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A Discourse On The Public Nature Of Research In Contemporary Life Science: A Law-Policy Proposal To Promote The Public Nature Of Science In An Era Of Academia-Industry Integration, Michael J. Malinowski

Journal Articles

This article addresses the impact of integration of academia, industry, and government on the public nature of research. The article concludes that, while the integration has benefited science immensely, regulatory measures should be taken to restore the public nature of research in an age of integration.

A Redescription Of Three Species Of Monoecocestus (Cestoda: Anoplocephalidae) Including Monoecocestus Threlkeldi Based On New Material, Terry R. Haverkost, Scott Lyell Gardner

Scott L. Gardner Publications

Because we have new and detailed data on species in the genus, herein we redescribe both Monoecocestus macrobursatus and M. minor based on existing museum specimens. We also redescribe M. threlkeldi (Parra, 1952) using material collected in Bolivia. Based on the specimens representing M. thelkeldi, we affirm that Perutaenia Parra, 1953 should remain a junior synonym of Monoecocestus.

Sex Effects On Life Span And Senescence In The Wild When Dates Of Birth And Death Are Unknown, Felix Zajitschek, Chad Brassil, Russell Bonduriansky, Robert C. Brooks

School of Biological Sciences: Faculty Publications

Males and females allocate and schedule reproductive effort in very different ways. Because the timing and amount of reproductive effort influence survival and thus the optimization of life histories, mortality and senescence are predicted to be sex specific. However, age-specific mortality rates of wild animals are often difficult to quantify in natural populations. Studies that report mortality rates from natural populations are, therefore, almost entirely confined to long-lived, easy-to-track species such as large mammals and birds. Here, we employ a novel approach using capture–mark–recapture data from a wild population of black field crickets (Teleogryllus commodus) to test for …

Blood Sampling Reduces Annual Survival In Cliff Swallows (Petrochelidon Pyrrhonota) -- La Toma De Muestras De Sangre Reduce La Supervivencia Anual En Petrochelidon Pyrrhonota, Charles R. Brown, Mary Bomberger Brown

School of Biological Sciences: Faculty Publications

Researchers commonly collect blood samples from wild birds, and most workers assume that blood sampling has no adverse effect on the birds’ survival. Few studies, however, have done controlled comparisons among bled and non-bled individuals and estimated survival using modern statistical methodology. We used a data set on Cliff Swallows (Petrochelidon pyrrhonota) that included 2,945 bled and 7,822 non-bled birds captured at the same times and sites in southwestern Nebraska from 1986 to 2006 to estimate annual survival and recapture probabilities of each group. Blood was collected with brachial venipuncture in amounts varying from 0.3% to 1.2% of …

Something Old, Something New, Something Borrowed; How The Thermoacidophilic Archaeon Sulfolobus Solfataricus Responds To Oxidative Stress, Walid S. Maaty, Blake Wiedenheft, Pavel Tarlykov, Nathan Schaff, Joshua Heinemann, Jim Robinson-Cox, Jacob Valenzuela, Amanda Dougherty, Paul H. Blum, C. Martin Lawrence, Trevor Douglas, Mark J. Young, Brian Bothner

School of Biological Sciences: Faculty Publications

To avoid molecular damage of biomolecules due to oxidation, all cells have evolved constitutive and responsive systems to mitigate and repair chemical modifications. Archaea have adapted to some of the most extreme environments known to support life, including highly oxidizing conditions. However, in comparison to bacteria and eukaryotes, relatively little is known about the biology and biochemistry of archaea in response to changing conditions and repair of oxidative damage. In this study transcriptome, proteome, and chemical reactivity analyses of hydrogen peroxide (H2O2) induced oxidative stress in Sulfolobus solfataricus (P2) were conducted. Microarray analysis of mRNA expression showed that 102 transcripts …

Long 3’-Utrs Target Wild-Type Mrnas For Nonsense-Mediated Mrna Decay In Saccharomyces Cerevisiae, Bessie W. Kebaara, Audrey L. Atkin

School of Biological Sciences: Faculty Publications

The nonsense-mediated mRNA decay (NMD) pathway,

present in most eukaryotic cells, is a specialized

pathway that leads to the recognition and rapid

degradation of mRNAs with premature termination

codons and, importantly, some wild-type

mRNAs. Earlier studies demonstrated that aberrant

mRNAs with artificially extended 3’-untranslated

regions (3’-UTRs) are degraded by NMD. However,

the extent to which wild-type mRNAs with long

3’-UTRs are degraded by NMD is not known.

We used a global approach to identify wild-type

mRNAs in Saccharomyces cerevisiae that have

longer than expected 3’-UTRs, and of these

mRNAs tested, 91% were degraded by NMD. We

demonstrate for the first …

Role Of Vapbc Toxin–Antitoxin Loci In The Thermal Stress Response Of Sulfolobus Solfataricus, Charlotte R. Cooper, Amanda J. Daugherty, Sabrina Tachdjian, Paul H. Blum, Robert M. Kelly

School of Biological Sciences: Faculty Publications

TA (toxin–antitoxin) loci are ubiquitous in prokaryotic microorganisms, including archaea, yet

their physiological function is largely unknown. For example, preliminary reports have suggested

that TA loci are microbial stress-response elements, although it was recently shown that knocking

out all known chromosomally located TA loci in Escherichia coli did not have an impact on

survival under certain types of stress. The hyperthermophilic crenarchaeon Sulfolobus solfataricus

encodes at least 26 vapBC (where vap is virulence-associated protein) family TA loci in its

genome. VapCs are PIN (PilT N-terminus) domain proteins with putative ribonuclease activity,

while VapBs are proteolytically labile proteins, which purportedly function …

Evolution And Pleiotropy Of Trithorax Function In Arabidopsis, Zoya Avramova

School of Biological Sciences: Faculty Publications

The SET domain-containing genes of the TRITHORAX family encode epigenetic factors that maintain the expression of targeted genes. Trithorax homologs have been found in both animals and plants. Since these are thought to have evolved multicellularity independently, common mechanisms of epigenetic regulation must be evolutionarily ancient and derived from a common ancestor. In addition, each lineage has evolved unique mechanisms to expand the original repertoire of epigenetic functions. Phylogenetic analysis of SET domain proteins has outlined some intriguing evolutionary trends. In plants, epigenetic gene silencing mechanisms have been aggressively pursued. In contrast, studies of epigenetic mechanisms maintaining active gene expression …

Caenorhabditis Elegans Genomic Response To Soil Bacteria Predicts Environment-Specific Genetic Effects On Life History Traits, Joseph D. Coolon, Kenneth L. Jones, Timothy C. Todd, Bryanua C. Carr, Michael A. Herman

School of Biological Sciences: Faculty Publications

With the post-genomic era came a dramatic increase in high-throughput technologies, of which transcriptional profiling by microarrays was one of the most popular. One application of this technology is to identify genes that are differentially expressed in response to different environmental conditions. These experiments are constructed under the assumption that the differentially expressed genes are functionally important in the environment where they are induced. However, whether differential expression is predictive of functional importance has yet to be tested. Here we have addressed this expectation by employing Caenorhabditis elegans as a model for the interaction of native soil nematode taxa and …

The Nuclear Receptor Nhr-25 Cooperates With The Wnt/Β-Catenin Asymmetry Pathway To Control Differentiation Of The T Seam Cell In C. Elegans, Martina Hajduskova, Marek Jindra, Michael A. Herman, Masako Asahina

School of Biological Sciences: Faculty Publications

Asymmetric cell divisions produce new cell types during animal development. Studies in Caenorhabditis elegans have identified major signal-transduction pathways that determine the polarity of cell divisions. How these relatively few conserved pathways interact and what modulates them to ensure the diversity of multiple tissue types is an open question. The Wnt/β-catenin asymmetry pathway governs polarity of the epidermal T seam cell in the C. elegans tail. Here, we show that the asymmetry of T-seam-cell division and morphogenesis of the male sensory rays require NHR-25, an evolutionarily conserved nuclear receptor. NHR-25 ensures the neural fate of the T-seam-cell descendants in cooperation …

The Impact Of Co-Occurring Tree And Grassland Species On Carbon Sequestration And Potential Biofuel Production, Ramesh Laungani, Johannes Knops

School of Biological Sciences: Faculty Publications

We evaluated how three co-occurring tree and four grassland species influence potentially harvestable biofuel stocks and above- and belowground carbon pools. After 5 years, the tree Pinus strobus had 6.5 times the amount of aboveground harvestable biomass as another tree Quercus ellipsoidalis and 10 times that of the grassland species. P. strobus accrued the largest total plant carbon pool (1375 gCm -2 or 394 gCm -2 yr), while Schizachyrium scoparium accrued the largest total plant carbon pool among the grassland species (421 gCm -2 or 137 gCm -2 yr). Quercus ellipsoidalis accrued 850 gCm -2, Q. macrocarpa 370 gCm -2, …

Crystallization And Crystal-Packing Studies Of Chlorella Virus Deoxyuridine Triphosphatase, Kohei Homma, Hideaki Moriyama

School of Biological Sciences: Faculty Publications

The 141-amino-acid deoxyuridine triphosphatase (dUTPase) from the algal Chlorella virus IL-3A and its Glu81Ser/Thr84Arg-mutant derivative Mu-22 were crystallized using the hanging-drop vapor-diffusion method at 298 K with polyethylene glycol as the precipitant. An apo IL-3A dUTPase with an aminoterminal T7 epitope tag and a carboxy-terminal histidine tag yielded cubic P213 crystals with unit-cell parameter a = 106.65 A . In the presence of dUDP, the enzyme produced thin stacked orthorhombic P222 crystals with unit-cell parameters a = 81.0, b = 96.2, c = 132.8 A. T7-histidine-tagged Mu-22 dUTPase formed thin stacked rectangular crystals. Amino-terminal histidine-tagged dUTPases did not crystallize but …

Ecological Genomics Of Nematode Community Interactions: Model And Non-Model Approaches, Michael A. Herman, Joseph D. Coolon, Kenneth L. Jones, Timothy Todd

School of Biological Sciences: Faculty Publications

The effects of human-induced environmental change are evident at multiple levels of biological organization. To date, most environmental change studies have focused on effects at the ecosystem, community, and organismal levels. However, the ultimate controls of biological responses are located in the genome. Thus, genetic and genomic studies of organismal responses to environmental changes are necessary. Recent advances in genome analysis now make such analyses possible. In this chapter we describe a research approach and program that can begin to span this gap by using genome-enabled approaches to characterize organismal changes and then employing a genetically tractable model organism to …

A Study Of The Waterborne And Dietary Toxicityof Cadmium To Hydra Attenuata And Daphnia Plux In Soft Waters And The Development Of Biotic Ligand Models To Predict Such Toxicity, Matthew S.A. Clifford

Theses and Dissertations (Comprehensive)

The goal of this thesis was to compare the effects of waterborne Cd exposures and dietary Cd exposures to Hydra attenuata. Before the Hydra could be exposed to dietborne exposures of Cd, the effects of waterborne exposures had to be understood. This was done by looking at the sensitivity of D. pulex to Cd and then understanding the accumulation of Cd. After bioaccumulation patterns of Cd. to D. pulex were known, we could then feed the prey to the Hydra and comapre the effects to waterborne effects of Cd to Hydra.

To understand the waterborne effects of Cd to …

Using Chemical, Optical And Biological Methods To Characterize Cu Complexation By Different Natural Organic Matter Sources Collected From Canadian Shield Waters, Keegan Andrew Hicks

Theses and Dissertations (Comprehensive)

The effects of natural organic matter (NOM) source on Cu complexation were characterized chemically, optically and biologically in soft waters. NOM from different Canadian Shield soft water lakes were concentrated using reverse osmosis and for comparison NOM was also sampled from a hard water source. NOM complexation capacity was assessed directly in Cu spiked solutions (with and without additions of NOM) by measuring free Cu activity using an ion selective electrode. Additional chemical and optical characterizations of NOM included specific absorbance coefficient (SAC; at 340 nm), excitation-emission matrix spectroscopy, protein content, and molecular weight fractionation (fraction of DOCkDa). The biological …

Metabolic And Molecular Approaches To The Study Of Bacterial Communities In Wetlands Of The Alberta Athabasca Oil Sands Region, Jessica Dawn Morrison

Theses and Dissertations (Comprehensive)

Bacterial communities in wetlands from the Athabasca region (Alberta, Canada) were surveyed and their utility as bio-indicators for wetland reclamation was assessed. Sediment samples were collected from wetlands categorized as: (1) natural (off mining leases), (2) reference (on mining sites but not directly impacted by oil sands processed material (OSPM)), and (3) OSPM (directly affected by OSPM). Wetlands of the latter two groups ranged in age from 11 to 24 years. Analysis involved community level physiological profiling (CLPP) with BIOLOG™ EcoPlates, and denaturing gradient gel electrophoresis (DGGE). Multivariate analysis applied to data from both techniques revealed differences in the bacterial …

Characterization Of The Interaction Between Arabidopsis Toc159 And Toc33 Gtpase Domains, Wesley A. Farquharson

Theses and Dissertations (Comprehensive)

Protein-protein interactions are critical for many biochemical processes and for cellular function. Understanding interactions between proteins and ligands, as well as other proteins are necessary for the modelling of biological systems. Protein import into chloroplasts is one such system where the understanding of interactions between members of the translocation complex is limited. The translocon at the outer envelope membrane of chloroplasts (Toe complex) consists of three core components: Toc75, Tocl59, and Toc34. Members of the Tocl59 and Toc34 gene families in Arabidopsis thaliana have been shown to assemble differentially into structurally and functionally distinct complexes. More specifically, there is evidence …

The Epidemiological Status Of Pav1, And The Effects Of Infection On Caribbean Spiny Lobster (Panulirus Argus) Condition, Olfaction, And Predation Risk, Donald C. Behringer, Mark J. Butler Iv, Jeffrey D. Shields

Biological Sciences Faculty Publications

No abstract provided.

The Costs Of Transportation At Mckillop Library, Elizabeth Giambusso, Timothy Hanrahan, Raine Raab

BIO140 Carbon Footprint Project

Much like other libraries and institutions across the United States, Salve Regina faces its share of efficiency problems. In turn, these problems add to the many environmental issues we face today. These are mostly paper waste, recycling, or lack thereof, and the amount of energy needed to heat buildings. Notwithstanding the excessive consumption and waste, our group focused on transportation. The main goal of this project was to measure the current transportation costs and explore ways to cut carbon emissions. By sending a questionnaire through email, the staff responded with the type of vehicle they drive and their commuting distance. …

Student Assessment Of The Environmental Footprint Of The Mckillop Library, Salve Regina University - Executive Summary, Jameson Chace

BIO140 Carbon Footprint Project

No abstract provided.

Paper Waste In Mckillop Library, Kristina Lowe, Charles "Ben" Parrish, William Pearce, Sarah Petro, Allison Smith, Sarah Tolman

BIO140 Carbon Footprint Project

The goals of our project were to determine the amount of the library’s paper waste. In order to do this we decided to focus on the total sheets of paper used, the recycling that occurs in the library, the use of recycled paper, and the discarding of books. The methods that were used were talking to library staff about the amount of paper used and the discarding of books, surveying the students who use the library about their recycling practices, and hanging educational flyers about the benefits of recycling. After researching the amount of paper waste in McKillop Library, the …

Green Heating And The Mckillop Library Building, Alexandra Riewer, Anthony Rothwell, Kalei Sullivan, Other Student

BIO140 Carbon Footprint Project

As global warming becomes an ever increasing problem, it is Salve Regina University and the Newport communities job to do their part. In an effort to reduce their carbon footprint, we performed a series of tests on the heating and building of McKillop library at Salve Regina to measure its carbon emissions and environmental impact. As a team, we tested for areas of heat loss and temperature change and internal temperature and humidity fluctuation. We also focused on the types of insulation that the building uses and the efficiency of its HVAC – heating, ventilation, and air conditioning – system. …

Appendices

BIO140 Carbon Footprint Project

No abstract provided.

Dynamics Of Aggregation Formation In Japanese Beetles, Popillia Japonica, Katelyn A. Kowles

Masters Theses

For most insect aggregations to form, they need to be started by an initial individual (the pioneer) andjoined by later individuals (the joiners). Pioneers and joiners may differ with regard to characteristics such as sex and body size. We carried out three field experiments to examine the characteristics of Japanese beetles, Popillia japonica, pioneering and joining aggregations on host plants. Individual beetles were captured as they arrived on uninhabited grape plants, as well as plants designed to simulate aggregations with model beetles and feeding damage. For all experiments and treatments, the beetles arriving were significantly female-biased, with pioneer females having …

Ecology And Impacts Of Lianas In Regenerating Forests, Laura Marie Ladwig

Masters Theses

Although they are important components of forest communities, the general ecology of temperate lianas, especially during forest regeneration, is largely unknown. The dependence of lianas on other plants for physical support makes them a potentially important driver of community dynamics. I examined current and past vegetation data from an old-field succession study in the Piedmont region of New Jersey, USA to determine the dynamics of liana expansion and their role in forest regeneration. Fifty years of vegetation data were utilized to determine community controls and population dynamics oflianas during expansion. Five lianas, Celastrus orbiculatus, Lonicera japonica, Parthenocissus quinquefolia, Toxicodendron radicans, …

Aging In The Mammalian Olfactory System, Stephen Clark

Masters Theses

The purpose of this investigation was to investigate genetic and age~related differences in the mammalian olfactory system. The molecule of interest is apolipoprotein E (apoE), a component oflipoprotein molecules which functions in the transport of cell membrane components (triglycerides, lipids, and cholesterol). Specifically in the nervous system, apoE is synthesized by glial cells that function in recycling dead nervous tissue components for delivery to growing or dividing neurons. ApoE is present in three protein isofon11s: apoE2, which has been associated with type III hyperlipoproteinemia; apoE3, rhe normal human isoforrn; and apoE4, which has been associated with Alzheimer's disease (AD). The …

Interaction Of Apoe With Estrogen In The Olfactory System During Nerve Maintenance And Recovery, Michael M. Tonsor Ii

Masters Theses

Estrogen replacement profoundly aids in nerve regeneration after injury in adult mammalian models. Estrogen replacement also protects against olfactory dysfunction in mammals. The mechanism underly1ng estrogen's neuroprotective effects remains ambiguous. My goal is to investigate and better clarify if apolipoprotein E (apoE ), a lipid transporting protein, is a critical intermediary to estrogen's effects on nerve maintenance and regeneration.

My hypothesis is that apoE is a mediator of estrogen's beneficial effects. This hypothesis is based on the following observations from previous studies. First, olfactory nerve regeneration was delayed in apoE-gene knockout (KO) mice as compared to wild type (WT) mice. …

Fragmentation Effects On Fitness In Five Common Prairie Species, Lydia Miramontes Loyd

Masters Theses

Midwest tallgrass prairies have suffered extensive habitat loss and as a result persist as isolated and highly fragmented remnants. In such small, isolated remnants, genetic drift and inbreeding depression can lead to a loss of genetic variation over time. Reduced genetic variation is often associated with reduced levels of growth, survival, and reproduction as well as increased susceptibility to stress. This study examined fitness traits of five common prairie species to determine whether plants in small fragmented remnants had reduced fitness as compared to those in a larger remnant. Five prairie species were chosen for this study based on their …

Nest Success, Nesting Habitat, & Nestling Feeding Rates Of Red-Headed Woodpeckers In East-Central Illinois, Nathan Cole Hudson

Masters Theses

The Red-headed Woodpecker (Melanerpes erythrocephalus) is a classic oak savanna species that has suffered significant population declines caused, in part, by extensive loss of savanna habitat and agricultural development. Despite consistent data that reveal a dramatic decline throughout much of their range, few studies have examined other possible explanations. In an effort to determine potential causes for their continued decline (as well as add to existing knowledge of nesting biology), we examined nest success and nesting micro-habitat features. We compared micro-habitat variables at: 1) golf and non-golf course sites, 2) successful and failed nest sites, and 3) nest and random …

Bibliography Of The Frogs Of The Leptodactylus Clade – Adenomera, Hydrolaetare, Leptodactylus, Lithodytes (Amphibia, Anura, Leptodactylidae) Volume 2. Indices, Miriam H. Heyer, W. Ronald Heyer, Rafael O. De Sá

Biology Faculty Publications

The bibliography (Volume 1) is organized alphabetically by last name of the first author. The Species Index, Keyword Index, and Geographic Index are organized by the unique number that EndNote® assigns to each record. To find a reference from the three indices, one must utilize the References by EndNote® Number, listed in numerical order. There is sufficient information associated with the EndNote® numerical order entry to find the specific reference involved in Volume 1 – References.

Complete authors names are used in the EndNote® Number index for the EndNote® records that contain such information. Note that there are some “missing” …