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Altered Calcium Handling In Cerebellar Purkinje Neurons With The Malignant Hyperthermia Mutation, Ryr1-Y522s/+, George C. Talbott

Electronic Theses and Dissertations

To investigate the etiology of malignant hyperthermia and central core disease, mouse models have recently been generated and characterized (Chelu et al., 2006). These RyRY522S/+ knock-in mutant mice provide an excellent tool to investigate calcium dysregulation, its pathological consequences, and potential therapeutic approaches. Skeletal muscle harboring this mutation exhibits calcium leak from internal stores and an increased sensitivity to activation by caffeine, voltage, and temperature (Durham et al., 2008). Although alterations in RyR1 channel function and resultant changes in cellular function have been characterized in skeletal muscle, the effects of MH mutations in RyR1 on central nervous system function have …

The Vocal Behaviors Of North American River Otters (Lontra Canadensis) Individual Differences And Shared Repertoires, Carla Almonte

Dissertations, Theses, and Capstone Projects

The current information on the vocal repertoire of the North American River Otter is very limited. To date there have been no direct studies conducted on their repertories. In this study, I examined the vocal behavior of 12 captive river otters. The discriminant function analysis suggests that river otters have 4 distinct call types with 7 sub-call types and one call the whistle is unique to one group of pups. The results of the Kruskal-Wallis comparing acoustical structures shows strong evidence for the presence of individuality with some individuals showing greater differences in comparison to the others. I also examined …

Interactions Of Diet And Behavior In A Death-Feigning Snake (Heterodon Nasicus), Andrew Michael Durso

Masters Theses

Studies of animal behavior in captivity are limited in their ability to explain the influence of a natural environment on behavioral ecology. Defensive behaviors vary among individual animals, between sexes and with age, as well as with other less well-known factors. The toxin-rich diet of many toad-eating snakes might enable or cause their passive terminal defensive behavior of death-feigning. I videotaped death-feigning behavior in wild Plains Hog-Nosed Snakes (Heterodon nasicus) subjected to standardized harassment. Using stable isotopes, I also described the diet of the same individuals subjected to behavioral analysis (n = 40). Heterodon had broader diets than other snakes …

Symbionts As An Epigenetic Source Of Heritable Variation, Scott F. Gilbert

Biology Faculty Works

No abstract provided.

Evolutionary Developmental Biology And Intelligent Design, Scott F. Gilbert

Biology Faculty Works

No abstract provided.

Impact Of Land Management On House Mice And Red Imported Fire Ants, Jesse R. Abelson

Electronic Theses and Dissertations

Understanding of mechanisms that limit the abundance and distribution of species is central to ecology. The failure of mechanisms to regulate populations can result in population outbreaks. There have been two outbreaks of house mice in the past decade in central Florida. In my study, I examine the efficacy of landscape management in the form of mowing and plowed soil barriers to limit or prevent outbreaks of house mice in a former agricultural area. House mouse populations were highly variable, but were unaffected by mowing or plowed soil barriers. Red imported fire ants were ubiquitous in the study area regardless …

Reinventing Richard Goldschmidt: Reputation, Memory, And Biography, Michael Dietrich

Dartmouth Scholarship

Richard Goldschmidt was one of the most controversial biologists of the mid-twentieth century. Rather than fade from view, Goldschmidts work and reputation has persisted in the biological community long after he has. Goldschmidts longevity is due in large part to how he was represented by Stephen J. Gould. When viewed from the perspective of the biographer, Goulds revival of Goldschmidt as an evolutionary heretic in the 1970s and 1980s represents a selective reinvention of Goldschmidt that provides a contrast to other kinds of biographical commemorations by scientists.\r\n\r\nKeywords: Richard Goldschmidt, hopeful monsters, evolutionary synthesis, Stephen J. Gould, punctuated equilibrium, biography

Modeling Carbon Allocation, Growth And Recovery In Scrub Oaks Experiencing Aboveground Disturbance, Troy J. Seiler

Electronic Theses and Dissertations

Allocation of assimilated carbon amongst plant metabolic processes and tissues is important to understanding ecosystem carbon cycles. Due to the range of spatio-temporal scales and complex process interactions involved, direct measurements of allocation in natural environments are logistically difficult. Modeling approaches provide tools to examine these patterns by integrating finer scale process measurements. One such method is root:shoot balance, where plant growth is limited by either shoot activity (i.e. photosynthesis) or root activity (i.e. water and nutrient uptake). This method shows promise for application on frequently disturbed systems which perturb aboveground biomass and thus create imbalances in root and shoot …

The Effects Of Age On Reproduction In A Citrus Root Weevil Diaprepes Abbreviatus, Heather R. Chasez

Electronic Theses and Dissertations

Understanding the factors influencing mate choice is a major focus of sexual selection. Many factors are potentially involved, including age of the individual. The good genes model and the youth advantage model both make predictions about the effect of age on mate choice. Under the good genes model older mates would be the more preferable due to their proven high survivability. The “youth advantage” model, predicts that young to intermediate age males would be more advantageous as mates because of a decrease in sperm quality and the possibility of increased germ-line mutations in older animals. I examined the effects of …

Understanding The Invasion Of Florida's Intertidal Crassostrea Virginica Reefs By Non-Native Marine Invertebrate Species, Ethan Fletcher Nash

Electronic Theses and Dissertations

Predicting the locations of new biological invasions has become a high priority for biologists as well as trying to predict if newly introduced species will become damaging to native ecosystems. Reefs of the eastern oyster Crassostrea virginica in Mosquito Lagoon, Florida have been highly disturbed in recent years resulting in dead reefs (piles of dead, disarticulated shells) some of which have been restored. I conducted oyster reef surveys for non-native invertebrates to determine if disturbance on these oyster reefs might assist invasion by two species, Mytella charruana and Perna viridis, recently introduced to the southeastern coast of the United States. …

Preliminary Report: Investigation Of Castor Bean Investment In Extra-Floral Nectary Glands And Reproduction, Victor D. Carmona-Galindo, Kavita Goss, Tina Marie Moger, Ashton Nielson

Biology Faculty Works

The castor bean plant, Ricinus communis has EFN (extrafloral nectary) glands that attract ants which provide defense for the plant. From this, we proposed the idea that the more EFN glands a plant has, the more ants will visit the plant, and therefore provide more defense to the plant. Using the principle of allocation, we deduced that a plant using this strategy would invest less energy in reproduction than in defense, given that growth is fixed. In this experiment we examined the number of reproductive flowers that a plant produces (male and female) in relation to the number of EFN …

Relationship Between Soil Sodium Concentration And Plant Height In Salicornia Virginica In The Ballona Wetlands In Los Angeles, California, Victor D. Carmona, Molly Bogeberg, Stephanie Vivanco

Biology Faculty Works

Previous studies have shown a positive relationship between biomass of the common wetland halophyte, Salicornia virginica, and salinity. This study implements a new technique that is less invasive than measuring total plant biomass that involves uprooting the plant. Instead, plant height was used as an indicator of sodium content in surrounding soils. This technique is based on the hypothesis that plant height of S. virginica corresponds to soil sodium concentrations. There was a significant variation in plant height at higher soil sodium concentrations than at lower soil sodium concentrations. Furthermore, in areas of higher soil water content (%), sodium concentration …

A Preliminary Study Of Heteromeles Arbutifolia Fruit Morphology At Ballona Wetlands And Temescal Canyon, Los Angeles, California, Victor D. Carmona, Phil Simmons, Elise Vo, Elizabeth Wight

Biology Faculty Works

Heteromeles arbutifolia, the California holly, is an evergreen shrub native to chaparral habitats of Southern California that fruits from November to January. Fruit species morphology has been shown to be a good indicator of habitat quality among other plants. The variation in fruit morphology was examined for the native plant at two diverse Southern California sites, a wetland and a canyon. California Holly fruits had a significantly greater volume in Ballona Wetlands than at Temescal Canyon (Z= 4.367, P

Interaction Between Differentiating Cell- And Niche-Derived Signals In Hematopoietic Progenitor Maintenance, Bama Charan Mondal, Tina Mukherjee, Lolitika Mandal, Cory J. Evans, Sergey A. Sinenko, Julian A. Martinez-Agosto, Utpal Banerjee

Biology Faculty Works

Maintenance of a hematopoietic progenitor population requires extensive interaction with cells within a microenvironment or niche. In the Drosophila hematopoietic organ, niche-derived Hedgehog signaling maintains the progenitor population. Here, we show that the hematopoietic progenitors also require a signal mediated by Adenosine deaminase growth factor A (Adgf-A) arising from differentiating cells that regulates extracellular levels of adenosine. The adenosine signal opposes the effects of Hedgehog signaling within the hematopoietic progenitor cells and the magnitude of the adenosine signal is kept in check by the level of Adgf-A secreted from differentiating cells. Our findings reveal signals arising from differentiating cells that …

Nitrogen Addition Shapes Soil Phosphorus Availability In Two Reforested Tropical Forests In Southern China, Xiankai Lu, Jiangming Mo, Frank S. Gilliam, Hua Fang, Feifei Zhu, Yunting Fang, Wei Zhang, Juan Huang

Biological Sciences Faculty Research

Scant information is available on how soil phosphorus (P) availability responds to atmospheric nitrogen (N) deposition, especially in the tropical zones. This study examined the effect of N addition on soil P availability, and compared this effect between forest sites of contrasting land-use history. Effects of N addition on soil properties, litterfall production, P release from decomposing litter, and soil P availability were studied in a disturbed (reforested pine forest with previous understory vegetation and litter harvesting) and a rehabilitated (reforested mixed pine/broadleaf forest with no understory vegetation and litter harvesting) tropical forest in southern China. Experimental N-treatments (above ambient) …

Isolation And Characterization Of Bacterial Symbionts From Crotalaria Spectabilis Grown On Trichloroethene Contaminated Soil, April Lyn Rocha

Masters Theses

"The discovery of several members of the Leguminosae family growing on a TCE contaminated site demonstrated that they have the capabilities to tolerate the TCE contamination. This led to an investigation of the plants' ability to metabolize TCE. Research by Dr. Lee Newman showed that nodules from Crotalaria spectabilis grown on the site had TCE metabolites present while nodules from other legumes at the site did not. The presence of TCE metabolites within the C. spectabilis nodules suggests that the bacterial symbiont may be responsible for TCE metabolism. This led us to investigate the symbiont of C. spectabilis and its …

Β3-Adrenergic Agonists Mimic Eustress Response And Reduce Leptin-Mediated Proliferation In A Gbm Cell Line , Johnathan Lawrence, Nicholas J. Cook, Richard A. Rovin, Robert J. Winn

Journal Articles

A great deal of mental stress, depression, and anxiety often overwhelm cancer patients; those diagnosed with glioblastoma multiforme (GBM) are no exception. Different types of stress invariably impact what has been termed “the brain-adipocyte BDNF/leptin axis” (Dr. Cao and colleagues of the Comprehensive Cancer Center at The Ohio State University). For example, eustress (good stress) and distress (bad stress) both lead to increased sympathetic activity and adrenal gland stimulation, yet eustress reduces leptin levels and attenuates tumor growth while distress increases leptin levels and augments tumor growth. Complicating matters in GBM is that leptin and its receptor are expressed at …

"Evidence That Salt Water May Not Be A Barrier To The Dispersal Of Asian Freshwater Crabs" , Lara J. Esser, Neil Cumberlidge

Journal Articles

No abstract provided.

Class Discovery And Prediction Of Tumor With Microarray Data, Bo Liu

All Graduate Theses, Dissertations, and Other Capstone Projects

Current microarray technology is able take a single tissue sample to construct an Affymetrix oglionucleotide array containing (estimated) expression levels of thousands of different genes for that tissue. The objective is to develop a more systematic approach to cancer classification based on Affymetrix oglionucleotide microarrays. For this purpose, I studied published colon cancer microarray data. Colon cancer, with 655,000 deaths worldwide per year, has become the fourth most common form of cancer in the United States and the third leading cause of cancer - related death in the Western world. This research has been focuses in two areas: class discovery, …

Analysis Of The Genetic Structure Of Bithynia Tentaculata Snail Populations In Wisconsin And Minnesota, Sarah J. Whalen

All Graduate Theses, Dissertations, and Other Capstone Projects

In recent years, there have been tens of thousands of waterfowl mortalities in Wisconsin and Minnesota. An invasive species of snail, Bithynia tentaculata, is a host for the trematode parasites (Cyathocotyle bushiensis and Sphaeridiotrema globulus) that have caused these deaths. A microsatellite-enriched genomic library was detected using DNA from a B. tentaculata specimen from Lake Onalaska (Pool 7 of the Upper Mississippi River). Seven polymorphic microsatellite loci were used to genotype snails collected from Lake Butte des Morts, Shawano Lake, and Lake Onalaska in Wisconsin, as well as Lake Winnibigoshish in Minnesota. The genetic diversity of each population was measured …

Functional Characterization Of Putative Cilia Genes By High-Content Analysis, Cary Lai, Nidhi Gupta, Xiaohui Wen, Linda Rangell, Ben Chih, Andrew S. Peterson, J Fernando Bazan, Li Li, Suzie J. Scales

Biology Faculty Publications

Cilia are microtubule-based protrusions from the cell surface that are involved in a number of essential signaling pathways, yet little is known about many of the proteins that regulate their structure and function. A number of putative cilia genes have been identified by proteomics and comparative sequence analyses, but functional data are lacking for the vast majority. We therefore monitored the effects in three cell lines of small interfering RNA (siRNA) knockdown of 40 of these genes by high-content analysis. We assayed cilia number, length, and transport of two different cargoes (membranous serotonin receptor 6-green fluorescent protein [HTR6-GFP] and the …

Quantifying The Impact Of Gene Flow On Phenotype-Environment Mismatch: A Demonstration With The Scarlet Monkeyflower Mimulus Cardinalis, John R. Paul, Seema N. Sheth, Amy L. Angert

Biology Faculty Publications

Geographic range margins offer testing grounds for limits to adaptation. If range limits are concordant with niche limits, range expansions require the evolution of new phenotypes that can maintain populations beyond current range margins. However, many species' range margins appear static over time, suggesting limits on the ability of marginal populations to evolve appropriate phenotypes. A potential explanation is the swamping gene flow hypothesis, which posits that asymmetrical gene flow from large, well-adapted central populations prevents marginal populations from locally adapting. We present an empirical framework for combining gene flow, environment, and fitness-related phenotypes to infer the potential for maladaptation, …

The Effects Of Seasonal Changes And Turbidity On Swimming Performance In White Crappie Pomoxis Annularis And Black Crappie Pomoxis Nigromaculatus., Caleb Joseph Gaston

Electronic Theses and Dissertations

Factors that affect swimming performance in fish can also affect fitness. I tested the effects of time of day, season and turbidity on swimming performance in black crappie Pomoxis nigromaculatus and white crappie Pomoxis annularis. These closely related fish co-occur and their relative abundance is thought to be governed by the levels of turbidity in the form of suspended sediments. Black crappie predominate in clear bodies of water and exhibited significantly greater critical swimming speeds in clear water than did white crappie during spring days and winter nights. A significant reduction in critical swimming speed was observed in black crappie …

Population Biology Of A Coastal Dune-Dwelling Spider (Arctosa Sanctaerosae) Along The Hurricane Disturbed Northern Gulf Of Mexico Coast, Robert A. Hataway

Electronic Theses and Dissertations

With the continued increase in the number of tourists visiting the Northern Gulf coast in the last century and the resulting development of this coastline the habitat of Arctosa sanctaerosae has become fragmented; and the sprawl of large cities along the coast has further degraded available habitat. In addition to anthropogenic disturbance to this coastal region, hurricanes are an additional and natural perturbation to the ecosystem. This habitat has seen a number of major tropical storms over the last decade and I have sought to explore the impact of habitat destruction and storm-induced disturbance on a species of spider endemic …

Efficacy Of Constructed Wetlands Of Various Depths For Natural Amphibian Community Conservation, Andrea Nicole Drayer

Online Theses and Dissertations

Wetlands provide critical habitat for a diverse group of amphibians and provide important ecosystem functions and services to humans. Despite this, most natural wetlands have been lost to land use practices. Consequently, constructing wetlands has become a common practice to mitigate for removed wetlands and to manage for wildlife. There were three primary objectives of this research: 1) to examine whether or not constructed wetlands located on ridge tops in eastern Kentucky in the Daniel Boone National Forest (DBNF) had amphibian communities comparable to natural ephemeral wetlands, 2) to examine amphibian predator-prey relationships within the constructed wetlands, and 3) to …

Phospholipid Analysis In Trypanosoma Cruzi, Melissa Rashonda Harris

Open Access Theses & Dissertations

Chagas disease, caused by the flagellate protozoan Trypanosoma cruzi is one of the most endemic and deadly infectious diseases. T.cruzi is distributed throughout most of South and Central America, where it infects 12 to 19 million people, with an annual incidence of 561,000. Another 35 million are exposed to infection, 2 to 3 million people have clinical symptoms of chronic Chagas disease, and about 45,000 of these may die every year due to cardiac failure. In addition to the growing number of incidences in other countries the disease is an emerging infectious disease in the U.S due to the migration …

Relative Changes In Krillabundance Inferred From Antarctic Fur Seal, T. Huang, L. Sun, John M. Stark, Y. Wang, Z. Cheng, Q. Yang, S. Sun

Biology Faculty Publications

Antarctic krill Euphausia superba is a predominant species in the Southern Ocean, it is very sensitive to climate change, and it supports large stocks of fishes, seabirds, seals and whales in Antarctic marine ecosystems. Modern krill stocks have been estimated directly by net hauls and acoustic surveys; the historical krill density especially the long-term one in the Southern Ocean, however, is unknown. Here we inferred the relative krill population changes along the West Antarctic Peninsula (WAP) over the 20th century from the trophic level change of Antarctic fur seal Arctocephalus gazella using stable carbon (d13C) and nitrogen (d15N) isotopes of …

Pcr Detection Of Nearly Any Dengue Virus Strain Using A Highly Sensitive Primer ‘Cocktail’, Charul Gijavanekal, Maria Anez-Lingerfelt, Chen Feng, Catherine Putonti, George E. Fox, Aniko Sabo, Yuriy Fofanov, Richard C. Wilson

Bioinformatics Faculty Publications

PCR detection of viral pathogens is extremely useful, but suffers from thechallenge of detecting the many variant strains of a given virus that ariseover time. Here, we report the computational derivation and initial experi-mental testing of a combination of 10 PCR primers to be used in a singlehigh-sensitivity mixed PCR reaction for the detection of dengue virus. Pri-mer sequences were computed such that their probability of misprimingwith human DNA is extremely low. A ‘cocktail’ of 10 primers was shownexperimentally to be able to detect cDNA clones representing the four sero-types and dengue virus RNA spiked into total human whole blood …

Invasion By Exotic Earthworms Alters Litter- And Soil-Dwelling Oribatid Mites, Jordan L. Burke, John C. Maerz, Joseph Milanovich, Melanie C. Fisk, Kamal J.K. Gandhi

Biology: Faculty Publications and Other Works

Exotic earthworms are drivers of biotic communities in invaded North American forest stands. Here we used ecologically important oribatid mite (Arachnida: Acari) communities, as model organisms to study the responses of litter- and soil-dwelling microarthropod communities to exotic earthworm invasion in a northern temperate forest. Litter- and soil-dwelling mites were sampled in 2008–2009 from forest areas: (1) with no earthworms; (2) those with epigeic and endogeic species, including Lumbricus rubellus Hoffmeister; and (3) those with epigeic, endogeic, and anecic earthworms including L. terrestris L. Species richness and diversity of litter- and soil-dwelling (0–2 cm soil depth) oribatid mites was 1–2 …

Homoplastic Microinversions And The Avian Tree Of Life, Edward L. Braun, Rebecca T. Kimball, Kin-Lan Han, Naomi R. Iuhasz-Velez, Amber J. Bonilla

Biology: Faculty Publications and Other Works

Background

Microinversions are cytologically undetectable inversions of DNA sequences that accumulate slowly in genomes. Like many other rare genomic changes (RGCs), microinversions are thought to be virtually homoplasy-free evolutionary characters, suggesting that they may be very useful for difficult phylogenetic problems such as the avian tree of life. However, few detailed surveys of these genomic rearrangements have been conducted, making it difficult to assess this hypothesis or understand the impact of microinversions upon genome evolution.

Results

We surveyed non-coding sequence data from a recent avian phylogenetic study and found substantially more microinversions than expected based upon prior information about vertebrate …