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Proteomic Investigation Of Aging And Omega-3 Supplementation In Rat Skeletal Muscle, Francesco Maria Del Re, David W. Russ, Kalina P. Dimova, Stylianos P. Scordilis May 2025

Proteomic Investigation Of Aging And Omega-3 Supplementation In Rat Skeletal Muscle, Francesco Maria Del Re, David W. Russ, Kalina P. Dimova, Stylianos P. Scordilis

Biological Sciences: Faculty Publications

Aging is a pan-organ process with an intricate and multimodal nature. Deciphering the aging phenomenon is complex, yet recent attention is analyzing the potential benefits of non-invasive life adjustments to achieve healthy aging. Omega-3 polyunsaturated fatty acids (FA) have emerged as promising nutraceuticals for a plethora of different medical conditions. In the current study we conducted an in-depth, bottom-up, global, shotgun proteomic study (LC-MS/MS) investigating both the effects of aging on skel- etal muscle and the potential alterations due to ω-3 FA. Sprague Dawley rats were fed different diets and divided into four groups (n = 5 per group): adult …

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The Aquatic Symbiosis Genomics Project: Probing The Evolution Of Symbiosis Across The Tree Of Life, Matthew Berriman, Victoria Mckenna, Roxanne Beinart, John M. Archibald, Ute Hentschel, Michael N. Dawson, Jose V. Lopez, Patrick J. Keeling, Jillian M. Petersen, Anne W. Thompson, Multiple Addiional Authors May 2025

The Aquatic Symbiosis Genomics Project: Probing The Evolution Of Symbiosis Across The Tree Of Life, Matthew Berriman, Victoria Mckenna, Roxanne Beinart, John M. Archibald, Ute Hentschel, Michael N. Dawson, Jose V. Lopez, Patrick J. Keeling, Jillian M. Petersen, Anne W. Thompson, Multiple Addiional Authors

Biology Faculty Publications and Presentations

We present the Aquatic Symbiosis Genomics Project, a global collaboration to generate high quality genome sequences for a wide range of eukaryotes and their microbial symbionts. Launched under the Symbiosis in Aquatic Systems Initiative of the Gordon and Betty Moore Foundation, the ASG Project brings together researchers from across the globe who hope to use these reference genomes to augment and extend their analyses of the dynamics, mechanisms and environmental importance of symbioses. Applying large-scale, high-throughput sequencing and assembly technologies, the ASG collaboration will assemble and annotate the genomes of 500 symbiotic organisms – both the “hosts” and the microbial …

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Tickborne Colpodella Species Infections: Time For A New Integrated Approach To Understand Transmission And Pathogenicity, T. Y. Sam-Yellowe, Trinity Nims, Sona Qaderi, Mary M. Asraf May 2025

Tickborne Colpodella Species Infections: Time For A New Integrated Approach To Understand Transmission And Pathogenicity, T. Y. Sam-Yellowe, Trinity Nims, Sona Qaderi, Mary M. Asraf

Biological, Geological, and Environmental Faculty Publications

Colpodella species are free-living predatory protists that prey on algae, ciliates and bodonids using myzocytosis. Colpodella species have been reported in human and animal infections. Polymerase chain reaction (PCR) using primers targeting 18S rRNA genes of Cryptosporidium and piroplasms have identified Colpodella species in arthropods, host blood and feces, demonstrating the phylogenetic closeness of Colpodella species to the apicomplexa. However, in human, animal and arthropod hosts, the life cycle stages of Colpodella are unknown. In this literature review, we provide an overview of the widespread occurrence of Colpodella species in ticks, and pathogenicity in humans and animals. We discuss methods …

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Sustainable Management Of Copper-Contaminated Soils Using Vetiver Root Biochar, Sameer Neve, Dibyendu Sarkar, Zhiming Zhang, Rupali Datta May 2025

Sustainable Management Of Copper-Contaminated Soils Using Vetiver Root Biochar, Sameer Neve, Dibyendu Sarkar, Zhiming Zhang, Rupali Datta

Michigan Tech Publications

Throughout the 19th and early 20th centuries, copper mining was a predominant industry in Michigan's Upper Peninsula, resulting in substantial environmental issues due to the generation of mining waste. This waste, referred to as stamp sands, was disposed of in Lake Superior, causing severe harm to aquatic ecosystems. Later, the stamp sands were dredged and deposited along the lake shores, causing large-scale contamination with copper. This rendered the land incapable of supporting vegetation. This study investigates the efficacy of vetiver (Chrysopogon zizanioides) root biochar as a soil amendment to enhance the quality of copper-contaminated stamp sands. The biochar was produced …

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Ammonia Regulation In Aedes Taeniorhynchus, A Salt-Water Mosquito, Joseph Edmonds May 2025

Ammonia Regulation In Aedes Taeniorhynchus, A Salt-Water Mosquito, Joseph Edmonds

Undergraduate Honors Theses

The salt tolerant mosquito species Aedes taeniorhynchus has been observed in high salinity and ammonia conditions. Previous studies indicate Na+/K+ ATPase (NKA), the proton pump vacuolar-type H+ ATPase (VHA) and sodium-dependent cation-chloride cotransporters (NKCC’s) in sodium secretion in larval Aedes taeniorhynchus. However ammonia transporter such as Rh-protein, Amt1, and Amt2 have only been characterized in Aedes taeniorhynchus’s fresh water relative Aedes aegypti. Our research exhibits the expression of these transporters in A.taeniorhynchus using immunohistochemistry techniques. Evidence of VHA and NKA function in ammonia transport was characterized in vivo using drug antagonists in varying saline and ammonia concentrations. Ammonia flux rates …

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Color Tests For The Presumptive Identification Of A Range Of Benzodiazepines, Regan Mulvin May 2025

Color Tests For The Presumptive Identification Of A Range Of Benzodiazepines, Regan Mulvin

Honors Projects

Color tests are presumptive chemical tests that determine the class of drug in both laboratory and field settings. Despite the rising popularity of benzodiazepines and the usefulness of color tests, there is no widely accepted color test for identifying them. This study tested six different color tests against five benzodiazepines, including diazepam, clonazepam, lorazepam, alprazolam, and flualprazolam, to identify which of the tests would work most generally across all benzodiazepines. Of the six tests, the McKibben test, the Janovsky test, and the Zimmerman test all showed potential usefulness as a presumptive color test. More studies should be done on a …

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The Impact Of Growth Hormone-Releasing Hormone Antagonist On Human Adipose-Derived Stem Cells, Jeanne Michelle Dugas May 2025

The Impact Of Growth Hormone-Releasing Hormone Antagonist On Human Adipose-Derived Stem Cells, Jeanne Michelle Dugas

Master's Theses

Obesity is a condition that affects over 40% of American adults and contributes to numerous preventable health issues. This condition is caused by increased adipose tissue, which forms when stem cells transform into adipocytes. Mesenchymal stem cells, including human adipose-derived stem cells (hASC), are multipotent with the ability to differentiate into adipocytes, osteocytes, or chondrocytes. Many factors affect this transformation, including the peptide growth hormone-releasing hormone (GHRH). The main role of GHRH is to trigger the release of growth hormone, but it has also been found to play an important role in adipocyte metabolism. We expect that if the differentiation …

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The Antioxidant Potential Of Rosemary Leaf-Derived Nanovesicles, Shani Akila Griffin May 2025

The Antioxidant Potential Of Rosemary Leaf-Derived Nanovesicles, Shani Akila Griffin

Master's Theses

Human dermal fibroblasts are essential for maintaining skin homeostasis. However, exposure to exogenous and endogenous stressors can lead to fibroblast instability, extracellular matrix degradation, and excessive reactive oxygen species (ROS) production. These factors contribute to skin aging and ROS-induced skin disorders leading to hair loss. Adverse effects from synthetic treatments have driven interest in natural alternatives like plant extracts for skin therapy. However, the polyphenols in these treatments require an effective delivery system to ensure their stability and targeted application. Plant derived nanovesicles (PDNVs) are emerging as a promising natural alternative for therapeutic applications. Their biocompatibility, stability, and encapsulated compounds …

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Global Maintenance Of Histone Post-Translational Modifications During The Transition Into Anoxia In Embryos Of The Annual Killifish, Chelsea Hughes, Elizabeth A. Mojica, Dietmar Kültz, Jason E. Podrabsky May 2025

Global Maintenance Of Histone Post-Translational Modifications During The Transition Into Anoxia In Embryos Of The Annual Killifish, Chelsea Hughes, Elizabeth A. Mojica, Dietmar Kültz, Jason E. Podrabsky

Biology Faculty Publications and Presentations

Many organisms have adapted to survive anoxic or hypoxic environments, but the epigenetic responses involved in this successful stress response are not well described in most species. Embryos of the annual killifish have the greatest tolerance to anoxia of all vertebrates, making them a powerful model to study the cellular mechanisms necessary for anoxia tolerance. However, the global histone landscape of this species has never been quantified or explored in relation to stress tolerance. Liquid chromatography-mass spectrometry and a Python bioinformatics workflow were used to identify histones and their post-translational modifications. This pipeline resulted in the detection of 252 unique …

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Transformer Decoder-Enhanced Swin Unetr For Multi-Organ Semantic Segmentation On Openkbp: Improving Radiotherapy Planning Accuracy, Zainab Adnan Jwad, Israa Hadi Ali May 2025

Transformer Decoder-Enhanced Swin Unetr For Multi-Organ Semantic Segmentation On Openkbp: Improving Radiotherapy Planning Accuracy, Zainab Adnan Jwad, Israa Hadi Ali

Karbala International Journal of Modern Science

Accurate segmentation of organs-at-risk (OARs) in head and neck CT scans is crucial for radiotherapy planning. The CNN-based decoder limitation of Swin UNETR hinders its capacity to process meaningful information from multiple organ positions essential for accurate medical segmentation. The proposed Transformer Decoder-enhanced Swin UNETR model targets the OpenKBP dataset multi-organ segmentation through its dedicated design for this purpose. The model utilizes transformers along with cross-attention approaches in its decoder to improve segmentation mask outputs through analysis of extensive global information. The model gets additional feature representation power through the addition of squeeze-and-excitation (SE) blocks linked with spatial attention mechanisms …

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Computational Approach: 3d-Qsar, Molecular Docking, Molecular Dynamics Simulation Investigations, Drug- Like-Ness, And Dft Score Evaluation Of A Potential Novel And Retrosynthesis Of Some Tr-H Derivatives As Streptococcus Pneumoniae Drug., Belaidi Mustapha, Tchouar Noureddine, Djelilate Mohammed, Saleh Bufarwa, Dalal K. Thbayh May 2025

Computational Approach: 3d-Qsar, Molecular Docking, Molecular Dynamics Simulation Investigations, Drug- Like-Ness, And Dft Score Evaluation Of A Potential Novel And Retrosynthesis Of Some Tr-H Derivatives As Streptococcus Pneumoniae Drug., Belaidi Mustapha, Tchouar Noureddine, Djelilate Mohammed, Saleh Bufarwa, Dalal K. Thbayh

Karbala International Journal of Modern Science

Streptococcus pneumoniae is the main source of hospital-acquired pneumonia and meningococcal pneumonia in children, adults, and the elderly, especially the immunocompromised. Recently, it has attracted the attention of many research studies around the world as a potential target for remediation. TR-H are considered SP antibacterial agents, as they can inhibit. To describe their mode of action and to identify new antibacterial drugs against Streptococcus pneumoniae exhibiting the TR-H scaffold, the present work included 3D-QSAR, in- silico pharmacokinetic evaluation and molecular simulation modelling of TR-H. Using an atom-based method for quantitative structure-activity relationship (QSAR) study, a comprehensive 3D-QSAR model …

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Understanding Host-Pathogen Interactions Using The Caenorhabditis Elegans – Orsay Virus Experimental Model, Chika Fujii May 2025

Understanding Host-Pathogen Interactions Using The Caenorhabditis Elegans – Orsay Virus Experimental Model, Chika Fujii

Arts & Sciences Graduate Student Theses and Dissertations

Over an evolutionary timeline, viruses have evolved ways to exploit biochemical pathways of the host for their propagation, whereas host organisms developed mechanisms to recognize virus infection and cope with them. Consequently, a better understanding of the different host factors involved in virus replication could provide insights into potential biological pathways or molecules for therapeutic targeting. Furthermore, detailed dissection of the mechanisms of virus sensing could reveal fundamentally important aspects of virus sensing that are evolutionarily conserved to mammals and offer insights into potentially underexplored areas of research. The C. elegans experimental model offers a useful platform for studying evolutionarily …

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Comparing Performance Of Aquaponics Systems Using Different Designs And Fish Feeds, Cody G. Willmore May 2025

Comparing Performance Of Aquaponics Systems Using Different Designs And Fish Feeds, Cody G. Willmore

Biology Theses, Dissertations, and Student Creative Activity

Future food production requires sustainable approaches that used both land and water resources efficiently, while remaining economically viable. One approach is aquaponics, the practice which combines hydroponics and aquaculture, as a sustainable food producing system. Aquaponic systems are unique, as the system can be designed to reuse and repurpose locally sourced materials and resources to meet individual needs. However, productivity of these systems can be inefficient or inconsistent, impacting the viability of aquaponics. The first objective of my study was to compare how system design between a do-it yourself reclaimed-materials aquaponic system and a prefabricated aquaponic setup affects water quality …

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Network Analysis Of Antimicrobial Resistance In Staphylococcus Aureus: Characterization Of Hub Genes And Their Functional Implications, Md Imran Hasan, Davida Smyth, Jeong Yang, Ashley Teufel May 2025

Network Analysis Of Antimicrobial Resistance In Staphylococcus Aureus: Characterization Of Hub Genes And Their Functional Implications, Md Imran Hasan, Davida Smyth, Jeong Yang, Ashley Teufel

Masters Theses (Archived)

Antimicrobial resistance is a major cause of morbidity and mortality in patients with S. aureus infections. In this study, we analyzed genes, molecular mechanisms, and pathways driving drug resistance in S. aureus using network analysis. Using whole-genome sequencing (WGS) data and systems biology approaches, we identified 229 AMR-associated genes and constructed a protein-protein interaction network among these genes. Through network topology and functional enrichment analyses, we not only confirmed their association with resistance, but also highlighted the central roles of these genes in resistance pathways, such as efflux, target replacement, and target protection, which are directly linked to multiple drug …

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Thermal Physiology Of The Giant Day Gecko, Phelsuma Grandis, Infinity Alvarez May 2025

Thermal Physiology Of The Giant Day Gecko, Phelsuma Grandis, Infinity Alvarez

Masters Theses (Archived)

Ectotherms rely on their environment to regulate and maintain their body temperature. For this reason, environmental fluctuations strongly impact their biology. Lizards are ectotherms that are suitable models to investigate thermal physiology. However, the bulk of this research has been studied using new world Anolis lizards. In this study, the giant day gecko, (P.grandis), native to Madagascar but invasive in Florida, was selected as a model for comparison. In our physiotype of P.grandis we included measurements such as metabolic rates, sprint speed at relevant temperatures, thermal preference, critical thermal minimum, and voluntary thermal maximum. Our findings showed that …

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Impacts Of Land Development On Soil Microdiversity And Antibiotic Production, Dennis Guillen Castro, Robert Garcia, Jonathan Castro, Davida Smyth May 2025

Impacts Of Land Development On Soil Microdiversity And Antibiotic Production, Dennis Guillen Castro, Robert Garcia, Jonathan Castro, Davida Smyth

Masters Theses (Archived)

The impact of land development on soil microbial diversity and antibiotic production is critical due to industrial expansion and the scarcity of effective antibiotics. Low Impact Development (LID) aims to preserve ecosystems, but its effectiveness in maintaining microbial diversity is uncertain. This study compares soil samples from nine sites: undisturbed (UD), residential (RD), and Madla Greenway (GW) within the VIDA development area and Texas A&M University-San Antonio campus. Isolated microbes were screened for antibiotic production and genetically characterized. Preliminary findings indicate that UD sites harbor greater microbial abundance, and a higher proportion of antibiotic-producing microbes compared to GW and RD …

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Mate Choice And Movement Ecology Of Plegadis Ibis In Louisiana, Ryan T. Klutts May 2025

Mate Choice And Movement Ecology Of Plegadis Ibis In Louisiana, Ryan T. Klutts

LSU Master's Theses

In 1817, George Ord documented the first North American records of Glossy Ibis (Plegadis falcinellus), a species native to the Old World. Over the next 200 years, Glossy Ibis expanded its breeding range to include the Atlantic coast to southernmost Florida and thence up the Gulf coast to eastern Texas. Forty-five to 75 years ago, its range expansion brought it into secondary contact with White-faced Ibis (P. chihi) in Louisiana, a native species whose breeding range includes freshwater wetlands in Louisiana and eastern Texas. Hybridization between the two species resulted in a large hybrid zone in southern Louisiana, providing an …

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Pollinator Plant Communities Within Restored Montane Longleaf Pine Ecosystems, Hanen Van Rossem May 2025

Pollinator Plant Communities Within Restored Montane Longleaf Pine Ecosystems, Hanen Van Rossem

Master's Theses

Historically the fire-dependent and biodiverse longleaf pine (Pinus palustris) ecosystem was the dominant ecosystem of the Southeastern US. This ecosystem’s range was massively reduced due to land conversion and fire exclusion, causing the decline of many native species. Efforts to restore and protect longleaf pine stands are now ongoing with the goal of expanding habitat and refuge for threatened species. Differences in restoration practices and prior land use may influence the extent to which restored habitat patches can support pollinators and their plants. This study compared pollinator plant biodiversity between restored neighboring montane longleaf pine stands that differed in …

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Oviposition And Immature Development Of Blowflies In Forensic Investigations, Amalia Herrera-Benitez May 2025

Oviposition And Immature Development Of Blowflies In Forensic Investigations, Amalia Herrera-Benitez

Themis: Research Journal of Justice Studies and Forensic Science

Forensic entomology is a subspecialty within the field of forensic science where insect evidence is analyzed to solve criminal cases. Insects begin to colonize the body, with blowflies being the first insects to arrive. Within minutes, blowflies can lay eggs on a decomposing body, and the larvae will feed once they hatch. The life cycles of blowflies are analyzed to help determine an estimated post-mortem interval (PMI), which is crucial in investigations, as it can help establish a timeline. Various factors, such as temperature and access to the body, can cause delays in the oviposition of blowflies. A delay in …

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A Phylogenomic Investigation Of The Historical Biogeography Of Livebearers (Cyprinodontiformes: Poeciliidae), Sheila Rodríguez Machado May 2025

A Phylogenomic Investigation Of The Historical Biogeography Of Livebearers (Cyprinodontiformes: Poeciliidae), Sheila Rodríguez Machado

LSU Doctoral Dissertations

The Neotropics contain several biodiversity hotspots and harbor the highest diversity of freshwater fishes globally. Yet, recognizing the patterns and processes driving this richness remains a challenge. Similarly, all groups and subregions have not received the same attention, especially in the genomics era. Addressing these knowledge gaps will help us to better understand the region’s evolutionary and biogeographic complexity. The goal of this dissertation is to examine phylogenetic and biogeographic patterns of Neotropical freshwater fishes, with emphasis on the Greater Antilles, across multiple taxonomic and geographic scales. To achieve this goal, I generated a genomic dataset of hundreds of ultraconserved …

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A Floristic Study Of The Vascular Flora Of The Brokeoff Mountains, Southcentral New Mexico, Bryana L. Olmeda, Harpo Faust, Hannah E. Marx May 2025

A Floristic Study Of The Vascular Flora Of The Brokeoff Mountains, Southcentral New Mexico, Bryana L. Olmeda, Harpo Faust, Hannah E. Marx

Biology ETDs

The vascular flora detailed here describes 438 km2 (169 mi2) of the Brokeoff Mountains of southeastern New Mexico. The Brokeoffs are part of the northern extent of the Chihuahuan Desert, the largest desert in North America and one of the ecoregions least represented in botanical collections. Prior to 2023, only 254 plant collections were known from the study site, representing roughly 1.5 collections per 2.59 km2 (1 mi2), despite strong research interest in its neighboring Guadalupe Mountains. Over two field seasons, 1920 plant collections were made, over 81 days of field work conducted, in …

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Generation Of A Transgenic Vector For The Purpose Of Transforming Charcoal Rot Resistance Into Soybeans (Glycine Max), John M. Bear Jr May 2025

Generation Of A Transgenic Vector For The Purpose Of Transforming Charcoal Rot Resistance Into Soybeans (Glycine Max), John M. Bear Jr

Electronic Theses & Dissertations

Charcoal rot, caused by Macrophomina phaseolina, is among the most devastating fungal pathogens affecting G. max, particularly in drought-susceptible regions such as the midwestern United States. Traditional agricultural practices have included crop rotation and irrigation, which have proved ineffective or economically unfeasible. Charcoal rot infects over 500 other species of plants and can overwinter as dormant sclerotia which can germinate once soybeans are re-planted and infect young plants. As a result, transgenic solutions have offered a promising alternative for the development of disease-resistant soybeans. This thesis documents the construction of a transgenic vector to express BOZO, a …

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Balancing Burrowing Owl (Athene Cunicularia Hypugaea) Protections While Maintaining Renewable Energy Development Within The San Joaquin Valley, Madison Lenzi Mcginn May 2025

Balancing Burrowing Owl (Athene Cunicularia Hypugaea) Protections While Maintaining Renewable Energy Development Within The San Joaquin Valley, Madison Lenzi Mcginn

Master's Projects and Capstones

Balancing the needs of wildlife and people has long been a complex challenge. The San Joaquin Valley exemplifies this tension, as space for agriculture and urbanization compete for land use of native grasslands– often resulting in the conversion of grasslands for agriculture fields, commercialized areas, or energy development. With an increasing number of agricultural landscapes going fallow and the passing of SB-100 in 2018, solar farm development has emerged as an attractive option to offset financial losses from unproductive farmland. However, the same landscapes favored for solar development also provide suitable habitat for sensitive species such the western burrowing owl …

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Ecology And Management Of Alligatorweed (Alternanthera Philoxeroides) And An Associated Thrips Biological Control Agent (Amynothrips Andersoni), Samuel Alec Schmid May 2025

Ecology And Management Of Alligatorweed (Alternanthera Philoxeroides) And An Associated Thrips Biological Control Agent (Amynothrips Andersoni), Samuel Alec Schmid

Theses and Dissertations

Alligatorweed (Alternanthera philoxeroides), native to South America, is a highly invasive aquatic macrophyte that is globally distributed and particularly problematic in the southeastern United States. In the United States, alligatorweed management has a long history, particularly of chemical and biological control. Alligatorweed management saw early success with a South American flea beetle (Agasicles hygrophila), but this agent’s utility has waned. Despite the long history of alligatorweed invasion, several important research questions remain, and the research in this dissertation seeks to answer some of these questions. This research focuses on multiple and diverse aspects of alligatorweed biology, ecology, and management: (1) …

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Effects Of Cytochalasin D On Motor Function In Drosophila, Airyel Noguera May 2025

Effects Of Cytochalasin D On Motor Function In Drosophila, Airyel Noguera

Honors Theses

Actin proteins play an important role in microfilaments, one of the three components of the cytoskeleton of the cell. The actin protein is responsible for cellular functions such as with their role in neuromuscular junctions. The dynamics of these proteins can be further explored with manipulation of polymerization through the treatment of a biochemical drug, Cytochalasin D. This fungal toxin caps the ends of actin filaments, destabilizing them by inhibiting G- and F-actin monomers from binding. Treating Drosophila in their early embryonic stage with this drug after permeabilization can give insights on the importance of actin proteins at neuromuscular junctions …

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Turtle Creek Tree Restoration Project, Rebecca Beneroff, Risa Kanefsky, Max Dicerbo, Pj Newhart, Amanda Mitchell May 2025

Turtle Creek Tree Restoration Project, Rebecca Beneroff, Risa Kanefsky, Max Dicerbo, Pj Newhart, Amanda Mitchell

Final Reports in ENST 411: Environmental Community Projects

Over the course of this project our group members Rebecca, Max, PJ, Amanda, and Risa worked closely with Jim Knight and Brian Auman from Turtle Creek Park to help research, plan, and execute a tree transplanting event that would be efficiently implemented and environmentally sound. This work involved extensive preparatory work in the park including soil testing, bare leaf tree identification, tree and shrub species characterization, and recommendation. The entire process was rooted in the most up to date literature and scientific practices surrounding bare root tree nurseries, existing land management plans, invasive species and public park management, ecological succession, …

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Mechanisms That Contribute To Age-Dependent Segregation Errors In Drosophila Oocytes, Zihan Meng May 2025

Mechanisms That Contribute To Age-Dependent Segregation Errors In Drosophila Oocytes, Zihan Meng

Dartmouth College Ph.D Dissertations

Meiotic chromosome segregation errors in human oocytes are the leading cause of miscarriages and aneuploid pregnancies, and these errors increase dramatically as women age. Using Drosophila oocytes as a model system, this dissertation identifies the NAD⁺-dependent deacetylase Sirt1 as a key regulator of chromosome segregation and meiotic arm cohesion. Loss of Sirt1 activity during meiotic prophase leads to premature loss of arm cohesion and increased segregation errors in Drosophila oocytes. In addition, elevated acetylation of its substrate, histone H4K16, indicates that Sirt1 activity declines in oocytes during aging. Strikingly, dietary administration of the Sirt1 activator SRT1720 preserves Sirt1 deacetylase activity …

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Dna Extraction And Species Determination Of Historic Museum Pelt Samples, Selin Ates May 2025

Dna Extraction And Species Determination Of Historic Museum Pelt Samples, Selin Ates

Student Theses

The identity of the fur covering the sculpture named Object in the Museum of Modern Art has remained a mystery since its creation in 1936 by Meret Oppenheim. DNA testing can possibly provide an answer, but to preserve the integrity of the sculpture, only a very small piece of the fur can be sampled. Prior to testing Object, testing using museum samples was performed to determine whether DNA of a sufficient quantity and quality can be extracted and sequenced. Several DNA extraction and sample washing methods were tested to examine the effects on the DNA of museum pelts aged …

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A Bitter Brew For Cancer: Egcg’S Impact On Ewing Sarcoma Cells, Lizzeth Holguin, Mary-Esther Leblanc, Mariana Reyes, Terry Jo Shackleford May 2025

A Bitter Brew For Cancer: Egcg’S Impact On Ewing Sarcoma Cells, Lizzeth Holguin, Mary-Esther Leblanc, Mariana Reyes, Terry Jo Shackleford

Cell and Molecular Methods

Epigallocatechin Gallate (EGCG), a powerful antioxidant found in green tea, is an abundant treatment to treat Ewing Sarcoma cells, a pediatric cancer affecting the skeletal system. Given EGCG’s known role in other cancer treatments, these tests aim to investigate its effects as a standalone natural compound on Ewing Sarcoma cells. Focusing on the PI3K/AKT pathway, if the PI3K/AKT pathway is inhibited by EGCG, then it will lead to reduced cell survival and proliferation of EW8 cells. Using cell culture techniques, IncuCyte-based IC50 analysis, caspase 3/7, RNA purification, cDNA synthesis, and qRT-PCR, EGCG’s impact was assessed on cell apoptosis and gene …

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The Effect Of Epigallocatechin Gallate (Egcg) And Alpha L-Mangostin On Tp53, Bax, And Vim Gene Expression And Apoptosis In Ewing Sarcoma Cells, Victoria Valdez, Terry Jo Shackleford May 2025

The Effect Of Epigallocatechin Gallate (Egcg) And Alpha L-Mangostin On Tp53, Bax, And Vim Gene Expression And Apoptosis In Ewing Sarcoma Cells, Victoria Valdez, Terry Jo Shackleford

Cell and Molecular Methods

Ewing Sarcoma (ES) is a malignant pediatric bone tumor driven by chromosomal translocations and fusion oncogenes with limited targeted treatment options. This study investigated the chemopreventive potential of two natural compounds, Epigallocatechin Gallate (EGCG) found in green tea, and Alpha L-Mangostin from mangosteen, on apoptosis and gene expression in ES cells. We hypothesized that EGCG and Alpha L-Mangostin treatment would induce apoptosis and downregulate cancer-promoting genes. To test this, we performed tissue culture, IC50 assays, caspase-based apoptosis detection, RNA purification, cDNA synthesis, and qRT-PCR on ES cell lines treated with a high and low concentration of the two compounds. Our …

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