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A Study Of The Levels Of Vitamins A, E And C And Selenium In Rhubarb(Rheum Ribes L.), Ömer Munzuroğlu, Fi̇kret Karataş, Nazmi̇ Gür Jan 2000

A Study Of The Levels Of Vitamins A, E And C And Selenium In Rhubarb(Rheum Ribes L.), Ömer Munzuroğlu, Fi̇kret Karataş, Nazmi̇ Gür

Turkish Journal of Biology

The levels of vitamins A, E and C and selenium in young shoots of Rheum ribes L. collec-ted at various altitudes from Elazığ and Tunceli Basins were examined experimentally. The vitamin le-vels and the amount of selenium were determined by HPLC and fluorometers. It was determined that the levels of A, E and C vitamins and selenium in the young shoots of Rheum ribes L. were in the ranges of 0.255 ± 0.019 - 0.363 ± 0.015 µg.gr -1 , 0.614 ± 0.014 - 0.765 ± 0.021 µg.gr -1 , 197.6 ± 10.47 - 282.3 ± 17.46 µg .gr -1 …

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Production Of Gibberellic Acid And Cytokinin By Phanerochaete Chrysosporiumme446 Immobilized On Polyurethane Foam, Serpi̇l Ünyayar Jan 2000

Production Of Gibberellic Acid And Cytokinin By Phanerochaete Chrysosporiumme446 Immobilized On Polyurethane Foam, Serpi̇l Ünyayar

Turkish Journal of Biology

The fungus Phanerochaete chrysosporium ME446 was entrapped on polyurethane foam to synthesize gibberellic acid (GA 3 equivalent) and Cytokinin (zeatin equivalent). Maximum gibberellic acid was defined on the 6 th day (44.43 µg/mL and 61.22 µg/mL, respectively) in both free and immobilized cells. Maximum cytokinin was defined on the 12 th day (9.61µg/mL and 16.33 µg/mL, respectively) in both free and immobilized cells. An immobilized system of gibberellic acid and cytokinin production resulted in comparatively higher yield than free fungal mycelium.

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Eukaryotic Transcriptional Regulatory Proteins, Kemal Büyükgüzel Jan 2000

Eukaryotic Transcriptional Regulatory Proteins, Kemal Büyükgüzel

Turkish Journal of Biology

Transcriptional regulation is of considerable importance in the expression of eukaryotic genes, and a considerable degree of regulation is controlled at the level of mRNA synthesis. As in prokaryotic organisms, transcriptional control in eukaryotes results from an interplay between regulatory DNA sequences and site-specific DNA-binding proteins. Transcription of eukaryotic genes is influenced by various regulatory elements, termed promoters, enhancers and silencers. These regulatory elements are composed of discrete DNA sequences, which constitute binding sites for sequence-specific DNA-binding proteins. These proteins bind DNA by some common structural motifs. The motifs that are utilized by eukaryotic regulatory proteins are helix-turn-helix, zinc finger …

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Some Kinetic Properties Of Hepatic Lipase Of Oncorhynchus Mykisswalbaum, 1792, Kubi̇lay Meti̇n, Mehmet Ali̇ Akpinar Jan 2000

Some Kinetic Properties Of Hepatic Lipase Of Oncorhynchus Mykisswalbaum, 1792, Kubi̇lay Meti̇n, Mehmet Ali̇ Akpinar

Turkish Journal of Biology

Some kinetic properties of hepatic lipase of Oncorhynchus mykisswere investigated. Hepatic triacylglycerol lipase was found to be located in the cytosolic fraction of the cell. Optimal activity of hepatic lipase occurred at pH 8.00 and 32 °C. When pNPA was used as a substrate, Vmax and Km values of hepatic lipase were estimated as 0.40 U/mg protein and 0.12 mM pNPA respectively. A stimulating effect of monovalent (NaCl and KCl) and divalent (CaCl 2 and MgCl 2 ) metal ions on hepatic lipase activity was determined. The bile salts, Na-taurodeoxycholate and Na-deoxycholate, were determined to inhibit hepatic lipase activities. A …

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Immunohistochemical Distribution Of Transforming Growth Factor Alpha,Laminin, Fibronectin And Desmin In Rat Ovary, Gökhan Akkoyunlu, İsmai̇l Üstünel, Ramazan Demi̇r Jan 2000

Immunohistochemical Distribution Of Transforming Growth Factor Alpha,Laminin, Fibronectin And Desmin In Rat Ovary, Gökhan Akkoyunlu, İsmai̇l Üstünel, Ramazan Demi̇r

Turkish Journal of Biology

Since the pure synthesis of transforming growth facto alpha (TGF\alpha) many attempts have been made in order to determine its distribution in various tissues. As a result of the complexitiy of the cyclic periods throughout experimental models, it is not easy to determine the distribution of TGF\alpha in the system. Especially the pre-and through-gestational pattern of TGF\alpha in rat ovary has not been reported fully. Furthermore, the shortage of literature about intracellular desmin and laminin, fibronectin of different intermediate filaments whether they have any interaction with TGF\alpha through this period, makes the subject worth discussing. Immunocytochemical techniques were utilized in …

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Insecticide Resistanca In Maleria Vector An. Sacharovi, Ümi̇t Lüleyap, Hali̇l Kasap Jan 2000

Insecticide Resistanca In Maleria Vector An. Sacharovi, Ümi̇t Lüleyap, Hali̇l Kasap

Turkish Journal of Biology

Susceptibility tests have been successfully used for many years to determine insecticide resistance raised in pest and vector insects, including An. sacharovi,the primary human malaria vector in Turkey. As this method does not provide sufficient information about physiological resistance, an alternative method of enzyme tests based on biochemical and genetic evaluations has been developed. In this study, both methods were used for comparison. First of all, susceptibility to insecticides was tested in adult females of An. sacharovi collected in different seasons from Tabaklar and Herekli Villages, where insecticides are used intensively for both agricultural and vector control purposes, and from …

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The Effects Of Different Carbon Sources And C/N Ratio On Microbialdenitrification, Gülten Ökmen Kurucuoğlu, Ömer Faruk Algur Jan 2000

The Effects Of Different Carbon Sources And C/N Ratio On Microbialdenitrification, Gülten Ökmen Kurucuoğlu, Ömer Faruk Algur

Turkish Journal of Biology

The effects of different carbon sources (ethanol, methanol and acetic acid) and C/N ratios (0,5; 1; 1,5; 2; 2,5) on the microbial denitrification in batch culture were investigated. For this purpose Paracoccus denitrificans and Pseudomonas stutzeri were used as denitrifiers. For all experiments, the initial NO 3 concentrations were constant at 100 mg/l. During the incubation period of 66 hours, nitrate, nitrite and microorganism concentrations were determined spectrophotometrically at certain intervals. When ethanol was used as a carbon source, the highest NO 3 reductions for P. denitrificans and P. stutzeriwere found to be 40% (C/N = 1) and 32% (C/N …

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Fungal Contamination And The Incidence Of Ochratoxin-A In Some Cerealsand Products, Nural Kargözlü, Mehmet Karapinar Jan 2000

Fungal Contamination And The Incidence Of Ochratoxin-A In Some Cerealsand Products, Nural Kargözlü, Mehmet Karapinar

Turkish Journal of Biology

One hundred samples (wheat, corn, corn flour, oat, oat flour, muesli) were analysed for ochratoxin-A and fungal contamination. Ochratoxin-A was detected in four samples at the level of 0.27-9.84 ppb in one wheat, two corn and one oat flour samples. Total mould and yeast counts were detected to be 20-

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The Effect Of Soy Flour On Alcohol And Cell Concentration In Alcoholfermentation Of Molasses, Tuncay Ari, Sedat Dönmez Jan 2000

The Effect Of Soy Flour On Alcohol And Cell Concentration In Alcoholfermentation Of Molasses, Tuncay Ari, Sedat Dönmez

Turkish Journal of Biology

The effects of soy flour on alcohol fermentation of molasses by different yeast strains were investigated. Six strains of Saccharomyces cerevisiae and one Saccharomyces bayanus were used. It was found that soy flour affected fermentation time, alcohol concentration and total cell count in all strains. But the degree of this effect was different among strains. The maximum ethanol concentration were 63.9 g/l in Saccharomyces cerevisiae N3 strains. The maximum theoritical yield, however was 91.6% in Saccharomyces cerevisiae W4 strains. Compared with the control, the largest increment in ethanol concentration was in Saccharomyces bayanus 1A as 12.2%. Furthermore, addition of soy …

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The Effect Of Cadmium Nitrate On Some Developmental Properties Ofdrosophila Melanogaster, Lütfi̇ye Gelegen, Eli̇f Yeşi̇lada Jan 2000

The Effect Of Cadmium Nitrate On Some Developmental Properties Ofdrosophila Melanogaster, Lütfi̇ye Gelegen, Eli̇f Yeşi̇lada

Turkish Journal of Biology

The effects of cadmium nitrate on the fecundity, larval survival and sex ratio of D. melanogaster were tested. According to our findings, the effects of cadmium nitrate can be summarized as follows: it appeared that when used as a feeding substance during the developmental period, cadmium nitrate did not have any effect on fecundity. On the other hand, if cadmium nitrate was given throughout adult life it caused a proportional decrease in the daily mean with increasing concentration. Furthermore, the larval survival ratio decreased due to the concentration of cadmium nitrate. Also cadmium nitrate application appeared to have no effect …

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Obtaining Biosurfactant From Olive Oil Mill Effluent (Oome) Usingpseudomonas Sp., Uğur Sidal, Nazi̇f Kolankaya, Cengi̇z Kurtonur Jan 2000

Obtaining Biosurfactant From Olive Oil Mill Effluent (Oome) Usingpseudomonas Sp., Uğur Sidal, Nazi̇f Kolankaya, Cengi̇z Kurtonur

Turkish Journal of Biology

Olive oil mill effluent (OOME) is one of the major pollutants produced by the agricultural industry in Mediterranean countries. The olive oil mills in these countries generate about 2-3 million tonnes of effluent annually. OOME is a black liquor containing a water-soluble fraction of ripe olives and water that is used in the process of olive oil extraction. This waste has a high mineral content and a chemical oxygen demand of 25.000-100.000 mg/l. depending on the olive oil extraction process used. This study presents a process for biosurfactant as rhamnolipid production by Pseudomonas sp. from olive oil mill effluent (OMME). …

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Effects Of Exogenous Spermine On Seed Germination And Early Growthof Sunflower Seeds Under Salinity Stress, Fatma Mutlu, Suna Boncuk Jan 2000

Effects Of Exogenous Spermine On Seed Germination And Early Growthof Sunflower Seeds Under Salinity Stress, Fatma Mutlu, Suna Boncuk

Turkish Journal of Biology

The effects of various concentrations (0.01, 1, 2mM) of spermine (Spm) on germination and some early growth parameters of sunflower ( Helianthus annuus L. cv. Santafe) seeds were investigated in media containing different concentrations of NaCl (50, 100, 200mM). Regardless of its concentrations, application of Spm under non-saline conditions remained ineffective on germination percentage as well as on the early growth parameters studied (length of radicle, fresh and dry weights). The salt, depending on its concentration, inhibited or delayed the germination to a great extent. The growth parameters studied in the early growth period were also adversely affected by salt …

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Study Of Adventitious Shoot Formation In Leaves Of Grape (Vitis Spp.), Ni̇lgün Göktürk Baydar Jan 2000

Study Of Adventitious Shoot Formation In Leaves Of Grape (Vitis Spp.), Ni̇lgün Göktürk Baydar

Turkish Journal of Biology

The effects of genotype, source of explant and nutrient medium on adventitious shoot formation were examined in the leaves of 5 grape cultivars (Atasarisi, Çavus, Kalecik Karasi, Sultani çekirdeksiz and Yapincak), 2 rootstocks (Kober 5BB ( V. berlandieri x V. riparia) and 41 B M.G. (Chasselas x V. berlandieri)) by organogenesis. In this study, petioles and laminae isolated from in vitro shoots were used as the sources of explant. The highest rates of adventitious shoot formation were obtained from petiole explants. Leaf explants were cultured on MS- and NN- based regeneration media containing different concentrations of BAP and NAA. It …

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Phage Sensitivities Of Lactococci Isolated From Raw Milk And Whey, Pinar Şanlibaba, Mustafa Akçeli̇k Jan 2000

Phage Sensitivities Of Lactococci Isolated From Raw Milk And Whey, Pinar Şanlibaba, Mustafa Akçeli̇k

Turkish Journal of Biology

In this study, lactococcal strains isolated from raw milk and whey samples of different types of cheese produced without using industrial starter cultures in different regions of Turkey were tested for sensitivity to 22 lactic phages. In addition to the strain isolation materials, virulent lactic phages were also isolated from samples obtained from dairy plants using industrial starter cultures. 18 L lactis subsp. lactis strains out of 73 lactococcal strains were found to be resistant to all the phages. It was determined that the total phage resistance levels of the other 55 strains were between 18.18 and 90.90%.

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Karyotype Analysis In Chalcalburnus Mossulensis (Heckel, 1843), Süleyman Gül, Ahmet Çolak, İlhan Sezgi̇n Jan 2000

Karyotype Analysis In Chalcalburnus Mossulensis (Heckel, 1843), Süleyman Gül, Ahmet Çolak, İlhan Sezgi̇n

Turkish Journal of Biology

Karyotype analysis was performed in Chalcalburnus mossulensis (Heckel, 1843) belonging to the carp family by investigating the number and structures of their chromosomes. The fish used in this study were caught with fishing nets from Kizilirmak river and taken to the laboratory. They received an abdominal injection of 0.6 mg colchicine (Colchicum Dispert) dissolved in 1 ml of distilled water per 1 g body weight. As a result of the C-metaphase investigation, it was determined that C. mossulensis had 2n=48 chromosomes. Their karyotypes were determined to be composed of 6 metacentric, 10 submetacentric, and 8 acrocentric chromosome pairs. We were …

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Boron Accumulation In Carp Tissues ( Cyprinus Carpio L., 1758) In Damlakes Çötören And Kunduzlar (Kırka-Eskişehir), Şaki̇r Özkurt Jan 2000

Boron Accumulation In Carp Tissues ( Cyprinus Carpio L., 1758) In Damlakes Çötören And Kunduzlar (Kırka-Eskişehir), Şaki̇r Özkurt

Turkish Journal of Biology

Samples of water, bottom clay, plankton and fish were collected over a period of one year. The boron contents of the samples taken were determined in the laboratory. The differences and relationships in the obtained data were identified using various statistical methods. Boron gradually accumulates in the plankton, bottom clay and water of Çatören and Kunduzar. Boron collects in the tissues of the fish Cyprinus carpio in both lakes, in the musce, brain and liver in Çatören, and in the musce, liver and brain in Kunduzlar. Boron pollution is more serious in Çatören dam than in Kunduzlar. It was observed …

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The Effects Of Heavy Metals On The Pollen Germination And Pollen Tubegrowth Of Apples (Malus Sylvestris Miller Cv. Golden), Ömer Munzuroğlu, Nazmi̇ Gür Jan 2000

The Effects Of Heavy Metals On The Pollen Germination And Pollen Tubegrowth Of Apples (Malus Sylvestris Miller Cv. Golden), Ömer Munzuroğlu, Nazmi̇ Gür

Turkish Journal of Biology

The effect of heavy metals, namely mercury (Hg^{+2}), zinc (Zn^{+2}), nickel (Ni^{+2}) and cobalt (Co^{+2}), which were applied in the form of chloride salt, and that of lead (Pb^{+2}) and cadmium (Cd^{+2}) applied in the form of nitrate salt, on the pollen germination and tube growth were investigated on apples ( Malus sylvestris cv. Golden). According to the results obtained, these heavy metals led to a significant decrease in the pollen germination and tube growth. It was found that there was a parallelism between the concentration of heavy metal salt, and pollen germination and tube growth. Among the heavy metals …

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Prevention Of Hydrogen Peroxide And Cisplatininduced Apoptosis By Intracellular Catalaseoverexpression, H. Barbaros Oral, Andrew J.T. George, Dorian O. Haskard Jan 2000

Prevention Of Hydrogen Peroxide And Cisplatininduced Apoptosis By Intracellular Catalaseoverexpression, H. Barbaros Oral, Andrew J.T. George, Dorian O. Haskard

Turkish Journal of Biology

Apoptosis induced by hydrogen peroxide (H_2O_2 ) was investigated in Chinese hamster ovarian (CHO) cells, and compared to apoptosis induced by cisplatin (25 µM), a well-established apoptosis-inducing agent. In preliminary experiments, CHO cells were treated with low concentrations of H_2O_2 (250-500 µM) continuously or for 3 hours, followed by washing and further incubation in complete growth medium for 1-10 days. Fluorescence microscopy of acridine orange-stained cells indicated morphological changes of apoptosis. Similar changes were observed in cisplatin-treated cells. DNA-laddering also revealed that DNA fragmentation occurred in both adherent and non-adherent cells by day 2. To prevent H_2O_2 -mediated apoptosis, the …

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Production Of Monoclonal Antibodies Specific Forprogesterone, Estradiole By Simultaneous Injectionof Different Steroids, Fatma Şahi̇ngöz Yücel, Beyazit Çirakoğlu Jan 2000

Production Of Monoclonal Antibodies Specific Forprogesterone, Estradiole By Simultaneous Injectionof Different Steroids, Fatma Şahi̇ngöz Yücel, Beyazit Çirakoğlu

Turkish Journal of Biology

We report here the development of hybrid cells producing monoclonal antibodies specific for two different steroid hormones with mixed immunization using hybridoma technology. BALB/c mice were immunized with a mixture of three steroid antigens: progesterone, estradiole and testosterone linked to bovine serum albumine. These mice were used for fusion. In the two fusion experiments, ELISA tests showed that among 645 wells only 2 hybrids reacted with progesterone (MAM 3C2, MAM 3E3) and one of them reacted specifically with estradiol (MAM 7H7). Three types of monoclonal antibodies were detected: IgG1, IgG2a and IgG2b and the affinities of these antibodies were found …

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Transmission Of The Embryonic Stem Cell (Esc)Genome To Offspring And Sexual Development Inchimeric Mice From A Male Esc Line, Sezen Arat Jan 2000

Transmission Of The Embryonic Stem Cell (Esc)Genome To Offspring And Sexual Development Inchimeric Mice From A Male Esc Line, Sezen Arat

Turkish Journal of Biology

This study was performed to determine the capability of embryonic stem (ES) cells at late passage number to form functional germ cells and to orientate the sexual development of chimeric mice produced from ES cell injection into embryos. When an R1 ES cell line was routinely cultured on mitotically inactivated feeder cells in culture medium supplemented with leukemia inhibitory factor (LIF), the colonies maintained an undifferentiated ideal ES cell morphology. When the ES cells were injected into 45 eight-cell embryos, 38 embryos (84%) combined with ES cells. Twenty-eight percent of the embryos transferred to recipients developed to term. Thirty percent …

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Effects Of Ph On The Genesis Of Membrane Potentialchanges At Fertilization In The Egg Of The Frog Ranacameranoi, Şeref Erdoğan, Gülay Loğoğlu, Kerem Tuncay Özgünen, Tuncay Özgünen Jan 2000

Effects Of Ph On The Genesis Of Membrane Potentialchanges At Fertilization In The Egg Of The Frog Ranacameranoi, Şeref Erdoğan, Gülay Loğoğlu, Kerem Tuncay Özgünen, Tuncay Özgünen

Turkish Journal of Biology

The apparent change in the egg membrane potential at fertilization gives rise to fertilization potential (FP) . FP is the initial, transient electrical block to polyspermy as shown in most species especially those exhibiting external fertilization, and it protects the egg from a second sperm entry until the permanent, mechanical block is set up. Polyspermy is lethal in most species, and for a successful fertilization resting membrane potential (RMP) and FP of the egg have to be held in optimum ranges. Shifts in environmental pH may interfere with a successful fertilization by affecting these bioelectrical potentials. In the present study, …

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Characterization Of Endoxylanase Activity Fromthermomonospora Fusca Bd25, Müni̇r Tuncer Jan 2000

Characterization Of Endoxylanase Activity Fromthermomonospora Fusca Bd25, Müni̇r Tuncer

Turkish Journal of Biology

Thermomonospora fuscaBD25 produces relatively high levels of activity of endoxylanases. Only oat spelt xylan, RBB-xylan and birchwood xylan reacted with endoxylanases with the maximum specific activity of 47.65 U mg -1 protein on birchwood xylan. The endoxylanase remained stable up to 70 °C. At 60 °C endoxylanase activity showed stability (92 %) for 16 h, however it showed a half-life of 8 h at 70 °C and 30 min at 80 °C in the absence of substrate at pH 8.0. The relative endoxylanase activity in the pH ranges of 6.5 to 9.5 remained between 68 % and 72 % of …

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Characterization Of \Beta-Xylosidase And\Alpha-L-Arabinofuranosidase Activities Fromthermomonospora Fusca Bd25, Müni̇r Tuncer Jan 2000

Characterization Of \Beta-Xylosidase And\Alpha-L-Arabinofuranosidase Activities Fromthermomonospora Fusca Bd25, Müni̇r Tuncer

Turkish Journal of Biology

Thermomonospora fuscaBD25 produces relatively high levels of activity of \beta-xylosidase and \alpha-L-arabinofuranosidase. The aim of the work described in this study was to characterize some properties of \beta-xylosidase and \alpha-L-arabinofuranosidase produced by T. fusca BD25 when growing on oat spelt xylan as the main carbon and energy sources. The substrates p-NPX and p-NPA reacted with \beta-xylosidase and \alpha-L-arabinofuranosidase with specific activities of 4.01 U mg -1 protein and 0.35 U mg -1 protein, respectively. The \beta-xylosidase remained stable at up to 65 °C, but \alpha-L-arabinofuranosidase lost 10 % of its maximum activity at 55 °C. \beta-xylosidase and \alpha-L-arabinofuranosidase activities remained …

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Production Of Auxin And Abscisic Acid Byphanerochaete Chrysosporiumme446 Immobilizedon Polyurethane Foam, Serpi̇l Ünyayar, Eli̇f Ünal, Ali̇ Ünyayar Jan 2000

Production Of Auxin And Abscisic Acid Byphanerochaete Chrysosporiumme446 Immobilizedon Polyurethane Foam, Serpi̇l Ünyayar, Eli̇f Ünal, Ali̇ Ünyayar

Turkish Journal of Biology

The fungus Phanerochaete chrysosporiumME446 was entrapped on polyurethane foam to synthesize auxin and abscisic acid (ABA). The maximum amounts of auxin produced on the 18 th day in both free and immobilized cells were 55.91mg/mL and 76.07mg/mL, respectively. Maximum ABA concentrations on the 12 th day in both free and immobilized cells were 6.87 mg/mL and 10.69 mg/mL, respectively. An immobilized system of auxin and ABA production resulted in a comparatively higher yield than free fungal mycelium.

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Temporal And Spatial Determination Of Germinbiosynthesis In Wheat Tissues, Mahmut Çalişkan, Andrew C. Cuming Jan 2000

Temporal And Spatial Determination Of Germinbiosynthesis In Wheat Tissues, Mahmut Çalişkan, Andrew C. Cuming

Turkish Journal of Biology

In the current study, the biosynthesis of germin was determined in the organs of wheat embryos during germination. The temporal and spatial determination of germin protein was carried out by employing an anti-germin sera in gel-blot analysis. Germin was detected in the coleoptile, roots and vascular transition region of and 6-day-old germinated wheat seedlings. It was shown that the leaf tissue acquires germin accumulation only at the late stage of germination. The pellet fraction of protein homogenate obtained from embryos at different ages revealed higher germin accumulation than the supernatant fraction from the same sources.

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Electrophoretic Analysis Of Candida Albicans Isolatescollected From Turkish Hospitals By Native-Polyacrylamidegel Electrophoresis (N-Page), Özlem Osmanağaoğlu, Sefa Can Saçilik, Ufuk Gündüz, Nurten Altinlar, Cumhur Çökmüş Jan 2000

Electrophoretic Analysis Of Candida Albicans Isolatescollected From Turkish Hospitals By Native-Polyacrylamidegel Electrophoresis (N-Page), Özlem Osmanağaoğlu, Sefa Can Saçilik, Ufuk Gündüz, Nurten Altinlar, Cumhur Çökmüş

Turkish Journal of Biology

Native-polyacrylamide gel electrophoresis (N-PAGE) was used to characterize Candida albicansstrains isolated from vaginal specimens of patients suffering from vaginitis in Ankara hospitals, Turkey. Three different protein band profiles were observed among these 38 C. albicansisolates when whole cell protein extracts of these strains were used in N-PAGE. 34 of the 38 isolates were shown to belong to a single group or clone with a dissemination percentage of 89, while three isolates belonged to another group with a percentage of 8. Furthermore only one C. albicans isolate was detected with a dissemination percentage of 3 and assigned to a third group. …

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Protein Profiles And Prevalence Of Methicillinresistant Staphylococcus Aureus (Mrsa) In Gülhanemilitary Academy Hospital In Turkey, Sefa Can Saçilik, Özlem Osmanağaoğlu, Özgül Kisa, Ahmet Başustaoğlu, Cumhur Çökmüş Jan 2000

Protein Profiles And Prevalence Of Methicillinresistant Staphylococcus Aureus (Mrsa) In Gülhanemilitary Academy Hospital In Turkey, Sefa Can Saçilik, Özlem Osmanağaoğlu, Özgül Kisa, Ahmet Başustaoğlu, Cumhur Çökmüş

Turkish Journal of Biology

The present study was undertaken to determine the relationship between Methicillin-Resistant Staphylococcus aureus (MRSA) strains isolated from various clinical specimens in Gülhane Military Medical Academy, in Ankara, Turkey. In 1997, at this hospital, the percentage of S. aureus among all nosocomial isolates was 20% and the percentage of MRSA among these S. aureus isolates was 51%. Both sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) and Native-Page (N-PAGE) were used for typing MRSA isolates which have common antibiotic susceptibility test results. MRSA isolates were also evaluated according to the whole-cell proteins (WCPs) and filtrate proteins (FPs). Using these methods, we concluded that …

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Antiviral Activity Of Some Plant Extracts On Thereplication Of Autographa Californicanuclearpolyhedrosis Virus, Ömer Ertürk, Zi̇hni̇ Demi̇rbağ, Ali̇ Osman Beldüz Jan 2000

Antiviral Activity Of Some Plant Extracts On Thereplication Of Autographa Californicanuclearpolyhedrosis Virus, Ömer Ertürk, Zi̇hni̇ Demi̇rbağ, Ali̇ Osman Beldüz

Turkish Journal of Biology

The effect of crude extracts, obtained from Aconitum nasutum, Daphne glomerata, Hypericum androsaemum, Laurus nobilis, Nerium oleander, Olea europaea, Prunus laurocerasus, Punica granatum, Rhododendron caucasicum, and Urtica dioica on the replication of Autographa californica nuclear polyhedrosis virus (AcNPV) grown in Spodoptera frugiperda cell culture were investigated by observing changes in cytophatic effects, progeny virus concentration and viral protein (polyhedrin) synthesis. We determined an inhibitory effect on cytophatic changes, virus concentration and synthesis of polyhedrin by utilizing the extract of A conitum nasutum, Hypericum androsaemum, Laurus nobilis, Rhododendron caucasicum, and Urtica dioica plants. On the other hand, the effects of Daphne …

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Blood Serum Proteins Of The Genus Mesocricetusnehring, 1898 (Mammalia: Rodentia) In Turkey, Reyhan Çolak, Nuri̇ Yi̇ği̇t, Ercüment Çolak, Mustafa Sözen, Şaki̇r Özkurt Jan 2000

Blood Serum Proteins Of The Genus Mesocricetusnehring, 1898 (Mammalia: Rodentia) In Turkey, Reyhan Çolak, Nuri̇ Yi̇ği̇t, Ercüment Çolak, Mustafa Sözen, Şaki̇r Özkurt

Turkish Journal of Biology

In this study, 18 live specimens collected from six localities in the distribution areas of Mesocricetus brandti and Mesocricetus auratus were examined using SDS-PAGE (sodium dodecyl sulphate-polyacrylamide gel electrophoresis). The findings showed that there is no difference in globulin and albumin proteins of M. brandti and M. auratus, and supported the claim that M. auratus is very closely related to M. brandti.

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Production Of Uricase Enzyme From Aspergillus Niger And Determination Of Some Factors Affecting The Activity, Fi̇gen Ertan, Erol Aksöz Jan 2000

Production Of Uricase Enzyme From Aspergillus Niger And Determination Of Some Factors Affecting The Activity, Fi̇gen Ertan, Erol Aksöz

Turkish Journal of Biology

This study was undertaken to obtain uricase enzyme from Aspergillus niger and also to determine the production conditions and the effects of some factors on enzyme activity. The results of the experiments, in consideration of the production of the uricase enzyme and the factors affecting the production, demonstrated that enzyme activity was maximum when the production time was 3 days at a temperature of 30ºC with the initial pH at 6.0 and inductor concentration at 0.1%. It was determined that ammonium compounds, which were added to the production medium, inhibited enzyme synthesis, and that MgS0_4 has an important role in …

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