Digital Commons Network^™

An Examination Of The Correlation Between Shoot Apical Meristem Size And Leaf Heterophylly In Pisum Sativum, Cynthia Mary Halfman

Undergraduate Honors Thesis Collection

This study is a quantitative examination of the ramifications of leaf development as influenced by the size of the shoot apical meristem. I wish not to delve into a genetic examination of the shoot apical meristem but rather into the possible correlation between the apical meristem size and leaf heterophylly. If changes in shoot apical meristem size influence heterophylly in leaves, then as shoot apical meristem changes, leaf characteristics will change. This change may result from two different relationships.

Apicomplexan Parasites Co-Opt Host Calpains To Facilitate Their Escape From Infected Cells, Rajesh Chandramohanadas, Paul H. Davis, Daniel P. Beiting, Michael B. Harbut, Claire Darling, Geetha Velmourougane, Ming Yeh Lee, Peter A. Greer, David S. Roos, Doron C. Greenbaum

Biology Faculty Publications

Apicomplexan parasites, including Plasmodium falciparum and Toxoplasma gondii (the causative agents of malaria and toxoplasmosis, respectively), are responsible for considerable morbidity and mortality worldwide. These pathogenic protozoa replicate within an intracellular vacuole inside of infected host cells, from which they must escape to initiate a new lytic cycle. By integrating cell biological, pharmacological, and genetic approaches, we provide evidence that both Plasmodium and Toxoplasma hijack host cell calpain proteases to facilitate parasite egress. Immunodepletion or inhibition of calpain-1 in hypotonically lysed and resealed erythrocytes prevented the escape of P. falciparum parasites, which was restored by adding purified calpain-1. Similarly, efficient …

Analysis And Classification Of Sounds Produced By Asian Elephants (Elephas Maximus), Sharon Stuart Glaeser

Dissertations and Theses

Relatively little is known about the vocal repertoire of Asian elephants (Elephas maximus), and a categorization of basic call types and modifications of these call types by quantitative acoustic parameters is needed to examine acoustic variability within and among call types, to examine individuality, to determine communicative function of calls via playback, to compare species and populations, and to develop rigorous call recognition algorithms for monitoring populations.

This study defines an acoustic repertoire of Asian elephants based on acoustic parameters, compares repertoire usage among groups and individuals, and validates structural distinction among call types through comparison of manual and automated …

Isolation And Characterization Of A Spontaneously Immortalized Bovine Retinal Pigmented Epithelial Cell Line, Thomas E. Liggett, T. Daniel Griffiths, Elizabeth R. Gaillard

Biological Sciences Faculty Research and Publications

Background

The Retinal Pigmented Epithelium (RPE) is juxtaposed with the photoreceptor outer segments of the eye. The proximity of the photoreceptor cells is a prerequisite for their survival, as they depend on the RPE to remove the outer segments and are also influenced by RPE cell paracrine factors. RPE cell death can cause a progressive loss of photoreceptor function, which can diminish vision and, over time, blindness ensues. Degeneration of the retina has been shown to induce a variety of retinopathies, such as Stargardt's disease, Cone-Rod Dystrophy (CRD), Retinitis Pigmentosa (RP), Fundus Flavimaculatus (FFM), Best's disease and Age-related Macular Degeneration …

The Rise Of The Classical Gene: A Review Of In Pursuit Of The Gene: From Darwin To Dna, Michael Dietrich

Dartmouth Scholarship

Review of The rise of the classical gene: a review of In Pursuit of the Gene: From Darwin to DNA by James Schwartz, 2008.

Comparative Vegetative Anatomy And Systematics Of Laeliinae (Orchidaceae), William Stern, Barbara Carlsward

Faculty Research & Creative Activity

Laeliinae are one of the most prominent orchid subtribes, with c. 40 genera and nearly 1500 species, and contain a disparate group of taxa with widely varying morphological features. There does not appear to be a complex of characters to which one can refer in order to delineate the subtribe as a whole. Thus, it was thought that vegetative anatomy might provide clues to the monophyly of the group. The microscopic structure of the leaves, stems and roots of representatives of most of the genera was studied. It was concluded that the anatomy lacks overall uniformity and that vegetative characters …

Comparative Vegetative Anatomy And Systematics Of Laeliinae (Orchidaceae), William Louis Stern, Barbara S. Carlsward

Faculty Research & Creative Activity

Laeliinae are one of the most prominent orchid subtribes, with c. 40 genera and nearly 1500 species, and contain a disparate group of taxa with widely varying morphological features. There does not appear to be a complex of characters to which one can refer in order to delineate the subtribe as a whole. Thus, it was thought that vegetative anatomy might provide clues to the monophyly of the group. The microscopic structure of the leaves, stems and roots of representatives of most of the genera was studied. It was concluded that the anatomy lacks overall uniformity and that vegetative characters …

Thyroid Hormone Regulation Of Mrnas Encoding Thyrotropin Β-Subunit, Glycoprotein Α-Subunit, And Thyroid Hormone Receptors Α And Β In Brain, Pituitary Gland, Liver, And Gonads Of An Adult Teleost, Pimephales Promelas, Sean C. Lema, Jon T. Dickey, Irvin R. Schultz, Penny Swanson

Biological Sciences

Thyroid hormones (THs) regulate growth, morphological development, and migratory behaviors in teleost fish, yet little is known about the transcriptional dynamics of gene targets for THs in these taxa. Here, we characterized TH regulation of mRNAs encoding thyrotropin subunits and thyroid hormone receptors (TRs) in an adult teleost fish model, the fathead minnow (Pimephales promelas). Breeding pairs of adult minnows were fed diets containing 3,5, 3^,-triiodo-_L-thyronine (T₃) or the goitrogen methimazole for 10 days. In males and females, dietary intake of exogenous T₃ elevated circulating total T₃, while methimazole …

A Comparative Ecological Study Of Two Sister Species Of Darters In Kentucky, Etheostoma Kantuckeense And Etheostoma Lawrencei, Bjorn Victor Schmidt

Masters Theses & Specialist Projects

Etheostoma kantuckeense and E. lawrencei are former members of the wide ranging E. spectabile species complex. Etheostoma kantuckeense is endemic to the Barren River Basin in Southern Kentucky and Northern Tennessee, while E. lawrencei occurs in the Green River, Salt River, and Cumberland River Basins of Central and Eastern Kentucky. Isolation of populations within these drainages has allowed for a relatively recent evolutionary divergence, leading to slight differences in morphology. This study was conducted to address if geographical isolation has led to measurable differences in the ecology of these two species. In particular, habitat preference across three spatial scales and …

The Effect Of Breeding Herd Parity Structure On Genetic Improvement Of The Sow Herd, Caitlyn E. Abell

Mahurin Honors College Capstone Experience/Thesis Projects

This study focuses on the value of the genetic lag associated with maintaining sows for additional parities in a commercial swine herd. Three traits were included in this study: number born alive (NBA), 21 day litter weight (W21), and days to market (D250). The economic values assigned to these traits were $22.00/pig, $0.70/lb., and $0.17/day, respectively. The genetic improvement per generation made for each trait was assumed to be 0.3 pigs, 3.0 lbs., and 3.0 days, respectively. It was estimated that the value of the genetic lag associated with retaining a sow to P3, P5, and P7 was $24.80, $46.89, …

Empirical Testing Of 16s Rrna Gene Pcr Primer Pairs Reveals Variance In Target Specificity And Efficacy Not Suggested By In Silico Analysis, Sergio E. Morales, William E. Holben

Biological Sciences Faculty Publications

Phylogenetic and "fingerprinting" analyses of the 16S rRNA genes of prokaryotes have been a mainstay of microbial ecology during the last two decades. However, many methods and results from studies that rely on the 16S rRNA gene for detection and quantification of specific microbial taxa have seemingly received only cursory or even no validation. To directly examine the efficacy and specificity of 16S rRNA gene-based primers for phylum-, class-, and operational taxonomic unit-specific target amplification in quantitative PCR, we created a collection of primers based solely on an extensive soil bacterial 16S rRNA gene clone library containing similar to 5,000 …

Intersubunit Interactions Modulate Ph-Induced Activation Of Membrane Fusion By The Junín Virus Envelope Glycoprotein Gpc, Joanne York, Jack H. Nunberg

Biological Sciences Faculty Publications

The mature arenavirus envelope glycoprotein GPC is a tripartite complex comprising a stable signal peptide (SSP) in addition to the receptor-binding (G1) and transmembrane fusion (G2) subunits. We have shown previously that SSP is a key element in GPC-mediated membrane fusion, and that GPC sensitivity to acidic pH is modulated in part through the lysine residue at position 33 in the ectodomain loop of SSP (J. York and J. H. Nunberg, J. Virol. 80: 7775-7780, 2006). A glutamine substitution at this position stabilizes the native GPC complex and thereby prevents the induction of pH-dependent membrane fusion. In efforts to identify …

Common Garden Comparisons Of Native And Introduced Plant Populations: Latitudinal Clines Can Obscure Evolutionary Inferences, Robert I. Colautti, John L. Maron, Spencer C.H. Barrett

Biological Sciences Faculty Publications

Common garden studies are increasingly used to identify differences in phenotypic traits between native and introduced genotypes, often ignoring sources of among-population variation within each range. We re-analyzed data from 32 common garden studies of 28 plant species that tested for rapid evolution associated with biological invasion. Our goals were: (i) to identify patterns of phenotypic trait variation among populations within native and introduced ranges, and (ii) to explore the consequences of this variation for how differences between the ranges are interpreted. We combined life history and physiologic traits into a single principal component (PC_ALL) and also compared …

Do Hypothermic Tissue Tolerances Limit Torpor Expression?, Clark J. Cotton, Henry J. Harlow

Biology Faculty Publications

1. Arrest temperatures and Q₁₀ values for extensor digitorum longus (EDL), soleus, trabecula, and jejunum muscle twitch strength, contraction time, and 0.5 relaxation time were calculated for a deep torpor hibernator, white-tailed prairie dog (WTPD) (Cynomys leucurus), a shallow torpor hibernator, black-tailed prairie dog (BTPD) (Cynomys ludovicianus), and a non-hibernator, lab rat (Rattus norvegicus) to test the hypothesis that tissue temperature tolerances limit the depth of expressed torpor.

2. There were no temperature tolerance differences between the tissues of the two species of hibernators. Both hibernating species had arrest temperatures and Q_{10 …}

The Four-And-A-Half Lim Domain Protein 2 Regulates Vascular Smooth Muscle Phenotype And Vascular Tone, Nicole A. Neuman, Susan Ma, Gavin R. Schnitzler, Yan Zhu, Giorgio Lagna, Akiko Hata

Biology

In responsetovascular injury, differentiated vascular smooth muscle cells (vSMCs) undergo a uniqueprocess knownas“phenotype modulation,” transitioning from a quiescent, “contractile” phenotype to a proliferative, “synthetic” state. We have demonstrated previously that the signaling pathway of bone morphogenetic proteins, members of the transforming growth factor family, play a role in the induction and maintenance of a contractile phenotype in human primary pulmonary artery smooth muscle cells. In this study, we show that a four-and-ahalf LIM domainprotein 2(FHL2)inhibits transcriptional activation ofvSMC-specificgenesmediatedbythebonemorphogenetic protein signaling pathway through the CArG box-binding proteins, such as serum response factor and members of the myocardin (Myocd) family. Interestingly, FHL2 …

The Effects Of Stream Discharge And Channelization On The Macroinvertebrate Community In A Semi-Arid Landscape, Thomas L. Kennedy

Biology ETDs

The Middle Rio Grande is the second largest watershed in the southwestern United States and is a vitally important component for the biodiversity in the region. Similar to many other rivers, the MRG is a temporally dynamic system that unfortunately, has been subjected to river regulation. As a result, the river has become channelised in many reaches disconnecting it from the surrounding floodplain. The effects of river regulation on the MRG and its surrounding riparian zone were largely unknown. I tested three main hypothesis with regards to the macroinvertebrates in the MRG: 1) Does flow variability structure the aquatic macroinvertebrate …

Controls On Microbial Community Structure In Thermal Environments: Exploring Bacterial Diversity And The Relative Influence Of Geochemistry And Geography, Kendra Renee Mitchell

Biology ETDs

Community wide molecular surveys have revealed incredible hidden phylogenetic and metabolic diversity in microbial habitats. We have conducted the first microbial survey of Yellowstone National Park thermal environments, sampling 103 communities from across the park and across the range of conditions found. Yellowstone is particularly suited for this type of research because of the large number and wide variety of thermal springs, which are naturally occurring chemostats enabling examination of the factors that control microbes and drive community structure. In addition to samples for molecular microbial analysis, we collected water for extensive geochemical analysis in order to begin to deduce …

Hydration Changes Of Dna Binding By Klentaq And Klenow Measured By Osmotic Stress, John Tod Baker

Honors Capstones

No abstract provided.

Oxidative Stress Induced Hu Protein Expression In Deinococcus Radiodurans, Cara Bevinetto

Honors Capstones

No abstract provided.

Characterization Of The Catalytic Mechanism Of The Carboxyltransferase Component Of Escherichia Coli Acetyl-Coa Carboxylase Leading To Rational Inhibitor Identification And The Development Of Enzymatic Mimics, Nabil K. Thalji

Honors Capstones

No abstract provided.

Rescuing Minute-Like Phenotypes Due To Rnai-Mediated Knockdown Of Nopp140 By Deletion Of P53 In Drosophila Melanogaster, Renford Cindass Jr

Honors Capstones

No abstract provided.

Cytokine Production During Microsporidia Infection Of Macrophages, Anne Louise Hotard

Honors Capstones

No abstract provided.

Cytokine Production During Microsporidia Infection Of Macrophages, Anne Hotard

Honors Capstones

No abstract provided.

Conservation Genetics Of Boelen‟S Python (Morelia Boeleni) From New Guinea: Reduced Genetic Diversity And Divergence Of Captive And Wild Animals, Joshua Lee Jordan

Honors Capstones

No abstract provided.

Determination Of Dispersal Patterns Of The Small-Mouthed Salamander (Ambystoma Texanum) In Eagle Creek Park (Indianapolis, In), Stacey Diane Summitt

Undergraduate Honors Thesis Collection

Urbanization and the increasing threat of habitat fragmentation are contributing to the significant declines in amphibian populations world-wide. Ambystomatid species are particularly susceptible to habitat fragmentation because they migrate yearly across upland forests to their breeding sites. Habitat changes could be altering the genetic structure in these species; however few studies have focused on this topic and more generally, examined the dispersal patterns of Ambystomatids. In 2003, Clark, Cripe, and Stachniw conducted a study on “Metapopulation Structure of Ambystoma texanum in Eagle Creek Park (Indianapolis, IN) and the Potential for Gene Flow.” They hypothesized that distance limits the dispersal of …

Binding Of Icer To Its Own Promoter As A Mode Of Cooperative Regulation, Craig Stephen Queenan

Theses, Dissertations and Culminating Projects

Inducible cAMP Early Repressor (ICER) is a regulator of cAMP signaling in the cell. ICER can be found in normal cells, but it is not present in tumor cells. The growth of these tumor cells is hampered when ICER protein is artificially reintroduced in these cells. Therefore, it is hypothesized that ICER manipulation could potentially be used as a new therapy for the treatment of cancer. Although much is known about the physiological role of ICER, little is known about the regulation of the gene. In this study, the negative autoregulation of ICER by binding to its own regulatory sequences, …

Down Regulation Patterns Of The Mutated Ret Receptor Tyrosine Kinase, Mai A. Soliman

Theses, Dissertations and Culminating Projects

REarranged during Transfection (RET) is a mammalian tyrosine kinase receptor involved in the signal transduction pathways during early embryonic kidney and enteric nervous system development. Activation of RET is mediated by one of the membrane associated co-receptors GFRal-4 and one of the four GFL family ligands. Following ligand binding, RET undergoes autophosphorylation followed by downstream activation of additional cell signaling proteins. However, less is understood about the down- regulation patterns of RET and of naturally occurring RET mutants and laboratory modified forms of RET. In this study, the role of the cytoplasmic domain in receptor processing and protein degradation was …

Environmental Regulation Of Dormancy Loss In Lomatium Dissectum (Apiaceae) Seeds, Melissa Scholten, Jacklyn Donahue, Nancy L. Shaw, Marcelo Serpe

Biology Faculty Publications and Presentations

Background and aims Lomatium dissectum (Apiaceae) is a perennial, herbaceous plant of wide distribution in Western North America. At the time of dispersal, L. dissectum seeds are dormant and have underdeveloped embryos. The aims of this work were to determine the requirements for dormancy break and germination, to characterize the type of seed dormancy, and to determine the effect of dehydration after embryo growth on seed viability and secondary dormancy.

Methods The temperature requirements for embryo growth and germination were investigated under growth chamber and field conditions. The effect of GA3 on embryo growth was also analyzed to determine the …

Imaging Of Rnase Mitochondrial Rna Processing Localization Using Fluorescent Microscopy, Bryan Marascalchi

Renée Crown University Honors Thesis Projects - All

This paper presents a method for imaging RNase mitochondrial RNA processing localization using fluorescent microscopy. RNase mitochondrial RNA Processing, MRP, is an essential ribonucleoprotein endoribonuclease that functions in the degradation of specific mRNAs that control the cell cycle. A mutation of the RNA component of RNase MRP leads to a genetic disorder called Cartilage-Hair Hypoplasia. This disorder results from the decreased rate of cell growth because the mutant cell no longer has the ability to assemble rRNA, and cyclin-dependent cell-cycle regulation no longer functions correctly. My Capstone Project will study the localization and regulation of RNase MRP so that we …

Evolution Of Craniofacial Architecture And Lateralization In Perissodini Scale-Eaters, Thomas A. Stewart

Renée Crown University Honors Thesis Projects - All

To understand the evolution of craniofacial asymmetry within the Perissodini tribe, a group of scale-eating fish found in Lake Tanganyika, we performed shape analysis on their craniofacial architecture, and applied a simple lever model to measure asymmetry in lower-jaw mechanics. We found that craniofacial asymmetries were the consequence of sided differences in size and shape of craniofacial elements, and that sided differences in shape predict the lateralization of force and speed of the lower jaw in derived species. Quantitative Trait Locus analysis in Lake Malawi cichlids identified a single locus of major effect for jaw laterality, the first genetic marker …