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High-Throughput Cultivation Of Bacterioplankton From The Gulf Of Mexico And Genomics Of The First Cultured Ld12 Representative, Michael Winslow Henson Jan 2019

High-Throughput Cultivation Of Bacterioplankton From The Gulf Of Mexico And Genomics Of The First Cultured Ld12 Representative, Michael Winslow Henson

LSU Doctoral Dissertations

Cultivation of microorganisms facilitates characterization of metabolism, interspecies dependencies, virus-host interactions, and other information necessary to resolve the functions and distribution of individual taxa. However, the metabolic and physiological capacities for the majority of microbes remains unresolved because of the lack of cultivated representatives for many groups limits our ability to test cultivation-independent observations. The Northern Gulf of Mexico offers a diversity of ecosystems under the continuous threat from natural and anthropogenic disturbances, yet little is known about its native bacterioplankton community. This dissertation sought to use high-throughput cultivation over three-years at six sites to isolate important coastal bacteria to …


Ancient Polyploidy And Genome Evolution In Palms, Craig F. Barrett, Michael R. Mckain, Brandon T. Sinn, Xue Jun Ge, Yuqu Zhang, Alexandre Antonelli, Christine D. Bacon Jan 2019

Ancient Polyploidy And Genome Evolution In Palms, Craig F. Barrett, Michael R. Mckain, Brandon T. Sinn, Xue Jun Ge, Yuqu Zhang, Alexandre Antonelli, Christine D. Bacon

Faculty & Staff Scholarship

Mechanisms of genome evolution are fundamental to our understanding of adaptation and the generation and maintenance of biodiversity, yet genome dynamics are still poorly characterized in many clades. Strong correlations between variation in genomic attributes and species diversity across the plant tree of life suggest that polyploidy or other mechanisms of genome size change confer selective advantages due to the introduction of genomic novelty. Palms (order Arecales, family Arecaceae) are diverse, widespread, and dominant in tropical ecosystems, yet little is known about genome evolution in this ecologically and economically important clade. Here, we take a phylogenetic comparative approach to investigate …


Is A Whole Food, Plant-Based Diet The Most Effective Treatment Of Arthritis?, Elisheva Liberman Jan 2019

Is A Whole Food, Plant-Based Diet The Most Effective Treatment Of Arthritis?, Elisheva Liberman

The Science Journal of the Lander College of Arts and Sciences

Arthritis is a debilitating disease where joint inflammation occurs. There are different types of arthritis, such as Osteoarthritis and Rheumatoid Arthritis. Osteoarthritis is the destruction of the articular cartilage that usually comes with age, whereas Rheumatoid Arthritis is a chronic autoimmune disease. They both have symptoms which include reduced range of motion, pain and swelling. There are various treatments to alleviate the pain and reduce the inflammation of arthritis. Among them are medication, physical therapy, surgery and a whole food, plant-based diet. The whole food, plant-based diet yields the best results and is the most effective and practical.


Full Issue: Volume 13, Number 1 Jan 2019

Full Issue: Volume 13, Number 1

The Science Journal of the Lander College of Arts and Sciences

Complete .pdf file of Volume 13, Number 1 of The Science Journal of the Lander College of Arts and Sciences.


Mutualist-Provisioned Resources Impact Vector Competency, Rita V. M. Rio, Anna K. S. Jozwick, Amy F. Savage, Afsoon Sabet, Aurelien Vigneron, Yineng Wu, Serap Aksoy, Brian L. Weiss Jan 2019

Mutualist-Provisioned Resources Impact Vector Competency, Rita V. M. Rio, Anna K. S. Jozwick, Amy F. Savage, Afsoon Sabet, Aurelien Vigneron, Yineng Wu, Serap Aksoy, Brian L. Weiss

Faculty & Staff Scholarship

ABSTRACT Many symbionts supplement their host’s diet with essential nutrients. However, whether these nutrients also enhance parasitism is unknown. In this study, we investigated whether folate (vitamin B9) production by the tsetse fly (Glossina spp.) essential mutualist, Wigglesworthia, aids auxotrophic African trypanosomes in completing their life cycle within this obligate vector. We show that the expression of Wigglesworthia folate biosynthesis genes changes with the progression of trypanosome infection within tsetse. The disruption of Wigglesworthia folate production caused a reduction in the percentage of flies that housed midgut (MG) trypanosome infections. However, decreased folate did not prevent MG trypanosomes from migrating …


Are Health Problems In Adulthood Linked To Our Experiences In The Womb? An Epigenetic Approach, Simone Tendler Jan 2019

Are Health Problems In Adulthood Linked To Our Experiences In The Womb? An Epigenetic Approach, Simone Tendler

The Science Journal of the Lander College of Arts and Sciences

The origins of adult disease have been a prime topic for research, as deciphering causes can lead to strategies for preventions and cures. There has been recent intrigue focused on the environment in the womb. Records from England and Wales in 1911 show that those who suffered from cardiovascular disease were geographically correlated with regions high in infant mortality in the past, seventy years before the study. When looking into the cause of the neonatal death rates, low birth weight, poor maternal health, and high maternal death rates during childbirth were clearly associated. Barker inferred that there is much happening …


Prebiotics, Probiotics, And Bacterial Infections, Christina C. Tam, Kirkwood M. Land, Luisa W. Cheng Jan 2019

Prebiotics, Probiotics, And Bacterial Infections, Christina C. Tam, Kirkwood M. Land, Luisa W. Cheng

College of the Pacific Faculty Books and Book Chapters

Bacterial pathogens have developed exquisite virulence mechanisms to survive in the host cells. These virulence mechanisms help them bind and internalize into host cells, replicate, and evade the host immune response. The mammalian host itself has developed its own repertoire of weapons to prevent this from happening. One important component of host response in preventing infections in the gut lumen is the diverse commensal microbiota present. Dysbiosis of the gut microbiota has been implicated in the development of many gastrointestinal diseases. A potential therapeutic pathway to solve these diseases would be by providing probiotics and/or prebiotics to help stimulate growth …


Diversity And Shifts Of The Bacterial Community Associated With Baikal Sponge Mass Mortalities, Sergei Belikov, Natalia Belkova, Tatiana Butina, Lubov Chernogor, Alexandra Martynova-Van Kley, Armen Nalian, Colin Rorex, Igor Khanaev, Olga Maikova, Sergey Feranchuk Jan 2019

Diversity And Shifts Of The Bacterial Community Associated With Baikal Sponge Mass Mortalities, Sergei Belikov, Natalia Belkova, Tatiana Butina, Lubov Chernogor, Alexandra Martynova-Van Kley, Armen Nalian, Colin Rorex, Igor Khanaev, Olga Maikova, Sergey Feranchuk

Faculty Publications

The disease of freshwater sponges was first discovered in 2011, when pink samples were found in the Central Basin of Lake Baikal. Subsequently, the visible signs of the disease have changed, and now sponges appear with various symptoms of damage to the body, such as discoloration, tissue necrosis, the formation of brown patches and dirty-purple biofilms on some branches. These signs of the disease are accompanied by the mass death of sponges. We identified differences in microbiomes by sequencing 16S rRNA genes and found changes in the consortium of microorganisms of freshwater Baikal sponges. We found that the observed imbalance …


Global Temporal Patterns In Plant Nutrient Resorption Plasticity, Rebecca E. Drenovsky, Nicole Pietrasiak, Thomas Short Jan 2019

Global Temporal Patterns In Plant Nutrient Resorption Plasticity, Rebecca E. Drenovsky, Nicole Pietrasiak, Thomas Short

2019 Faculty Bibliography

Aim Leaf nutrient resorption is a key nutrient conservation trait, which also influences nutrient cycling rates and pools. Most global biogeochemical models assume that resorption is non‐varying at a temporal scale. However, this trait can differ substantially within populations among years. We assessed the commonality of attaining proficient resorption, the factors associated with proficient resorption, as well as the variability of this trait and the factors controlling trait variability.


Experimental Evidence For Maternal Provisioning Of Alkaloid Defenses In A Dendrobatid Frog, Ralph Saporito, Matthew W. Russell, Corrine L. Richards-Zawacki, Matthew D. Dugas Jan 2019

Experimental Evidence For Maternal Provisioning Of Alkaloid Defenses In A Dendrobatid Frog, Ralph Saporito, Matthew W. Russell, Corrine L. Richards-Zawacki, Matthew D. Dugas

2019 Faculty Bibliography

Dendrobatid frogs sequester alkaloid defenses from dietary arthropods. Here, we provide experimental evidence that mother strawberry poison frogs (Oophaga pumilio) provision alkaloids to tadpoles. Captive-raised females were fed the synthetic alkaloid decahydroquinoline (DHQ), which we subsequently quantified in their skin, eggs, and developing tadpoles. DHQ quantity was positively associated with tadpole mass/development, suggesting high sequestration rates by tadpoles. These data confirm that tadpoles obtain nutrition and alkaloids by feeding exclusively on maternally provisioned eggs.


Editors’ Picks For 2018 Demonstrate The Diversity Of Research In Regulatory, Integrative, And Comparative Physiology, Willis K. Samson, Roger G. Evans, Wolfgang Langhans, Gina L.C. Yosten Jan 2019

Editors’ Picks For 2018 Demonstrate The Diversity Of Research In Regulatory, Integrative, And Comparative Physiology, Willis K. Samson, Roger G. Evans, Wolfgang Langhans, Gina L.C. Yosten

Biological Sciences Faculty Research & Creative Works

No abstract provided.


The Cost Of Breeding In The Winter Versus The Summer In An Opportunistic, Northtemperate Songbird, The Red Crossbill (Loxia Curvirostra), Travis Draud Jan 2019

The Cost Of Breeding In The Winter Versus The Summer In An Opportunistic, Northtemperate Songbird, The Red Crossbill (Loxia Curvirostra), Travis Draud

Master's Theses and Doctoral Dissertations

Energy is the currency of life, where a surplus allows survival and reproduction and a longstanding debt leads to death, yet monitoring energy expenditures in free-living animals has been relatively limited by available technology. Radio transmitters that have been specially modified to detect heart rate, however, allow for real-time estimation of energy expense in free-living, behaving animals. Red crossbills live at northern latitudes year-round and breed opportunistically throughout much of the year. They therefore offer a unique opportunity to examine the ecophysiology of different life cycle stages under drastically variable seasonal conditions. Here we present heart rate data of free-living, …


Assessing The Prevalence Of Multidrug Resistant Salmonella Enterica From Stream Sediment And Poultry Litter In The Shenandoah Valley Of Virginia, Noah Greenman Jan 2019

Assessing The Prevalence Of Multidrug Resistant Salmonella Enterica From Stream Sediment And Poultry Litter In The Shenandoah Valley Of Virginia, Noah Greenman

Masters Theses, 2010-2019

Multidrug resistant Salmonella enterica present in stream sediment and poultry litter represent a critical health concern. A small number of S. enterica serotypes are responsible for most lab-confirmed infections in the US each year. To assess the prevalence of these significant strains, we isolated 88 S. enterica from stream sediment and poultry litter. Sequence data for all isolates were generated using an Illumina® sequencing platform, with long-reads for some isolates from the Oxford Nanopore MinION™ used in a hybrid genome assembly approach. Isolates were typed according to their serotype and multi-locus sequence type using SeqSero/SISTR and Enterobase respectively. Antibiotic resistance …


Functional Traits Explain Variation In Chaparral Shrub Sensitivity To Altered Water And Nutrient Availability, Reina L. Nielsen, Jeremy J. James, Rebecca E. Drenovsky Jan 2019

Functional Traits Explain Variation In Chaparral Shrub Sensitivity To Altered Water And Nutrient Availability, Reina L. Nielsen, Jeremy J. James, Rebecca E. Drenovsky

2019 Faculty Bibliography

Worldwide drylands are threatened by changes in resource availability associated with global environmental change. Functional traits may help predict which species will be most responsive to these alterations in nutrient and water availability. Current functional trait work focuses on tissue construction and nutrient concentrations, but plant performance in low resource environments also may be strongly influenced by traits related to nutrient budgets and allocation. Our overall objective was to compare trait responses in a suite of serpentine and nonserpentine congener pairs from the California chaparral, a biodiverse region facing nutrient deposition and future changes in precipitation. In a common garden …


Functional Traits Explain Variation In Chaparral Shrub Sensitivity To Altered Water And Nutrient Availability, Reina Lee Nielsen, Jeremy James, Rebecca E. Drenovsky Jan 2019

Functional Traits Explain Variation In Chaparral Shrub Sensitivity To Altered Water And Nutrient Availability, Reina Lee Nielsen, Jeremy James, Rebecca E. Drenovsky

2019 Faculty Bibliography

Worldwide drylands are threatened by changes in resource availability associated with global environmental change. Functional traits may help predict which species will be most responsive to these alterations in nutrient and water availability. Current functional trait work focuses on tissue construction and nutrient concentrations, but plant performance in low resource environments also may be strongly influenced by traits related to nutrient budgets and allocation. Our overall objective was to compare trait responses in a suite of serpentine and nonserpentine congener pairs from the California chaparral, a biodiverse region facing nutrient deposition and future changes in precipitation. In a common garden …


Investigation Of Ires-Mediated Translation Of Puma Mrna: Initiation Factor Requirements And Search For Itafs, Amra Ismail Jan 2019

Investigation Of Ires-Mediated Translation Of Puma Mrna: Initiation Factor Requirements And Search For Itafs, Amra Ismail

ETD Archive

Translation initiation is the rate-limiting and tightly regulated step of protein synthesis. Cap-dependent translation initiation accounts for about 95% of cellular mRNAs. Around 3-5% of cellular mRNAs have been found to contain a cis-regulatory element (IRES) which can recruit ribosomes in a cap-independent manner. IRESs support protein synthesis under cellular stress conditions when cap-dependent translation is inhibited. Differentiation in 23A2 myoblast cells can be induced by culturing cells in serum-free differentiating media (DM). During 23A2 cellular myoblast differentiation, approximately 30% of cells undergo apoptosis as a result of stress caused by serum withdrawal in order to induce differentiation. The expression …


Towards A Developmental Biology Of Holobionts, Scott F. Gilbert Jan 2019

Towards A Developmental Biology Of Holobionts, Scott F. Gilbert

Biology Faculty Works

We do not develop as monogenomic organisms, instructed solely from the DNA and cytoplasm of the zygote. Rather, we are holobionts, symbiotic consortia containing numerous microbial genomes, whose signals are critically important for our normal development. Microbes play crucial roles in forming and maturing animal guts, immune systems, nervous systems, and reproductive organs. In some species, they regulate such developmental phenomena as the proper orientation of the anterior-posterior axis and metamorphosis. One of the biggest challenges to developmental biology, then, is studying the developmental biology of holobionts, where co-development is the rule, and where the body is seen as a …


What Is The Availability Of Iodised Salt In Supermarkets On The Island Of Ireland?, Mark Shaw, Anne P. Nugent, Breige A. Mcnulty, Janette Walton, Michaela Mchugh, Ashley Kane, Aoibhin Moore Heslin, Eoin James Morrissey, Karen Mullan, Jayne V. Woodside Jan 2019

What Is The Availability Of Iodised Salt In Supermarkets On The Island Of Ireland?, Mark Shaw, Anne P. Nugent, Breige A. Mcnulty, Janette Walton, Michaela Mchugh, Ashley Kane, Aoibhin Moore Heslin, Eoin James Morrissey, Karen Mullan, Jayne V. Woodside

Dept. of Biological Sciences Preprints

Iodine deficiency remains a global nutritional public health challenge, with evidence suggesting borderline adequate or even deficient status for UK and Irish subpopulations such as schoolgirls and pregnant women. The solution, according to the WHO, is salt iodisation, but it is not known how widely available iodised salt is in supermarkets. Iodised salt availability was determined by a shelf survey of 89 supermarket stores on the island of Ireland (n = 30 in Northern Ireland and n = 59 in the Republic of Ireland) from September 2018 to January 2019, to take into account factors such as store type, …


Does In-Vitro Fertilization Increase The Risk For Birth Defects?, Tehila Tropper Jan 2019

Does In-Vitro Fertilization Increase The Risk For Birth Defects?, Tehila Tropper

The Science Journal of the Lander College of Arts and Sciences

Since 1978 when the first “petri dish” baby was born, In-Vitro Fertilization (IVF) has been used as a tool to give couples struggling with infertility the opportunity to have children. Using this method of Assisted Reproductive Technology (ART), the woman is given medication to stimulate her ovaries for the maturation of multiple eggs, which are then retrieved via needle aspiration, fertilized in a petri dish, and inserted in the uterus with the hopes of achieving a successful pregnancy. Many times IVF is completed with another technique known as Intracytoplasmic Sperm Injection (ICSI), where the sperm is injected straight into the …


The Hyperaccumulation Of Zinc In Sunflowers And Its Effect On Disease Resistance, Rayner J. Seavey Jan 2019

The Hyperaccumulation Of Zinc In Sunflowers And Its Effect On Disease Resistance, Rayner J. Seavey

Honors Undergraduate Theses

Sunflowers are considered to be a part of a group of plants known as hyperaccumulators that share the ability to accumulate high amounts of heavy metals in the above ground organs, far in excess of the levels found in other species, often without suffering any phytotoxic effects. Quantifying the effects of zinc accumulation through the lens of the elemental defense hypothesis is essential for uncovering if there is a means to increase herbivore resistance in agricultural settings without the use of external interventions such as pesticides. A greenhouse study was conducted on four widely grown commercial cultivars of sunflower. Each …


Capillary Electrophoresis Buffer Optimization For Plant Tissue Analysis, Rebekah Davis Jan 2019

Capillary Electrophoresis Buffer Optimization For Plant Tissue Analysis, Rebekah Davis

Honors Undergraduate Theses

Capillary electrophoresis (CE) is an analytical chemistry approach that allows for the efficient separation by charge of diverse classes of compounds for analysis, including secondary metabolites. The goal of this work was to optimize a buffer system for plant tissue analysis using micellar electrokinetic chromatography (MEKC), and by doing so to understand the role of buffer components in the performance of this form of capillary electrophoresis. In this experiment we implemented a factorial design to optimize buffer composition for separating plant tissue and secondary metabolites. The results of this experiment will be used to optimize a universal buffer for MEKC …


How Do Trait-Mediated Non-Lethal Effects Of Predation Affect Population-Level Performance Of Mosquitoes?, Karthikeyan Chandrasegaran, Steven A. Juliano Jan 2019

How Do Trait-Mediated Non-Lethal Effects Of Predation Affect Population-Level Performance Of Mosquitoes?, Karthikeyan Chandrasegaran, Steven A. Juliano

Faculty Publications – Biological Sciences

Non-lethal, trait-mediated effects of predation impact prey behavior and life-history traits. Studying how these effects in turn influence prey demography is crucial to understand prey life-history evolution. Mosquitoes are important vectors that claim several million lives every year worldwide by transmitting a range of pathogens. Several ecological factors affect life-history traits of both larval and adult mosquitoes, creating effects that cascade to population-level consequences. Few studies have comprehensively explored the non-lethal effects of predation and its interactions with resources and competition on larval, adult, and population traits of mosquitoes. Understanding these interactions is important because the effects of predation are …


Coaggregation Patterns And Surface Characteristics Of Lactobacillus Rhamnosus Gg Under Varying Nutrient Conditions, Amanda Rielinger Jan 2019

Coaggregation Patterns And Surface Characteristics Of Lactobacillus Rhamnosus Gg Under Varying Nutrient Conditions, Amanda Rielinger

Senior Honors Theses and Projects

Lactobacillus rhamnosus GG (LGG) is one of the best studied probiotic organisms. The ability of probiotics to adhere to other microorganisms and the intestinal epithelium is thought to play a major role in their protective functions. Coaggregation is an important mechanism for biofilm formation by microorganisms. The goals of this study were to examine the ways in which nutrient variation affects intercellular interactions between LGG and other gut microbes and the hydrophobic character of LGG. We hypothesized that nutritional variation may affect the ability of LGG to coaggregate and form biofilms and thus affect its probiotic characteristics and ability to …


Preferences Of Specialist And Generalist Mammalian Herbivores For Mixtures Versus Individual Plant Secondary Metabolites, Jordan D. Nobler, Jennifer S. Forbey Jan 2019

Preferences Of Specialist And Generalist Mammalian Herbivores For Mixtures Versus Individual Plant Secondary Metabolites, Jordan D. Nobler, Jennifer S. Forbey

Biology Faculty Publications and Presentations

Herbivores that forage on chemically defended plants consume complex mixtures of plant secondary metabolites (PSMs). However, the mechanisms by which herbivores tolerate mixtures of PSMs are relatively poorly understood. As such, it remains difficult to predict how PSMs, singly or as complex mixtures, influence diet selection by herbivores. Although relative rates of detoxification of PSMs have been used to explain tolerance of PSMs by dietary specialist herbivores, few studies have used the rate of detoxification of individual PSMs to understand dietary preferences of individual herbivores for individual versus mixtures of PSMs. We coupled in vivo experiments using captive feeding trials …


The Purple Plague: Effect Of High Intensity Grazing Post Fire On Purple Threeawn Cover And Reproductive Effort And Prairie Dog Responses, Justin Roemer Jan 2019

The Purple Plague: Effect Of High Intensity Grazing Post Fire On Purple Threeawn Cover And Reproductive Effort And Prairie Dog Responses, Justin Roemer

Master's Theses or Doctor of Nursing Practice

Purple threeawn (Aristida purpurea Nutt.) is a native warm-season bunchgrass found in western Kansas on The Nature Conservancy’s Smoky Valley Ranch and across rangelands of western North America. Upon reaching maturity, grazing/clipping pressure decreases for this bunchgrass due to poor forage quality and extreme unpalatability for cattle (Bos taurus) and Black-tailed prairie dogs (Cynomys ludovicianus). This decrease in grazing/clipping has led to development of near monocultures which cause negative impacts to the prairie ecosystem including decreases in rangeland quality and suitable habitat for prairie dogs, a keystone species. This decrease in prairie dog habitat …


Influence Of Sporulation And Germination Behavior Of Bacillus Licheniformis On Microbial Quality Of Skim Milk Powder, Nancy Awasti Jan 2019

Influence Of Sporulation And Germination Behavior Of Bacillus Licheniformis On Microbial Quality Of Skim Milk Powder, Nancy Awasti

Electronic Theses and Dissertations

This dissertation includes modeling of sporulation and germination behavior of Bacillus licheniformis strains during raw milk holding, tracking the survival of sporeformers and spores of high sporulating Bacillus strain during skim milk powder manufacturing. In addition, preliminary studies were done to standardize a rapid spore detection technique by using ratiometric fluorescence. To understand the role of strain variability during raw milk holding conditions, population dynamics of two strains of Bacillus licheniformis, ATCC 6634 and 14580, were modeled as a function of temperature (4.0 -12.0˚ C) and duration (0 - 72 h), using regression analysis. Based on initial spiking of vegetative …


Effects Of Resistant Starch Type 4 On Gut Microbiota Derived Medium And Long Chain Fatty Acids And Bile Acids, Elizabeth Nagel Jan 2019

Effects Of Resistant Starch Type 4 On Gut Microbiota Derived Medium And Long Chain Fatty Acids And Bile Acids, Elizabeth Nagel

Electronic Theses and Dissertations

The human gastrointestinal tract is populated by a diverse and mutualistic microbial flora that has profound effect on human health. Identification and characterization of microbial-metabolites produced in response to human dietary intakes may provide insights into host-microbiota interactions to better understand the role of the microbiota in human health and diseases. However, much of this interaction is poorly understood as many metabolites remain unidentified or understudied at the present time. Starch is ubiquitously present in human diet and is a major energy source to all living cells. Resistant starch (RS) is the starch portion that is not absorbed in the …


Effects Of Inbreeding On Life-History Traits And Sexual Competency In Decorated Crickets, Scott K. Sakaluk, Jeannine Oldzej, Christine J. Poppe, Jenny L. Harper, Ian G. Rines, Kylie J. Hampton, Kristin R. Duffield, John Hunt, Ben M. Sadd Jan 2019

Effects Of Inbreeding On Life-History Traits And Sexual Competency In Decorated Crickets, Scott K. Sakaluk, Jeannine Oldzej, Christine J. Poppe, Jenny L. Harper, Ian G. Rines, Kylie J. Hampton, Kristin R. Duffield, John Hunt, Ben M. Sadd

Faculty Publications – Biological Sciences

Although inbreeding depression in life-history traits has been well characterized, inbreeding effects on mating behaviour and sexually selected traits have been less well studied. Here, we assess levels of inbreeding depression in a number of fitness-related reproductive parameters of female decorated crickets, Gryllodes sigillatus. We predicted that due to direct negative effects of inbreeding and a potential trade-off between reproduction and current survival, as suggested by effects of inbreeding on immunity, inbred females would show significantly reduced reproductive output compared with outbred females. We also examined sex-specific effects of inbreeding on mating competency, focusing specifically on the female's decision …


Stability Of Temperate Coral Astrangia Poculata Microbiome Is Reflected Across Different Sequencing Methodologies, Dawn B. Goldsmith, Zoe A. Pratte, Koty H. Sharp Jan 2019

Stability Of Temperate Coral Astrangia Poculata Microbiome Is Reflected Across Different Sequencing Methodologies, Dawn B. Goldsmith, Zoe A. Pratte, Koty H. Sharp

Arts & Sciences Faculty Publications

The microbiome of the temperate coral Astrangia poculata was first described in 2017 using next-generation Illumina sequencing to examine the coral’s bacterial and archaeal associates across seasons and among hosts of differing symbiotic status. To assess the impact of methodology on the detectable diversity of the coral’s microbiome, we obtained near full-length Sanger sequences from clone libraries constructed from a subset of the same A. poculata samples. Eight samples were analyzed: two sets of paired symbiotic (brown) and aposymbiotic (white) colonies collected in the fall (September) and two sets collected in the spring (April). Analysis of the Sanger sequences revealed …


Spatial And Temporal Distributions Of Larval Clupeid Fishes In A Tidal Freshwater Marsh Complex, Philip Reid Anderson Jan 2019

Spatial And Temporal Distributions Of Larval Clupeid Fishes In A Tidal Freshwater Marsh Complex, Philip Reid Anderson

Theses and Dissertations

Tidal freshwater wetlands provide valuable ecosystem services such as nutrient cycling, coastal flood control, and reproductive habitat for early life stages of economically and ecologically important fishes. The nutrient rich environments that tidal freshwater wetlands occur in support high levels of primary productivity of phytoplankton and vegetation that provide essential reproductive habitat for anadromous clupeid fishes (Alosa spp., Brevoortia tyrannus) in Chesapeake Bay tributaries. Seasonal retention of clupeid eggs and larvae within tidal freshwater habitats may enhance early growth, survival, and year class strength. The primary goal of the present study was to characterize the relative importance of …