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Investigating The Ability Of Foxo1 To Maintain Stem Cells, Stella Aime Rios

Theses and Dissertations

Forkhead box O (Foxo) transcription factors are key regulators of stem cell identity and tumor aggressiveness. Prior work from our group and others demonstrates that Foxo factors maintain stem-like states across diverse biological systems; however, the mechanisms by which Foxo1 suppresses differentiation remain poorly defined.

Using C2C12 myoblasts as a model, we investigated whether Foxo1 preserves stem-like programs through regulation of the nuclear pore protein Nup210. RNA interference was used to generate Foxo1 knockdown cells, followed by qRT-PCR, RNA-sequencing, and fluorescence microscopy. Transcriptomic analysis revealed widespread gene expression changes involving metabolic, mitochondrial, and transcriptional pathways. Notably, Nup210 expression was significantly …

Resource Allocation Trade-Offs Between Longevity And Reproduction In Daphnia Magna, Amelia Pearson

Electronic Theses and Dissertations

Although trade-offs between longevity and reproduction have been described for a long time, the genetic mechanisms by which organisms modulate allocation of resources between these two essential processes remain poorly understood, due to their complex nature and their modification by existing nutritional states and environmental cues. Daphnia magna represents a valuable model for investigation in this area due to their moderate lifespan and cyclic parthenogenetic mode of reproduction, allowing for the easy study of gene by environment interactions. This dissertation first investigates the change in mortality, reproduction, activity, and gene expression when fully fed Daphnia and calorically restricted Daphnia are …

Searching Harder For The Darter: Evaluating Endangered Yellowcheek Darter (Nothonotus Moorei) Status And Drought Responses, Kearstin Findley

Graduate Theses and Dissertations

Freshwater ecosystems are one of the most imperiled habitats across the world with biodiversity declining rapidly. There are many stressors contributing to freshwater species declines including habitat loss and degradation, land-use changes, anthropogenic water demands, changes in precipitation and weather extremes, and pollution. Current environmental projections predict that these stressors will continue to impact freshwater ecosystems to greater extents moving forward. Stream drying from drought conditions increasing in intensity and frequency is especially concerning in northern Arkansas where seasonal drought is typical in the summer and fall months. With increased drying conditions, the elimination of shallow-water riffle habitats in streams …

Can Carpobrotus Edulis Cause Significant Changes To The Soil Microbiome?, Samantha J. Cowan

Electronic Theses, Projects, and Dissertations

Invasive species cause significant damage to ecosystem biodiversity and can cost billions of dollars to restore native species (Van der Wal et al, 2008; Diagne et al., 2021).  In certain instances, even if the invasive species are removed, they can leave legacy effects on the environment that make restoration more challenging (Conser and Connor, 2008; Elgersma et al., 2011). Carpobrotus edulis is an invasive species of plant that is known to cause changes to its environment such as affecting the soil nitrogen cycle and the pH, potentially leading to persistent changes in the soil microbiome and causing native species to …

Wrky Transcription Factors: Integral Regulators Of Defence Responses To Biotic Stress In Crops, Dongjiao Wang, Ruize Zhang, Wenhui Zou, Yuanyuan Zhang, Wanying Zhao, Tingting Sun, Qibin Wu, Zheng Qing Fu, Youxiong Que

Faculty Publications

Crops are continually challenged by biotic stresses, including fungal, bacterial and viral pathogens and insect pests, which cause substantial yield and quality losses worldwide. WRKY transcription factors constitute a plant-specific and functionally diverse family that is central to immune regulation. Recent advances in genomic resources and multi-omics approaches have accelerated the identification and functional characterisation of WRKYs in crops. This review summarises the structural features and classification of WRKY genes and their genome-wide distribution across crop species. It also synthesises WRKY-centred regulatory modules that mediate resistance to major classes of biotic stress. In antifungal defence, WRKYs reinforce pattern- and effector-triggered …

Vitaltitration Predicts Fermentation Performance Of Phylogenetically Distinct Saccharomyces Cerevisiae Yeast Strains, Samantha White Ms

All Student Scholarship

Accurate assessment of yeast physiological health is essential for achieving consistent, efficient, and predictable beer fermentations. Although yeast vitality is more predictive of fermentation performance than viability alone, no universally adopted vitality assay currently exists in the brewing industry. This thesis investigates vitaltitration (VT) as a rapid, robust, and brewery-feasible method for quantifying yeast vitality and predicting fermentation performance. We hypothesize that VT provides a reliable measure of metabolic competence that correlates with fermentation kinetics across brewery-relevant yeast strains. To test this hypothesis, VT was evaluated alongside Acidification Power (AP) in structured serial-fermentation experiments in two phylogenetically distinct brewing yeasts: …

Volume 17, Christian O’Neill, Kyara Greene, Savva Sidorov, Laura Bisaillon, Luke Clemmer, Hannah Gordon, Kitt Benson, Taylor Blount, Rachel Danzitz, Nicholas Duellman, Chase Gionis, Hima Fernando, Seth Franzyshen, Onyx Gonzalez, Bryan Lin, Samantha Start, Ysabel Wells, Maggie Duncan

Incite: The Journal of Undergraduate Scholarship

Introduction Dr. Amorette Barber, Director, Office of Student Research

From the Editor Dr. Hannah Dudley-Shotwell

Cover Artist’s Statement Maggie Duncan

On Mentoring Dr. Yulia Uryadova

Ukrainian Resistance in the Face of Russification: Nestor Makhno and Anarchism

by Christian O’Neill

Life Vest by Kyara Greene

Isolation and 16S rRNA Identification of Bacteria from Fire Department Connection Pipe by Savva Sidorov

The Effectiveness of Planned Exercise in Reducing ADHD Symptoms in Children by Laura Bisaillon & Luke Clemmer

Linguistic Analysis on Confidence and Communication Strategies with Disparities Between Sign Fluency and Hearing Impairment by Hannah Gordon

Freedmen in Indian Territory by Kitt …

From Antigen Presentation To Tumor Control: Mechanisms Of Type I Conventional Dendritic Cell-Based Cancer Vaccines, Josue E. Pineda

Dissertations and Theses (Open Access)

Type 1 conventional dendritic cells (cDC1s) are important for generating and sustaining antitumor immunity. Accordingly, the abundance of cDC1s in human tumors correlates with improved outcomes in cancer. Capitalizing on this role, we previously demonstrated that vaccination with in vitro-derived murine cDC1s elicits durable tumor control in multiple preclinical models; however, the immunological mechanisms underlying the efficacy of cDC1 vaccination remain unclear. Here, we examined whether in vitro-derived cDC1s resemble tumor-infiltrating DC populations and whether MHC-I and MHC-II antigen presentation contribute to cDC1-mediated tumor control following vaccination in melanoma.

As expected, MHC-I- or MHC-II-deficiency had minimal impact on …

Effects Of Tourist Feeding On The Gut Microbiome And Ecophysiology Of Endangered Northern Bahamian Rock Iguana (Cyclura Cychlura), Erin Lucy Lewis

All Graduate Theses and Dissertations, Fall 2023 to Present

The lifespan and reproduction of an animal depends on maintaining a healthy physiology. The proportion of individuals that survive to an age at which procreation can occur is important to long-term stability of populations and contributes to species survival. Health in an animal is measured in a variety of ways, including through monitoring of the body’s physiological systems, as well as its ability to respond to invading pathogens, and through the different types of bacteria present in the gut. Relationships among those different health metrics have been discovered in recent years and have been found to play important roles in …

Mechanisms Of Life History Trade-Offs In The Evolution Of Eusociality, Frances Katherine Hunter

All Graduate Theses and Dissertations, Fall 2023 to Present

When faced with stressors such as pathogen infection, organisms must invest energy in defense or risk death. Because resources are limited, mounting an immune response often comes at the expense of other costly activities such as reproduction. The costs of immunity are shaped by factors including behavior, nutrition, age, and evolutionary history.

Social insects (e.g., ants, bees, and wasps) offer a powerful system for studying these trade-offs because queens in these colonies appear to escape them—living long lives while reproducing prolifically. Bees, which include species that range from those which nest solitarily to those with complex social systems and many …

Enhancing Walleye (Ogaa) Recruitment: Evaluating Assisted Reproduction As A Mitigation Strategy, Douglass Keiser

Biology Graduate Theses

Over the past several decades, cold- and cool-water fish populations across the Upper Midwest, including Minnesota and Wisconsin, have shown troubling signs of decline. Among the most affected is the walleye/Ogaa (Sander vitreus), a species deeply embedded in the ecological balance, cultural identity, and economic vitality of the region, and central to the lifeways, treaty-reserved rights, and resource stewardship of Tribal Nations, including Ojibwe communities across the Upper Midwest. Despite ongoing stocking efforts and harvest regulations, many lake systems have experienced persistent declines in natural recruitment, prompting resource agencies to investigate the underlying causes. One such system is …

Decomposition Study Using Pigs In Southeastern Nebraska During The Fall And Winter Seasons, Ryan Bodine

Department of Anthropology: Theses and Student Research

When an individual is found deceased, one of the tools used in the identification process is estimating the post-mortem interval (PMI). This estimate is used to determine how long an individual has been deceased once their remains are found. To estimate an accurate PMI, knowing how decomposition is affected by external factors is key. The transition period from fall to winter in southeastern Nebraska consists of fluctuating temperatures as the seasons transfer from fall to winter. This thesis investigates how decomposition is affected during this transition period while also looking at two different environments that are common in Nebraska, prairies …

Exploring The Biocultural Profile Of The Undocumented Populations Of The Sonoran Corridor: A Skeletal Assessment Of The Prevention Through Deterrence Strategy, Mary A. Vaughn

Department of Anthropology: Theses and Student Research

Over the last thirty years the mortality rate of the undocumented population at the US-Mexico border has risen, requiring methods distinguishing US-born individuals from immigrants to improve the likelihood of identification and repatriation through foreign consulates. Forensic anthropologists with the Pima County Office of the Medical Examiner (PCOME) have advocated for the use of the biocultural profile, combining the biological profile with factors including oral health, osteological stress, and personal effects. The biocultural profile serves two purposes, functioning both as a tool for identification and with which to document structural inequality within communities of origin.

This study examines changes in …

Nadh Flim Reveals Cellular Bioenergetics And Mitochondrial Morphology During Drosophila Convergent Extension, Maria Espana Pena

Graduate Theses and Dissertations

Mitochondria are dynamic organelles that can fragment or fuse to support different bioenergetic demands and cellular processes, although the role of mitochondrial fission and fusion during embryonic development is not well understood. Fluorescence lifetime imaging (FLIM) of the mitochondrial cofactor NADH can be used to visualize mitochondrial networks and infer aspects of cellular bioenergetics in a label-free manner. We used NADH FLIM to test whether germband cells undergo changes in cellular metabolism during Drosophila convergent extension (CE)––a process in which hundreds of epithelial cells undergo coordinated intercalation to elongate the embryo. Contrary to our expectations, we did not observe significant …

The Delivery Dilemma: Unintended Consequences Of Cesarean Sections, Cecilia Odegard, Ellie Rose Piette

Celebrating Scholarship and Creativity Day (2018-)

Cesarean sections (C-sections) are a major surgical procedure intended to minimize the rate of maternal and infant mortality due to complications during birth. While they were originally intended to only be performed when medically necessary, rates of non-medically indicated C-sections have increased significantly. This poster examines the unintended consequences (UICs) that are associated with C-section delivery, focusing on both short-term and long-term effects. A major focus is how C-sections disrupt early microbial exposure during birth, which plays an important role in the development of the infant gut microbiome and immune system. Research has shown that infants born via C- section …

Climate Change-Related Effects On Carbon Emissions And Net Ammonification Across Lowland To Upland Soils From A High-Latitude Coastal Ecosystem, Jenna M. Ross, Karen H. Beard, Katharine C. Kelsey, Matteo Petit Bon, A. Joshua Leffler

Wildland Resources Faculty Publications

Warming, sea-level rise, and changing herbivory patterns are affecting ecosystem processes in northern coastal soils, including carbon and nitrogen cycling, although their combined effects are poorly understood. We studied the impact of these changes in a full-factorial microcosm experiment using four distinct soil types from the Yukon-Kuskokwim Delta of western Alaska. Soil was collected from three coastal wetlands, which differ in flooding frequency and use by migratory geese, and one rarely-flooded upland tundra used by geese in late summer. Microcosms containing these soils were incubated for 16 weeks at 10°C or 18°C, with or without three short-term floods, and with …

Survival Of The Fittest: Extremophilic Actinomycetes As The Future Of Antibiotic Treatment, Kyley Nelson, Ben Reddan

Celebrating Scholarship and Creativity Day (2018-)

The antibiotic resistance crisis is an emerging global health threat. In 2019 alone, 1.27 million deaths were attributed to antibiotic-resistant pathogens, and researchers predict the problem will worsen without intervention. Methicillin-Resistant Staphylococcus aureus (MRSA) has become a primary model for this crisis because it utilizes genomic adaptations to bypass β-lactam antibiotics. Although temperate actinomycetes have historically provided the majority of clinical antibiotics, recent bioprospecting has revealed that extremophilic actinomycetes represent a critical untapped resource. These organisms exhibit several adaptations to thrive in extreme environments, which results in the production of novel secondary metabolites and resilient extracellular enzymes. This literature review …

Discovering The Genetics Underlying Speciation Traits In Heuchera (Coral Bells), Tajinder Singh

Honors Theses

Hybridization is a key evolutionary mechanism for generating biological diversity, ultimately shaping diversification and adaptation of plant lineages in natural and agroecosystems.  Our understanding of the frequency, distribution, and significance of hybridization is rapidly increasing, but the external driving forces that dictate why certain species hybridize, and others do not remain obscure. The physical traits that shape gene flow and trait sharing among species are likely to be among the key factors that directly control hybridization. Gene flow in most plants is mediated by pollinating insects and thus by pollination syndromes, which are suites of floral traits that have evolved …

The Big Picture Of The Small Intestine, Reagan Kangas

Celebrating Scholarship and Creativity Day (2018-)

Histology is the study of microscopic tissue structures and is used to understand the function of tissues and visualize the cellular organization within them. It is an important tool in biology and medicine, used for diagnosing disease (i.e., cancers, infections, inflammatory diseases, etc.) and studying organ structure and function. The goal of this poster is to compare preparation and staining techniques, specifically paraffin versus frozen sectioning methods, along with Masson’s trichrome versus hematoxylin and eosin (H&E) staining. These methods differ in processing time, tissue preservation, and the level of detail they reveal under a microscope. This project uses small intestines …

Survival Of The Fittest: Extremophilic Actinomycetes As The Future Of Antibiotic Treatment, Ben Reddan, Kyley Nelson

Celebrating Scholarship and Creativity Day (2018-)

The antibiotic resistance crisis is an emerging global health threat. In 2019 alone, 1.27 million deaths were attributed to antibiotic-resistant pathogens, and researchers predict the problem will worsen without intervention. Methicillin-Resistant Staphylococcus aureus (MRSA) has become a primary model for this crisis because it utilizes genomic adaptations to bypass β-lactam antibiotics. Although temperate actinomycetes have historically provided the majority of clinical antibiotics, recent bioprospecting has revealed that extremophilic actinomycetes represent a critical untapped resource. These organisms exhibit several adaptations to thrive in extreme environments, which results in the production of novel secondary metabolites and resilient extracellular enzymes. This literature review …

Sex Or Survival: Does Wounding Elicit A Somatic Reproductive Tradeoff In Narnia Femorata Males?, Lillian E. Burbulis

Departmental Honors & Graduate Capstone Projects

Animals must partition resources into different life history traits resulting in tradeoffs because resources are finite. A tradeoff is the allocation of resources to one trait over another. Theory states that energy is allocated in a hierarchical fashion. For example, somatic maintenance should be a higher priority than reproduction, resulting in more resources being allocated to somatic maintenance compared to reproductive traits. The metabolic investment hypothesis states that energy must follow a hierarchical order prioritizing somatic maintenance over reproduction. Another hypothesis for energy allocation is the terminal investment hypothesis. This hypothesis states that energy will be allocated to reproduction over …

A Two-Phase Hippocampal Code Linking Time And Distance Estimation To Action, Raphael Heldman

Electronic Theses and Dissertations

A critical component of spatial navigation is the ability to estimate elapsed time or distance traveled. How the brain generates such estimates and links them to behavior remains unclear. In this thesis, we identify a previously unrecognized population coding strategy for time or distance in hippocampal CA1, a region critical for navigation and memory. Using novel behavioral paradigms in head-fixed mice combined with electrophysiological recordings, we show that this code is carried by two functionally distinct subpopulations of pyramidal neurons, termed PyrUp and PyrDown, whose firing rates evolve in opposite directions during navigation. Both populations exhibit a common two-phase dynamic: …

Bioactive Constituents And Pharmacological Activities Of Curcuma Zedoaria: A Mini-Review, Abdulmutalib Allaq, Muneer M. Saleh Mohammed Saleh Alsayadi, Mustapha Salihu, Hasan M. Agha, Amena Hassan Hassan Gheeth, Fatimah Salim, Norrizah Jaafar Jaafar Sidik

AUIQ Complementary Biological System

Curcuma zedoaria Rosc. (white turmeric) is a rhizomatous medicinal plant in the Zingiberaceae family, widely used in traditional Asian medicine. In recent years, increasing scientific attention has focused on its diverse pharmacological properties and bioactive constituents. This mini review summarises current knowledge of the phytochemical composition and biological activities of C. zedoaria, with particular emphasis on its antimicrobial, antioxidant, anti-inflammatory, and anticancer effects. Phytochemical investigations using analytical techniques such as GC–MS and HPLC have revealed the presence of numerous bioactive compounds, including sesquiterpenes (example, germacrone, curzerenone, and curdione) and curcuminoids, which contribute to its therapeutic potential. Experimental studies demonstrate …

Evaluation Of Circulating Hpg80, Ca15-3, Estrogen And Progesterone As Biomarkers In Breast Cancer Patients, Safa Khaldoon, Emad Yousif, Khawla A. Kasar, Muna Bufaroosha, Rahimi Yusop

AUIQ Complementary Biological System

It is very important to find reliable biomarkers for early detection and monitoring of treatment response because breast cancer is still one of the most common cancers in women around the world. The goal of this study was to find out how clinically important different biochemical markers are in Iraqi women with breast cancer. Some of these signs are progesterone (PG), oestrogen (E2), cancer antigen 15-3 (CA15-3), and circulating progastrin (hPG80). There were 100 women in this study, and they were all between the ages of 20 and 70. There were three groups of people who took part: a healthy …

Synergistic Endocytic Receptor Ligand Receptor Protein 2 In T2dm, Wafaa Raji Alfatlawi

AUIQ Complementary Biological System

Purpose: The glycoprotein molecule known as megalin is present on the proximal renal tubular epithelial cells. With an emphasis on megalin and cubilin levels in T2DM and IR patients, an area that obviously needs more research, this study attempts to give an outline of current knowledge of megalin function in relation to vitamin D and its metabolite (vitamin D receptor and vitamin D binding protein). Materials and Methods: This was a case control comparative study conducted at a endocrine and diabetes center in AL-Imamain Kadumain teaching hospital in Baghdad-Iraq. Study subjects were divided into three groups: 25 with T2DM with …

Beyond Beneficial Margins: Four Mechanisms Linking Border Vegetation To Pest Dynamics, Jorge F. M. Cardoso, Fabiane M. Mundim

Biology Student Research

Vegetated field borders are widely promoted as tools to enhance biodiversity and strengthen biological control in agroecosystems. However, their role in pest dynamics remains conceptually fragmented and empirically inconsistent. Here, we develop a unified framework explaining how crop border vegetation influences pest populations through four interlinked ecological mechanisms. First, borders act as host reservoirs and selective filters, providing alternative hosts and overwintering habitat that enhance pest persistence across crop cycles. Second, borders modify pest colonization dynamics by shaping movement, aggregation, and host-location behavior at crop edges. Third, borders restructure multitrophic networks, simultaneously supporting natural enemies, alternative prey, vectors, and pathogens, …

Expanding The Fossil Record Of Hippopotamodon (Suidae, Artiodactyla) From The Hasnot Area, Siwalik, Pakistan, Muhammad Ilyas, Rana Mehroz Fazal, Sayyed Ghyour Abbas, Misbah Faiz, Kiran Aftab, Abdul Ghaffar, Khizar Samiullah, Luke Holbrook

STEM Student Research Symposium Posters

The suid genus Hippopotamodon with its type species H. sivalense, is well known from Upper Miocene deposits in Eurasia. Its taxonomic history has been characterized by considerable instability, including early misassignments to genera such as Sus and Potamochoerus, and subsequent reclassifications involving Propotamochoerus, Dicoryphochoerus, and Microstonyx. This confusion primarily resulted from an overreliance on variable dental and canine morphology. Subsequent systematic revisions attempted to resolve these issues by proposing synonymies among these taxa, although debate has continued regarding the relationship between Hippopotamodon and Microstonyx. Recent work supports the synonymy of these taxa based on shared diagnostic features across Eurasian material. …

Discovery And Characterization Of Three Novel Bacteriophages Infecting Arthrobacter Globiformis: Crescenzo (Ay), Gerri43 (Fr), & Roberts (Fr), Gabrielle Achinko, Maximillan Castro, Evan Chernyenko, Kelley Crescenzo, Natalie Horchuck, Jessica Lee, Mattie Mcmahon, Heidi Meehan, Sandra Orjih, Kanishka Poudel, Kaitlyn Roberts, Alex Bogush, Matthew Farber, Marina Bogush

STEM Student Research Symposium Posters

Bacteriophages are viruses that infect bacteria and are increasingly important in applications ranging from agriculture and food safety to phage therapy. In this study, we isolated and characterized three novel phages, Gerri43, Crescenzo, and Roberts, from soil samples collected in Greenwich, New Jersey, and from the Rowan University campus in Glassboro, New Jersey, using Arthrobacter globiformis as the host bacterium. Phages were recovered using standard enrichment and purification approaches. After initial isolation, multiple rounds of plaque purification and serial dilution were performed to obtain clonal populations. High-titer lysates were then prepared by flooding webbed plates. Electron microscopy showed that all …

Using The Flexon Approach To Rnai To Identify Essential Genes That Affect Gonadal Development In C. Elegans, Joyce S. Lefevers

Undergraduate Theses

To elucidate the molecular role of essential genes in the gonadal development of Caenorhabditis elegans (C. elegans) it is necessary to utilize specialized molecular techniques such that the phenotypic effects of gene knockdown can be studied while the viability of the organism is preserved. Shaffer and Greenwald (2022) developed the floxed exon (flexon), a tool that improves upon previous approaches to spatiotemporal control of gene expression. The flexon subunit is made up of an artificial exon with a stop cassette flanked by artificial introns which, when inserted into a gene of interest, prevents the expression of that gene. …

Neurological Phenotypes Of Myotonic Dystrophy Type 1 In Drosophila Melanogaster, Carleigh M. Wood

Departmental Honors & Graduate Capstone Projects

This research investigated Drosophila melanogaster’s ability to serve as a model organism for the neurological phenotypes of Myotonic Dystrophy Type I (DM1). DM1 is caused by a CTG repeat expansion in the DMPK gene. DM1 leads to symptoms of muscle weakness and wasting, as well as decreased cerebral volume. This study aimed to determine whether DM1-affected Drosophila exhibit neurological changes, as has been observed in humans. Healthy control (CUG20) and DM1-affected Drosophila (CUG250) were generated using classical genetics. Both groups were aged three weeks prior to dissection and imaged through fluorescent microscopy. It was predicted that Drosophila melanogaster affected by …