svetlana V. Pavlova, Oxana Kolomiets

Turkish Journal of Biology

Background: Meiotic transcription is a highly regulated process involving stage-specific chromatin remodeling and dynamic RNA polymerase II (RNAPII) activity. Nevertheless, our current understanding is largely based on classical model organisms, while many nonmodel mammals remain unexplored. This study investigates the transcriptional dynamics and chromatin features during meiotic prophase I in Nannospalax xanthodon, a subterranean blind mole rat species with extensive intraspecific karyotype variation.Materials and methods: Using immunofluorescence, we analyzed synapsis and transcriptional activity in spermatocyte nuclei of two geographically distant N. xanthodon males, detecting SYCP3, CREST, γH2AFX, and phosphorylated RNAPII (Ser5, Ser2). Telomereassociated proteins RAP1 and TERT were also examined …

Protective Effect Of Pinocembrin On Zearalenone-Induced Hepatotoxicity Via Pi3k/Akt And Nrf2 Signaling In Albino Mice, Sujatha Tejavat

Turkish Journal of Biology

Background/aim: Zearalenone (ZEA) is a Fusarium-derived mycotoxin that frequently contaminates food and feed and induces hepatotoxicity in humans and animals through oxidative stress and inflammation. Pinocembrin (PCM), a naturally occurring flavonoid, has potent antioxidant and antiinflammatory properties. This study investigated the hepatoprotective effects of PCM against ZEAinduced liver injury in albino mice, with a focus on the nuclear factor erythroid 2–related factor 2 (NRF2) and phosphoinositide 3-kinase/protein kinase B (PI3K/Akt) signaling pathways. Materials and methods: Twenty-four albino male mice were allocated into four groups: (1) control (vehicle 0.05% dimethyl sulfoxide), (2) ZEA-treated (40 mg/kg body weight), (3) PCM-treated (50 mg/kg …

Studies On The Contribution Of Osh6 To The Functions Of Late Endosomes, William King

Theses and Dissertations

The late endosome (LE) is a hub organelle connecting endocytic, biosynthetic, exocytotic, and autophagic vesicle traffic pathways. Malfunctions of LEs are associated with multiple age dependent diseases such as Parkinson’s disease (PD). Despite this significance, how cells refurbish lipids and proteins to LEs to maintain their sustained activities are not clear. Previous studies in the model organism budding yeast show that up-regulation of a lipid transporter Osh6 (PERG6-OSH6) accumulates phosphatidylinositol-4-phosphate (PI4P) on the trans-Golgi network. It also promotes vacuole fusion and maintains the robustness of vacuoles in aged cells. Since LEs are the organelle between the TGN and vacuoles in …

Skin Pattern As A Potential Monitoring Tool For Frog Populations, Lídize Sagarnaga Rivera, Virginie Rolland Dr., Lorin Neuman-Lee Dr.

Create@State

Skin pattern as a potential monitoring tool for frog populations

Population monitoring, which requires identifying individuals, is crucial for species conservation. Common techniques involve marking, which can harm animals and alter their behaviors. Natural markings are an alternative not yet explored for amphibians. Yet, the traditional method with elastomers (fluorescent implants) is invasive and not completely reliable—the tag may become lost or hard to see. The American green tree frog (Dryophytes cinereus) possesses naturally occurring and distinct golden dots on their dorsal side.  Therefore, we aim to assess whether patterns of golden dots on the skin of green …

Amyloid Beta-42 Neurotoxicity In Drosophila Retinal Development, Shay O. Sims, Pierce Duncan, Malachi Abon, Jacob G. Smith

Create@State

Alzheimer's disease (AD) represents one of the most prevalent neurodegenerative disorders globally. Thus far, the underlying mechanisms associated with the pathogenesis of AD are innately elusive. Two hallmarks associated with the progression of AD exist, including Amyloid-Beta 42 (Aβ42) protein plaque formation and hyperphosphorylation of tau proteins in neurons. Amyloid-beta is known to concentrate with other neuronal proteins as complexes form, including the cytoskeletal protein actin. However, whether actin contributes to neuronal decline in AD is unknown. To further understand the cellular cascades associated with AD, we investigated the role of cytoskeleton dynamics in modulating oxidative stress and neuroinflammation within …

Variation In Infection Prevalence Of Splendidofilaria Pectoralis Across Alaskan Grouse Populations, Anahi Garcia Valdez, Diego Martinez

Create@State

In recent years, rapid climate change has altered species distributions worldwide and influenced parasite transmission dynamics in wildlife systems. Parasite infections play an important role in shaping patterns of disease distribution across species and populations. Understanding variation in infection prevalence across geographic regions and host demographic groups is essential for interpreting these patterns. Splendidofilaria pectoralis is a filarial nematode that infects the connective tissues of grouse (Aves: Tetraonini) in Alaska and has been moving northward as the climate warms. However, relatively little is known about the variation in prevalence of the nematode across geography and demography. This project examines variation …

Inc3vits Model: A Hybrid Architecture To Accelerate And Reduce Complexity For The Deepvariant Model For Variant Calling, Mustafa Al-Saffar, Sura Z. Al Rashid

Karbala International Journal of Modern Science

Deep learning has revolutionized genomic variant calling, yet the computational cost of current systems continues to limit scalability. We present a controlled efficiency study of DeepVariant-style pileup architectures under identical training and inference conditions, comparing architectural downsizing with a hybrid CNN–local attention design. Inc3ViTs pairs a streamlined InceptionV3 stem with a lightweight local attention head based on patch tokenization and windowed self-attention, enabling a direct comparison with a CNN-only reduced Inception baseline. Across whole-genome and whole-exome short-read datasets, Inc3ViTs reduces training time by ~40–50% and reduces inference runtime relative to the original DeepVariant. The CNN-only baseline indicates that most speedups …

Elasmobranchii Oral Microbiome Diversity Within Southeast Florida: Implications Of Shark Zoonotic And Antibiotic Resistant Pathogens Associated With Human Interactions, Amanda Finnefrock

All HCAS Student Capstones, Theses, and Dissertations

Sharks are incredibly common in the Atlantic and Gulf waters surrounding Florida. Despite the prevalent nature of these animals, little research exists on the composition of their microbial communities (or “microbiomes”). Here we aim to determine the microbial makeup of the oral environment of sharks so that we can determine if there are any bacteria of clinical significance to humans, such as being antibiotic-resistant or having elevated capability for pathogenicity. A total of 41 samples were collected from the mouths and teeth of bull, Caribbean reef, great hammerhead, lemon, nurse, and sandbar sharks caught in Southeast Florida using sterile swabs …

Geographical And Anatomical Microbiome Variation In Invasive Green Iguanas (Iguana Iguana), Amanda A. Diaz

All HCAS Student Capstones, Theses, and Dissertations

The common green iguana (Iguana iguana) was introduced to South Florida in 1965 and has since been an especially pernicious threat both environmentally and economically. This highly invasive species is also expanding in range and populations at an astonishing rate. Understanding how this reptile is able to adapt and thrive in novel environments is of imperative need in order to begin managing their disruptive populations. Notwithstanding their negative impacts and significance, the microbiome of wild populations has not yet been characterized. Here, using 16S ribosomal RNA gene sequencing, this study is the first to characterize the microbiome composition …

Mental Gymnastics: How Misconceptions Drive Unstable Forms Of Scientific And Religious Reconciliation, Jamie Jensen, Jade Sorensen, Andrea Phillips, Mackay Strong, Isaac A. Woolley, Spencer M. Johanseon, Afton Fillmore, Luke N. Noakes

ScholarsArchive Data

Interview transcripts of introductory biology students attempting to reconcile evolutionary science with religious faith.

Potential Applications Of Thymus Vulgaris In Dental Care: A Review Of Antimicrobial And Oral Health Benefits, Lakenzia Z. Ham

Undergraduate Research Conference

This research proposal focuses on how common thyme (Thymus vulgaris) can be used in dental care. There has been more interest in natural products for dental health because some common dental treatments can have side effects or lead to antibiotic resistance. Thyme contains natural compounds like thymol and carvacrol that help kill bacteria, reduce inflammation, and prevent buildup on teeth.

The purpose of this study is to investigate the current scientific literature to see the effectiveness of thyme in preventing common oral health problems like cavities, gum disease, and bad breath. Research shows that thyme can fight bacteria …

Late-Season Forb Availability And Bumble Bee Foraging Patterns In Iowa’S Restored Prairies And Suburban Gardens, Isaac De Young, Ellary Carson, Mya Cole, Tucker Draayer, Sharon Huffman, Naomi Johnson, Jasmine Lux, Genesis Maldonado, Caitlin Olson, Maelee Rodenburg, Jordan Shull, Travis Sweeney, Arianna Van Donkelaar, Laurie Furlong, Todd Tracy

Celebration of Research

Pollinators are essential to terrestrial ecosystems, and bumble bees are among the most effective pollinators in the United States (Hatfield et al., 2012). They pollinate forbs (non-grass herbaceous flowering plants), including crops, garden plants, and native prairie species (Dumroese et al., 2016). Their ability to travel long distances, forage in cool temperatures, and buzz-pollinate makes them critical to both wild and agricultural productivity (Mola & Williams et al., 2019). Research shows that bumble bee populations have declined in recent decades due to habitat loss, reduced floral resources, pesticide exposure, and climate change (IPBES, 2016).

Northwest Iowa is dominated by agriculture, …

Eagleeye Is Landing: Discovery Of Cytotoxic And Defense Genes In A Cluster A16 Mycobacteriophage, Clara Bader, Kylie Foss, Mallory Gramstad, Korver Hupke, Jenna Kluxdal, Elsie Larson, Ayda Maassen, Adriana Sandbulte, Dane Schoenborn, Emma Steinhardt, Alexa Trover, Sara S. Tolsma

Celebration of Research

EagleEye is a temperate bacteriophage, which was isolated on the host, Mycobacterium smegmatis mc2 155 from soil collected in Houston, TX. It is classified as cluster A16. We are using a systematic genetic screen to uncover functions of phage genes for which the function is unknown. We cloned each gene into the pExTra plasmid and assayed phage gene individually for two phenotypes: cytotoxicity, the ability to interfere with host cell growth, and defense, the ability to protect the host cell from infection by other phages. We have cloned all but 15 of EagleEye’s 95 protein-coding genes. Thus far, we have …

If You Give A Mouse A Phage, Peyton Abraham, Princess Bola-Lawal, Amelia Brown, Anne Eveland, Jenna Kluxdal, Elsie Larson, Gabrielle O'Bannon, Leonardo Romo, Sara S. Tolsma

Celebration of Research

Island3 (Cluster I1) and Sbash (Cluster I2) are Mycobacteriophages that have a high degree of genetic similarity—especially in the region of their genomes that include genes for structural proteins. Because their structural proteins appear so similar, we hypothesized that antibodies raised against Island3 would cross-react with Sbash and vice versa. To test our hypothesis, we immunized female BALB/c mice with phage high titer lysates or filtered M. smegmatis (control), collected serum, and used the antibodies in ELISA and Western Blot assays. We observed less cross-reactivity than we expected.

Annotation Of Bacteriophages Redraider89, Superherocarly, Cherrycola, And Kouch, Brianne Brandt, Mya Cole, Matthew Dawkins, Julia De Vos, Isaac De Young, Hannah Gordon, Landon Greve, Brody Hoverstein, Mia Kellenberger, Genesis Maldonado, Charlotte Pritchard, Noah Robinson, Leonardo Romo, Mackenzie Schram, Grace Timm, Arianna Van Donkelaar, Kamea Van Kalsbeek, Byron Noordewier, Sara S. Tolsma

Celebration of Research

Phages are important research tools and can be used as an alternative to antibiotics. They are abundant and diverse. Genome annotation is used to better understand phage diversity as well as individual phages for use in the lab and clinic. We annotated the genomes of four novel phages: RedRaider89, SuperHeroCarly, Kouch, and CherryCola. All four phages were discovered by Northwestern College students. CherryCola is a Cluster DC1 phage that infects Gordonia terrae. It has 92 predicted protein-coding genes. RedRaider89 (Cluster B1) and SuperHeroCarly (Cluster D1) infect Mycobacterium smegmatis mc²155. RedRaider89 has 99 predicted protein-coding genes and SuperHeroCarly has 87 …

Understanding The Role Of Lipid Metabolism On Pancreatic Cancer Metastasis, Luke Fischer, Kylie Foss, Jacob Lewis, Joshua Lewis, Emma Steinhardt, Megan Vangarven, Cody Rozeveld

Celebration of Research

Pancreatic cancer is one of the deadliest types of cancer due to late-stage detection and early metastasis. Other than smoking, one of the few known risk factors for pancreatic cancer is obesity (1). On the cellular level, excess fat can promote cell migration and invasion (2). However, the exact fat utilization pathway remains unknown. We propose that lysosomes may be exploited in pancreatic cancer to break down fat and provide energy for metastasis and invasion.

Bacterial 2-Hybrid Assay: Interactions Of Phage Island3'S Gene 36 And Mycobacterium Smegmatis Gene Products, Elsie L. Larson, Sara Tolsma

Celebration of Research

Island3 is a Cluster I1 bacteriophage that infects Mycobacterium smegmatis. Northwestern College students are performing systematic genetic screens to identify Island3 genes that are cytotoxic or protect host cells from infection by other phages (defense). We identified Island3’s gp36 as a cytotoxic gene. Now we are using a bacterial two-hybrid assay to identify a host protein(s) interaction partner. We hypothesize that host proteins that bind gp36 will reveal the mechanism by which gp36 confers cytotoxicity.

Investigation Of Northwest Iowa Pollinator Communities Using Two Survey Methods, Ellary Carson, Mya Cole, Isaac De Young, Sharon Huffman, Naomi Johnson, Genesis Maldonado, Maelee Rodenburg, Travis Sweeney, Tark Griebel, Laurie Furlong, Todd Tracy

Celebration of Research

The ecological services of insect pollinators are well established and the documented decline in pollinator biodiversity is of great concern. Baseline data and continued monitoring are essential as we ongoing trends in pollinator communities and evaluate efforts supporting their recovery. Using two survey methods, our study provides pollinator community data at two conservation sites in Northwest Iowa. In September of 2025, we used three colors of pan traps (blue, yellow, and white) to investigate pollinator communities at a small, reconstructed prairie (Buena Vista County Park) and a nearby partially restored native prairie (The Nature Conservancy Glacial Hills Preserve). We also …

The Impact Of Sex Education On College Student’S Perceptions Of Sexual Taboos, Noel Gamon

Honors Theses

The purpose of this research is to explore how college students' differing levels of sex education are associated with their views about sexual behavior, particularly behaviors defined as taboo. In comparison to 25 years ago, adolescents are less likely to receive important sex education today; and are unlikely to receive valuable information until after their first sexual experience. Conventionally, the benefits of a sex education program are linked with its ability to lower STI rates, unplanned pregnancies, unwanted behavior, etc. Sexual taboos are sexual practices excluded from the hegemonic heterosexual monogamous relationship model. Presently, society acknowledges a wider range of …

G1p3 Dysregulates Interferon-Stimulated Gene Expression In Er (+) Breast Cancer Cells, Karina Santana

Honors Theses

Even with advances in diagnosis and treatment, breast cancer remains the second leading cause of cancer-related death in women, with nearly 90% of fatalities linked to drug resistance driven by metastasis and recurrence. G1P3, an anti-apoptotic mitochondrial protein, is upregulated in breast cancer and has been associated with increased metastatic potential and poor prognosis. Recent findings show that G1P3 enhances interactions between Rab5-containing endosomes (RCEs) and mitochondria, and emerging evidence suggests that mitochondria play a critical role in initiating interferon (IFN) responses. Based on this, we hypothesized that G1P3-mediated interactions between RCEs and mitochondria promote interferon-stimulated gene (ISG) expression in …

Secondary Contact Dynamics In White-Breasted Nuthatches (Sitta Carolinensis) Across The Pine Ridge Of Nebraska, Eugene A. Huryn

Biology Theses, Dissertations, and Student Creative Activity

Populations’ interactions in contact zones are important for understanding divergence dynamics of closely related taxa. Such interactions may vary from inter-population divergence being reinforced to secondary contact resulting in the creation of a hybrid swarm. The ecology of each lineage, and different factors like behavior and ecological niches, may impact the outcome of such contact. However, there is little current research on how habitat choice at local scales might reflect distinct populations, particularly in cryptic species. To study how niche dynamics affect diversification, we studied the White-breasted Nuthatch (Sitta carolinensis) complex in northwestern Nebraska. Two members of this …

Neural Signaling Contributes To Heart Formation And Growth In The Invertebrate Chordate, Ciona Robusta, Hannah N. Gruner, C. J. Pickett, Jasmine Yimeng Bao , '24, Richard Garcia , '24, A. Hozumi, T. D. Scully, Sydney Popsuj, Shaoyang Ning, Mavis Gao , '26, Gia Bautista , '23, Keren Meze , '24, Haeun Karissa Lim , '25, T. Osugi, Mae Collins-Doijode , '26, Ofubofu Cairns , '24, Gabriel Levis , '23, Shu Yi Chen , '24, Taixi Gong , '24, H. Satake, A. M. Klein, Y. Sasakura, Bradley Justin Davidson

Biology Faculty Works

Neurons contribute to the complex interplay of signals that mediate heart development and homeostasis. Although a limited set of studies suggest that neuronal peptides impact vertebrate heart growth, the specific contributions of these peptides to cardiomyocyte progenitor differentiation or proliferation have not been elucidated. Here, we show that the neuropeptide tachykinin along with canonical Wnt signaling regulate cardiomyocyte progenitor proliferation in the chordate model Ciona robusta. In C. robusta, the heart continues to grow throughout adulthood and classic histological studies indicate that a line of undifferentiated cells may serve as a reserve progenitor lineage. We found that this line of …

The Efficacy Of Manganese Sulfate As A Negative Contrast Agent For Magnetic Resonance Cholangiopancreatography, Zainab Abdulla Mankhi

Karbala International Journal of Modern Science

The current study aims to find an alternate oral contrast media for use in magnetic resonance cholangiopancreatography (MRCP) that satisfies the following criteria, the greatest imaging quality safety, no or few side effects, and low cost. The present study created an oral contrast agent sample (solution) by dissolving a one tablet of manganese sulfate supplement (taken daily dose) in 200 ml of distilled water. Thirty-three volunteers assessed the sample using MRCP examine pre and post contrast. By evaluating the signal intensity to compute contrast (C), signal to noise ratios (SNR), and contrast to noise ratio (CNR), the resulting MR images …

Antioxidant And Antifungal Activity Of Lactuca Serriola L. Plant And Silver Nanoparticles Against Aspergillus Species., Sakar Abdulqadr Saheed, Badr Qader Ismael

Karbala International Journal of Modern Science

Medicinal plants remain a valuable source of antifungal agents, and recent developments in green nanotechnology have increased the prospects for Lactuca serriola L. as a useful bioresource. In the current study, leaves and roots were extracted by ultrasonic-assisted chloroform, ethanol, and aqueous extraction methods for the biological mediation of silver nanoparticles (Ag NPs). Screening of Phytochemicals for extracts revealed the present secondary metabolites. Ag NPs were verified through UV-visible spectroscopy, indicating a peak at (408-423 nm) corresponding to a surface plasmon resonance, FT-IR functional groups of O–H, C=O, and C–N binding. The morphological analysis revealed an irregular-to-quasi-spherical shape, with an …

Roles Of Mads-Box Transcription Factors In Plant Responses To Abiotic And Biotic Stresses, Na Liu, Zihao Liu, Guangxiang Tian, Shijia Zhao, Hao Chu, Yiheng Hu, Yingfan Zhao, Yanyan Zhang, Kun Cheng, Daowen Wang, Wenming Zheng, Zhengqing Fu, Xu Wang

Faculty Publications

Plants deploy sophisticated adaptive mechanisms to mitigate the detrimental effects of abiotic stresses (drought, salinity, temperature extremes, and heavy metals) and biotic stresses (pathogens and senescence) on growth and productivity. Central to these responses are transcription factors (TFs) that orchestrate stress-responsive gene networks. Among TF families, MADS-box proteins, characterized by their evolutionarily conserved DNA-binding domain, function as pivotal regulators of developmental plasticity and stress adaptation. While recent advances have clarified their roles in abiotic stress tolerance, a systematic integration of their functions in biotic stress responses has yet to be achieved. This review synthesizes current knowledge on how MADS-box TFs …

Testing Effects Of Acoustic Propagation, Morphology, And Intrasexual Selection On The Elaboration And Performance Of Song In Bachman’S Sparrows (Peucaea Aestivalis), Hans Kayser Gonzembach

Electronic Theses and Dissertations

Birdsong is a communication signal shaped by ecological, morphological, and cultural processes. While many songbird species produce relatively simple vocal repertoires, others, such as the Bachman’s Sparrow (Peucaea aestivalis), produce moderately large repertoires with multiple distinct and context-dependent song categories. Despite this complexity, little is known about the major drivers that shape song elaboration in this species. Additionally, it is unclear whether abiotic factors play a significant role in determining how different song types are learned and shared within a population. To better understand how ecological and social pressures shape song variation, I conducted three studies using Bachman’s …

Assessing Walleye Recruitment Via Otc Marks And Temporal Sampling Changes In Smallmouth Bass Population Structure In Lake Mcconaughy, Nebraska, Robert D. Allison Iv

Biology Theses, Dissertations, and Student Creative Activity

Lake McConaughy is a socially and economically important reservoir managed to provide diverse, high-quality angling opportunities. Walleye are the most frequently targeted species, although Smallmouth Bass have increased in popularity in recent years due to improvements in abundance and size structure. Walleye have been stocked annually since 1989 to support the population and have been the focus of extensive research. In contrast, Smallmouth Bass have been present for over 80 years and are self-sustaining; however, their population has not been formally evaluated since introduction.

The first objective of this study was to develop an oxytetracycline (OTC) mark retention correction factor …

Studying The Role Of Combitional Flavonoids Against Toxicity Of Chemothrapeutic Drugs In Human Fibroblast Cells, Hussain Al Zouabi

Thesis/ Dissertation Defenses

Chemotherapy remains the primary treatment strategy for cancers; however, its clinical efficacy is often limited by severe toxic effects resulting in normal cells. The study investigated the protective potential of the flavonoids epigallocatechin-3-O-gallate (EGCG) and quercetin, used in combination, against chemotherapy-induced toxicity in human fibroblast F-180 cells. Cells were exposed to three commonly used chemotherapeutic agents, doxorubicin, cisplatin, and etoposide, in the presence or absence of EGCG and quercetin at concentrations of 25 and 50 μM. cells viability was assessed using the MTT assay, while oxidative stress and antioxidant capacity were evaluated by intracellular reactive oxygen species (ROS) and total …

Exploring Patterns Of Microhabitat Use Among Darters In The Lower Little River Watershed, Arkansas, Nate J. Martin, Savannah L. Wise

ATU Scholars Symposium

In this study, we seek to explore patterns of microhabitat use among darters, including variables of stream depth, water velocity, substrate composition, aquatic vegetation, and woody debris cover. The most informative microhabitat variables will be assessed using nonmetric multi-dimensional scaling (NMDS). Species with more than five individuals collected were included in analysis, totaling eight species across 24 sites in the Lower Little River Watershed of southwest Arkansas. We caught 28 Plains Darters (Etheostoma pulchellum), 27 Dusky Darters (Percina sciera), 21 Orangebelly Darters (Etheostoma radiosum), 16 Channel Darters (Percina copelandi), 13 Harlequin Darters (Etheostoma histrio), 11 Common Logperch (Percina caprodes), 7 …