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A Gene Expression Screen Identifies Earli1 As A Novel Vernalization-Responsive Gene In Arabidopsis Thaliana, Richard K. Wilkosz, Michael Schläppi Dec 2000

A Gene Expression Screen Identifies Earli1 As A Novel Vernalization-Responsive Gene In Arabidopsis Thaliana, Richard K. Wilkosz, Michael Schläppi

Biological Sciences Faculty Research and Publications

Vernalization promotes early flowering in late ecotypes of Arabidopsis thaliana. The mechanisms of vernalization are poorly understood. A subtractive hybridization approach was used to isolate vernalization-responsive genes from a late-flowering ecotype of Arabidopsis thaliana based on the premise that transcript levels of such genes would increase with cold treatment and remain high even after removal of the vernalization stimulus. EARLI1 is the first Arabidopsis gene shown to be stably activated by vernalization. The abundance of its RNA is progressively elevated by vernalization and remains high for at least 20 days at room temperature. The basal level of EARLI1 RNA …

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Do Timing And Pattern Of Myogenesis Correlate With Life History Mode In Anurans?, Matthew T. Smetanick, Rafael O. De Sá, Gary P. Radice Dec 2000

Do Timing And Pattern Of Myogenesis Correlate With Life History Mode In Anurans?, Matthew T. Smetanick, Rafael O. De Sá, Gary P. Radice

Biology Faculty Publications

The timing and pattern of myogenesis varies among anurans that have been studied and the different pat- terns may provide useful phylogenetic information. Specific myogenic markers have been described (Muntz, 1975; Kielbowna, 1981; Boudjelida & Muntz, 1987; Radice et al., 1989) and they can provide infor- mation on evolutionary changes for closely related lineages within a clade. For example, we previously com- pared first appearance of a muscle-specific protein, first twitch of axial muscle, onset of multinucleation within axial myotome, and first heartbeat in two pipid genera (Smetanick et al., 1999). We found that although the timing of myogenesis differed, …

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A Comparison Study Of Astm-Ifa (Icr) Method, The Pronetic Method And Dynal Method For Detection And Enumeration Of Giardia And Cryptosproidium In Source Waters In Kentucky, Lisa Curtis Hawkins Nov 2000

A Comparison Study Of Astm-Ifa (Icr) Method, The Pronetic Method And Dynal Method For Detection And Enumeration Of Giardia And Cryptosproidium In Source Waters In Kentucky, Lisa Curtis Hawkins

Morehead State Theses and Dissertations

A thesis presented to the faculty of the College of Science and Technology at Morehead State University in partial fulfillment of the requirements for the Degree of Master of Science in Biology by Lisa Curtis Hawkins on November 28, 2000.

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Somite Development In Zebrafish, Heather L. Stickney, Michael J.F. Barresi, Stephen H. Devoto Nov 2000

Somite Development In Zebrafish, Heather L. Stickney, Michael J.F. Barresi, Stephen H. Devoto

Biological Sciences: Faculty Publications

A full understanding of somite development requires knowledge of the molecular genetic pathways for cell determination as well as the cellular behaviors that underlie segmentation, somite epithelialization, and somite patterning. The zebrafish has long been recognized as an ideal organism for cellular and histological studies of somite patterning. In recent years, genetics has proven to be a very powerful complementary approach to these embryological studies, as genetic screens for zebrafish mutants defective in somitogenesis have identified over 50 genes that are necessary for normal somite development. Zebrafish is thus an ideal system in which to analyze the role of specific …

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H-Ns Controls Pap And Daa Fimbrial Transcription In Escherichia Coli In Response To Multiple Environmental Cues, C. A. White-Ziegler, A. Villapakkam, K. Ronaszeki, S. Young Nov 2000

H-Ns Controls Pap And Daa Fimbrial Transcription In Escherichia Coli In Response To Multiple Environmental Cues, C. A. White-Ziegler, A. Villapakkam, K. Ronaszeki, S. Young

Biological Sciences: Faculty Publications

A comparative study was completed to determine the influence of various environmental stimuli on the transcription of three different fimbrial operons in Escherichia coli and to determine the role of the histone-like protein H-NS in this environmental regulation. The fimbrial operons studied included the pap operon, which encodes pyelonephritis-associated pili (P pili), the daa operon, which encodes F1845 fimbriae, and the fan operon, which encodes K99 fimbriae. Using lacZYA transcriptional fusions within each of the fimbrial operons, we tested temperature, osmolarity, carbon source, rich medium, oxygen levels, pH, amino acids, solid medium, and iron concentration for their effects on fimbrial …

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Fast Transport Of Neurofilament Protein Along Microtubules In Squid Axoplasm, Veena Prahlad, Brian T. Helfand, George M. Langford, Ron D. Vale, Robert D. Goldman Nov 2000

Fast Transport Of Neurofilament Protein Along Microtubules In Squid Axoplasm, Veena Prahlad, Brian T. Helfand, George M. Langford, Ron D. Vale, Robert D. Goldman

Biology - All Scholarship

Using squid axoplasm as a model system, we have visualized the fast transport of non-filamentous neurofilament protein particles along axonal microtubules. This transport occurs at speeds of 0.5-1.0 microm/second and the majority of neurofilament particles stain with kinesin antibody. These observations demonstrate, for the first time, that fast (0.5-1.0 microm/second) transport of neurofilament proteins occurs along microtubules. In addition, our studies suggest that neurofilament protein can be transported as non-membrane bound, nonfilamentous subunits along axons, and that the transport is kinesin-dependent. Microtubule-based fast transport might therefore provide a mechanism for the distribution and turnover of neurofilament, and perhaps other cytoskeletal …

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Monitoring Bacterial Transport By Stable Isotope Enrichment Of Cells, William Holben, Peggy H. Ostrom Nov 2000

Monitoring Bacterial Transport By Stable Isotope Enrichment Of Cells, William Holben, Peggy H. Ostrom

Biological Sciences Faculty Publications

Understanding the transport and behavior of bacteria in the environment has broad implications in diverse areas, ranging from agriculture to groundwater quality, risk assessment, and bioremediation. The ability to reliably track and enumerate specific bacterial populations in the context of native communities and environments is key to developing this understanding. We report a novel bacterial tracking approach, based on altering the stable carbon isotope value (delta C-13) Of bacterial cells, which provides specific and sensitive detection and quantification of those cells in environmental samples. This approach was applied to the study of bacterial transport in saturated porous media. The transport …

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Dna Binding Properties Of The Arabidopsis Floral Development Protein Aintegumenta, Staci Nole-Wilson, Beth A. Krizek Nov 2000

Dna Binding Properties Of The Arabidopsis Floral Development Protein Aintegumenta, Staci Nole-Wilson, Beth A. Krizek

Faculty Publications

The Arabidopsis protein AINTEGUMENTA (ANT) is a member of a plant-specific family of transcription factors (AP2/EREBP) that share either one or two copies of an approximately 70 amino acid region called the AP2 repeat. DNA binding activity has been demonstrated previously for members of this family containing a single AP2 repeat. Using an in vitro selection procedure, the DNA binding specificity of the two AP2 repeat containing protein ANT was found to be 5'-gCAC(A/G)N(A/T)TcCC(a/g)ANG(c/t)-3'. This consensus site is much longer than sites recognized by proteins containing a single AP2 repeat and neither AP2 repeat of ANT was alone capable of …

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Effect Of Harvest And Effective Population Size On Genetic Diversity In A Striped Bass Population, Marilyn Diaz, David S. Wethey, James Bulak, Bert Ely Nov 2000

Effect Of Harvest And Effective Population Size On Genetic Diversity In A Striped Bass Population, Marilyn Diaz, David S. Wethey, James Bulak, Bert Ely

Faculty Publications

A major factor that contributes to loss of genetic variation in natural populations is a small effective population size. In species with a complex life history that involves overlapping generations and delayed maturity, the impact of infrequent annual reproductive bottlenecks is likely to be small because effective population size is defined by the number of individuals contributing to a generation and not to a single year-class. The striped bass Morone saxatilis is a longlived species with overlapping generations and age structure, whose recreational and commercial importance has made it a target of intense harvest.We analyzed allele frequency fluctuation among juvenile …

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From Hopeful Monsters To Homeotic Effects: Richard Goldschmidt's Integration Of Development, Evolution, And Genetics, Michael Dietrich Nov 2000

From Hopeful Monsters To Homeotic Effects: Richard Goldschmidt's Integration Of Development, Evolution, And Genetics, Michael Dietrich

Dartmouth Scholarship

Richard Goldschmidts research on homeotic mutants from 1940 until his death in 1958 represents one of the first serious efforts to integrate genetics, development, and evolution. Using two different models, Goldschmidt tried to show how different views of genetic structure and gene action could provide a mechanism for rapid speciation. Developmental systems were emphasized in one model and a hierarchy of genetic structures in the other. While Goldschmidt tried to find a balance between development and genetics, critics, such as Sewall Wright, urged him and eventually helped him incorporate population dynamics into his models as well. As such, the history …

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Biomechanics And Energetics In Aquatic And Semiaquatic Mammals: Platypus To Whale, Frank E. Fish Nov 2000

Biomechanics And Energetics In Aquatic And Semiaquatic Mammals: Platypus To Whale, Frank E. Fish

Biology Faculty Publications

A variety of mammalian lineages have secondarily invaded the water. To locomote and thermoregulate in the aqueous medium, mammals developed a range of morphological, physiological, and behavioral adaptations. A distinct difference in the suite of adaptations, which affects energetics, is apparent between semiaquatic and fully aquatic mammals. Semiaquatic mammals swim by paddling, which is inefficient compared to the use of oscillating hydrofoils of aquatic mammals. Semiaquatic mammals swim at the water surface and experience a greater resistive force augmented by wave drag than submerged aquatic mammals. A dense, nonwettable fur insulates semiaquatic mammals, whereas aquatic mammals use a layer of …

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A Family Of Developmentally Excised Dna Elements In Tetrahymena Is Under Selective Pressure To Maintain An Open Reading Frame Encoding An Integrase-Like Protein, Jill A. Gershan, Kathleen M. Karrer Nov 2000

A Family Of Developmentally Excised Dna Elements In Tetrahymena Is Under Selective Pressure To Maintain An Open Reading Frame Encoding An Integrase-Like Protein, Jill A. Gershan, Kathleen M. Karrer

Biological Sciences Faculty Research and Publications

Tlr1 is a member of a family of ~20-30 DNA elements that undergo developmentally regulated excision during formation of the macronucleus in the ciliated protozoan Tetrahymena. Analysis of sequence internal to the right boundary of Tlr1 revealed the presence of a 2 kb open reading frame (ORF) encoding a deduced protein with similarity to retrotransposon integrases. The ORFs of five unique clones were sequenced. The ORFs have 98% sequence conservation and align without frameshifts, although one has an additional trinucleotide at codon 561. Nucleotide changes among the five clones are highly non-random with respect to the position in the …

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Selection Of Alternative 5′ Splice Sites: Role Of U1 Snrnp And Models For The Antagonistic Effects Of Sf2/Asf And Hnrnp A1, Ian C. Eperon, Olga V. Makarova, Akila Mayeda, Stephen Munroe, Javier F. Cáceres, Daniel G. Hayward, Adrian R. Krainer Nov 2000

Selection Of Alternative 5′ Splice Sites: Role Of U1 Snrnp And Models For The Antagonistic Effects Of Sf2/Asf And Hnrnp A1, Ian C. Eperon, Olga V. Makarova, Akila Mayeda, Stephen Munroe, Javier F. Cáceres, Daniel G. Hayward, Adrian R. Krainer

Biological Sciences Faculty Research and Publications

The first component known to recognize and discriminate among potential 5′ splice sites (5′SSs) in pre-mRNA is the U1 snRNP. However, the relative levels of U1 snRNP binding to alternative 5′SSs do not necessarily determine the splicing outcome. Strikingly, SF2/ASF, one of the essential SR protein-splicing factors, causes a dose-dependent shift in splicing to a downstream (intron-proximal) site, and yet it increases U1 snRNP binding at upstream and downstream sites simultaneously. We show here that hnRNP A1, which shifts splicing towards an upstream 5′SS, causes reduced U1 snRNP binding at both sites. Nonetheless, the importance of U1 snRNP binding is …

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Effects Of Habitat Type And Drying On Ascogregarina Barretti (Eugregarinida : Lecudinidae) Infection In Aedes Triseritatus (Diptera : Culicidae), Stephen L. Van Rhein, Barry E. Flanary, Steven A. Juliano Nov 2000

Effects Of Habitat Type And Drying On Ascogregarina Barretti (Eugregarinida : Lecudinidae) Infection In Aedes Triseritatus (Diptera : Culicidae), Stephen L. Van Rhein, Barry E. Flanary, Steven A. Juliano

Faculty Publications – Biological Sciences

The intensity and prevalence of parasitism by Ascogregarina barretti (Vavra) in aedes triseriatus (Say) did not differ between tires and tree holes in field samples taken in September 1996. There was significant variation in the intensity of parasitism among containers that was not significantly cor-related with the pH, conductivity, or temperature of the container water. In an experiment manipulating habitat drying, treatments had a significant effect on A. barretti infection of Ae. triseriatus, only during midsummer in one of two years. Containers maintained at maximal volume had the lowest prevalence of parasitism, and containers that dried out had the greatest …

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Spaceflight Effects On Single Skeletal Muscle Fiber Function In The Rhesus Monkey, Robert H. Fitts, Dominique Desplanches, Janell Romatowski, Jeffrey J. Widrick Nov 2000

Spaceflight Effects On Single Skeletal Muscle Fiber Function In The Rhesus Monkey, Robert H. Fitts, Dominique Desplanches, Janell Romatowski, Jeffrey J. Widrick

Biological Sciences Faculty Research and Publications

The purpose of this investigation was to understand how 14 days of weightlessness alters the cellular properties of individual slow- and fast-twitch muscle fibers in the rhesus monkey. The diameter of the soleus (Sol) type I, medial gastrocnemius (MG) type I, and MG type II fibers from the vivarium controls averaged 60 ± 1, 46 ± 2, and 59 ± 2 µm, respectively. Both a control 1-G capsule sit (CS) and spaceflight (SF) significantly reduced the Sol type I fiber diameter (20 and 13%, respectively) and peak force, with the latter declining from 0.48 ± 0.01 to 0.31 ± 0.02 …

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Fast Transport Of Neurofilament Protein Along Microtubules In Squid Axoplasm, Veena Prahlad, Brian T. Helfand, George M. Langford, Ron D. Vale, Robert D. Goldman Oct 2000

Fast Transport Of Neurofilament Protein Along Microtubules In Squid Axoplasm, Veena Prahlad, Brian T. Helfand, George M. Langford, Ron D. Vale, Robert D. Goldman

Dartmouth Scholarship

Using squid axoplasm as a model system, we have visualized the fast transport of non-filamentous neurofilament protein particles along axonal microtubules. This transport occurs at speeds of 0.5-1.0 microm/second and the majority of neurofilament particles stain with kinesin antibody. These observations demonstrate, for the first time, that fast (0.5-1.0 microm/second) transport of neurofilament proteins occurs along microtubules. In addition, our studies suggest that neurofilament protein can be transported as non-membrane bound, nonfilamentous subunits along axons, and that the transport is kinesin-dependent. Microtubule-based fast transport might therefore provide a mechanism for the distribution and turnover of neurofilament, and perhaps other cytoskeletal …

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Does A Rigid Body Limit Maneuverability?, Jeffrey A. Walker Phd Oct 2000

Does A Rigid Body Limit Maneuverability?, Jeffrey A. Walker Phd

Faculty Publications

Whether a rigid body limits maneuverability depends on how maneuverability is defined. By the current definition, the minimum radius of the turn, a rigid-bodied, spotted boxfish Ostracion meleagris approaches maximum maneuverability, i.e. it can spin around with minimum turning radii near zero. The radius of the minimum space required to turn is an alternative measure of maneuverability. By this definition, O. meleagris is not very maneuverable. The observed space required by O. meleagris to turn is slightly greater than its theoretical minimum but much greater than that of highly flexible fish. Agility, the rate of turning, is related to maneuverability. …

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The Aquaculture Of Non-Endemic Species In Western Australia Silver Perch (Bidyanus Bidyanus), Tina Thorne, H. G. Brayford Oct 2000

The Aquaculture Of Non-Endemic Species In Western Australia Silver Perch (Bidyanus Bidyanus), Tina Thorne, H. G. Brayford

Fisheries Management Papers

The development of commercial aquaculture of a non-endemic species such as silver perch raises a number of important issues relating to translocation. These include the potential of the introduced species to: impact on genetic diversity; introduce disease; and impact on - the natural environment; and - the biodiversity of native species. This Management Paper has been drafted in liaison with the Department of Environmental Protection in accordance with the principles of rish assessment, taking into account the above issues. It is considered that the implementation of this policy will protect the natural environment and native fish species. It will also …

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Linear And Nonlinear Measures Predict Swimming In The Leech, C. J. Cellucci, Peter Brodfuehrer, H. Acera-Pozzi, E. Dobrovolny, J. Engler, R. Los, R. Thompson, Alfonso M. Albano Oct 2000

Linear And Nonlinear Measures Predict Swimming In The Leech, C. J. Cellucci, Peter Brodfuehrer, H. Acera-Pozzi, E. Dobrovolny, J. Engler, R. Los, R. Thompson, Alfonso M. Albano

Biology Faculty Research and Scholarship

Stimulation of a trigger interneuron of an isolated nerve cord preparation of the medicinal leech, Hirudo medicinalis, sometimes leads to swimming; sometimes it does not. We investigate signals transmitted in the ventral cord of the leech after stimulation and seek quantitative measures that would make it possible to distinguish signals that predict swimming from those that do not. We find that a number of linear as well as nonlinear measures provide statistically significant distinctions between the two kinds of signals. The linear measures are the time dependence of (i) the standard deviation and (ii) the autocorrelation function at a small …

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Facilitation May Buffer Competitive Effects: Indirect And Diffuse Interactions Among Salt Marsh Plants, Ragan M. Callaway, Steven C. Pennings Oct 2000

Facilitation May Buffer Competitive Effects: Indirect And Diffuse Interactions Among Salt Marsh Plants, Ragan M. Callaway, Steven C. Pennings

Biological Sciences Faculty Publications

Direct interactions among plant species may be highly modified by indirect or diffuse effects within a multispecies community. We investigated the direct and diffuse effects of two salt marsh perennials, Monanthechloe littoralis and Arthrocnemum subterminale, on winter annuals and the perennial herb Limonium californicum in a salt marsh in central California. In permanent plots, Monanthechloe had expanded substantially in the upper marsh over the past 13 yr, while Arthrocnemum and all annual species had decreased. These dynamics suggest that Monanthechloe may directly outcompete most other species in the upper marsh. In contrast, Arthrocnemum is known to facilitate some annual species. …

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Varying The Abundance Of O Antigen In Rhizobium Etli And Its Effect On Symbiosis With Phaseolus Vulgaris, K. Dale Noel, Lennart Scott Forsberg, Russell W. Carlson Oct 2000

Varying The Abundance Of O Antigen In Rhizobium Etli And Its Effect On Symbiosis With Phaseolus Vulgaris, K. Dale Noel, Lennart Scott Forsberg, Russell W. Carlson

Biological Sciences Faculty Research and Publications

Judged by migration of its lipopolysaccharide (LPS) in gel electrophoresis, the O antigen of Rhizobium etli mutant strain CE166 was apparently of normal size. However, its LPS sugar composition and staining of the LPS bands after electrophoresis indicated that the proportion of its LPS molecules that possessed O antigen was only 40% of the wild-type value. Its LPS also differed from the wild type by lacking quinovosamine (2-amino-2,6-dideoxyglucose). Both of these defects were due to a single genetic locus carrying a Tn5 insertion. The deficiency in O-antigen amount, but not the absence of quinovosamine, was suppressed by transferring into …

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Is Reproduction By Tree Swallows (Tachycineta Bicolor) Cost-Free?, Michael T. Murphy Oct 2000

Is Reproduction By Tree Swallows (Tachycineta Bicolor) Cost-Free?, Michael T. Murphy

Biology Faculty Publications and Presentations

We manipulated brood sizes of Tree Swallows (Tachycineta bicolor) in 1996 and 1997 to test for the existence of intra- and intergenerational costs of reproduction. Modal clutch size was six eggs, but experimental brood sizes ranged from two to nine young. Nestling starvation was higher in 1996 (and dependent on brood size) than in 1997, but in both years enlargement of brood size resulted in increased productivity. Nestling mass near fledging was negatively correlated with brood size, but tarsus length and wing chord were not. Food deliveries by parents increased steadily between broods of two to six young but then …

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A Conserved Mechanism For Post-Transcriptional Gene Silencing?, Eleanor M. Maine Sep 2000

A Conserved Mechanism For Post-Transcriptional Gene Silencing?, Eleanor M. Maine

Biology - All Scholarship

Proteins with homology to RNA-directed RNA polymerases function in post-transcriptional gene silencing: in quelling in the fungus Neurospora crassa, RNAi in the nematode Caenorhabditis elegans, and co-suppression in the mustard plant Arabidopsis thaliana. These findings are consistent with a conserved mechanism operating in these diverse species.

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Biology Department Publications: 2000, Sam Lindgren Sep 2000

Biology Department Publications: 2000, Sam Lindgren

Biology Department Bibliographies

This document is a noncomprehensive list of publications made by graduate students, post-doctoral researchers, and faculty members in the Biology Department in 2000. It indexes those publications available through major databases, and serves as an archival record of the Department’s robust scholarly contributions.

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An Unusual Pulse In Recruitment Of Two Reef Fishes In The Galápagos Islands Coincident With The 1997–1998 El Niño, Benjamin I. Ruttenberg Sep 2000

An Unusual Pulse In Recruitment Of Two Reef Fishes In The Galápagos Islands Coincident With The 1997–1998 El Niño, Benjamin I. Ruttenberg

Biological Sciences

No abstract provided.

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Regulation Of Sup Expression Identifies Multiple Regulators Involved In Arabidopsis Floral Meristem Development, Hajime Sakai, Beth A. Krizek, Steven E. Jacobsen, Elliot M. Meyerowitz Sep 2000

Regulation Of Sup Expression Identifies Multiple Regulators Involved In Arabidopsis Floral Meristem Development, Hajime Sakai, Beth A. Krizek, Steven E. Jacobsen, Elliot M. Meyerowitz

Faculty Publications

During the course of flower development, floral homeotic genes are expressed in defined concentric regions of floral meristems called whorls. The SUPERMAN ( SUP, also called FLO10 ) gene, which encodes a C2H2-type zinc finger protein, is involved in maintenance of the stamen/carpel whorl boundary (the boundary between whorl 3 and whorl 4) in Arabidopsis. Here, we show that the regulation of SUP expression in floral meristems is complex, consisting of two distinct phases, initiation and maintenance. The floral meristem identity gene LEAFY ( LFY ) plays a role in the initiation phase through at least two pathways, which …

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Religiopoiesis, Ursula Goodenough Sep 2000

Religiopoiesis, Ursula Goodenough

Biology Faculty Research

Religiopoiesis describes the crafting of religion, a core activity of humankind. Each religion is grounded in its myth, and each myth includes a cosmology of origins and destiny. The scientific worldview coheres as such a myth and calls for a religiopoietic response. The difficulties, opportunities, and imperatives inherent in this call are explored, particularly as they impact the working scientist.

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Have We Forgotten The Forest Because Of The Trees?, Stefan A. Schnitzer, Walter P. Carson Sep 2000

Have We Forgotten The Forest Because Of The Trees?, Stefan A. Schnitzer, Walter P. Carson

Biological Sciences Faculty Research and Publications

No abstract provided.

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Los Anfibios Del Monumento Natural Barro Colorado, Parque Nacional Soberania Y Areas Adyacentes, Rafael O. De Sá Sep 2000

Los Anfibios Del Monumento Natural Barro Colorado, Parque Nacional Soberania Y Areas Adyacentes, Rafael O. De Sá

Biology Faculty Publications

This book focuses on the batrachofauna of Barro Colorado Nature Monument. Soberania National Park and adjacent areas. It complements other recent publications on Neotropical amphibians such as those by Rodriguez and Duellman ( 1994) on Peru, Achaval and Olmos (1995) on Uruguay, and Meyer and Foster (1996) on Belize. The goal of these books is to reach a wider audience than the more technical batrachofaunal summaries-e.g., Frogs of Boracéia (Heyer 1990). Amphibians of Argentina (Cei 1985), and Frogs of the Genus Eleutherodactylus in Western Ecuador (Lynch and Duellman 1997). These geographically focused guides are especially useful to students, amateur biologists, …

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Molecular Cloning And Analysis Of The Modification Genes Of The Kpnbl Restriction- Modification System Of Klebsiella Pneumoniae Strain Gm236, Vernon Robert Chin Sep 2000

Molecular Cloning And Analysis Of The Modification Genes Of The Kpnbl Restriction- Modification System Of Klebsiella Pneumoniae Strain Gm236, Vernon Robert Chin

Loma Linda University Electronic Theses, Dissertations & Projects

The KpnBl restriction-modification (R-M) system has been recognized for some time in Klebsiella pneumoniae strain GM236. Previously the restriction subunit (hsdRkpmBI) was cloned and sequenced, based on the sequence infonnation the KpnBl RM system was suspected to be either a type I or type III R-M system In this project the modification genes of KpnBl were cloned into a pMECA plasmid by using a modification expression method. The modification genes were identified on an 8.2 kb EcoRl fragment from a chromosomal library of the GM236 strain. The complete 8.2 kb fragment was sequenced and …

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