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Effect Of Matallic Ions, B-Vitamins, & Amino Acids On The Production Of Trans-2, 3-Epoxysuccinic Acid By Paecilomyces Varioti, Eleanor Ling

Masters Theses & Specialist Projects

The nutritional conditions which permit maximum yield of trans-2,3- epoxysuccinic acid by Paecilomyces varioti NPRL 1123 were investigated. Copper and iron ions were added to decationized fermentation media to determine the optimum concentration and ratio of the ions for the accumulation of trans-2,3-epoxysuccinic arid. P-vitamin and amino acid studies were done by single omission of the growth factors in culture media. Organic acids in the cultures were recovered by ether extraction and identified by gas chromatography.

In addition to t-ans-2,3-epoxysuccinic acid, succinic acid, malic acid, and tartaric acid were also found in culture filtrates. The results indicated that with a …

The Effect Of Photoperiod On The Regressive Phase Of The Ovarian Cycle Of The Lizard, Anolis Carolinensis., John Dickens Pinch

Master's Theses

The effects of constant photoperiod (14 hours of light) and decreasing photoperiod (13-8 hours of light) on the regressive phase of the ovarian cycle of the lizard Anolis carolinensis were investigated. Changes in body, liver, ovary and oviduct weights were measured and microscopic examinations of several ovarian parameters were made. In both light regimens body and liver weights decreased over the first four weeks of the experiment but both increased at the sixth week. In addition, ovary weight was highly variable but exhibited a decreasing trend and oviduct weight decreased. There were decreases in the number of previtellogonic follicles and …

The Isolation And Identification Of Yeast-Like Fungi In Non-Active Tuberculosis Patients In Rowan County, Kentucky, Connie Harris Baker

Morehead State Theses and Dissertations

A thesis submitted to Morehead State University in partial fulfillment of the requirements for the degree of Master of Science in Biology by Connie Harris Baker in July of 1976.

Nitrogen Cycling In A Microcosm Simulation Of The Northern Arm Of The Great Salt Lake, John C. Stube

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Nitrogen cycling was studied in the hypersaline northern arm of the Great Salt Lake (332,480 mg/l total dissolved solids) using a sediment-water microcosm simulation of that system. The study demonstrated that nitrate, ammonia, and urea were not stimulating to the halophilic bacteria in the microcosm. The bacteria were simulated only indirectly simulated bacterial growth. In addition, no nitrification could be demonstrated during the study.

Weekly analyses of water samples from the north arm of the lake itself showed that nitrate and nitrite were absent, leaving ammonia as the only significant form of inorganic nitrogen in the northern lake basin. Other …

Ecological Management Of The Alfalfa Leafcutter Bee, Megachile Pacifica (Panzer), With Emphasis On Diapause Induction, Ron M. Bitner

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The effects of photoperiod and temperature on diapause induction in the alfalfa leafcutter bee, Megachile pacifica (Panzer) (=M. rotundata Auct.), were studied during the summers of 1972, 1973 and 1974, The influence of photoperiod and temperature on mortality, rate of development and incidence of diapause was measured during the developmental stages of this insect. The aim of this research was to assess the potential for manipulation of the number of generations of this bee per season so as to develop a practical and ecologically-sound method of management,

Eggs, larvae, pupae and adults of M. pacifica were subjected to regimens …

Insect Consumption Of Seeded Rangeland Herbage In A Selected Area Of Diamond Fork Canyon, Utah County, Utah, Diane M. Bowers

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

This study compares insect and cattle consumption of crested wheatgrass (Agropycon spp.) on a site in Sterling Ranch, Utah County, Utah. The hypothesis tested is that insect consumption in general, and specifically consumption by Labops hesperius Uhler, significantly reduces total available cattle forage.

Labops and grasshoppers were probably the major cause of secondary damage at the study site, while the impact of thrips is unknown. A detrimental impact on range was suggested by the data, as insects consumed forage equivalent to 2.8 animal units per month, while cattle consumed forage equivalent to 2.1 animal units per month at the …

Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoretic Studies Demonstrating Cytoplasmic Protein Components Unique To The Mycelial Phase And To The Yeast Phase Of Candida Albicans, Theresa Catherine Burgess

Biological Sciences Theses & Dissertations

Blastospore and mycelial cytoplasmic extracts from four strains of Candida albicans, varying in virulence, were compared by sodium dodecyl sulfate (SDS) polyacrylamide disc gel electrophoresis Cytoplasmic extracts were made by disrupting the cultures in a Braun homogenizer followed by ultracentrifugation. The supernatant fractions were then resolved on polyacrylamide gels containing SDS. Each of the preparations yielded a distinct pattern. There were mycelial specific and blastospore specific protein bands. There were more components resolved from the blastospore phase cytoplasmic extracts than from the mycelial phase cytoplasmic extracts. There were bands common to both phases. These results demonstrate protein components unique …

Some Effects Of Solid Rocket Motor Fuel Exhausts On Avian Embryos, Lindsey Elliott Deguehery

Retrospective Theses and Dissertations

Fertile White Leghorn (Gallus gallus) and Bobwhite Quail (Colinus virginianus) were subjected to 15 min exposures produced by burning solid rocket motor (SRM) fuel. Comparative mortality data were collected. Chicken eggs were further used to study the effects of exposure on water relations and blood gas parameters. Chicken embryos exposed once on day 19 or incubation demonstrated and LD50 of 204 ppm; the LD50 for quail embryos was 175 ppm. When mortality was regressed on the final exposure concentration, chicken and quail embryos exposed on days 12 and 19 showed LD50's of 127 and 86 ppm respectively, and embryos exposed …

Populations Of Lepomis Auritus (Redbreast Sunfish) In A Thermally Influenced Section Of The James River, Virginia, John Rawls Saecker

Master's Theses

From October 1971 - September 1973, electrofishing was used to collect 1004 specimens of Lepomis auritus (year classes 0 - V) from a thermally influenced section of the James River near Blemo Bluff, Virginia. Collections of fish from the natural and heated water temperature habitats were analyzed as to year classes, season, length and sex.

Effects Of The Toxins Of The Ectoproct, Lophopodella Carteri, On Oxygen Consumption In Gill Tissues Of The Bluegill, Lepomis Macrochirus, Barbara Harper Green

Master's Theses

Large and small bluegill, Lepomis macrochirus, were exposed to homogena tes of Lophopodella carteri, an ectoproct that contains substances toxic to some gilled vertebrates. Oxygen consumption tests on the excised gill tissues of the bluegill indicated that the ectoproct toxins caused a lowering of oxygen consumption of the gill tissues. Oxygen consumption of the gill tissues of the smaller fish was reduced to a greater extent than that of larger fish. An inverse relationship was found be tween the temperature of the medium and the period of time for death to occur. It is proposed that the toxins cause physiological …

Seasonal And Spatial Variation In Primary Productivity In Boulder Basin, Lake Mead, Clark County, Nevada, Isamu Aoki

Publications (WR)

The ¹⁴C light and dark bottle technique for measurement of primary production was utilized as a means of assessing the amount of inorganic carbon being converted Into organic form by the photosynthesis of phytoplankton populations In the Boulder Basin of Lake Mead.

Spatial and time series changes of productivity levels observed at eight sampling locations within Boulder Basin Indicate that the Influence of treated municipal arts' industrial effluent flowing into Les Vegas Bay is contributing high levels of available nutrients at Las Vegas Wash Inlet to cause productivity to approximate those levels associated with polluted waters.

Productivity levels at …

Cyclic Patterns Of Central Florida Sooty Mold, Bobby Ray Pohlad

Retrospective Theses and Dissertations

This study was conducted to record seasonal variations in the epiphyllous sooty mold colonies found on citrus leaves in two central Florida citrus groves, to determine basic patterns of fungal growth throughout the year and to determine whether these general patterns are correlated with regional climatological data. The results indicated general seasonal variations in the reproductive units of five of the fungi from the colony. In the study, correlations were seen between the two Ascomycetes, Treubiomyces pulcherrimus and Trichomerium didymopanacis. They were found fruiting most abundantly during the winter and spring. Reproductive units of two asexual sooty mold species (Podoxyphium …

A Serological Survey Of Greater Orlando Human, Canine, And Feline Populations For The Presence Of Antibodies To Toxoplasma Gondii, John Louis Weihe

Retrospective Theses and Dissertations

Toxoplasma gondii, the etiological agent of toxoplasmosis, was first discovered in Ctenodactylus gondi, an small North African rodent, by Nicolle and Manceaux in 1908. Later the same year Splendore observed the organism in a laboratory rabbit. Human infection was first reported by Janku in 1923. Since its discovery, many investigations have shown the organism to be truly unique in the protozoan world. There appears to be only one species and only one serotype. Serologic and microscopic studies reveal Toxoplasma to have a vast host range. At least one representative of virtually every mammalian species tested harbored the parasite or showed …

Reproductive Ecology And Population Dynamics Of Two Sympatric Species Of Macoma (Bivalvia), John Gibson Rae Iii

University of the Pacific Theses and Dissertations

Populations of the sympatric intertidal bivalves, Macoma secta and M. nasuta are compared as to reproductive ecology and population dynamics. Histological examination of seasonal gonadal changes indicate that both species undergo dormancy in the winter months and rapid gamete proliferation in the spring. However, the timing of spawning differs. The M. secta population synchronously spawned in August while the M. nasuta population spawned lightly in late spring and more heavily in the fall. Fecundity estimates indicate M. nasuta adds 140% of winter weight in gametes and M. secta only adds 43% of winter weight in gametes. Spat fall was detected …

Lipid And Protein Energy Utilization In Starved Bluegill Sunfish (Lepomis Macrochirus), Nancy H. Siemon

Master's Theses

No abstract provided.

The Effect Of Nematode Age On Trap Formation In The Nematophagous Fungus Arthrobotrys Conoides, Mary Lou Tortorello

Master's Theses

No abstract provided.

Distribution And Effects Of Bacterial Endotoxin, Anne Carter Adams

Theses and Dissertations

In this study the distribution and effects of bacterial endotoxin were examined. Adult male BALB/c and adult male DUB/ICR mice were used in all experiments. Endotoxic activity of a number of strains from three genera of bacteria, Pseudomonas, Bacteroides, and Listeria, was detected using vincristine-treated mice as sensitized indicators for endotoxin and resistance to purified endotoxin induced by pretreatment with selected bacteria. Also, the effect of bacterial endotoxin and endotoxin-like substances on the course of primary amebic meningoencephalitis in mice, caused by Naegleria fowleri, was assessed.

Effect Of "Stressors" On Corticosteroid-Binding Globulin Activity In The Rat During Pregnancy, Muhammad Nasir Hussain

Masters Theses

No abstract provided.

A Study Of Rna Bacteriophage 7s Infection Of Pseudomonas Aeruginosa, Deanne Benson

Dissertations and Theses

A study was conducted to find the effect of magnesium, calcium, manganese and zinc ions on the infection of Psudomonas aeruginosa strain 1C by RNA bacteriophage 7s. When an 18 hour progeny experiment was performed, it was found that magnesium, calcium and manganese had different effects on bacteriophage production and was dependent on the bacterial growth conditions. RNA bacteriophage 7s progeny production was significantly enhanced by the addition of magnesium to cultures of Psudomonas aeruginosa 1C grown in a magnesium deficient medium. Under these environmental conditions there was a slight increase in progeny in the presence of calcium. When Psudomonas …

Enzyme Reactions Using Ureidosuccinate As A Substrate During Pyrimidine Biosynthesis And Degradation In Cl. Oroticum, Penny Amy

Dissertations and Theses

Cells of Clostridium oroticum, an anaerobic bacterium, were grown on orotate as a carbon and energy source. Ureidosuccinase, an inducible enzyme in the pathway for pyrimidine degradation has been shown to convert ureidosuccinate to aspartate, CO₂ and NH₃ as reported by Liebenmm and Kornberg (7). Aspartate and CO₂ were formed in approximately a 1:1 ratio from ureidosuccinase activity.

Ureidosuccinase was found to be a Mn+² requiring enzyme with a pH optimum of approximately pH 6.5. Enzyme activity is labile to O₂, temperature, pH, dilution and high ionic strength. The optimum conditions for storage …

Nocturnal Flights Of Triatoma (Hemiptera: Reduviidae) In Sabino Canyon, Arizona, David B. Ekkens

Loma Linda University Electronic Theses, Dissertations & Projects

Three species of Triatorna were studied in this investigation: T. rubida uhleri (Neiva), _T. protracta protracta (Uhler), and T. recurva (Stal) (Hemiptera:Reduviidae). These blood-sucking bugs are ectoparasites of vertebrate animals. Triatoma rubida uhleri (Neiva) is the most common conenose bug in southern Arizona.

At certain seasons of the year Triatoma bugs enter homes and bite people causing an allergic reaction in sensitized individuals. The purpose of this study was to determine cause of flight, time of day and r season of the year most individuals fly, effects of various physical factors on flights and distances flown.

This research was conducted …

Lesion Formation In The Liver Of Mice Caused By Metabolic By-Products Of Hymenolepsis Microstoma, Gary Simpson

Masters Theses & Specialist Projects

The presence of lesions in the liver of mice is associated with the infection of these mice with the common bile duct -dwelling cestode, Hymenolepis microstoma. Lesions develop even though there is no direct contact between the cestodes and the liver. It was found in this investigation that administration to mice of Tyrode's solution, in which cestodes had been incubated for 24 hr, resulted in liver lesions comparable to those observed in normal infections. The administration of the causative substance by either per os intubation or intraperitoneal injection produced similar lesions. Further studies revealed that the active lesion - …

Characterization Of Selected Bacteria From The North Arm Of The Great Salt Lake, John L. Crane Jr.

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Thirteen bacterial cultures were isolated from the North arm of Great Salt Lake during the months of January and February of 1973. Eleven isolates were gram-negative pleomorphic rods which lysed in hypotonic solution. The remaining two were gram-positive cocci. All isolates and one known strain of Halobacterium salinarium were subjected to examination of morphological, cultural, physiological, and biochemical characteristics. A numerical taxonomic analysis was applied to the compiled characters to compute a coefficient of similarity for each individual isolate as compared to all other isolates. A comparative analysis was included in the similarity computation using characters assembled from those reported …

A Quantitative Study Of The Water Quality And Plankton Of Upper Blue Lake, Lower Blue Lake, And The Oaks Arm Of Clear Lake In Lake County, California, Stephen Patrick Hayes

University of the Pacific Theses and Dissertations

This study was undertaken to examine any possible associations between the measurements of water quality and lake conditions, and the quantity and composition of plankton present in Upper Blue Lake, Lower Blue Lake, and the Oaks Arm of Clear Lake during the sampling period. A secondary objective of this study was to provide a record of these measurements. These measurements, when taken collectively, are important indicators of the trophic state or nutrient condition of the waters of the three lakes from February through October 1972.

Separation Of The Membrane Associated L-Malate And Reduced Nicotinamide Adenine Dinucleotide Dehydrogenases Of Micrococcus Lysodeikticus, Steven S. Dills

Masters Theses

No abstract provided.

Relationships Between Radiophosphorus Accumulation By Bacteria, Algae, And Tubificid Worms In Laboratory Food Chains, Warren L. Stromberg

Masters Theses

No abstract provided.

The Effects Of Atropine On The Growth And Respiration Of Two Species Of Bacteria Normally Inhabiting The Human Gastro-Intestinal System, Mary M. Ramos

Theses & Dissertations

It is the purpose of this study to determine whether the drug atropine has an effect on bacteria found normally inhabiting the human body, most specifically the gastrointestinal system. Atropine is one of the oldest drugs in medicine. It is derived from the roots and leaves of solanaceous plants such as henbane and Jimson weed. Atropine is often termed a belladonna alkaloid because it can also be found in the deadly nightshade plant, Atropa belladonna. Atropine is a highly competitive antagonist of acetylcholine at receptor sites in smooth muscles, cardiac muscle, and various glandular cells (5). Therefore, its effects are …

Time Of Mating And Incidence Of Conception And Implantation In The Post Partum Lactating Female Mouse, Paul A. Walter

Morehead State Theses and Dissertations

A thesis presented to the faculty of the School of Sciences and Mathematics at Morehead State University in partial fulfillment of the requirements for the Degree of Master of Science in Biology By Paul A. Walter on July 23, 1973.

Physiological Comparison Of Plaque-Producing And Non-Plaque-Producing Streptococci With Emphasis On Mannitol Utilization, Brenda Castle

Morehead State Theses and Dissertations

A thesis presented to the faculty of the School of Sciences and Mathematics at Morehead State University in partial fulfillment of the requirements for the Degree of Master of Science in Biology by Brenda Castle on July 19, 1973.

Production Of Iodinated Compounds And The Effect On Strobilation In Aurelia Aurita, William J. Welch

Morehead State Theses and Dissertations

A thesis presented to the faculty of the School of Sciences and Mathematics at Morehead State University in partial fulfillment of the requirements for the Degree of Master of Science in Biology by William J. Welch on May 2, 1973.