Digital Commons Network^™

Exploration Of Antimicrobial And Cell Proliferation Properties Of Nanofibers Incorporating Nopal (O. Cochenillifera) Extract, Cristobal Rodriguez

Theses and Dissertations

This study focused on the fabrication of Forcespinning® nanofibers composed of Opuntia cochenillifera, ‘nopal’, mucilage (N) extract, chitosan (CH), and pullulan (PL) (N/CH/PL) were developed with an optimum fiber average diameter of 406±127 nm, and studied for their ability to sustain adhesion and proliferation of mouse embryonic fibroblast (NIH 3T3) cells. After a 6-day incubation period, N/CH/PL nanofibers displayed robust cell proliferation, while also exhibiting inhibitory properties through an N extract dip-coating process against gram-negative bacteria Escherichia coli in a 24 h bacterial growth study. A demonstration of integrated natural bioactive compounds with combined biodegradable polymers, provide an enhanced …

Evaluation Of Nickel Stress On Anabaena Cylindrica, Danielle L. Maragh

Seton Hall University Dissertations and Theses (ETDs)

Heavy metal contamination due to anthropogenic activities has steadily been on the rise over the past few decades. Cyanobacteria can thrive in water bodies that have been contaminated by heavy metal runoffs and are able to tolerate heavy metal stress even in relatively high concentrations. Cyanobacterial species can secrete toxins which can prove harmful to humans and life stock. Due to their high adaptation to heavy metal stress, understanding the potential mechanism behind their response may lead to solutions in curbing excessive cyanobacterial growth especially in water bodies that serves as sources of drinking water. Anabaena spp. is a genus …

Is The Dewlap An Honest Signal Of Fighting Ability In The Male Green Anole (Anolis Carolinensis)?, Alexia Hughes

Graduate Theses

Signals are morphological or behavioral traits that an individual uses to influence the behavior or actions of another. These signals can be used in male-male competition, in which male secondary sexual traits act as a signal of his fighting ability. Animal signals are considered honest when the signal reliably indicates a specific trait or condition of the individual. The genus Anolis, comprised of over 400 species that occupy the tropics and the southeastern United States, utilize aggressive signaling prior to physical combat. Research on several tropical species of anole indicates that the size of their dewlap can act as an …

Effects Of Environmentally Relevant Concentrations Of 4-Methyl-1- Cyclohexanemethanol (Mchm) On General Esterase And Glutathione-S-Transferase Activity In Fathead Minnows (Pimephales Promela), Emily De Wind

Research in Biology

Abstract

In 2014, several thousand gallons of coal-processing chemicals, which included 4-methyl-1- cyclohexanemethanol (MCHM), spilled into the Elk River in West Virginia. The location of this spill in West Virginia’s chemical valley is historically significant in defining the exploitations of people residing in this area. The concentration of crude MCHM in the river was approximated at 0.15 µg/L. Although some initial studies did consider the effects of MCHM exposure on humans, little attention has been given to aquatic wildlife. In this project, I will expose fathead minnows, Pimephales promelas, an environmentally relevant species, to concentrations of MCHM between zero …

Genetically Modified Organisms Are Important But Also Controversial, Masen Pelletier

Thinking Matters Symposium

Genetically modified organisms (GMOs) are organisms containing a set or multiple sets of genes that have been introduced into the organism using laboratory techniques. In the instance of agriculture, the genes introduced provide advantages for the farmer and consumer by yielding plants that produce greater yields, longer growing seasons, longer shelf lives, greater concentrations of vitamins or add additional vitamins that are novel to the plant. There are two general ways that GM (genetically modified) crops are produced: the addition or removal of genes. This review focuses on an example of each strategy. An example of the latter is GM …

Perivascular Adipose Tissue In Relation To Diet, Thermogenesis And Cardiovascular Health, Ginger Paquette, Caitlin Stieber, Ashely Soucy, Benjamin Tero, Lucy Liaw

Thinking Matters Symposium

Adipose tissue is a diverse and crucial component to vascular health due to its role in energy storage and heat production. The primary function of white adipose tissue (WAT) is energy storage while the function of mitochondria-rich brown adipose tissue (BAT) is heat production. Perivascular adipose tissue (PVAT), which surrounds blood vessels, contains both WAT and BAT adipocytes. Dietary calorie restriction is associated with increased lifespan with decreased adiposity. Increased prevalence of WAT-like PVAT, due to high-fat diets and obesity, leads to increased metabolic disfunction and cardiovascular-disease. We used a calorie-restriction model in C57BL6/J mice to test the hypothesis that …

Black-Capped Chickadee (Parus Atricapillus) Food Preference, Isabelle Gilbert

Thinking Matters Symposium

All animals must make decisions on where to find, select, capture, and eat food. These factors contribute to a species’ food preference, such as making decisions on what food to eat based on calories. Optimal foraging theory predicts how an animal behaves when searching for food, including optimization of food type, patch choice, time spent in patches, and movement. All animals also make decisions based on predation risk by avoiding predators and making trade-offs on time spent searching for, pursuing, and handling food. The objective of my study is to examine food preference of black-capped chickadees (Parus atricapillus). Birds may …

Investigation Of The Effects Of Curcumin On Woodsmoke-Induced Inflammation, Melissa Lewis

Honors Theses

Inflammatory diseases caused by biomass smoke exposure and indoor air pollution affect millions of people worldwide. These diseases can be caused by the burning of biomass (plastic, wood, rubber, tobacco, etc.) for light or heat. Acrolein is a common indoor and outdoor pollutant from tobacco smoke or organic combustion. Wood smoke is hypothesized to cause inflammation since it contains high concentrations of particulate matter and gaseous compounds and are similar in size to other well-known damaging particles. Natural remedies, like curcumin, are hypothesized to be a natural remedy for combating inflammation. The goal of this research was to investigate the …

Restructuring The Gut Microbiota By Intermittent Fasting Lowers Blood Pressure, Huanan Shi, Bojun Zhang, Taylor Abo-Hamzy, James W Nelson, Chandra Shekar R Ambati, Joseph F Petrosino, Robert M Bryan, David J Durgan

Faculty, Staff and Students Publications

Rationale:

In recent years, it has been demonstrated that a pathological change in the gut microbiota, termed gut dysbiosis, can be an underlying factor for the development of hypertension. Prevention of this dysbiosis can attenuate or abolish hypertension. Translational mechanisms to prevent gut dysbiosis as well as understanding of the mechanisms linking gut dysbiosis to hypertension are lacking.

Objective:

We first examined the efficacy of intermittent fasting (IF) in altering the gut microbiota and lowering blood pressure (BP). Next, we utilized a multi-omics approach to examine microbial influenced metabolites that may serve as the link between the gut microbiota and …

Optimization Of Grow-Out Of Bouldering Coral Microfragments: Land Vs. Offshore Nursery, Ashlee A. Steinberg

All HCAS Student Capstones, Theses, and Dissertations

Corals along the Florida Reef Tract and the wider Caribbean have been declining for decades. Low densities of adult colonies hinder the ability of corals to replenish themselves through sexual reproduction, thus reef managers are focusing on restoration actions that increase coral biomass. Microfragmentation is a way to quickly increase the biomass of bouldering corals by cutting the coral into small pieces which forces the coral to allocate its resources away from reproduction and back into growth, increasing its growth rate. This study assessed the optimal location for grow-out (land vs. offshore nursery) and its synergy with size of fragment …

The Interconnectivity Of Parkinson's Disease And Type Two Diabetes Mellitus, Erica Olfson

Honors Scholars Collaborative Projects

This thesis is about the biochemical connection between Parkinson's disease and Type II Diabetes Mellitus.

Blue Jay Call Profile In Disparate Environments, Daniel A. Gesualdi

Pell Scholars and Senior Theses

Many species of birds have been observed to develop higher-pitched calls in urban environments; however local Blue Jays are unstudied (Mockford and Marshall, 2009). The urban environment's high noise pollution imposes a significant fitness challenge; then, the urban Jay calls will be different (higher) than rural Jay(Parris and Schneider, 2009). A series of recordings were taken in rural and urban environments; it was clear that there is a significant increase in maximum and minimum kHz in urban jays than rural jays. These results suggest that blue jays alter their calls to accommodate the environmental factors in urban settings similar to …

2014 Health Demographics Of A Rural Nicaraguan Community, Andrew Guymon, Brianna Mcgowen, Emma Foster

Research Symposium

In December 2014, a team of church members and associated healthcare workers from Owasso First Assembly in Owasso, Oklahoma, provided a free, two-day clinic and health assessment to the rural community of La Ceiba Dudu in Nicaragua. Services included medical exams, basic medications, dental extractions, and over the counter reading glasses. This report is a retrospective review of the patient records documented at that clinic. We analyzed the prevalence of parasites across five age cohorts and searched for correlations between parasite diagnosis and various patient metrics, including BMI, age, and gender. We searched for a relationship between occupants per household …

Connecting Environment And Phenotype: Deciphering Mechanisms Of Diapause Entry And Exit In The Annual Killifish Austrofundulus Limnaeus, Erin Monica Davis

Dissertations and Theses

Embryonic development is complex, dynamic, and dependent on environmental factors. Mechanisms of sensing and integrating environmental stimuli are diverse, and understanding these mechanisms in extant species can elucidate how complex phenotypes emerge from genomic information expressed in an environmental context. In Austrofundulus limnaeus, an annual killifish with alternative developmental trajectories, light and temperature are vital factors that determine if an embryo will enter a state of diapause. We hypothesize that embryos of A. limnaeus use the vitamin D₃ signaling pathway as a vehicle to incorporate these ecological signals into their developmental programing. Here we provide evidence of …

Analysis Of Interacting Proteins Of Zinc Finger Protein 410, Feifan Xu, Gary D. Isaacs

Liberty University Research Week

Undergraduate

Basic

Locations Of Mafb Genes In Mice Genome, Joshua Sparks, Gary D. Isaacs, Michael S. Price

Liberty University Research Week

Undergraduate

Basic

Civic Agriculture In Review: Then, Now, And Future Directions, Allison Kaika, Alexis Racelis

School of Integrative Biological & Chemical Sciences (Formerly Dept. of Biology)

"Civic agriculture," a term first coined by rural sociologist Thomas Lyson, refers to forms of agriculture that occur on a local level, from production to consumption, and are linked to a community's social and economic development. Sixteen years since its original articulation, the term "civic agriculture" has taken on greater significance in research, political activism, and community organizing. Grown from the roots of civic community theory, civic agriculture functions as a new branch of civic community theory that is ripe for theorization. In revisiting the foundations of the term, this review paper seeks to consolidate current and future research in …

Enamel Corrosion Levels On Squirrel Teeth From The Fox Canyon Locality, Pliocene Of Kansas, Emma Tennyson

Honors Theses

The enamel of teeth may be corroded after death, especially if the teeth pass through the digestive tract of a predator with low gastric pH. This study characterized enamel corrosion of fossil squirrel teeth from two species (Otospermophilus rexroadensis and Ictidomys meadensis) obtained from the Fox Canyon locality of Kansas. Using microscopic imaging, I categorized teeth based on corrosion level. Overall, 45% of specimens exhibited heavy to extreme corrosion. There were no significant differences in the distribution of corrosion categories between both upper and lower teeth of either species (O. rexroadensis: X2 (3, N = 140) = .562, p= .905; …

Creepy Crawly Compensation: Examining The Costs Of Ectoparasite-Induced Compensatory Growth In Late-Stage Nestlings, Elliot Lusk

Biology

When normal growth rates are suppressed, organisms may undergo a rapid period of increased growth in order to match the physical requirements of a developmental benchmark. This compensatory growth, however, is not without its costs, which can have varying consequences. In nestlings, a major benchmark is that of fledging, which requires advanced physical maturation. In previous studies, compensatory growth and some of its associated costs have been shown in prefledging nestlings but fledging-aged nestlings and the costs they may have sustained needs further study. Here we examine the costs of compensatory growth in late-stage European starling (Sturnus vulgaris) nestlings under …

Demography Of A Widespread Raptor Across Disparate Regions, Christopher J.W. Mcclure, Jessi L. Brown, Sarah E. Schulwitz, John Smallwood, Kathleen E. Farley, Jean Francois Therrien, Karl E. Miller, Karen Steenhof, Julie A. Heath

Department of Biology Faculty Scholarship and Creative Works

Demographic differences between stable and declining populations can lend insight into drivers of population decline. The American Kestrel Falco sparverius is a widespread and often-studied falcon, yet its demography is poorly understood, and the causes of observed population declines across much of North America remain unknown. Using integrated population models and sensitivity analysis, we examine how vital rates drive growth in population levels of American Kestrels at four discrete study sites – Florida, Idaho and Pennsylvania with stable nestbox occupancy, and New Jersey, where occupancy is declining. Population growth was most sensitive to changes in adult survival, yet was most …

Genes Related To Redox And Cell Curvature Facilitate Interactions Between Caulobacter Strains And Arabidopsis, Louis Berrios, Bert Ely

Faculty Publications

Bacteria play an integral role in shaping plant growth and development. However, the genetic factors that facilitate plant-bacteria interactions remain largely unknown. Here, we demonstrated the importance of two bacterial genetic factors that facilitate the interactions between plant-growth-promoting (PGP) bacteria in the genus Caulobacter and the host plant Arabidopsis. Using homologous recombination, we disrupted the cytochrome ubiquinol oxidase (cyo) operon in both C. vibrioides CB13 and C. segnis TK0059 by knocking out the expression of cyoB (critical subunit of the cyo operon) and showed that the mutant strains were unable to enhance the growth of Arabidopsis. In addition, disruption of …

The Journey To Reach Herd Immunity: The United State's Efforts Towards Controlling Sars-Cov-2, Natalina Mastroianni

Senior Honors Projects

COVID-19 is a respiratory disease caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) which emerged in December of 2019 and has been the leading global health concern since. SARS-CoV-2 is a positive sense single-stranded RNA virus coated with viral spike proteins that has led to over 500,000 deaths in the United States and is approaching 3,000,000 deaths worldwide. As COVID-19 persists globally in such high numbers, the virus has been able to undergo mutations, leading to three prominent variants: the United Kingdom variant (B.1.1.7), the South Africa variant (B.1.351), and the Brazil variant (P.1). These variants are more transmissible, …

Low Activities Of Digestive Enzymes In The Guts Of Herbivorous Grouse (Aves: Tetraoninae), Jennifer Sorensen Forbey

Biology Faculty Publications and Presentations

Avian herbivores face the exceptional challenge of digesting recalcitrant plant material while under the selective pressure to reduce gut mass as an adaptation for fight. One mechanism by which avian herbivores may overcome this challenge is to maintain high activities of intestinal enzymes that facilitate the digestion and absorption of nutrients. However, previous studies in herbivorous animals provide equivocal evidence as to how activities of digestive enzymes may be adapted to herbivorous diets. For example, “rate-maximizing” herbivores generally exhibit rapid digesta transit times and high activities of digestive enzymes. Conversely, “yield-maximizing” herbivores utilize long gut retention times and express lower …

Male-Biased Dispersal In A Fungus-Gardening Ant Symbiosis, Alix E. Matthews, Katrin Kellner, Jon N. Seal

Biology Faculty Publications and Presentations

For nearly all organisms, dispersal is a fundamental life-history trait that can shape their ecology and evolution. Variation in dispersal capabilities within a species exists and can influence population genetic structure and ecological interactions. In fungus-gardening (attine) ants, co-dispersal of ants and mutualistic fungi is crucial to the success of this obligate symbiosis. Female-biased dispersal (and gene flow) may be favored in attines because virgin queens carry the responsibility of dispersing the fungi, but a paucity of research has made this conclusion difficult. Here, we investigate dispersal of the fungus-gardening ant Trachymyrmex septentrionalis using a combination of maternally (mitochondrial DNA) …

The Effect Of A Folate Free Diet On Tumor Burden And Chemotherapy Effectiveness, Kendall E. Stalls

Senior Theses

Our research is to understand the effect of the microbiota on colorectal cancer. Evidence has been shown that the microbiota is linked to the development and progression of colorectal cancer. The research focuses on how the gut microbiome influences the effectiveness of colorectal cancer treatments. Specifically, how the composition of the microbiota and the metabolites that it secretes changes the response to chemotherapy. We also research how tumor burden can be altered through changing the composition of the gut bacteria. Our experiments are performed on APC/Min+ mice, that have been genetically altered to have colon cancer. We conducted an experiment …

Laboratory Techniques In Public Health: Bi-520 Introduction To Public Health Lab Manual, Anuradha Srivastava

Open Educational Resources

This laboratory manual for a foundations course Public Health is unique in the sense that very rarely, a foundations course in the field has a lab component offered with the lecture. This lab manual provides basic laboratory exercises that could be useful to introduce undergraduate students to the basics of public health laboratory practices.

Responses Of Soil Surface Greenhouse Gas Emissions To Nitrogen And Sulfur Fertilizer Rates To Brassica Carinata Grown As A Bio-Jet Fuel, Dwarika Bhattarai, Gandura O. Abagandura, Thandiwe Nleya, Sandeep Kumar

Agronomy, Horticulture and Plant Science Faculty Publications

Carinata (Brassica carinata A. Braun), a non-food oilseed crop and an alternative bio-jet fuel feedstock, has received attention for its potential as a low-input option for production in the semi-arid region of the Northern Great Plains of the United States. Research addressing the impacts of nitrogen (N) and sulfur (S) fertilizers on soils and greenhouse gas (GHG; CO₂, N₂O, and CH₄) emissions from carinata production are limited. Thus, objective of this study was to evaluate the impact of different rates of N and S fertilizers applied to carinata on soil properties and GHG …

Ocean Acidification Modulates The Impact Of Fluoxetine On Larval Behaviors Of Non-Target Organisms, Veronica A. Chua , '21

Senior Theses, Projects, and Awards

Emerging pollutants, such as pharmaceuticals from human waste, are continuously released into aquatic systems. Although pharmaceuticals alone can adversely impact marine organisms, the bioavailability of many pharmaceuticals are dependent on ambient physical conditions, like pH. As few studies have considered the interactive effects of pharmaceutical pollution and anthropogenic ocean acidification, this study investigated the behavioral response of larval sea urchins (Heliocidaris crassispina) and ascidians (Styela plicata) to environmentally-relevant concentrations of fluoxetine (10 and 100 ng L⁻¹) under ambient (pH 8.0) and acidified conditions (pH 7.7). Larval ascidians reared at pH 8.0 exhibited swam in slower, more …

Genes Related To Redox And Cell Curvature Facilitate Interactions Between Caulobacter Strains And Arabidopsis, Louis Berrios, Bert Ely

Faculty Publications

Bacteria play an integral role in shaping plant growth and development. However, the genetic factors that facilitate plant-bacteria interactions remain largely unknown. Here, we demonstrated the importance of two bacterial genetic factors that facilitate the interactions between plant-growth-promoting (PGP) bacteria in the genus Caulobacter and the host plant Arabidopsis. Using homologous recombination, we disrupted the cytochrome ubiquinol oxidase (cyo) operon in both C. vibrioides CB13 and C. segnis TK0059 by knocking out the expression of cyoB (critical subunit of the cyo operon) and showed that the mutant strains were unable to enhance the growth of …

Applications And Mechanisms Of Near Infrared Spectroscopy For Age Estimation In Otoliths Of Red Snapper Lutjanus Campechanus, Michelle S. Passerotti

Theses and Dissertations

Near infrared spectroscopy (NIRS) is a light spectroscopy method useful for non-invasively discriminating and quantifying chemical composition of a wide variety of substances. Recently-developed applications of NIRS to fish age estimation across a range of taxa have sparked intense interest in exploring the feasibility of its use for rapid age estimation in fisheries population management. In this pursuit, development of species-specific calibration models relating traditionally-derived age estimates (i.e., those estimated from growth band counts) to NIR spectral signatures from ageing structures is required to derive predictive models that can then estimate age from rapid scans of whole ageing structures alone. …