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Protein Coevolution And Coadaptation In The Vertebrate Bc1 Complex, Kimberly Kay Baer

Theses and Dissertations

The cytochrome bc1 complex of the mitochondrial electron transport chain accomplishes the enzymatic reaction known as the modified Q-cycle. In the Q-cycle the bc1 complex transports protons from the matrix to the intermembrane space of the mitochondria, creating the proton gradient used to make ATP. The energy to move these protons is obtained by shuttling electrons from the coenzyme ubiquinol (QH2) to coenzyme ubiquinone (Q) and the mobile cytochrome c. This well studied complex is ideal for examining molecular adaptation because it consists of ten different subunits, it functions as a dimer, and it includes at least five different active …

Bioassessment And The Partitioning Of Community Composition And Diversity Across Spatial Scales In Wetlands Of The Bonneville Basin, Mary Jane Keleher

Theses and Dissertations

The Bonneville Basin encompasses an area that was covered by ancient Lake Bonneville and which today lies within the Great Basin province. The Bonneville Basin is distinguished geologically by its characteristic parallel north-south mountain ranges that are separated by broad, alluviated desert basins and valleys. Benches and other shoreline features of ancient Lake Bonneville prominently mark the steep, gravelly slopes of these ranges. Numerous artesian desert springs are present at the base of the mountains and in the valley floors that form various sizes of both isolated wetlands and wetland complexes. Many these wetlands are some of the most unique …

Description Of The Tadpole Of Leptodactylus Pustulatus Peters, 1870 (Anura: Leptodactylidae), Rafael O. De Sá, Reuber Brandão, Lorena Dall'ara Guimarães

Biology Faculty Publications

The external morphology and oral disc of the tadpole of Leptodactylus pustulatus Peters, 1870, are described and illustrated for Gosner stage 39. The internal oral anatomy was analyzed with SEM at Gosner stage 36 whereas chondrocranial anatomy is reported for Gosner stage 35. The overall characteristics do not depart from those known for the genus Leptodactylus and they particularly agree for those of the melanonotus species group. The labial tooth row formula is 2(2)/3. Observations are presented that suggest that L. pustulatus engages in larval parental care.

Resumo

São descritas a morfologia externa e o disco oral do girino de …

Regulation Of Meiotic Cohesion And Chromosome Core Morphogenesis During Pachytene In Drosophila Oocytes, Radhika S. Khetani, Sharon E. Bickel

Dartmouth Scholarship

During meiosis, cohesion between sister chromatids is required for normal levels of homologous recombination, maintenance of chiasmata and accurate chromosome segregation during both divisions. In Drosophila, null mutations in the ord gene abolish meiotic cohesion, although how ORD protein promotes cohesion has remained elusive. We show that SMC subunits of the cohesin complex colocalize with ORD at centromeres of ovarian germ-line cells. In addition, cohesin SMCs and ORD are visible along the length of meiotic chromosomes during pachytene and remain associated with chromosome cores following DNase I digestion. In flies lacking ORD activity, cohesin SMCs fail to accumulate at oocyte …

Assessing Causes Of Change In The Freshwater Mussels (Bivalvia: Unionidae) In The Black River, Ohio, Mark S. Lyons, Robert A. Krebs, Jeremy P. Holt, Louie J. Rundo, W. Zawiski

Biological, Geological, and Environmental Faculty Publications

Habitat destruction is believed to be the number one cause of the decline in unionid mussels. Around the world, cities, towns and agriculture alter the structure of watersheds, and the Black River in Ohio may be a typical example. We investigated the diversity and abundance of unionid mussels in this watershed and compared results to urbanization locations, to site-specific appearance of the habitat and to a 1997 fish survey, as host species are another factor important to the distribution of unionid mussels. Although shells were found for 21 species, only 11 of these species were found alive. Seven of the …

Host Resistance To Monilinia Vaccinii-Corymbosi In Flowers And Fruits Of Highbush Blueberry, Jeffery S. Lehman, Suzu Igarashi, Peter V. Oudemans

Biology and Earth Science Faculty Scholarship

Monilinia vaccinii-corymbosi, the causal agent of mummy berry disease, infects blueberry flowers via the gynoecial pathway. To describe the expression of host resistance in highbush blueberry (Vaccinium corymbosum), fungal growth in the styles and colonization of the locules were compared among five blueberry cultivars in a series of controlled greenhouse experiments. Styles were harvested 1 and 4 days postinoculation, and the length colonized by hyphae was determined using fluorescence microscopy. At 8 weeks after inoculation, fruit were harvested and scored for the presence of hyphae in the locules. The infection frequency of styles ranged from 0.33 to 0.71, and only …

The Phd Degree And Biblical Fundamentalism, William D. Stansfield

Biological Sciences

No abstract provided.

Description Of The First Chlamydopsinae (Coleoptera: Histeridae) From The Philippines, Michael S. Caterino, Alexey K. Tishechkin

Publications

The first two species of the obligate inquilinous subfamily Chlamydopsinae (Coleoptera: Histeridae) from the Philippines, Eucurtiopsis ashei (Luzon Island) and E. avis (Negros Island), are described and illustrated. Positions of the new species within the genus and potential biogeographic connection of the Philippine chlamydopsine fauna are discussed.

Keeping Insects At Bay: Beyond Deet, Thomas P. Rooney

Biological Sciences Faculty Publications

No abstract provided.

Comparison Of C. Elegans And C. Briggsae Genome Sequences Reveals Extensive Conservation Of Chromosome Organization And Synteny, Ladeana W. Hiller, Raymond D. Miller, Scott Everet Baird, Asif Chinwalla, Lucinda A. Fulton, Daniel C. Koboldt, Robert H. Waterston

Biological Sciences Faculty Publications

To determine whether the distinctive features of Caenorhabditis elegans chromosomal organization are shared with the C. briggsae genome, we constructed a single nucleotide polymorphism-based genetic map to order and orient the whole genome shotgun assembly along the six C. briggsae chromosomes. Although these species are of the same genus, their most recent common ancestor existed 80-110 million years ago, and thus they are more evolutionarily distant than, for example, human and mouse. We found that, like C. elegans chromosomes, C. briggsae chromosomes exhibit high levels of recombination on the arms along with higher repeat density, a higher fraction of intronic …

A Science Club Takes Action, Olivia Ledee, Anna Mosser, Tony Gamble, Greg Childs, Karen Oberhauser

Biological Sciences Faculty Research and Publications

No abstract provided.

Present Effects Of Past Wildfires On The Diets Of Stream Fish, Peter Koetsier, Quenton Tuckett, Joshua White

Biology Faculty Publications and Presentations

We investigated present indirect effects from a decade-old burn on the diets of stream fish. Based on soil instability and burn patterns from a 12–14-year-old wildfire complex, we separated 9 streams into 3 conditions: unburned, burned, and burned/scoured (i.e., experiencing a massive scour event 1 year post-burn). In the streams, we measured light levels and water temperatures, and we estimated fish density and biomass. We removed the digestive tracts from 9–15 rainbow trout (Oncorhynchus mykiss) from each stream and used a gravimetric procedure to analyze gut contents. Canopy cover development may dictate the composition of dietary items. Greater …

Direct And Indirect Effects Of Animal Detritus On Growth, Survival, And Mass Of Invasive Container Mosquito Aedes Albopictus (Diptera : Culicidae, Donald A. Yee, Banugopan Kesavaraju, Steven A. Juliano

Faculty Publications – Biological Sciences

Compared with plant detritus, animal detritus yields higher growth rates, survival, adult mass, and population growth of container-dwelling mosquitoes. It is unclear whether the benefit from animal detritus to larvae results from greater microorganism growth, direct ingestion of animal detritus by larvae, or some other mechanism. We tested alternative mechanisms by which animal detritus may benefit the invasive container-dwelling mosquito Aedes albopictus (Skuse) (Diptera: Culicidae). In the laboratory, larvae were reared under three conditions with access to 1) detritus, but where microorganisms in the water column were reduced through periodic flushing; 2) water column microorganisms, but larvae had no direct …

Taxonomy Of The Species Of Amphigerontia (Psocoptera : Psocidae) Of The Rocky Mountains Of The United States And Canada, Edward L. Mockford, Johannes E. Anonby

Faculty Publications – Biological Sciences

Five species of psocids of the genus Amphigerontia Kolbe 1880, are recorded from the Rocky Mountains of the United States and Canada. Amphigerontia longicauda, n. sp., is described. Amphigerontia petiolata (Banks) is recorded from the Rocky Mountains for the first time. The holotype of Amphigerontia infernicola (Chapman) is re-examined and described in detail. New characters for the separation of females of Amphigerontia bijasciata (Latreille) and Amphigerontia montivaga (Chapman) resulted in many new records of the latter species, previously known in the Rocky Mountains from only a single locality. Species-level characters are reviewed and several new ones are added. A key …

Comparative Functional Analysis Of Aquaporins/Glyceroporins In Mammals And Anurans, Carissa M. Krane, David L. Goldstein

Biology Faculty Publications

Maintenance of fluid homeostasis is critical to establishing and maintaining normal physiology. The landmark discovery of membrane water channels (aquaporins; AQPs) ushered in a new area in osmoregulatory biology that has drawn from and contributed to diverse branches of biology, from molecular biology and genomics to systems biology and evolution, and from microbial and plant biology to animal and translational physiology. As a result, the study of AQPs provides a unique and integrated backdrop for exploring the relationships between genes and genome systems, the regulation of gene expression, and the physiologic consequences of genetic variation. The wide species distribution of …

Prevalence And Diversity Of Avian Hematozoan Parasites In Asia: A Regional Study, Farah Ishtiaq, Eben Gering, Jon H. Rappole, Asad R. Rahmani, Yadvendradev V. Jhala, Carla J. Dove, Christopher M. Milensky, Storrs L. Olson, Mike A. Peirce, Robert C. Fleischer

Biology Faculty Articles

Tissue samples from 699 birds from three regions of Asia (Myanmar, India, and South Korea) were screened for evidence of infection by avian parasites in the genera Plasmodium and Haemoproteus. Samples were collected from November 1994 to October 2004. We identified 241 infected birds (34.0%). Base-on-sequence data for the cytochrome b gene from 221 positive samples, 34 distinct lineages of Plasmodium, and 41 of Haemoproteus were detected. Parasite diversity was highest in Myanmar followed by India and South Korea. Parasite prevalence differed among regions but not among host families. There were four lineages of Plasmodium and one of …

Sensory Modulation Of Crustacean Non-Giant Tailflipping, Sandra Y. Espinoza, Zen Faulkes

School of Integrative Biological & Chemical Sciences (Formerly Dept. of Biology)

As a first step in re-examining the modulation of non-giant tailflipping, we further investigate how the presence of weapons affects non-giant tailflipping. Removing both claws significantly changes the threshold for tailflipping (Krasne and Wine, 1975; Lang et al., 1977). Claw removal affects several variables at once, however; e.g., the animal’s mass; visual stimuli associated with having claws; tactile and proprioceptive cues. For example, a crayfish with claws that are present but useless might “bluff,” and may not tailflip away from a stimuli more than an intact animal.

Change In Nutritional Status Modulates The Abundance Of Critical Pre-Initiation Intermediate Complexes During Translation Initiation In Vivo, Chingakham Ranjit Singh, Tsuyoshi Udagawa, Bumjun Lee, Sarah Wassink, Hei He, Yasufumi Yamamoto, James T. Anderson, Graham D. Pavitt, Katsura Asano

Biological Sciences Faculty Research and Publications

In eukaryotic translation initiation, eIF2∙GTP–Met-tRNA_i^Met ternary complex (TC) interacts with eIF3–eIF1–eIF5 complex to form the multifactor complex (MFC), while eIF2∙GDP associates with eIF2B for guanine nucleotide exchange. Gcn2p phosphorylates eIF2 to inhibit eIF2B. Here we evaluate the abundance of eIFs and their pre-initiation intermediate complexes in gcn2 deletion mutant grown under different conditions. We show that ribosomes are three times as abundant as eIF1, eIF2 and eIF5, while eIF3 is half as abundant as the latter three and hence, the limiting component in MFC formation. By quantitative immunoprecipitation, we estimate that ∼ 15% of …

Monitoring Stress During Training, Olivita Couso

Seton Hall University Dissertations and Theses (ETDs)

The highly stressful job of law enforcement personnel, rescue workers and soldiers requires them to constantly put to the test their previous training. In order too respond effectively under pressure, the training of these personnel must be preformed under conditions that elicit pressure. The research proposed in this thesis evaluates established training regimens for the degree of stressfulness instilled in trainees from physiological (hypothalamic-pituitary adrenal axis, autonomic nervous system) and psychological perspectives (anxiety). Subjects participating in SWAT and military training were recruited, as well as subjects having no prior SWAT or military training. Individual physiological stress responses measured included heart …

Taxonomy Of The Species Of Amphigerontia (Psocoptera : Psocidae) Of The Rocky Mountains Of The United States And Canada, Edward L. Mockford, Johannes E. Anonby

Faculty Publications – Biological Sciences

Five species of psocids of the genus Amphigerontia Kolbe 1880, are recorded from the Rocky Mountains of the United States and Canada. Amphigerontia longicauda, n. sp., is described. Amphigerontia petiolata (Banks) is recorded from the Rocky Mountains for the first time. The holotype of Amphigerontia infernicola (Chapman) is re-examined and described in detail. New characters for the separation of females of Amphigerontia bijasciata (Latreille) and Amphigerontia montivaga (Chapman) resulted in many new records of the latter species, previously known in the Rocky Mountains from only a single locality. Species-level characters are reviewed and several new ones are added. A key …

Lifetime Reproductive Success Of Female Eastern Kingbirds (Tyrannus Tyrannus): Influence Of Lifespan, Nest Predation And Body Size, Michael T. Murphy

Biology Faculty Publications and Presentations

I report on the lifetime reproductive success (LRS) of female Eastern Kingbirds (Tyrannus tyrannus) in central New York. I investigated the major correlates of LRS and specifi cally tested the hypothesis that small body size yields reproductive benefi ts. Lifetime reproductive success varied widely: 15–20% of females failed to fledge young over their life, whereas 50% of young were fledged by 20% of females. Female lifespan varied between one and eight years, and females that died after one breeding season tended to be smaller-bodied than long-lived females (≥2 seasons). I therefore conducted analyses of LRS for the entire sample and …

Atplai Is An Acyl Hydrolase Involved In Basal Jasmonic Acid Production And Arabidopsis Resistance To Botrytis Cinerea, Xuemin Wang, Wenyu Yang, Shivakumar Devaiah, Xiangqing Pan, Giorgis Isaac, Ruth Welti

Biology Department Faculty Works

Intracellular phospholipase A2 (PLA2) plays an important role in regulating oxylipin biosynthesis in mammals, but the molecular and biochemical nature of intracellular PLA2 is not well understood in plants. Arabidopsis thaliana gene At1g61850 (AtPLAI) encodes a 140-kDa protein that is most similar to mammalian calcium-independent PLA2, and additionally contains leucine-rich repeats and Armadillo repeats. AtPLAI hydrolyzes phospholipids at both the sn-1 and sn-2 positions, but prefers galactolipids to phospholipids as substrates. Profiling of lipid species altered in response to the necrotrophic fungus Botrytis cinerea revealed decreases in the levels of phosphatidylglycerol and digalactosyldiacylglycerol, suggesting that hydrolysis of plastidic polar lipids …

Somatostatin Receptor Subtype 2 Is Functionally Expressed In Raw264.7 Cells, Matthew Sung

Seton Hall University Dissertations and Theses (ETDs)

Chronic inflammation left unchecked can be quite harmful to the tissue with the pro-inflammatory stimulus. It is marked by the recruitment and activation of leukocytes, including lymphocytes and macrophages with their subsequent proliferation and reactive oxygen species release. Macrophages are also one of the primary players in propagating the inflammatory response as they secrete pro-inflammatory cytokines to sustain local tissue responses. Current therapies for chronic inflammation include non-steroidal anti­ inflammatory drugs and glucocorticoids; however, both have various side effects and setbacks. Somatostatin is an endogenous hormone which inhibits cellular secretion and proliferation throughout the body. Somatostatin receptor activation is mediated …

Host Resistance To Monilinia Vaccinii-Corymbosi In Flowers And Fruits Of Highbush Blueberry, Jeffrey Lehman

Biology and Earth Science Faculty Scholarship

Monilinia vaccinii-corymbosi, the causal agent of mummy berry disease, infects blueberry flowers via the gynoecial pathway. To describe the expression of host resistance in highbush blueberry (Vaccinium corymbosum), fungal growth in the styles and colonization of the locules were compared among five blueberry cultivars in a series of controlled greenhouse experiments. Styles were harvested 1 and 4 days postinoculation, and the length colonized by hyphae was determined using fluorescence microscopy. At 8 weeks after inoculation, fruit were harvested and scored for the presence of hyphae in the locules. The infection frequency of styles ranged from 0.33 to 0.71, and only …

Comparative Chloroplast Genomics: Analyses Including New Sequences From The Angiosperms Nuphar Advena And Ranunculus Macranthus, Linda A. Raubeson, Rhiannon Peery, Timothy W. Chumley, Chris Dziubek, H. Matthew Fourcade, Jeffrey L. Boore, Robert K. Jansen

All Faculty Scholarship for the College of the Sciences

Background

The number of completely sequenced plastid genomes available is growing rapidly. This array of sequences presents new opportunities to perform comparative analyses. In comparative studies, it is often useful to compare across wide phylogenetic spans and, within angiosperms, to include representatives from basally diverging lineages such as the genomes reported here: Nuphar advena (from a basal-most lineage) and Ranunculus macranthus (a basal eudicot). We report these two new plastid genome sequences and make comparisons (within angiosperms, seed plants, or all photosynthetic lineages) to evaluate features such as the status of ycf15 and ycf68 as protein coding genes, the distribution …

Biblical Leprosy: Shedding Light On The Disease That Shuns, Alan L. Gillen

Faculty Publications and Presentations

Leprosy has terrified humanity since ancient times and was reported as early as 600 BC in India, China, and Egypt. Hansen’s disease is still a major health problem.

Disease is a constant reminder of just how much things have changed since God pronounced a curse on the earth. At first, everything was “very good,” but Adam’s sin brought death and decay into the world.

Seed Dispersal Limitation In A Neotropical Nutmeg, Virola Flexuosa (Myristicaceae): An Ecological And Genetic Approach, Kimberly Mae Holbrook

Dissertations

Seed dispersal contributes significantly to tropical forest maintenance, influencing processes, such as metapopulation dynamics and population persistence. Although several hypotheses have been debated regarding the contribution of seed dispersal to tropical forest diversity, recent work suggests that dispersal limitation is a major component in determining plant population patterns. This dissertation research broadly addresses hypotheses that seed dispersal behaviour of frugivores influences dispersal limitation of a Neotropical tree, Virola flexuosa (Myristicaceae). To test these hypotheses, I studied how different frugivores influenced seed dispersal of V. flexuosa at two sites in Amazonia Ecuador. General research objectives were to estimate fruit removal by …

Rhizobium Etli Ce3 Bacteroid Lipopolysaccharides Are Structurally Similar But Not Identical To Those Produced By Cultured Ce3 Bacteria, Wim D'Haeze, Christine Leoff, Glenn Freshour, K. Dale Noel, Russell W. Carlson

Biological Sciences Faculty Research and Publications

Rhizobium etli CE3 bacteroids were isolated from Phaseolus vulgaris root nodules. The lipopolysaccharide (LPS) from the bacteroids was purified and compared with the LPS from laboratory-cultured R. etli CE3and from cultures grown in the presence of anthocyanin. Comparisons were made of the O-chain polysaccharide, the core oligosaccharide, and the lipid A. Although LPS from CE3 bacteria and bacteroids are structurally similar, it was found that bacteroid LPS had specific modifications to both the O-chain polysaccharide and lipid A portions of their LPS. Cultures grown with anthocyanin contained modifications only to the O-chain polysaccharide. The changes to the O-chain polysaccharide consisted …

Localization Of Proteasomes And Proteasomal Proteolysis In The Mammalian Interphase Cell Nucleus By Systematic Application Of Immunocytochemistry, Andrea Scharf, Thomas Dino Rockel, Anna Von Mikecz

Biological Sciences Faculty Research & Creative Works

Proteasomes are ATP-driven, multisubunit proteolytic machines that degrade endogenous proteins into peptides and play a crucial role in cellular events such as the cell cycle, signal transduction, maintenance of proper protein folding and gene expression. Recent evidence indicates that the ubiquitin-proteasome system is an active component of the cell nucleus. A characteristic feature of the nucleus is its organization into distinct domains that have a unique composition of macromolecules and dynamically form as a response to the requirements of nuclear function. Here, we show by systematic application of different immunocytochemical procedures and comparison with signature proteins of nuclear domains that …

Biofouling Likely Serves As A Major Mode Of Dispersal For The Polychaete Tubeworm Hydroides Elegans As Inferred From Microsatellite Loci, J. B. Pettengill, D. E. Wendt, M. D. Schug, M. G. Hadfield

Biological Sciences

The polychaete tubeworm Hydroides elegans (Haswell) is a biofouling species with relatively limited larval dispersal. Four highly polymorphic microsatellite loci were used to make inferences about the migration and global population structure of 137 individuals from seven sub-populations located in the Atlantic, Pacific, and Indian Oceans and in the Mediterranean Sea. The results of the genetic analyses suggest minimal population sub-structure (F_st = 0.09). Estimates of pairwise F_st and migration rates using the coalescent-based method of MIGRATE suggest that there is little genetic differentiation between certain populations. Variation in relatedness among pairs of populations is consistent with a …