Digital Commons Network^™

Understanding The Role Of Serine 26 Phosphorylation Changes In Peroxidemediated Trafficking Of System XC- To The Cell Membrane, Katherine Lane

23rd Annual A. Paul and Carol C. Schaap Celebration of Undergraduate Research and Creative Activity (2024)

Composed of xCT and 4F2HC, System x_c^- is involved in importing cystine and exporting glutamate from the cell. xCT is directly involved in cystine-glutamate transport, and 4F2hc is responsible for stabilizing and moving xCT onto the cell membrane. Previous studies found that phosphorylating serine 26 (S26) on xCT inhibits xCT activity by moving system x_c^- off the cell membrane into lysosomes to be degraded (Figure 1). A previous study by the Chase lab showed that treating COS7 cells with hydrogen peroxide (H2O2) led to significantly higher amounts of xCT on the cell membrane. The objective of …

Investigating Auditory And Visual Seasonality In House Finches, Ashley Lauraine, Hannah Peterson, Jordan Winters

23rd Annual A. Paul and Carol C. Schaap Celebration of Undergraduate Research and Creative Activity (2024)

Many birds receive both visual and auditory signals in mate selection, such as plumage color and song differences within conspecifics, which has implications for behavioral and communication processes. This processing of stimuli from different systems as a combined signal is also known as multimodal sensory processing. Sex hormone levels correlating with reproduction efforts vary seasonally in temperate animals, leading to changes in sensory processing as well. As the seasons progress, sex hormones (i.e. estrogen and testosterone) fluctuate, and an increase or decrease in these hormones has shown an impact on hearing in songbird species, however, less is known about the …

Implications Of Iron Oxide Nanoparticle Exposure On The Auditory Physiology And Iron Bioaccumulation In House Sparrows (Passer Domesticus), Shae Johnston, Peyton Hallemann, Olivia Sprys-Tellner

23rd Annual A. Paul and Carol C. Schaap Celebration of Undergraduate Research and Creative Activity (2024)

Air pollution (including particulate matter, PM) has increased as a result of a dramatic increase in urbanization. Nanoparticles within PM can be detrimental to health when inhaled., because they are small enough to bypass the blood-brain and blood-gas barriers, and can potentially accumulate in organs within the organism. For example, previous work has linked bioaccumulation of iron oxide nanoparticles (IONPS) to cancer and organ poisoning. Interestingly, in model organisms, nanoparticle exposure has also been linked to hearing deficits, suggesting that decline in hearing may be an early bioindicator of future health consequences. While the specific mechanism for this hearing deficit …

Explorehope Summer Camps: Amazing Animal Abilities, Hannah Sturgeon, Alyssa Archer, Ashley Lauraine

23rd Annual A. Paul and Carol C. Schaap Celebration of Undergraduate Research and Creative Activity (2024)

Did you know that mice can hear sounds that humans cannot? Or that birds and bees can see colors we can only dream of? ExploreHope is an organization on the Hope College campus that has been hosting summer camps for elementary to high school level students for 27 years. The ExploreHope Amazing Animal Abilities camp for students in grades 6 through 9 will explore the many incredible sensory abilities of animals. This camp will teach participants about a vast variety of sensory systems observed across the animal kingdom including the visual, auditory, and olfactory systems, mechanoreception and kinesthetics, and the …

The Effects Of Vasopressin On Aquaporin-1 Expression And Localization In The Presence And Absence Of Vacm-1/Cul5, Parker Friend, Rachel Amicucci

23rd Annual A. Paul and Carol C. Schaap Celebration of Undergraduate Research and Creative Activity (2024)

The vasopressin-activated calcium mobilizing (VACM-1/Cul-5) protein plays a role in the E3 ubiquitin ligase system which is involved in the destruction of specific proteins. Our focus is on the impact that the (VACM-1/Cul-5) protein has on other proteins' expression and localization within human umbilical vein endothelial cells (HUVEC). One of the proteins of interest to us is aquaporin-1 (AQP1). AQP1 is a water transporter that impacts cell growth and angiogenesis. To evaluate how the presence of VACM-1/Cul5 impacts AQP1, cells with and without the VACM-1/Cul5 gene are analyzed in the presence of increased vasopressin. VACM-1/Cul5 is knocked out of the …

Hormetic Modulation Of On Mtor Mitochondria Cross Talk For Mitigation Of Cellular Aging, Mahalakshmi R

Theses and Dissertations

Cellular aging is a gradual process involving structural and functional deterioration of cells. Mitochondria, the biological clock in cells, are known to play a crucial role in the progression of cell aging. According to the mitochondrial free radical theory of aging, oxidative phosphorylation in the mitochondria produces free radicals, such as, reactive oxygen species (ROS), which lead to oxidative stress in cells. This, in turn, results in mitochondrial dysregulation, and eventually causes degeneration of cellular components, thereby propagating cell aging. However, it is to be underscored that aging, at its core, is a multifaceted phenomenon that involves a number of …

Evaluation Of Seizure Recognition And First Aid Training Course And Improving Self-Efficacy Related To Medical Emergencies In Mississippi, Anna Katherine Herrington, Collin Earl Wayne Crawson, Daniel Robert Zuckerman

Honors Theses

Within recent years, there has been increased attention to the growing physician shortage across the United States. This shortage has placed substantial strain upon regions of lower healthcare access, particularly those of rural regions such as much of the state of Mississippi. As rural healthcare providers are placed under more strenuous conditions, they encounter issues of increased patient volume, lack of a trauma team, or adequate transportation to higher-level care facilities. These issues may exacerbate the present inequities in cost of living, access to adequate healthcare, and the distress caused by these concerns. Individuals with epilepsy are one cohort with …

Influence Of Rna Circularity On Target Rna-Directed Microrna Degradation, Federico Fuchs Wightman, Jerónimo Lukin, Sebastián A Giusti, Michael Soutschek, Laureano Bragado, Berta Pozzi, María L Pierelli, Paula González, Juan P Fededa, Gerhard Schratt, Rina Fujiwara, Jeremy E Wilusz, Damián Refojo, Manuel De La Mata

Faculty, Staff and Students Publications

A subset of circular RNAs (circRNAs) and linear RNAs have been proposed to 'sponge' or block microRNA activity. Additionally, certain RNAs induce microRNA destruction through the process of Target RNA-Directed MicroRNA Degradation (TDMD), but whether both linear and circular transcripts are equivalent in driving TDMD is unknown. Here, we studied whether circular/linear topology of endogenous and artificial RNA targets affects TDMD. Consistent with previous knowledge that Cdr1as (ciRS-7) circular RNA protects miR-7 from Cyrano-mediated TDMD, we demonstrate that depletion of Cdr1as reduces miR-7 abundance. In contrast, overexpression of an artificial linear version of Cdr1as drives miR-7 degradation. Using plasmids that …

Structural Basis Of Dna Crossover Capture By Escherichia Coli Dna Gyrase, Marlène Vayssières, Nils Marechal, Long Yun, Brian Lopez Duran, Naveen Kumar Murugasamy, Jonathan M Fogg, Lynn Zechiedrich, Marc Nadal, Valérie Lamour

Faculty, Staff and Students Publications

DNA supercoiling must be precisely regulated by topoisomerases to prevent DNA entanglement. The interaction of type IIA DNA topoisomerases with two DNA molecules, enabling the transport of one duplex through the transient double-stranded break of the other, remains elusive owing to structures derived solely from single linear duplex DNAs lacking topological constraints. Using cryo–electron microscopy, we solved the structure of Escherichia coli DNA gyrase bound to a negatively supercoiled minicircle DNA. We show how DNA gyrase captures a DNA crossover, revealing both conserved molecular grooves that accommodate the DNA helices. Together with molecular tweezer experiments, the structure shows that the …

Akap12 Upregulation Associates With Pde8a To Accelerate Cardiac Dysfunction, Hanan Qasim, Mehrdad Rajaei, Ying Xu, Arfaxad Reyes-Alcaraz, Hala Y Abdelnasser, M David Stewart, Satadru K Lahiri, Xander H T Wehrens, Bradley K Mcconnell

Faculty, Staff and Students Publications

BACKGROUND: In heart failure, signaling downstream the β2-adrenergic receptor is critical. Sympathetic stimulation of β2-adrenergic receptor alters cAMP (cyclic adenosine 3',5'-monophosphate) and triggers PKA (protein kinase A)-dependent phosphorylation of proteins that regulate cardiac function. cAMP levels are regulated in part by PDEs (phosphodiesterases). Several AKAPs (A kinase anchoring proteins) regulate cardiac function and are proposed as targets for precise pharmacology. AKAP12 is expressed in the heart and has been reported to directly bind β2-adrenergic receptor, PKA, and PDE4D. However, its roles in cardiac function are unclear.

METHODS: cAMP accumulation in real time downstream of the β2-adrenergic receptor was detected for …

Lead Compound Development Of Src-3 Inhibitors With Improved Pharmacokinetic Properties And Anticancer Efficacy, Dong Lu, Jianwei Chen, Li Qin, Imani Bijou, Ping Yi, Feng Li, Xianzhou Song, Kevin R Mackenzie, Xin Yu, Bin Yang, Sandipan Roy Chowdhury, James D Korp, Bert W O'Malley, David M Lonard, Jin Wang

Faculty, Staff and Students Publications

Steroid receptor coactivator 3 (SRC-3) is a critical mediator of many intracellular signaling pathways that are crucial for cancer proliferation and metastasis. In this study, we performed structure-activity relationship (SAR) exploration and drug-like optimization of the hit compound SI-2, guided by in vitro/in vivo metabolism studies and cytotoxicity assays. Our efforts led to the discovery of two lead compounds, SI-10 and SI-12. Both compounds exhibit potent cytotoxicity against a panel of human cancer cell lines and demonstrate acceptable pharmacokinetic properties. A biotinylated estrogen response element (ERE) pull-down assay demonstrated that SI-12 could disrupt the recruitment of SRC-3 and p300 in …

Chemical-Mediated Mate Attraction In Clover Root Curculio; Sitona Hispidulus (F.), A Below Ground Pest, Kristin Leutgeb

Student Research Symposium

Few control options

• Registration of broad-spectrum soil fumigant insecticides discontinued
• Effective monitoring is time- and resource-intensive

Crop damage from feeding on root nodules by soil-dwelling CRC larvae

• Nodules are a symbiotic relationship between the plant and rhizobia, which provide nitrogen to the plant host
• Curculionid beetles can sense the nodules

Mating by pheromones. CRC produces contact and volatile chemicals for mating and egg laying (oviposition).

• Utilize pheromone during active mating above ground

Effects Of Fungal Biopesticide Doses On Mortality In Bumble Bees, Colton Burris

Undergraduate Research Symposium

Agricultural practices often include the use of pesticides to produce meaningful crop yields. While the pesticide may be used to target “pest” insects (e.g. thrips, aphids, whiteflies), it can also have negative effects on important pollinating insects such as bees. Neonicotinoid pesticides have been found responsible for widespread decline in bee biodiversity, and were even banned in Europe (Sgolastra et al. 2020) . Beauveria bassiana, sold as Botanigard, on the other hand, is a fungal biopesticide that is marketed to be a safer alternative for bees, and has been found to have other possibly harmful effects to the inner …

Competition Or Prey: C. Elegans Populations In The Presence Of P. Pacificus, Charles Greene, Elena Zobel, Andrea Scharf

Undergraduate Research Conference at Missouri S&T

The predatory and territorial behaviors of the model organism P. pacificus provides a useful opportunity to study territorial defensive behaviors and optional predatory behaviors in relation to availability of preferred food sources. P. pacificus will be grown with C. elegans in shared cultures, in which E. coli will be placed and used to feed both species of worm. The number of predation events and territory defense events by P. pacificus against C. elegans will be recorded and tracked at differing levels of food concentrations. One potential issue is the different mouth morphs found in P. pacificus, with the stenostomatous (in …

A New Genetic Tool For Quorum-Sensing Ba, Amelia Markwell

Undergraduate Research Conference at Missouri S&T

This current research project focuses on the use of cell-to-cell communication in Bacterial Strain competition, specifically in soybean plants. Soybean plants are a high-yield crop produced here in the midwest, bringing in more than 8 billion dollars in monetary output for our state. The lab work focuses on one specific bacteria strain farmers commonly find in soybeans; this is called Bradyrhizobium japonicum. This nitrogen-fixing bacteria improves plant growth drastically. Cells have a way of communicating with each other in order to share information and adjust gene regulation accordingly. This is known as quorum sensing. My research last year focused on …

Modality's Effect On Aesthetic Ratings Of Poetry, Jessica Frame

Undergraduate Research Conference at Missouri S&T

Previous research has shown that vividness of evoked imagery is the strongest contributor to aesthetic pleasure for poetry. However, it is 114 unknown whether modality of the poem has an effect on the relationship between vividness and aesthetic appeal, as past work has only used written poems. In this study we want to evaluate rating of vividness, valence, arousal, and aesthetic appeal based on the modality in which poems are presented (listening only, reading only, or combined). Participants were randomly assigned to one of the three modalities: text-only (N=81 ), audio-only (N=74), or combined audio/text (N=78). Our results showed that …

Biosensors For Antibacterial Medical Applications, Ryan Fagan, Shelby Wallen

Undergraduate Research Conference at Missouri S&T

Healthcare associated infections are significant concern in medicine and as antibiotics are becoming less effective, we need new alternative methods to treat them. We are testing various materials that can be used to prevent infections in healthcare settings and in wound treatments. These materials include bioactive glass and modified hydrogel materials. From these studies we've learned these are effective in killing bacteria and preventing infections. The goal of this project is to determine the mechanism at which these materials kill bacteria. Our approach is to construct biosensor strains that can indicate bacterial stress response to antimicrobial agents. We have used …

Molecular Genetics And Phylogeny Of Bigmouth Shiner, Notropis Dorsalis, James Ramette, Patrick Appiah, Shila Koju, Jacqueline Kluba, Heath St, Dennis, Christian Bigler, Robert Hrabik, David D. Duvernell

Undergraduate Research Conference at Missouri S&T

The study of North American freshwater ichthyofauna reveals a diversity shaped by the continent's geologic past and the extensive effects of Pleistocene glaciation on river systems. Ericymba dorsalis (Bigmouth 69 Shiner) is a minnow species with a broad distribution in the Mississippi River basin from Wyoming to New York. Despite exhibiting consistent morphological features across its range, the species' widespread northern distribution suggests the potential existence of multiple, genetically distinct lineages. This research tested the hypothesis that separate drainage systems harbor genetically distinct groups of E. dorsalis. Our results identified five clades, each associated with major drainages. Populations of the …

Microbial Matrix: Genetic And Chemical Aspects Of Cell-Cell Communication, Amelia Markwell, Sophia Nicolette Militante

Undergraduate Research Conference at Missouri S&T

This research project focuses on looking at different quorum-sensing positive strains in bradyrhizobium japonicum, a species of legume-root bacteria found in soybean plants across the Midwest. Soybean plants are a high-yield crop produced here in the midwest, bringing in more than 8 billion dollars in monetary output for our state, making it Missouri's economic engine. Our objective, is to use different chemical testing methods to distinguish genetic differences between negative strains. This can be done through polymerase chain reaction, gel electrophoresis, and gene editing. Our goal has been to PCR amplify a fine-tuned promoter then clone it into the gene. …

The Influence Of Sensory Modality On Aesthetic Judgments Of Poetry, Jessica Frame, Kendra Mehl

Undergraduate Research Conference at Missouri S&T

Previous research has shown that vividness of evoked imagery is the strongest contributor to aesthetic pleasure for poetry. However, it is 19 unknown whether modality of the poem has an effect on the relationship between vividness and aesthetic appeal, as past work has only used written poems. In this study we want to evaluate rating of vividness, valence, arousal, and aesthetic appeal based on the modality in which poems are presented (listening only, reading only, or combined). Participants were randomly assigned to one of the three modalities: text-only (N=81 ), audio-only (N=74), or combined audio/text (N=78). Our results showed that …

Phytoremediation Of Nonpoint Source Pollution, Tylor Cheatham

Undergraduate Research Conference at Missouri S&T

Stormwater can bring high concentrations of nutrients into urban ponds resulting in eutrophic conditions and subsequent algal blooms. These 91 blooms can lead to hypoxic water that will ultimately cause fish deaths and loss of recreation. Often, the majority of urban stormwater flows into local ponds through an inlet, which provides a target area for nutrient removal methods. One method being explored by researchers is the use of floating treatment wetlands (FTWs). FTWs are floating mats with plants anchored to them, allowing the roots to be suspended in the water column for nutrient removal. Our previous research focused on optimizing …

Silent Bystanders Facing Bias. Is Silence Always Harmful, Sophie Firle

Undergraduate Research Conference at Missouri S&T

In STEM, women face bias that lowers their sense of belonging, identity safety, and self-esteem. These biases often occur in daily interactions with male peers in STEM, such as subtly sexist comments that doubt women's competence in STEM. A previous study that we conduction titles "Effects of Ally Confrontation on Target Outcomes" tested whether a subtly sexist comment that was confronted by a male ally would help increase women's sense of belonging, identity safety, and self-esteem relative to when a male bystander remained silent and did not confront the comment. We found that confrontation does help women feel better than …

Water Quality Profiles In Two Rolla Ponds: Implications For Nutrient Management, Tylor Cheatham

Undergraduate Research Conference at Missouri S&T

Eutrophication of aquatic ecosystems is a growing global issue due to anthropogenic inputs. One of the main issues associated with eutrophication is fish death. Eutrophic waters can lead to algal blooms, resulting in hypoxic conditions where there is not enough oxygen to support the oxygen-breathing organisms like fish. This can happen in lakes or where rivers meet the ocean. These hypoxic conditions are a concern for lake managers and people who make a living fishing in waters that are now experiencing fish death. One method to target the oxygen levels in lakes is adding aerators. Because of how dynamic ecosystems …

Effects Of Reproduction On Senescence In Local Species, Nathan Smith

Undergraduate Research Conference at Missouri S&T

Understanding the impact of reproduction on the aging process within species populations remains an ongoing challenge in ecological and evolutionary research. In this study, we aim to clarify the relationship between reproduction and senescence using agent-based modeling using wild species data sets. Our objectives include investigating how variations in reproductive rates influence the lifespan and aging trajectories of individuals within populations, as well as identifying potential mechanisms underlying these effects. We will employ agent-based modeling to simulate populations and explore the dynamics of reproduction and senescence using publicly available datasets of local wild species. By manipulating parameters related to reproductive …

Dynamics Of Laboratory Nematode Populations, Erik Bergstrom, Andrea Scharf

Undergraduate Research Conference at Missouri S&T

Understanding how populations fluctuate is important for understanding how evolutionary pressures have shaped the life histories of species, such as reproductive patterns and aging. A laboratory ecosystem consisting of Caenorhabditis elegans and Escherichia coli and wormPOP, a simulation of this laboratory ecosystem, developed by Scharf and colleagues (Scharf et al., 2022) provides an opportunity to better understand population dynamics by tracking individual simulated worms and computing the emergent population dynamics. However, the output data consist of curves with peaks and troughs that are challenging to analyze and that were hypothesized to fit the mathematical definition of chaotic behavior. This project …

Insect Collection Methods At Fort Leonard Wood, Sage Wood, Miranda Ragland, Robin M. Verble

Undergraduate Research Conference at Missouri S&T

This project is part of a larger survey examining the terrestrial insect diversity at Fort Leonard Wood in summers 2021 and 2022. The data analyzed in this poster includes specimens collected during the 2022 collection period. This project attempts to elucidate the most effective method of insect collection at Fort Leonard Wood, as well as any hotspots of insect presence or diversity on the base. Specimens were collected using hand and net collection, Lindgren funnel trapping, leaf litter sampling, and three colors of pan trapping (blue, yellow, white). Specimens were processed in the lab, pinned, sorted, and identified. Preliminary analysis …

Simulating Inter-Species Competition In C. Elegans, Kevin Lai

Undergraduate Research Conference at Missouri S&T

In biological research, understanding the life cycles of Caenorhabditis elegans (C. elegans) is pivotal for insights into developmental biology, genetics, and population dynamics. Our project builds on Worm-Pop, a Python-based multi-agent simulation of Caenorhabditis elegans (C. elegans) , to enhance its capabilities in simulating survival strategies, reproductive success, and genetic drift. The current model simulates a uniform population without inter-agent interactions. I plan to introduce multiple species of worms into the simulation to study competitive dynamics and determine which variants are most successful under various conditions. Pheromones significantly influence C. elegans behavior, affecting mating, foraging, and social interactions. To address …

Cytokinesis And Dbf2, Katharine Gray, Katie Shannon

Undergraduate Research Conference at Missouri S&T

During cell division, the cytoplasm must be divided between the parent and daughter cell. The Mitotic Exit Network (MEN) is a signaling pathway that allows a dividing cell to exit mitosis and complete cytokinesis. Obf2 is a MEN protein kinase in yeast, which provides a simplified model for studying this conserved pathway. By regulating Dbf2s' activity through phosphorylation mutants, its effect on cytokinesis can be studied. Cells were viewed under a microscope, to visualize division errors. Yeast cells that fail to complete cytokinesis will form chains, as the budding daughter cells fail to separate from their parents. Chains of 3 …

Xenobiotic-Lnduced Developmental Neuropathology, Lindsay Schneider

Undergraduate Research Conference at Missouri S&T

As new drugs and pollutants are introduced, a larger subset of people are exposed. The question that arises is whether exposure of a pregnant woman to drugs/pollutants will have negative effects on the neurobiology of her unborn child. We are establishing a screen to test the effects of drugs and pollutants on the developmental neuropathology of the model organism, Caenorhabditis elegans. The roundworm has 302 neurons that are structurally similar to human neurons, and defects in single neurons can be detected by impairment in their neural behaviors such as chemotaxis towards an attractant. Using a chemotaxis assay, we can investigate …

Stream Fungal Biodiversity And Leaf Decomposition, Vale Miller

Undergraduate Research Conference at Missouri S&T

CURRENTLY ONGOING PROJECT We are investigating the possible impact of aquatic fungi biodiversity on leaf litter decomposition and respiration. Four different species of aquatic fungi were placed into flasks with leaf cores (A, B, C, and D), one with no fungi as a control, and one with all four species in equal amounts (ABCD). So far, there seems to be little to no effect on respiration between the species and similar results in the flasks with all four species. Based on this information, biodiversity has yet to show a significant impact in leaf respiration and little to no effect on …