Digital Commons Network^™

Effects Of Lead On Inflammatory Microhemodynamics In Male Wistar Rats, Tanaz Mistry

Seton Hall University Dissertations and Theses (ETDs)

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Chemiluminescent Detection Of Sequential Dna Hybridizations To High- Density, Filter-Arrayed Cdna Libraries: A Subtraction Method For Novel Gene Discovery, D. Guiliano, M. Ganatra, J. Ware, J. Parrot, J. Daub, L. Moran, H. Brennecke, J. M. Foster, T. Supali, M. Blaxter, A. L. Scott, Steven A. Williams, B. E. Slatko

Biological Sciences: Faculty Publications

A chemiluminescent approach for sequential DNA hybridizations to high- density filter arrays of cDNAs, using a biotin-based random priming method followed by a streptavidin/alkaline phosphatase/CDP-Star(TM) detection protocol, is presented. The method has been applied to the Brugia malayi genome project, wherein cDNA libraries, cosmid and bacterial artificial chromosome (BAC) libraries have been gridded at high density onto nylon filters for subsequent analysis by hybridization. Individual probes and pools of rRNA probes, ribosomal protein probes and expressed sequence tag probes show correct specificity and high signal-to-noise ratios even after ten rounds of hybridization, detection, stripping of the probes from the membranes …

Parasitic Helminth Genomics, M. Blaxter, M. Aslett, D. Guiliano, J. Daub, Steven A. Williams, Kunthala Jayaraman, Reda Ramzy, Alan Scott, Tania Supali, Barton Slatko

Biological Sciences: Faculty Publications

The initiation of genome projects on helminths of medical importance promises to yield new drug targets and vaccine candidates in unprecedented numbers. In order to exploit this emerging data it is essential that the user community is aware of the scope and quality of data available, and that the genome projects provide analyses of the raw data to highlight potential genes of interest. Core bioinformatics support for the parasite genome projects has promoted these approaches. In the Brugia genome project, a combination of expressed sequence tag sequencing from multiple cDNA libraries representing the complete filarial nematode lifecycle, and comparative analysis …

The Mayfly Newsletter, Peter M. Grant

The Mayfly Newsletter

The Mayfly Newsletter is the official newsletter of the Permanent Committee of the International Conferences on Ephemeroptera.

Limitation Of Algal Growth By Iron Deficiency In The Australian Subantarctic Region, Peter N. Sedwick, Giacomo R. Ditullio, David A. Hutchins, Philip W. Boyd, F. Brian Griffiths, A. Clive Crossley, Thomas W. Trull, Bernard Queguiner

OES Faculty Publications

In March 1998 we measured iron in the upper water column and conducted iron- and nutrient-enrichment bottle-incubation experiments in the open-ocean Subantarctic region southwest of Tasmania, Australia. In the Subtropical Convergence Zone (∼42°S, 142°E), silicic acid concentrations were low (< 1.5μM) in the upper water column, whereas pronounced vertical gradients in dissolved iron concentration (0.12-0.84 nM) were observed., presumably reflecting the interleaving of Subtropical and Subantarctic waters, and mineral aerosol input. Results of a bottle-incubation experiment performed at this location indicate that phytoplankton growth rates were limited by iron deficiency within the iron-poor layer of the euphotic zone. In the Subantarctic water mass (∼46.8°S, 142°E), low concentrations of dissolved iron (0.05-0.11nM) and silicic acid (< 1μM) were measured throughout the upper water column, and our experimental results indicate that algal growth was limited by iron deficiency. These observations suggest that availability of dissolved iron is a primary factor limiting phytoplankton growth over much of the Subantarctic Southern Ocean in the late summer and autumn.

Francis Harper Papers, Zach S. Henderson Library Special Collections

Finding Aids

This collection consists of the professional and personal papers of Cornell-trained naturalist Dr. Francis Harper. Materials span 1904-1972 and include field notebooks, correspondence, photographs, and audiovisual materials documenting the plant and animal life as well as human ecology of the Okefenokee Swamp area of South Georgia. The photographs within Series 1 show the flora, fauna, and people of the Okefenokee swamp. The photographs were taken by the Harpers while on their trips to the swamp. Photographs are all in black and white and range in size. The Field Journals chronicle his travels in the okefenokee, Canada, and New England. Materials …

Detection Of The Bacteroides Fragilis Group In Water By Nested Polymerase Chain Reaction, James Stuart Ferriss

Honors Capstones

No abstract provided.

Reproduction And Health Of A Beaver (Castor Canadensis) Population In Prince William County, Virginia, Timothy R. Brophy, Carl H. Ernst

Faculty Publications and Presentations

Reproduction and health were studied in a beaver (Castor canadensis) population from Prince William County, Virginia during 1998. Copulating beavers were observed in Quantico Creek at Prince William Forest Park on 22 January. Seven females from Quantico Marine Base were trapped between January and May, sacrificed, and dissected. Those reproductively active weighed over 39 pounds (17.7 kg) and were trapped before 1 March. Mean litter size based on counts of corpora lutea was 4.80 young (3-7); however, litter size based on the number of embryos present was only 2.75 (1-3), comparable to most others reported in the literature. Five of …

Chondrocranial And Oral Morphology Of Leptodactylid Larvae, William Hagood Turner

Master's Theses

I focused my research on Leptodactylidae in order to determine the usefulness of chondrocranial and internal oral characters in systematic and phylogenetic studies of this anuran family. To achieve this goal, I examined the chondrocrania of eight species of the Leptodactylinae: Adenomera marmorata, Edalorhina perezi, Limnomedusa macroglossa, Physalaemus gracilis, P. henselii, P. pustulosus, Pleurodema brachyops, and P. tucumana. Furthermore, I analyzed and described the internal oral anatomy of Physalaeum us gracilis, P. henselii, and Limnomedusa macroglossa and the skeletogenesis of P. gracilis and P. pustulosus. Subsequently, I integrated these data with all available data on the larval chondrocrania and internal …

Biodegradation Of Polycyclic Aromatic Hydrocarbons, İsmai̇l Demi̇r, Zi̇hni̇ Demi̇rbağ

Turkish Journal of Biology

Polycylic aromatic hydrocarbons (PAHs), such as petroleum and petroleum derivatives, are widespread organic pollutants entering the environment, chiefly, through oil spills and incomplete combustion of fossil fuels. Since most PAHs are persist in the environment for a long period of time and bioaccumulate, they cause environmental pollution and effect biological equilibrium dramatically. Biodegradation of some PAHs by microorganisms has been biochemically and genetically investigated. Gene locations for some of these pathways are frequently found in exrachromosomal elements. Determination, isolation and characterization of microorganisms which participate in the bidegradation of PAHs have great significance in the decontamination of the environment in …

Effect Of Dietary Fatty Acids And Starvation On The Fatty Acidcomposition In The Muscle Tissue Of Cyprinion Macrostomusheckel, 1843, M. Ali̇ Akpinar

Turkish Journal of Biology

Variations in the fatty acids compositions of muscle tissue, due to starving and feeding Cyprinion macrostomus (Heckel, 1843) at 35 and 24°C which naturally lives at 35±0.5°C in the Kan-gal Thermal Spring (Sivas) were investigated. No qualitative variation took place in the fatty acid composition of the fish which were fed and star-ved at 35°C. As far as the fish which were fed and starved at 24°C were concerned, it has determi-ned that docosapentaenoic acid (C22:5) and docosahexaenoic acid (C22:6), which are not found in fo-od could be synthesized and linoleic acid (C18:2) percentage was drastically decreased. According to the …

Effects Of Competition On Resource Availability And Growth Of A California Bunchgrass, Andrew R. Dyer, Kevin J. Rice

Faculty Publications

In California, little is known about the sensitivity of native bunchgrasses to competition or to changes in resource availability. We investigated the effect of nonnative annual vegetation on resource availability and growth of a native bunchgrass, Nassella pulchra, in a pair of factorial field experiments that incorporated effects of both interspecific and intraspecific competition as well as variation in soil depth. Plots of differing target densities and neighborhoods were used to assess changes in aboveground (light) and belowground (water) resource availability over multiple seasons in two sites with differing soil depth. N. pulchra grown without interspecific competitors grew larger …

Investigation Of The Glucose-Induced Inactivation Of Maltose Permease In Saccharomyces, Igor L. Medintz

Dissertations, Theses, and Capstone Projects

The addition of glucose to maltose fermenting Saccharomyces cerevisiae cells results in the rapid loss of maltose transport activity. This results both from the repression of the maltose permease gene transcription and from the post-translational inactivation of maltose permease through a process termed glucose-induced inactivation of maltose permease. The inactivation consists of two separable processes, a rapid inhibition of maltose transport activity and by a slower degradation of maltose permease protein. Degradation is dependent on endocytosis, vesicular sorting and vacuolar proteolysis, and is independent of the proteasome. Furthermore, maltose permease exists in differentially phosphorylated forms.

Ubiquitin and the ubiquitin-conjugating system …

Microbial Colonization Of The Cloacae Of Nestling Tree Swallows, Tamara K. Mills, Michael P. Lombardo, Patrick A. Thorpe

Peer Reviewed Publications

Microbes have the potential to be important selective forces in many aspects of avian biology. Microbes can affect fitness as a result of either their pathogenic or beneficial effects on host health. Little is known about the chronology of microbial colonization of nestlings or the effects of microbes on fledgling condition. We set out to (1) characterize the time course of microbial colonization of the cloacae of nestling Tree Swallows (Tachycineta bicolor), (2) examine the relationship between cloacal microbes and fledgling condition, and (3) determine if nest mates had similar assemblages of cloacal microbes. We repeatedly measured nestlings …

Distribution Of Desmin And Vimentin In The Rat Endometrium Duringearly Pregnancy, Emi̇n Türkay Korgun, Mevlüt Asar, Necdet Demi̇r, Ramazan Demi̇r

Turkish Journal of Biology

The aim of these studies is to analyze, by immunohistochemical methods, the emergence and distribution of vimentin and desmin in stromal cells of rat endometrium in early pregnancy and determine their role in decidualization and implantation biology. For this purpose totally 1-8 days pregnant 64 female and 32 male rats were used. On first day of pregnancy vimentin and third day of pregnancy desmin were staining respectively in the endometrial stromal tissue of rat. Although vimentin was seen during the early pregnancy, following the day 5 of pregnancy when the implantation begins interestingly desmin was only located in the antimezometrial …

Foreward, Virginia Journal Of Science

Virginia Journal of Science

Foreward to the Winter 1999 issue of Virginia Journal of Science, discussing Pfiesteria Piscicida, written by Harold G. Marshall.

Extraction And Characterisation Of Essential Oils From Waste Eucalyptus Foliage, Hugh O'Reilly

Theses

Extraction procedures such as steam distillation, solvent and soxhlet extraction have been investigated to determine the most effective method of extracting essential oil from the foliage of a number of species of Eucalyptus cultivated in Co. Kerry. Steam distillation has been found to be the most effective method of extraction. The steam distillation extraction method has been thoroughly studied in order to determine the factors affecting yield and composition of the distillate. Alteration of the distillation time has been found to produce oil high in 1,8-cineole content, a requirement of medicinal quality oil. The choice of individual distillation still used …

Spatial Learning Of An Escape Task By Young Corn Snakes, Elaphe Guttata Guttata, David A. Holtzman, Terrence W. Haris, Guillermo Aranguren, Elizabeth Bostock

Biology Faculty Publications and Presentations

Spatial learning is critical to most animals for many behaviours necessary to survival. In vertebrates, most studies on spatial learning and memory have been conducted in mammalian and avian species with few studies on reptiles. We examined spatial learning in the corn snake, Elaphe guttata guttata by training 17 young snakes to find the one open shelter in an eight-hole arena, where the entrance was not visible from the arena surface. Over a 16-trial, 4-day training period, snakes showed (1) a significant decrease in the mean latency to the goal, (2) a significant decrease in the mean total distance travelled, …

Monitoring Results For Pfiesteria Piscidida And Pfiesteria-Like Organisms From Virginia Waters In 1998, Harold G. Marshall, David W. Seaborn, Jennifer Wolny

Biological Sciences Faculty Publications

Results of an extensive 1998 monitoring program for the presence of Pfiesteria-like organisms (PLO) in Virginia estuaries indicate these dinoflagellates are widely distributed in both the water column, and as cysts in the sediment, however Pfiesteria piscicida was not detected at this time. The highest concentrations of PLO were in estuaries along the Virginia shore line of the Potomac River, and in western Chesapeake Bay estuaries from the Little Wicomico River to the Rappahannock River. The most common PLO included Cryptoperidiniopsis sp. and Gymnodinium galatheanum. The lowest PLO concentrations were at ocean side locations. PLO were also …

Role Of Iron, Light, And Silicate In Controlling Algal Biomass In Subantarctic Waters Se Of New Zealand, Robert Michael Mckay, Philip Boyd, Julie Laroche, Mark Gall, Russell Frew

Biological Sciences Faculty Publications

Phytoplankton processes in subantarctic (SA) waters southeast of New Zealand were studied during austral autumn and spring 1997. Chlorophyll a (0.2–0.3 μg L⁻¹) and primary production (350–650 mg C m⁻² d⁻¹) were dominated by cells μm (cyanobacteria) in both seasons. The photochemical efficiency of photosystem II (F_υ/F_m) of cells was low (0.3), indicating physiological stress. Dissolved Fe (DFe) levels in surface waters were subnanomolar, and the molecular marker flavodoxin indicated that cells were iron stressed. In contrast, Subtropical Convergence (STC) and subtropical waters had higher algal biomass/production levels, particularly …

The Effect Of Beach Nourishment On Loggerhead (Caretta Caretta) Nesting And Reproductive Success At Sebastian Inlet, Florida, Richard Michael Herren

Retrospective Theses and Dissertations

Beach nourishment has become common in Florida and it occurs on beaches that are major loggerhead (Caretta caretta) nesting grounds. Despite efforts to use beach-quality sand, nourishment sand may be different in grain size, moisture content, shear resistance and temperature when compared to native sand. Two main aspects of loggerhead nesting may be affected by nourishment. First, nourishment may reduce nesting success [(female nesting emergences/ female total emergences) X 100] due to physical barriers (i.e., scarps or steep cliffs) that can impede gravid females. Second, nourishment may reduce reproductive success {i.e., hatching success) by altering the nestsand environment. The objective …

Effects Of Sludge Application On Prairie Establishment, Brenda Joan Durbahn

Dissertations and Theses @ UNI

Native prairie in Iowa has all but disappeared since the development of agricultural land by European settlers over the past 165 years. Reconstructing prairie is one way to replace some of the acreage that was lost. A byproduct of settling an area is the generation of garbage and other wastes. Currently there is a surplus of sludge, the waste product of waste water treatment facilities. This material is usually disposed of in landfills, used on agricultural land or reclamation projects.

A small area of prairie was reconstructed on the top of a closed portion of the Black Hawk County Solid …

Vicial.'Nin Üreme Biyolojisi Ii. V. Galileaeplitm. &Zoh.'De Anter Çeperi, Mikrosporogenez, Polenmitozu Ve Erkek Gametofitin Gelişiminin Sitolojikve Sitoembriyolojik Yönden Incelenmesi, Feruzan Dane, Çi̇ler Meri̇ç

Turkish Journal of Biology

The cells at the tip of the roots of germinated seeds of Vicia galileae were caryologycally examined and the chromosome number was found to be 2n=14. The strach grains were observed in the epidermis, endothecium and middle layer cells in the early development phases of the anther walls. Normal mitotic divisions were observed in the glandular type tapetum. Also it was observed that meiotic divisions in most of the microspore mother cells were regular and irregularities were only 1- 2%. Polarization which occurred normally in pollen mitosis and the divison stages were clearly observed. It was noted that pollens were …

The Effect Of Zeaxanthin And Tank Color On Pigmentation Andgrowth Of Goldish ( Carassius Auratus), Mahmut Yanar, Nazmi̇ Tekeli̇oğlu

Turkish Journal of Biology

In this study, the effect of zeaxanthin and tank color on pigmentation and growth of godfish ( Carassius auratus ) was investigated. Total carotenoid deposition in the fish skin was determined spectrophotometrically. At the end of 60 days of experimental period, total carotenoid accumulation in the skin of fish feeding the same food containing 75 mg/kg zeaxanthin and grown in green, blue, red, white and yellow tanks were 34.41±0.56, 32.90±0.42, 28.60±0.74, 28.58±0.52, and 26.96±0.70 mg/kg, respectively. Pigmentation in green and blue tanks were higher than the other tanks (p

Substrate And Enzyme Profile Of Fast And Slow Skeletal Muscle Fibers In Rhesus Monkeys, Varvara P. Grichko, Gwen J. Gettelman, Jeffrey Widrick, Robert H. Fitts

Biological Sciences Faculty Research and Publications

Results from the Russian Cosmos program suggest that the rhesus monkey is an excellent model for studying weightlessness-induced changes in muscle function. Consequently, the purpose of this investigation was to establish the resting levels of selected substrate and enzymes in individual slow- and fast-twitch muscle fibers of the rhesus monkey. A second objective was to determine the effect of an 18-day sit in the Spacelab experiment-support primate facility [Experimental System for the Orbiting Primate (ESOP)]. Muscle biopsies of the soleus and medial gastrocnemius muscles were obtained 1 mo before and immediately after an 18-day ESOP sit. The biopsies were freeze-dried, …

Detection Of Ehrlichia Chaffeensis In Ticks Of The Georgia Coast And Barrier Islands Using Polymerase Chain Reaction, John Edward Whitlock

College of Graduate Studies: Theses & Dissertations (1964–2006)

Human monocytic ehrlichiosis (HME) is an emerging, zoonosis transmitted to mammals by ixodid ticks. Prevalence of infected ticks and distribution of infection foci indicate relative risk of human exposure to ehrlichiosis and may be influenced by factors such as geographic isolation and human disturbance. To test this, individual and pooled lone star ticks, Amblyomma americanum (L.), collected from 3 populations from the coast and barrier islands of Georgia were screened for Ehrlichia chaffeensis Anderson, Dawson, Jone, and Wilson, the agent of HME. A species-specific, nested polymerase chain reaction (PGR) assay was used to amplify a 572 base pair fragment of …

Field Sampling And Necropsy Examination Of Fish, A. S. Kane, A. Baya, R. Reimschuessel, K. M. St. Pé, C. A. Poukish, C. P. Driscoll

Virginia Journal of Science

This paper presents an overview of observational and fish sampling tech­niques for investigating fish lesions, morbidity and mortality. These sam­pling techniques and investigations are much like detective work and require attention to detail, common sense, technical proficiency and experience. To solve the mystery of a fish kill, the investigator must use available evidence and clues to piece together a series of events that often have long since passed. The cause of these field events may be chemical, biological or physical; more often, it is some combination of these. An initial categorization approach may be used to reduce the great number …

Pcr And Fish Detection Extends The Range Of Pfiesteria Piscicida In Estuarine Waters, P. A. Rublee, J. Kempton, E. Schaefer, J.M. Burkholder, H. B. Glasgow Jr., David Oldach

Virginia Journal of Science

PCR and fluorescent in situ hybridization probes were used to assay for the presence of the dinoflagellate Pfiesteria piscicida in 170 estuarine water samples collected from New York to northern Florida. 20% of samples tested positive for the presence of P. piscicida, including sites where fish kills due to Pfiesteria have occurred and sites where there was no historical evidence of such events. The results extend the known range of P. piscicida northward to Long Island, New York. The results also suggest that P. piscicida is common, and normally benign, inhabitatant of estuarine waters of the eastern US.

Virginia's Pfiesteria Monitoring Program: Water Quality, Roger K. Everton, Wick T. Harlan, Jonathan W. Priest, Mark S. Alling

Virginia Journal of Science

During the summer and early fall of 1998, 34 estuarine stations in Virginia were sampled for Pfiesteria like organisms (PLOs) and associated water quality conditions. Stations were sampled either bimonthly (20 stations) or monthly (14 stations) from June to October 1998. At each station, a set of live and Lugol' s preserved samples were collected for presumptive counts of PLOs and water quality conditions were determined. Water quality parame­ters measured included standard field parameters, nutrients (total, dissolved, and particulate), chlorophyll a, and conventional water quality indicators. Although PLOs were relatively low in 1998 as compared to 1997, presump­tive PLO counts …

A Cohort Study To Determine The Epidemiology Of Estuary-Associated Syndrome, Elizabeth Turf, Lily Ingsrisawang, Megan Turf, J.D. Ball, Michael Stutts, John Taylor, Suzanne Jenkins

Virginia Journal of Science

From the Introduction:

Estuary-Associated Syndrome (EAS) is the name given to a potential illness characterized primarily by changes in an individual's cognitive abilities, including acute onset of memory loss or the sudden inability to solve simple problems. Other possible signs of illness include respiratory symptoms, skin rash, or gastrointestinal distress. This illness appears to arise following exposure to toxin produced by Pfiesteria piscicida, or other toxic dinoflagellates, that resides in estuary waters.

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In order to learn more about this possible syndrome and to determine if a causal relationship exists between association to waters containing Pfiesteria or other toxic …